[Histonet] tissue processing

[Gudrun Lang]

Thank you all for your answers!
It is pretty funny for me, that we have introduced the sponges in times,
when others kicked them already off. But this was the first time, they made
really problems. And our pathologists never complained about
sponge-artefacts.
Thank you again,
Gudrun from Austria


----- Original Message -----
From: "S Ladd" <sladd <@t> hsc.usf.edu>
To: "Gudrun Lang" <gudrun.lang <@t> aon.at>
Sent: Thursday, April 01, 2004 7:24 PM
Subject: RE: [Histonet] tissue processing


> Gudrun,
> This very same thing happenned to us 2 weeks ago. We loaded approximately
> 200 cassettes on the new VIP and about 75% of them had sponges.
> Approximately 50 of the blocks were not processed at all. The 50 blocks
were
> all in the same area of the basket and they were all in the bottom basket
> not the top! The size of the tissue did not correlate with the how well
the
> specimens were processed either; some big excisions processed and some
> little shaves did not. We checked all of the solutions and we even checked
> the formalin vials from the unprocessed cases and everything was fine. It
> was a complete and total mystery. Later someone noticed that the
agitation,
> P/V AND temperature had been turned off on all of the stations on the
> program that we had run. Having a lot of sponges probably compounded the
> problem. So....try checking the P/V, agitation and temperature. What a
> frustrating experience! I never thought in a million years that someone
else
> would have had the very same problem!
> Sharron
> University of South Florida
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Gudrun
> Lang
> Sent: Thursday, April 01, 2004 5:10 AM
> To: Histonetliste
> Subject: [Histonet] tissue processing
>
>
> Hi
> We have a current problem with our routine tissue processing in the VIP.
We
> loaded 200 capsules in the container. This time we had the half of the
> capsules filled with biopsy-sponges. Usually they are only about 30% of
all.
> We do over night processing.
> The tissue surface was something like creamy and in the trimmed block the
> tissue looked wet. I was not able to get a good section (only holes in
> paraffin). But not all blocks were like this, most of the small biopsies
> worked well.
> My suggestion is, that the great amount of sponges is responsible for the
> underprocessing. I think the tissue was'nt dehydratet enough and water was
> taken over from one step to the next.
>
> Did anybody have the same experience? Please give me some input on this
> problem.
>
> thanks in advance
> Gudrun Lang
> Akh Linz, Austria
>
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