[Histonet] tissue processing

[LaFriniere, Mike]

We use TBS's filter perforated paper for all small biopsies

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun
Lang
Sent: Thursday, April 01, 2004 5:10 AM
To: Histonetliste
Subject: [Histonet] tissue processing

Hi 
We have a current problem with our routine tissue processing in the VIP.
We loaded 200 capsules in the container. This time we had the half of
the capsules filled with biopsy-sponges. Usually they are only about 30%
of all. We do over night processing.
The tissue surface was something like creamy and in the trimmed block
the tissue looked wet. I was not able to get a good section (only holes
in paraffin). But not all blocks were like this, most of the small
biopsies worked well.
My suggestion is, that the great amount of sponges is responsible for
the underprocessing. I think the tissue was'nt dehydratet enough and
water was taken over from one step to the next.

Did anybody have the same experience? Please give me some input on this
problem.

thanks in advance
Gudrun Lang
Akh Linz, Austria 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet