[Histonet]
Re: Histonet Digest, Vol 5, Issue 1 (Out of Office reply)
Rolf Brekken
Rolf.Brekken <@t> UTSouthwestern.edu
Thu Apr 1 08:09:04 CST 2004
I am out of the lab until Monday April 5. I'll return your message as
soon as I can after then. If you need to contact someone in the lab
immediately please call our lab manager, Mishel Davis, at 214 648 1462.
thanks
rolf
>>> histonet 04/01/04 08:08 >>>
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Today's Topics:
1. Mouse pancreas (Grant, Debra)
2. Re: Mouse pancreas (Jackie.O'Connor <@t> abbott.com)
3. Loeffler's Alkaline Methylene Blue (Owen, Michael P)
4. Histonet: Loeffler's Alkaline Methylene Blue (Owen, Michael P)
5. Mouse pancreas processing schedule (Grant, Debra)
6. er/pr charging (Joyce Cline)
7. Procedure help with dermal papilla (Jenny Oblander)
8. RE: er/pr charging (Sebree Linda A.)
9. RE: Fwd: Re: [Histonet] Decalcifying mouse heads (Caroline Stott)
10. LTC4 SYNTHASE, EG2 (UniPath IHC)
11. Re: Mouse pancreas (Gayle Callis)
12. RE: Procedure help with dermal papilla (Barry R Rittman)
13. Mouse pancreas processing (Grant, Debra)
14. Lectin IHC (Amrit Samra)
15. Re: Decalcifying mouse heads (KATERINA STRATI)
16. Validation-Cyto Non-Gyn to Thin Prep (DMBCMP <@t> aol.com)
17. ARISTAN STAINER (Ernestine Middleton)
18. Re: er/pr charging (Kelly Simon)
19. Re: Lectin IHC (Pablo S?nchez Quinteiro)
20. non-speficic binding of secundary biotinilated antibodies
(Bruijntjes, J.P.)
21. tissue processing (Gudrun Lang)
22. chicken breast (Edwards, R.E.)
23. RE: Validation-Cyto Non-Gyn to Thin Prep (Joe Nocito)
24. accession numbers for multiple samples from same patient
(Diana McCaig)
25. Re: Microwave for antigen retrieval (Joelle Alcock)
26. RE: accession numbers for multiple samples from same p atient
(Deltour, Douglas D.(HM2))
27. RE: er/pr charging (Hallada, Teri)
28. Re: tissue processing (Jackie.O'Connor <@t> abbott.com)
29. RE: C4d antibody on Paraffin sections (yichao wu)
30. testing list (Julien Lambrey de Souza)
----------------------------------------------------------------------
Message: 1
Date: Wed, 31 Mar 2004 13:51:11 -0600
From: "Grant, Debra" <DRG <@t> Stowers-Institute.org>
Subject: [Histonet] Mouse pancreas
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<CED81D34E37D5043A1211565277A51E542F50D <@t> exchkc02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters,
I am looking for a good tissue processing schedule for mouse pancreas. I
am currently using the following schedule and having trouble with
sectioning the pancreas, the H&E's look cracked and parched as if my
water bath is too hot. The temperature on my water bath is 42-43 degrees
Celsius, I have a thermometer also that I check the temp with. I use
Tissue Prep paraffin for infiltration and embedding.
Fixed by the researcher in 4% PFA 24 hours rinsed in distilled water 5'
x 3.
70% Alcohol- 30 min.
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour 30 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour
Thanks in advance!
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
------------------------------
Message: 2
Date: Wed, 31 Mar 2004 14:13:07 -0600
From: Jackie.O'Connor <@t> abbott.com
Subject: Re: [Histonet] Mouse pancreas
To: "Grant, Debra" <DRG <@t> Stowers-Institute.org>
Cc: Histonet <histonet <@t> pathology.swmed.edu>
Message-ID:
<OFEDF44240.7FB03FCF-ON86256E68.006EC6DD <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
The processing schedule you are using would make fibroid uterus brittle.
What is prosoft? Anyway - 30 minutes in two changes each of (ethanol)
70%, 95%, Absolute, Xylene, and 2 changes of paraffin at 55 minutes each
should do you just fine - for just pancreas - since it is so delicate.
I
use the above schedule (45 minutes each station) for routine mice tissue
and xenografts with beautiful results.
Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotherapeutics
"Grant, Debra" <DRG <@t> Stowers-Institute.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/31/2004 01:51 PM
To: "Histonet" <histonet <@t> pathology.swmed.edu>
cc:
Subject: [Histonet] Mouse pancreas
Hi Histonetters,
I am looking for a good tissue processing schedule for mouse pancreas. I
am currently using the following schedule and having trouble with
sectioning the pancreas, the H&E's look cracked and parched as if my
water bath is too hot. The temperature on my water bath is 42-43 degrees
Celsius, I have a thermometer also that I check the temp with. I use
Tissue Prep paraffin for infiltration and embedding.
Fixed by the researcher in 4% PFA 24 hours rinsed in distilled water 5'
x 3.
70% Alcohol- 30 min.
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour
Prosoft- 1 hour 30 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Clearite 3- 1 hour 15 min.
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour
Paraffin- 1 hour
Thanks in advance!
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Wed, 31 Mar 2004 15:16:58 -0500
From: "Owen, Michael P" <Michael.Owen <@t> fda.gov>
Subject: [Histonet] Loeffler's Alkaline Methylene Blue
To: 'Histonet' <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC59440775B1B84F9C81553A090398CBCE1049 <@t> orspabothell2.fda.gov>
Content-Type: text/plain
Dear Histonet Users,
I am interested in receiving all of the formulations for Loeffler's
Alkaline
Methylene Blue available. Its uses include visualization of
Corynebacterium
diphtheria and Mycobacterium leprae.
I have already visited Dr. John Kiernan's Histochemistry FAQ, the FDA
CFSAN
BAM Online Reagents Manual, the Hardy Diagnostics Web site, and the
Histonet
Archives.
If you know of any formulations different from the sources listed above,
please send them to me at your earliest convenience.
Thank you in advance for your assistance.
Sincerely,
The FDA Lab Rat
Michael P. Owen, Regulatory Microbiologist
FDA Pacific Regional Lab Northwest
22201 23rd Drive SE Bothell, WA 98021
Phone: 425-483-4865 E-Mail: michael.owen <@t> fda.gov
------------------------------
Message: 4
Date: Wed, 31 Mar 2004 12:34:15 -0800
From: "Owen, Michael P" <Michael.Owen <@t> fda.gov>
Subject: [Histonet] Histonet: Loeffler's Alkaline Methylene Blue
To: 'Histonet' <histonet <@t> pathology.swmed.edu>
Message-ID:
<DC59440775B1B84F9C81553A090398CBCE104B <@t> orspabothell2.fda.gov>
Content-Type: text/plain
Dear Histonet Users,
I am interested in receiving all of the formulations for Loeffler's
Alkaline
Methylene Blue available. Its uses include visualization of
Corynebacterium
diphtheria and Mycobacterium leprae.
I have already visited Dr. John Kiernan's Histochemistry FAQ, the FDA
CFSAN
BAM Online Reagents Manual, the Hardy Diagnostics Web site, and the
Histonet
Archives.
If you know of any formulations different from the sources listed above,
please send them to me at your earliest convenience.
Thank you in advance for your assistance.
Sincerely,
Michael P. Owen
The FDA Lab Rat
Michael P. Owen, Regulatory Microbiologist
FDA Pacific Regional Lab Northwest
22201 23rd Drive SE Bothell, WA 98021
Phone: 425-483-4865 E-Mail: michael.owen <@t> fda.gov
------------------------------
Message: 5
Date: Wed, 31 Mar 2004 14:54:55 -0600
From: "Grant, Debra" <DRG <@t> Stowers-Institute.org>
Subject: [Histonet] Mouse pancreas processing schedule
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<CED81D34E37D5043A1211565277A51E5DDDA29 <@t> exchkc02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Hi all,
For those that wanted to know Prosoft is an alcohol substitute from
Anatech and is made with:
-ether and ester derivatives of propylene glycol, and propanol. Hope
this helps!
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
------------------------------
Message: 6
Date: Wed, 31 Mar 2004 15:56:33 -0500
From: "Joyce Cline" <jcline <@t> wchsys.org>
Subject: [Histonet] er/pr charging
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003d01c41762$a7d36760$1d2a14ac <@t> wchsys.org>
Content-Type: text/plain;charset="iso-8859-1"
Does everyone use the CPT code 88342 for charging the er/pr immuno's. Or
is there another code that is used for the technical component?
***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
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------------------------------
Message: 7
Date: Wed, 31 Mar 2004 15:00:46 -0600
From: Jenny Oblander <Jenny-Oblander <@t> omrf.ouhsc.edu>
Subject: [Histonet] Procedure help with dermal papilla
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B9D4D13500D6D411B4E70002B31B5E6C031FAD43 <@t> mail.omrf.ouhsc.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hi Guys,
I need your help in tracing down a procedure, antibodies etc for
a
research project one of my investigators is doing. They would like to
differentiate keratinocytes from dermal papilla fibroblasts. Does anyone
have experience with TGF-beta-RII or IL-1-RI? Thanks Jenny
J.Oblander, HT (A.S.C.P.)
Comparative Medicine
Oklahoma Medical Research Foundation MS#32
825 NE 13th St.
Okc,Ok 73104
jenny-oblander <@t> omrf.ouhsc.edu
405-271-7083
------------------------------
Message: 8
Date: Wed, 31 Mar 2004 15:14:37 -0600
From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
Subject: RE: [Histonet] er/pr charging
To: "Joyce Cline" <jcline <@t> wchsys.org>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D6B654003615874B873E15BA680E2D22F80AD8 <@t> uwhis-xchng1.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
We used to use 88342 but now use 88361.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: Joyce Cline [mailto:jcline <@t> wchsys.org]
Sent: Wednesday, March 31, 2004 2:57 PM
To: Histonet
Subject: [Histonet] er/pr charging
Does everyone use the CPT code 88342 for charging the er/pr immuno's. Or
is there another code that is used for the technical component?
***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Thu, 01 Apr 2004 09:52:23 +1200
From: Caroline Stott <caroline.stott <@t> anatomy.otago.ac.nz>
Subject: RE: Fwd: Re: [Histonet] Decalcifying mouse heads
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.2.1.1.0.20040401094556.020deec0 <@t> anatomy.otago.ac.nz>
Content-Type: text/plain; charset="us-ascii"; format=flowed
We use 10% formic acid. We use the chemical test to check if
decalcification is complete. That is 5ml of ammonium hydroxide added to
5ml of the bone solution and add 5ml saturated ammonium oxalate. If the
solution is milky white, there is still calcium present. So keep
changing
the solution every second day until it is finished.
Caroline
Caroline Stott
Histology Service Unit
Medical School
University of Otago
Dunedin
(03) 479 7152
------------------------------
Message: 10
Date: Wed, 31 Mar 2004 14:49:55 -0700
From: "UniPath IHC" <ihc <@t> unipathllc.com>
Subject: [Histonet] LTC4 SYNTHASE, EG2
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c4176a$1ab3a6d0$4500a8c0 <@t> unipath02>
Content-Type: text/plain; charset="us-ascii"
Does anyone know where to find antibodies to LTC4 synthase or EG2?
Thanks,
Brianna Jackson, BS, QIHC
UniPath, LLC
bjackson <@t> unipathllc.com
303-512-2220
------------------------------
Message: 11
Date: Wed, 31 Mar 2004 15:48:28 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Mouse pancreas
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040331154828.00be4610 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
>Is the Prosoft a gradient? Nothing wrong with your waterbath, it is
the
processing schedule. Your little mouse tissue is processed in a
schedule
for much bigger tissues, and is probably overexposed to dehydrants.
Included is a schedule next to yours with explanation of equivalent to
our
schedule, cut back times in dehydrants, clearant and paraffins and if
still
friable, dry and cracked, cut back even more. Be sure you trim block and
soak in water, you can try warm then cold, or warm then ice water if
needed. What you are doing is removing too much of the bound water
attached to tissue proteins along with free water in tissue spaces.
>>
> 70% Alcohol- 30 min. equivalant is 70% 30 min
>30 min >Prosoft- 1 hour " " 80% 30 min
>15 min >Prosoft- 1 hour " " 95% 15 min
>15 min >Prosoft- 1 hour " " 95% 15 min
>30 min >Prosoft- 1 hour " " 95% 30 min
>30 min >Prosoft- 1 hour " " 100% 30 min
>30 min >Prosoft- 1 hour 30 min. " " 100% 30 min
>45 min or 1 hour >Clearite 3- 1 hour 15 min.
>45 min or 1 hour >Clearite 3- 1 hour 15 min.
>elminate >Clearite 3- 1 hour 15 min. or adjust Clearite changes to be
20,
20, and 45 min
>30 min >Paraffin- 1 hour
>30 min >Paraffin- 1 hour
>30 min >Paraffin- 1 hour
>30 min >Paraffin- 1 hour
>for a total of 2 hours in paraffin at no more than 60C.
>>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 12
Date: Wed, 31 Mar 2004 16:50:28 -0600
From: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] Procedure help with dermal papilla
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<566FB0B522443D43AF02D2ADBE35A6F077FD5B <@t> UTHEVS3.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Jenny
Why not use pan cytokeratin?
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jenny
Oblander
Sent: Wednesday, March 31, 2004 3:01 PM
To: Histonet (E-mail)
Subject: [Histonet] Procedure help with dermal papilla
Hi Guys,
I need your help in tracing down a procedure, antibodies etc for
a research project one of my investigators is doing. They would like to
differentiate keratinocytes from dermal papilla fibroblasts. Does anyone
have experience with TGF-beta-RII or IL-1-RI? Thanks Jenny
J.Oblander, HT (A.S.C.P.)
Comparative Medicine
Oklahoma Medical Research Foundation MS#32
825 NE 13th St.
Okc,Ok 73104
jenny-oblander <@t> omrf.ouhsc.edu
405-271-7083
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 31 Mar 2004 16:56:41 -0600
From: "Grant, Debra" <DRG <@t> Stowers-Institute.org>
Subject: [Histonet] Mouse pancreas processing
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<CED81D34E37D5043A1211565277A51E5DDDA2C <@t> exchkc02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Thanks to all for the pancreas protocols, it seems I need to shorten my
times in all stations of the processor. I will try this.
Thanks again!
Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
------------------------------
Message: 14
Date: Wed, 31 Mar 2004 15:32:20 -0800
From: "Amrit Samra" <ASamra <@t> mrl.ubc.ca>
Subject: [Histonet] Lectin IHC
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <s06ae4af.056 <@t> mail.mrl.ubc.ca>
Content-Type: text/plain; charset=US-ASCII
I am doing lectin staining for frozen guinea pig tissues. I have tried
different fixitives; bouin, 10% formalin, acetone, ethanol, B5 and
different buffers; TBS, PBS and lectin buffer. I am still not able to
get a positive staining for my known positive. The last trial I did was
fixing in bouin and washing with TBS.
any help would be greatly appreciated.
Jenny
Amrit Samra
Histology Lab,the iCAPTURE Centre
St.Paul's Hospital
1081 Burrard Street,Vancouver,BC
Canada V6Z 1Y6
Phone: 604-682-2344 extension 62703
------------------------------
Message: 15
Date: Wed, 31 Mar 2004 18:09:46 -0600
From: KATERINA STRATI <kstrati <@t> wisc.edu>
Subject: Re: [Histonet] Decalcifying mouse heads
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <658073650c1f.650c1f658073 <@t> wiscmail.wisc.edu>
Content-Type: text/plain; charset=us-ascii
Thank you to everyone for useful advice
Katerina Strati
Lambert Lab
222 McArdle Lab
1400 University Ave.
Madison, WI 53706
(608) 262-6407
kstrati <@t> wisc.edu
----- Original Message -----
From: KATERINA STRATI <kstrati <@t> wisc.edu>
Date: Tuesday, March 30, 2004 5:33 pm
Subject: [Histonet] Decalcifying mouse heads
>
>
> We are interested in decalcifying whole heads from adult mice in
> order to examine histological sections throughout the oral cavity
> and esophagus. Are you aware what would be appropriate for such a
> large piece of tissue, or how long it would take to decalcify? In
> the future we would like to have the option of performing
> immunohistochemistry on our decalcified samples. Do you have any
> experience with IHC on such large decalcified samples?
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 16
Date: Wed, 31 Mar 2004 20:13:18 EST
From: DMBCMP <@t> aol.com
Subject: [Histonet] Validation-Cyto Non-Gyn to Thin Prep
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <67.2570733f.2d9cc6ae <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello All:
The director of my lab (pathologist) and my supervisor (Cytotech)
have asked me to post this question on HistoNet.
Has anyone done validation for converting non-gyn cytology
specimens to Thin Prep? Did you run parallel samples .....how
many?,etc.
Any input will be graciously welcomed. Thanks very much.
Dannie Blake, HT
Fresno Community Hospital
Fresno, Ca.
------------------------------
Message: 17
Date: Wed, 31 Mar 2004 21:19:15 -0500 (EST)
From: Ernestine Middleton <ernestinemiddleton <@t> yahoo.ca>
Subject: [Histonet] ARISTAN STAINER
To: "'histonet <@t> pathology.swmed.edu'" <histonet <@t> pathology.swmed.edu>
Message-ID: <20040401021915.65884.qmail <@t> web41607.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi;
For those who are using the Aristan stainer; will you please send me you
procedure for gram and wathin starry.
Thank you,
Ernestine Middleton, Manager, HT/HTL
Montefiore Med. Ct.
Bronx, NY
718-920-4157
718-547-1920 fax
---------------------------------
Post your free ad now! Yahoo! Canada Personals
------------------------------
Message: 18
Date: Wed, 31 Mar 2004 22:06:24 -0800 (PST)
From: Kelly Simon <kb2drkprk <@t> yahoo.com>
Subject: Re: [Histonet] er/pr charging
To: Joyce Cline <jcline <@t> wchsys.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20040401060624.14769.qmail <@t> web20909.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
According to a story by Lisa Miller in the January
2004 CAP Today, there is a new code for ER/PR and
HER2/neu:
"CPT 2004 separates traditional tumor morphometric
analysis from semiquantitative immunohistochemistry.
New code 88361 was created to report quantitative or
semiquantitative immunohistochemistry for such
analyses as hormone receptor and HER2/neu testing. The
technical services of 88342 are incorporated into the
new code."
http://www.cap.org/apps/docs/cap_today/feature_stories/0104InWithNewCPT2004.html
Kelly Simon, HTL (ASCP)
Dynacare Laboratories
Seattle, WA
--- Joyce Cline <jcline <@t> wchsys.org> wrote:
> Does everyone use the CPT code 88342 for charging
> the er/pr immuno's. Or is there another code that is
> used for the technical component?
>
> ***** CONFIDENTIALITY NOTICE *****
> This message contains confidential information and
> is intended only for
> the individual named. If you are not the named
> addressee you should not
> disseminate, distribute or copy this e-mail. Please
> notify the sender
> immediately by e-mail if you have received this
> e-mail by mistake and
> delete this e-mail from your system.
>
> _______________________________________________
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>
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------------------------------
Message: 19
Date: Thu, 01 Apr 2004 09:39:46 +0200
From: Pablo S?nchez Quinteiro <psanquin <@t> lugo.usc.es>
Subject: Re: [Histonet] Lectin IHC
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <3.0.6.32.20040401093946.007c0b60 <@t> pop.lugo.usc.es>
Content-Type: text/plain; charset="us-ascii"
Jenny, all my encouragement after so many tests. Sure the histonetters
can
help you but it would be necessary to know a more detailed protocol and
what organ you are studying. Don't you have problem cutting Bouin or
ethanol frozen tissues? On other hand to my experience the washes
buffers
are not critical.
Regards
Pablo
------------------------------
Message: 20
Date: Thu, 1 Apr 2004 10:09:35 +0200
From: "Bruijntjes, J.P." <bruyntjes <@t> voeding.tno.nl>
Subject: [Histonet] non-speficic binding of secundary biotinilated
antibodies
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3B070848E7C2204F9DEB8BCFD767728001079CC8 <@t> ntexch1.voeding.tno.nl>
Content-Type: text/plain; charset="us-ascii"
Hi all
In the past I used a rabbit anti mouse biotinilated secondary antibody
with my lymphocyte-monoclonals on fresh frozen spleen and/or thymus
slides of the rat. But I always had to add some ul's normal rat serum to
avoid non-specific binding of this reagent to lymphocytes.
Is anyone of you aware of a good secondary step in which adding of
normal rat serum is not necessary anymore?
Joost Bruijntjes
TNO Nutrition and Food Research
PO Box 360
3700 AJ
Zeist
The Netherlands
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Message: 21
Date: Thu, 1 Apr 2004 12:10:14 +0200
From: "Gudrun Lang" <gudrun.lang <@t> aon.at>
Subject: [Histonet] tissue processing
To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001a01c417d1$87b00500$eeeea8c0 <@t> SERVER>
Content-Type: text/plain; charset="iso-8859-1"
Hi
We have a current problem with our routine tissue processing in the VIP.
We loaded 200 capsules in the container. This time we had the half of
the capsules filled with biopsy-sponges. Usually they are only about 30%
of all. We do over night processing.
The tissue surface was something like creamy and in the trimmed block
the tissue looked wet. I was not able to get a good section (only holes
in paraffin). But not all blocks were like this, most of the small
biopsies worked well.
My suggestion is, that the great amount of sponges is responsible for
the underprocessing. I think the tissue was'nt dehydratet enough and
water was taken over from one step to the next.
Did anybody have the same experience? Please give me some input on this
problem.
thanks in advance
Gudrun Lang
Akh Linz, Austria
------------------------------
Message: 22
Date: Thu, 1 Apr 2004 11:58:06 +0100
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] chicken breast
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<DC88BEDFD1FC3F468D0376A7C75465F705C74ACB <@t> saffron.cfs.le.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Recently we have had several mammary tumours in our flock of
Leicester Blue hens.I wonder if anyone out there knows of any
specific immunohistochemical markers for chicken mammary
tissue/tumours??
Many thanks
C. Sanders
------------------------------
Message: 23
Date: Thu, 1 Apr 2004 05:50:05 -0600
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Validation-Cyto Non-Gyn to Thin Prep
To: <DMBCMP <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPMEOKCCAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain; charset="us-ascii"
Dannie,
We ran side by side testing for about 5-6 specimens from each type, i.e.
5
urines, 5 breast fluids, 5 peritoneal fluids, etc. The only type we
couldn't run side by side was CSF. However, our experience with CSF was
that
there was increased cellularity captured with the Thinprep. I guess it's
up
to each individual on how comfortable they feel. Hope this helps.
Joe Nocito BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
DMBCMP <@t> aol.com
Sent: Wednesday, March 31, 2004 7:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Validation-Cyto Non-Gyn to Thin Prep
Hello All:
The director of my lab (pathologist) and my supervisor (Cytotech)
have asked me to post this question on HistoNet.
Has anyone done validation for converting non-gyn cytology
specimens to Thin Prep? Did you run parallel samples .....how
many?,etc.
Any input will be graciously welcomed. Thanks very much.
Dannie Blake, HT
Fresno Community Hospital
Fresno, Ca.
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Message: 24
Date: Thu, 1 Apr 2004 07:00:17 -0500
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] accession numbers for multiple samples from same
patient
To: histonet <@t> pathology.swmed.edu
Message-ID: <3E5A3F039F0BD8118B4700C00D002024043132 <@t> CKHA9>
Content-Type: text/plain; charset="iso-8859-1"
Can I be advised how other sites label multiple specimens or where I can
find the Canadian standard.
Are they given different numbers ie
101-biopsy small bowel
102-biopsy cecum
103-antrum biopsy
OR
101A-biopsy small bowel
101B-biopsy cecum
101C-antrum biopsy
Thanks
Diana McCaig, R.T.
------------------------------
Message: 25
Date: Thu, 01 Apr 2004 13:02:44 +0100
From: "Joelle Alcock" <plxja <@t> nottingham.ac.uk>
Subject: Re: [Histonet] Microwave for antigen retrieval
To: <AFeatherstone <@t> KaleidaHealth.Org>,<histonet <@t> pathology.swmed.edu>
Message-ID: <s06c1303.089 <@t> ccw0m1.nottingham.ac.uk>
Content-Type: text/plain; charset=US-ASCII
Hi
In a couple of methods I still am still using the microwave for antigen
retrieval. For one I microwave my paraffin sections in 1L 0.01M citrate
buffer 6.4pH for 25 minutes. However I have found similar results by
using the abcam enzymatic antigen retrieval kit which also takes less
time in all - but care must be taken as sections are more likely to
crack/disintergrate.
Joelle Alcock
>>> "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org> 03/31/04
12:03pm >>>
Are people still using the microwave for anitgen retrieval? I thought
steamers and pressure cookers pretty much replaced that method.
Annette Featherstone HT/MLT
Kaleida Health
Buffalo General Hospital
100 High St
Buffalo NY 14203
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------------------------------
Message: 26
Date: Thu, 1 Apr 2004 14:10:52 +0200
From: "Deltour, Douglas D.(HM2)" <DDDeltour <@t> sig.med.navy.mil>
Subject: RE: [Histonet] accession numbers for multiple samples from
same p atient
To: 'Diana McCaig' <dmccaig <@t> ckha.on.ca>, histonet <@t> pathology.swmed.edu
Message-ID: <D5E821DFCCCCD31188EF009027B0C23A05001073 <@t> NH-SIG-EXCH1>
Content-Type: text/plain; charset="iso-8859-1"
101A-biopsy small bowel
101B-biopsy cecum
101C-antrum biopsy
-----Original Message-----
From: Diana McCaig [mailto:dmccaig <@t> ckha.on.ca]
Sent: Thursday, April 01, 2004 2:00 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] accession numbers for multiple samples from same
patient
Can I be advised how other sites label multiple specimens or where I can
find the Canadian standard.
Are they given different numbers ie
101-biopsy small bowel
102-biopsy cecum
103-antrum biopsy
OR
101A-biopsy small bowel
101B-biopsy cecum
101C-antrum biopsy
Thanks
Diana McCaig, R.T.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 27
Date: Thu, 1 Apr 2004 07:25:05 -0500
From: "Hallada, Teri" <thallada <@t> noch.org>
Subject: RE: [Histonet] er/pr charging
To: "Kelly Simon" <kb2drkprk <@t> yahoo.com>, "Joyce Cline"
<jcline <@t> wchsys.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<A1A10CBB8994DE4EAC374CD08DDE4B95574996 <@t> MAILSERVER.noch.org>
Content-Type: text/plain; charset="US-ASCII"
I believe you must "quantitative" the results in some format. Our
pathologists here don't quantitative ER/PR only Her2, so we don't use
88361 for ER/PR only Her2. Is this correct?
Teri Hallada BS MT CT (ASCP)
thallada <@t> noch.org
> -----Original Message-----
> From: Kelly Simon [SMTP:kb2drkprk <@t> yahoo.com]
> Sent: Thursday, April 01, 2004 01:06
> To: Joyce Cline
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] er/pr charging
>
> According to a story by Lisa Miller in the January
> 2004 CAP Today, there is a new code for ER/PR and
> HER2/neu:
>
> "CPT 2004 separates traditional tumor morphometric
> analysis from semiquantitative immunohistochemistry.
> New code 88361 was created to report quantitative or
> semiquantitative immunohistochemistry for such
> analyses as hormone receptor and HER2/neu testing. The
> technical services of 88342 are incorporated into the
> new code."
>
>
http://www.cap.org/apps/docs/cap_today/feature_stories/0104InWithNewCPT2004.html
>
> Kelly Simon, HTL (ASCP)
> Dynacare Laboratories
> Seattle, WA
>
> --- Joyce Cline <jcline <@t> wchsys.org> wrote:
> > Does everyone use the CPT code 88342 for charging
> > the er/pr immuno's. Or is there another code that is
> > used for the technical component?
> >
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Message: 28
Date: Thu, 1 Apr 2004 06:26:49 -0600
From: Jackie.O'Connor <@t> abbott.com
Subject: Re: [Histonet] tissue processing
To: "Gudrun Lang" <gudrun.lang <@t> aon.at>
Cc: Histonetliste <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OFDC0807EA.9A77D79D-ON86256E69.0043B63D <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
OOooh - Flashback. I remember about 12 years ago having the exact same
problem with the VIP and running over 100 biopsies with blue pads. The
blue biopsy pads retain about 1 mL of solution - 2 biopsy pads per
cassette - 2 mL retained solution - 100 cassettes - 200 ml of retained
solution that doesn't get rinsed out well by the next solution- so
Gudrun
- your hypothesis is correct (per my experience). You could try a
thinner
biopsy pad - Surgipath's biopsy pads are about 1/2 the thickness of
other
vendors. At the time we just quit using biopsy pads and went back to
lens
paper to wrap biopsies - and the problem was gone. Also gone was a
biopsy
pad artifact from putting semi-fixed tissues (e.g. currettings) between
blue pads.
Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotherapeutics
"Gudrun Lang" <gudrun.lang <@t> aon.at>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/01/2004 04:10 AM
To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
cc:
Subject: [Histonet] tissue processing
Hi
We have a current problem with our routine tissue processing in the VIP.
We loaded 200 capsules in the container. This time we had the half of
the
capsules filled with biopsy-sponges. Usually they are only about 30% of
all. We do over night processing.
The tissue surface was something like creamy and in the trimmed block
the
tissue looked wet. I was not able to get a good section (only holes in
paraffin). But not all blocks were like this, most of the small biopsies
worked well.
My suggestion is, that the great amount of sponges is responsible for
the
underprocessing. I think the tissue was'nt dehydratet enough and water
was
taken over from one step to the next.
Did anybody have the same experience? Please give me some input on this
problem.
thanks in advance
Gudrun Lang
Akh Linz, Austria
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 29
Date: Thu, 01 Apr 2004 20:36:50 +0800
From: "yichao wu" <yichaowu <@t> hotmail.com>
Subject: [Histonet] RE: C4d antibody on Paraffin sections
To: M.Donovan <@t> alfred.org.au
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <Law10-F6d3rOE9TuHZn0002360d <@t> hotmail.com>
Content-Type: text/plain; format=flowed
Dear Donovan,
Sorry for the late reply. Actually I learned the method from a
refence,but I
could not remember the exact journal and article now.
The sections I used were acetone-methanol(1:1) fixed paraffin embedded
sections with. I have not tried on formalin-fixed paraffin-embedded
sections.But I remember that in the reference mentioned above,it reads
that
this method is applicable on formalin-fixed sections.
The procedures are not special,just add the pretreatment with 88% formic
acid.That is to say,
Dewaxing;
Draw a circle around the sections with PAP pen;
Incubate with PBS;
Incubate with 88% formic acid (diluted with ddH2O i.e. pure water) for
20
minutes in room temperature;
Incubate with 10% fetal bovine serum as the blocker (or other kinds of
block
serum);
Incubate with monoclonal mouse anti-C4d antibody (A213 from Quidel USA)
at
4C overnight;
Incubate with FITC-conjugated rabbit anti-mouse IgG antibody (from DAKO)
for
40 mins at RT;
Observe under fluorescent microscope...
Besides, it is not any advertisement for those products I mentioned! :-)
Just a little experience.
Wish it is helpful. And thank you for your attention.You are much
welcomed!
Yichao WU,Ph.D Candidate
Research Insititute of Nephrology
305 East Zhongshan Road
Nanjing 210002,P.R.China
>From: "Donovan, Mark" <M.Donovan <@t> alfred.org.au>
>To: "'yichaowu <@t> hotmail.com'" <yichaowu <@t> hotmail.com>
>Subject: C4d antibody on Paraffin sections
>Date: Tue, 30 Mar 2004 15:39:03 +1000
>
>Dear Yichao,
>
>I read your response to Carmen Loiselle regarding the Quidel antibody
to
>C4d
>with great interest.
>
>Could you provide me with more details of your protocol for using the
>antibody on formalin fixed paraffin embedded sections.
>
>I have used this antibody on frozen sections but have not had any
success
>in
>getting it to work on formalin fixed material. Your advice would be
very
>much appreciated.
>
>Regards,
>
>Mark Donovan
>Immunohistochemistry Laboratory
>Anatomical Pathology
>The Alfred Hospital
>Melbourne, Australia
>
>
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Message: 30
Date: Thu, 01 Apr 2004 14:03:03 +0000
From: "Julien Lambrey de Souza" <jlambrey <@t> hotmail.com>
Subject: [Histonet] testing list
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY14-F219kstC7tXjs0000dd72 <@t> hotmail.com>
Content-Type: text/plain
This is a test. Do not consider.
Thankyou.
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