[Histonet] tissue processing
Jackie.O'Connor <@t> abbott.com
Jackie.O'Connor <@t> abbott.com
Thu Apr 1 06:26:49 CST 2004
OOooh - Flashback. I remember about 12 years ago having the exact same
problem with the VIP and running over 100 biopsies with blue pads. The
blue biopsy pads retain about 1 mL of solution - 2 biopsy pads per
cassette - 2 mL retained solution - 100 cassettes - 200 ml of retained
solution that doesn't get rinsed out well by the next solution- so Gudrun
- your hypothesis is correct (per my experience). You could try a thinner
biopsy pad - Surgipath's biopsy pads are about 1/2 the thickness of other
vendors. At the time we just quit using biopsy pads and went back to lens
paper to wrap biopsies - and the problem was gone. Also gone was a biopsy
pad artifact from putting semi-fixed tissues (e.g. currettings) between
blue pads.
Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotherapeutics
"Gudrun Lang" <gudrun.lang <@t> aon.at>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/01/2004 04:10 AM
To: "Histonetliste" <histonet <@t> lists.utsouthwestern.edu>
cc:
Subject: [Histonet] tissue processing
Hi
We have a current problem with our routine tissue processing in the VIP.
We loaded 200 capsules in the container. This time we had the half of the
capsules filled with biopsy-sponges. Usually they are only about 30% of
all. We do over night processing.
The tissue surface was something like creamy and in the trimmed block the
tissue looked wet. I was not able to get a good section (only holes in
paraffin). But not all blocks were like this, most of the small biopsies
worked well.
My suggestion is, that the great amount of sponges is responsible for the
underprocessing. I think the tissue was'nt dehydratet enough and water was
taken over from one step to the next.
Did anybody have the same experience? Please give me some input on this
problem.
thanks in advance
Gudrun Lang
Akh Linz, Austria
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