[Histonet] Microwave for antigen retrieval

[Joelle Alcock]

Hi
 
In a couple of methods I still am still using the microwave for antigen retrieval. For one I microwave my paraffin sections in 1L 0.01M citrate buffer 6.4pH for 25 minutes. However I have found similar results by using the abcam enzymatic antigen retrieval kit which also takes less time in all - but care must be taken as sections are more likely to crack/disintergrate.
 
Joelle Alcock


>>> "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org> 03/31/04 12:03pm >>>
Are people still using the microwave for anitgen retrieval? I thought
steamers and pressure cookers pretty much replaced that method.
Annette Featherstone HT/MLT
Kaleida Health
Buffalo General Hospital
100 High St
Buffalo NY 14203




CONFIDENTIALITY NOTICE: 
This email transmission and any documents, files, 
or previous e-mail messages attached to it are 
confidential and intended solely for the use of the 
individual or entity to whom they are addressed. 
If you are not the intended recipient, or a person 
responsible for delivering it to the intended recipient, 
you are hereby notified that any further review, 
disclosure, copying, dissemination, distribution, or 
use of any of the information contained in or attached 
to this e-mail transmission is strictly prohibited. 
If you have received this message in error, please 
notify the sender immediately by e-mail, discard 
any paper copies, and delete all electronic files 
of the message. If you are unable to contact the 
sender or you are not sure as to whether you 
are the intended recipient, please e-mail 
ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777. 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet