[Histonet] non-speficic binding of secundary biotinilated antibodies
Bruijntjes, J.P.
bruyntjes <@t> voeding.tno.nl
Thu Apr 1 02:09:35 CST 2004
Hi all
In the past I used a rabbit anti mouse biotinilated secondary antibody
with my lymphocyte-monoclonals on fresh frozen spleen and/or thymus
slides of the rat. But I always had to add some ul's normal rat serum to
avoid non-specific binding of this reagent to lymphocytes.
Is anyone of you aware of a good secondary step in which adding of
normal rat serum is not necessary anymore?
Joost Bruijntjes
TNO Nutrition and Food Research
PO Box 360
3700 AJ
Zeist
The Netherlands
This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html
More information about the Histonet
mailing list