[Histonet] Re: Reply- isopropanol as dehydrator in microwave method

[John Kiernan]

Someone wrote:
> It is actually better to use isopropanol 
> as the final step between the alcohol 
> and paraffin, since it has a lower 
> boiling point, it evaporates out faster 
> than ethanol, ...

Merck Index says the boiling points
are:
    Ethanol 78.5
    Isopropanol 82.5

There has to be another reason why you
can get away without using a clearing
agent! 
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/