[Histonet] LMD ... IHC sections falling off

[mrl0627 <@t> mail.ecu.edu]

 I sometimes lose sections (4 um thick, mounted on superfrost-plus) during the counterstaining-clearing process although they survive immuno and development in DAB.  Any thoughts of what I could be doing wrong?
It's a heart breaker to lose them at the very end of the process.  I am in a university lab and do everything by hand.  Thanks, Maureen at ECU
-----Original Message-----
From: Amos Brooks &lt;amosbrooks <@t> earthlink.net&gt;
To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, 20 Oct 2003 16:20:39 -0400
Subject: [Histonet] LMD ... IHC sections falling off

Victoria,
         Firstly try thinner sections 10 um is a bit thick. Next you should 
use some type of charged, silanized or APES slides. You can either silanize 
your own slides or buy them from a vendor. Adhesives aren't necessarily 
good for IHC as they can interfere with the reaction.
Amos Brooks



&gt;Message: 5
&gt;From: victoria.hahn-stromberg <@t> orebroll.se
&gt;To: histonet <@t> lists.utsouthwestern.edu
&gt;Date: Mon, 20 Oct 2003 13:20:39 +0200
&gt;Subject: [Histonet] LMD
&gt;
&gt;Hi!
&gt;I=B4m having problems with my LMD slides.  I use 10um paraffin embedded
&gt;histological sections which I would like to stain immunohistochemically =
&gt;with
&gt;CAM, but the sections keep falling off, can anyone plesse help =
&gt;me??/Victoria
&gt;
&gt;Victoria Hahn-Str=F6mberg
&gt;MSc, PhD student
&gt;Kliniken f=F6r Patologi
&gt;Universitetssjukhuset =D6rebro
&gt;701 85 =D6rebro
&gt;tel:  019-6023644
&gt;faxnr:019-6021035


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