From Dmborel <@t> aol.com Sat Nov 1 10:46:47 2003 From: Dmborel <@t> aol.com (Dmborel@aol.com) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Full time Histotechnology position now available Message-ID: <137.270b77a7.2cd53d77@aol.com> November 1, 2003 Immediate opening for an experienced full-time histotechnologist to join our rapidly growing well equipped specialized laboratory. Enjoy a pleasant working environment in our private non-hospital setting. Topeka has a low cost of living, excellent cultural advantages, a University and a Law School, excellent medical facilities, and is an hour from the Kansas City area by automobile. Day shift, Monday thru Friday. Dermatopathology Diagnostics, A Division of Pathology Services, P.A., 5650 SW 29th Street, Topeka, Kansas 66614. (785) 272-8246 or (888) 272-8246. www.dermpathdx.com Please contact any of the following: David M. Borel, M.D., Medical Director Jan R. Rice, Office Manager Amy Abbey, Client Services -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031101/4b4b4a8c/attachment.htm From pruegg <@t> msn.com Sat Nov 1 11:33:30 2003 From: pruegg <@t> msn.com (Patsy Ruegg) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] collagen I staining Message-ID: I had good collagen I staining with antibody from U of Iowa Hybridoma Bank with Pepsin digestion on formic acid decalcified rabbit bones. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 216 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 HP-303-644-4538 HF-303-644-3377 hm email pruegg@msn.com wk email pruegg@colobio.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ----Original Message Follows---- From: "Mass Histology Service" To: "Rita Angel" , Subject: RE: [Histonet] collagen I staining Date: Fri, 31 Oct 2003 14:50:30 -0500 We've had good luck with Calbiochem's anti-collagen I (Cat. # CP17L), 1:50 following pronase pre-treatment on rabbit bones. Jim ____________________ James Staruk, HT(ASCP) Mass Histology Service A division of DVMLab, inc. www.masshistology.com -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Rita Angel Sent: Friday, October 31, 2003 2:23 PM To: histonet@pathology.swmed.edu Subject: [Histonet] collagen I staining Hello Histonetters, Someone in our lab has been trying to stain for collagen I antibody in rabbit tissue. She's tried the antibody from several different companies and has used different antigen retrievals, but has been unable to get any positive staining. The only staining she's gotten is background. These are paraffin sections. The companies she bought the antibodies from has not been helpful. She's stained with Collagen type II & III and had no trouble with these. Any suggestions would be helpful. Thank you, Rita Angel, HT University of Cincinnati _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Add MSN 8 Internet Software to your current Internet access and enjoy patented spam control and more. Get two months FREE! http://join.msn.com/?page=dept/byoa From pathologygrl <@t> yahoo.com Sat Nov 1 15:32:39 2003 From: pathologygrl <@t> yahoo.com (Jen Steinberg) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] appropriate pay for grossing techs Message-ID: <20031101213239.59248.qmail@web21506.mail.yahoo.com> Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. --------------------------------- Do you Yahoo!? Exclusive Video Premiere - Britney Spears -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031101/ba7563eb/attachment.htm From gudrun.lang <@t> aon.at Sun Nov 2 06:19:09 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] appropriate pay for grossing techs References: <20031101213239.59248.qmail@web21506.mail.yahoo.com> Message-ID: <001e01c3a13b$849474d0$0d12a8c0@SERVER> Hi Jen, I dont have an answer for you, but another question. In Austria the person, who does crossing, is a pathologist (m.d.). We have several sorts of tissue f(rom toe to sculp) and I think without a medical uni-study, it is very difficult. Even our doctors sometimes make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message ----- From: Jen Steinberg To: histonet@lists.utsouthwestern.edu Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. ------------------------------------------------------------------------------ Do you Yahoo!? Exclusive Video Premiere - Britney Spears -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031102/d487f73a/attachment.htm From David.Muskett <@t> btopenworld.com Sun Nov 2 14:45:15 2003 From: David.Muskett <@t> btopenworld.com (David Muskett) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Audit Message-ID: Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 530 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031102/88ec10c2/attachment.gif From mdpeabody <@t> yahoo.com Sun Nov 2 17:35:29 2003 From: mdpeabody <@t> yahoo.com (Maureen Peabody) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Question-in situ hybridization Message-ID: <20031102233529.28975.qmail@web41002.mail.yahoo.com> Hello, We are starting to get ready to do in situ hybridization in our lab. What are some good beginning text or reference books to buy? Thank you, Maureen Peabody HT{ASCP} Pathology Department OV/UCLA Medical Center, Sylmar, Ca. __________________________________ Do you Yahoo!? Exclusive Video Premiere - Britney Spears http://launch.yahoo.com/promos/britneyspears/ From scoop <@t> mail.nih.gov Sun Nov 2 19:23:55 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] NADPH diaphorase Message-ID: I'm going to try do some NADPH diaphorase on brain sections. I assume you can't do the technique on formalin fixed, paraffin embedded tissue, but I found a protocol in Current Protocols in Neuroscience which says you can do the technique on tissue fixed in paraformaldehyde which has been put on a slide prior to staining. Does anyone know if this would work (if not should I use floating sections?) and am I correct in assuming that paraffin embedded tissue won't work? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From JWEEMS <@t> sjha.org Sun Nov 2 19:39:41 2003 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Grossing Biopsy Specimens Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44B6D5B6@exch4.sjha.org> We were sited at out CAP inspection for not having documentation of pathologist evaluation for non-pathologists performing gross. We have Histotech's submit biopsy specimens that can be submitted in their entirety, but do not have a PA that actually does gross. I had not really thought of submitting the biopsies as actual gross and had assumed that ANP.11640 pertained to someone acutally dissecting tissue. How do you all do this? Just have the pathologist sign a form? They observe the work daily, of course. There is only an aggregate measurment given to be inserted into a templete for dictation. Thanks for your help! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Josephs Health System, Inc. -------------- next part -------------- A non-text attachment was scrubbed... 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From histolog <@t> fcv.unl.edu.ar Mon Nov 3 05:46:13 2003 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Antigen retrieval Message-ID: <001501c3a200$185f3be0$5a0cd2aa@unl.edu.ar> hello everyone i have to pepare 1 mM EDTA (pH 8.0), 100 mM Tris / 1 mM EDTA (pH 9.0) & 10 mM Tris (pH 10.0) to antigen retrieval, does anyone have a protocol ? thank you very much ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From paul.keogh <@t> dental.tcd.ie Mon Nov 3 05:50:45 2003 From: paul.keogh <@t> dental.tcd.ie (Paul Keogh) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Protocols for MMA embedded bone. Message-ID: <2C3FCB82BFFCD3118E6300A0C9E0482231347B@stack.dental.tcd.ie> Protocols or references for staining MMA embedded slices of bone would be appreciated. Paul ********************************************************************** This email is confidential and intended solely for the use of the individual to whom it is addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Dublin Dental School and Hospital. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error, please contact the sender. ********************************************************************** From renatapechova <@t> hotmail.com Mon Nov 3 06:10:26 2003 From: renatapechova <@t> hotmail.com (renata pechova) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] (no subject) Message-ID: Dear histoneters. I am a PGS student. I am interested in anatomy and physiology of plants. I am dealing with a problem of staining plant tissu with dye called "Stains-all" (provided by polysciences). I would like to use LRW sections. Does anybody have a protocol how to use this dye? All I know about the staining protocol is, that the dye should be dissolved in ethanol (i do not know the concetration). Than you. Renata Pechova renatapechova@hotmail.com _________________________________________________________________ Plan your week with MSN Weather - http://www.msn.cz/weather/ From lao_ji <@t> yahoo.com Mon Nov 3 08:43:38 2003 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Question-in situ hybridization In-Reply-To: <20031102233529.28975.qmail@web41002.mail.yahoo.com> Message-ID: <20031103144338.88810.qmail@web12504.mail.yahoo.com> We used Current protocols in molecular biology. volume2. edited by Frederick M. Ausubel et al. Printed by John Wiley & Sons, Inc. This is a huge big book. if you cannot buy this just try to borrow from science library and copy useful portion or visit some developmental Lab Web site, propably they posts some protocols. good Luck! Jimmy --- Maureen Peabody wrote: > Hello, > We are starting to get ready to do in situ > hybridization in our lab. What are some good > beginning text or reference books to buy? > Thank you, > Maureen Peabody HT{ASCP} > Pathology Department > OV/UCLA Medical Center, > Sylmar, Ca. > > > __________________________________ > Do you Yahoo!? > Exclusive Video Premiere - Britney Spears > http://launch.yahoo.com/promos/britneyspears/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Exclusive Video Premiere - Britney Spears http://launch.yahoo.com/promos/britneyspears/ From gcallis <@t> montana.edu Mon Nov 3 09:08:14 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] PMMA protocols and staining Message-ID: <3.0.6.32.20031103080814.00bd6310@gemini.msu.montana.edu> BE sure to do a search on Histonet Archives for this request. There has been a great deal of discussion along with methods put out already. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From jkiernan <@t> uwo.ca Mon Nov 3 10:24:07 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] (no subject) References: Message-ID: <3FA68127.2B4BC738@uwo.ca> Stains-all is a cyanine dye, also called DBTC. (Its full chemical name is ridiculously long.) It can impart different colours to different types of macromolecular anions, and is most often used for staining phosphoproteins on electrophoresis gels. It is seldom used for staining sections because the colours fade very rapidly (a few days in my own experience). Light accelerates the fading. The most thorough description of the technique that I know is Hasty, Smith & Kang 1983. Histochemical identification of sulfation position in chondroitin sulfate in various cartilages. J Histochem Cytochem 31: 1367-1374. The staining solution is quite complex, and you will need to read the paper carefully before trying the method. You did not state a reason for staining plant tissues with DBTC; possibly a method intended for sulphated proteoglycans in cartilage will not provide the information you are after. Having tried the dye on animal tissues I can assure you that it's no good as a general purpose stain for histology. It is appropriate only for certain specialized applications in which results are recorded at once, before the colours fade. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ _______________________________________ renata pechova wrote: > > Dear histoneters. > I am a PGS student. I am interested in anatomy and physiology of plants. > I am dealing with a problem of staining plant tissu with dye called > "Stains-all" (provided by polysciences). I would like to use LRW sections. > Does anybody have a protocol how to use this dye? All I know about the > staining protocol is, that the dye should be dissolved in ethanol (i do not > know the concetration). > Than you. > Renata Pechova > renatapechova@hotmail.com From kerney <@t> fas.harvard.edu Mon Nov 3 10:34:54 2003 From: kerney <@t> fas.harvard.edu (Ryan Kerney) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] TECAN Message-ID: <1067877294.3fa683ae710ed@webmail.fas.harvard.edu> Does any one out there have any experience with the TECAN ISH protocols? I am looking for one that I can use on paraplast embedded sections of cartilage and bone. Most of these protocols require a dehydration step before the addition of probe + hyb that includes 30 minutes of air drying. I'm wondering if there is a way to do this on the robot. Also if anyone out there has any protocols that work on the TECAn with skeletal sections I would be very interested in seeing them. thanks, Ryan -- Ryan Kerney Department of Herpetology Museum of Comparative Zoology 26 Oxford St. Cambridge, MA 02138 Office: 617-496-4065 Lab: 617-496-4632 From mcauliff <@t> umdnj.edu Mon Nov 3 11:11:43 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] NADPH diaphorase In-Reply-To: References: Message-ID: <3FA68C4F.70407@umdnj.edu> The protocol I have seen and used calls for cryostat or sliding microtome/frozen sections of paraformaldehyde-fixed material. Mount sections on slides, I don't think I ever did free-floating sections. Dehydration and/or embedding kills the enzyme. Geoff Sharon Cooperman wrote: > I'm going to try do some NADPH diaphorase on brain sections. I assume > you can't do the technique on formalin fixed, paraffin embedded > tissue, but I found a protocol in Current Protocols in Neuroscience > which says you can do the technique on tissue fixed in > paraformaldehyde which has been put on a slide prior to staining. Does > anyone know if this would work (if not should I use floating > sections?) and am I correct in assuming that paraffin embedded tissue > won't work? > > Thanks, > Sharon -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From huffpw <@t> uleth.ca Mon Nov 3 11:19:52 2003 From: huffpw <@t> uleth.ca (Phillip Huff) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Antigen retrieval In-Reply-To: <001501c3a200$185f3be0$5a0cd2aa@unl.edu.ar> References: <001501c3a200$185f3be0$5a0cd2aa@unl.edu.ar> Message-ID: <1111.142.66.42.15.1067879992.squirrel@webmail.uleth.ca> Are you looking for information on a protocol/recipe about how to make the listed solutions, or are you looking for an antigen retrieval protocol using those solutions? Phil hello everyone > > i have to pepare 1 mM EDTA (pH 8.0), 100 mM Tris / 1 mM EDTA (pH 9.0) & > 10 mM Tris (pH 10.0) to antigen retrieval, does anyone have a protocol > ? > > thank you very much > > > > ------------------------------------------------------------------- > Dr. Hugo H. Ortega (DMV, PhD) > Departament of Cellular Biology > Faculty of Veterinary Sciences > Universidad Nacional del Litoral > R.P. Kreder 2805 - Esperanza (3080) > Santa Fe - ARGENTINA > Tel. (54)3496-420639 > Fax. (54)3496-426304 > http://fcv.unl.edu.ar/histolog/ > http://fcv.unl.edu.ar/bioterio/ > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Wendy.Grom <@t> lkmc.hma-corp.com Mon Nov 3 11:50:04 2003 From: Wendy.Grom <@t> lkmc.hma-corp.com (Grom, Wendy) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Position Available Message-ID: <4A267DA106F1794DBF7819D4B7B2B69D01321C59@hma-exch.hma.com> Run your own dept in Key West FL. Full time Mon -Fri. Histotechnologits position avalaible. All aspects of routine histology and immunohistochemistry. Flordia license required. ASCP cert preferred. Please contact myself at this address or Kari Owens at 1-305-294-5531 X4737 or fax 1-305-294-3809. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031103/26425396/attachment.htm From Rita.Humphrey <@t> chsys.org Mon Nov 3 12:04:49 2003 From: Rita.Humphrey <@t> chsys.org (Rita Humphrey) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] ALABAMA SOCIETY FOR HISTOTECHNOLOGY FALL MEETING NOVEMBER 14, 200 3 Message-ID: COME JOIN US IN BIRMINGHAM, ALABAMA WORKSHOP 1 8:30-4:30 ESSENTIALS OF IMMUNO STAINING WORKSHOP 2 8:30-11:30 TECHNICAL ASPECTS OF IMMUNOHISTOCHEMISTRY WORKSHOP 3 1:30-4:30 SPECIAL STAINS PRESENTATION: THE MASTERPIECE LINE(HANDS ON) WORKSHOP 4 1:30-4:30 THE PATHOLOGY OF BRONCHOALVEOLAR LAVAGE: TIPS ON CYTOSPINS & SPECIAL STAINS RECEPTION FOR NEW OFFICERS 5:00-6:30PM LOCATION: Children's Harbor at Children's Hospital, 1600 7th Ave. South, Birmingham, AL Check out our web site for additional information: http:www.timjday.com/fallsym/fallsched.htm Or call 205-939-9639. From David.Muskett <@t> btopenworld.com Mon Nov 3 12:09:54 2003 From: David.Muskett <@t> btopenworld.com (David Muskett) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Histology Exam Questions Message-ID: Dear Histonetters I am currently trying to compile a booklet of old exam questions for staff in my department to use as a revision aid or for CPD. Does anyone have any questions or old exam papers they could send me. Thanks in advance David Muskett Liverpool, UK From gudrun.lang <@t> aon.at Mon Nov 3 12:45:45 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] appropriate pay for grossing techs References: <000001c3a192$bb083400$034dbad0@hppav> Message-ID: <001a01c3a23a$b1363af0$0d12a8c0@SERVER> Sorry, I think I have used the wrong word, but I am not so good with English lab-idioms. I consider the cutting and describtion of the encoming tissue-probes, the step before processing, is called grossing?! In our histo-lab the pathologists do the grossing of all tissue, that has to be cut in any way. The histotechs put all small biopsies (renal, gastro, lung, colon,..) in capsules. (We call it "Biopsy-making" in spite of grossing by the doctor.) I wonder, if this is the same in USA or other states, or if histotechs are also allowed to do the grossing. Gudrun Lang general hospital Linz, Austria ----- Original Message ----- From: George Cole To: 'Gudrun Lang' Sent: Sunday, November 02, 2003 11:43 PM Subject: RE: [Histonet] appropriate pay for grossing techs Gudrun, I think you and Jen Steinberg had better define the terms grossing and crossing,. There might be a bit of a communication problem here. Georgecole@ev1.net -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Sunday, November 02, 2003 4:19 AM To: Histonetliste; Jen Steinberg Subject: Re: [Histonet] appropriate pay for grossing techs Hi Jen, I dont have an answer for you, but another question. In Austria the person, who does crossing, is a pathologist (m.d.). We have several sorts of tissue f(rom toe to sculp) and I think without a medical uni-study, it is very difficult. Even our doctors sometimes make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message ----- From: Jen Steinberg To: histonet@lists.utsouthwestern.edu Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. ---------------------------------------------------------------------------- Do you Yahoo!? Exclusive Video Premiere - Britney Spears -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031103/322ab787/attachment.htm From hamdi <@t> doctor.com Mon Nov 3 13:00:16 2003 From: hamdi <@t> doctor.com (hamdi abuali) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Staining Umbilical Cord Cells Message-ID: <20031103190016.5910.qmail@mail.com> Hello all anyone aware of any specific stain to tag injected HUMAN cells for cardiac proteins with no crossreactivity with rat cardiac proteins .( I am injecting human stem cells in rat heart and trying to visualize the injected cells ) . I am also looking at DAPI and Fluorescin to stain my cells .. any thoughts here ? Thank you Hamdi Abu-Ali, MD University of South Florida - Cardiology -- __________________________________________________________ Sign-up for your own personalized E-mail at Mail.com http://www.mail.com/?sr=signup CareerBuilder.com has over 400,000 jobs. Be smarter about your job search http://corp.mail.com/careers From RossS <@t> BaylorHealth.edu Mon Nov 3 13:17:22 2003 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] CAP Insitu Hybridization questions Message-ID: Hello everyone: I'm hoping for some help here since I am new to managing Insitu Hybridization. 2 of the CAP questions are confusing to me. Keep in mind with responses that we only use FDA approved clones, no ASR's are used in this lab. ANP.22956 Is there documentation of validation of commercially available probes used? Does this mean that FDA approved probes must be validated? If so, what form should this validation take? I am thinking of pulling 10 positives and 10 negatives and putting them into a report, is this enough? ANP.22968 Has the laboratory established normal and abnormal reference ranges for each probe used, and are they reviewed at least twice/year, and adjusted if necessary? I don't even know where to begin with this question. I have no experience with this. Thanks in advance for your help. These things should have been done when these tests were brought in house I guess, but I can find no evidence of it. With CAP inspection next week I'm doing the best I can. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From pruegg <@t> colobio.com Mon Nov 3 13:32:25 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Question-in situ hybridization In-Reply-To: <20031102233529.28975.qmail@web41002.mail.yahoo.com> Message-ID: Good intro to ISH handbook Royal Microscopical Society Microscopy HandBooks 27 In Situ Hybridization A.R. Leitch, T. Schwarzacher, D. Jackson and I.J. Leitch Bios Scientific Publishers Limited, St Thomas House, Becket Street, Oxford OX1 1SJ ISBN 1 872748 48 1 I had my bookstore at the University order this for me. Best regards, Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Maureen Peabody Sent: Sunday, November 02, 2003 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question-in situ hybridization Hello, We are starting to get ready to do in situ hybridization in our lab. What are some good beginning text or reference books to buy? Thank you, Maureen Peabody HT{ASCP} Pathology Department OV/UCLA Medical Center, Sylmar, Ca. __________________________________ Do you Yahoo!? Exclusive Video Premiere - Britney Spears http://launch.yahoo.com/promos/britneyspears/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Mon Nov 3 13:37:36 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Question-in situ hybridization In-Reply-To: Message-ID: -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Monday, November 03, 2003 12:32 PM To: Maureen Peabody; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question-in situ hybridization Good intro to ISH handbook Royal Microscopical Society Microscopy HandBooks 27 In Situ Hybridization A.R. Leitch, T. Schwarzacher, D. Jackson and I.J. Leitch Bios Scientific Publishers Limited, St Thomas House, Becket Street, Oxford OX1 1SJ ISBN 1 872748 48 1 I had my bookstore at the University order this for me. Best regards, Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Maureen Peabody Sent: Sunday, November 02, 2003 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question-in situ hybridization Hello, We are starting to get ready to do in situ hybridization in our lab. What are some good beginning text or reference books to buy? Thank you, Maureen Peabody HT{ASCP} Pathology Department OV/UCLA Medical Center, Sylmar, Ca. __________________________________ Do you Yahoo!? Exclusive Video Premiere - Britney Spears http://launch.yahoo.com/promos/britneyspears/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Mon Nov 3 14:28:09 2003 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] x-ray machine Message-ID: Hello histonetters, I am in need of a new x-ray machine for my bone lab. I remember many of you have a faxitron. Could you tell me which model you have that is very basic and uses film? I need it for end point decalcification as well as x-ray whole and sliced human bones and mice-whole body and parts. The one I had sat on top of a counter. I appreciate any information you have to offer. Peggy DiCarlo Orthopedics Bone Lab Buffalo General Hospital CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From gudrun.lang <@t> aon.at Mon Nov 3 13:28:38 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] CAP? Message-ID: <006e01c3a240$ae79a440$0d12a8c0@SERVER> Please explain the abbreviation CAP to me. Gudrun Lang, Austria -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031103/4676ff4c/attachment.htm From RossS <@t> BaylorHealth.edu Mon Nov 3 14:55:54 2003 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] CAP? Message-ID: Sorry, CAP is a United States inspection agency. It is the College of American Pathologists. Most if not all US labs are inspected by CAP. It is the biggest inspection we have and it is every 2 years. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Gudrun Lang Sent: Monday, November 03, 2003 1:29 PM To: Histonetliste Subject: [Histonet] CAP? Please explain the abbreviation CAP to me. Gudrun Lang, Austria This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From hmcleod <@t> chempath.uct.ac.za Mon Nov 3 23:15:44 2003 From: hmcleod <@t> chempath.uct.ac.za (Mcleod) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] dystrophin Message-ID: <3FA73600.8DDD92C8@chempath.uct.ac.za> Dear All Would anybody be able to help with the above antibody? A researcher who has never done any IHC before bought the following Novocastra Antobodies (1) NCL-DYS1 (2) NCL-DYS2 and (3) NCL-DYS3. These however are for frozen sections and she needs to work with FFPE material. A first attempt on the ffpe sections gave no staining. Has anybody by any chance ever had the need to use them? ANY help will be greatly appreciated. Thankyou Heather McLeod From Sellis4051 <@t> aol.com Tue Nov 4 00:32:18 2003 From: Sellis4051 <@t> aol.com (Sellis4051@aol.com) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Grossing wage Message-ID: <19f.1c8b5010.2cd8a1f2@aol.com> I was trained to gross prior to getting my bachelor's degree when I had an associates degree in Histotechnology and was a HT(ASCP). They paid me the same wage that I would have gotten doing routine histology. I grossed derms, gyns, noncancerous uteri, appendix, gall bladder, hernia sacs, breast reductions, digits. Unusual cases were held aside for the pathologist to gross later in the day. The person who took my job after me had an anatomy degree but no histology experience. I believe she was paid the same as me. This was in Seattle in 1995-1998. Some universities offer a bachelor's degree in something they are calling a PA - Pathologist's Assistant. There are a couple people like this now in the Seattle area and I believe that they getting paid better than histotechs. I don't find this surprising since with the loss of histology schools, people are being trained "off the street" and aren't expecting much pay wise. So grossing has become something that is seen to need a bachelors degree (and thus higher pay) whereas histotechs are losing their prestige. (Have I opened a can of worms here?) I do not know what the cut off is for the PA to turn a case over to the pathologist. I have seen them gross cancerous uteri and breast. Sandra Ellis current grad student -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/b4a67448/attachment.htm From settembr <@t> umdnj.edu Tue Nov 4 06:16:49 2003 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] dystrophin Message-ID: Hello Heather, Sorry, but I have not used them on FFPE tissue, only frozen. I supposed you tried pretreatments? Try Dako's Target Retrieval Solution or Cell Marques Trilogy before you start your staining. Good Luck. Dana Settembre University Hospital-UMDNJ Newark, NJ >>> Mcleod 11/3/2003 9:15:44 PM >>> Dear All Would anybody be able to help with the above antibody? A researcher who has never done any IHC before bought the following Novocastra Antobodies (1) NCL-DYS1 (2) NCL-DYS2 and (3) NCL-DYS3. These however are for frozen sections and she needs to work with FFPE material. A first attempt on the ffpe sections gave no staining. Has anybody by any chance ever had the need to use them? ANY help will be greatly appreciated. Thankyou Heather McLeod _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Nov 4 07:13:13 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] N N-Dimethyl formamide Message-ID: Is anyone familiar with a source for N N-Dimethyl formamide other than Sigma? It appears that the smallest volume that they now sell is 1.0 liter. Thanks! Richard Cartun From ian.montgomery <@t> bio.gla.ac.uk Tue Nov 4 07:52:21 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:08 2005 Subject: Fwd: [Histonet] N N-Dimethyl formamide Message-ID: <6.0.0.22.2.20031104135101.02d18560@udcf.gla.ac.uk> Richard, Here in miserable rain soaked Glasgow we buy it from Merck in a 500ml bottle. Ian. >X-Mailer: Novell GroupWise Internet Agent 6.0.2 >From: "Richard Cartun" >To: >X-Scan-Signature: 4ee3b494223d39d3dd0b2fb4d8b7a7fd >Sender: histonet-admin@lists.utsouthwestern.edu >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.0.13 >List-Help: >List-Post: >List-Subscribe: , > >List-Id: For the exchange of information pertaining to histotechnology and >related fields >List-Unsubscribe: , > > >List-Archive: >Date: Tue, 04 Nov 2003 08:13:13 -0500 >X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu >Subject: [Histonet] N N-Dimethyl formamide >X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on > swlx167.swmed.edu >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.60 >X-Spam-Level: >X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) >X-SA-Exim-Scanned: Yes >X-GLA-Spam-Filter: yes >X-GLA-Spam-Score: 0.0 (/) >X-GLA-Spam-Report: > >Is anyone familiar with a source for N N-Dimethyl formamide other than >Sigma? It appears that the smallest volume that they now sell is 1.0 >liter. Thanks! > >Richard Cartun > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/3e8648a4/attachment.htm From ian.montgomery <@t> bio.gla.ac.uk Tue Nov 4 08:27:14 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:08 2005 Subject: Fwd: RE: [Histonet] N N-Dimethyl formamide Message-ID: <6.0.0.22.2.20031104142338.02d26ec0@udcf.gla.ac.uk> Ronnie, Do you never the miss the drizzle, that fine cold mist that soaks you straight down to the semmit. Ian. >From: "Houston, Ronnie" >To: "'Ian Montgomery'" >Subject: RE: [Histonet] N N-Dimethyl formamide >Date: Tue, 4 Nov 2003 09:15:46 -0500 >X-Mailer: Internet Mail Service (5.5.2653.19) >X-OriginalArrivalTime: 04 Nov 2003 14:16:55.0326 (UTC) >FILETIME=[4CE8A3E0:01C3A2DE] >X-GLA-Warning: host-lookup-failed >X-GLA-Spam-Filter: yes >X-GLA-Spam-Score: 3.5 (+++) >X-GLA-Spam-Report: 2.5 NO_DNS Sending host not DNS-registered > 0.1 HTML_FONTCOLOR_BLUE BODY: HTML font color is blue > 0.2 HTML_MESSAGE BODY: HTML included in message > 0.7 HTML_20_30 BODY: Message is 20% to 30% HTML > >I don't think I could ever come back to suffer the awful weather again. I >miss my family and Glaswegians in general (not to mention the pubs!), but >I think I'd be stringing myself up after a couple of weeks. Even in >Richmond, where we have four distinct seasons, it doesn't seem as dreich. >I've been gone too long now, although I'm proud to say my accent hasn't >changed, and I still take great delight in taking the mickey out of the >Yanks; and I still have a hot toddy every night (purely for medicinal >purposes of course) > >Aw ra best >Ronnie > > > >Ronnie Houston >Regional Histology Operations Manager >Bon Secours HealthPartners Laboratories >5801 Bremo Road >Richmond, VA 23226 >(804) 287 7972 >ronnie_houston@bshsi.com >-----Original Message----- >From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk] >Sent: Tuesday, November 04, 2003 8:52 AM >To: histonet@lists.utsouthwestern.edu >Subject: Fwd: [Histonet] N N-Dimethyl formamide > >Richard, > Here in miserable rain soaked Glasgow we buy it from Merck in a > 500ml bottle. >Ian. > > >>X-Mailer: Novell GroupWise Internet Agent 6.0.2 >>From: "Richard Cartun" >>To: >>X-Scan-Signature: 4ee3b494223d39d3dd0b2fb4d8b7a7fd >>Sender: histonet-admin@lists.utsouthwestern.edu >>X-BeenThere: histonet@lists.utsouthwestern.edu >>X-Mailman-Version: 2.0.13 >>List-Help: >>List-Post: >>List-Subscribe: , >> >>List-Id: For the exchange of information pertaining to histotechnology >>and related fields >>List-Unsubscribe: >>, >> >> >>List-Archive: >>Date: Tue, 04 Nov 2003 08:13:13 -0500 >>X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >>X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu >>Subject: [Histonet] N N-Dimethyl formamide >>X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on >> swlx167.swmed.edu >>X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.60 >>X-Spam-Level: >>X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) >>X-SA-Exim-Scanned: Yes >>X-GLA-Spam-Filter: yes >>X-GLA-Spam-Score: 0.0 (/) >>X-GLA-Spam-Report: >> >>Is anyone familiar with a source for N N-Dimethyl formamide other than >>Sigma? It appears that the smallest volume that they now sell is 1.0 >>liter. Thanks! >> >>Richard Cartun >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Dr. Ian Montgomery, >Histotechnology, >Graham Kerr Building, >Institute of Biomedical & Life Sciences, >University of Glasgow, >Glasgow, >G12 8QQ. >Tel: 0141 339 8855 >Office: 4652 >Lab: 6644. >Pager: 07625 702883 >e-mail: ian.montgomery@bio.gla.ac.uk > > >________________________________________________________________________________________________________________________________ >________________________________________________________________________________________________________________________________ > >The information in this communication is intended to be confidential to >the Individual(s) and/or Entity to whom it is addressed. >It may contain information of a Privileged and/or Confidential nature, >which is subject to Federal and/or State privacy regulations. >In the event that you are not the intended recipient or the agent of the >intended recipient, do not copy or use the information >contained within this communication, or allow it to be read, copied or >utilized in any manner, by any other person(s). Should >this communication be received in error, please notify the sender >immediately either by response e-mail or by phone at 410-442-3250, >and permanently delete the original e-mail, attachment(s), and any copies. > Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/1a6d3d12/attachment.htm From Rcartun <@t> harthosp.org Tue Nov 4 08:39:49 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Plastic slide storage tray Message-ID: I am looking for a plastic slide storage tray that holds 20 slides and has clear plastic covers (so you can see the slides in the tray). Any suggestions as to where to get them? Thanks! Richard Cartun From ian.montgomery <@t> bio.gla.ac.uk Tue Nov 4 08:54:17 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:08 2005 Subject: Fwd: [Histonet] Plastic slide storage tray Message-ID: <6.0.0.22.2.20031104145021.02d233a0@udcf.gla.ac.uk> Richard, Again look in the Merck catalogue. You'll find boxes that might suit your needs if you don't mind them being vertical on the edge. The spaces between the slots is quite wide so reading the label isn't a problem. My only complaint is that the lids are made of a brittle plastic so you have to be careful in handling. Ian. >X-Mailer: Novell GroupWise Internet Agent 6.0.2 >From: "Richard Cartun" >To: >X-Scan-Signature: 4ee3b494223d39d3dd0b2fb4d8b7a7fd >Sender: histonet-admin@lists.utsouthwestern.edu >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.0.13 >List-Help: >List-Post: >List-Subscribe: , > >List-Id: For the exchange of information pertaining to histotechnology and >related fields >List-Unsubscribe: , > > >List-Archive: >Date: Tue, 04 Nov 2003 09:39:49 -0500 >X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu >Subject: [Histonet] Plastic slide storage tray >X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on > swlx167.swmed.edu >X-Spam-Status: No, hits=0.0 required=6.5 tests=none autolearn=no version=2.60 >X-Spam-Level: >X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) >X-SA-Exim-Scanned: Yes >X-GLA-Spam-Filter: yes >X-GLA-Spam-Score: 0.0 (/) >X-GLA-Spam-Report: > >I am looking for a plastic slide storage tray that holds 20 slides and >has clear plastic covers (so you can see the slides in the tray). Any >suggestions as to where to get them? Thanks! > >Richard Cartun > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/25e187fa/attachment.htm From garygill <@t> dcla.com Tue Nov 4 08:55:37 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Plastic slide storage tray Message-ID: http://www.thermo.com/eThermo/CDA/Products/Product_Detail/1,1075,21775-117-X -1-1,00.html (Google) -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, November 04, 2003 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic slide storage tray I am looking for a plastic slide storage tray that holds 20 slides and has clear plastic covers (so you can see the slides in the tray). Any suggestions as to where to get them? Thanks! Richard Cartun _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Gladney <@t> se.amedd.army.mil Tue Nov 4 09:02:23 2003 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Key West Histo Position Message-ID: <9D41AB7C56F8304F98537ABD87B249F6626156@dasmthgbz001.amedd.army.mil> Would the person that posted the Histo Tech position on Histonet yesterday please send me an email with the contacts again? I mistakenly deleted the email. Sorry to Histonet for asking for this. My fingers were faster than my mind yesterday. Thanks, Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com From powell_sa <@t> Mercer.EDU Tue Nov 4 09:37:29 2003 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Plastic slide storage tray References: Message-ID: <00ae01c3a2e9$8e512c70$a6f2acd1@powellsa1> Surgipath has them. ----- Original Message ----- From: "Richard Cartun" To: Sent: Tuesday, November 04, 2003 9:39 AM Subject: [Histonet] Plastic slide storage tray > I am looking for a plastic slide storage tray that holds 20 slides and > has clear plastic covers (so you can see the slides in the tray). Any > suggestions as to where to get them? Thanks! > > Richard Cartun > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dbpiontek <@t> hsc.wvu.edu Tue Nov 4 10:30:19 2003 From: dbpiontek <@t> hsc.wvu.edu (Denise Bland-Piontek) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Tissue Blocks for Research and Development Message-ID: I have seen a number of emails regarding control tissue sources etc. I would like to make the suggestion of contacting your local Tissue Bank. IRB's may be obtained for research and development. Armed with an IRB approval protocol and the signing of a "No Resale Agreement" will enable most Tissue Bank's to be a good resource. Patient information is de-identified, diagnosis and quality assurance testing will insure worthy tissue blocks. Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator West Virginia University Dept. of Pathology From gentras <@t> vetmed.auburn.edu Tue Nov 4 10:36:49 2003 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:08 2005 Subject: Fwd: [Histonet] Position Available Message-ID: <5.2.0.9.0.20031104103612.00a01240@mailhost.vetmed.auburn.edu> forwarded as per request. >From: "Grom, Wendy" >To: histonet@lists.utsouthwestern.edu >X-Mailer: Internet Mail Service (5.5.2653.19) >X-Scan-Signature: 48b1c0cbd8f16abb13a7a57a07b23b80 >Sender: histonet-admin@lists.utsouthwestern.edu >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.0.13 >List-Help: >List-Post: >List-Subscribe: , > >List-Id: For the exchange of information pertaining to histotechnology and >related fields >List-Unsubscribe: , > > >List-Archive: >Date: Mon, 3 Nov 2003 12:50:04 -0500 >X-Scan-Signature: 30210518ac25ee15bb6d4f97c2fc75d4 >X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu >Subject: [Histonet] Position Available >X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on > swlx167.swmed.edu >X-Spam-Status: No, hits=0.5 required=6.5 tests=HTML_20_30,HTML_MESSAGE > autolearn=no version=2.60 >X-Spam-Level: >X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) >X-SA-Exim-Scanned: Yes > >Run your own dept in Key West FL. Full time Mon -Fri. Histotechnologits >position avalaible. All aspects of routine histology and >immunohistochemistry. Flordia license required. ASCP cert preferred. >Please contact myself at this address or Kari Owens at > >1-305-294-5531 X4737 or fax 1-305-294-3809. Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/0a753193/attachment.htm From Diane.Gladney <@t> se.amedd.army.mil Tue Nov 4 11:23:38 2003 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Thanks for Info On Key West Histo Tech Position Message-ID: <9D41AB7C56F8304F98537ABD87B249F6626158@dasmthgbz001.amedd.army.mil> Thank you to all who were so kind to send me the info on the Key West Histo Tech Position. I knew that I could count on the members of Histonet to help me out. Thanks, Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com From REEVEML <@t> shands.ufl.edu Tue Nov 4 11:28:52 2003 From: REEVEML <@t> shands.ufl.edu (Mary Reeves) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Tissue Blocks for Research and Development Message-ID: In my lab the medical director insists that every slide is labeled with a computer generated label from LIS. Some of our research clients would prefer us to only write on the slides with pencil and not place a printed label on the slide. I am curious to find out what other institutions that perform research work on patient material are doing? Mary Reeves Technical Specialist Histology 1-352-265-0680 ext 7-2113 >>> "Denise Bland-Piontek" 11/04/03 11:30AM >>> I have seen a number of emails regarding control tissue sources etc. I would like to make the suggestion of contacting your local Tissue Bank. IRB's may be obtained for research and development. Armed with an IRB approval protocol and the signing of a "No Resale Agreement" will enable most Tissue Bank's to be a good resource. Patient information is de-identified, diagnosis and quality assurance testing will insure worthy tissue blocks. Denise Bland-Piontek, HTL(ASCP) Tissue Bank & Research Administrator West Virginia University Dept. of Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From usdahisto <@t> hotmail.com Tue Nov 4 11:56:45 2003 From: usdahisto <@t> hotmail.com (T. Truscott) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Not all tissue staining... Message-ID: Karen, Ventana can be great help, but you can also check that all your reagent containers are clean and that LCS didn't get poured into the wrong one. Good Luck Tom Truscott USDA-ARS Pullman, WA >From: "Laurie Colbert" >To: "Bauer, Karen" ,"Histonet (E-mail)" > >Subject: RE: [Histonet] Not all tissue staining... >Date: Wed, 29 Oct 2003 10:47:26 -0800 > >Karen, > >We have four BenchMarks and and a NexES special stainer and whenever we >have a problem we call Ventana's customer service. If they can't >troubleshoot over the phone, they are very willing to send a service tech >out. > >-----Original Message----- >From: Bauer, Karen [mailto:Bauer.Karen@mayo.edu] >Sent: Wednesday, October 29, 2003 10:25 AM >To: Histonet (E-mail) >Subject: [Histonet] Not all tissue staining... > > >Hello to all, > >There are times when a stained IP slide will have only half of the tissue >staining positively and not other parts of the tissue, but has the same >cellularity of the part that is staining. (Did that make sense?) Same >tissue cells, but some stain and some do not. When the Pathologist asks >why >this happens, I can only come up with possible vortex mixing problems or >that the paraffin did not come out completely. We've recently switched to >the BenchMark and this has only happened a few times. I've checked the >vortex mixers, and all of the heating pads are working properly. > >Does anyone have any suggestions? > >Thanks in advance. > >Karen L. Bauer HT(ASCP) >Histology Department >Luther Hospital >715-838-3205 > > > > ********************Confidentiality Notice******************** > >This message is intended for the sole use of the individual and entity to >whom it is addressed, and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. Any >unauthorized review, use, disclosure or distribution of this email message, >including any attachment, is prohibited. If you are not the intended >recipient, please advise the sender by reply email and destroy all copies >of >the original message. Thank you. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Frustrated with dial-up? Get high-speed for as low as $26.95. https://broadband.msn.com (Prices may vary by service area.) From amarusk1 <@t> FAIRVIEW.ORG Tue Nov 4 12:26:59 2003 From: amarusk1 <@t> FAIRVIEW.ORG (ANN MARUSKA) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Not all tissue staining... Message-ID: Skipped content of type multipart/alternative-------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:ANN MARUSKA EMAIL;WORK;PREF;NGW:AMARUSK1.EAST.NORTH ORG:;LAB N:MARUSKA;ANN TEL;WORK:612-273-9119 END:VCARD From MTitford <@t> aol.com Tue Nov 4 12:41:25 2003 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Miserable rain soaked Glasgow Message-ID: <5E6292C6.1F9C3F6F.00762DB1@aol.com> Dear Dr Montgomery, I don't know why anyone lives in a place like "Miserable rain soaked" Glasgow! Here in Alabama, we are having a beautiful autumn, and we expect a mild winter - as usual! Its been several years since it was cold enough to require gloves! Mike Titford USA Pathology Mobile AL USA From LCHoward <@t> covhealth.org Tue Nov 4 12:41:35 2003 From: LCHoward <@t> covhealth.org (Howard, Lynn C.) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] embedding weights Message-ID: Does anyone know where we can get embedding weights? We had a couple, and need more for another site. Privileged/Confidential information may be contained in this message. The information contained in this message is intended only for the use of the recipient(s) named above and their co-workers who are working on the same matter. The recipient of this information is prohibited from disclosing the information to any other party unless this disclosure has been authorized in advance. If you are not intended recipient of this message or any agent responsible for delivery of the message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of this message is strictly prohibited. You should immediately destroy this message and kindly notify the sender by reply E-Mail. Please advise immediately if you or your employer does not consent to Internet E-Mail for messages of this kind. Opinions, conclusions and other information in this message that do not relate to the official business of the firm shall be understood as neither given nor endorsed by it. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/590659b2/attachment.htm From RossS <@t> BaylorHealth.edu Tue Nov 4 12:55:40 2003 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Miserable rain soaked Glasgow Message-ID: I think folks live there for the proximity to Scotch distilleries. I admit I will miss cold winters now that I have moved to Dallas. I'm one of the weird folks who enjoy winter and rainy days. I have to retrain myself to enjoy 80 degree sunny days in November now. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of MTitford@aol.com Sent: Tuesday, November 04, 2003 12:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Miserable rain soaked Glasgow Dear Dr Montgomery, I don't know why anyone lives in a place like "Miserable rain soaked" Glasgow! Here in Alabama, we are having a beautiful autumn, and we expect a mild winter - as usual! Its been several years since it was cold enough to require gloves! Mike Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Ian.Bernard <@t> LACKLAND.AF.MIL Tue Nov 4 12:57:19 2003 From: Ian.Bernard <@t> LACKLAND.AF.MIL (Bernard Ian R SSgt 59 CRES/MSROP) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Need concentration recipe for making Evans Blue stain Message-ID: <588C513CC306D611A2910003479604F906591946@fsmpls17.whmc.af.mil> Need Evans Blue stain concentration to stain or be injected in Heart tissue to stain grossly fresh tissue. Any recommendations. SSgt B -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/4c30b3e3/attachment.htm From DonnaWillis <@t> texashealth.org Tue Nov 4 13:06:40 2003 From: DonnaWillis <@t> texashealth.org (Willis, Donna) Date: Fri Sep 16 15:22:08 2005 Subject: [Histonet] Not all tissue staining... Message-ID: <5C6CBCCEB04B894C8BD15B312487F7B2205AB0@ftwex01.txhealth.org> We had the same problem and found out the hard way that it was the slides we were using. We were using Gold Plus Slides. They worked great on the Nexes but when we converted to the Benchmark and XT with the new solutions we had staining issues. We sent slides inhouse to Ventana and they researched the issue. It wasn't the instruments it was the solutions with the slides. If the slides went through Xylene and Alcohols on a automatic stainer there wasn't a problem but if we put them through the EX Prep and CC1 then the problem occured. We went to using Plus Slides and everything is wonderful. Donna Willis Histology Lab Manager Harris Methodist Fort Worth, Tx -----Original Message----- From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG] Sent: Tuesday, November 04, 2003 12:27 PM To: usdahisto@hotmail.com; laurie.colbert@huntingtonhospital.com; histonet@lists.utsouthwestern.edu; Bauer.Karen@mayo.edu Subject: RE: [Histonet] Not all tissue staining... Karen, I am with you - I would suspect the vortex mixers first. But perhaps deparaffinization is a problem. It is possible that the volume of EZ prep that goes on the slide - and deparaffinizes - might be the problem....or could it have been made up improperly......or the line have a bubble in it that needs to be primed. I agree that contacting technical service at Ventana would be the first step in having them walk you thru these procedures and troubleshoot. I've had my Benchmark for 3 years come this January - and have no complaints. Ann Ann Maruska Fairview-University Medical Center Mpls. MN 55454 amarusk1@fairview.org 612-273-9119 >>> "T. Truscott" 11/04/03 11:56AM >>> Karen, Ventana can be great help, but you can also check that all your reagent containers are clean and that LCS didn't get poured into the wrong one. Good Luck Tom Truscott USDA-ARS Pullman, WA >From: "Laurie Colbert" >To: "Bauer, Karen" ,"Histonet (E-mail)" > >Subject: RE: [Histonet] Not all tissue staining... >Date: Wed, 29 Oct 2003 10:47:26 -0800 > >Karen, > >We have four BenchMarks and and a NexES special stainer and whenever we >have a problem we call Ventana's customer service. If they can't >troubleshoot over the phone, they are very willing to send a service tech >out. > >-----Original Message----- >From: Bauer, Karen [ mailto:Bauer.Karen@mayo.edu] >Sent: Wednesday, October 29, 2003 10:25 AM >To: Histonet (E-mail) >Subject: [Histonet] Not all tissue staining... > > >Hello to all, > >There are times when a stained IP slide will have only half of the tissue >staining positively and not other parts of the tissue, but has the same >cellularity of the part that is staining. (Did that make sense?) Same >tissue cells, but some stain and some do not. When the Pathologist asks >why >this happens, I can only come up with possible vortex mixing problems or >that the paraffin did not come out completely. We've recently switched to >the BenchMark and this has only happened a few times. I've checked the >vortex mixers, and all of the heating pads are working properly. > >Does anyone have any suggestions? > >Thanks in advance. > >Karen L. Bauer HT(ASCP) >Histology Department >Luther Hospital >715-838-3205 > > > > ********************Confidentiality Notice******************** > >This message is intended for the sole use of the individual and entity to >whom it is addressed, and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. Any >unauthorized review, use, disclosure or distribution of this email message, >including any attachment, is prohibited. If you are not the intended >recipient, please advise the sender by reply email and destroy all copies >of >the original message. Thank you. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Frustrated with dial-up? Get high-speed for as low as $26.95. https://broadband.msn.com (Prices may vary by service area.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Jackie.O'Connor <@t> abbott.com Tue Nov 4 13:19:12 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Miserable rain soaked Glasgow Message-ID: I know what you mean. I was born and raised in Chicago. I remember the crisp 3 mile walk home from school during the blizzard of 1967. Due to my husband's Navy career in Honolulu, our kids had to take a school 'boat' across Pearl Harbor in the dead of winter for three years. Oh, the humanity! And it's only November. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 "Stapf, Ross" Sent by: histonet-admin@lists.utsouthwestern.edu 11/04/2003 12:55 PM To: , cc: Subject: RE: [Histonet] Miserable rain soaked Glasgow I think folks live there for the proximity to Scotch distilleries. I admit I will miss cold winters now that I have moved to Dallas. I'm one of the weird folks who enjoy winter and rainy days. I have to retrain myself to enjoy 80 degree sunny days in November now. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of MTitford@aol.com Sent: Tuesday, November 04, 2003 12:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Miserable rain soaked Glasgow Dear Dr Montgomery, I don't know why anyone lives in a place like "Miserable rain soaked" Glasgow! Here in Alabama, we are having a beautiful autumn, and we expect a mild winter - as usual! Its been several years since it was cold enough to require gloves! Mike Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/6f78c866/attachment.htm From shibaji <@t> cvrti.utah.edu Tue Nov 4 13:36:48 2003 From: shibaji <@t> cvrti.utah.edu (Shibaji Shome) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] formalin fixing Message-ID: <3B2B52FF-0EFE-11D8-BDC5-000393814D66@cvrti.utah.edu> Hi, Stupid question of the day: I would like to know how formalin fixation works. Does formalin enter the extracellular space and replace water? does it enter both extra and intracellular space? The shrinkage of tissue on formalin fixing is indicative of water loss and/or collapse of intracellular/extracellular space? Any information, pointers to references will be greatly appreciated. Details on working principles of formalin fixation will greatly, vastly appreciated. Thanks! Regards, Shibaji From juan.gutierrez <@t> christushealth.org Tue Nov 4 13:48:27 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Not all tissue staining... Message-ID: I just had that same problem resolved yesterday. I don't know if you do it too, but we cut the benchmark labels to prevent them from lifting from the unpainted part of the slide. We only cut off the little tab that has the lot no. on it. Yesterday I was in a hurry and my cuts were not as smooth as they should have been. What does that have to do with my problem you ask? If you remember from benchmark training, the solution on top of the slide creates a "dome" over the tissue section, now if the edge of the dome touches an uneven label or worst a ragged edge label, the surface tension of the dome is broken and the solution will drain down the side of your slide. I am willing to bet that you had good staining closest to the label. That is because some of the solution stays behind, but the rest of the silde dries up. I had one slide drain so fast that even the hematoxylin didn't stainned it. Like somebody mentioned before, It's always good to give Ventana a call, they are the ones who helped me through this. Good luck and sorry for rambling on. Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Willis, Donna [mailto:DonnaWillis@texashealth.org] Sent: Tue 11/4/2003 1:06 PM To: 'ANN MARUSKA'; usdahisto@hotmail.com; laurie.colbert@huntingtonhospital.com; histonet@lists.utsouthwestern.edu; Bauer.Karen@mayo.edu Cc: Subject: RE: [Histonet] Not all tissue staining... We had the same problem and found out the hard way that it was the slides we were using. We were using Gold Plus Slides. They worked great on the Nexes but when we converted to the Benchmark and XT with the new solutions we had staining issues. We sent slides inhouse to Ventana and they researched the issue. It wasn't the instruments it was the solutions with the slides. If the slides went through Xylene and Alcohols on a automatic stainer there wasn't a problem but if we put them through the EX Prep and CC1 then the problem occured. We went to using Plus Slides and everything is wonderful. Donna Willis Histology Lab Manager Harris Methodist Fort Worth, Tx -----Original Message----- From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG] Sent: Tuesday, November 04, 2003 12:27 PM To: usdahisto@hotmail.com; laurie.colbert@huntingtonhospital.com; histonet@lists.utsouthwestern.edu; Bauer.Karen@mayo.edu Subject: RE: [Histonet] Not all tissue staining... Karen, I am with you - I would suspect the vortex mixers first. But perhaps deparaffinization is a problem. It is possible that the volume of EZ prep that goes on the slide - and deparaffinizes - might be the problem....or could it have been made up improperly......or the line have a bubble in it that needs to be primed. I agree that contacting technical service at Ventana would be the first step in having them walk you thru these procedures and troubleshoot. I've had my Benchmark for 3 years come this January - and have no complaints. Ann Ann Maruska Fairview-University Medical Center Mpls. MN 55454 amarusk1@fairview.org 612-273-9119 >>> "T. Truscott" 11/04/03 11:56AM >>> Karen, Ventana can be great help, but you can also check that all your reagent containers are clean and that LCS didn't get poured into the wrong one. Good Luck Tom Truscott USDA-ARS Pullman, WA >From: "Laurie Colbert" >To: "Bauer, Karen" ,"Histonet (E-mail)" > >Subject: RE: [Histonet] Not all tissue staining... >Date: Wed, 29 Oct 2003 10:47:26 -0800 > >Karen, > >We have four BenchMarks and and a NexES special stainer and whenever we >have a problem we call Ventana's customer service. If they can't >troubleshoot over the phone, they are very willing to send a service tech >out. > >-----Original Message----- >From: Bauer, Karen [ mailto:Bauer.Karen@mayo.edu] >Sent: Wednesday, October 29, 2003 10:25 AM >To: Histonet (E-mail) >Subject: [Histonet] Not all tissue staining... > > >Hello to all, > >There are times when a stained IP slide will have only half of the tissue >staining positively and not other parts of the tissue, but has the same >cellularity of the part that is staining. (Did that make sense?) Same >tissue cells, but some stain and some do not. When the Pathologist asks >why >this happens, I can only come up with possible vortex mixing problems or >that the paraffin did not come out completely. We've recently switched to >the BenchMark and this has only happened a few times. I've checked the >vortex mixers, and all of the heating pads are working properly. > >Does anyone have any suggestions? > >Thanks in advance. > >Karen L. Bauer HT(ASCP) >Histology Department >Luther Hospital >715-838-3205 > > > > ********************Confidentiality Notice******************** > >This message is intended for the sole use of the individual and entity to >whom it is addressed, and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. Any >unauthorized review, use, disclosure or distribution of this email message, >including any attachment, is prohibited. If you are not the intended >recipient, please advise the sender by reply email and destroy all copies >of >the original message. Thank you. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Frustrated with dial-up? Get high-speed for as low as $26.95. https://broadband.msn.com (Prices may vary by service area.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including "protected health information." If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Tue Nov 4 13:49:13 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] RE: dystrophin Message-ID: <1fc8fb1f845e.1f845e1fc8fb@amc.uva.nl> Dear Heather, If you don't see anything after testing these dystrophin antibodies on FFPE tissues you may try the following (as last resort perhaps): - Good old pepsin digestion (instead of HIER) using 0.25% pepsin (Sigma) in 10 mM HCl. 15 min at 37C. - A stronger detection system. Try for example the new CSA II kit from DakoCyto. A non-biotin based peroxidase staining kit that is very powerful. Test your antibodies in steps like: 1:50 - 200 - 1000 - 5000. Good luck, Chris van der Loos Academical Medical Center Dept. of Cardiovascular Pathology Amsterdam - The Netherlands ----- Original Message ----- >From Mcleod Date Tue, 04 Nov 2003 07:15:44 +0200 To "histonet@lists.utsouthwestern.edu" Subject [Histonet] dystrophin ------------------------------------------------------------------------ Dear All Would anybody be able to help with the above antibody? A researcher who has never done any IHC before bought the following Novocastra Antobodies (1) NCL-DYS1 (2) NCL-DYS2 and (3) NCL-DYS3. These however are for frozen sections and she needs to work with FFPE material. A first attempt on the ffpe sections gave no staining. Has anybody by any chance ever had the need to use them? ANY help will be greatly appreciated. Thankyou Heather McLeod From cwscouten <@t> myneurolab.com Tue Nov 4 14:18:06 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] formalin fixing Message-ID: You should check the archives for a recent discussion on fixation. Formalin works by crosslinking proteins, wherever it finds them. The result is not subject to normal protein breakdown and decay processes. Formalin penetrates to everywhere in the tissue, albeit slowly. Anything that can be done to speed up its penetration should (ie. perfusion). Heat will not speed up penetration, but may speed up decay. Shrinkage due to formalin can be prevented. When formalin arrives at the cell wall, the first thing fixed is the ubiquitous sodium pump proteins on the outer wall. As a consequence, sodium rushes in down the gradient from the extracellular fluid, and carries in water to maintain tonicity. Cells first swell and fill the extracelllar space, to abut neighboring cells, then proteins in the membranes are crosslinked between cells. When equilibrium is reestablished, the cells shrink and pull their neighbors in with them. The shrinkage due to formalin is thus loss of extracellular space that used to be there. Replacing sodium in the extracellular fluid, while maintaining tonicity, will prevent shrinkage, and give unshrunk formalin fixed soft tissue. Perfusion with ordinary sucrose at 5%, as the prewash, pressurized if brain is to preserved, will fulfill this requirement. For an instrument to assist in doing this, see the following link: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21 Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: Shibaji Shome [mailto:shibaji@cvrti.utah.edu] Sent: Tuesday, November 04, 2003 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formalin fixing Hi, Stupid question of the day: I would like to know how formalin fixation works. Does formalin enter the extracellular space and replace water? does it enter both extra and intracellular space? The shrinkage of tissue on formalin fixing is indicative of water loss and/or collapse of intracellular/extracellular space? Any information, pointers to references will be greatly appreciated. Details on working principles of formalin fixation will greatly, vastly appreciated. Thanks! Regards, Shibaji _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Tue Nov 4 14:22:25 2003 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] disposal of tissue Message-ID: <3E5A3F039F0BD8118B4700C00D002024042F91@CKHA9> How do labs dispose of pathological waste when incineration is not available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department. Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) From eileen_dusek <@t> yahoo.com Tue Nov 4 15:38:46 2003 From: eileen_dusek <@t> yahoo.com (eileen dusek) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] couple of questions Message-ID: <20031104213846.19757.qmail@web11903.mail.yahoo.com> Hi Everyone, I have a few questions, 1. Is anyone using a "home" type vacuum for the cryostat. Of course the bags would go in the medical waste. 2. Is Alcoholic formalin OK to put on the VIP tissue processor, there is no acetic acid in the solution. I appreciate all your help. Eileen signature --------------------------------- Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031104/7d3dc941/attachment.htm From Dboyd <@t> swmedctr.com Tue Nov 4 16:01:31 2003 From: Dboyd <@t> swmedctr.com (Julian-Boyd, Dawn) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] new instrument Message-ID: Looking for an instrument to extract specific tissue from a parrafin block for tx micro array. Needs to be 13 -15 gauge approximately. Any info would be appreciated.Thanks in a dvance D.Boyd From adford <@t> compuserve.com Tue Nov 4 16:02:37 2003 From: adford <@t> compuserve.com (John Difford) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Improved Eosin Message-ID: <200311041703_MC3-1-57C5-A2DC@compuserve.com> Dear All, Does anyone remember a formula for an improved Eosin solution which was displayed on Histonet several years ago? It was said to have orginated at Johns Hopkins and contained some other red dye in addition to eosin. I want to make up some more because it was very good for frozen sections, but I have lost the instructions! Many thanks John Difford Histopathology Dept Royal Free Hospital London England, UK From michael_lafriniere <@t> memorial.org Tue Nov 4 16:24:36 2003 From: michael_lafriniere <@t> memorial.org (LaFriniere, Michael) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] new instrument Message-ID: Dawn, Check EMS (Electron Microscopy Science) they have all kinds of things for extracting tissue from blocks....not sure if this is what you are seeking....they have "unicore" tools on page 199 in their catalog. Their # is 1 800-523-5874 Regards, Michael LaFriniere PA, HT(ASCP) NSH Region III Director Pathology Manager Memorial Hospital Chattanooga TN 423-496-6117 -----Original Message----- From: Julian-Boyd, Dawn [mailto:Dboyd@swmedctr.com] Sent: Tuesday, November 04, 2003 5:02 PM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] new instrument Looking for an instrument to extract specific tissue from a parrafin block for tx micro array. Needs to be 13 -15 gauge approximately. Any info would be appreciated.Thanks in a dvance D.Boyd _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Nov 4 16:37:01 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] embedding weights Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E092@simba.kids> Lynn, Try Bayer Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Howard, Lynn C. [mailto:LCHoward@covhealth.org] Sent: Wednesday, 5 November 2003 5:42 To: 'histonet@pathology.swmed.edu' Subject: [Histonet] embedding weights Does anyone know where we can get embedding weights? We had a couple, and need more for another site. Privileged/Confidential information may be contained in this message. The information contained in this message is intended only for the use of the recipient(s) named above and their co-workers who are working on the same matter. The recipient of this information is prohibited from disclosing the information to any other party unless this disclosure has been authorized in advance. If you are not intended recipient of this message or any agent responsible for delivery of the message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of this message is strictly prohibited. You should immediately destroy this message and kindly notify the sender by reply E-Mail. Please advise immediately if you or your employer does not consent to Internet E-Mail for messages of this kind. Opinions, conclusions and other information in this message that do not relate to the official business of the firm shall be understood as neither given nor endorsed by it. ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/01138e9a/attachment.htm From cwscouten <@t> myneurolab.com Tue Nov 4 17:06:48 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] new instrument Message-ID: The product at the following link is designed for brain micropunches, but I see no reason it wouldn't work great for what you need. Do you? http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=443001&catdesc=Histology+Equipment&CatThreeID=618&CatOneID=4&subcatdesc=Other+Tissue+Dissection+Equipment&idsubcategory=189 Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: Julian-Boyd, Dawn [mailto:Dboyd@swmedctr.com] Sent: Tuesday, November 04, 2003 4:02 PM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] new instrument Looking for an instrument to extract specific tissue from a parrafin block for tx micro array. Needs to be 13 -15 gauge approximately. Any info would be appreciated.Thanks in a dvance D.Boyd _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From grantgd <@t> comcast.net Tue Nov 4 17:15:38 2003 From: grantgd <@t> comcast.net (Gordon Grant) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] RE: Looking for a lab to cut stents In-Reply-To: <20031102180001.7761.59514.Mailman@swlx167.swmed.edu> Message-ID: <000701c3a329$92a8f9c0$0400a8c0@GordonGrant> I was wondering if someone out there can give me a recommendation for a lab which can provide good quality sections of implanted stents. Specifically, we have been told the FDA likes the thick saw cut, ground and polished sections rather than microtomed sections. Thanks Gordon Grant -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, November 02, 2003 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet digest, Vol 1 #113 - 2 msgs Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-admin@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. appropriate pay for grossing techs (Jen Steinberg) 2. Re: appropriate pay for grossing techs (Gudrun Lang) --__--__-- Message: 1 Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST) From: Jen Steinberg To: histonet@lists.utsouthwestern.edu Subject: [Histonet] appropriate pay for grossing techs --0-1595893577-1067722359=:57919 Content-Type: text/plain; charset=us-ascii Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. --------------------------------- Do you Yahoo!? Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919 Content-Type: text/html; charset=us-ascii
Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers).  Would the pay be any different if the employee had an M.S.  The position is entry-level.  Thanks very much. 
 
 

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Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919-- --__--__-- Message: 2 From: "Gudrun Lang" To: "Histonetliste" , "Jen Steinberg" Date: Sun, 2 Nov 2003 13:19:09 +0100 Subject: Re: [Histonet] appropriate pay for grossing techs This is a multi-part message in MIME format. ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hi Jen, I dont have an answer for you, but another question. In Austria the = person, who does crossing, is a pathologist (m.d.).=20 We have several sorts of tissue f(rom toe to sculp) and I think without = a medical uni-study, it is very difficult. Even our doctors sometimes = make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message -----=20 From: Jen Steinberg=20 To: histonet@lists.utsouthwestern.edu=20 Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the = appropriate starting wage of a grossing tech would be for a private path = lab which works mainly on small skin, gyn, and oral specimens and = processes about 250 cases a day (between 2 grossers). Would the pay be = any different if the employee had an M.S. The position is entry-level. = Thanks very much. =20 ------------------------------------------------------------------------ -= ----- Do you Yahoo!? Exclusive Video Premiere - Britney Spears ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hi Jen,
I dont have an answer for you, but = another=20 question. In Austria the person, who does crossing, is a pathologist = (m.d.).=20
We have several sorts of tissue f(rom = toe to sculp)=20 and I think without a medical uni-study, it is very difficult. Even our = doctors=20 sometimes make mistakes and look over important details.
Is it usual in USA,  that = histotechs do=20 the crossing?
 
best wishes
Gudrun Lang
general hospital Linz, = Austria
----- Original Message -----
From:=20 Jen=20 Steinberg
To: histonet@lists.utsouth w= estern.edu=20
Sent: Saturday, November 01, = 2003 10:32=20 PM
Subject: [Histonet] appropriate = pay for=20 grossing techs

Hi,
   I'm hoping someone can help me.  I was = wondering what=20 the appropriate starting wage of a grossing tech would be for a = private path=20 lab which works mainly on small skin, gyn, and oral specimens and = processes=20 about 250 cases a day (between 2 grossers).  Would the pay be any = different if the employee had an M.S.  The position is = entry-level. =20 Thanks very much. 
 
 

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Exclusive Video Premiere - Britney=20 Spears ------=_NextPart_000_001B_01C3A143.E6310980-- --__--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest From hodges420 <@t> msn.com Tue Nov 4 18:35:25 2003 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Job in Tucson Message-ID: Good evening all, We have a third position open for a histologist here in Tucson. It is with a dermpath lab Any one interested email Dr Jill Cohen jcdermpath@aol.com Thanks Tere Hodges _________________________________________________________________ Send a QuickGreet with MSN Messenger http://www.msnmessenger-download.com/tracking/cdp_games From latecor <@t> montevideo.com.uy Tue Nov 4 23:00:51 2003 From: latecor <@t> montevideo.com.uy (Carlos Defeo) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Antigen retrieval In-Reply-To: <001501c3a200$185f3be0$5a0cd2aa@unl.edu.ar> Message-ID: <5.2.1.1.0.20031105015553.00a1d980@mail.montevideo.com.uy> At 08:46 a.m. 03/11/2003 -0300, Lab Histologia wrote: >hello everyone > >i have to pepare 1 mM EDTA (pH 8.0), 100 mM Tris / 1 mM EDTA (pH 9.0) & >10 mM Tris (pH 10.0) to antigen retrieval, does anyone have a protocol >? > >thank you very much > > > >------------------------------------------------------------------- >Dr. Hugo H. Ortega (DMV, PhD) >Departament of Cellular Biology >Faculty of Veterinary Sciences >Universidad Nacional del Litoral >R.P. Kreder 2805 - Esperanza (3080) >Santa Fe - ARGENTINA >Tel. (54)3496-420639 >Fax. (54)3496-426304 >http://fcv.unl.edu.ar/histolog/ >http://fcv.unl.edu.ar/bioterio/ >Estimado Dr Ortega: Para preparar EDTA pH 8,0 tienes que disolver 0,37 grs de la sal en in litro de agua destilada,el pH resultante raramente necesita ser ajustado pues es casi exacto. La soluci?n Tris-Edta que yo uso es pH 9,5 y se elabora combinando 1,21 grs de Tris base + 0,37 grs de EDTA en un litro de agua destilada. El pH puede llevarse a 10 si lo dese?s usando NaOh 1N. Esperando te sean utiles estos datos te saluda desde Uruguay, el histotecn?logo Carlos Defeo. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/98ff5393/attachment.htm From jkiernan <@t> uwo.ca Wed Nov 5 00:10:16 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] couple of questions (Subject line) References: <20031104213846.19757.qmail@web11903.mail.yahoo.com> Message-ID: <3FA89448.8CAA1429@uwo.ca> Dear ********* You, a (? new) histonetter sent a message with the header: couple of questions Grrrr! The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. Your email asked two questions (another not good thing). For best replies, try sending two Histonet queries, with different Subject lines such as: Hoovering the cryostat safely Solvents and the VIP processor I've obscured your identity in this Histonet reply because It's not intended to be "flaming." There are lots of emails with no-good subject lines. I'm just trying to ask everyone to send a pertinent Subject line above every message. We all get lots of junk email, and we delete most of the junk messages on the basis of a few word in the Subject line. Herb and Linda are doing a SUPERB job protecting Histonet subscribers from all the viagra adverts etc. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ______________________________ She Who Shall Not be Named wrote: > > Hi Everyone, > I have a few questions, > 1. Is anyone using a "home" type vacuum for the > cryostat. Of course the bags would go in the medical > waste. > 2. Is Alcoholic formalin OK to put on the VIP tissue > processor, there is no acetic acid in the solution. > > I appreciate all your help. _______________________________________ From carl.hobbs <@t> kcl.ac.uk Wed Nov 5 04:55:12 2003 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] re dystrophin on Pwax sections References: <20031104180000.3140.42799.Mailman@swlx167.swmed.edu> Message-ID: <001101c3a38b$49728750$e8345c9f@Carlos> I have used NCL-DYS2 on pwax sections.( human and mouse were my tissues) Around 1/100-200( TCS) using streptABC pxDAB system, citric acid M/W ph6. I couldn't get 1 and 3 to work but.......it's definitely worth trying TRIS pH10 M/W. This often gives positive results when citra returns neg result carl Carl Hobbs Laboratory Manager Molecular Neurobiology Group MRC Centre For Developmental Neurobiology New Hunts House Guys Campus Kings College London SE1 1UL 020 7848 6810/fax 6816 From Terry.Marshall <@t> rothgen.nhs.uk Wed Nov 5 07:45:07 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] couple of questions (Subject line) Message-ID: Received 2 days ago: Re: [Histonet] (no subject) "You, a (? new) histonetter sent a message with the header: couple of questions Grrrr! The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. " Rremeber who sent it John:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: 05 November 2003 06:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] couple of questions (Subject line) Dear ********* You, a (? new) histonetter sent a message with the header: couple of questions Grrrr! The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. Your email asked two questions (another not good thing). For best replies, try sending two Histonet queries, with different Subject lines such as: Hoovering the cryostat safely Solvents and the VIP processor I've obscured your identity in this Histonet reply because It's not intended to be "flaming." There are lots of emails with no-good subject lines. I'm just trying to ask everyone to send a pertinent Subject line above every message. We all get lots of junk email, and we delete most of the junk messages on the basis of a few word in the Subject line. Herb and Linda are doing a SUPERB job protecting Histonet subscribers from all the viagra adverts etc. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ______________________________ She Who Shall Not be Named wrote: > > Hi Everyone, > I have a few questions, > 1. Is anyone using a "home" type vacuum for the > cryostat. Of course the bags would go in the medical > waste. > 2. Is Alcoholic formalin OK to put on the VIP tissue > processor, there is no acetic acid in the solution. > > I appreciate all your help. _______________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmondsj <@t> musc.edu Wed Nov 5 07:51:50 2003 From: edmondsj <@t> musc.edu (edmondsj) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Improved Eosin In-Reply-To: <200311041703_MC3-1-57C5-A2DC@compuserve.com> References: <200311041703_MC3-1-57C5-A2DC@compuserve.com> Message-ID: <4276404.1068022310@jbepc.thurmond-gazes.musc.edu> John, I have a Johns Hopkins eosin recipe, it is as follows: Ingredients: Eosin Y 18gms Phloxine B 7.5gms Biebrich Scarlet 1.5gms Distilled Water 2850 ml Absolute Alcohol 750 ml Procedure: 1: Dissolve all the dyes in the distilled water. 2: Add the absolute alcohol and continue stirring for at least 1 hour. This will make up a volume of 3610 ml, I add about two or three drops of glacial acetic acid to a 500 ml volume before staining tissue (this enhances the stain), you may not like the intensity but you can omit the acetic acid if you like. I hope this is helpful to you. Joyce Edmonds Research Specialist Medical University of SC Charleston, SC --On Tuesday, November 04, 2003 5:02 PM -0500 John Difford wrote: > > Dear All, > > Does anyone remember a formula for an improved Eosin solution which was > displayed on Histonet several years ago? > > It was said to have orginated at Johns Hopkins and contained some other > red dye in addition to eosin. > > I want to make up some more because it was very good for frozen sections, > but I have lost the instructions! > > Many thanks > > John Difford > Histopathology Dept > Royal Free Hospital > London > England, UK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Nov 5 08:02:27 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] couple of questions (Subject line) Message-ID: A more garbled incoherent load of rubbish is hard to imagine. Who is this T Marshall anyway? Sorry, tried to do 2 things at once. Wrong sex. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist Sent: 05 November 2003 13:45 To: jkiernan@uwo.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] couple of questions (Subject line) Received 2 days ago: Re: [Histonet] (no subject) "You, a (? new) histonetter sent a message with the header: couple of questions Grrrr! The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. " Rremeber who sent it John:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: 05 November 2003 06:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] couple of questions (Subject line) Dear ********* You, a (? new) histonetter sent a message with the header: couple of questions Grrrr! The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. Your email asked two questions (another not good thing). For best replies, try sending two Histonet queries, with different Subject lines such as: Hoovering the cryostat safely Solvents and the VIP processor I've obscured your identity in this Histonet reply because It's not intended to be "flaming." There are lots of emails with no-good subject lines. I'm just trying to ask everyone to send a pertinent Subject line above every message. We all get lots of junk email, and we delete most of the junk messages on the basis of a few word in the Subject line. Herb and Linda are doing a SUPERB job protecting Histonet subscribers from all the viagra adverts etc. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ______________________________ She Who Shall Not be Named wrote: > > Hi Everyone, > I have a few questions, > 1. Is anyone using a "home" type vacuum for the > cryostat. Of course the bags would go in the medical > waste. > 2. Is Alcoholic formalin OK to put on the VIP tissue > processor, there is no acetic acid in the solution. > > I appreciate all your help. _______________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ian.Bernard <@t> LACKLAND.AF.MIL Wed Nov 5 08:07:24 2003 From: Ian.Bernard <@t> LACKLAND.AF.MIL (Bernard Ian R SSgt 59 CRES/MSROP) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] RE: Looking for a lab to cut stents Message-ID: <588C513CC306D611A2910003479604F906591948@fsmpls17.whmc.af.mil> Cathy MAYton in Arizona Does this. -----Original Message----- From: Gordon Grant [mailto:grantgd@comcast.net] Sent: Tuesday, November 04, 2003 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Looking for a lab to cut stents I was wondering if someone out there can give me a recommendation for a lab which can provide good quality sections of implanted stents. Specifically, we have been told the FDA likes the thick saw cut, ground and polished sections rather than microtomed sections. Thanks Gordon Grant -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, November 02, 2003 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet digest, Vol 1 #113 - 2 msgs Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-admin@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. appropriate pay for grossing techs (Jen Steinberg) 2. Re: appropriate pay for grossing techs (Gudrun Lang) --__--__-- Message: 1 Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST) From: Jen Steinberg To: histonet@lists.utsouthwestern.edu Subject: [Histonet] appropriate pay for grossing techs --0-1595893577-1067722359=:57919 Content-Type: text/plain; charset=us-ascii Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. --------------------------------- Do you Yahoo!? Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919 Content-Type: text/html; charset=us-ascii
Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers).  Would the pay be any different if the employee had an M.S.  The position is entry-level.  Thanks very much. 
 
 

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Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919-- --__--__-- Message: 2 From: "Gudrun Lang" To: "Histonetliste" , "Jen Steinberg" Date: Sun, 2 Nov 2003 13:19:09 +0100 Subject: Re: [Histonet] appropriate pay for grossing techs This is a multi-part message in MIME format. ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hi Jen, I dont have an answer for you, but another question. In Austria the = person, who does crossing, is a pathologist (m.d.).=20 We have several sorts of tissue f(rom toe to sculp) and I think without = a medical uni-study, it is very difficult. Even our doctors sometimes = make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message -----=20 From: Jen Steinberg=20 To: histonet@lists.utsouthwestern.edu=20 Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the = appropriate starting wage of a grossing tech would be for a private path = lab which works mainly on small skin, gyn, and oral specimens and = processes about 250 cases a day (between 2 grossers). Would the pay be = any different if the employee had an M.S. The position is entry-level. = Thanks very much. =20 ------------------------------------------------------------------------ -= ----- Do you Yahoo!? Exclusive Video Premiere - Britney Spears ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hi Jen,
I dont have an answer for you, but = another=20 question. In Austria the person, who does crossing, is a pathologist = (m.d.).=20
We have several sorts of tissue f(rom = toe to sculp)=20 and I think without a medical uni-study, it is very difficult. Even our = doctors=20 sometimes make mistakes and look over important details.
Is it usual in USA,  that = histotechs do=20 the crossing?
 
best wishes
Gudrun Lang
general hospital Linz, = Austria
----- Original Message -----
From:=20 Jen=20 Steinberg
To: histonet@lists.utsouth w= estern.edu=20
Sent: Saturday, November 01, = 2003 10:32=20 PM
Subject: [Histonet] appropriate = pay for=20 grossing techs

Hi,
   I'm hoping someone can help me.  I was = wondering what=20 the appropriate starting wage of a grossing tech would be for a = private path=20 lab which works mainly on small skin, gyn, and oral specimens and = processes=20 about 250 cases a day (between 2 grossers).  Would the pay be any = different if the employee had an M.S.  The position is = entry-level. =20 Thanks very much. 
 
 

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Exclusive Video Premiere - Britney=20 Spears ------=_NextPart_000_001B_01C3A143.E6310980-- --__--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tiffany.L.Sheffield <@t> uth.tmc.edu Wed Nov 5 08:14:38 2003 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Tiffany L Sheffield) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] RE: Looking for a lab to cut stents References: <588C513CC306D611A2910003479604F906591948@fsmpls17.whmc.af.mil> Message-ID: <3FA905CE.BD9FCD19@uth.tmc.edu> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: Tiffany.L.Sheffield.vcf Type: text/x-vcard Size: 374 bytes Desc: Card for Tiffany Sheffield-Lopez Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/1f21ece0/Tiffany.L.Sheffield.vcf From hmcleod <@t> chempath.uct.ac.za Wed Nov 5 08:41:34 2003 From: hmcleod <@t> chempath.uct.ac.za (Mcleod) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] dystrophin Message-ID: <3FA90C1E.97F52149@chempath.uct.ac.za> Thankyou to all who replied to my dystrophin query. Much appreciated. Heather McLeod. From HornHV <@t> archildrens.org Wed Nov 5 08:45:38 2003 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] disposal of tissue Message-ID: Our disposal company requires us to empty the formalin from all the containers. We then collect the formalin and it is also taken away for disposal. I'm wondering what neutralizers anyone is using for formalin so we could dispose of it down the drain?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department. Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital From kb2drkprk <@t> yahoo.com Wed Nov 5 09:05:01 2003 From: kb2drkprk <@t> yahoo.com (Kelly Booher) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] disposal of tissue - Formalin Neutralization In-Reply-To: Message-ID: <20031105150501.63891.qmail@web20904.mail.yahoo.com> We neutralize our formalin with Sakura's Tissue-Tek Neutralex. The reaction takes approximately 15 minutes, and then the neutralized formalin is ready to go down the drain. Kelly Booher, HTL (ASCP) Anatomic Pathology Swedish Medical Center, Providence Campus Seattle, WA 98122 --- "Horn, Hazel V" wrote: > Our disposal company requires us to empty the > formalin from all the > containers. We then collect the formalin and it is > also taken away for > disposal. I'm wondering what neutralizers anyone > is using for formalin so > we could dispose of it down the drain?? > > Hazel Horn, HT/HTL (ASCP) > Histology Supervisor > Arkansas Children's Hospital > > Phone - 501.364.4240 > Fax - 501.364.3912 > > -----Original Message----- > From: Diana McCaig [mailto:dmccaig@ckha.on.ca] > Sent: Tuesday, November 04, 2003 2:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] disposal of tissue > > > How do labs dispose of pathological waste when > incineration is not available > on site. Do you have to empty each individual 90 ml > container or will > companies take them away with the fluid still in > them? I realize most > larger containers are drained and the containers > recycled but was curious to > see what is done with the smaller containers. How > long are the containers > retained and is this process done by lab staff in > the histology department. > Diana McCaig, R.T. > Charge Tech, Histology > Chatham Kent Health Alliance > 519-352-6401 (3347) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be > privileged and confidential and protected from > disclosure. If the reader of this message is not the > intended recipient, or an employee or agent > responsible for delivering this message to the > intended recipient, you are hereby notified that any > dissemination, distribution or copying of this > communication is strictly prohibited. If you have > received this communication in error, please notify > us immediately by replying to the message and > deleting it from your computer. Thank you. Arkansas > Children's Hospital > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From CCLYATT <@t> mail.mcg.edu Wed Nov 5 10:03:37 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] disposal of tissue Message-ID: FRC-5 available from S & S Co of GA (912-435-8394). Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Horn, Hazel V" 11/05/03 09:45AM >>> Our disposal company requires us to empty the formalin from all the containers. We then collect the formalin and it is also taken away for disposal. I'm wondering what neutralizers anyone is using for formalin so we could dispose of it down the drain?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department. Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Nov 5 10:15:40 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] disposal of tissue Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C67@EXCHANGE1.huntingtonhospital.com> We have a hazardous waste company that comes out and dumps all of our specimens for us. He separates the formalin from the specimens in our Morgue and takes both for disposal. He has all of the proper PPE, including a respirator. It is expensive, but I feel it is worth every penny. The time it takes to do the job and the cost of the neutralizer is expensive, too. If ventilation is not adequate and the formalin exposure is too high for the employees to perform this procedure safely, they would have to wear a respirator, and this requires formal respirator training and fitting. I know there is another company here in southern California that takes all of the specimens with the formalin still on them. I don't know if they separate them out later or just burn everything. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department. Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmuvarak <@t> facstaff.wisc.edu Wed Nov 5 10:33:05 2003 From: nmuvarak <@t> facstaff.wisc.edu (NIDAL E MUVARAK) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Somebody that can do IHC for us??? Message-ID: <42a92446e2.446e242a92@wiscmail.wisc.edu> Hi. I was wondering if there's a lab(s) out there that can do immunos for our lab. We just need help with MMP-2 and MMP-9, I got everything else to work except for these two and I'm getting frustrated. So if anyone knows of a place that provides that, I'd be very grateful if I can get some information on how to get a hold of those people. Thank you for your time. Nidal E Muvarak Associate Research Specialist Vascular Tissue Biomechanics Laboratory Department of Biomedical Engineering University of Wisconsin-Madison 1550 Engineering Dr.; Rm. 2158 Madison, WI 53706-1609 Lab: (608) 265-8921; Office: (608) 265-4205; Home: (608) 256-7934; Cell: (608) 332-6068 http://vtb.bme.wisc.edu From info <@t> instrumedics.com Wed Nov 5 10:50:10 2003 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs References: <20031105134801.5148.63736.Mailman@swlx167.swmed.edu> Message-ID: <008901c3a3bc$dffec220$6401a8c0@instrumedics1> Julian, Do you need an instrument for making the recipient block? If so most labs use the Beecher Arrayer. We market the Paraffin Tape-Transfer system that many labs find very valuable for cutting the section of cores and placing them on a slide. The tape-transfer method eliminates the water bath where cores often are lost. Please visit our web site for details www.instrumedics.com . If you need any further information please contact us. Bernice schiller@instrumedics.com From info <@t> instrumedics.com Wed Nov 5 11:00:03 2003 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs References: <20031105134801.5148.63736.Mailman@swlx167.swmed.edu> Message-ID: <00bc01c3a3be$41dca9c0$6401a8c0@instrumedics1> Eileen, The problem with using the "home" vacuum for cleaning a cryostat is that the debris collected melts and clogs the vacuum bag causing the suctioning to fail. We designed the Cryo-Vac-Away to avoid the clogging problem. The debris that is suctioned as it is generated at the block face is collected in a cold filter that is INSIDE the cryostat. The debris freeze dries and becomes very porous so that the vacuum continues to function effectively. We also have a viral/bacterial filter downstream of the primary filter that will capture pathogens so that the air that exits the system is free of toxins. Bernice schiller@instrumedics.com From Marion.Hiles <@t> north-bristol.swest.nhs.uk Wed Nov 5 10:56:27 2003 From: Marion.Hiles <@t> north-bristol.swest.nhs.uk (Marion Hiles) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] couple of questions Message-ID: <533E4A1C3061FC4EB388119D2FD7B5D40243180C@nbfexch04.north-bristol.nhs> Answer to question 2: Why would it not be OK to use alcoholic formalin since both, as separate solutions, are used in the majority of tissue processing schedules? We use alcoholic formalin on our VIP. -----Original Message----- From: eileen dusek [mailto:eileen_dusek@yahoo.com] Sent: 04 November 2003 21:39 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] couple of questions Hi Everyone, I have a few questions, 1. Is anyone using a "home" type vacuum for the cryostat. Of course the bags would go in the medical waste. 2. Is Alcoholic formalin OK to put on the VIP tissue processor, there is no acetic acid in the solution. I appreciate all your help. Eileen signature _____ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard ************************************************************************ This e-mail is confidential and privileged. If you are not the intended recipient, please accept our apologies. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents, to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. North Bristol NHS Trust does not guarantee that this material is free from viruses or any other defects, although due care has been taken to minimise the risk. No contracts may be concluded on behalf of North Bristol NHS Trust by means of email communications. Thank you for your cooperation. ************************************************************************ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/0dfe9d42/attachment.htm From tissuearray <@t> hotmail.com Wed Nov 5 11:26:35 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/d0005c02/attachment.htm From ramesh <@t> brandeis.edu Wed Nov 5 11:46:12 2003 From: ramesh <@t> brandeis.edu (Ramesh Subrahmanyam) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Distinguishing monocytes from granulocytes Message-ID: Hello Histonetters, I am trying to distinguish immature monocytes from immature granulocytes in Balb/c mice. Can anyone suggest antibodies for FACS or immunohistochemistry or RT-PCR markers that would help in this? Suggestions on other methods would be welcome too. Thanks in advance, Ramesh Brandeis University Massachusetts From michael_lafriniere <@t> memorial.org Wed Nov 5 12:18:26 2003 From: michael_lafriniere <@t> memorial.org (LaFriniere, Michael) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] seeking virtual flow information Message-ID: Histonet I would like to obtain (pro and con) information about anyone who is using US Labs "virtual flow" program or who has used it in the past. Direct response email to me would be greatly appreciated. Thank you Michael LaFriniere PA, HT (ASCP) Pathology Manager Memorial Hospital/Diagnostic Pathology Services Chattanooga TN 423-495-6117 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/b3594012/attachment.htm From gareth.davis <@t> Vanderbilt.Edu Wed Nov 5 12:29:14 2003 From: gareth.davis <@t> Vanderbilt.Edu (Davis, Gareth) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Jobs in Nashville and Bowling Green area Message-ID: <082C721AF78DB34983E8BA2CD08546216A40E9@mailbe07> Hey Histonetters, If anyone is interested in Histotechnician jobs in Nashville, Tennessee or just outside of Bowling Green, Kentucky, just e-mail me back and I'll send you the info on those. Gareth Ms. Gareth B. Davis Research Assistant II Neuro-magnetics Division of the Department of Neurology Vanderbilt University Medical Center 615-936-3318 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/78b284cd/attachment.htm From gudrun.lang <@t> aon.at Wed Nov 5 12:49:43 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] thanks for gross-answers, training in Austria Message-ID: <00ea01c3a3cd$939e15a0$0d12a8c0@SERVER> It is very interesting for me to hear about the various histo-jobs in your country. Perhaps someone wants to know, what the job training looks like in Austria. My job is called "Medical Technical Analysist" formerly "Medical Technical Assistent" (MTA) and in near future something like "Bio-Analysist" to conform to EU-line. You have to have qualification for university entrance to make the MTA-academy, but until now we do not reach a bachelor degree at the end. The school lasts three years with practical and theoretical parts. Befor your certification, you have to make a diploma project. The training comprehends all parts of laboratory work (Histo, Hematology, chemical lab, bodyfunction, molecularbiology, microbiology..) but not radiology and physical medicin. We can work in any kind of medical lab. So MTAs in Austria are really good trained and self-responsible, but have no chance to use the education for a bachelor degree and university study (yet). Within the EU there are various forms of courses, some with and some without degree. The plan is to equalize these. There is another type of labworker, the MTF, without quali for uni-entrance and self-responsibility. Our Pathologists do the University study of Humanmedicin, then work at the hospital stations (perhaps three years), then make the training for the specialist (is called assistent) at a pathology for four years. Is the histotech-training limited to histology or is there also a larger based education before? Is the Pathology Assistent a part of Med. Doctor-education or is it a special job? Perhaps someone is so kind and tells me about the training. thanks in advance Gudrun Lang general hospital, Linz, Austria -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/504d8422/attachment.htm From info <@t> instrumedics.com Wed Nov 5 13:35:19 2003 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re: Tape-Transfer for TMA References: <20031105134801.5148.63736.Mailman@swlx167.swmed.edu> Message-ID: <004201c3a3d3$f2722660$6401a8c0@instrumedics1> I think Thom's negative comments are off base. There are labs all over the world that are successfully using the PSA Tape-Transfer system. They appreciate the fact that cores are not lost in the water bath as well being able to capture the section with as many as one thousand cores on the tape with all the cores intact. Bernice schiller@instrumedics.com From TJJ <@t> Stowers-Institute.org Wed Nov 5 13:23:14 2003 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Improving morphology in cryosections of human eye Message-ID: A researcher here is trying to accomplish IHC on 10 micron sections of fresh, frozen human eye. Essentially she uses the CryoJane tape transfer system, then air-dries the slide, fixes for 15 minutes in 4% formaldehyde in PBS, washes 3 x 10 minutes in PBT, stains with DAPI 5 minutes, washes 3 x 10 minutes in PBT and coverslipped. Macroscopic evaluation of the section shows good architecture, but microscopically the cell morphology isn't good, especially the retina. The eye globes are received without the cornea, so I filled the anterior chamber of the eye with OCT, then surrounded the remainder of the globe with OCT in a Peel-away mold. I then froze it in liquid nitrogen-cooled isopentane in an attempt at minimizing freezing artifact. Any suggestions on improving our cellular morphology would be greatly appreciated. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From mfrei <@t> sial.com Wed Nov 5 13:07:56 2003 From: mfrei <@t> sial.com (Mark Frei) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Distinguishing monocytes from granulocytes Message-ID: Have you thought of cytochemistry? Chloroacetate esterase and naphthyl acetate esterase are two enzymatic assays that will easily and cheaply differentiate the mentioned cell types. Ramesh Subrahmanyam Sent by: histonet-admin@lists.utsouthwestern.edu 11/05/2003 11:46 AM To: histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Distinguishing monocytes from granulocytes Hello Histonetters, I am trying to distinguish immature monocytes from immature granulocytes in Balb/c mice. Can anyone suggest antibodies for FACS or immunohistochemistry or RT-PCR markers that would help in this? Suggestions on other methods would be welcome too. Thanks in advance, Ramesh Brandeis University Massachusetts _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/3b12bb40/attachment.htm From Joyce.Smith <@t> FLHOSP.ORG Wed Nov 5 13:27:15 2003 From: Joyce.Smith <@t> FLHOSP.ORG (Smith, Joyce (FH)) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Improved Eosin Message-ID: <7B5AC82D40B181439B4D979918017D06012E8E7C@fh2k019.fhmis.net> Does anybody know where one can purchase a Mariano S. H. di Fiore book titled "Atlas of Normal Histology"? We really need it for our students. Thanks. J.Smith Florida Hospital -----Original Message----- From: edmondsj [mailto:edmondsj@musc.edu] Sent: Wednesday, November 05, 2003 8:52 AM To: John Difford; Histonet Subject: Re: [Histonet] Improved Eosin John, I have a Johns Hopkins eosin recipe, it is as follows: Ingredients: Eosin Y 18gms Phloxine B 7.5gms Biebrich Scarlet 1.5gms Distilled Water 2850 ml Absolute Alcohol 750 ml Procedure: 1: Dissolve all the dyes in the distilled water. 2: Add the absolute alcohol and continue stirring for at least 1 hour. This will make up a volume of 3610 ml, I add about two or three drops of glacial acetic acid to a 500 ml volume before staining tissue (this enhances the stain), you may not like the intensity but you can omit the acetic acid if you like. I hope this is helpful to you. Joyce Edmonds Research Specialist Medical University of SC Charleston, SC --On Tuesday, November 04, 2003 5:02 PM -0500 John Difford wrote: > > Dear All, > > Does anyone remember a formula for an improved Eosin solution which was > displayed on Histonet several years ago? > > It was said to have orginated at Johns Hopkins and contained some other > red dye in addition to eosin. > > I want to make up some more because it was very good for frozen sections, > but I have lost the instructions! > > Many thanks > > John Difford > Histopathology Dept > Royal Free Hospital > London > England, UK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From khbarr <@t> mdanderson.org Wed Nov 5 13:33:44 2003 From: khbarr <@t> mdanderson.org (khbarr@mdanderson.org) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Positons in Houston Message-ID: The University of Texas M. D. Anderson Cancer Center in Houston, Texas currently has openings for three Histology Technicians and one Senior Histology Technician. Interested candidates should call Dianna Menard at 713-745-6184 or fax her at 713-745-7162, email dmenard@mail.mdanderson.org or apply online at www.mdanderson.org. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/3192e9de/attachment.htm From Barry.R.Rittman <@t> uth.tmc.edu Wed Nov 5 13:41:06 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Improved Eosin Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FAF3@UTHEVS3.mail.uthouston.edu> Joyce Di Fiore "An Atlas of Histology" published by Williams and Wilkins, http://www.lww.com . It is now edited by Victor P. Ereschenko ISBN 0-683-30749-5 I would first try to purchase from amazon.com as they are usually cheaper Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Smith, Joyce (FH) Sent: Wednesday, November 05, 2003 1:27 PM To: Histonet Subject: RE: [Histonet] Improved Eosin Does anybody know where one can purchase a Mariano S. H. di Fiore book titled "Atlas of Normal Histology"? We really need it for our students. Thanks. J.Smith Florida Hospital From Ronnie_Houston <@t> bshsi.com Wed Nov 5 14:04:40 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] toxoplasmosis Message-ID: <530361BF03351B4CAE5270A05D3037B5FE0502@bsrexms01.BSHSIR.COM> Is anyone able to supply a control block for Toxoplasmosis? Thanks Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From JWEEMS <@t> sjha.org Wed Nov 5 14:46:31 2003 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Specimen Retention Guidelines Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44B6D5EB@exch4.sjha.org> Help quick! I was also sited for not having the current guidelines for specimen retention in my manual. They have been changed from keeping paraffin blocks 5 years to 10 years. I have searched the CAP site and cannot locate the new documentation. Does anyone know where on the website it is? Many thanks. Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph?s Health System, Inc. From Cathy.Crumpton <@t> tuality.org Wed Nov 5 14:48:42 2003 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Blue counterstain for immunos Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031105/5d4c953c/attachment.htm From jnocito <@t> satx.rr.com Wed Nov 5 17:07:16 2003 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] disposal of tissue - Formalin Neutralization References: <20031105150501.63891.qmail@web20904.mail.yahoo.com> Message-ID: <00a001c3a3f1$a71f5480$70494542@satx.rr.com> Surgipath also has a neutralizer that works just as well. I tested the Surgipath product and obtained the same result for less money. Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas ----- Original Message ----- From: "Kelly Booher" To: "Horn, Hazel V" Cc: Sent: Wednesday, November 05, 2003 7:05 AM Subject: RE: [Histonet] disposal of tissue - Formalin Neutralization > We neutralize our formalin with Sakura's Tissue-Tek > Neutralex. The reaction takes approximately 15 > minutes, and then the neutralized formalin is ready to > go down the drain. > > Kelly Booher, HTL (ASCP) > Anatomic Pathology > Swedish Medical Center, Providence Campus > Seattle, WA 98122 > > --- "Horn, Hazel V" wrote: > > Our disposal company requires us to empty the > > formalin from all the > > containers. We then collect the formalin and it is > > also taken away for > > disposal. I'm wondering what neutralizers anyone > > is using for formalin so > > we could dispose of it down the drain?? > > > > Hazel Horn, HT/HTL (ASCP) > > Histology Supervisor > > Arkansas Children's Hospital > > > > Phone - 501.364.4240 > > Fax - 501.364.3912 > > > > -----Original Message----- > > From: Diana McCaig [mailto:dmccaig@ckha.on.ca] > > Sent: Tuesday, November 04, 2003 2:22 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] disposal of tissue > > > > > > How do labs dispose of pathological waste when > > incineration is not available > > on site. Do you have to empty each individual 90 ml > > container or will > > companies take them away with the fluid still in > > them? I realize most > > larger containers are drained and the containers > > recycled but was curious to > > see what is done with the smaller containers. How > > long are the containers > > retained and is this process done by lab staff in > > the histology department. > > Diana McCaig, R.T. > > Charge Tech, Histology > > Chatham Kent Health Alliance > > 519-352-6401 (3347) > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > The information contained in this message may be > > privileged and confidential and protected from > > disclosure. If the reader of this message is not the > > intended recipient, or an employee or agent > > responsible for delivering this message to the > > intended recipient, you are hereby notified that any > > dissemination, distribution or copying of this > > communication is strictly prohibited. If you have > > received this communication in error, please notify > > us immediately by replying to the message and > > deleting it from your computer. Thank you. Arkansas > > Children's Hospital > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > __________________________________ > Do you Yahoo!? > Protect your identity with Yahoo! Mail AddressGuard > http://antispam.yahoo.com/whatsnewfree > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adford <@t> compuserve.com Wed Nov 5 16:03:01 2003 From: adford <@t> compuserve.com (John Difford) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re: Improved Eosin Message-ID: <200311051703_MC3-1-5834-8153@compuserve.com> Dear All Many thanks to all who supplied information on The Johns Hopkins Eosin mix. Regards, John Difford Histopathology Dept Royal Free Hospital London England, UK From sebres <@t> comcast.net Wed Nov 5 17:03:14 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry References: <530361BF03351B4CAE5270A05D3037B5FE0502@bsrexms01.BSHSIR.COM> Message-ID: <000401c3a3f0$fe4f8a50$0300a8c0@homepc1> I'm teaching a neurohistology class in a research university, mainly Nissl staining, immunohistochemisty & in situ hybridization histochemistry on rat brain sections. I thought I'd add to the mix a good old fashioned enzyme histochemistry assay, such as the elegant acetylcholinesterase method described in Paxinos & Watson's Rat Brain atlas, which sounds refreshingly simple. But, to my shock, ethopropazine, since it is now used medicinally (Parsitan), seems to no longer be available except by prescription! If I understand this correctly, the main purpose of this reagent in this assay is as a cholinesterase inhibitor, in which case I'm wondering whether it might work to substitute either physostigmine, or possibly haloperidol, both of which I already have in hand? My students and I would all be extremely grateful for any advice about this! Susan Bachus, George Mason University From sebres <@t> comcast.net Wed Nov 5 17:35:50 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry References: <530361BF03351B4CAE5270A05D3037B5FE0502@bsrexms01.BSHSIR.COM> <000401c3a3f0$fe4f8a50$0300a8c0@homepc1> Message-ID: <000801c3a3f5$8bac0f50$0300a8c0@homepc1> Well, I've already dug a little deeper & come up with part of the answer, but am still in need of advice: I understand better now that it is specifically butyrylcholinesterase that ethopropazine is supposed to inhibit. I've found some evidence that ethephon is considered a specific butyrylcholinesterase inhibitor, & this is actually available for purchase from agricultural supply companies. Any thoughts out there on whether this sounds like a viable solution? Many thanks, Susan ----- Original Message ----- From: "sebres" To: "Histonet (E-mail)" Sent: Wednesday, November 05, 2003 6:03 PM Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry > I'm teaching a neurohistology class in a research university, mainly Nissl > staining, immunohistochemisty & in situ hybridization histochemistry on rat > brain sections. I thought I'd add to the mix a good old fashioned enzyme > histochemistry assay, such as the elegant acetylcholinesterase method > described in Paxinos & Watson's Rat Brain atlas, which sounds refreshingly > simple. But, to my shock, ethopropazine, since it is now used medicinally > (Parsitan), seems to no longer be available except by prescription! If I > understand this correctly, the main purpose of this reagent in this assay is > as a cholinesterase inhibitor, in which case I'm wondering whether it might > work to substitute either physostigmine, or possibly haloperidol, both of > which I already have in hand? My students and I would all be extremely > grateful for any advice about this! Susan Bachus, George Mason > University > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anissachoi <@t> hotmail.com Wed Nov 5 22:55:22 2003 From: anissachoi <@t> hotmail.com (Anissa Choi) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Wilms' Tumor WT1 Message-ID: Hi Ann, We use mesothelioma as control for WT1. As it is hard to get mesothelioma, I have tried Testis which give nice nuclear staining. When we run out of mesothelioma tissue, we will use testis. Anissa Choi Anatomical Pathology Burnaby Hospital Canada >From: "ANN MARUSKA" >To: >Subject: [Histonet] Wilms' Tumor WT1 >Date: Wed, 22 Oct 2003 15:43:41 -0500 > >Hi Histonetters, > >I am working up Wilms' Tumor (WT1) - and wondering what other labs are >using for control. Unfortunately, a Wilms tumor is not an option for >me. Anyone using a mesothelioma or some other tissue for a control with >this antibody? >Thanks in advance. > >Ann > >Ann Maruska >Fairview-University Medical Center >Mpls. MN 55454 >amarusk1@fairview.org >612-273-9119 ><< ANNMARUSKA.vcf >> _________________________________________________________________ MSN 8 helps eliminate e-mail viruses. Get 2 months FREE*. http://join.msn.com/?page=features/virus&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From anissachoi <@t> hotmail.com Thu Nov 6 01:34:43 2003 From: anissachoi <@t> hotmail.com (Anissa Choi) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re:Modified ZN (Wade Fite) Message-ID: I would fix cytology smears in Formalin for Modified ZN. If smears come fixed in alcohol, should I do the stain? A question was forwarded to me : Tissue go through alcohol during processing.Is it really critical to avoid alcohol when taking tissue sections down to water for this special stain? Thanks in advance for your input. Anissa Choi Anatomical Pathology Burnaby Hospital Canada _________________________________________________________________ Tired of spam? Get advanced junk mail protection with MSN 8. http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From manuelle.debock <@t> UGent.be Thu Nov 6 04:36:03 2003 From: manuelle.debock <@t> UGent.be (Manuelle De Bock) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] CD 4/CD 8 staining of frozen stomach sections of mice Message-ID: <001201c3a451$c7568bf0$ed54bec1@rug.ac.be> Hello everyone, I am trying (for a long time allready) to produce a good CD4/CD8 staining of frozen stomach tissue (mice). We use the Dako rat anti-mouse CD4 antibody but always encounter a large amound of background after staining (it seems like there is a lot of cross reactivity with the glandular epithelium). Can anyone help me to solve this problem ? Thanx ! Manuelle De Bock, dierenarts (DVM) Laboratory of Veterinary Pathology Department of Pathology, Bacteriology and Avian Diseases Faculty of Veterinary Medicine Ghent University Salisburylaan 133 B9820 Merelbeke - Belgium Tel 0032(0)9 264 7745 Fax 0032(0)9 264 7789 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/faee1bf2/attachment.htm From tissuearray <@t> hotmail.com Thu Nov 6 07:26:03 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Tape-Transfer System - Tissue Microarray Poll Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/5e7b65f4/attachment.htm From ccrowder <@t> mail.vetmed.lsu.edu Thu Nov 6 07:47:29 2003 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Genetics anyone? Message-ID: Good morning - This questions was put to me yesterday and, knowing little about genetics, I'm stumped. Can any of you help me. This researcher is collecting samples which he says are either diploid, triploid or tetraploid. He states that someone told him there is a stain techniques that "would distinguish the 'ploidy' by the intensity of the stained tissue". Have any of you heard of such a thing or know someone I can contact for this "unusual" question? Thank you, in advance. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 From brett_connolly <@t> merck.com Thu Nov 6 08:21:53 2003 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Genetics anyone? Message-ID: Cheryl, He wants a Feulgen stain. Kits are available... take a look at the Scytek or Chromavision websites. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com -----Original Message----- From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu] Sent: Thursday, November 06, 2003 8:47 AM To: Histonet Subject: [Histonet] Genetics anyone? Good morning - This questions was put to me yesterday and, knowing little about genetics, I'm stumped. Can any of you help me. This researcher is collecting samples which he says are either diploid, triploid or tetraploid. He states that someone told him there is a stain techniques that "would distinguish the 'ploidy' by the intensity of the stained tissue". Have any of you heard of such a thing or know someone I can contact for this "unusual" question? Thank you, in advance. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peoshel <@t> wisc.edu Thu Nov 6 08:43:25 2003 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Genetics anyone? In-Reply-To: References: Message-ID: Cheryl, Periodic acid-Schiff's stains nucleic acids stochiometrically, allowing ploidy determination by microspectrophotometry. Botanists used to do this in the 60s and 70s before the molecular types killed microscopy. The light 'scope I learned this one was an early 60s vintage, including the spec. Check around the botanists at LSU -- the older ones -- and see if any of them remember this. Ploidy is particularly important in botany. Phil >Good morning - This questions was put to me yesterday and, knowing >little about genetics, I'm stumped. Can any of you help me. This >researcher is collecting samples which he says are either diploid, >triploid or tetraploid. He states that someone told him there is a >stain techniques that "would distinguish the 'ploidy' by the intensity >of the stained tissue". > >Have any of you heard of such a thing or know someone I can contact for >this "unusual" question? Thank you, in advance. Cheryl > > >Cheryl Crowder, BA, HTL(ASCP) >Chief Technologist >Anatomic Pathology >Department of Pathobiological Sciences >School of Veterinary Medicine >Louisiana State University >Skip Bertman Drive >Baton Rouge, LA 70803 > >225-578-9734 >FAX: 225-578-9720 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From histosci <@t> shentel.net Thu Nov 6 08:56:39 2003 From: histosci <@t> shentel.net (HSRL) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Leitz 1512 manual Message-ID: <000701c3a476$33bd5c50$0200a8c0@HSRLMAIN> Dear Netters, Does anyone happen to have a Leitz 1512 manual lying around that you no longer need? If so we would be interested in buying it from you. Have a great day. Beth Poole HSRL- A GLP Compliant Laboratory 137 South Main Street Woodstock, Virginia 22664 (540)459-8211 Fax: (540)459-8217 beth@hsrl.org www.hsrl.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/2770edc0/attachment.htm From peoshel <@t> wisc.edu Thu Nov 6 08:56:32 2003 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Genetics anyone? In-Reply-To: References: Message-ID: )#&$()*&$ What I get emailing without looking at my references. Feulgen's, yes, not periodic acid-Schiff's, although Feulgen's does use Schiff's. A good reference is Berlyn and Miksche "Botanical Microtechnique and Cytochemistry", Chap. 11, pp. 246ff One of the classic methods. Phil >Cheryl, >He wants a Feulgen stain. Kits are available... take a look at the Scytek or >Chromavision websites. > >Brett > >Brett M. Connolly, Ph.D. >Merck & Co., Inc. >MRL, Imaging Research >WP26A-3000 >PO Box 4 >West Point, PA 19486 >PH 215-652-2501 >fax. 215-652-2075 >e-mail. brett_connolly@merck.com > > >-----Original Message----- >From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu] >Sent: Thursday, November 06, 2003 8:47 AM >To: Histonet >Subject: [Histonet] Genetics anyone? > > >Good morning - This questions was put to me yesterday and, knowing >little about genetics, I'm stumped. Can any of you help me. This >researcher is collecting samples which he says are either diploid, >triploid or tetraploid. He states that someone told him there is a >stain techniques that "would distinguish the 'ploidy' by the intensity >of the stained tissue". > >Have any of you heard of such a thing or know someone I can contact for >this "unusual" question? Thank you, in advance. Cheryl > > >Cheryl Crowder, BA, HTL(ASCP) >Chief Technologist >Anatomic Pathology >Department of Pathobiological Sciences >School of Veterinary Medicine >Louisiana State University >Skip Bertman Drive >Baton Rouge, LA 70803 > >225-578-9734 >FAX: 225-578-9720 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From sebres <@t> comcast.net Thu Nov 6 08:58:33 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry References: <530361BF03351B4CAE5270A05D3037B5FE0504@bsrexms01.BSHSIR.COM> Message-ID: <001101c3a476$73894ce0$0300a8c0@homepc1> Bless you! Thank goodness, and thank YOU (& Histonet!) for not having to reinvent the wheel! I'm very grateful to be able to benefit from your experience! with my sincere appreciation, Susan ----- Original Message ----- From: "Houston, Ronnie" To: "'sebres'" Sent: Thursday, November 06, 2003 8:12 AM Subject: RE: [Histonet] ethopropazine/acetylcholinesterase histochemistry > > Susan, > > We used to use iso-OMPA (can't remember it's full name now) to inhibit > non-specific esterases when I worked in neuropathology many moons ago. > However, by its very nature (an extremely potent cholinesterase inhibitor) > we decided to try it without the inhibitor present. Almost impossible to > notice the difference!!! Personally, with the inherent risks involved, I > would do without. > > Ronnie > > Ronnie Houston > Regional Histology Operations Manager > Bon Secours HealthPartners Laboratories > 5801 Bremo Road > Richmond, VA 23226 > (804) 287 7972 > ronnie_houston@bshsi.com > > > -----Original Message----- > From: sebres [mailto:sebres@comcast.net] > Sent: Wednesday, November 05, 2003 6:36 PM > To: Histonet (E-mail) > Subject: Re: [Histonet] ethopropazine/acetylcholinesterase > histochemistry > > > Well, I've already dug a little deeper & come up with part of the answer, > but am still in need of advice: I understand better now that it is > specifically butyrylcholinesterase that ethopropazine is supposed to > inhibit. I've found some evidence that ethephon is considered a specific > butyrylcholinesterase inhibitor, & this is actually available for purchase > from agricultural supply companies. Any thoughts out there on whether this > sounds like a viable solution? Many thanks, Susan > ----- Original Message ----- > From: "sebres" > To: "Histonet (E-mail)" > Sent: Wednesday, November 05, 2003 6:03 PM > Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry > > > > I'm teaching a neurohistology class in a research university, mainly Nissl > > staining, immunohistochemisty & in situ hybridization histochemistry on > rat > > brain sections. I thought I'd add to the mix a good old fashioned enzyme > > histochemistry assay, such as the elegant acetylcholinesterase method > > described in Paxinos & Watson's Rat Brain atlas, which sounds refreshingly > > simple. But, to my shock, ethopropazine, since it is now used medicinally > > (Parsitan), seems to no longer be available except by prescription! If I > > understand this correctly, the main purpose of this reagent in this assay > is > > as a cholinesterase inhibitor, in which case I'm wondering whether it > might > > work to substitute either physostigmine, or possibly haloperidol, both of > > which I already have in hand? My students and I would all be extremely > > grateful for any advice about this! Susan Bachus, George Mason > > University > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ____________________________________________________________________________ ____________________________________________________ > ____________________________________________________________________________ ____________________________________________________ > > The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. > It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. > In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information > contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should > this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, > and permanently delete the original e-mail, attachment(s), and any copies. From pkubier <@t> hotmail.com Thu Nov 6 09:30:50 2003 From: pkubier <@t> hotmail.com (Patty Kubier) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] DHT antibody Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/709f7b75/attachment.htm From tissuearray <@t> hotmail.com Thu Nov 6 09:48:17 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:09 2005 Subject: Fwd: Re: [Histonet] Tape-Transfer System - Tissue Microarray Poll Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/86a77e2d/attachment.htm From Michele.Ellender <@t> nrpb.org Thu Nov 6 09:54:33 2003 From: Michele.Ellender <@t> nrpb.org (Michele Ellender) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] DNA extraction from wax sections Message-ID: Dear all, Does anyone out there have a protocol to extract DNA from sections of formalin fixed wax embedded mouse tissues? I've been asked to microdissect GI tumours from wax sections and extract the DNA for use in PCR techniques. Hope someone can help. Many thanks, Michele Dr Michele Ellender Radiation Effects Department, NRPB, Chilton, Didcot, Oxon. OX11 0RQ. UK michele.ellender@nrpb.org This e-mail transmission is strictly confidential and intended solely for the person or organisation to whom it is addressed. It may contain privileged and confidential information and if you are not the intended recipient, please do not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, please notify the sender as soon as possible and delete the message. Please note that NRPB monitors incoming and outgoing e-mail for compliance with its Acceptable Use Policy. This will include scanning incoming e-mails to detect viruses and key-words and may in some circumstances result in the manual monitoring of the content of messages. National Radiological Protection Board E-mail: nrpb@nrpb.org Web site: www.nrpb.org -------------------------------------------- From mari.ann.mailhiot <@t> leica-microsystems.com Thu Nov 6 10:05:20 2003 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Leitz 1512 manual Message-ID: Beth Give me a call and I will send you a copy of the Leitz 1512 manual. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "HSRL" To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] Leitz 1512 manual western.edu 11/06/2003 08:56 AM Please respond to histosci Dear Netters, Does anyone happen to have a Leitz 1512 manual lying around that you no longer need?? If so we would be interested in buying it from you.? Have a great day. Beth Poole HSRL- A GLP Compliant Laboratory 137 South Main Street Woodstock, Virginia? 22664 (540)459-8211 Fax: (540)459-8217 beth@hsrl.org www.hsrl.org ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ From tpmorken <@t> labvision.com Thu Nov 6 10:20:05 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Genetics anyone? Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CCB9@usca0082k03.rallansci.apogent.com> Cheryl, As noted by others, ploidy can be determined using the feulgen stain. While a simple bright-field examination can show differences in stain intensity related to ploidy that would be a very rough guess as to actual ploidy differences. More exact analysis requires either an image analysis system (several on the market) or flow cytometry. Here is a concise explanation of one method of ploidy analysis: http://www.fairimag.co.uk/fairfield/dna-cytometry/ploidy-feulgen.html Tim Morken Lab Vision / NeoMarkers www.labvision.com -----Original Message----- From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu] Sent: Thursday, November 06, 2003 5:47 AM To: Histonet Subject: [Histonet] Genetics anyone? Good morning - This questions was put to me yesterday and, knowing little about genetics, I'm stumped. Can any of you help me. This researcher is collecting samples which he says are either diploid, triploid or tetraploid. He states that someone told him there is a stain techniques that "would distinguish the 'ploidy' by the intensity of the stained tissue". Have any of you heard of such a thing or know someone I can contact for this "unusual" question? Thank you, in advance. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Thu Nov 6 10:26:06 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry Message-ID: <494304423C63E246A5CF87A3AEEB577011B5A8@bumail01.barrynet.barry.edu> Back in the dark ages, we used diisopropylfluorophosphate as a cholinesterase inhibitor. It's very effective (and very expensive), but not very specific: it also inhibits trypsin, thrombin, and plasmin. Diisopropylfluorophosphate is still available from SIGMA (1-800-325-3010), cat # D 0879 @ 227.65 / g. Ethopropazine is still in the current SIGMA catalog, cat # E 2880 @ 16.60 / 5 g. -----Original Message----- From: sebres [mailto:sebres@comcast.net] Sent: Wednesday, November 05, 2003 6:36 PM To: Histonet (E-mail) Subject: Re: [Histonet] ethopropazine/acetylcholinesterase histochemistry Well, I've already dug a little deeper & come up with part of the answer, but am still in need of advice: I understand better now that it is specifically butyrylcholinesterase that ethopropazine is supposed to inhibit. I've found some evidence that ethephon is considered a specific butyrylcholinesterase inhibitor, & this is actually available for purchase from agricultural supply companies. Any thoughts out there on whether this sounds like a viable solution? Many thanks, Susan ----- Original Message ----- From: "sebres" To: "Histonet (E-mail)" Sent: Wednesday, November 05, 2003 6:03 PM Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry > I'm teaching a neurohistology class in a research university, mainly > Nissl staining, immunohistochemisty & in situ hybridization > histochemistry on rat > brain sections. I thought I'd add to the mix a good old fashioned > enzyme histochemistry assay, such as the elegant acetylcholinesterase > method described in Paxinos & Watson's Rat Brain atlas, which sounds > refreshingly simple. But, to my shock, ethopropazine, since it is now > used medicinally (Parsitan), seems to no longer be available except by > prescription! If I understand this correctly, the main purpose of > this reagent in this assay is > as a cholinesterase inhibitor, in which case I'm wondering whether it might > work to substitute either physostigmine, or possibly haloperidol, both > of which I already have in hand? My students and I would all be extremely > grateful for any advice about this! Susan Bachus, George Mason > University > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From sebres <@t> comcast.net Thu Nov 6 10:34:41 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry References: <494304423C63E246A5CF87A3AEEB577011B5A8@bumail01.barrynet.barry.edu> Message-ID: <000a01c3a483$e0c0ab20$0300a8c0@homepc1> Thanks much! I spoke on the phone with Sigma before posting my question & was told by them that they no longer carry the ethopropazine, nor did they have any advice on a substitute for me. Based on Ronnie's experience it doesn't sound worth sinking a lot of $ into this, but as luck would have it, my husband, a patchclamper, has some iso-OMPA that I can use. Histonet is the greatest!--one of our posters at Society for Neuroscience this weekend is on teaching histology in a univ psych dept (in the "teaching neuroscience" posters) & I hope you folks don't mind if I include a little PR for Histonet on it! gratefully, Susan ----- Original Message ----- From: "Smith, Allen" To: "sebres" Cc: Sent: Thursday, November 06, 2003 11:26 AM Subject: RE: [Histonet] ethopropazine/acetylcholinesterase histochemistry Back in the dark ages, we used diisopropylfluorophosphate as a cholinesterase inhibitor. It's very effective (and very expensive), but not very specific: it also inhibits trypsin, thrombin, and plasmin. Diisopropylfluorophosphate is still available from SIGMA (1-800-325-3010), cat # D 0879 @ 227.65 / g. Ethopropazine is still in the current SIGMA catalog, cat # E 2880 @ 16.60 / 5 g. -----Original Message----- From: sebres [mailto:sebres@comcast.net] Sent: Wednesday, November 05, 2003 6:36 PM To: Histonet (E-mail) Subject: Re: [Histonet] ethopropazine/acetylcholinesterase histochemistry Well, I've already dug a little deeper & come up with part of the answer, but am still in need of advice: I understand better now that it is specifically butyrylcholinesterase that ethopropazine is supposed to inhibit. I've found some evidence that ethephon is considered a specific butyrylcholinesterase inhibitor, & this is actually available for purchase from agricultural supply companies. Any thoughts out there on whether this sounds like a viable solution? Many thanks, Susan ----- Original Message ----- From: "sebres" To: "Histonet (E-mail)" Sent: Wednesday, November 05, 2003 6:03 PM Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry > I'm teaching a neurohistology class in a research university, mainly > Nissl staining, immunohistochemisty & in situ hybridization > histochemistry on rat > brain sections. I thought I'd add to the mix a good old fashioned > enzyme histochemistry assay, such as the elegant acetylcholinesterase > method described in Paxinos & Watson's Rat Brain atlas, which sounds > refreshingly simple. But, to my shock, ethopropazine, since it is now > used medicinally (Parsitan), seems to no longer be available except by > prescription! If I understand this correctly, the main purpose of > this reagent in this assay is > as a cholinesterase inhibitor, in which case I'm wondering whether it might > work to substitute either physostigmine, or possibly haloperidol, both > of which I already have in hand? My students and I would all be extremely > grateful for any advice about this! Susan Bachus, George Mason > University > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From mward <@t> wfubmc.edu Thu Nov 6 11:49:36 2003 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] FITC-IgG Message-ID: <61135F0455D33347B5AAE209B903A304032622F9@EXCHVS2.medctr.ad.wfubmc.edu> I am in search of a new vendor for our FITC-IgG that we use for the direct immuno staining for renal and skin biopsies. Our current vendor, BioWhittaker, informed me that their antibody is on back order until mid-January. Any help I can get would be greatly appreciated. Thanks! Martha Ward Wake Forest University Baptist Medical Center -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/23e4022c/attachment.htm From kspencer <@t> utmem.edu Thu Nov 6 12:01:47 2003 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] DNA extraction from wax sections In-Reply-To: Message-ID: <49AB8898-1083-11D8-9CFC-000393967904@utmem.edu> I have a DNA extraction protocol for use with laser capture microscopy. Would you like a copy? Kathleen Spencer Lab Manager/LCM Supervisor Pharmacology UTHSC Memphis, TN On Thursday, November 6, 2003, at 09:54 AM, Michele Ellender wrote: > Dear all, > Does anyone out there have a protocol to extract DNA from sections of > formalin fixed wax embedded mouse tissues? > I've been asked to microdissect GI tumours from wax sections and > extract the > DNA for use in PCR techniques. > Hope someone can help. > Many thanks, > Michele > > Dr Michele Ellender > Radiation Effects Department, > NRPB, Chilton, Didcot, > Oxon. OX11 0RQ. UK > > michele.ellender@nrpb.org > > > > This e-mail transmission is strictly confidential and intended > solely for the person or organisation to whom it is > addressed. It may contain privileged and confidential > information and if you are not the intended recipient, > please do not copy, distribute or take any action in > reliance on it. If you have received this e-mail in error, > please notify the sender as soon as possible and delete > the message. > > Please note that NRPB monitors incoming and outgoing > e-mail for compliance with its Acceptable Use Policy. This > will include scanning incoming e-mails to detect viruses > and key-words and may in some circumstances result in > the manual monitoring of the content of messages. > > National Radiological Protection Board > E-mail: nrpb@nrpb.org > Web site: www.nrpb.org > -------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> earthlink.net Thu Nov 6 12:04:21 2003 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] CD4/CD8 In-Reply-To: <20031106163500.2128.98916.Mailman@swlx167.swmed.edu> References: <20031106163500.2128.98916.Mailman@swlx167.swmed.edu> Message-ID: <6.0.0.22.0.20031106125401.02998ca0@127.0.0.1> Manuelle, I would certainly recommend using Envision (or some non-biotin based detection system). Both antibodies work best using high pH retrieval which tends to necessitate Envision. I can't speak for Dako CD4 as we use Novocastra but we get much better results doing the peroxidase block prior to antigen retrieval. The H2O2 interferes with the signal. It sounds like a pain but the results are clearly better. good luck, Amos Brooks At 11:35 AM 11/6/03, you wrote: >Message: 19 >Reply-To: "Manuelle De Bock" >From: "Manuelle De Bock" >To: >Date: Thu, 6 Nov 2003 11:36:03 +0100 >Subject: [Histonet] CD 4/CD 8 staining of frozen stomach sections of mice > >This is a multi-part message in MIME format. > >------=_NextPart_000_000F_01C3A45A.2907E120 >Content-Type: text/plain; > charset="iso-8859-1" >Content-Transfer-Encoding: quoted-printable > >Hello everyone, > >I am trying (for a long time allready) to produce a good CD4/CD8 = >staining of frozen stomach tissue (mice). We use the Dako rat anti-mouse = >CD4 antibody but always encounter a large amound of background after = >staining (it seems like there is a lot of cross reactivity with the = >glandular epithelium). Can anyone help me to solve this problem ? >Thanx ! > > >Manuelle De Bock, dierenarts (DVM) >Laboratory of Veterinary Pathology=20 >Department of Pathology, Bacteriology and Avian Diseases >Faculty of Veterinary Medicine >Ghent University >Salisburylaan 133 >B9820 Merelbeke - Belgium >Tel 0032(0)9 264 7745 >Fax 0032(0)9 264 7789 From DCoulter <@t> Lifespan.org Thu Nov 6 12:20:15 2003 From: DCoulter <@t> Lifespan.org (Coulter, Diane) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Sliding microtomes Message-ID: Our research department is interested in purchasing a sliding microtome for large sections. Does anyone out there have any suggestions about manufacturers, vendors, etc.? Thanks Diane Coulter Rhode island Hospital From tvedilago <@t> system1.net Thu Nov 6 12:29:37 2003 From: tvedilago <@t> system1.net (Tommy Vedilago) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Histology positions Message-ID: Hello Histonetters, Just wanted to drop an email on the Histonet and let you know what we are working on. We still need a supervisor for Philadelphia, Tampa, NYC, and now an AP Manager for Washington D.C. We are also looking for techs in Tucson, Ft Lauderdale, Nashville, and Michigan. I appreciate the response I've received from the Histology community and look forward to working with as many of you as possible. Please feel free to call me at 866-797-8361. Tommy Vedilago System 1 Search (864) 627-0012 (864) 627-0013 Fax From japoteete <@t> saintfrancis.com Thu Nov 6 12:22:22 2003 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] FITC-IgG Message-ID: We use #311 from PROTOS. Their telephone number is 650-652-3450 in Burlingame, CA. Jacquie Poteete MT(ASCP), Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com > -----Original Message----- > From: Martha Ward [SMTP:mward@wfubmc.edu] > Sent: Thursday, November 06, 2003 11:50 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FITC-IgG > > I am in search of a new vendor for our FITC-IgG that we use for the > direct immuno staining for renal and skin biopsies. Our current vendor, > BioWhittaker, informed me that their antibody is on back order until > mid-January. Any help I can get would be greatly appreciated. Thanks! > > Martha Ward > Wake Forest University Baptist Medical Center > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From gudrun.lang <@t> aon.at Thu Nov 6 12:36:25 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:09 2005 Subject: Fw: [Histonet] Genetics anyone? Message-ID: <005501c3a494$e26857f0$0d12a8c0@SERVER> > Is it possible, that the researcher means FISH? > As far as I know, DNA Sequences are shown. And when the sequence occurs more > than normally two times (diploid) you get more fluoreszenz signals. > I dont perform FISH. So my information is not really detailled. > Gundi Lang > > > ----- Original Message ----- > From: "Cheryl Crowder" > To: "Histonet" > Sent: Thursday, November 06, 2003 2:47 PM > Subject: [Histonet] Genetics anyone? > > > > Good morning - This questions was put to me yesterday and, knowing > > little about genetics, I'm stumped. Can any of you help me. This > > researcher is collecting samples which he says are either diploid, > > triploid or tetraploid. He states that someone told him there is a > > stain techniques that "would distinguish the 'ploidy' by the intensity > > of the stained tissue". > > > > Have any of you heard of such a thing or know someone I can contact for > > this "unusual" question? Thank you, in advance. Cheryl > > > > > > Cheryl Crowder, BA, HTL(ASCP) > > Chief Technologist > > Anatomic Pathology > > Department of Pathobiological Sciences > > School of Veterinary Medicine > > Louisiana State University > > Skip Bertman Drive > > Baton Rouge, LA 70803 > > > > 225-578-9734 > > FAX: 225-578-9720 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From gareth.davis <@t> Vanderbilt.Edu Thu Nov 6 12:38:42 2003 From: gareth.davis <@t> Vanderbilt.Edu (Davis, Gareth) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Sliding microtomes Message-ID: <082C721AF78DB34983E8BA2CD08546216A40F1@mailbe07> We use Microm Sliding Microtome, which can be purchased from Richard-Allen Scientific. They have automated and manual versions. They have never given us a problem. Gareth Ms. Gareth B. Davis Research Assistant II Neuro-magnetics Division of the Department of Neurology Vanderbilt University Medical Center 615-936-3318 -----Original Message----- From: Coulter, Diane [mailto:DCoulter@Lifespan.org] Sent: Thu 11/6/2003 12:20 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Subject: [Histonet] Sliding microtomes Our research department is interested in purchasing a sliding microtome for large sections. Does anyone out there have any suggestions about manufacturers, vendors, etc.? Thanks Diane Coulter Rhode island Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/90418e0e/attachment.htm From t-sherman <@t> comcast.net Thu Nov 6 12:44:52 2003 From: t-sherman <@t> comcast.net (Todd Sherman) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] Re: Genetics anyone? In-Reply-To: <20031106163500.2128.98916.Mailman@swlx167.swmed.edu> References: <20031106163500.2128.98916.Mailman@swlx167.swmed.edu> Message-ID: On Thu, 06 Nov 2003 10:35:00 -0600, wrote: Hello Cheryl, 'Ploidy' is the numeric state of the chromosomes, so to speak: If n = species specific number of chromosomes in a given somatic cell, then Haploid - n ; single set of unpaired chromosomes Diploid - 2n ; two of each chromosome (a pair of chromosomes) and the standard state of a typical, "resting" cell Triploid - 3n Tetraploid - 4n So, in Homo sapiens, haploid(23), diploid(46), triploid(69), tetraploid(92) are the amounts of chromosomal material - ie. number of chromosomes specifically and amount of chromatin generically. I do not know about the stain to which the researcher alludes but I suspect it is able to differentiate between the cell's nuclear condition by the amount of chromatin that is visualized after staining. Having said that, unless the differences are dramatic and obvious, the use of the word "intensity" scares me. My expertise, such that it is, was in immunohistochemistry... determining intensity was often an error-prone exercise. Unless you are able to stain a monolayer and incorporate the entire nucleus of the cells in your evaluations, this relative intensity stuff is going to be difficult. If you section a tissue block and include the entire nucleus of a diploid cell and half the nucleus of a tetraploid cell, the amount of nuclear chromatin will be the same. Unless there is some other differentiating feature between cell stages to help you in your analysis, these relative comparisons will be hard... and I just pointed out the most extreme case. What if you have 1/3 of one cell's nucleus and 2/3 of another cell's nucleus? And what if the first cell is tetraploid (1/3 x 4n = 4/3n) and the second is triploid (2/3 x 3n = 6/3n)? In this case, the triploid cell would appear to have more chromatin than the tetraploid cell. Moreover, how would you know which cell had the higher ploidy if only part of the nucleus is visible? Now, if you are looking at entire tissues at low magnification rather than specific cells, then the alluded stain might be appropriate. That is because the ploidy is likely uniform/homogeneous throughout the tissue and localized aberrations are insignicant or absent. This example would occur in an experiment where Tissue A (normal diploid) and Tissue B (abnormal n-ploid) were compared. If Tissue B stained twice as intensely as tissue A, you might "conclude" that Tissue B had its nuclear material changed to tetraploid during experimentation. The researcher should probably give you more specifics about his study and the stain if possible. I probably gave you more info than you wanted but I do not think these concerns can be overlooked. Good luck, Todd Todd Sherman President HistoSoft Corporation > 21. Genetics anyone? (Cheryl Crowder) > --__--__-- > > Message: 21 > Date: Thu, 06 Nov 2003 07:47:29 -0600 > From: "Cheryl Crowder" > To: "Histonet" > Subject: [Histonet] Genetics anyone? > > Good morning - This questions was put to me yesterday and, knowing > little about genetics, I'm stumped. Can any of you help me. This > researcher is collecting samples which he says are either diploid, > triploid or tetraploid. He states that someone told him there is a > stain techniques that "would distinguish the 'ploidy' by the intensity > of the stained tissue". > > Have any of you heard of such a thing or know someone I can contact for > this "unusual" question? Thank you, in advance. Cheryl > > > Cheryl Crowder, BA, HTL(ASCP) > Chief Technologist > Anatomic Pathology > Department of Pathobiological Sciences > School of Veterinary Medicine > Louisiana State University > Skip Bertman Drive > Baton Rouge, LA ?70803 > > 225-578-9734 > FAX: ?225-578-9720 > > --__--__-- -- >>>>>>>> www.histosoft.com <<<<<<<< <<<<<<<< Biology In A New Form (c) >>>>>>> From GDawson <@t> Milw.Dynacare.com Thu Nov 6 13:02:54 2003 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:22:09 2005 Subject: [Histonet] FITC IgG Message-ID: Martha, I use DAKO FITC IgG here and it works very well. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI From alex.mclellan <@t> stonebow.otago.ac.nz Thu Nov 6 13:11:38 2003 From: alex.mclellan <@t> stonebow.otago.ac.nz (alex.mclellan@stonebow.otago.ac.nz) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] staining mouse tissues using mouse monoclonals Message-ID: <3FAB53BA.13880.170E46@localhost> Reply to Alan Meeker (10/28/03): staining mouse tissues using mouse monoclonals Dear Alan, The best fix will be to biotinylate your monoclonal (or purchase a biotinylated) and use labelled streptavidin to detect the signal. This would prevent reaction with Ig+ B cells in the tissue. However, if your reagent is too sparse and expensive to do an in-house biotinylation, I would perform the following slightly more complicated routine: First block the endogenous mouse Ig on the tissue with a Jackson anti-mouse Ig Fab Ab (donkey or goat would be cleanest) and wash away excess. A Fab Ab is the key here to avoid later reaction against your monoclonal, as would happen with a divalent Ig (eg. whole or Fab)2 molecule. Then react the section with your monoclonal and use a labelled anti-mouse Ab to detect. I have had a lot of luck using Jackson Ab (preferably donkey), one as Fab form (to block) and the other as whole or (Fab)2 to detect (labelled with HRP). The detection could also be, but need not, be a Fab form. The specificities and affinities of the blocker and detection Ab (against mouse Ig) should be as similar as possible for obvious reasons, so try to choose from the same company (who may also be able to advise on the best choice). -- A.D. McLellan Department of Microbiology University of Otago PO Box 56 Dunedin New Zealand Tel: +64 3 479 7728, Lab: +64 3 479 7147 Fax: +64 3 479 8540 http://microbes.otago.ac.nz/dept/index.html From pruegg <@t> colobio.com Thu Nov 6 13:24:01 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Distinguishing monocytes from granulocytes In-Reply-To: Message-ID: I have done some promising work on mouse tissue using the anti-human DAKO CD68 PGM1 clone with a mouse on mouse biotinylation detection system. The PGM1 clone MO876 labels macrophages and monocytes but not other myeloid cells. This may be useful for your work. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Ramesh Subrahmanyam Sent: Wednesday, November 05, 2003 10:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Distinguishing monocytes from granulocytes Hello Histonetters, I am trying to distinguish immature monocytes from immature granulocytes in Balb/c mice. Can anyone suggest antibodies for FACS or immunohistochemistry or RT-PCR markers that would help in this? Suggestions on other methods would be welcome too. Thanks in advance, Ramesh Brandeis University Massachusetts _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JosefaNava <@t> texashealth.org Thu Nov 6 13:23:46 2003 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] LOOKING FOR ANTIBODY - alpha-methylacyl-CoA Racemase (AMACR) Message-ID: <3B1A15523787D71197170000E867C0A20120AF8C@PHDEX04> Hello to Everyone, I need help please where to order alpha methyl-CoA Racemase (AMACR). Thank you so much. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From la.sebree <@t> hosp.wisc.edu Thu Nov 6 13:32:13 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] LOOKING FOR ANTIBODY - alpha-methylacyl-CoA Racemase (AMACR) Message-ID: Try Biocare Medical for P504S, (800)799-9499. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Nava, Josefa [mailto:JosefaNava@texashealth.org] Sent: Thursday, November 06, 2003 1:24 PM To: 'HISTONET@PATHOLOGY.SWMED.EDU' Subject: [Histonet] LOOKING FOR ANTIBODY - alpha-methylacyl-CoA Racemase (AMACR) Hello to Everyone, I need help please where to order alpha methyl-CoA Racemase (AMACR). Thank you so much. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sa.drew <@t> hosp.wisc.edu Thu Nov 6 13:39:00 2003 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] LOOKING FOR ANTIBODY - alpha-methylacyl-CoA Racemase (AMACR) Message-ID: We use the rabbit monoclonal from BioCare Medical for use on the Ventana stainers. Cat #VP200G Sally Ann Drew-MT(ASCP) Univ. of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. VAH-DM223 Madison, WI 53792-2472 608-265-6596 sa.drew@hosp.wisc.edu -----Original Message----- From: Nava, Josefa [mailto:JosefaNava@texashealth.org] Sent: Thursday, November 06, 2003 1:24 PM To: 'HISTONET@PATHOLOGY.SWMED.EDU' Subject: [Histonet] LOOKING FOR ANTIBODY - alpha-methylacyl-CoA Racemase (AMACR) Hello to Everyone, I need help please where to order alpha methyl-CoA Racemase (AMACR). Thank you so much. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Thu Nov 6 13:50:30 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] DNA extraction from wax sections In-Reply-To: Message-ID: I have a protocol for melting tissue and cutting it up and then extracting DNA from the unembedded tissue. Is this what you want. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Michele Ellender Sent: Thursday, November 06, 2003 8:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DNA extraction from wax sections Dear all, Does anyone out there have a protocol to extract DNA from sections of formalin fixed wax embedded mouse tissues? I've been asked to microdissect GI tumours from wax sections and extract the DNA for use in PCR techniques. Hope someone can help. Many thanks, Michele Dr Michele Ellender Radiation Effects Department, NRPB, Chilton, Didcot, Oxon. OX11 0RQ. UK michele.ellender@nrpb.org This e-mail transmission is strictly confidential and intended solely for the person or organisation to whom it is addressed. It may contain privileged and confidential information and if you are not the intended recipient, please do not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, please notify the sender as soon as possible and delete the message. Please note that NRPB monitors incoming and outgoing e-mail for compliance with its Acceptable Use Policy. This will include scanning incoming e-mails to detect viruses and key-words and may in some circumstances result in the manual monitoring of the content of messages. National Radiological Protection Board E-mail: nrpb@nrpb.org Web site: www.nrpb.org -------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Thu Nov 6 14:06:31 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] CD4/CD8 In-Reply-To: <6.0.0.22.0.20031106125401.02998ca0@127.0.0.1> Message-ID: The problem I have found trying to do T subsets on mouse ffpe tissue is that the antibodies are mouse monoclonals and so you must use a mouse on mouse biotinylation detection system ( or at least I have not found a non-biotin mouse on mouse detection, if there is one out there please let me know) so with this biotin system you can get a lot of endogenous biotin background even with serious AB block methods. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Amos Brooks Sent: Thursday, November 06, 2003 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD4/CD8 Manuelle, I would certainly recommend using Envision (or some non-biotin based detection system). Both antibodies work best using high pH retrieval which tends to necessitate Envision. I can't speak for Dako CD4 as we use Novocastra but we get much better results doing the peroxidase block prior to antigen retrieval. The H2O2 interferes with the signal. It sounds like a pain but the results are clearly better. good luck, Amos Brooks At 11:35 AM 11/6/03, you wrote: >Message: 19 >Reply-To: "Manuelle De Bock" >From: "Manuelle De Bock" >To: >Date: Thu, 6 Nov 2003 11:36:03 +0100 >Subject: [Histonet] CD 4/CD 8 staining of frozen stomach sections of mice > >This is a multi-part message in MIME format. > >------=_NextPart_000_000F_01C3A45A.2907E120 >Content-Type: text/plain; > charset="iso-8859-1" >Content-Transfer-Encoding: quoted-printable > >Hello everyone, > >I am trying (for a long time allready) to produce a good CD4/CD8 = >staining of frozen stomach tissue (mice). We use the Dako rat anti-mouse = >CD4 antibody but always encounter a large amound of background after = >staining (it seems like there is a lot of cross reactivity with the = >glandular epithelium). Can anyone help me to solve this problem ? >Thanx ! > > >Manuelle De Bock, dierenarts (DVM) >Laboratory of Veterinary Pathology=20 >Department of Pathology, Bacteriology and Avian Diseases >Faculty of Veterinary Medicine >Ghent University >Salisburylaan 133 >B9820 Merelbeke - Belgium >Tel 0032(0)9 264 7745 >Fax 0032(0)9 264 7789 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carolynluty <@t> hotmail.com Thu Nov 6 14:18:21 2003 From: carolynluty <@t> hotmail.com (Carolyn Luty) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] giemsa Message-ID: I am looking for a quick method of a giemsa stain for a tsank smear. _________________________________________________________________ Help STOP SPAM with the new MSN 8 and get 2 months FREE* http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From yylo <@t> ucdavis.edu Thu Nov 6 14:31:09 2003 From: yylo <@t> ucdavis.edu (Yeung Lo) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] total mercury staining in mouse brain Message-ID: <200311062031.hA6KV9a09444@adalia.ucdavis.edu> Hi All, We are able to confirm mercury accumulation in mouse brains which we have injected mercury solution into subcutaneously at the scruff region. And now we would like to stain for the mercury to see where it's located. Is there any good staining method for organic+inorganic mercury out there? What would be the best way to prep the brain? Will perfursion with paraformaldehyde be fixing the brain too much and "drain" out some of the organic mercury? Look forwards to hearing from you. Yeung Y. Lo Dept. Neurological Surgery School of Medicine University of California, Davis yylo@ucdavis.edu yeunglo@hotmail.com 530-752-0753 (office) 530-304-7368 (cell) From tissuearray <@t> hotmail.com Thu Nov 6 14:38:25 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Re: tape Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/6d253032/attachment.htm From psych348 <@t> hotmail.com Thu Nov 6 14:48:20 2003 From: psych348 <@t> hotmail.com (Psych 348) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Rainbow Trout Brains slicing and BrDU staining Message-ID: Hi, Has anyone done slicing with rainbow trout brains and staining with BrDU afterwards?. The BrDU staining protocol I am using right now was initially designed for rat and mice brains tissues and it seems to be too vigorous for the fish brain slices (too small). I originally sliced out 30 microns section and left them on slides sitting on ice; then I put the slides into a 60 degree water bath for 2 hours to melt down the wax (not entirely melted) and denature the DNA. After overnight drying they seemed fine but some if not all of the tissues come off during the BrDU staining. I still have to put them into the cresyl violet stain so I don't think I'll have anything left afterwards! I'd appreciate any kind of feedback, thanks a lot! Cheers, Siu Him Chan University of British Columbia _________________________________________________________________ The new MSN 8: smart spam protection and 2 months FREE* http://join.msn.com/?page=features/junkmail http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From CCLYATT <@t> mail.mcg.edu Thu Nov 6 15:42:06 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Position Opening Message-ID: I have two positions open for HT(ASCP) histology techs at the Medical College of Georgia in Augusta, Georgia. Great climate and easy drive to beaches, mountains and major cities. Golf, horses, tennis, lake resorts, baseball, hockey, swimming, theater, crafts and more. Augusta and surrounding areas has it all for adults and children as well. Contact Jennifer Boynton in Human Resources (706-721-3921). Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 e-mail: cclyatt@mail.mcg.edu From CTague <@t> ahs.llumc.edu Thu Nov 6 15:53:31 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Position Opening Message-ID: can you get me on at Augusta National as part of an incentive package? LOL -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Claye Clyatt Sent: Thursday, November 06, 2003 13:42 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Position Opening I have two positions open for HT(ASCP) histology techs at the Medical College of Georgia in Augusta, Georgia. Great climate and easy drive to beaches, mountains and major cities. Golf, horses, tennis, lake resorts, baseball, hockey, swimming, theater, crafts and more. Augusta and surrounding areas has it all for adults and children as well. Contact Jennifer Boynton in Human Resources (706-721-3921). Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 e-mail: cclyatt@mail.mcg.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From ploykasek <@t> phenopath.com Thu Nov 6 16:15:06 2003 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] DNA extraction from wax sections In-Reply-To: Message-ID: There is a product called Gene Releaser from Bioventures, Inc that works well for extracting DNA from cut sections. Patti > I have a protocol for melting tissue and cutting it up and then extracting > DNA from the unembedded tissue. Is this what you want. > Patsy > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Michele > Ellender > Sent: Thursday, November 06, 2003 8:55 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] DNA extraction from wax sections > > Dear all, > Does anyone out there have a protocol to extract DNA from sections of > formalin fixed wax embedded mouse tissues? > I've been asked to microdissect GI tumours from wax sections and extract the > DNA for use in PCR techniques. > Hope someone can help. > Many thanks, > Michele > > Dr Michele Ellender > Radiation Effects Department, > NRPB, Chilton, Didcot, > Oxon. OX11 0RQ. UK > > michele.ellender@nrpb.org > > > > This e-mail transmission is strictly confidential and intended > solely for the person or organisation to whom it is > addressed. It may contain privileged and confidential > information and if you are not the intended recipient, > please do not copy, distribute or take any action in > reliance on it. If you have received this e-mail in error, > please notify the sender as soon as possible and delete > the message. > > Please note that NRPB monitors incoming and outgoing > e-mail for compliance with its Acceptable Use Policy. This > will include scanning incoming e-mails to detect viruses > and key-words and may in some circumstances result in > the manual monitoring of the content of messages. > > National Radiological Protection Board > E-mail: nrpb@nrpb.org > Web site: www.nrpb.org > -------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegg <@t> colobio.com Thu Nov 6 16:30:33 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Re: tape In-Reply-To: Message-ID: I can witness to the value of using the tape transfer method for frozen whole calcified rat femurs, try doing that without the tape system. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Thom Jensen Sent: Thursday, November 06, 2003 1:38 PM To: murphy.linda@mayo.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: tape Linda, Thank you for your input. Not a lot of people want to talk about the problems with the Tape-Transfer System. We also have two in our lab and I have tried on several occasions to work with Instrumedics to understand how it can be used. Eventually they stopped emailing me which ended our collaboration. Now I don't recommend the tape method to anyone. I have published two articles on Array Instruction in The Journal of Histotechnology and I thought at one time that the Tape Method could me a good alternative for techs that have a problem cutting arrays. But now I tell them to find someone in their area that can cut well. Thanks again, Thom Jensen For more information on Tissue Microarray Instruction visit: www.arrayworkshop.com >From: "Murphy, Linda M." >To: "'tissuearray@hotmail.com'" >Subject: tape >Date: Thu, 6 Nov 2003 13:29:53 -0600 > >Tom, >I am so excited that people are publicly voicing just how they feel about this most horrendous tape method. First off, I have felt all along that it is an insult to any HistoTech who cuts........the two technicians who do the cutting in my lab cut absolutely gorgeous sections.......special attention is paid to the TMA's and they get consistent beautiful sections with little to no distortions. My lab director was so impressed, however, by the articles she had read about the "success" of the tape method, we were asked to buy one for each cutting station.....they now just sit in the drawer in case a researcher feels the need. I have a feeling we will end up tossing them in due time. I am all for the poll. I no longer participate in the Histonet and was given this information from a friend. You are certainly more than welcome to express my views if you like. I would though like to state this is nothing against Bernice and Instrumedics. They have known for quite some time that their tape method needs work. I would hope they would benefit from the information they would receive from a poll such as you would like to undertake. Please feel free to contact me at any time.......thanks. Linda > >Linda M. Murphy, HT(ASCP) >Supervisor, TACMA Shared Resource >10-36 Guggenheim >Mayo Foundation >Rochester, MN 55905 >Voice:(507)266-5115 >Fax:(507)284-8105 >email:murphy.linda@mayo.edu > _____ Concerned that messages may bounce because your Hotmail account is over limit? Get Hotmail Extra Storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/765d8516/attachment.htm From la.sebree <@t> hosp.wisc.edu Thu Nov 6 16:24:18 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Re: tape Message-ID: In all fairness I feel that I have to add my $.02 worth. In my last research position (a number of years ago) I needed to section cross sections of frozen rabbit heart that had had radioactive elements perfused through it. We needed adjacent sections that would be as identical as possible in order to overlay autoradiographic images and histochemically stained images. Using the Instrumedics tape transfer system, I was able to produce exquisite, near identical sections that enabled us to compile the data we needed for our research project. Granted, I never tried the system with paraffin sectioning but at least in sectioning frozens, the system worked brilliantly for me. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Thom Jensen [mailto:tissuearray@hotmail.com] Sent: Thursday, November 06, 2003 2:38 PM To: murphy.linda@mayo.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: tape Linda, Thank you for your input. Not a lot of people want to talk about the problems with the Tape-Transfer System. We also have two in our lab and I have tried on several occasions to work with Instrumedics to understand how it can be used. Eventually they stopped emailing me which ended our collaboration. Now I don't recommend the tape method to anyone. I have published two articles on Array Instruction in The Journal of Histotechnology and I thought at one time that the Tape Method could me a good alternative for techs that have a problem cutting arrays. But now I tell them to find someone in their area that can cut well. Thanks again, Thom Jensen For more information on Tissue Microarray Instruction visit: www.arrayworkshop.com >From: "Murphy, Linda M." >To: "'tissuearray@hotmail.com'" >Subject: tape >Date: Thu, 6 Nov 2003 13:29:53 -0600 > >Tom, >I am so excited that people are publicly voicing just how they feel about this most horrendous tape method. First off, I have felt all along that it is an insult to any HistoTech who cuts........the two technicians who do the cutting in my lab cut absolutely gorgeous sections.......special attention is paid to the TMA's and they get consistent beautiful sections with little to no distortions. My lab director was so impressed, however, by the articles she had read about the "success" of the tape method, we were asked to buy one for each cutting station.....they now just sit in the drawer in case a researcher feels the need. I have a feeling we will end up tossing them in due time. I am all for the poll. I no longer participate in the Histonet and was given this information from a friend. You are certainly more than welcome to express my views if you like. I would though like to state this is nothing against Bernice and Instrumedics. They have known for quite some time that their tape method needs work. I would hope they would benefit from the information they would receive from a poll such as you would like to undertake. Please feel free to contact me at any time.......thanks. Linda > >Linda M. Murphy, HT(ASCP) >Supervisor, TACMA Shared Resource >10-36 Guggenheim >Mayo Foundation >Rochester, MN 55905 >Voice:(507)266-5115 >Fax:(507)284-8105 >email:murphy.linda@mayo.edu > _____ Concerned that messages may bounce because your Hotmail account is over limit? Get Hotmail Extra Storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/97bad96b/attachment.htm From GREYTRUNK <@t> aol.com Thu Nov 6 17:37:08 2003 From: GREYTRUNK <@t> aol.com (GREYTRUNK@aol.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Wilms' Tumor WT1 Message-ID: <38.3e73750c.2cdc3524@aol.com> We use papillary serous ovarian cancer tissue--much easier to come by. Roxanne -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031106/f52df9c7/attachment.htm From nheuer <@t> anhb.uwa.edu.au Thu Nov 6 19:07:12 2003 From: nheuer <@t> anhb.uwa.edu.au (Natascha Heuer) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Interested in buying automated staining device in Australia Message-ID: Hello, i was interested in hearing from anyone in Australia (preferably Perth but i'll take what i can get!!) who would like to sell an automated stainer ( new or pre-loved) programmable for haematoxylin and eosin routine's, or where i can purchase such machines. If you could include the model, year and a price, i would be really appreciative. Cheers Natascha Heuer The University of Western Australia School of Anatomy & Human Biology 35 Stirling Hwy Crawley WA 6009 Ph: (08) 9380 3798 Fax: (08) 9380 1051 Email: nheuer@anhb.uwa.edu.au From hadi83 <@t> comcast.net Fri Nov 7 02:50:25 2003 From: hadi83 <@t> comcast.net (Hadi Yaziji) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Genetics anyone? In-Reply-To: <8CD0CDC791DCC343ABB1F15298E4F3C417CCB9@usca0082k03.rallansci.apogent.com> References: <8CD0CDC791DCC343ABB1F15298E4F3C417CCB9@usca0082k03.rallansci.apogent.com> Message-ID: <6E18F05D-10FF-11D8-8671-00039378E76A@comcast.net> More on ploidy: Using "stain" is most likely referring to Feulgen stain. With image analysis it becomes more accurate, but still semiquantitative. Success depends on the image analysis software and experience of operator. Flow cytometry requires grinding either fresh tissue or 50 microns of the tissue block. It's certainly more quantitative. FISH is the enumeration of chromosome copies. On tissue sections (using 2-6 micron sections), chromosome centromere probes of more than one chromosome (preferably 3) are used in the same kit. This is as close to quantitation as it gets. It is accurate, and relatively easy to use. Cytogenetic spread is the real quantitative test, but it requires fresh tissue sample and growing it in cell culture, just like amniocentesis testing for fetal chromosomal abnormality. Software image analysis assistance is also available for the last two. As colleagues already indicated, your researcher is most likely implying "Fuelgen" stain. Best, Hadi Yaziji, M.D. PhenoPath Laboratories On Nov 6, 2003, at 8:20 AM, Morken, Tim - Labvision wrote: > Cheryl, As noted by others, ploidy can be determined using the feulgen > stain. While a simple bright-field examination can show differences in > stain > intensity related to ploidy that would be a very rough guess as to > actual > ploidy differences. More exact analysis requires either an image > analysis > system (several on the market) or flow cytometry. > > Here is a concise explanation of one method of ploidy analysis: > http://www.fairimag.co.uk/fairfield/dna-cytometry/ploidy-feulgen.html > > > Tim Morken > Lab Vision / NeoMarkers > www.labvision.com > > > -----Original Message----- > From: Cheryl Crowder [mailto:ccrowder@mail.vetmed.lsu.edu] > Sent: Thursday, November 06, 2003 5:47 AM > To: Histonet > Subject: [Histonet] Genetics anyone? > > Good morning - This questions was put to me yesterday and, knowing > little about genetics, I'm stumped. Can any of you help me. This > researcher is collecting samples which he says are either diploid, > triploid or tetraploid. He states that someone told him there is a > stain techniques that "would distinguish the 'ploidy' by the intensity > of the stained tissue". > > Have any of you heard of such a thing or know someone I can contact for > this "unusual" question? Thank you, in advance. Cheryl > > > Cheryl Crowder, BA, HTL(ASCP) > Chief Technologist > Anatomic Pathology > Department of Pathobiological Sciences > School of Veterinary Medicine > Louisiana State University > Skip Bertman Drive > Baton Rouge, LA ?70803 > > 225-578-9734 > FAX: ?225-578-9720 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Fri Nov 7 06:19:57 2003 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Reality check: Part 2 Message-ID: <002c01c3a529$75ab2b20$70494542@satx.rr.com> Hi, it's me again. What do you think about a company that promotes a better product that you test out, then decide to purchase that product, only to find out that the product doesn't work? I was given these ink pens that were supposed to be better than the Securline Markers. These pens were supposed to be alcohol and xylene resistant. The sample product worked great, so I purchased 100 of these pens. They worked for a few days, then bam, 25 cassettes lost all their numbers. Of course all these were GI cases. We were barely able to see what number was written on the cassettes. Gratefully, after many hours of matching the blocks to the gross descriptions, we were able to make new cassettes and match them up to the correct cases. So, as only I can do, I packaged up the cassettes that had the numbers washed off, put all these pens in a box with a letter explaining what happened and mailed them off to the company. That was a month ago. Now I know NSH was coming up so I let things go. Now, after one month and after the NSH, I still haven't heard back from this company. Is it me or are companies putting out inferior products these days? I know I sound like a whiner, but come on. What happened to taking responsibilities for your actions. I'm really not that difficult to get along with. I just want decent, dependable products. Am I holding these vendors at too much of a high standard? What ever happened to credibility? Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas From Barry.R.Rittman <@t> uth.tmc.edu Fri Nov 7 05:32:49 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Reality check: Part 2 Message-ID: <566FB0B522443D43AF02D2ADBE35A6F0635896@UTHEVS3.mail.uthouston.edu> Joe I think that you did the right thing. I would suggest that if you get no response within a week that you let us all know the name of the company so that we all (think that is good Texican) don't fall into the same trap. After all you and your hospital are at risk from potential lawsuits if GI cases get mixed and and as a result there is an erroneous diagnosis and treatment. It is unfortunate when this occurs. In fairness to the company it might simply be that there was a problem with manufcturing or the companies that suppied their inks etc.; however the lack of a response gives the perception that the company either realized that there was a problem and hoped that there would be very few complaints or they don't give a .......no excuse for lack of response. This attitude seems to be a trend with companies in general but most of the companies in our field that I have dealt with have been timely, very responsive and responsible in dealing with such problems....now the Bonneville that I used to have with its electrical problems was an entirely different matter. Give the company one more chance to respond and then send us the name. Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Fri 11/7/2003 6:19 AM To: Histonet Cc: Subject: [Histonet] Reality check: Part 2 Hi, it's me again. What do you think about a company that promotes a better product that you test out, then decide to purchase that product, only to find out that the product doesn't work? I was given these ink pens that were supposed to be better than the Securline Markers. These pens were supposed to be alcohol and xylene resistant. The sample product worked great, so I purchased 100 of these pens. They worked for a few days, then bam, 25 cassettes lost all their numbers. Of course all these were GI cases. We were barely able to see what number was written on the cassettes. Gratefully, after many hours of matching the blocks to the gross descriptions, we were able to make new cassettes and match them up to the correct cases. So, as only I can do, I packaged up the cassettes that had the numbers washed off, put all these pens in a box with a letter explaining what happened and mailed them off to the company. That was a month ago. Now I know NSH was coming up so I let things go. Now, after one month and after the NSH, I still haven't heard back from this company. Is it me or are companies putting out inferior products these days? I know I sound like a whiner, but come on. What happened to taking responsibilities for your actions. I'm really not that difficult to get along with. I just want decent, dependable products. Am I holding these vendors at too much of a high standard? What ever happened to credibility? Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ctsmolde <@t> capeheart.uct.ac.za Fri Nov 7 06:12:16 2003 From: ctsmolde <@t> capeheart.uct.ac.za (Jenny Molde) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] What am I going to do? Message-ID: Hi everyone out there Yes it`s me again. I have not been able to keep the stented sections on my slides. I have tried all the suggestions made by you wonderful people out there. It`s time to take early retirement as I have no other options left now. Thanks all for your input but none of it worked. Do any of you know if methylmethacrylate goes off for any reason? This is my last resort. Many thanks in advance. Jenny Molde Cardiovascular Research Unit University of Cape Town South Africa From tissuearray <@t> hotmail.com Fri Nov 7 07:24:20 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Paraffin Tape-Transfer System - POLL Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/18253ffd/attachment.htm From i.lombaert <@t> med.rug.nl Fri Nov 7 07:58:33 2003 From: i.lombaert <@t> med.rug.nl (Lombaert) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] cytokeratin Message-ID: <200311071358.OAA24204@medmail.med.rug.nl> Dear Histonetters, Has anyone tried to stain cytokeratins (7, 8.12, 14 and 18 from Sigma) on mouse/murine salivary gland tissues? I already tried 10 mM sodium citrate pH 6.0 for 30 min in the microwave, as well as 0.1M TrisHCl pH 9.5, no target retrieval at all, proteinase K for 5 min (20 mg/ml) but no results. I only could detect something with the enzym proteinase K, although my tissue was disrupted badly. My sections are paraffine imbedded and 4% formaldehyde fixed. Kind regards, Isabelle ----------------------- Ir. Isabelle Lombaert, MSc PhD student University of Groningen Faculty of Medical Sciences Antonius Deusinglaan 1 Building 3215, 5th floor, Room 553 9713 AV Groningen The Netherlands e-mail: I.Lombaert@med.rug.nl Tel.: +31 (0)50 363 29 15 From la.sebree <@t> hosp.wisc.edu Fri Nov 7 08:07:16 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Reality check: Part 2 Message-ID: Considering the potential catastrophic results that you were fortunately able to avoid, I think this vendor is shirking its responsibility. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, November 07, 2003 6:20 AM To: Histonet Subject: [Histonet] Reality check: Part 2 Hi, it's me again. What do you think about a company that promotes a better product that you test out, then decide to purchase that product, only to find out that the product doesn't work? I was given these ink pens that were supposed to be better than the Securline Markers. These pens were supposed to be alcohol and xylene resistant. The sample product worked great, so I purchased 100 of these pens. They worked for a few days, then bam, 25 cassettes lost all their numbers. Of course all these were GI cases. We were barely able to see what number was written on the cassettes. Gratefully, after many hours of matching the blocks to the gross descriptions, we were able to make new cassettes and match them up to the correct cases. So, as only I can do, I packaged up the cassettes that had the numbers washed off, put all these pens in a box with a letter explaining what happened and mailed them off to the company. That was a month ago. Now I know NSH was coming up so I let things go. Now, after one month and after the NSH, I still haven't heard back from this company. Is it me or are companies putting out inferior products these days? I know I sound like a whiner, but come on. What happened to taking responsibilities for your actions. I'm really not that difficult to get along with. I just want decent, dependable products. Am I holding these vendors at too much of a high standard? What ever happened to credibility? Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Nov 7 08:12:37 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Reality check: Part 2 Message-ID: I agree. I guess the other thing that disturbs me is the lack of response from the company. It is inexcusable. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Friday, November 07, 2003 9:07 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] Reality check: Part 2 Considering the potential catastrophic results that you were fortunately able to avoid, I think this vendor is shirking its responsibility. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, November 07, 2003 6:20 AM To: Histonet Subject: [Histonet] Reality check: Part 2 Hi, it's me again. What do you think about a company that promotes a better product that you test out, then decide to purchase that product, only to find out that the product doesn't work? I was given these ink pens that were supposed to be better than the Securline Markers. These pens were supposed to be alcohol and xylene resistant. The sample product worked great, so I purchased 100 of these pens. They worked for a few days, then bam, 25 cassettes lost all their numbers. Of course all these were GI cases. We were barely able to see what number was written on the cassettes. Gratefully, after many hours of matching the blocks to the gross descriptions, we were able to make new cassettes and match them up to the correct cases. So, as only I can do, I packaged up the cassettes that had the numbers washed off, put all these pens in a box with a letter explaining what happened and mailed them off to the company. That was a month ago. Now I know NSH was coming up so I let things go. Now, after one month and after the NSH, I still haven't heard back from this company. Is it me or are companies putting out inferior products these days? I know I sound like a whiner, but come on. What happened to taking responsibilities for your actions. I'm really not that difficult to get along with. I just want decent, dependable products. Am I holding these vendors at too much of a high standard? What ever happened to credibility? Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linke_Noelle <@t> Allergan.com Fri Nov 7 08:14:09 2003 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] EBV tissue Message-ID: <805C4A052A253B4A8E4948B13F54D12704D415@IRMAIL102.irvine.allergan.com> Hi all! Does anyone have a source for EBV+ FFPE tissue? Thank you! Noelle Linke, BS, HTL(ASCP) Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/fa068b44/attachment.htm From RITA.ANGEL <@t> UC.EDU Fri Nov 7 08:16:40 2003 From: RITA.ANGEL <@t> UC.EDU (Rita Angel) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] collagen I Message-ID: <5.1.0.14.2.20031107091529.00b9c978@ucmail3.uc.edu> Thanks to everyone that has responded to my question about the Collagen I on rabbit tissue. We will certainly be trying some of these out!! Rita Angel, HT University of Cincinnati From Jacquie.Mack <@t> CLS.ab.ca Fri Nov 7 08:22:38 2003 From: Jacquie.Mack <@t> CLS.ab.ca (Jacquie.Mack@CLS.ab.ca) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] benchtop cryostats Message-ID: <30C050525B881C4AAFF41E6D16543E6803487EB0@mail3.cls.ab.ca> Does anyone have any experience with benchtop cryostats? What are the advantages/disadvantages? Vendors welcome...need for availability in Canada. Thanks in advance Jacquie Jacqueline Mack Tech III , Anatomic Pathology Foothills Medical Center Calgary Laboratory Services ( (403)-944-4162 fax: (403)-270-4093 pager: 212-8223 # 0540 * Ja cquie.Mack@CLS.ab.ca Laboratory Medicine Enhancing Your Health Care -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/12eee56f/attachment.htm From dennijc <@t> vetmed.auburn.edu Fri Nov 7 08:25:39 2003 From: dennijc <@t> vetmed.auburn.edu (John C. Dennis) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] FITC-IgG In-Reply-To: <61135F0455D33347B5AAE209B903A304032622F9@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: If you haven't already, check with Molecular Probes. The Alexa 488 is swell. John Carroll Dennis Anatomy, Physiology, and Pharmacology 109 Greene Hall Auburn University, AL 36849 On Thu, 6 Nov 2003, Martha Ward wrote: > I am in search of a new vendor for our FITC-IgG that we use for the > direct immuno staining for renal and skin biopsies. Our current vendor, > BioWhittaker, informed me that their antibody is on back order until > mid-January. Any help I can get would be greatly appreciated. Thanks! > > Martha Ward > Wake Forest University Baptist Medical Center > From ian.montgomery <@t> bio.gla.ac.uk Fri Nov 7 08:25:59 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:10 2005 Subject: Fwd: [Histonet] cytokeratin Message-ID: <6.0.0.22.2.20031107142358.02d21ec0@udcf.gla.ac.uk> Isabelle, Used Cytokeratin 8 from Dako after Trysin on mouse tissue followed by Dako Ark. Beautiful results. Ian. >From: "Lombaert" >Organization: faculty of medical sciences (RuG) >To: histonet@lists.utsouthwestern.edu >Priority: normal >X-mailer: Pegasus Mail for Win32 (v3.12) >X-Scan-Signature: 976d3788468f6168a965852c1e2d4d6f >Sender: histonet-admin@lists.utsouthwestern.edu >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.0.13 >List-Help: >List-Post: >List-Subscribe: , > >List-Id: For the exchange of information pertaining to histotechnology and >related fields >List-Unsubscribe: , > > >List-Archive: >Date: Fri, 7 Nov 2003 14:58:33 +0100 >X-Scan-Signature: 690e9200c080ca55fd7a8d0fb0193eeb >X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu >Subject: [Histonet] cytokeratin >X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on > swlx167.swmed.edu >X-Spam-Status: No, hits=0.8 required=6.5 tests=MSGID_FROM_MTA_HEADER > autolearn=no version=2.60 >X-Spam-Level: >X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) >X-SA-Exim-Scanned: Yes >X-GLA-Spam-Filter: yes >X-GLA-Spam-Score: 0.9 (/) >X-GLA-Spam-Report: 0.9 MSGID_FROM_MTA_HEADER Message-Id was added by a relay > >Dear Histonetters, > >Has anyone tried to stain cytokeratins (7, 8.12, 14 and 18 from >Sigma) on mouse/murine salivary gland tissues? I already tried 10 >mM sodium citrate pH 6.0 for 30 min in the microwave, > >as well as 0.1M TrisHCl pH 9.5, >no target retrieval at all, >proteinase K for 5 min (20 mg/ml) > >but no results. I only could detect something with the enzym >proteinase K, although my tissue was disrupted badly. >My sections are paraffine imbedded and 4% formaldehyde fixed. > > >Kind regards, >Isabelle > > >----------------------- >Ir. Isabelle Lombaert, MSc >PhD student >University of Groningen >Faculty of Medical Sciences >Antonius Deusinglaan 1 >Building 3215, 5th floor, Room 553 >9713 AV Groningen >The Netherlands >e-mail: I.Lombaert@med.rug.nl >Tel.: +31 (0)50 363 29 15 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/a7786c4a/attachment.htm From DDDeltour <@t> sig.med.navy.mil Fri Nov 7 08:37:53 2003 From: DDDeltour <@t> sig.med.navy.mil (Deltour, Douglas D.(HM2)) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Reality check: Part 2 Message-ID: Do you use a different type of cassette for your GI cases? Maybe it is a compatibility problem between the two. I would do a couple of test runs to find out. The company still should investigate and keep you informed of the progress. HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy LPO Histology Histology Technician DSN 624-4669 FAX 624-4680 COMM (From US) 011-39-095-56-4669 DISCLAIMER: Confidentiality Notice - This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. **** Informazione Confidenziale - Questa e-Mail, incluso eventuali allegati, contiene informazioni confidenziali intese unicamente alla persona/e a cui e' indirizzata. Se avete ricevuto per errore questo messaggio, cortesemente contattare immediatamente il mittente e cancellare la e-Mail. Grazie****. -----Original Message----- From: Bartlett, Jeanine [mailto:jqb7@cdc.gov] Sent: Friday, November 07, 2003 3:13 PM To: Sebree Linda A. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reality check: Part 2 I agree. I guess the other thing that disturbs me is the lack of response from the company. It is inexcusable. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Friday, November 07, 2003 9:07 AM To: Joe Nocito; Histonet Subject: RE: [Histonet] Reality check: Part 2 Considering the potential catastrophic results that you were fortunately able to avoid, I think this vendor is shirking its responsibility. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, November 07, 2003 6:20 AM To: Histonet Subject: [Histonet] Reality check: Part 2 Hi, it's me again. What do you think about a company that promotes a better product that you test out, then decide to purchase that product, only to find out that the product doesn't work? I was given these ink pens that were supposed to be better than the Securline Markers. These pens were supposed to be alcohol and xylene resistant. The sample product worked great, so I purchased 100 of these pens. They worked for a few days, then bam, 25 cassettes lost all their numbers. Of course all these were GI cases. We were barely able to see what number was written on the cassettes. Gratefully, after many hours of matching the blocks to the gross descriptions, we were able to make new cassettes and match them up to the correct cases. So, as only I can do, I packaged up the cassettes that had the numbers washed off, put all these pens in a box with a letter explaining what happened and mailed them off to the company. That was a month ago. Now I know NSH was coming up so I let things go. Now, after one month and after the NSH, I still haven't heard back from this company. Is it me or are companies putting out inferior products these days? I know I sound like a whiner, but come on. What happened to taking responsibilities for your actions. I'm really not that difficult to get along with. I just want decent, dependable products. Am I holding these vendors at too much of a high standard? What ever happened to credibility? Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kosmicdog <@t> hotmail.com Fri Nov 7 08:54:18 2003 From: kosmicdog <@t> hotmail.com (jason madore) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] sectioning tissue mircroarrays Message-ID: Salut everyone, I am hoping that someone can give me pointers for sectioning of tissue microarrays. I am using leica RM2125 and the first array i need to section is a 0.06mm 400core ovarian serous tumour array. I have a problems with the cores rolling up as the section is cut. About half of the roled cores flatten out in the water bath but still losing half the array in this way is not good. Is the knife or some step prior to sectioning. The block was incubated at 37ds for half an hour. Any pointers on dealing with these problems or any other problems I might encounter would be greatly appreciated. Thanks in advance. I am of course checking the histonet archives as well. Ciao, J. _________________________________________________________________ MSN 8 with e-mail virus protection service: 2 months FREE* http://join.msn.com/?page=features/virus&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From gcallis <@t> montana.edu Fri Nov 7 10:08:11 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Murine CD4 and CD8 revisited Message-ID: <3.0.6.32.20031107090811.00bc8038@gemini.msu.montana.edu> Dear All, You wrote: I am trying (for a long time all ready) to produce a good CD4/CD8 staining of frozen stomach tissue (mice). We use the Dako rat anti-mouse CD4 antibody but always encounter a large amound of background after staining (it seems like there is a lot of cross reactivity with the glandular epithelium). Can anyone help me to solve this problem ? Thanx ! **************************************************************************** ********** You did not say how you do your actual staining, and if you use some of DAkO's kits with mutiple links secondaries, beware, they may be cross reacting with the mouse tissue if it antimouse included. We have a purist attitude in our lab, and do hundreds of murine IHC protocols a year, with a lot of CD4 and CD8 included, even double immunofluorescent staining with these two antibodies. Murine CD4 and CD8 ONLY WORKS ON FROZEN SECTIONS, NOT FFPE tissues, this has been reported on Histonet innumerable times as there is NO (yes, I am shouting!) retrieval of digestion method that will unmask those antigenic epitopes after formalin fixation. Sorry folks, just the mousie facts so don't waste your time. We never get background with our Murine CD4 or CD8. Our antibodies are monoclonals, rat antiMouse from Pharmingen, that is the first important step is to purchase the correct primary antibody. We work with either pure primary antibody or biotinylated primary and have equally good results. And with our biotinylated primary, coming back with SA-HRP, the dilution has gone out to 1:15,000 (0.5mg/ml). Frozen sections are air dried overnight at RT, then fixed with 75% acetone/25% absolute ethanol for 5 min at RT, then immediate rinses with DPBS. No substitution on alcohol, methanol is avoided at all costs. Preferred endog peroxidase block is glucose oxidase method. Normal serum block is 10% goat/2.5% mouse serum in Dulbeccos PBS, with 0.05% Tween 20 Use Strepavidin/biotin block from Vector rather than avidin/biotin block. Purified antibody is diluted in 1 - 5% goat, 30 min at RT You would have to do a dilution panel to determine optimal working concentration. Secondary antibody is Goat antiRat F(ab')2 frag of IgG adsorbed to mouse, so you done get cross reaction to fc receptors on tissue. 1:250 (0.5mg/ml) from Biosource/TAGO, 30 min RT SA-HRP 1:500 (0.5 mg/ml) from Biosource, 20 min AEC+ from DAKO - we control with microscope but usually around 2 - 5 min. We more often use Biotin conjugated rat antiMOuse CD4 or CD8 diluted in the NSB with 10% goat/2.5T mouse serum, same incubation time SA-HRP same as above AEC+ (primary is diluted 1:500 with this, could probably be less concentrated in your hands). If you use DAB+ from DAKO plus their enhancer, this is more sensitive and you can get that wonderful dilution out 1:15,000! Have pictures to prove it, and there is NO BACKGROUND. Use normal spleen as a control, the negative control is Isotype matched and with biotin-primary, the negative control must be biotinylated isotype matched IgG. We NEVER use whole IgG secondary antibodies with mouse, always F(ab')2 fragments, and Jackson's Donkey antiRat F(ab')2 fragment will work, but you must change your normal serum block to donkey rather than goat with 2.5% mouse serum. YOUR secondary MUST be adsorbed to mouse. We have also used mouse antiRat F(ab')2 fragment (Jackson) , but you must add Rat IgG to the diluent of the primary. Don't recall mg/ml on that, but could look it up, the results were still clean, no background here either. If you need a protocol, I will be happy to file attach privately. Also the glucose oxidase peroxidase blocking method. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From tissuearray <@t> hotmail.com Fri Nov 7 10:25:05 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] sectioning tissue mircroarrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/6c3df431/attachment.htm From Rcartun <@t> harthosp.org Fri Nov 7 10:26:56 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] DPC4 Message-ID: Is anyone doing IHC for "DPC4"? If so, where do you get your antibody? Thank you. Richard Cartun From john.mcginley <@t> colostate.edu Fri Nov 7 10:49:01 2003 From: john.mcginley <@t> colostate.edu (John McGinley) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Web Seminar - LCM and Microarray Analysis from Formalin Fixed Tissues Message-ID: <000601c3a54f$0c4fc660$29255281@MCGINLEY> Hi, For those who are interested in LCM and microgenomics I just got word that there will be a web seminar on the use formalin fixed tissue for DNA/RNA microarrays (see details below). Regards, John -------------------------- John N. McGinley, HTL(ASCP) Cancer Prevention Lab Colorado State University ---------------------------------------------------------------------------- --------------------- Web Seminar High Fidelity Microarray Analysis from Formalin Fixed and Frozen Tissues Using Microgenomics Speaker: Dr. Rajiv Raja, Director of Life Science, Arcturus Wednesday, November 19, 2003 Time: 9:00 - 10:00 A.M. Pacific Standard Time Microarrays have provided researchers with a powerful tool to analyze the transcriptional landscape of tissues. The ability to utilize precious tissue biopsies for global gene expression analysis using microarrays has been severely limited by the need to use frozen tissues for high quality RNA, and the need for several micrograms of total RNA. Arcturus has developed systems for performing microarray analysis from (a) nanograms of total RNA isolated from formalin-fixed tissue biopsies, both fresh and archived, and (b) picograms to nanograms of total RNA isolated from frozen tissue. These systems include methods for highly efficient extraction and isolation of RNA from microdissected samples or other small samples, and high yield amplification of isolated RNA. They also include protocols and procedures to analyze the quality of samples at various stages during the process. Data generated using these systems show high reproducibility and fidelity. Molecular signatures have been identified using as few as 500pg-1ng of RNA from frozen breast cancer biopsies and 5ng of RNA from formalin-fixed breast cancer biopsies. Further, differential gene expression between frozen and formalin-fixed samples demonstrates reasonable correlation and reliable data has been generated from archived tissue banks, suggesting direct applications in drug target discovery and the development of clinical diagnostic tests. Click the following link to enroll or to view other recorded events: http://arcturusevents.webex.com From RCHIOVETTI <@t> aol.com Fri Nov 7 10:47:23 2003 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] benchtop cryostats Message-ID: <25.40713f49.2cdd269b@aol.com> In a message dated 11/07/2003 7:22:26 AM US Mountain Standard Time, Jacquie.Mack@CLS.ab.ca writes: Does anyone have any experience with benchtop cryostats? What are the advantages/disadvantages? Vendors welcome...need for availability in Canada. Thanks in advance Jacquie Jacquie, Leica makes a really nice benchtop unit, the CM1100. Advantages: small, compact, good temperature stability, can run from DC voltages as well (e.g., battery supply), easily portable (it's a breeze to load it on a cart and move to other locations). Disadvantages: limited working/storage space inside, and temp control is best when the chamber door can be kept closed most of the time. So it's not a good choice for Mohs or other techniques where you need to keep lots of chucks and specimens frozen or where you need to work with the door open for long periods of time. Leica Customer Service from outside the USA: 847-405-0123 Cheers, Bob Chiovetti -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/8c11f636/attachment.htm From cfavara <@t> niaid.nih.gov Fri Nov 7 10:48:28 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Re: tape Message-ID: My $.02. I think there is a significant difference tape transfer for FROZEN sections and PARAFFIN. I have found the paraffin system to be more cumbersome and produce worse results than careful sectioning. However the system used for frozen sections can be extremely useful. Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu] Sent: Thursday, November 06, 2003 3:24 PM To: Thom Jensen; murphy.linda@mayo.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: tape In all fairness I feel that I have to add my $.02 worth. In my last research position (a number of years ago) I needed to section cross sections of frozen rabbit heart that had had radioactive elements perfused through it. We needed adjacent sections that would be as identical as possible in order to overlay autoradiographic images and histochemically stained images. Using the Instrumedics tape transfer system, I was able to produce exquisite, near identical sections that enabled us to compile the data we needed for our research project. Granted, I never tried the system with paraffin sectioning but at least in sectioning frozens, the system worked brilliantly for me. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Thom Jensen [mailto:tissuearray@hotmail.com] Sent: Thursday, November 06, 2003 2:38 PM To: murphy.linda@mayo.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: tape Linda, Thank you for your input. Not a lot of people want to talk about the problems with the Tape-Transfer System. We also have two in our lab and I have tried on several occasions to work with Instrumedics to understand how it can be used. Eventually they stopped emailing me which ended our collaboration. Now I don't recommend the tape method to anyone. I have published two articles on Array Instruction in The Journal of Histotechnology and I thought at one time that the Tape Method could me a good alternative for techs that have a problem cutting arrays. But now I tell them to find someone in their area that can cut well. Thanks again, Thom Jensen For more information on Tissue Microarray Instruction visit: www.arrayworkshop.com >From: "Murphy, Linda M." >To: "'tissuearray@hotmail.com'" >Subject: tape >Date: Thu, 6 Nov 2003 13:29:53 -0600 > >Tom, >I am so excited that people are publicly voicing just how they feel about this most horrendous tape method. First off, I have felt all along that it is an insult to any HistoTech who cuts........the two technicians who do the cutting in my lab cut absolutely gorgeous sections.......special attention is paid to the TMA's and they get consistent beautiful sections with little to no distortions. My lab director was so impressed, however, by the articles she had read about the "success" of the tape method, we were asked to buy one for each cutting station.....they now just sit in the drawer in case a researcher feels the need. I have a feeling we will end up tossing them in due time. I am all for the poll. I no longer participate in the Histonet and was given this information from a friend. You are certainly more than welcome to express my views if you like. I would though like to state this is nothing against Bernice and Instrumedics. They have known for quite some time that their tape method needs work. I would hope they would benefit from the information they would receive from a poll such as you would like to undertake. Please feel free to contact me at any time.......thanks. Linda > >Linda M. Murphy, HT(ASCP) >Supervisor, TACMA Shared Resource >10-36 Guggenheim >Mayo Foundation >Rochester, MN 55905 >Voice:(507)266-5115 >Fax:(507)284-8105 >email:murphy.linda@mayo.edu > _____ Concerned that messages may bounce because your Hotmail account is over limit? Get Hotmail Extra Storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/9ccee032/attachment.htm From Nancy.Walker <@t> sanofi-synthelabo.com Fri Nov 7 11:02:58 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] CB1 receptor Message-ID: Can anybody suggest a good antibody for the cannabinoid receptor type 1 for IHC on paraffin mouse tissue. There are so many out there that I heard don't work... thanks Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From dmikita <@t> wmcnet.org Fri Nov 7 11:31:58 2003 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Full-Time Histology Position open Message-ID: Hello Everyone, Wyoming Medical Center 1233 E. 2nd St. Casper, WY 82609 Job Code: 519 Posted: Oct-31-2003 - Position: Histologic Technician - Department: Anatomical Pathology - Salary: $11.50-17.20 - Full-time - Rotating shift One year certificate from college or technical school; and three to six months related experience and/or training; or equivalent combination of education and experience. ASCP registered, equivalent or eligible Contact: Mike Walker Tel: 800-526-5190 or 307-577-2406 Fax: 307-577-2579 From cathyeyre <@t> yahoo.com Fri Nov 7 11:39:20 2003 From: cathyeyre <@t> yahoo.com (cathy eyre) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] unsubcribe Message-ID: <20031107173920.81013.qmail@web40301.mail.yahoo.com> --------------------------------- Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/1437beea/attachment.htm From BMolinari <@t> heart.thi.tmc.edu Fri Nov 7 11:51:11 2003 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] RE: Looking for a lab to cut stents Message-ID: Tiffany's lab comes highly recommended by our lab. Betsy Molinari HT (ASCP) Texas Heart Institute -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Tiffany L Sheffield Sent: Wednesday, November 05, 2003 8:15 AM To: Bernard Ian R SSgt 59 CRES/MSROP Cc: 'Gordon Grant'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Looking for a lab to cut stents My lab does stents as well in Houston! We work with Texas Heart Institute in conjunction with other companies on a regular basis. We do thick saw cut ground and polished sections. Hope this helps! If you have any further questions feel free to contact me. Tiffany:) Bernard Ian R SSgt 59 CRES/MSROP wrote: Cathy MAYton in Arizona Does this. -----Original Message----- From: Gordon Grant [mailto:grantgd@comcast.net] Sent: Tuesday, November 04, 2003 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Looking for a lab to cut stents I was wondering if someone out there can give me a recommendation for a lab which can provide good quality sections of implanted stents. Specifically, we have been told the FDA likes the thick saw cut, ground and polished sections rather than microtomed sections. Thanks Gordon Grant -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, November 02, 2003 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet digest, Vol 1 #113 - 2 msgs Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-admin@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. appropriate pay for grossing techs (Jen Steinberg) 2. Re: appropriate pay for grossing techs (Gudrun Lang) --__--__-- Message: 1 Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST) From: Jen Steinberg To: histonet@lists.utsouthwestern.edu Subject: [Histonet] appropriate pay for grossing techs --0-1595893577-1067722359=:57919 Content-Type: text/plain; charset=us-ascii Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. --------------------------------- Do you Yahoo!? Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919 Content-Type: text/html; charset=us-ascii
Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers).  Would the pay be any different if the employee had an M.S.  The position is entry-level.  Thanks very much. 
 
 

Do you Yahoo!?
Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919-- --__--__-- Message: 2 From: "Gudrun Lang" To: "Histonetliste" , "Jen Steinberg" Date: Sun, 2 Nov 2003 13:19:09 +0100 Subject: Re: [Histonet] appropriate pay for grossing techs This is a multi-part message in MIME format. ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hi Jen, I dont have an answer for you, but another question. In Austria the = person, who does crossing, is a pathologist (m.d.).=20 We have several sorts of tissue f(rom toe to sculp) and I think without = a medical uni-study, it is very difficult. Even our doctors sometimes = make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message -----=20 From: Jen Steinberg=20 To: histonet@lists.utsouthwestern.edu=20 Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the = appropriate starting wage of a grossing tech would be for a private path = lab which works mainly on small skin, gyn, and oral specimens and = processes about 250 cases a day (between 2 grossers). Would the pay be = any different if the employee had an M.S. The position is entry-level. = Thanks very much. =20 ------------------------------------------------------------------------ -= ----- Do you Yahoo!? Exclusive Video Premiere - Britney Spears ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hi Jen,
I dont have an answer for you, but = another=20 question. In Austria the person, who does crossing, is a pathologist = (m.d.).=20
We have several sorts of tissue f(rom = toe to sculp)=20 and I think without a medical uni-study, it is very difficult. Even our = doctors=20 sometimes make mistakes and look over important details.
Is it usual in USA,  that = histotechs do=20 the crossing?
 
best wishes
Gudrun Lang
general hospital Linz, = Austria
----- Original Message -----
From:=20 Jen=20 Steinberg
To: histonet@lists.utsouth w= estern.edu=20
Sent: Saturday, November 01, = 2003 10:32=20 PM
Subject: [Histonet] appropriate = pay for=20 grossing techs

Hi,
   I'm hoping someone can help me.  I was = wondering what=20 the appropriate starting wage of a grossing tech would be for a = private path=20 lab which works mainly on small skin, gyn, and oral specimens and = processes=20 about 250 cases a day (between 2 grossers).  Would the pay be any = different if the employee had an M.S.  The position is = entry-level. =20 Thanks very much. 
 
 

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Exclusive Video Premiere - L= =3Dhttp://launch.yahoo.com/promos/britneyspears/">Britney=20 Spears ------=_NextPart_000_001B_01C3A143.E6310980-- --__--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/6955fe09/attachment.htm From Nancy.Walker <@t> sanofi-synthelabo.com Fri Nov 7 12:03:06 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] multi-specimen blocks Message-ID: Need advice from you tissue arrayers. I want to put on the same slides 10 specimens of about a 5mm diameter. When browsing the web about tissue microarray, this is what I understand from Thom Jensen's site http://www.arrayworkshop.com/index.html -punch out cores from existing blocks -place them in the holes of a recipient block that was punched when warm -place them upside down on a glass slide and warm to 37?C -press block on sides to set punches in paraffin -cool block before removing slide and cut with standard microtome techniques. Do you warm the existing blocks before punching? Any advice for a neophyte, other cool web sites etc. thanks, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From CTague <@t> ahs.llumc.edu Fri Nov 7 12:25:31 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] appropriate pay for grossing techs Message-ID: considering the amount of time the pathologist saves, i'd say something in the mid to high 20's would be appropriate. i think lumping a grossing tech in the same range as a routine H.T. is inaccurate... i do both which is why i support the seperation and a higher wage. in southern calif. the average H.T. is in the low to mid 20's. i'd love to hear any other input. curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jen Steinberg Sent: Saturday, November 01, 2003 13:33 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. _____ Do you Yahoo!? Exclusive Video Premiere - Britney Spears Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/a4000a89/attachment.htm From tracey.couse <@t> ibb.gatech.edu Fri Nov 7 14:22:40 2003 From: tracey.couse <@t> ibb.gatech.edu (Tracey Couse) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Success with Paraffin Tape Transfer System Message-ID: <5.0.0.25.2.20031107134347.00abf480@mail.ibb.gatech.edu> Hello to everyone, Personally, I feel it is unfair to make the general statement that the paraffin tape transfer system is not good for paraffin sections as was stated on Histonet. I have had success using the paraffin tape transfer system. I use it for both native tissue as well as bioengineered tissue samples. When I cannot get a section via any other means, it has provided sections for me to work with. This may not be the method of choice for routine histology, but can provide valuable information that otherwise may not be obtained from a problematic paraffin embedded sample. I feel that the degree of success with this system really depends on the application and histological goal of the user. (Actually, I have used it for both paraffin and frozen samples.) My 2 cents. Have a good weekend! Tracey Tracey Couse Georgia Tech/Emory Center for the Engineering of Living Tissues Georgia Institute of Technology From c.m.vanderloos <@t> amc.uva.nl Fri Nov 7 13:31:47 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] RE: Blue counterstain for immunos Message-ID: <4682a94689ba.4689ba4682a9@amc.uva.nl> Hi Cathy, How about the following simple trick: Use the Dako hematoxylin 1:10 diluted in distilled water! Apply to your FFPE section only for 15-20 seconds and rinse with tap water. Acetone- fixed cryostat sections even shorter (or higher diluted). Check the wet slide under the scope and judge if it needs perhaps another 15 seconds. Success! Chris van der Loos Academical Medical Center Dept. of Cardiovascular Pathology Amsterdam - The Netherlands >From Cathy.Crumpton@tuality.org Date Wed, 5 Nov 2003 12:48:42 -0800 To histonet@lists.utsouthwestern.edu Subject [Histonet] Blue counterstain for immunos Hi all. One of my pathologists saw an immuno stain that had a light blue counterstain and, of course, he would like us to try "something like that". Would anyone have any suggestions as to what kind of counterstain would be good for the light blue? We use DAKO products and other than hematoxylin and methyl green they did not have any suggestions. We already use hematoxylin. Thanks! From jlambrey <@t> hotmail.com Fri Nov 7 14:28:43 2003 From: jlambrey <@t> hotmail.com (Julien Lambrey de Souza) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Help on Teared tissues Message-ID: Hello all, Could someone give me the various reasons why tissues are torn on slides. In my case I doubt that this is due to the microtome. Is processing in parafin a possibility? Maybe longer times in 100% alchool, Xylen and then parafin? Thanks, Julien De Souza. _________________________________________________________________ The new MSN 8: advanced junk mail protection and 2 months FREE* http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From gcallis <@t> montana.edu Fri Nov 7 14:54:35 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Help on Teared tissues In-Reply-To: Message-ID: <3.0.6.32.20031107135435.00bc88d8@gemini.msu.montana.edu> Tearing of sections can happen for many reasons, but probably a dull knife or a knife that has knicks and gouges on its sharp edge. Change the knife often, disposable knife blades are far nicer for this reason, move to sharp non-defective edge and keep brushes or forceps, whatever you use to pick up sections off the blade - AWAY FROM THAT EDGE. Paraffin buildup on back of knife and holder is a possibility. Learn to clean a knife properly, use a solvent to remove paraffin, and wipe and away from the edge rather than go over it with any type of guaze or tissue. Xylene tends to harden the tissue a bit more. There has been a great deal of discussion on sectioning problems on Histonet, do a search of the Histonet Archives to see what has been discussed in the past. Are all your screws, knife holder settings tight? Microtomes can have problems and sneak up on you when you least expect it. Improper processing may be a factor, but it is more likely due to overexposure to solvents rather than increase time, or not enough paraffin infiltration. If you put out exactly how you process your tissue, the processing schedule and WHAT tissues you are working with, Histonetters could help you more. Some tissues are more susceptible to friable, torn sections and are you working with animal tissues? If so, what species? Give us a bit more information please. At 08:28 PM 11/7/2003 +0000, you wrote: >Hello all, > >Could someone give me the various reasons why tissues are torn on slides. In >my case I doubt that this is due to the microtome. Is processing in parafin >a possibility? Maybe longer times in 100% alchool, Xylen and then parafin? > >Thanks, >Julien De Souza. > >_________________________________________________________________ >The new MSN 8: advanced junk mail protection and 2 months FREE* >http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.ms n.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From histo20 <@t> hotmail.com Fri Nov 7 14:59:55 2003 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] 2 cytology questions Message-ID: Hello everyone, CLIA visited our institution a few months ago. Among their findings was the fact that our Cytology Procedure Manual did not indicate patient preparation for the collection of medical and gynecological specimens. Does anyone else include this information in their manuals? We are also thinking about automating the staining in the Cytology Department. Currently, we share a Leica stainer with the Histology Department, but we only stain PAPS in the afternoon. We are thinking about automating the medicals. Does anyone have any suggestions or experience with this? Thanks so very much for all of your help! Paula Wilder St. Joseph Medical Center Towson, MD 21204 _________________________________________________________________ Is your computer infected with a virus? Find out with a FREE computer virus scan from McAfee. Take the FreeScan now! http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From histo20 <@t> hotmail.com Fri Nov 7 15:03:09 2003 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Artisan Dakocytomation Validation Message-ID: We just recently purchased an Artisan Specials stainer. We do all of the specials and then some offered on the stainer. Our pathologists want to validate 5 surgical cases for each special stain that we do. They also then asked if I would post this message to see what other institutions have done for validation. Thanks so much for any and all help. IT is much appreciated! Paula Wilder St. Joseph Medical Center Towson, MD 21204 _________________________________________________________________ Concerned that messages may bounce because your Hotmail account is over limit? Get Hotmail Extra Storage! http://join.msn.com/?PAGE=features/es From prippstein <@t> ottawahospital.on.ca Fri Nov 7 15:45:17 2003 From: prippstein <@t> ottawahospital.on.ca (Rippstein, Peter) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] unsubscribe please Message-ID: <5D1996791093D511BBCA000103CF3D6B023BB6C4@C-CIV-EXCH02> Peter Rippstein, ART, MLT Charge Technologist Anatomical Pathology The Ottawa Hospital, Civic Campus Ph: 798-5555 ext 16589 Fax: 761-4846 email: prippstein@ottawahospital.on.ca From Reuel.Cornelia <@t> tsrh.org Fri Nov 7 15:53:33 2003 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] looking for nena dimaano Message-ID: hello nena, you promised me to send me some of your reviewers for ASCP (histo). Now that i have registerd myself for the exam, i am asking your help to provide me any information where to get those get used reviewers aside from the one provided by ascp online without too much burden form my pocket.he,he,he. hope for your response soon. If anyone happen to read this, you can help me too by providing your used reviewers to me and i'll pay for hte shipping. thanks.Opps...if it will be for free then it would be much appreciated. Thank you. Reuel Cornelia TSRH Cellular Pathology 2222 Welborn St. Dallas, TX 75219 214-559-7699 fax 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning differences. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031107/39eab3ca/attachment.htm From slieblich <@t> shaw.ca Fri Nov 7 16:18:52 2003 From: slieblich <@t> shaw.ca (slieblich@shaw.ca) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] immunofluorescence problem Message-ID: <4f32df4f34de.4f34de4f32df@shaw.ca> I am doing triple labeling immuno fluorescence of mouse brain tissue and am having some difficulties. My three markers are NeuN, GFAP, and BrdU. Previously when I have triple labeled, I only get gfap and brdu fluorescence and no NeuN (or rarely and inconsistently). I have found out that it is possible that the acid used to denature the DNA for BrdU may be interfering with NeuN antibodies. I was wondering if anyone has also had this problem with NeuN. Does anyone have any ideas to get around the NeuN/hydrochloric acid issue? Thanks! Stephanie Lieblich Cellular Neurophysiology Lab University of British Columbia Vancouver, B.C., Canada From ccrowder <@t> mail.vetmed.lsu.edu Fri Nov 7 16:25:34 2003 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Diploidy Message-ID: I would like to thank all of you who gave me answers to my diploidy, etc. question. I should have thought of the Feulen stain, but guess by now I'm rather brain dead. The researcher was very impressed with the quick response. Thank you all again. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 From lao_ji <@t> yahoo.com Fri Nov 7 16:38:43 2003 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Whole mount in situ for immature RNA In-Reply-To: <3FA2AF4A.FB23D1C1@uwo.ca> Message-ID: <20031107223843.96951.qmail@web12505.mail.yahoo.com> Any people has experience in whole mount in situ for immature RNA? I thought the immature RNA is staying in nuclus, while the spliced RNA in cytoplasma, which the Proteinase K can help penetrate. Who can recommend something help to penetrate nucleus membrane? Thanks! Jimmy __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From doscwk <@t> nus.edu.sg Fri Nov 7 20:32:32 2003 From: doscwk <@t> nus.edu.sg (Chan Wai Kam) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Microwave tissue processors Message-ID: Thanks so much to all of you who responded to my queries. Julee Chan Orthopaedic Surgery National University of Singapore From mucram11 <@t> earthlink.net Sat Nov 8 06:16:54 2003 From: mucram11 <@t> earthlink.net (Pamela Marcum) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Help on Teared tissues Message-ID: <410-220031168121654500@earthlink.net> I agree with everything Gayle has said and more information will help. You may also want to look at how you pick sections up off the water bath or how you transfer them. You method of pick up and water bath temperature would also help. Pam Marcum > [Original Message] > From: Gayle Callis > To: Julien Lambrey de Souza ; > Date: 11/7/2003 3:55:02 PM > Subject: Re: [Histonet] Help on Teared tissues > > Tearing of sections can happen for many reasons, but probably a dull knife > or a knife that has knicks and gouges on its sharp edge. Change the knife > often, disposable knife blades are far nicer for this reason, move to sharp > non-defective edge and keep brushes or forceps, whatever you use to pick up > sections off the blade - AWAY FROM THAT EDGE. Paraffin buildup on back of > knife and holder is a possibility. Learn to clean a knife properly, use a > solvent to remove paraffin, and wipe and away from the edge rather than go > over it with any type of guaze or tissue. Xylene tends to harden the tissue > a bit more. There has been a great deal of discussion on sectioning > problems on Histonet, do a search of the Histonet Archives to see what has > been discussed in the past. > > Are all your screws, knife holder settings tight? Microtomes can have > problems and sneak up on you when you least expect it. > > Improper processing may be a factor, but it is more likely due to > overexposure to solvents rather than increase time, or not enough paraffin > infiltration. > > If you put out exactly how you process your tissue, the processing schedule > and WHAT tissues you are working with, Histonetters could help you more. > Some tissues are more susceptible to friable, torn sections and are you > working with animal tissues? If so, what species? Give us a bit more > information please. > > > > > > At 08:28 PM 11/7/2003 +0000, you wrote: > >Hello all, > > > >Could someone give me the various reasons why tissues are torn on slides. In > >my case I doubt that this is due to the microtome. Is processing in parafin > >a possibility? Maybe longer times in 100% alchool, Xylen and then parafin? > > > >Thanks, > >Julien De Souza. > > > >_________________________________________________________________ > >The new MSN 8: advanced junk mail protection and 2 months FREE* > >http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.ms > n.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 (lab with voice mail) > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khor0011 <@t> flinders.edu.au Sat Nov 8 06:52:12 2003 From: khor0011 <@t> flinders.edu.au (khor0011@flinders.edu.au) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene Message-ID: <1068295932.3face6fc662f2@imap.flinders.edu.au> Hi everyone, I am an Honours student, currently finishing up my Honours thesis. I am wondering if anyone could give me some REFERENCES on snap freezing mouse tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY for snap freezing my mouse tissues in my project, but not with isopentene. And i have been having cryostat sectioned mouse tissues that have a lot of tissue degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i got the idea from someone that this maybe due to the fact that i did not use isopentene in the snap freezing process. So what i want is to discuss this in my thesis. So it would be great if someone could give me any scientific references/comments/advice on this method whereby isopentene is used in the snap freezing process, and why using this is important. I have been doing some internet searching myself, but any advice or help from all histo experts out there would be greatly appreciated. Best regards, Hong Yuan Khor Flinders University South Australia From pengbaowei <@t> 21cn.com Sat Nov 8 09:47:35 2003 From: pengbaowei <@t> 21cn.com (Baowei Peng) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] counterstain for GFP with DAPI Message-ID: Dear all, How to improve quality of counterstain for GFP with DAPI. I'm always lost GFP fluoresence especially in those cells expressed GFP weakly after counterstain with DAPI. Baowei Peng Shanghai JiaoTong University, PRC ---------------------------------------------- Ó”±§ÀËÂțŁŹłąÊÔŒ€Ç飏ŒÓÈ뿥ÄĐĂÀĆź”ÄŒ€Ç霻ÓŃÀÖÔ° http://y.21cn.com 21cnĐÂÎĆ żìžĐĐÂÎĆ http://news.21cn.com žöÈËÍűŐŸÁśÁż±äœđÇźŁŹ»¶Ó­ŒÓÈë21CNÓÊŒțÁȘĂËŁĄ http://mail.21cn.com/alliance/ ŸȘÆŰ:Ä«Îśžç6ž»ÎÌ10ÄêČĐɱ100ÉÙĆź http://news.21cn.com/social/ From peptolab <@t> hamptons.com Sat Nov 8 12:54:07 2003 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Dakocytomation Artisan Validation Message-ID: <000901c3a629$b1de9450$dca5bd18@JEFF> We simply run our known positive control for each stain or antibody protocol and examine for optimal staining.I think you will find just that- the stains are very nice. We are careful to use in house controls fixed and processed just as our patient's specimens- in fact our controls ARE our patient's specimens. We do the same for each new lot number. For ER and PR we did compare ten blocks that had previously been sent to our reference lab for staining against our in house stains before going online with ER/PR. I also run many antibodies not in the Artisan menu doing the antigen retrieval and antibody incubation off the machine and using Artisan ABC Detection. Artisan is a lovely platform to use. Jeff Silverman HT HTL QIHC Pathologists" Assistant Southside Hospital Bay Shore NY USA From jkiernan <@t> uwo.ca Sun Nov 9 00:43:10 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene References: <1068295932.3face6fc662f2@imap.flinders.edu.au> Message-ID: <3FADE1FE.290282BA@uwo.ca> There is abundant advice for you in textbooks written 40 years ago (and more recently too). ASK YOUR SUPERVISOR to suggest books or review articles. For an honours-year project you should be guided by your local boss and the literature, not by answers from an Internet listserver. Anyone can answer question. I could have answered yours with all sorts of fake rubbish. Would you have ueed it in your honours dissertation? -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ____________________________________________ khor0011@flinders.edu.au wrote: > > Hi everyone, > > I am an Honours student, currently finishing up my Honours thesis. > I am wondering if anyone could give me some REFERENCES on snap freezing mouse > tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY > for snap freezing my mouse tissues in my project, but not with isopentene. And > i have been having cryostat sectioned mouse tissues that have a lot of tissue > degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i > got the idea from someone that this maybe due to the fact that i did not use > isopentene in the snap freezing process. > > So what i want is to discuss this in my thesis. So it would be great if > someone could give me any scientific references/comments/advice on this method > whereby isopentene is used in the snap freezing process, and why using this is > important. > > I have been doing some internet searching myself, but any advice or help from > all histo experts out there would be greatly appreciated. > > Best regards, > > Hong Yuan Khor > Flinders University > South Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stanley.Stylli <@t> mh.org.au Sun Nov 9 14:50:07 2003 From: Stanley.Stylli <@t> mh.org.au (Stylli, Stanley) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] PECAM staining on FFPE sections Message-ID: <00519F9F41D2844D9C75BEE1F6AC09AFC89917@rmhmail1.ssg.org.au> Dear All, I am was wondering if anyone has used the following Becton Dickinson antibody Cat No. 550310-Rat CD31 Pure MAB (TLD-3A12) on FFPE rat tissue sections ? What antigen retrieval techniques have you used ? The antibody requires the tissue to be zinc fixed but was wondering if anyone has had any luck on formalin fixed sections ? I have had luck with rat kidney but not much with rat brain. Has anyone used another PECAM antibody that has worked well on FFPE rat brain ? Thankyou in advance. Stan Stylli Stan Stylli Department of Surgery, 5th Floor Clinical Sciences Building Royal Melbourne Hospital University of Melbourne Parkville, Australia, 3052. Tel: 61-3-93427616 Fax:61-3-9347-7695 -------------- next part -------------- A non-text attachment was scrubbed... Name: Stylli, Stanley.vcf Type: text/x-vcard Size: 230 bytes Desc: Stylli, Stanley.vcf Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/2c04dd9e/StylliStanley.vcf From Stanley.Stylli <@t> mh.org.au Sun Nov 9 15:26:36 2003 From: Stanley.Stylli <@t> mh.org.au (Stylli, Stanley) Date: Fri Sep 16 15:22:10 2005 Subject: FW: [Histonet] PECAM staining on FFPE sections Message-ID: <00519F9F41D2844D9C75BEE1F6AC09AFC89918@rmhmail1.ssg.org.au> A lttle clarification....the antibody is a Pharmingen antibody available through Becton Dickinson -----Original Message----- From: Stylli, Stanley Sent: Monday, 10 November 2003 7:50 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PECAM staining on FFPE sections Importance: High Dear All, I am was wondering if anyone has used the following Becton Dickinson antibody Cat No. 550310-Rat CD31 Pure MAB (TLD-3A12) on FFPE rat tissue sections ? What antigen retrieval techniques have you used ? The antibody requires the tissue to be zinc fixed but was wondering if anyone has had any luck on formalin fixed sections ? I have had luck with rat kidney but not much with rat brain. Has anyone used another PECAM antibody that has worked well on FFPE rat brain ? Thankyou in advance. Stan Stylli Stan Stylli Department of Surgery, 5th Floor Clinical Sciences Building Royal Melbourne Hospital University of Melbourne Parkville, Australia, 3052. Tel: 61-3-93427616 Fax:61-3-9347-7695 ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ From Linresearch <@t> aol.com Sun Nov 9 15:38:06 2003 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] (no subject) Message-ID: <19c.1c7c7e5b.2ce00dbe@aol.com> Hi, Donna Montague form the University of Arkansas, I think that you posted a procedure for GFP visualization a while back. I would like to talk with you offline and ask some more detailed quetions concerning the procedure. I would appreciate it if you could e-mail me a means of contacting you personally. Thanks, Lin -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031109/f6a1848c/attachment.htm From Linresearch <@t> aol.com Sun Nov 9 15:41:02 2003 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Fwd: [GFP Message-ID: <33.405c5a58.2ce00e6e@aol.com> -------------- next part -------------- An embedded message was scrubbed... From: Linresearch@aol.com Subject: [Histonet] (no subject) Date: Sun, 9 Nov 2003 16:38:06 EST Size: 4065 Url: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031109/c2c79ef8/attachment.eml From le.paix <@t> verizon.net Sun Nov 9 18:51:35 2003 From: le.paix <@t> verizon.net (Michael F.) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] career change from rehab to histo tech. Message-ID: hello, all i'm new to the list, as the subject indicates i've a M.S. in rehab and have worked in it 10+ years, i've always longed to use my mind a bit more, but am concerned that simply either on the job or a community college certificate and certification to prepare slides might fall short of my desire. I'm being told that I probably would need to get into 'research'. So, I'm curious how feasible this might be. Further, I'm curious about demand for techs. I'm thinking there is likely a demand, however there must be much less positions for techs. than rehab people? I would appreciate your considerate comments in this challenging situation of mine. cheers, M. From d.catmull <@t> latrobe.edu.au Sun Nov 9 22:29:31 2003 From: d.catmull <@t> latrobe.edu.au (Deanne Catmull) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Genetics anyone? In-Reply-To: Message-ID: <3.0.6.32.20031110152931.008f8240@pop.latrobe.edu.au> Hi Cheryl, Diploid is an organism which has two sets of each chromosome, triploid is three copies and tetraploidy is four copies of each chromosome. Hope this helps! Deanne at La Trobe Bundoora. At 07:47 AM 11/6/03 -0600, Cheryl Crowder wrote: >Good morning - This questions was put to me yesterday and, knowing >little about genetics, I'm stumped. Can any of you help me. This >researcher is collecting samples which he says are either diploid, >triploid or tetraploid. He states that someone told him there is a >stain techniques that "would distinguish the 'ploidy' by the intensity >of the stained tissue". > >Have any of you heard of such a thing or know someone I can contact for >this "unusual" question? Thank you, in advance. Cheryl > > >Cheryl Crowder, BA, HTL(ASCP) >Chief Technologist >Anatomic Pathology >Department of Pathobiological Sciences >School of Veterinary Medicine >Louisiana State University >Skip Bertman Drive >Baton Rouge, LA ?70803 > >225-578-9734 >FAX: ?225-578-9720 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Deanne Catmull Neuroimmunology Laboratory Dept of Biochemistry La Trobe University Bundoora Vic 3086 Ph:9479-1155 From d.catmull <@t> latrobe.edu.au Sun Nov 9 22:44:50 2003 From: d.catmull <@t> latrobe.edu.au (Deanne Catmull) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Help on Teared tissues In-Reply-To: Message-ID: <3.0.6.32.20031110154450.00922c90@pop.latrobe.edu.au> Hi Julien, According to my troubleshooting protocol it says that either poor processing of tissue or the wax being too hot during infiltratiuon or embedding is probably your problem. Deanne at La Trobe Uni. At 08:28 PM 11/7/03 +0000, Julien Lambrey de Souza wrote: >Hello all, > >Could someone give me the various reasons why tissues are torn on slides. In >my case I doubt that this is due to the microtome. Is processing in parafin >a possibility? Maybe longer times in 100% alchool, Xylen and then parafin? > >Thanks, >Julien De Souza. > >_________________________________________________________________ >The new MSN 8: advanced junk mail protection and 2 months FREE* >http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.ms n.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Deanne Catmull Neuroimmunology Laboratory Dept of Biochemistry La Trobe University Bundoora Vic 3086 Ph:9479-1155 From jkiernan <@t> uwo.ca Sun Nov 9 23:12:20 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Help on Teared tissues References: <3.0.6.32.20031110154450.00922c90@pop.latrobe.edu.au> Message-ID: <3FAF1E34.BECD2863@uwo.ca> Tears in the sections come from faults on the cutting edge of the microtome's knife. These are easily seen by looking at the blade with a microscope, using the X10 objective. Ask a more experienced colleague to show you how to do this safely. The Safety is partly for your fingers bur MOSTLY for the knife's edge. That's if you use a solid steel knife. With disposable blades your finger may be more valuable, even to your employer's insurance company. If the microscope shows nicks or lesser irregularities in the edge of the blade you won't be able to cut good sections. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ------------ A Histonetter wrote: > Could someone give me the various reasons why > tissues are torn on slides. In my case I > doubt that this is due to the microtome. > > Is processing in paraffin possibility ... From louise_renton <@t> hotmail.com Mon Nov 10 02:58:31 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] What am I going to do? Message-ID: Dear Jenny, this might be absolutely no help to you at all, but I have NEVER had any sucess in keeping my MMA sections on the slide. I have tried Haupt's solution, chrome gelatin (only worked at high concentrations that left ++++background on slide), Superfrost and APES coated slides. So we come back to the old tried and test and , if truth be told, a little iffy, method of staining the sections free - floating ( we use ordinary processing casettes for this) and then mounting them from abs alcohol with Entellan. Of course, with this method, IMP is probably impossible, but tinctorial staining works fine if with a little tweaking. If you want a more detailed method, let me know and I will send you one best rgards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: Jenny Molde To: histonet@pathology.swmed.edu Subject: [Histonet] What am I going to do? Date: Fri, 7 Nov 2003 14:12:16 +0200 Hi everyone out there Yes it`s me again. I have not been able to keep the stented sections on my slides. I have tried all the suggestions made by you wonderful people out there. It`s time to take early retirement as I have no other options left now. Thanks all for your input but none of it worked. Do any of you know if methylmethacrylate goes off for any reason? This is my last resort. Many thanks in advance. Jenny Molde Cardiovascular Research Unit University of Cape Town South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Accept HUGE attachments with MSN Extra Storage! http://join.msn.com/?page=dept/home&pgmarket=en-xe From louise_renton <@t> hotmail.com Mon Nov 10 03:02:26 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] sectioning tissue mircroarrays Message-ID: Dear Jason, Could it be that when the cores were insetrd into the final block they were not warmed sufficiently? I have seen this "shelling" out of normal tissue bocks when there has been a temperature mismatch, ie when the block of tissue was cold and a hardened layer of wax had formed, and then embedded in normal hot wax. The only remedy under these circumstances was to melt the whole thing down and start again. best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "jason madore" To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sectioning tissue mircroarrays Date: Fri, 07 Nov 2003 06:54:18 -0800 Salut everyone, I am hoping that someone can give me pointers for sectioning of tissue microarrays. I am using leica RM2125 and the first array i need to section is a 0.06mm 400core ovarian serous tumour array. I have a problems with the cores rolling up as the section is cut. About half of the roled cores flatten out in the water bath but still losing half the array in this way is not good. Is the knife or some step prior to sectioning. The block was incubated at 37ds for half an hour. Any pointers on dealing with these problems or any other problems I might encounter would be greatly appreciated. Thanks in advance. I am of course checking the histonet archives as well. Ciao, J. _________________________________________________________________ MSN 8 with e-mail virus protection service: 2 months FREE* http://join.msn.com/?page=features/virus&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Defend your inbox - join the fight against spam. http://www.msn.co.za/antispam/ From antje.marcantonio <@t> pharma.novartis.com Mon Nov 10 03:21:57 2003 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] CD 4/CD 8 staining of frozen stomach sections of mice Message-ID: Hello Manuelle may I ask you to check your secondary antibody? How does the control look like without applying the primary ? We use both for CD4 and for CD8a on mouse frozens antibodies( host rat) from Pharmigen. As secondary I use a rabbit anti-rat biotinylated one, mouse adsorbed from Vector. For the blocking of endogenous peroxidase don't use methanol (!) , it weakens the signal in most cases. For the unspecific binding block we use 10% normal rabbit serum (-> secondary host species). I hope this helps. Good luck, Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland Tel. +41 61 324 6730 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/f2d244f1/attachment.htm From histo <@t> skm.org.pk Mon Nov 10 05:01:01 2003 From: histo <@t> skm.org.pk (Histology) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] unsubcribe Message-ID: Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology@skm.org.pk From subratab <@t> bdonline.com Mon Nov 10 05:36:49 2003 From: subratab <@t> bdonline.com (Subratab) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Biochemical test for monocyte/macrophage in tissue homogenate Message-ID: <200311101145.hAABjw76025026@korotoa.bdonline.com> Dear All In relation to the following post I am interested to know if there is any test that can measure monocyte/macrophage in homogenized tissue. I am not talking about cytochemistry/histochemistry method where the tissue section or specimen is on a slide. I need a biochemical method (like colorimetry) (in addition to IHC) to have a measure of macrophage infiltration in tissue. For this purpose I want to test tissue homogenate. Thanks in advance Subrata Biswas Dept of Nephrology UNICAMP, SP, Brazil To: Ramesh Subrahmanyam , histonet@lists.utsouthwestern.edu From: Mark Frei Date: Wed, 5 Nov 2003 13:07:56 -0600 Subject: Re: [Histonet] Distinguishing monocytes from granulocytes This is a multipart message in MIME format. --=_alternative 00694CD586256DD5_= Content-Type: text/plain; charset="us-ascii" Have you thought of cytochemistry? Chloroacetate esterase and naphthyl acetate esterase are two enzymatic assays that will easily and cheaply differentiate the mentioned cell types. From ctsmolde <@t> capeheart.uct.ac.za Mon Nov 10 06:03:14 2003 From: ctsmolde <@t> capeheart.uct.ac.za (Jenny Molde) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Looking for Tiffany Message-ID: Hi all I am looking for Tiffany of the Texas Heart Institute. Apparently she does the same stent work I do. Would really like an e-mail address or work number please. Many thanks. Jenny Molde Cardiovascular Research Unit University of Cape Town South Africa From tissuearray <@t> hotmail.com Mon Nov 10 07:14:08 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] sectioning tissue mircroarrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/abfe934e/attachment.htm From jlambrey <@t> hotmail.com Mon Nov 10 08:04:13 2003 From: jlambrey <@t> hotmail.com (Julien Lambrey de Souza) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] Torn tissues followup Message-ID: Hello to all histonetters, I am having problems with torn tissues and I have already posted a message asking what may be the causes. Thanks for the various replies. Most of you aksed me more details about the procedure in order to try and define a problem. so here goes: Small fish (5cm) where fixed in formalin and then preserved in 70% EtOh Processing is done on an automated processor 2 hours in 95%alcohol 2 hours in 100% 3 hours in xylene 1 hour in wax (paraplus) 1 hour in wax with vacuum. Embedding is done with paraplus wax on a histocenter II. Sectioning is done at 7 microns, transversally to body length. To mount secions on slides, 2 methods are used. The first is a heated waterbath to unrinkle the tape. But this methods complicates recognition of sections to the fish length. That's why we prefer to lay the tapes on a black cardboard paper, put some water on slides, put the tape on the slide and then heat slightly the slide on a slide warmer so the tape expands to unrinkle on the buble of water. This technique has worked fine up to now and enables us to keep track of how far we are in the fishe's body. Staining is done on an automated stainer Xylene 3 min (x3) 100% alcohol 2 min 100% 3 min 95% 2 min (x2) 70% 2 min Water 2 min Hematoxylin 4 min Water 2 min Bluing reagent 2 min Water 3 min 95% 30 sec Eosine 2 min 95% 1 min (x2) 100% 1 min 100% 2 min Xylene 3 min (x3) Coverslips are mounted automatically. So there it is. I don't think the tearing is due to microtome blade since I use a new disposable blade each time I have a doubt. So, according to the comments we received up to now, the parameters I'm going to change are the processing times in alcohol and xylene. I am going to lower them but raise the time in parafin. I have to check the parafin temperature as I was told an excessively hot wax will not penetrate as well. Also I'm going to try and reduce slide warming temperature but leave them dry longer, since wet sections may slide off during staining. So, if you have any further suggestion to help me solve my problem, all comments are welcome. Chears, Julien De souza. _________________________________________________________________ Add photos to your messages with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From sladd <@t> hsc.usf.edu Mon Nov 10 08:05:27 2003 From: sladd <@t> hsc.usf.edu (Sharron Ladd) Date: Fri Sep 16 15:22:10 2005 Subject: [Histonet] found reference for 10% KOH treatment of nails Message-ID: <3FAF9B27.5080305@hsc.usf.edu> Dear Histonet, I searched the archives for references regarding softening nails with 10% KOH prior to processing and I didn't find anything. I did find that there were several people looking for a reference as well, so I thought I would share the one I found: Lewin K, DeWit SA, Lawson R. Softening techniques for nail biopsies. Arch Dermatol. 1973 Feb;107(2):223-4. They had the article in our library, so I have a copy. The authors tried many methods of softening and then rated ease of sectioning in a nice little table. Sharron University of South Florida From juan.gutierrez <@t> christushealth.org Mon Nov 10 08:15:18 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Looking for Tiffany Message-ID: Tiffany.L.Sheffield@uth.tmc.edu -----Original Message----- From: Jenny Molde [mailto:ctsmolde@capeheart.uct.ac.za] Sent: Mon 11/10/2003 6:03 AM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] Looking for Tiffany Hi all I am looking for Tiffany of the Texas Heart Institute. Apparently she does the same stent work I do. Would really like an e-mail address or work number please. Many thanks. Jenny Molde Cardiovascular Research Unit University of Cape Town South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tiffany.L.Sheffield <@t> uth.tmc.edu Mon Nov 10 08:21:48 2003 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Tiffany L Sheffield) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Looking for Tiffany References: Message-ID: <3FAF9EFC.5926B9A4@uth.tmc.edu> Hi Jenny, I actually work for UT-Houston Medical School. Feel free to contact me anytime! Jenny Molde wrote: > Hi all > I am looking for Tiffany of the Texas Heart Institute. Apparently she > does the same stent work I do. Would really like an e-mail address or > work number please. Many thanks. > > Jenny Molde > Cardiovascular Research Unit > University of Cape Town > South Africa > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- A non-text attachment was scrubbed... Name: Tiffany.L.Sheffield.vcf Type: text/x-vcard Size: 374 bytes Desc: Card for Tiffany Sheffield-Lopez Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/da06a06d/Tiffany.L.Sheffield.vcf From mcauliff <@t> umdnj.edu Mon Nov 10 08:55:56 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] URGENT! Questions regarding snap freezing in liquid nitrogen with isopentene In-Reply-To: <1068295932.3face6fc662f2@imap.flinders.edu.au> References: <1068295932.3face6fc662f2@imap.flinders.edu.au> Message-ID: <3FAFA6FC.7010807@umdnj.edu> I learned to snap freeze with 2-methylbutane (isopentane) cooled by liquid nitrogen over 25 years ago and it was a common proceedure then. Try textbooks on histological technique for the original references. Geoff khor0011@flinders.edu.au wrote: >Hi everyone, > >I am an Honours student, currently finishing up my Honours thesis. >I am wondering if anyone could give me some REFERENCES on snap freezing mouse >tissues using liquid nitrogen and ISOPENTENE. I have used liquid nitrogen ONLY >for snap freezing my mouse tissues in my project, but not with isopentene. And >i have been having cryostat sectioned mouse tissues that have a lot of tissue >degeneration, as well as artefacts after fixing in 2% para & 0.5% Glu, and i >got the idea from someone that this maybe due to the fact that i did not use >isopentene in the snap freezing process. > >So what i want is to discuss this in my thesis. So it would be great if >someone could give me any scientific references/comments/advice on this method >whereby isopentene is used in the snap freezing process, and why using this is >important. > >I have been doing some internet searching myself, but any advice or help from >all histo experts out there would be greatly appreciated. > >Best regards, > >Hong Yuan Khor >Flinders University >South Australia > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From JNocito <@t> Pathreflab.com Mon Nov 10 09:14:10 2003 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Billing Surepath for Digene HPV, GC/CT Message-ID: I know this isn't really histology related but I need expert help. We started performing the Digene HPV Hybrid Capture2 along with their GC/CT testing. We also perform these tests using Cytyc's Thinprep and Tripath Imaging Surepath vials. I know the FDA approved the Digene testing off the Thinprep vial, but when it comes to the Surepath, can we bill Medicaid and Medicare for these tests performed on Surepath? As Always, thanks for your input. Joe Nocito, BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab From mcauliff <@t> umdnj.edu Mon Nov 10 10:07:00 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] immunofluorescence problem In-Reply-To: <4f32df4f34de.4f34de4f32df@shaw.ca> References: <4f32df4f34de.4f34de4f32df@shaw.ca> Message-ID: <3FAFB7A4.20808@umdnj.edu> Hi Stephanie: Have you compared a NeuN to an adjacent section NeuN that was preceeded by HCl denaturation to see if the HCl really is the problem? Can you use a fixative that does not require DNA denaturation or requires a milder treatement? Can you do the NeuN, photograph the results, then do the other stains and photograph them? A final composite with all 3 antibodies can be made in PhotoShop. Geoff slieblich@shaw.ca wrote: >I am doing triple labeling immuno fluorescence of mouse brain tissue and am having some difficulties. My three markers are NeuN, GFAP, and BrdU. Previously when I have triple labeled, I only get gfap and brdu fluorescence and no NeuN (or rarely and inconsistently). I have found out that it is possible that the acid used to denature the DNA for BrdU may be interfering with NeuN antibodies. I was wondering if anyone has also had this problem with NeuN. Does anyone have any ideas to get around the NeuN/hydrochloric acid issue? > >Thanks! > >Stephanie Lieblich >Cellular Neurophysiology Lab >University of British Columbia >Vancouver, B.C., Canada > > > > -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From settembr <@t> umdnj.edu Mon Nov 10 10:38:39 2003 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Amyloid Precursor Protein Message-ID: Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human tissue? I have NovoCastra's monoclonal antibody. I have used Alzheimer's disease brain tissue that works with other antibodies. I use a very good Target Retreival solution from DakoCytomation in a steamer for 40 minutes. I have used it at the recommended dilutions and I have used them more potent than that too. Our neuropathologist tells me that it has been in use regularly for some time now. HELP! Dana Settembre Immunohistochemistry Lab Unversity Hospital-UMDNJ Newark, NJ USA From ploykasek <@t> phenopath.com Mon Nov 10 10:49:38 2003 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Job opening Message-ID: I would like to announce an opening at Phenopath Laboratories in Seattle, WA. If you would like further information, please contact me or Sheri Storey. Thanks. Patti Loykasek IMMUNOHISTOCHEMISTRY TECHNOLOGIST PhenoPath Laboratories, a pathology reference laboratory, has an opportunity in the clinical immunohistochemistry division for a full-time technologist, day shift. We are in a state-of-the-art facility located on the scenic Ship Canal in the eclectic Fremont neighborhood of Seattle, WA. Job Description: Responsibilities may include tissue sectioning (paraffin and frozen), and performing immunohistochemistry, immunofluorescence, and molecular testing on patient samples. Participation in the development of new tests or technologies, as well as participation in clinical research projects are also included. Quality control, safety, and preventive maintenance procedures and documentation are required elements of this position as well Required Skills/Experience: Strong preference given to ASCP certified (or certification-eligible) laboratory techs. Trained laboratory techs of any discipline are encouraged to apply (histotech, med tech, cytotech?..). PhenoPath Laboratories is committed to hiring the best person for the job. PhenoPath Laboratories is a national specialty pathology laboratory with 5 pathologists and 13 technologists committed to providing outstanding clinical and research services. We offer an excellent compensation and benefits package, including 401k. To apply, please contact: Sheri Storey Director of Human Resources PhenoPath Laboratories 551 N. 34th St., Suite 100 Seattle, WA 98103 phone: 206-374-9000 fax: 206-374-9009 e-mail: sheri@phenopath.com From Rcartun <@t> harthosp.org Mon Nov 10 11:17:01 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Amyloid Precursor Protein Message-ID: We use Chemicon's MAB348 at 1:5,000 (overnight incubation) with DakoCytomation's EnVision+/DAB+ detection. Retrieval is performed in a 96 degree waterbath for 40 minutes. Richard Cartun >>> Dana Settembre 11/10/03 11:38AM >>> Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human tissue? I have NovoCastra's monoclonal antibody. I have used Alzheimer's disease brain tissue that works with other antibodies. I use a very good Target Retreival solution from DakoCytomation in a steamer for 40 minutes. I have used it at the recommended dilutions and I have used them more potent than that too. Our neuropathologist tells me that it has been in use regularly for some time now. HELP! Dana Settembre Immunohistochemistry Lab Unversity Hospital-UMDNJ Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sa.drew <@t> hosp.wisc.edu Mon Nov 10 11:36:49 2003 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Amyloid Precursor Protein Message-ID: We are using Chemicon's cat#MAB348 at a 1:200 after pressure cooker HIER in EDTA buffer... This is run on our Ventana stainers for only an 8 min. titration. Sally Ann Drew-MT(ASCP) Univ. of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. VAH-DM223 Madison, WI 53792-2472 608-265-6596 sa.drew@hosp.wisc.edu -----Original Message----- From: Dana Settembre [mailto:settembr@umdnj.edu] Sent: Monday, November 10, 2003 10:39 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Amyloid Precursor Protein Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human tissue? I have NovoCastra's monoclonal antibody. I have used Alzheimer's disease brain tissue that works with other antibodies. I use a very good Target Retreival solution from DakoCytomation in a steamer for 40 minutes. I have used it at the recommended dilutions and I have used them more potent than that too. Our neuropathologist tells me that it has been in use regularly for some time now. HELP! Dana Settembre Immunohistochemistry Lab Unversity Hospital-UMDNJ Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcdonal1 <@t> ccf.org Mon Nov 10 11:37:51 2003 From: mcdonal1 <@t> ccf.org (Linda McDonald) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Formalin pigment Message-ID: Got a call today from another lab using 10% alcoholic formalin on their processors. They have used it for many years. They now are getting formalin pigment. Is anyone else experiencing formalin pigment with 10% alcoholic formalin? Not sure whar they are fixing in before the cassettes are placed on the processors, maybe 10% NBF. Any ideas would be great. Thanks, LGMc ------------------------------------------------------------------------------ Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ============================================================================== From t-kuzniar <@t> md.northwestern.edu Mon Nov 10 11:58:05 2003 From: t-kuzniar <@t> md.northwestern.edu (t-kuzniar@md.northwestern.edu) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] B-galactosidase in lung Message-ID: <200311101758.hAAHwJIf029319@casbah.it.northwestern.edu> Dear all, I am still working on my b-galactosidase in lung tissue, still without major success. So far, I have tried: - X-gal reaction on frozen sections, with 0.2% glutaraldehyde fixation (10 minutes), and detergent washes (protocol below), with or without the sucrose cryoprotection – I had no signal in the tissue, even though I know the b-gal is there. - X-gal reaction on frozen sections, fixed in 100% acetone, with PBS washes – no signal - ICH (I use Rabbit IgG alkaline phosphatase VECTASTAIN system) for b-gal on paraffin sections, using the polyclonal Ab from Chemicon (has anybody used it and have comments on it?) – in 0.2% glutaraldehyde, with or without sucrose cryoprotection step – there was a non-specific staining of the primary antibody (my “no primary antibody” controls were negative, but my naïve animals that SHOULD NOT have b-gal had an intense staining from this polyclonal Ab) - ICH for b-gal on frozen sections, with the same antibody and with either 0.2% glutaraldehyde or 100% acetone fixation – I had an intense staining of all the controls in my biotin-avidin system. Protocol for X-gal staining Sacrifice an animal by injecting an overdose of Nembutal (more than 60 mg/kg) ip Create a pneumothorax and remove en bloc trachea and heart-lungs. Keep everything on ice. Fill lungs via a cannula inserted into the trachea with ice cold PBS (x2) Slice lobes by halves, in case of the left lobe – slice in four Cryoprotect with 15% sucrose in PBS for 1 hour at 4oC Cryoprotect with 30% sucrose in PBS overnight at 4oC Cover samples with Tissue-TEK Freeze in -80oC for >1 hour Cut frozen sections. Fix in 0.2% glutaraldehyde, 5mM EGTA, 2mM MgCl2 in PBS on ice for 10 minutes Wash in 0.01% sodium deoxycholate, 0.02% Triton X-100, 2 mM MgCl2 in PBS on ice for 10 minutes (x2). Wash in 0.01% sodium deoxycholate, 0.02% Triton X-100, 2 mM MgCl2 in PBS at RT for 10 minutes (x2). Incubate overnight with lacZ staining solution at 37oC protected from light. Wash twice in PBS plus 2 mM MgCl2 at room temperature for 5 minutes each Rinse in distilled water Counterstain for 30 seconds in Nuclear Fast Red Wash three times in distilled water Dehydrate through ethanol (5 minutes each in 50%, 70%, and 100% ethanol) Clear sections twice for 5 minutes each in xylene Mount Thanks, Tom Kuzniar From jcox90 <@t> yahoo.com Mon Nov 10 12:04:51 2003 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Mopec MB600 gross station Message-ID: <20031110180451.58964.qmail@web40909.mail.yahoo.com> Our lab purchased a Mopec grossing station a few months ago. It is the self contained model MB600. We are having a problem with smelling formalin fumes even with brand new filters. All we gross is small biopsies and the lids are put back on the bottles when done and the formalin covered. Is anyone having problems with this model or is this just a fluke. This is the first self contained unit I have ever used. Thanks in advance Jill Cox HT (ASCP) Seattle Histology Lab --------------------------------- Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/5288dce1/attachment.htm From cmconway <@t> usgs.gov Mon Nov 10 12:51:44 2003 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] service for LX-120 tissue processor? Message-ID: We are looking for someone (preferably in the Seattle, WA area) who can provide service/preventive maintenance for our 9 year old LX-120 tissue processor. Fisher Scentific had been providing service for this equipment. Any help would be appreciated. Sincerely, Carla Conway Western Fisheries Research Center 6505 NE 65th St. Seattle, WA 98115 ph: 206-526-6282 ext,. 242 fax: 206-526-6654 From kgrobert <@t> rci.rutgers.edu Mon Nov 10 13:38:40 2003 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] service for LX-120 tissue processor? In-Reply-To: References: Message-ID: <3FAFE940.5020006@rci.rutgers.edu> Carla- We had the same problem not too long ago-looks like Fisher gave the machine to Hacker, Inc., so you can likely contact them for service. http://www.hackerinstruments.com/lx120.htm Good luck- Kathleen Roberts Principal Lab Technician Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 Carla M Conway wrote: >We are looking for someone (preferably in the Seattle, WA area) who can >provide service/preventive maintenance for our 9 year old LX-120 tissue >processor. Fisher Scentific had been providing service for this equipment. >Any help would be appreciated. > >Sincerely, > >Carla Conway > >Western Fisheries Research Center >6505 NE 65th St. >Seattle, WA 98115 >ph: 206-526-6282 ext,. 242 >fax: 206-526-6654 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ranahay <@t> fastmail.fm Mon Nov 10 14:36:35 2003 From: ranahay <@t> fastmail.fm (Rana Hay) Date: Fri Sep 16 15:22:11 2005 Subject: Fwd: [Histonet] unsubcribe Message-ID: <20031110203635.3661640F90@server1.messagingengine.com> Rana Hay -- Rana Hay ranahay@fastmail.fm -- http://www.fastmail.fm - A no graphics, no pop-ups email service From MElliott <@t> mrl.ubc.ca Mon Nov 10 14:37:05 2003 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Antibody to E-selectin Message-ID: Hi I have a reseacher here looking for a specific clone to E-selectin (clone 14G2). I can't locate it anywhere in any of my catalogies and have gone through Serotec's antibody search feature, but they also are unable to locate a source. Anybody knnow where I could get it? Thanks Mark Elliott Research Associate UBC-McDonald Research Lab-iCAPTURE Centre St. Paul's Hospital Vancouver, BC Canada From p_bourne_14526 <@t> yahoo.com Mon Nov 10 15:55:38 2003 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] IC5 antibody Message-ID: <20031110215538.23506.qmail@web10007.mail.yahoo.com> Greetings everyone: Does anyone know about an antibody for IC5? Should be used in adenoca of the uterus vx cervix. Thanks in advance. Pat --------------------------------- Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/19639908/attachment.htm From weneng <@t> hotmail.com Mon Nov 10 18:51:19 2003 From: weneng <@t> hotmail.com (Wendy England) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Perls Iron Stain control slides Message-ID: Hello, I was asked to do this staining on mouse spleen and liver samples. This is my first time to do this staining. I need a positive control slide that doesn't contain too much iron. Could anybody tell me what control slide I should use and/or where I can buy it? Thanks in advance. Wendy _________________________________________________________________ Great deals on high-speed Internet access as low as $26.95. https://broadband.msn.com (Prices may vary by service area.) From Linresearch <@t> aol.com Mon Nov 10 19:33:28 2003 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] GFP Message-ID: <148.1c39f5aa.2ce19668@aol.com> Hello, I need to use this procedure to visualize a vector that will be injected into rat duodenum. I would appreciate any hints on fixation and staining procedures procedure that anyone may want to share. lin -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031110/fa4a1bd8/attachment.htm From jim.manavis <@t> imvs.sa.gov.au Mon Nov 10 19:38:20 2003 From: jim.manavis <@t> imvs.sa.gov.au (jim) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Picric Acid Message-ID: <002601c3a7f4$7c9b09a0$7a6c140a@imvs.sa.gov.au> Has anyone information or data on the safe disposal of Picric Acid (crystals have formed on lid of bottle). Thanks Jim Manavis Senior Hospital Scientist Department of Neuropathology Institute of Medical & Veterinary Science Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 FAX: 61-08-8222 3204 e.mail: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031111/8d311a4e/attachment.htm From jkiernan <@t> uwo.ca Mon Nov 10 23:11:36 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Picric Acid References: <002601c3a7f4$7c9b09a0$7a6c140a@imvs.sa.gov.au> Message-ID: <3FB06F88.2C74A763@uwo.ca> Wash under a tap. Put some water in the jar if it has dried out. Picric acid explodes if its temperature goes above 300C. Sources: Lange's Handbook of Chemistry, just checked; also remembered from various textbooks read over the years. This cannot happen in the presence of liquid water. Nearly all anecdotes about picric acid explosions in labs are urban legends (= untrue). For confirmation of this assertion, check it out with Google, but allow a couple of hours and use your brain to evaluate the produce. __________________________________ > jim wrote: > > Has anyone information or data on the safe disposal > of Picric Acid (crystals have formed on lid of > bottle). > > Thanks > > Jim Manavis > Senior Hospital Scientist > Department of Neuropathology > Institute of Medical & Veterinary Science > Adelaide, SA, 5000 > Australia > Phone: 61-08-8222-3668 > FAX: 61-08-8222 3204 > e.mail: jim.manavis@imvs.sa.gov.au > Disclaimer: Not this little black duck! > -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From Laurie.Reilly <@t> jcu.edu.au Mon Nov 10 23:34:41 2003 From: Laurie.Reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Torn tissues followup In-Reply-To: Message-ID: <5.1.0.14.0.20031111152436.00a2a5f0@mail.jcu.edu.au> Julien, You haven't mentioned anything about decalcifying these fish. When we are to section fish, they are usually fixed in Davidson's or Bouin's, both of which contain acids which will decalcify the bones in the fish. Maybe your torn tissues are caused by the calcified bones damaging your microtome knife. Regards, Laurie. At 02:04 PM 11/10/03 +0000, Julien Lambrey de Souza wrote: >Hello to all histonetters, > >I am having problems with torn tissues and I have already posted a message >asking what may be the causes. Thanks for the various replies. Most of you >aksed me more details about the procedure in order to try and define a >problem. so here goes: > >Small fish (5cm) where fixed in formalin and then preserved in 70% EtOh > >Processing is done on an automated processor >2 hours in 95%alcohol >2 hours in 100% >3 hours in xylene >1 hour in wax (paraplus) >1 hour in wax with vacuum. > >Embedding is done with paraplus wax on a histocenter II. > >Sectioning is done at 7 microns, transversally to body length. > >To mount secions on slides, 2 methods are used. The first is a heated >waterbath to unrinkle the tape. But this methods complicates recognition >of sections to the fish length. That's why we prefer to lay the tapes on >a black cardboard paper, put some water on slides, put the tape on the >slide and then heat slightly the slide on a slide warmer so the tape >expands to unrinkle on the buble of water. This technique has worked fine >up to now and enables us to keep track of how far we are in the fishe's body. > >Staining is done on an automated stainer >Xylene 3 min (x3) >100% alcohol 2 min >100% 3 min >95% 2 min (x2) >70% 2 min >Water 2 min >Hematoxylin 4 min >Water 2 min >Bluing reagent 2 min >Water 3 min >95% 30 sec >Eosine 2 min >95% 1 min (x2) >100% 1 min >100% 2 min >Xylene 3 min (x3) > >Coverslips are mounted automatically. > >So there it is. I don't think the tearing is due to microtome blade since >I use a new disposable blade each time I have a doubt. >So, according to the comments we received up to now, the parameters I'm >going to change are the processing times in alcohol and xylene. I am going >to lower them but raise the time in parafin. I have to check the parafin >temperature as I was told an excessively hot wax will not penetrate as well. > >Also I'm going to try and reduce slide warming temperature but leave them >dry longer, since wet sections may slide off during staining. > >So, if you have any further suggestion to help me solve my problem, all >comments are welcome. > >Chears, > >Julien De souza. > >_________________________________________________________________ >Add photos to your messages with MSN 8. Get 2 months FREE*. >http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly Ph 07 4781 4468 Physiology & Pharmacology Fax 07 4779 1526 Aust.Inst.of Tropical Vet.& Animal Sc. James Cook University Townsville Qld. 4811 laurie.reilly@jcu.edu.au Australia. From abright <@t> brightinstruments.com Tue Nov 11 02:42:00 2003 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Torn tissues followup Message-ID: Julien, I agree with Laurie, but as an alternative, a tungsten carbide tipped microtome would overcome this problem too. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Laurie Reilly [mailto:Laurie.Reilly@jcu.edu.au] Sent: 11 November 2003 05:35 To: Julien Lambrey de Souza; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Torn tissues followup Julien, You haven't mentioned anything about decalcifying these fish. When we are to section fish, they are usually fixed in Davidson's or Bouin's, both of which contain acids which will decalcify the bones in the fish. Maybe your torn tissues are caused by the calcified bones damaging your microtome knife. Regards, Laurie. At 02:04 PM 11/10/03 +0000, Julien Lambrey de Souza wrote: >Hello to all histonetters, > >I am having problems with torn tissues and I have already posted a >message >asking what may be the causes. Thanks for the various replies. Most of you >aksed me more details about the procedure in order to try and define a >problem. so here goes: > >Small fish (5cm) where fixed in formalin and then preserved in 70% EtOh > >Processing is done on an automated processor >2 hours in 95%alcohol >2 hours in 100% >3 hours in xylene >1 hour in wax (paraplus) >1 hour in wax with vacuum. > >Embedding is done with paraplus wax on a histocenter II. > >Sectioning is done at 7 microns, transversally to body length. > >To mount secions on slides, 2 methods are used. The first is a heated >waterbath to unrinkle the tape. But this methods complicates recognition >of sections to the fish length. That's why we prefer to lay the tapes on >a black cardboard paper, put some water on slides, put the tape on the >slide and then heat slightly the slide on a slide warmer so the tape >expands to unrinkle on the buble of water. This technique has worked fine >up to now and enables us to keep track of how far we are in the fishe's body. > >Staining is done on an automated stainer >Xylene 3 min (x3) >100% alcohol 2 min >100% 3 min >95% 2 min (x2) >70% 2 min >Water 2 min >Hematoxylin 4 min >Water 2 min >Bluing reagent 2 min >Water 3 min >95% 30 sec >Eosine 2 min >95% 1 min (x2) >100% 1 min >100% 2 min >Xylene 3 min (x3) > >Coverslips are mounted automatically. > >So there it is. I don't think the tearing is due to microtome blade >since >I use a new disposable blade each time I have a doubt. >So, according to the comments we received up to now, the parameters I'm >going to change are the processing times in alcohol and xylene. I am going >to lower them but raise the time in parafin. I have to check the parafin >temperature as I was told an excessively hot wax will not penetrate as well. > >Also I'm going to try and reduce slide warming temperature but leave >them >dry longer, since wet sections may slide off during staining. > >So, if you have any further suggestion to help me solve my problem, all >comments are welcome. > >Chears, > >Julien De souza. > From anna <@t> novonordisk.com Tue Nov 11 03:35:21 2003 From: anna <@t> novonordisk.com (ANNA (Annette Enggaard)) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Unsubscribe Message-ID: <267DD1034F2988418DE27613376C8DC50122BDD8@exdkba023.novo.dk> I would like to unsubscribe to this list regards Annette Enggaard -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: 5. november 2003 19:00 To: histonet@lists.utsouthwestern.edu Subject: Histonet digest, Vol 1 #117 - 15 msgs Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-admin@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Improved Eosin (edmondsj) 2. RE: couple of questions (Subject line) (Marshall Terry Dr, Consultant Histopathologist) 3. RE: RE: Looking for a lab to cut stents (Bernard Ian R SSgt 59 CRES/MSROP) 4. Re: RE: Looking for a lab to cut stents (Tiffany L Sheffield) 5. dystrophin (Mcleod) 6. RE: disposal of tissue (Horn, Hazel V) 7. RE: disposal of tissue - Formalin Neutralization (Kelly Booher) 8. RE: disposal of tissue (Claye Clyatt) 9. RE: disposal of tissue (Laurie Colbert) 10. Somebody that can do IHC for us??? (NIDAL E MUVARAK) 11. Re: Histonet digest, Vol 1 #116 - 24 msgs (Instrumedics) 12. Re: Histonet digest, Vol 1 #116 - 24 msgs (Instrumedics) 13. RE: couple of questions (Marion Hiles) 14. Re: Histonet digest, Vol 1 #116 - 24 msgs (Thom Jensen) 15. Distinguishing monocytes from granulocytes (Ramesh Subrahmanyam) --__--__-- Message: 1 Date: Wed, 05 Nov 2003 08:51:50 -0500 From: edmondsj To: John Difford , Histonet Subject: Re: [Histonet] Improved Eosin John, I have a Johns Hopkins eosin recipe, it is as follows: Ingredients: Eosin Y 18gms Phloxine B 7.5gms Biebrich Scarlet 1.5gms Distilled Water 2850 ml Absolute Alcohol 750 ml Procedure: 1: Dissolve all the dyes in the distilled water. 2: Add the absolute alcohol and continue stirring for at least 1 hour. This will make up a volume of 3610 ml, I add about two or three drops of glacial acetic acid to a 500 ml volume before staining tissue (this enhances the stain), you may not like the intensity but you can omit the acetic acid if you like. I hope this is helpful to you. Joyce Edmonds Research Specialist Medical University of SC Charleston, SC --On Tuesday, November 04, 2003 5:02 PM -0500 John Difford wrote: > > Dear All, > > Does anyone remember a formula for an improved Eosin solution which > was displayed on Histonet several years ago? > > It was said to have orginated at Johns Hopkins and contained some > other red dye in addition to eosin. > > I want to make up some more because it was very good for frozen > sections, but I have lost the instructions! > > Many thanks > > John Difford > Histopathology Dept > Royal Free Hospital > London > England, UK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 2 Date: Wed, 5 Nov 2003 14:02:27 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" To: "Marshall Terry Dr, Consultant Histopathologist" , , Subject: RE: [Histonet] couple of questions (Subject line) A more garbled incoherent load of rubbish is hard to imagine. Who is this T Marshall anyway? Sorry, tried to do 2 things at once. Wrong sex. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist=20 Sent: 05 November 2003 13:45 To: jkiernan@uwo.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] couple of questions (Subject line) Received 2 days ago: Re: [Histonet] (no subject) "You, a (? new) histonetter sent a message with=20 the header: couple of questions Grrrr! =20 The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. " Rremeber who sent it John:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: 05 November 2003 06:10 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] couple of questions (Subject line) Dear ********* You, a (? new) histonetter sent a message with=20 the header: couple of questions Grrrr! =20 The Subject line is the most important part of every email in these days of 2 much XS spam. PLEASE put a few specific words in the Subject line. Your email asked two questions (another not good thing). For best replies, try sending two Histonet queries, with=20 different Subject lines such as: Hoovering the cryostat safely Solvents and the VIP processor I've obscured your identity in this Histonet reply because It's not intended to be "flaming." There are lots of emails with no-good subject lines. I'm just trying to ask everyone to send a pertinent Subject line above every=20 message. We all get lots of junk email, and we delete most of the junk messages on the basis of a few word in the Subject line. Herb and Linda=20 are doing a SUPERB job protecting Histonet subscribers from all the viagra adverts etc. =20 --=20 ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ______________________________ She Who Shall Not be Named wrote: >=20 > Hi Everyone, > I have a few questions, > 1. Is anyone using a "home" type vacuum for the > cryostat. Of course the bags would go in the medical > waste. > 2. Is Alcoholic formalin OK to put on the VIP tissue processor, there >is no acetic acid in the solution. =20 > I appreciate all your help. _______________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 3 From: Bernard Ian R SSgt 59 CRES/MSROP To: "'Gordon Grant'" , histonet@lists.utsouthwestern.edu Date: Wed, 5 Nov 2003 08:07:24 -0600 Subject: RE: [Histonet] RE: Looking for a lab to cut stents Cathy MAYton in Arizona Does this. -----Original Message----- From: Gordon Grant [mailto:grantgd@comcast.net] Sent: Tuesday, November 04, 2003 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Looking for a lab to cut stents I was wondering if someone out there can give me a recommendation for a lab which can provide good quality sections of implanted stents. Specifically, we have been told the FDA likes the thick saw cut, ground and polished sections rather than microtomed sections. Thanks Gordon Grant -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, November 02, 2003 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet digest, Vol 1 #113 - 2 msgs Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-admin@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. appropriate pay for grossing techs (Jen Steinberg) 2. Re: appropriate pay for grossing techs (Gudrun Lang) -- __--__-- Message: 1 Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST) From: Jen Steinberg To: histonet@lists.utsouthwestern.edu Subject: [Histonet] appropriate pay for grossing techs --0-1595893577-1067722359=:57919 Content-Type: text/plain; charset=us-ascii Hi, I'm hoping someone can help me. I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers). Would the pay be any different if the employee had an M.S. The position is entry-level. Thanks very much. --------------------------------- Do you Yahoo!? Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919 Content-Type: text/html; charset=us-ascii
Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate starting wage of a grossing tech would be for a private path lab which works mainly on small skin, gyn, and oral specimens and processes about 250 cases a day (between 2 grossers).  Would the pay be any different if the employee had an M.S.  The position is entry-level.  Thanks very much. 
 
 

Do you Yahoo!?
Exclusive Video Premiere - Britney Spears --0-1595893577-1067722359=:57919-- -- __--__-- Message: 2 From: "Gudrun Lang" To: "Histonetliste" , "Jen Steinberg" Date: Sun, 2 Nov 2003 13:19:09 +0100 Subject: Re: [Histonet] appropriate pay for grossing techs This is a multi-part message in MIME format. ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hi Jen, I dont have an answer for you, but another question. In Austria the = person, who does crossing, is a pathologist (m.d.).=20 We have several sorts of tissue f(rom toe to sculp) and I think without = a medical uni-study, it is very difficult. Even our doctors sometimes = make mistakes and look over important details. Is it usual in USA, that histotechs do the crossing? best wishes Gudrun Lang general hospital Linz, Austria ----- Original Message -----=20 From: Jen Steinberg=20 To: histonet@lists.utsouthwestern.edu=20 Sent: Saturday, November 01, 2003 10:32 PM Subject: [Histonet] appropriate pay for grossing techs Hi, I'm hoping someone can help me. I was wondering what the = appropriate starting wage of a grossing tech would be for a private path = lab which works mainly on small skin, gyn, and oral specimens and = processes about 250 cases a day (between 2 grossers). Would the pay be = any different if the employee had an M.S. The position is entry-level. = Thanks very much. =20 ------------------------------------------------------------------------ -= ----- Do you Yahoo!? Exclusive Video Premiere - Britney Spears ------=_NextPart_000_001B_01C3A143.E6310980 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hi Jen,
I dont have an answer for you, but = another=20 question. In Austria the person, who does crossing, is a pathologist = (m.d.).=20
We have several sorts of tissue f(rom = toe to sculp)=20 and I think without a medical uni-study, it is very difficult. Even our = doctors=20 sometimes make mistakes and look over important details.
Is it usual in USA,  that = histotechs do=20 the crossing?
 
best wishes
Gudrun Lang
general hospital Linz, = Austria
----- Original Message -----
From:=20 Jen=20 Steinberg
To: histonet@lists.utsouth w= estern.edu=20
Sent: Saturday, November 01, = 2003 10:32=20 PM
Subject: [Histonet] appropriate = pay for=20 grossing techs

Hi,
   I'm hoping someone can help me.  I was = wondering what=20 the appropriate starting wage of a grossing tech would be for a = private path=20 lab which works mainly on small skin, gyn, and oral specimens and = processes=20 about 250 cases a day (between 2 grossers).  Would the pay be any = different if the employee had an M.S.  The position is = entry-level. =20 Thanks very much. 
 
 

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Exclusive Video Premiere - Britney=20 Spears ------=_NextPart_000_001B_01C3A143.E6310980-- -- __--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 4 Date: Wed, 05 Nov 2003 08:14:38 -0600 From: "Tiffany L Sheffield" To: Bernard Ian R SSgt 59 CRES/MSROP CC: 'Gordon Grant' , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Looking for a lab to cut stents This is a multi-part message in MIME format. --------------2B9EB8314574D12854D86640 Content-Type: multipart/alternative; boundary="------------4B5728D5400BFA6100977CDE" --------------4B5728D5400BFA6100977CDE Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit My lab does stents as well in Houston! We work with Texas Heart Institute in conjunction with other companies on a regular basis. We do thick saw cut ground and polished sections. Hope this helps! If you have any further questions feel free to contact me. Tiffany:) Bernard Ian R SSgt 59 CRES/MSROP wrote: > Cathy MAYton in Arizona Does this. > > -----Original Message----- > From: Gordon Grant [mailto:grantgd@comcast.net] > Sent: Tuesday, November 04, 2003 5:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Looking for a lab to cut stents > > I was wondering if someone out there can give me a recommendation for > a lab which can provide good quality sections of implanted stents. > Specifically, we have been told the FDA likes the thick saw cut, > ground and polished sections rather than microtomed sections. > > Thanks Gordon Grant > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Sunday, November 02, 2003 10:00 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet digest, Vol 1 #113 - 2 msgs > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-admin@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > 1. appropriate pay for grossing techs (Jen Steinberg) > 2. Re: appropriate pay for grossing techs (Gudrun Lang) > > -- __--__-- > > Message: 1 > Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST) > From: Jen Steinberg > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] appropriate pay for grossing techs > > --0-1595893577-1067722359=:57919 > Content-Type: text/plain; charset=us-ascii > > Hi, > I'm hoping someone can help me. I was wondering what the > appropriate starting wage of a grossing tech would be for a private > path lab which works mainly on small skin, gyn, and oral specimens and > processes about 250 cases a day (between 2 grossers). Would the pay > be any different if the employee had an M.S. The position is > entry-level. Thanks very much. > > > > --------------------------------- > Do you Yahoo!? > Exclusive Video Premiere - Britney Spears > --0-1595893577-1067722359=:57919 > Content-Type: text/html; charset=us-ascii > >
Hi,
>
   I'm hoping someone can help me.  I was > wondering what the appropriate starting wage of a grossing tech would > be for a private path lab which works mainly on small skin, gyn, and > oral specimens and processes about 250 cases a day (between 2 > grossers).  Would the pay be any different if the employee had an > M.S.  The position is entry-level.  Thanks very much.  >
 
>
 

> Do you Yahoo!?
> Exclusive Video Premiere - href="http://launch.yahoo.com/video/?1093432&fs=1&redirectURL=http://lau > nch.yahoo.com/promos/britneyspears/">Britney Spears > --0-1595893577-1067722359=:57919-- > > -- __--__-- > > Message: 2 > From: "Gudrun Lang" > To: "Histonetliste" , > "Jen Steinberg" > Date: Sun, 2 Nov 2003 13:19:09 +0100 > Subject: Re: [Histonet] appropriate pay for grossing techs > > This is a multi-part message in MIME format. > > ------=_NextPart_000_001B_01C3A143.E6310980 > Content-Type: text/plain; > charset="iso-8859-1" > Content-Transfer-Encoding: quoted-printable > > Hi Jen, > I dont have an answer for you, but another question. In Austria the = > person, who does crossing, is a pathologist (m.d.).=20 We have several > sorts of tissue f(rom toe to sculp) and I think without = > a medical uni-study, it is very difficult. Even our doctors sometimes = > make mistakes and look over important details. > Is it usual in USA, that histotechs do the crossing? > > best wishes > Gudrun Lang > general hospital Linz, Austria > ----- Original Message -----=20 > From: Jen Steinberg=20 > To: histonet@lists.utsouthwestern.edu=20 > Sent: Saturday, November 01, 2003 10:32 PM > Subject: [Histonet] appropriate pay for grossing techs > > Hi, > I'm hoping someone can help me. I was wondering what the = > appropriate starting wage of a grossing tech would be for a private > path = lab which works mainly on small skin, gyn, and oral specimens > and = processes about 250 cases a day (between 2 grossers). Would the > pay be = > any different if the employee had an M.S. The position is entry-level. > = > Thanks very much. =20 > > ---------------------------------------------------------------------- > -- > -= > ----- > Do you Yahoo!? > Exclusive Video Premiere - Britney Spears > > ------=_NextPart_000_001B_01C3A143.E6310980 > Content-Type: text/html; > charset="iso-8859-1" > Content-Transfer-Encoding: quoted-printable > > > charset=3Diso-8859-1"> > > > > >
Hi Jen,
>
I dont have an answer for you, but = > another=20 > question. In Austria the person, who does crossing, is a pathologist = > (m.d.).=20 >
>
We have several sorts of tissue f(rom = > toe to sculp)=20 > and I think without a medical uni-study, it is very difficult. Even our > = > doctors=20 > sometimes make mistakes and look over important details.
>
Is it usual in USA,  that = > histotechs do=20 > the crossing?
>
 
>
best wishes
>
Gudrun Lang
>
general hospital Linz, = > Austria
> style=3D"PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; = > BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px"> >
----- Original Message -----
> style=3D"BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: = > black">From:=20 > href=3D"mailto:pathologygrl@yahoo.com">Jen=20 > Steinberg >
To: title=3Dhistonet@lists.utsouthwestern.edu=20 > = > href=3D"mailto:histonet@lists.utsouthwestern.edu">histonet@lists.utsouth > w= > estern.edu=20 >
>
Sent: Saturday, November 01, = > 2003 10:32=20 > PM
>
Subject: [Histonet] appropriate > = > pay for=20 > grossing techs
>

>
Hi,
>
   I'm hoping someone can help me.  I was = > wondering what=20 > the appropriate starting wage of a grossing tech would be for a = > private path=20 > lab which works mainly on small skin, gyn, and oral specimens and = > processes=20 > about 250 cases a day (between 2 grossers).  Would the pay be any > = > > different if the employee had an M.S.  The position is = > entry-level. =20 > Thanks very much. 
>
 
>
 
>

>

> Do you Yahoo!?
Exclusive Video Premiere - = > href=3D"http://launch.yahoo.com/video/?1093432&fs=3D1&redirect > UR > L= > =3Dhttp://launch.yahoo.com/promos/britneyspears/">Britney=20 > Spears > > ------=_NextPart_000_001B_01C3A143.E6310980-- > > -- __--__-- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------4B5728D5400BFA6100977CDE Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit My lab does stents as well in Houston! We work with Texas Heart Institute in conjunction with other companies on a regular basis. We do thick saw cut ground and polished sections.
Hope this helps!
If you have any further questions feel free to contact me.
Tiffany:)

Bernard Ian R SSgt 59 CRES/MSROP wrote:

Cathy MAYton in Arizona Does this.

-----Original Message-----
From: Gordon Grant [mailto:grantgd@comcast.net]
Sent: Tuesday, November 04, 2003 5:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Looking for a lab to cut stents

I was wondering if someone out there can give me a recommendation for a
lab which can provide good quality sections of implanted stents.
Specifically, we have been told the FDA likes the thick saw cut, ground
and polished sections rather than microtomed sections.

Thanks Gordon Grant

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-ad min@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Sunday, November 02, 2003 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet digest, Vol 1 #113 - 2 msgs

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Today's Topics:

   1. appropriate pay for grossing techs (Jen Steinberg)
   2. Re: appropriate pay for grossing techs (Gudrun Lang)

-- __--__--

Message: 1
Date: Sat, 1 Nov 2003 13:32:39 -0800 (PST)
From: Jen Steinberg <pathologygrl@yahoo.com>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] appropriate pay for grossing techs

--0-1595893577-1067722359=:57919
Content-Type: text/plain; charset=us-ascii

Hi,
   I'm hoping someone can help me.  I was wondering what the appropriate
starting wage of a grossing tech would be for a private path lab which
works mainly on small skin, gyn, and oral specimens and processes about
250 cases a day (between 2 grossers).  Would the pay be any different if
the employee had an M.S.  The position is entry-level.  Thanks very
much.
 
 

---------------------------------
Do you Yahoo!?
Exclusive Video Premiere - Britney Spears
--0-1595893577-1067722359=:57919
Content-Type: text/html; charset=us-ascii

<DIV>Hi,</DIV>
<DIV>&nbsp;&nbsp; I'm hoping someone can help me.&nbsp; I was wondering
what the appropriate starting wage of a grossing tech would be for a
private path lab which works mainly on small skin, gyn, and oral
specimens and processes about 250 cases a day (between 2
grossers).&nbsp; Would the pay be any different if the employee had an
M.S.&nbsp; The position is entry-level.&nbsp; Thanks very much.&nbsp;
</DIV>
<DIV>&nbsp;</DIV>
<DIV>&nbsp;</DIV><p><hr SIZE=1>
Do you Yahoo!?<br>
Exclusive Video Premiere - <a
href="http://launch.yahoo.com/video/?1093432&fs=1&redirectURL=http:/ /lau
nch.yahoo.com/promos/britneyspears/">Britney Spears</a>
--0-1595893577-1067722359=:57919--

-- __--__--

Message: 2
From: "Gudrun Lang" <gudrun.lang@aon.at>
To: "Histonetliste" <histonet@lists.utsouthwestern.edu>,
        "Jen Steinberg" <pathologygrl@yahoo.com>
Date: Sun, 2 Nov 2003 13:19:09 +0100
Subject: Re: [Histonet] appropriate pay for grossing techs

This is a multi-part message in MIME format.

------=_NextPart_000_001B_01C3A143.E6310980
Content-Type: text/plain;
        charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

Hi Jen,
I dont have an answer for you, but another question. In Austria the =
person, who does crossing, is a pathologist (m.d.).=20
We have several sorts of tissue f(rom toe to sculp) and I think without
=
a medical uni-study, it is very difficult. Even our doctors sometimes =
make mistakes and look over important details.
Is it usual in USA,  that histotechs do the crossing?

best wishes
Gudrun Lang
general hospital Linz, Austria
  ----- Original Message -----=20
  From: Jen Steinberg=20
  To: histonet@lists.utsouthwestern.edu=20
  Sent: Saturday, November 01, 2003 10:32 PM
  Subject: [Histonet] appropriate pay for grossing techs

  Hi,
     I'm hoping someone can help me.  I was wondering what the =
appropriate starting wage of a grossing tech would be for a private path
=
lab which works mainly on small skin, gyn, and oral specimens and =
processes about 250 cases a day (between 2 grossers).  Would the pay be
=
any different if the employee had an M.S.  The position is entry-level.
=
Thanks very much. =20

--------------------------------------------------------------------- ---
-=
-----
  Do you Yahoo!?
  Exclusive Video Premiere - Britney Spears

------=_NextPart_000_001B_01C3A143.E6310980
Content-Type: text/html;
        charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META http-equiv=3DContent-Type content=3D"text/html; =
charset=3Diso-8859-1">
<META content=3D"MSHTML 5.50.4807.2300" name=3DGENERATOR>
<STYLE></STYLE>
</HEAD>
<BODY bgColor=3D#ffffff>
<DIV><FONT face=3DArial size=3D2>Hi Jen,</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>I dont have an answer for you, but =
another=20
question. In Austria the person, who does crossing, is a pathologist =
(m.d.).=20
</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>We have several sorts of tissue f(rom =
toe to sculp)=20
and I think without a medical uni-study, it is very difficult. Even our
=
doctors=20
sometimes make mistakes and look over important details.</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Is it usual in&nbsp;USA, &nbsp;that =
histotechs do=20
the crossing?</FONT></DIV>
<DIV>&nbsp;</DIV>
<DIV><FONT face=3DArial size=3D2>best wishes</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>Gudrun Lang</FONT></DIV>
<DIV><FONT face=3DArial size=3D2>general hospital Linz, =
Austria</FONT></DIV>
<BLOCKQUOTE=20
style=3D"PADDING-RIGHT: 0px; PADDING-LEFT: 5px; MARGIN-LEFT: 5px; =
BORDER-LEFT: #000000 2px solid; MARGIN-RIGHT: 0px">
  <DIV style=3D"FONT: 10pt arial">----- Original Message ----- </DIV>
  <DIV=20
  style=3D"BACKGROUND: #e4e4e4; FONT: 10pt arial; font-color: =
black"><B>From:</B>=20
  <A title=3Dpathologygrl@yahoo.com =
href=3D"mailto:pathologygrl@yahoo.com"> Jen=20
  Steinberg</A> </DIV>
  <DIV style=3D"FONT: 10pt arial"><B>To:</B> <A=20
  title=3Dhistonet@lists.utsouthwestern.edu=20
  =
href=3D"mailto:histonet@lists.ut southwestern.edu">histonet@lists.utsouth
w=
estern.edu</A>=20
  </DIV>
  <DIV style=3D"FONT: 10pt arial"><B>Sent:</B> Saturday, November 01, =
2003 10:32=20
  PM</DIV>
  <DIV style=3D"FONT: 10pt arial"><B>Subject:</B> [Histonet] appropriate
=
pay for=20
  grossing techs</DIV>
  <DIV><BR></DIV>
  <DIV>Hi,</DIV>
  <DIV>&nbsp;&nbsp; I'm hoping someone can help me.&nbsp; I was =
wondering what=20
  the appropriate starting wage of a grossing tech would be for a =
private path=20
  lab which works mainly on small skin, gyn, and oral specimens and =
processes=20
  about 250 cases a day (between 2 grossers).&nbsp; Would the pay be any
=

  different if the employee had an M.S.&nbsp; The position is =
entry-level.&nbsp;=20
  Thanks very much.&nbsp; </DIV>
  <DIV>&nbsp;</DIV>
  <DIV>&nbsp;</DIV>
  <P>
  <HR SIZE=3D1>
  Do you Yahoo!?<BR>Exclusive Video Premiere - <A=20
  =
href=3D" http://launch.yahoo.com/video/?1093432&amp;fs=3D1&amp;redirectUR
L=
=3Dhttp://launch.yahoo.com/promos/britneyspears/">Britney=20
  Spears</A></BLOCKQUOTE></BODY></HTML>

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--------------4B5728D5400BFA6100977CDE-- --------------2B9EB8314574D12854D86640 Content-Type: text/x-vcard; charset=us-ascii; name="Tiffany.L.Sheffield.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Tiffany Sheffield-Lopez Content-Disposition: attachment; filename="Tiffany.L.Sheffield.vcf" begin:vcard n:Sheffield-Lopez;Tiffany tel;fax:713-500-0729 tel;work:713-500-6803 x-mozilla-html:FALSE org:UT Houston Medical School;Orthopaedic Surgery adr:;;6431 Fannin Street, MSB 6.143;Houston ;TX;77030; version:2.1 email;internet:Tiffany.L.Sheffield@uth.tmc.edu title:Supervisor, Bone Histomorphometry & Biomaterials Lab fn:Tiffany Sheffield-Lopez, HT(ASCP) end:vcard --------------2B9EB8314574D12854D86640-- --__--__-- Message: 5 Date: Wed, 05 Nov 2003 16:41:34 +0200 From: Mcleod Reply-To: hmcleod@chempath.uct.ac.za Organization: University of Cape Town To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] dystrophin Thankyou to all who replied to my dystrophin query. Much appreciated. Heather McLeod. --__--__-- Message: 6 From: "Horn, Hazel V" To: 'Diana McCaig' , histonet@lists.utsouthwestern.edu Date: Wed, 5 Nov 2003 08:45:38 -0600 Subject: RE: [Histonet] disposal of tissue Our disposal company requires us to empty the formalin from all the containers. We then collect the formalin and it is also taken away for disposal. I'm wondering what neutralizers anyone is using for formalin so we could dispose of it down the drain?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department. Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital --__--__-- Message: 7 Date: Wed, 5 Nov 2003 07:05:01 -0800 (PST) From: Kelly Booher To: "Horn, Hazel V" Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] disposal of tissue - Formalin Neutralization We neutralize our formalin with Sakura's Tissue-Tek Neutralex. The reaction takes approximately 15 minutes, and then the neutralized formalin is ready to go down the drain. Kelly Booher, HTL (ASCP) Anatomic Pathology Swedish Medical Center, Providence Campus Seattle, WA 98122 --- "Horn, Hazel V" wrote: > Our disposal company requires us to empty the > formalin from all the > containers. We then collect the formalin and it is > also taken away for > disposal. I'm wondering what neutralizers anyone > is using for formalin so > we could dispose of it down the drain?? > > Hazel Horn, HT/HTL (ASCP) > Histology Supervisor > Arkansas Children's Hospital > > Phone - 501.364.4240 > Fax - 501.364.3912 > > -----Original Message----- > From: Diana McCaig [mailto:dmccaig@ckha.on.ca] > Sent: Tuesday, November 04, 2003 2:22 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] disposal of tissue > > > How do labs dispose of pathological waste when > incineration is not available > on site. Do you have to empty each individual 90 ml container or will > companies take them away with the fluid still in > them? I realize most > larger containers are drained and the containers > recycled but was curious to > see what is done with the smaller containers. How > long are the containers > retained and is this process done by lab staff in > the histology department. > Diana McCaig, R.T. > Charge Tech, Histology > Chatham Kent Health Alliance > 519-352-6401 (3347) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information contained in this message may be > privileged and confidential and protected from > disclosure. If the reader of this message is not the > intended recipient, or an employee or agent > responsible for delivering this message to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. > If you have received this communication in error, please notify > us immediately by replying to the message and > deleting it from your computer. Thank you. Arkansas > Children's Hospital > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree --__--__-- Message: 8 Date: Wed, 05 Nov 2003 11:03:37 -0500 From: "Claye Clyatt" To: ,, Subject: RE: [Histonet] disposal of tissue FRC-5 available from S & S Co of GA (912-435-8394). Claye Claye Clyatt Chief Histotechnologist Department of Pathology=20 Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Horn, Hazel V" 11/05/03 09:45AM >>> Our disposal company requires us to empty the formalin from all the containers. We then collect the formalin and it is also taken away for disposal. I'm wondering what neutralizers anyone is using for formalin = so we could dispose of it down the drain?? Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912=20 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca]=20 Sent: Tuesday, November 04, 2003 2:22 PM To: histonet@lists.utsouthwestern.edu=20 Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not = available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was curious = to see what is done with the smaller containers. How long are the containers retained and is this process done by lab staff in the histology department.= Diana McCaig, R.T.=20 Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu=20 http://lists.utsouthwestern.edu/mailman/listinfo/histonet=20 The information contained in this message may be privileged and confidentia= l and protected from disclosure. If the reader of this message is not the = intended recipient, or an employee or agent responsible for delivering = this message to the intended recipient, you are hereby notified that any = dissemination, distribution or copying of this communication is strictly = prohibited. If you have received this communication in error, please = notify us immediately by replying to the message and deleting it from your = computer. Thank you. Arkansas Children's Hospital=20 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu=20 http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 9 Date: Wed, 5 Nov 2003 08:15:40 -0800 From: "Laurie Colbert" To: "Diana McCaig" , "Histonet (E-mail)" Subject: RE: [Histonet] disposal of tissue We have a hazardous waste company that comes out and dumps all of our = specimens for us. He separates the formalin from the specimens in our = Morgue and takes both for disposal. He has all of the proper PPE, = including a respirator. It is expensive, but I feel it is worth every = penny. The time it takes to do the job and the cost of the neutralizer = is expensive, too. If ventilation is not adequate and the formalin = exposure is too high for the employees to perform this procedure safely, = they would have to wear a respirator, and this requires formal = respirator training and fitting. I know there is another company here in southern California that takes = all of the specimens with the formalin still on them. I don't know if = they separate them out later or just burn everything. Laurie Colbert Huntington Hospital Pasadena, CA=20 -----Original Message----- From: Diana McCaig [mailto:dmccaig@ckha.on.ca] Sent: Tuesday, November 04, 2003 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of tissue How do labs dispose of pathological waste when incineration is not = available on site. Do you have to empty each individual 90 ml container or will companies take them away with the fluid still in them? I realize most larger containers are drained and the containers recycled but was = curious to see what is done with the smaller containers. How long are the = containers retained and is this process done by lab staff in the histology = department. Diana McCaig, R.T.=20 Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --__--__-- Message: 10 Date: Wed, 05 Nov 2003 10:33:05 -0600 From: NIDAL E MUVARAK To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Somebody that can do IHC for us??? Hi. I was wondering if there's a lab(s) out there that can do immunos for our lab. We just need help with MMP-2 and MMP-9, I got everything else to work except for these two and I'm getting frustrated. So if anyone knows of a place that provides that, I'd be very grateful if I can get some information on how to get a hold of those people. Thank you for your time. Nidal E Muvarak Associate Research Specialist Vascular Tissue Biomechanics Laboratory Department of Biomedical Engineering University of Wisconsin-Madison 1550 Engineering Dr.; Rm. 2158 Madison, WI 53706-1609 Lab: (608) 265-8921; Office: (608) 265-4205; Home: (608) 256-7934; Cell: (608) 332-6068 http://vtb.bme.wisc.edu --__--__-- Message: 11 Reply-To: "Instrumedics" From: "Instrumedics" To: Date: Wed, 5 Nov 2003 11:50:10 -0500 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs Julian, Do you need an instrument for making the recipient block? If so most labs use the Beecher Arrayer. We market the Paraffin Tape-Transfer system that many labs find very valuable for cutting the section of cores and placing them on a slide. The tape-transfer method eliminates the water bath where cores often are lost. Please visit our web site for details www.instrumedics.com . If you need any further information please contact us. Bernice schiller@instrumedics.com --__--__-- Message: 12 Reply-To: "Instrumedics" From: "Instrumedics" To: Date: Wed, 5 Nov 2003 12:00:03 -0500 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs Eileen, The problem with using the "home" vacuum for cleaning a cryostat is that the debris collected melts and clogs the vacuum bag causing the suctioning to fail. We designed the Cryo-Vac-Away to avoid the clogging problem. The debris that is suctioned as it is generated at the block face is collected in a cold filter that is INSIDE the cryostat. The debris freeze dries and becomes very porous so that the vacuum continues to function effectively. We also have a viral/bacterial filter downstream of the primary filter that will capture pathogens so that the air that exits the system is free of toxins. Bernice schiller@instrumedics.com --__--__-- Message: 13 From: Marion Hiles To: 'eileen dusek' Cc: "Histonet (E-mail)" Date: Wed, 5 Nov 2003 16:56:27 -0000 Subject: RE: [Histonet] couple of questions This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. ------_=_NextPart_001_01C3A3BD.C0D3779A Content-Type: text/plain; charset="iso-8859-1" Answer to question 2: Why would it not be OK to use alcoholic formalin since both, as separate solutions, are used in the majority of tissue processing schedules? We use alcoholic formalin on our VIP. -----Original Message----- From: eileen dusek [mailto:eileen_dusek@yahoo.com] Sent: 04 November 2003 21:39 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] couple of questions Hi Everyone, I have a few questions, 1. Is anyone using a "home" type vacuum for the cryostat. Of course the bags would go in the medical waste. 2. Is Alcoholic formalin OK to put on the VIP tissue processor, there is no acetic acid in the solution. I appreciate all your help. Eileen signature _____ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard ************************************************************************ This e-mail is confidential and privileged. If you are not the intended recipient, please accept our apologies. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents, to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. North Bristol NHS Trust does not guarantee that this material is free from viruses or any other defects, although due care has been taken to minimise the risk. No contracts may be concluded on behalf of North Bristol NHS Trust by means of email communications. Thank you for your cooperation. ************************************************************************ ------_=_NextPart_001_01C3A3BD.C0D3779A Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Answer=20 to question 2: Why would it not be OK to use alcoholic formalin since = both, as=20 separate solutions, are used in the majority of tissue processing = schedules? We=20 use alcoholic formalin on our VIP.
-----Original Message-----
From: eileen dusek=20 [mailto:eileen_dusek@yahoo.com]
Sent: 04 November 2003=20 21:39
To: = histonet@lists.utsouthwestern.edu
Subject:=20 [Histonet] couple of questions

Hi Everyone,
I have a few questions,
1. Is anyone using a "home" type vacuum for the cryostat. Of = course the=20 bags would go in the medical waste.
2. Is Alcoholic formalin OK to put on the VIP tissue processor, = there is=20 no acetic acid in the solution.
 
I appreciate all your help.
 
Eileen


signature

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Protect=20 your identity with Yahoo! Mail = AddressGuard


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This e-mail is confidential and = privileged. If you are not the intended recipient, please accept our = apologies. Please do not disclose, copy or distribute information in = this e-mail or take any action in reliance on its contents, to do so is = strictly prohibited and may be unlawful. Please inform us that this = message has gone astray before deleting it. North Bristol NHS Trust = does not guarantee that this material is free from viruses or any other = defects, although due care has been taken to minimise the risk. No = contracts may be concluded on behalf of North Bristol NHS Trust by = means of email communications.

Thank you for your cooperation. =

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------_=_NextPart_001_01C3A3BD.C0D3779A-- --__--__-- Message: 14 From: "Thom Jensen" To: histonet@lists.utsouthwestern.edu Date: Wed, 05 Nov 2003 17:26:35 +0000 Subject: [Histonet] Re: Histonet digest, Vol 1 #116 - 24 msgs
In regard to Instrumedics comments, The reciepent block is the blank paraffin block used as the array block for inserting your cores into.
 
I do not recommend using the Tape-Transfer System for paraffin sectioning.  I know of no one who has had success using it for paraffin.  The sections are compressed and often unreadable.
 
Thom Jensen
 
For more information on Tissue Microarray instruction visit:  www.arrayworkshop.com
 
 
 

Is your computer infected with a virus? Find out with a FREE computer virus scan from McAfee. Take the FreeScan now! --__--__-- Message: 15 Date: Wed, 5 Nov 2003 12:46:12 -0500 (EST) From: Ramesh Subrahmanyam To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Distinguishing monocytes from granulocytes Hello Histonetters, I am trying to distinguish immature monocytes from immature granulocytes in Balb/c mice. Can anyone suggest antibodies for FACS or immunohistochemistry or RT-PCR markers that would help in this? Suggestions on other methods would be welcome too. Thanks in advance, Ramesh Brandeis University Massachusetts --__--__-- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest From Jackie.O'Connor <@t> abbott.com Tue Nov 11 06:32:26 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Picric Acid Message-ID: I've always been instructed by HAZMAT officials that old picric acid (particularly if crystals have formed under the lid) is an acute explosion hazard. Just opening the jar can cause a large explosion. I've been in two situations (once in Memphis, Tennessee, once on Honolulu, Hawaii) when either the bomb squad or Army Explosive Ordinance Division came out to remove our picric acid. (Not at my insistance, but theirs. I would not open a jar of dry picric acid with crystal formation. Go to this website for more information. http://www-ehs.ucsd.edu/lab/2009.htm#c Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 John Kiernan Sent by: histonet-admin@lists.utsouthwestern.edu 11/10/2003 11:11 PM Please respond to jkiernan To: jim , Histonet Listserver cc: Subject: Re: [Histonet] Picric Acid Wash under a tap. Put some water in the jar if it has dried out. Picric acid explodes if its temperature goes above 300C. Sources: Lange's Handbook of Chemistry, just checked; also remembered from various textbooks read over the years. This cannot happen in the presence of liquid water. Nearly all anecdotes about picric acid explosions in labs are urban legends (= untrue). For confirmation of this assertion, check it out with Google, but allow a couple of hours and use your brain to evaluate the produce. __________________________________ > jim wrote: > > Has anyone information or data on the safe disposal > of Picric Acid (crystals have formed on lid of > bottle). > > Thanks > > Jim Manavis > Senior Hospital Scientist > Department of Neuropathology > Institute of Medical & Veterinary Science > Adelaide, SA, 5000 > Australia > Phone: 61-08-8222-3668 > FAX: 61-08-8222 3204 > e.mail: jim.manavis@imvs.sa.gov.au > Disclaimer: Not this little black duck! > -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031111/18e7dc34/attachment.htm From Marion.Hiles <@t> north-bristol.swest.nhs.uk Tue Nov 11 07:54:06 2003 From: Marion.Hiles <@t> north-bristol.swest.nhs.uk (Marion Hiles) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Amyloid Precursor Protein Message-ID: <2EE924DF60902943AC6E2EF35155451F19CCE8@nbfexch03.north-bristol.nhs> We use ZYMED BAPP Cat No. 13-0200 at 1:3000 overnight after microwaving in citrate buffer pH6. Bob Quilty Dept of Neuropathology Frenchay Hospital Bristol UK -----Original Message----- From: Dana Settembre [mailto:settembr@umdnj.edu] Sent: 10 November 2003 16:39 To: histonet@pathology.swmed.edu Subject: [Histonet] Amyloid Precursor Protein Has anyone worked up Amyloid Precursor Protein (APP) on FFPE human tissue? I have NovoCastra's monoclonal antibody. I have used Alzheimer's disease brain tissue that works with other antibodies. I use a very good Target Retreival solution from DakoCytomation in a steamer for 40 minutes. I have used it at the recommended dilutions and I have used them more potent than that too. Our neuropathologist tells me that it has been in use regularly for some time now. HELP! Dana Settembre Immunohistochemistry Lab Unversity Hospital-UMDNJ Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ This e-mail is confidential and privileged. If you are not the intended recipient, please accept our apologies. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents, to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. North Bristol NHS Trust does not guarantee that this material is free from viruses or any other defects, although due care has been taken to minimise the risk. No contracts may be concluded on behalf of North Bristol NHS Trust by means of email communications. Thank you for your cooperation. ************************************************************************ From d.a.faichney <@t> stir.ac.uk Tue Nov 11 08:02:26 2003 From: d.a.faichney <@t> stir.ac.uk (Deborah Faichney) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Torn tissues followup Message-ID: <570E2BEE7BC5A34684EE5914FCFC368C190D57@fillan.stir.ac.uk> Hello Julien, Which species of fish are you working with? -We routinely work with a variety of fish and find different techniques are required to obtain good sections dependent upon the species, size etc. When fixing the fish are you opening the abdomen & making an incision into the head to allow the penetration of fixative? -Poorly fixed skin /muscle causes 'holes' and 'tearing' in sections. We cut, after surface decalcification for approx. 30 min-1hr , at 5 microns and float out on to distilled water in a heated waterbath. The slide is then put into an oven set a couple of degrees above the melting point of the wax for 1hr prior to staining with H&E. When you say, ''But this methods complicates recognition of sections to the fish length.......enables us to keep track of how far we are in the fishe's body'' -If you need to be able to match serial/consecutive sections to the block containing one fish you could give each whole fish a unique number/code and also write the section number to the slide that way you shouldn't have any problem with identification from original blocks eg. fish 1 could be 1-1, 1-2, 1-3 etc fish 2 could be 2-1, 2-2, 2-3 etc Just a few thoughts although it is difficult to help out with your problems without knowing more information. if you are interested you could e-mail me directly and I will send you a copy of our methods. Hopefully this helps you. Debbie Debbie Faichney Institute of Aquaculture University of Stirling Stirling Scotland FK9 4LA -----Original Message----- From: Julien Lambrey de Souza [mailto:jlambrey@hotmail.com] Sent: 10 November 2003 14:04 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Torn tissues followup Hello to all histonetters, I am having problems with torn tissues and I have already posted a message asking what may be the causes. Thanks for the various replies. Most of you aksed me more details about the procedure in order to try and define a problem. so here goes: Small fish (5cm) where fixed in formalin and then preserved in 70% EtOh Processing is done on an automated processor 2 hours in 95%alcohol 2 hours in 100% 3 hours in xylene 1 hour in wax (paraplus) 1 hour in wax with vacuum. Embedding is done with paraplus wax on a histocenter II. Sectioning is done at 7 microns, transversally to body length. To mount secions on slides, 2 methods are used. The first is a heated waterbath to unrinkle the tape. But this methods complicates recognition of sections to the fish length. That's why we prefer to lay the tapes on a black cardboard paper, put some water on slides, put the tape on the slide and then heat slightly the slide on a slide warmer so the tape expands to unrinkle on the buble of water. This technique has worked fine up to now and enables us to keep track of how far we are in the fishe's body. Staining is done on an automated stainer Xylene 3 min (x3) 100% alcohol 2 min 100% 3 min 95% 2 min (x2) Water 2 min Hematoxylin 4 min Water 2 min Bluing reagent 2 min Water 3 min 95% 30 sec Eosine 2 min 95% 1 min (x2) 100% 1 min 100% 2 min Xylene 3 min (x3) Coverslips are mounted automatically. So there it is. I don't think the tearing is due to microtome blade since I use a new disposable blade each time I have a doubt. So, according to the comments we received up to now, the parameters I'm going to change are the processing times in alcohol and xylene. I am going to lower them but raise the time in parafin. I have to check the parafin temperature as I was told an excessively hot wax will not penetrate as well. Also I'm going to try and reduce slide warming temperature but leave them dry longer, since wet sections may slide off during staining. So, if you have any further suggestion to help me solve my problem, all comments are welcome. Chears, Julien De souza. _________________________________________________________________ Add photos to your messages with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin. msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is a university established in Scotland by charter at Stirling, FK9 4LA. Privileged/Confidential Information may be contained in this message. If you are not the addressee indicated in this message (or responsible for delivery of the message to such person), you may not disclose, copy or deliver this message to anyone and any action taken or omitted to be taken in reliance on it, is prohibited and may be unlawful. In such case, you should destroy this message and kindly notify the sender by reply email. Please advise immediately if you or your employer do not consent to Internet email for messages of this kind. From hmcleod <@t> chempath.uct.ac.za Tue Nov 11 08:02:50 2003 From: hmcleod <@t> chempath.uct.ac.za (Mcleod) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] CD21 Message-ID: <3FB0EC0A.F47AA75D@chempath.uct.ac.za> Dear All We use the Dakocytomation (M0784) CD21 with 10mins Protease AR on the Ventana Nexes - IVIEW DAB. We get epthelial staining as well as the expected pattern of staining. The spec sheet states that this does occur. The problem is our path's are not happy. Is anybody using HIER. If yes what dilutions are used? Thanks for all answers. Heather From sa.drew <@t> hosp.wisc.edu Tue Nov 11 08:18:38 2003 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] RE: CD21 Message-ID: We do not use HIER, we use Ventana's protease 1 for 8" with BioCare Medical's CD21...have you tried reducing the protease time or diluting the antibody more? Maybe a different clone would please them more... Sally Ann Drew-MT(ASCP) Univ. of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. VAH-DM223 Madison, WI 53792-2472 608-265-6596 sa.drew@hosp.wisc.edu -----Original Message----- From: Mcleod [mailto:hmcleod@chempath.uct.ac.za] Sent: Tuesday, November 11, 2003 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD21 Dear All We use the Dakocytomation (M0784) CD21 with 10mins Protease AR on the Ventana Nexes - IVIEW DAB. We get epthelial staining as well as the expected pattern of staining. The spec sheet states that this does occur. The problem is our path's are not happy. Is anybody using HIER. If yes what dilutions are used? Thanks for all answers. Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Myri37 <@t> aol.com Tue Nov 11 08:28:21 2003 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] need help for epon Message-ID: <134.27953bea.2ce24c05@aol.com> hello i have embedded thin tissue in epon, after postfixation with osmium i stuck sections of 5 um on slides with haupt's solution, deplastized in ethoxide of sodium in heat for 30 minutes and after that stained Goldner's trichrome tissue was not coloured, and i dont understand why, is it because of osmium ? pleaze do you have a protocol of Goldner's trichrome especially for epon not for parrafin sections, or another stain to show collagen and mineralized tissu ? Myriam Natural implant France -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031111/c45bde09/attachment.htm From carl.hobbs <@t> kcl.ac.uk Tue Nov 11 08:45:33 2003 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] anti GFP Ab reagent Message-ID: <00c401c3a862$762e8f10$e8345c9f@Carlos> If you're using formalin-fixed, pwax sections. molecular probes A6455 has given me excellent results when detected with std streptABC px DAB method. Ag retrieval reqd using TRIS 1.23g/L pH10 Carl -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031111/b536dbcd/attachment.htm From Ronnie_Houston <@t> bshsi.com Tue Nov 11 09:00:32 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] e-cadherin antibody Message-ID: <530361BF03351B4CAE5270A05D3037B5FE0512@bsrexms01.BSHSIR.COM> Which clone are people using for e-caherin IHC on FFPE tissues? Thanks Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From sa.drew <@t> hosp.wisc.edu Tue Nov 11 09:09:02 2003 From: sa.drew <@t> hosp.wisc.edu (Drew Sally A.) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] e-cadherin antibody Message-ID: ECH-6 Sally Ann Drew-MT(ASCP) Univ. of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. VAH-DM223 Madison, WI 53792-2472 608-265-6596 sa.drew@hosp.wisc.edu -----Original Message----- From: Houston, Ronnie [mailto:Ronnie_Houston@bshsi.com] Sent: Tuesday, November 11, 2003 9:01 AM To: Histonet (E-mail) Subject: [Histonet] e-cadherin antibody Which clone are people using for e-caherin IHC on FFPE tissues? Thanks Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Tue Nov 11 09:16:59 2003 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] e-cadherin antibody Message-ID: 4A2 C7 from Zymed, order #18-0223 > -----Original Message----- > From: Houston, Ronnie [SMTP:Ronnie_Houston@bshsi.com] > Sent: Tuesday, November 11, 2003 9:01 AM > To: Histonet (E-mail) > Subject: [Histonet] e-cadherin antibody > > > Which clone are people using for e-caherin IHC on FFPE tissues? > > Thanks > Ronnie > > Ronnie Houston > Regional Histology Operations Manager > Bon Secours HealthPartners Laboratories > 5801 Bremo Road > Richmond, VA 23226 > (804) 287 7972 > ronnie_houston@bshsi.com > > __________________________________________________________________________ > ______________________________________________________ > __________________________________________________________________________ > ______________________________________________________ > > The information in this communication is intended to be confidential to > the Individual(s) and/or Entity to whom it is addressed. > It may contain information of a Privileged and/or Confidential nature, > which is subject to Federal and/or State privacy regulations. > In the event that you are not the intended recipient or the agent of the > intended recipient, do not copy or use the information > contained within this communication, or allow it to be read, copied or > utilized in any manner, by any other person(s). Should > this communication be received in error, please notify the sender > immediately either by response e-mail or by phone at 410-442-3250, > and permanently delete the original e-mail, attachment(s), and any copies. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From juan.gutierrez <@t> christushealth.org Tue Nov 11 09:27:59 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] e-cadherin antibody Message-ID: Zymed's 4A2C7. -----Original Message----- From: Houston, Ronnie [mailto:Ronnie_Houston@bshsi.com] Sent: Tue 11/11/2003 9:00 AM To: Histonet (E-mail) Cc: Subject: [Histonet] e-cadherin antibody Which clone are people using for e-caherin IHC on FFPE tissues? Thanks Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.j.savage <@t> gsk.com Tue Nov 11 09:29:47 2003 From: tony.j.savage <@t> gsk.com (tony.j.savage@gsk.com) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #125 - 17 msgs Message-ID: Your mouse spleen should provide you with the perfect internal control for Perls stain. Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm From juan.gutierrez <@t> christushealth.org Tue Nov 11 09:33:38 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] RE: CD21 Message-ID: Pardon the interruption, but what dilution are you using on the Benchmark? Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare -----Original Message----- From: Drew Sally A. [mailto:sa.drew@hosp.wisc.edu] Sent: Tue 11/11/2003 8:18 AM To: Histonet Cc: Subject: [Histonet] RE: CD21 We do not use HIER, we use Ventana's protease 1 for 8" with BioCare Medical's CD21...have you tried reducing the protease time or diluting the antibody more? Maybe a different clone would please them more... Sally Ann Drew-MT(ASCP) Univ. of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. VAH-DM223 Madison, WI 53792-2472 608-265-6596 sa.drew@hosp.wisc.edu -----Original Message----- From: Mcleod [mailto:hmcleod@chempath.uct.ac.za] Sent: Tuesday, November 11, 2003 8:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD21 Dear All We use the Dakocytomation (M0784) CD21 with 10mins Protease AR on the Ventana Nexes - IVIEW DAB. We get epthelial staining as well as the expected pattern of staining. The spec sheet states that this does occur. The problem is our path's are not happy. Is anybody using HIER. If yes what dilutions are used? Thanks for all answers. Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakerj <@t> umich.edu Tue Nov 11 10:09:23 2003 From: bakerj <@t> umich.edu (John Baker) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] thin sectioning of PMMA Message-ID: Howdy all, Does anyone have a good protocol for getting thin (4-5 micron) sections from PMMA embedded bone? We use a Riechert-Jung Polycut with tungsten knives for sectioning. A problem in the past has been getting the sections to stick to a slide with curling and wrinkles being the cause. Fluorescence of the sections will be looked at in future studies. Thanks for any assistance in this matter! John -- ------------------------------------------------------- Today is the Tomorrow you worried about Yesterday. Was it worth it? Birthdays are good for you. Statistics show that the people who have the most live the longest. ------------------------------------------------------- John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 400 North Ingalls, G160 Ann Arbor, MI 48109-0486 Main lab office phone:734-763-9674 Histology office:734-936-1635 From t-kuzniar <@t> md.northwestern.edu Tue Nov 11 10:07:48 2003 From: t-kuzniar <@t> md.northwestern.edu (t-kuzniar@md.northwestern.edu) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies Message-ID: <200311111608.hABG843j014587@casbah.it.northwestern.edu> Has anyone had any experience with polyclonal antibody against b-galactosidase? What reporter system have you used? Thank you. Tom Kuzniar From tbourm <@t> olympicmedical.org Tue Nov 11 11:00:29 2003 From: tbourm <@t> olympicmedical.org (Tasha Bourm) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org From RBARNHART <@t> summithealth.org Tue Nov 11 11:28:35 2003 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbourm <@t> olympicmedical.org Tue Nov 11 11:38:47 2003 From: tbourm <@t> olympicmedical.org (Tasha Bourm) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: We are looking for a outside source for helicobacter QC because we do not get many positive cases. -----Original Message----- From: Tasha Bourm [mailto:tbourm@olympicmedical.org] Sent: Tuesday, November 11, 2003 9:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HELICOBACTER CONTROLS Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Nov 11 12:18:02 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: I don't understand why labs can't get positive tissue for control purposes from their own lab. Do you not see many gastric biopsies? Richard Cartun >>> "Rebecca Barnhart" 11/11/03 12:28PM >>> We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From housen1 <@t> yahoo.com Tue Nov 11 12:24:15 2003 From: housen1 <@t> yahoo.com (Nancy House) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] (no subject) Message-ID: <20031111182415.15887.qmail@web11208.mail.yahoo.com> I am a dermatopathologist who will be starting up a small lab (5000spec per yr) and I have no experience with histology equipment. Any suggestions for processors and stainers (or any other advice anyone would like to share!) would be greatly appreciated. I will not have outside ventilation and I think my volume will only increase slightly in the near future. Thank you, Nancy House, MD Valley Dermatopathology Sewickely, PA __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From tmhpath <@t> amigo.net Tue Nov 11 12:46:35 2003 From: tmhpath <@t> amigo.net (Michelle D. Moore) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Helicobacter controls Message-ID: <005901c3a884$238257c0$4000a8c0@ever> I use an outside source for my helicobacter controls. If you get the American Master Tech Scientific catalog you can order them directly. I have been using them for almost a year and a half and have had great results. If you don't have the catalog the phone number is 800-860-4073, they are located in Lodi, CA. If you need any other information let me know. Best of luck. Michelle Moore The Memorial Hospital Craig, CO -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031111/f579b15a/attachment.htm From Sue.Kapoor <@t> uhsi.org Tue Nov 11 13:14:12 2003 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5500@khmcexch.uhsi.org> We see gastric biopsies quite often and do use them for controls, but I hate to use up all of the patient tissue...just in case it's needed in the future. Sue Kapoor HT (ASCP) Histology Supervisor Kenosha Medical Center Kenosha, WI -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, November 11, 2003 12:18 PM To: histonet@lists.utsouthwestern.edu; RBARNHART@summithealth.org Subject: Re: [Histonet] HELICOBACTER CONTROLS I don't understand why labs can't get positive tissue for control purposes from their own lab. Do you not see many gastric biopsies? Richard Cartun >>> "Rebecca Barnhart" 11/11/03 12:28PM >>> We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Tue Nov 11 13:28:07 2003 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: We do get gastric biopsies but very few are positive (like 2 in the past year). The tissue is so small and it doesn't take long to cut through it (plus we like to keep patient tissue just incase it is needed in the future). That is why we grew our own and planted it on a colon. We can have controls anytime we want that can be cut away. Becky >>> "Richard Cartun" 11/11 1:18 PM >>> I don't understand why labs can't get positive tissue for control purposes from their own lab. Do you not see many gastric biopsies? Richard Cartun >>> "Rebecca Barnhart" 11/11/03 12:28PM >>> We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nick.kirk3 <@t> btopenworld.com Tue Nov 11 13:58:44 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS In-Reply-To: Message-ID: Um, surely the whole point of a Helicobacter control, is that it has Helicobacter in it, not E. coli? Helicobacter and E. coli look very different for one thing, so by having a control with E. coli in it wouldn't give you the necessary reference point as it were, to the appearance of Helicobacter positivity. An inexperienced person might miss a positive if they don't see the real thing. If you're only interested in demonstrating that there's bacteria present, why not use the same control that you use for a gram stain? Personally I would reject any stained slide done in my lab for Helicobacter, that wasn't accompanied by a Helicobacter positive control. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Rebecca Barnhart Sent: 11 November 2003 17:29 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELICOBACTER CONTROLS We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Nov 11 14:15:41 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] (no subject) Message-ID: Stay away from Leica. -----Original Message----- From: Nancy House [mailto:housen1@yahoo.com] Sent: Tue 11/11/2003 12:24 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] (no subject) I am a dermatopathologist who will be starting up a small lab (5000spec per yr) and I have no experience with histology equipment. Any suggestions for processors and stainers (or any other advice anyone would like to share!) would be greatly appreciated. I will not have outside ventilation and I think my volume will only increase slightly in the near future. Thank you, Nancy House, MD Valley Dermatopathology Sewickely, PA __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From georgecole <@t> ev1.net Tue Nov 11 14:15:52 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] To All Those Who Received Muscle/Nerve packets Message-ID: <000001c3a890$9c4e9ae0$054dbad0@hppav> Mauricio Morales spotted a problem in the first DVD of muscle/nerve techniques. It has to do with the freezing of the muscle. I show a thin walled plastic mold with just a little OCT in the bottom of it to hold the muscle or nerve in the mold during freezing. The next shot shows a mold with muscle in it more completely covered with frozen OCT. Mauricio points out that this is confusing. Where did that added OCT come from? ----sorry for the confusion. The second shot, with the more OCT around the tissue, was taped a year or so after the actual freezing episode. The idea of that second shot was just to show how to bolster the tissue with OCT, to make a good block for cutting. The block I used for that shot did have more OCT frozen around it than it would have had if it had just been frozen. Sorry about that. The actual sequence is this: 1. A small amount of OCT is put in the bottom of a thin walled mold to keep the tissue in the mold while it is frozen. The tissue is properly oriented for cutting cross sections. It is frozen. 2: The layer of frozen OCT holding the tissue in the mold is thin and quite fragile, so a little OCT is added to the mold in the cryostat and quickly frozen---just enough to make the muscle/frozen OCT strong enough to survive removal from the mold. 3: The tissue is removed from the mold and placed on a freezing station in the cryostat. An object holder, kept at room temperature, is put into the cryostat on a freezing station. A blob of OCT is quickly put on the object holder and the tissue in the frozen OCT/muscle block is stood up in the OCT---oriented to stand up so that cross sections can be cut. The OCT will quickly freeze holding the block in its cutting position. 4. The standing block now needs to be bolstered with OCT for cutting. OCT is added in discrete steps and quickly frozen, building up by degrees a rectangle of frozen OCT. The object holder with the block on it is then put in the cutting position. It is trimmed to make all edges of the rectangle surrounding the tissue straight and clean and the corners square. The block is positioned in the cryostat to make the long edge of the block parallel with the knife edge. Sections are cut at 8 microns to nearly fill the slide, and one section, 10 microns thick, completes the filling of the slide. Thanks, Mauricio. From asmith <@t> mail.barry.edu Tue Nov 11 14:28:43 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Reality check: Part 2 Message-ID: <494304423C63E246A5CF87A3AEEB577011ED97@bumail01.barrynet.barry.edu> "The price that Americans pay for their exceptional freedom is an extremely high prevalence of fraud." - Domingo Sarmiento. -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, November 07, 2003 7:20 AM To: Histonet Subject: [Histonet] Reality check: Part 2 Hi, it's me again. What do you think about a company that promotes a better product that you test out, then decide to purchase that product, only to find out that the product doesn't work? I was given these ink pens that were supposed to be better than the Securline Markers. These pens were supposed to be alcohol and xylene resistant. The sample product worked great, so I purchased 100 of these pens. They worked for a few days, then bam, 25 cassettes lost all their numbers. Of course all these were GI cases. We were barely able to see what number was written on the cassettes. Gratefully, after many hours of matching the blocks to the gross descriptions, we were able to make new cassettes and match them up to the correct cases. So, as only I can do, I packaged up the cassettes that had the numbers washed off, put all these pens in a box with a letter explaining what happened and mailed them off to the company. That was a month ago. Now I know NSH was coming up so I let things go. Now, after one month and after the NSH, I still haven't heard back from this company. Is it me or are companies putting out inferior products these days? I know I sound like a whiner, but come on. What happened to taking responsibilities for your actions. I'm really not that difficult to get along with. I just want decent, dependable products. Am I holding these vendors at too much of a high standard? What ever happened to credibility? Joe Nocito BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From philip.bergin <@t> microbio.gu.se Tue Nov 11 14:28:50 2003 From: philip.bergin <@t> microbio.gu.se (Phil Bergin) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS In-Reply-To: Message-ID: <000301c3a892$6aeacc10$8a6c73d5@mb5> Why not grow Helicobacter pylori instead and smear that? ----------------------------------------------------------------- Philip Bergin G?teborg University Department of Medical Microbiology and Immunology Box 435, SE-405 30 G?teborg, Sweden Phone: +46-31-7736213 Fax: +46-31-7736205 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: den 11 november 2003 18:29 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELICOBACTER CONTROLS We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Tue Nov 11 15:13:35 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] heart IHC work Message-ID: Friends, I am involved in a project that requires doing IHC for Lamin A/C on heart tissue. If anyone has experience with this please tell me all you know. Also, since most of this is DNA work they have pieces of fresh heart frozen at -70 dC. What would be the best way to fix and process the frozen heart so that it would be well preserved for IHC. Thank you for advise in this matter Patsy From peptolab <@t> hamptons.com Tue Nov 11 15:14:41 2003 From: peptolab <@t> hamptons.com (peptolab) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Picric BOOM Message-ID: <001d01c3a898$d4849560$dca5bd18@JEFF> Call the bomb squad. Seriously it is explosive and you need to contact Haz Mat people about wetting it down and removing it. Jeff Silverman From tbarnhart <@t> innernet.net Tue Nov 11 15:59:46 2003 From: tbarnhart <@t> innernet.net (Becky Barnhart) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS References: <000301c3a892$6aeacc10$8a6c73d5@mb5> Message-ID: <00aa01c3a89f$1fc50d60$0000a398@Tuckey> We already grow e coli for microbiology. ----- Original Message ----- From: "Phil Bergin" To: "'Rebecca Barnhart'" ; Sent: Tuesday, November 11, 2003 3:28 PM Subject: RE: [Histonet] HELICOBACTER CONTROLS Why not grow Helicobacter pylori instead and smear that? ----------------------------------------------------------------- Philip Bergin G?teborg University Department of Medical Microbiology and Immunology Box 435, SE-405 30 G?teborg, Sweden Phone: +46-31-7736213 Fax: +46-31-7736205 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: den 11 november 2003 18:29 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELICOBACTER CONTROLS We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Nov 11 17:00:58 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Picric Acid Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E09F@simba.kids> John and Jim, A recent newspaper article in Sydney may be appropriate here. The gist of it is as follows: ACID EVACUATION About 160 people were evacuated from a factory and homes in Sydney yesterdat afternoon after authorities identified a dangerous chemical. NSW Fire Brigade spokesman Ian Krimmer said a small amount of an acid, which had the potential to react violently, had been found in a jar and removed from a factory in Kingsgrove. "A small amount of picric acid in a jar [was] in an unstable condition." Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Tuesday, 11 November 2003 4:12 PM To: jim; Histonet Listserver Subject: Re: [Histonet] Picric Acid Wash under a tap. Put some water in the jar if it has dried out. Picric acid explodes if its temperature goes above 300C. Sources: Lange's Handbook of Chemistry, just checked; also remembered from various textbooks read over the years. This cannot happen in the presence of liquid water. Nearly all anecdotes about picric acid explosions in labs are urban legends (= untrue). For confirmation of this assertion, check it out with Google, but allow a couple of hours and use your brain to evaluate the produce. __________________________________ > jim wrote: > > Has anyone information or data on the safe disposal > of Picric Acid (crystals have formed on lid of > bottle). > > Thanks > > Jim Manavis > Senior Hospital Scientist > Department of Neuropathology > Institute of Medical & Veterinary Science > Adelaide, SA, 5000 > Australia > Phone: 61-08-8222-3668 > FAX: 61-08-8222 3204 > e.mail: jim.manavis@imvs.sa.gov.au > Disclaimer: Not this little black duck! > -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JGordon <@t> cellmarque.com Tue Nov 11 17:09:53 2003 From: JGordon <@t> cellmarque.com (Jeff Gordon) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: Cell Marque supplies IHC tested Helicobacter pylori positive controls, catalog number CMS433. Jeff Gordon Cell Marque Corp. 1-800-665-7284 -----Original Message----- From: Tasha Bourm [mailto:tbourm@olympicmedical.org] Sent: Tuesday, November 11, 2003 11:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HELICOBACTER CONTROLS Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From L.Driessen <@t> orthop.umcn.nl Wed Nov 12 00:37:46 2003 From: L.Driessen <@t> orthop.umcn.nl (Driessen, L.) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Re: thin sectioning of PMMA Message-ID: <5B31B9F59E138A409E8F7C2183FF36F00DF492@umcnet13.umcn.nl> Hi, Maybe this can help. I always use gelatine-coated glasscarri?rs and for stretching out the slices I use a mixture consisting of 7 parts ethanol and 3 parts 2-butyloxy-ethanol. After stretching (done with help of smal brushes and forceps) I cover the slices with a plastic foil (cut out of a sandwich-bag), rub out the excess of stretchingmedium with a tissuepaper and place a glass-carri?r on top (you can make a stack if you want). For drying I clamp the stack between two wooden blocks with the means of a glueing clamp and let it dry for at least one night at 37?C. Leon Driessen, Orthopaedic Research Laboratory, UMCN-St. Radboud, Nijmegen, The Netherlands. From alexander.nader <@t> wgkk.sozvers.at Wed Nov 12 01:07:01 2003 From: alexander.nader <@t> wgkk.sozvers.at (Nader, Alexander) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] e-cadherin antibody Message-ID: <4FE843CBBAC1D211A8150008C70952DAAE7B2D@hk01nt05.hkh.wgkk.sozvers.at> > Which clone are people using for e-caherin IHC on FFPE tissues? > Novocastra E-Cadherin clone 36B5 (IgG1) 1:50. Alexander Nader Inst. f. Pathology, Hanusch-Hospital Vienna, Austria From alexander.nader <@t> wgkk.sozvers.at Wed Nov 12 01:08:41 2003 From: alexander.nader <@t> wgkk.sozvers.at (Nader, Alexander) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] pax-5 Message-ID: <4FE843CBBAC1D211A8150008C70952DAAE7B2E@hk01nt05.hkh.wgkk.sozvers.at> Does anybody use pax-5 on FFPE tissue? Which clone? Which dilution? Alexander Nader Inst. f. Pathology, Hanusch-Hospital Vienna, Austria From RBARNHART <@t> summithealth.org Wed Nov 12 07:09:25 2003 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS Message-ID: The reason we are using e coli for a helicobacter positive control is just to show the stain is working. If it is positive for e coli it will be positive for helicobacter. Our pathologist does know the difference between helicobacter and e coli, he is the one that has decided to get a positive control this way. We do not do IHC stains, just a geimsa stain. >>> "Nick Kirk" 11/11 2:58 PM >>> Um, surely the whole point of a Helicobacter control, is that it has Helicobacter in it, not E. coli? Helicobacter and E. coli look very different for one thing, so by having a control with E. coli in it wouldn't give you the necessary reference point as it were, to the appearance of Helicobacter positivity. An inexperienced person might miss a positive if they don't see the real thing. If you're only interested in demonstrating that there's bacteria present, why not use the same control that you use for a gram stain? Personally I would reject any stained slide done in my lab for Helicobacter, that wasn't accompanied by a Helicobacter positive control. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Rebecca Barnhart Sent: 11 November 2003 17:29 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELICOBACTER CONTROLS We were having trouble finding a positive control also. So we had our micro department grow e-coli. We then smeared it on a piece of colon, processed, embedded, cut, stained and picked the best section. Becky >>> Tasha Bourm 11/11 12:00 PM >>> Does anyone have a good source for helicobacter QC cut slides or blocks? Tasha R. Bourm Olympic Medical Center Histology Department 939 Caroline Street Port Angeles, WA 98362 tbourm@olympicmedical.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Wed Nov 12 07:27:48 2003 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] testing for plain text only Message-ID: <004501c3a920$c3b19a70$e8345c9f@Carlos> From Rcartun <@t> harthosp.org Wed Nov 12 08:39:03 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] pax-5 Message-ID: We use PAX-5 from BD Transduction Laboratories (www.bdsciences.com); 1:50 for 30' or 1:200 overnight following HIER in a 96 degree C. waterbath for 40'. Richard Cartun >>> "Nader, Alexander" 11/12/03 02:08AM >>> Does anybody use pax-5 on FFPE tissue? Which clone? Which dilution? Alexander Nader Inst. f. Pathology, Hanusch-Hospital Vienna, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Nov 12 08:42:37 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] HELICOBACTER CONTROLS In-Reply-To: References: Message-ID: We use colon controls for the same reason. There is always some fecal matter on the surface and bacteria are numerous. Colon is very easy to get. Bill At 8:09 AM -0500 11/12/03, Rebecca Barnhart wrote: >The reason we are using e coli for a helicobacter positive control is >just to show the stain is working. If it is positive for e coli it will >be positive for helicobacter. Our pathologist does know the difference >between helicobacter and e coli, he is the one that has decided to get a >positive control this way. We do not do IHC stains, just a geimsa >stain. -- ______________ Bill Blank, MD Heartland Lab, Inc From AFeatherstone <@t> KaleidaHealth.Org Wed Nov 12 08:40:05 2003 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Myoadenylate Deaminase Muscle Stain Message-ID: We are having trouble finding AMP(adenosine monophosphate). we used to get it from sigma catalog A-1877. They no longer deal with this product. Does anyone know where we can order this? Annette Featherstone HT/MLT -----Original Message----- From: George Cole [mailto:georgecole@ev1.net] Sent: Tuesday, November 11, 2003 15:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] To All Those Who Received Muscle/Nerve packets Mauricio Morales spotted a problem in the first DVD of muscle/nerve techniques. It has to do with the freezing of the muscle. I show a thin walled plastic mold with just a little OCT in the bottom of it to hold the muscle or nerve in the mold during freezing. The next shot shows a mold with muscle in it more completely covered with frozen OCT. Mauricio points out that this is confusing. Where did that added OCT come from? ----sorry for the confusion. The second shot, with the more OCT around the tissue, was taped a year or so after the actual freezing episode. The idea of that second shot was just to show how to bolster the tissue with OCT, to make a good block for cutting. The block I used for that shot did have more OCT frozen around it than it would have had if it had just been frozen. Sorry about that. The actual sequence is this: 1. A small amount of OCT is put in the bottom of a thin walled mold to keep the tissue in the mold while it is frozen. The tissue is properly oriented for cutting cross sections. It is frozen. 2: The layer of frozen OCT holding the tissue in the mold is thin and quite fragile, so a little OCT is added to the mold in the cryostat and quickly frozen---just enough to make the muscle/frozen OCT strong enough to survive removal from the mold. 3: The tissue is removed from the mold and placed on a freezing station in the cryostat. An object holder, kept at room temperature, is put into the cryostat on a freezing station. A blob of OCT is quickly put on the object holder and the tissue in the frozen OCT/muscle block is stood up in the OCT---oriented to stand up so that cross sections can be cut. The OCT will quickly freeze holding the block in its cutting position. 4. The standing block now needs to be bolstered with OCT for cutting. OCT is added in discrete steps and quickly frozen, building up by degrees a rectangle of frozen OCT. The object holder with the block on it is then put in the cutting position. It is trimmed to make all edges of the rectangle surrounding the tissue straight and clean and the corners square. The block is positioned in the cryostat to make the long edge of the block parallel with the knife edge. Sections are cut at 8 microns to nearly fill the slide, and one section, 10 microns thick, completes the filling of the slide. Thanks, Mauricio. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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From lesley <@t> vancouverbc.net Wed Nov 12 09:11:20 2003 From: lesley <@t> vancouverbc.net (Lesley Weston) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Torn tissues followup In-Reply-To: <5.1.0.14.0.20031111152436.00a2a5f0@mail.jcu.edu.au> Message-ID: As well as the bones, perhaps the scales are causing trouble too; they are also calcified. Either way, decalcification should solve the problem. Lesley Weston. on 10/11/2003 9:34 PM, Laurie Reilly at Laurie.Reilly@jcu.edu.au wrote: > Julien, > You haven't mentioned anything about decalcifying these fish. > > When we are to section fish, they are usually fixed in Davidson's or > Bouin's, both of which contain acids which will decalcify > the bones in the fish. Maybe your torn tissues are caused by the calcified > bones damaging your microtome knife. > > Regards, Laurie. > > At 02:04 PM 11/10/03 +0000, Julien Lambrey de Souza wrote: >> Hello to all histonetters, >> >> I am having problems with torn tissues and I have already posted a message >> asking what may be the causes. Thanks for the various replies. Most of you >> aksed me more details about the procedure in order to try and define a >> problem. so here goes: >> >> Small fish (5cm) where fixed in formalin and then preserved in 70% EtOh >> >> Processing is done on an automated processor >> 2 hours in 95%alcohol >> 2 hours in 100% >> 3 hours in xylene >> 1 hour in wax (paraplus) >> 1 hour in wax with vacuum. >> >> Embedding is done with paraplus wax on a histocenter II. >> >> Sectioning is done at 7 microns, transversally to body length. >> >> To mount secions on slides, 2 methods are used. The first is a heated >> waterbath to unrinkle the tape. But this methods complicates recognition >> of sections to the fish length. That's why we prefer to lay the tapes on >> a black cardboard paper, put some water on slides, put the tape on the >> slide and then heat slightly the slide on a slide warmer so the tape >> expands to unrinkle on the buble of water. This technique has worked fine >> up to now and enables us to keep track of how far we are in the fishe's body. >> >> Staining is done on an automated stainer >> Xylene 3 min (x3) >> 100% alcohol 2 min >> 100% 3 min >> 95% 2 min (x2) >> 70% 2 min >> Water 2 min >> Hematoxylin 4 min >> Water 2 min >> Bluing reagent 2 min >> Water 3 min >> 95% 30 sec >> Eosine 2 min >> 95% 1 min (x2) >> 100% 1 min >> 100% 2 min >> Xylene 3 min (x3) >> >> Coverslips are mounted automatically. >> >> So there it is. I don't think the tearing is due to microtome blade since >> I use a new disposable blade each time I have a doubt. >> So, according to the comments we received up to now, the parameters I'm >> going to change are the processing times in alcohol and xylene. I am going >> to lower them but raise the time in parafin. I have to check the parafin >> temperature as I was told an excessively hot wax will not penetrate as well. >> >> Also I'm going to try and reduce slide warming temperature but leave them >> dry longer, since wet sections may slide off during staining. >> >> So, if you have any further suggestion to help me solve my problem, all >> comments are welcome. >> >> Chears, >> >> Julien De souza. >> >> _________________________________________________________________ >> Add photos to your messages with MSN 8. Get 2 months FREE*. >> http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin.m >> sn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Mr.Laurie Reilly Ph 07 4781 4468 > Physiology & Pharmacology Fax 07 4779 1526 > Aust.Inst.of Tropical Vet.& Animal Sc. > James Cook University > Townsville Qld. > 4811 laurie.reilly@jcu.edu.au > > Australia. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Nov 12 10:16:40 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] GFP In-Reply-To: <148.1c39f5aa.2ce19668@aol.com> Message-ID: <3.0.6.32.20031112091640.00bd5ed0@gemini.msu.montana.edu> This has been discussed on Histonet, go to Archives and search, look for Donna Montague, she has great success with paraffin work unless you are doing frozen sections. Formalin and 4% paraformaldehyde will work as a fixatives. You need to know the difference between autofluorescence and GFP, hopefully the GFP will fluoresce at maximum level to overcome the autofluorescence. So you need to have a control, naive or normal (contains no GFP) rat duodenum, fixed at same time as your experimental animal, and look at this tissue so you know how autoflourescence appears. Do not use an H&E, GFP is fluorescent and you need to examine without any staining to see GFP, hopefully not a wild type GFP but eGFP, or enhanced GFP. At 08:33 PM 11/10/2003 EST, you wrote: >Hello, > I need to use this procedure to visualize a vector that will be injected >into rat duodenum. I would appreciate any hints on fixation and staining >procedures procedure that anyone may want to share. > lin > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From pedro.louro <@t> spcorp.com Wed Nov 12 10:17:40 2003 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] looking for Mary Georger Message-ID: <4508920F80C0D411B90200508BF9A9F4030A5162@LAFMSG30.us.schp.com> Sorry about posting this..but I'm trying to get in touch with Mary Georger Please respond to me if possible.. Thanks ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031112/5b2ff99b/attachment.htm From gcallis <@t> montana.edu Wed Nov 12 10:37:21 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] heart IHC work In-Reply-To: Message-ID: <3.0.6.32.20031112093721.00bd5ed0@gemini.msu.montana.edu> Cut frozen sections, then fix for IHC. Why compromise the tissue twice, frozen then thaw, fix and process? That is how I interpret this message, correct? At 02:13 PM 11/11/2003 -0700, you wrote: >Friends, >I am involved in a project that requires doing IHC for Lamin A/C on heart >tissue. If anyone has experience with this please tell me all you know. >Also, since most of this is DNA work they have pieces of fresh heart frozen >at -70 dC. What would be the best way to fix and process the frozen heart >so that it would be well preserved for IHC. >Thank you for advise in this matter >Patsy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Ronnie_Houston <@t> bshsi.com Wed Nov 12 11:04:37 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] heart IHC work Message-ID: <530361BF03351B4CAE5270A05D3037B5FE0529@bsrexms01.BSHSIR.COM> Patsy, My recollection is that we did lamin a/c immunofluorescence on frozen sections of skeletal muscle (antibody from Chemicon), secondary Alexa dyes from Molecular Probes, mount in Vectashield with DAPI. Excellent results Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Wednesday, November 12, 2003 11:37 AM To: pruegg@colobio.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] heart IHC work Cut frozen sections, then fix for IHC. Why compromise the tissue twice, frozen then thaw, fix and process? That is how I interpret this message, correct? At 02:13 PM 11/11/2003 -0700, you wrote: >Friends, >I am involved in a project that requires doing IHC for Lamin A/C on heart >tissue. If anyone has experience with this please tell me all you know. >Also, since most of this is DNA work they have pieces of fresh heart frozen >at -70 dC. What would be the best way to fix and process the frozen heart >so that it would be well preserved for IHC. >Thank you for advise in this matter >Patsy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From pedro.louro <@t> spcorp.com Wed Nov 12 11:11:11 2003 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] eye fixatives Message-ID: <4508920F80C0D411B90200508BF9A9F4030A5164@LAFMSG30.us.schp.com> I'm looking for information on any procedure using Davidson's Fixative for eyes for both Light and Electron Microscopy Any information would be greatly appreciated Thanks, Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031112/2f899548/attachment.htm From hborgeri <@t> wfubmc.edu Wed Nov 12 11:38:22 2003 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:22:11 2005 Subject: [Histonet] Hard Tissue Committee members Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8E94B@EXCHVS2.medctr.ad.wfubmc.edu> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 3781 bytes Desc: image001.jpg Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031112/6d8f47c1/attachment.jpg -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 15492 bytes Desc: image002.gif Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031112/6d8f47c1/attachment.gif From d.gregg <@t> juno.com Wed Nov 12 13:01:09 2003 From: d.gregg <@t> juno.com (Douglas A Gregg) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Finding GFP Message-ID: <20031112.140218.1752.2.d.gregg@juno.com> Hello, I just went through this problem with and adenovirus expressing GFP. I could not find it in PLP fixed paraffin embedded tissue. I had been able to find a stronger signal by FA with a vesicular stomatitis virus-GFP. However, I had great result with avidin biotin immunostaining with a MAb to GFP made by Zymed at 1:75 with Dako antigen retrieval (citrate). I used Vector red and got very strong red staining. I could see even more of the weaker staining cells by using an FA scope and Texas red filters. Good luck. This should make life easier. Douglas Gregg Plum Island Animal Disease Center USDA Greenport, NY 11944 From histolog <@t> fcv.unl.edu.ar Wed Nov 12 13:35:15 2003 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Fixative Message-ID: <004601c3a954$1bdfbaa0$5a0cd2aa@unl.edu.ar> Recently I have read a paper (Lab Invest 2003, 83:1427-1435) in the one that the authors present a fixer that would allow the preservation of Macromolecules (DNA, RNA, and proteins) in tissues avoiding the necessity to use immediate freezing sections. The fixative presented is a misture of methanol and polyethylene glycol comercialized by Sakura (UMFIX). Somebody has used it or had used mixtures of methanol and polyethylene glycol ??? Thanks ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From JWebb01 <@t> JPSHealthNetwork.org Wed Nov 12 13:47:46 2003 From: JWebb01 <@t> JPSHealthNetwork.org (Webb, Judith) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] slide labeler Message-ID: Has anyone had a recent problem with colormark+ slides on the TBS SureMark Labeler? The label end of the slide seems to have a different texture? coating? causing the etching to be hard to read. Thanks for your help. Judy Webb Technical Coordinator Anatomic Pathology John Peter Smith Hospital 1500 S. Main Ft. Worth Texas, 76104 817-927-1024 817-927-3630 fax From jkiernan <@t> uwo.ca Wed Nov 12 13:57:02 2003 From: jkiernan <@t> uwo.ca (J. A. Kiernan) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] need help for epon References: <134.27953bea.2ce24c05@aol.com> Message-ID: <3FB2908E.47C72420@uwo.ca> You would not expect a trichrome method for paraffin sections to work properly after glutaraldehyde, osmium tetroxide and epoxy resin embedding. Both the fixatives interfere with amino groups (to which the dyes in a trichrome are attracted, and both glutaraldehyde fixation and the cross-linked resin interfere with penetration of the larger dye molecules in the mixture. 5um is thicker than usual for such sections. If you cut at 0.5 or 1 um you will see all the structural details with a one-colour stain such as alkaline toluidine blue. The staining intensity can be increased by first putting a drop of concentrated sulphuric acid on top of the section for about 20 seconds then rinsing off with tap water before staining. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ______________________________________ Myri37@aol.com wrote: > > hello > i have embedded thin tissue in epon, after postfixation with > osmium > i stuck sections of 5 um on slides with haupt's solution, > deplastized in ethoxide of sodium in heat for 30 minutes > and after that stained Goldner's trichrome > tissue was not coloured, and i dont understand why, is it > because of osmium ? > pleaze do you have a protocol of Goldner's trichrome especially > for epon not for parrafin sections, or another stain to show > collagen and mineralized tissu ? > Myriam > Natural implant > France -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From cfavara <@t> niaid.nih.gov Wed Nov 12 14:22:07 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] eye fixatives Message-ID: We do lots of eyes and use Davidson's for light what do you want to know? c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Louro, Pedro [mailto:pedro.louro@spcorp.com] Sent: Wednesday, November 12, 2003 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eye fixatives I'm looking for information on any procedure using Davidson's Fixative for eyes for both Light and Electron Microscopy Any information would be greatly appreciated Thanks, Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031112/bcaccd8e/attachment.htm From nmuvarak <@t> facstaff.wisc.edu Wed Nov 12 14:56:36 2003 From: nmuvarak <@t> facstaff.wisc.edu (NIDAL E MUVARAK) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Drying of Cryosections Message-ID: Hi everyone. I have a quick question. I've been getting some strange results while staining for MMP-2 and MMP-9 in mouse lungs. I section the tissue, allow them to get to room temp, then I fix them in cold acetone for 10 min. I air-dry the slides for 30 min before staining. And when I stain I barely get any positive even in the positive controls, but when I do the same as above except after fixation I allow the slides to dry overnight, the following day I get really good staining, specific to blood vessels and airways, where MMPs are found. Does anyone have an explanation for that? Am I getting a false positive? Thank you for your time. Regards, Nidal E Muvarak Associate Research Specialist Vascular Tissue Biomechanics Laboratory Department of Biomedical Engineering University of Wisconsin-Madison 1550 Engineering Dr.; Rm. 2158 Madison, WI 53706-1609 Lab: (608) 265-8921; Office: (608) 265-4205; Home: (608) 256-7934; Cell: (608) 332-6068 http://vtb.bme.wisc.edu From mark.lewis <@t> thermo.com Wed Nov 12 15:26:39 2003 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Equipment resalers Message-ID: I need the name of someone that buys and sells used equipment. Please contact Caustice at 416-752-5730 I am not sure of the spelling of his name. Thank you Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com From Ngilman <@t> nbhd.org Wed Nov 12 15:33:01 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] trend/workload tracking Message-ID: I need some suggestions from you good folk. How do you identify what tech does what jobs? For example, I have two tech's who work overnight in our lab. I want to find out who cuts which cases, who stains H & E's, which special stains each does, etc. As it is now, they know what needs to be done and they get it done, but sometimes the finished slides are not of the best quality, and my doc's want to know who does what. I thought of a form where they list the cases they cut, stain, coverslip, etc, but I'd like some feedback from what the rest of the histo world is doing. TIA Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031112/5c36e479/attachment.htm From pruegg <@t> colobio.com Wed Nov 12 16:47:51 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] heart IHC work In-Reply-To: Message-ID: I will definitely make frozen sections from the frozen heart but I was just wondering what would be a good way to then process some of it into paraffin for IHC. I used to do this with the frozen OCT block by just placing the block into cold PBS and then eventually into RT fixative after a while, is this a proper method to preserve antigens? Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Patsy Ruegg Sent: Tuesday, November 11, 2003 2:14 PM To: histonet@pathology.swmed.edu Subject: [Histonet] heart IHC work Friends, I am involved in a project that requires doing IHC for Lamin A/C on heart tissue. If anyone has experience with this please tell me all you know. Also, since most of this is DNA work they have pieces of fresh heart frozen at -70 dC. What would be the best way to fix and process the frozen heart so that it would be well preserved for IHC. Thank you for advise in this matter Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kab35 <@t> cornell.edu Wed Nov 12 17:27:27 2003 From: kab35 <@t> cornell.edu (Kathie Berghorn) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] GFP and counterstaining Message-ID: Histonetters, I will be looking at cells that have been transfected with GFP and need to know what part of the cell is expressing GFP so I want to counterstain the cells. Will this work? If so, what counterstain should I use? Thanking you in advance, Kathie From Jason.PALMER <@t> svhm.org.au Wed Nov 12 23:47:36 2003 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] monoclonal beta galactosidase Abs for IHC on FFPE tissue Message-ID: Hi there. I've been using a polyclonal beta gal Ab (Abcam 616) for immunos on FFPE tissue, but have been getting a lot of background in the tissue I really want to get a result in, and so now am thinking of trying a monoclonal. I couldn't find one that is has been used successfully on FFPE tissue, as far as I could tell, but am probably going to try a Zymed one (03-2100) that at least is known to work for immunos on frozen sections. Has anybody used this, or other beta gal monoclonals on FFPE tissue with any success? Much obliged if you can help, Cheers, Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: palmerj@svhm.org.au -------------- next part -------------- Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. From lpwenk <@t> covad.net Thu Nov 13 04:20:58 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] trend/workload tracking References: Message-ID: <004501c3a9cf$ed9698e0$bf28a544@hppav> EMBEDDING: We used to cut up colored paper, 2-3 mm square, and put it in the top of the cassette after filling it with paraffin during embedding. Each of us had a different color. That way, we knew who embedded which cassette, in case there was a problem. SECTIONING: Everyone initialed the slides they sectioned. Since the paper label covered it up, we just looked at the back of the slide, and viewed the initials in reverse. H&E: Any chance - whoever cuts the case, stains the case? That's what we did. SPECIAL STAINS: Each one has a sheet of paper, and lists the case numbers they stain. Or there is one central sheet, and the case number gets written down, along with the name of the stain, and they initial which case is theirs. COVERSLIPPING: If you stain it, you coverslip it - that might be the easiest, if possible. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: Noreen Gilman To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 12, 2003 4:33 PM Subject: [Histonet] trend/workload tracking I need some suggestions from you good folk. How do you identify what tech does what jobs? For example, I have two tech's who work overnight in our lab. I want to find out who cuts which cases, who stains H & E's, which special stains each does, etc. As it is now, they know what needs to be done and they get it done, but sometimes the finished slides are not of the best quality, and my doc's want to know who does what. I thought of a form where they list the cases they cut, stain, coverslip, etc, but I'd like some feedback from what the rest of the histo world is doing. TIA Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/ccdce877/attachment.htm From livieira <@t> ualg.pt Thu Nov 13 06:45:29 2003 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Sudan Black and Oil red O Basic questions Message-ID: <004c01c3a9e4$05331fa0$2914100a@labhistologia> Helo Everyone, I will start a work with lipids stain and I have some basic questions. In the protocol for Sudan Black and Oil Red O say: Solution of Sudan Black 0.4% in ethanol 70%: dissolve and place at 37?C for 12 hours, wait to get cold and filter (solution stable for 15 days) And the same for the solution of oil red O, but in this case I don?t have any indication of the validity. And my questions are: Is necessary that the solutions stay at 37?C for 12 hours "exactly" or is possible reduce this time (or extend?) and wath are your opinion about the validity and conditions of storage? Tanks in advance Lina Vieira -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/88303598/attachment.htm From Ngilman <@t> nbhd.org Thu Nov 13 07:10:08 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] trend/workload tracking Message-ID: I want to thank all of you in histoland for your ideas on workload tracking. You have given me several solutions that I can adapt for use in our lab. Thanks again! Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/4c20fa35/attachment.htm From fcs <@t> uevora.pt Thu Nov 13 07:45:04 2003 From: fcs <@t> uevora.pt (Fernando Capela e Silva) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Oxidized LDL Message-ID: <002e01c3a9ec$58bc0940$d0d988c1@uevora.pt> I would lik receive information about some antibody for Oxidized LDL, for paraffin sections of pig artery formalin fixed With compliments * * * * * * * * * * * * * * * * * * * * * * * * * Fernando Capela e Silva Laborat?rio de Morfologia Funcional UBC - Unidade de Biologia da Conserva??o Departamento de Biologia - Universidade de ?vora Apartado 94 7002-554 ?vora PORTUGAL Phone: +351-266 760 881 Fax: +351-266 760 914 Email: fcs@uevora.pt http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/52b4c11d/attachment.htm From JWebb01 <@t> JPSHealthNetwork.org Thu Nov 13 09:48:26 2003 From: JWebb01 <@t> JPSHealthNetwork.org (Webb, Judith) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] slides Message-ID: Thanks to Erie Scientific for your quick response to my slide problem. You are the best! Judy Webb Technical Coordinator Anatomic Pathology John Peter Smith Hospital 1500 S. Main Ft. Worth Texas, 76104 817-927-1024 817-927-3630 fax From alust1 <@t> hotmail.com Thu Nov 13 11:50:29 2003 From: alust1 <@t> hotmail.com (Andrew Lust) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Trypsin recipe's Message-ID: Dear Histonetters Does anybody have a good recipe for Trypsin? Wondering if a trypsin treatment will help in my anitbody staining adventure. thanks again Andrew _________________________________________________________________ Is your computer infected with a virus? Find out with a FREE computer virus scan from McAfee. Take the FreeScan now! http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From tpmorken <@t> labvision.com Thu Nov 13 11:54:21 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Tutorial program now offers CEU credits through NSH Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CCF3@usca0082k03.rallansci.apogent.com> Histonetters, A while ago I mentioned that Lab Vision is offering free Histotechnology tutorials on our website. I am now pleased to announce that CEU credits for these tutorials are available through the National Society for Histotechnology (USA, see www.nsh.org).). The names of all those who complete the courses offered will be forwarded to the NSH for credit. Those who are members of NSH will receive credit certificates annually (at the end of the year). Those who are not members of NSH will need to request that the certificates be sent to them (There is no charge to participants for these credits). Also, the professional societies of many other countries will accept NSH credits for their continuing education needs. Please check with your local associations to determine eligibility. At present one tutorial is posted. In the near future we will post more tutorials as they become available. Please visit the website to check it out: http://www.labvision.com/indexTutorial.cfm Tim Morken Lab Vision / NeoMarkers www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/3235d4c5/attachment.htm From cfavara <@t> niaid.nih.gov Thu Nov 13 12:23:20 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Tutorial program now offers CEU credits through NS H Message-ID: What a FABULOUS thing to do!!! Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Thursday, November 13, 2003 10:54 AM To: Histonet Subject: [Histonet] Tutorial program now offers CEU credits through NSH Histonetters, A while ago I mentioned that Lab Vision is offering free Histotechnology tutorials on our website. I am now pleased to announce that CEU credits for these tutorials are available through the National Society for Histotechnology (USA, see www.nsh.org).). The names of all those who complete the courses offered will be forwarded to the NSH for credit. Those who are members of NSH will receive credit certificates annually (at the end of the year). Those who are not members of NSH will need to request that the certificates be sent to them (There is no charge to participants for these credits). Also, the professional societies of many other countries will accept NSH credits for their continuing education needs. Please check with your local associations to determine eligibility. At present one tutorial is posted. In the near future we will post more tutorials as they become available. Please visit the website to check it out: http://www.labvision.com/indexTutorial.cfm Tim Morken Lab Vision / NeoMarkers www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/1575c625/attachment.htm From kspencer <@t> utmem.edu Thu Nov 13 13:03:18 2003 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] retrograde tracers Message-ID: <0AFFA27F-160C-11D8-8E4A-000393967904@utmem.edu> Has anyone out there done in vivo retrograde tracing with a fluorescent dye for subsequent laser capture microdissection? If so please contact me. Thanks, Kathleen Spencer LCM Supervisor/ Lab Manager UTHSC 901.448.3720 -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: text/enriched Size: 244 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/e17a69f9/attachment.bin From jlambrey <@t> hotmail.com Thu Nov 13 13:21:42 2003 From: jlambrey <@t> hotmail.com (Julien Lambrey de Souza) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] still having tissue tears Message-ID: Hello all, A few days ago, I wrote to the histonet community to share a problem I am having. I am trying to obtain nice sections of fish but when I am cutting, the tissue keeps tearing. I have excluded microtome errors since I have tried different blade angles and am using disposable blades (a new one each time). I also keep my parafin blocks cold on ice. I think the problem more likely come from the processing. Fish (juveniles of a few centimeters long) were previousely fixed in formalin and then transfered to 70% alcohol (a few months ago). I cut a centimeter thick piece transversally through the fish. This piece is then decalcified for 30 minutes. I have tried two processing protocols. The first starts in 95% EtoH (70% being skipped since the fish were stored in it)for 1 hour (x2), then 100% EToH for 1 hour (x2), 3 consecutive Xylen baths for 1 hour each and two parafin baths of an hour each, the second being with vacuum. Following recomendations given to me after the first histonet message, a second processing protocol was done: 95% EToH (30 minutes), 100% ETOH (30 minutes), two xylen baths for 30 minutes each, another xylen bath for 45 minutes, parafin for 1H and parafin again with vacuum for 1H30min. Both resulted in tissue tearing. Does anybody see what I am doing wrong? Does anybody know of a good fish section processing protocol? I have to get this one wright to eventually process smaller and smaller fish, finally ending with larvae processing. After cutting, the H&E coloration is really good. Unfortunatly it hasn't been done on good sections. Any help would be greatly appreciated. Chears, Julien De Souza University of Quebec at Rimouski. _________________________________________________________________ Add photos to your messages with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From sharon.willman <@t> bms.com Thu Nov 13 13:23:25 2003 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] (Histonet) Immunostaining Scale Message-ID: <3FB3DA2D.C42A9D58@bms.com> Hi, My Pathologist would like to know if there is a scale for determining intensity of immuno stains. For example (1-5) and what is the criteria for determing this. Any help would be most appreciated. Thanks, Sharon Willman From pruegg <@t> colobio.com Thu Nov 13 13:37:40 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] DNA extraction from wax sections In-Reply-To: Message-ID: Isolation of DNA from paraffin-embedded tissue DNeasy Tissue Kit Handbook 05/2002 Notes: * lysis time will vary from sample to sample depending on the type of tissue processed. * Yields will depend both on the size and the age of the sample processed. Reduced yields compared to fresh or frozen tissues are to be expected. Therefore, eluting the DNA in 50-100 ul buffer AE is recommended. * 1. Place a small piece (not more than 25 mg) of paraffin-embedded tissue in a 2 ml microcentrifuge tube. * 2. Add 1200 ul xylene. Vortex vigorously. * 3. Centrifuge at full speed for 5 min. at RT. * 4. Remove supernatant by pipetting. Do not remove any of the pellet. * 5. Add 1200 ul absolute ethanol to the pellet to remove residual xylene and mix gently by vortexing. * 6. Centrifuge at full speed for 5 min at room temperature. * 7. Carefully remove the ethanol by pipetting. Do not remove any of the pellet. * 8. Repeat steps 5-7 once. * 9. Incubate the open microcentrifuge tube at 37 dC for 10-15 min until the ethanol has evaporated. * 10. Resuspend the tissue pellet in 180 ul Buffer ATL and continue with the "DNeasy Protocol for Tissues". * From this point the tissue can be treated as if it were fresh. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Michele Ellender Sent: Thursday, November 06, 2003 8:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] DNA extraction from wax sections Dear all, Does anyone out there have a protocol to extract DNA from sections of formalin fixed wax embedded mouse tissues? I've been asked to microdissect GI tumours from wax sections and extract the DNA for use in PCR techniques. Hope someone can help. Many thanks, Michele Dr Michele Ellender Radiation Effects Department, NRPB, Chilton, Didcot, Oxon. OX11 0RQ. UK michele.ellender@nrpb.org This e-mail transmission is strictly confidential and intended solely for the person or organisation to whom it is addressed. It may contain privileged and confidential information and if you are not the intended recipient, please do not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, please notify the sender as soon as possible and delete the message. Please note that NRPB monitors incoming and outgoing e-mail for compliance with its Acceptable Use Policy. This will include scanning incoming e-mails to detect viruses and key-words and may in some circumstances result in the manual monitoring of the content of messages. National Radiological Protection Board E-mail: nrpb@nrpb.org Web site: www.nrpb.org -------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Nov 13 13:58:24 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] still having tissue tears In-Reply-To: Message-ID: <3.0.6.32.20031113125824.00bcfc30@gemini.msu.montana.edu> Buy the VIR (Veterinary, Industry and Research) Animal Processing Manual from National Society for Histotechnology, there are several fish processing schedules, including fixation. www.NSH.org, the website will tell how to purchase this manaul At 07:21 PM 11/13/2003 +0000, you wrote: >Hello all, > >A few days ago, I wrote to the histonet community to share a problem I am >having. >I am trying to obtain nice sections of fish but when I am cutting, the >tissue keeps tearing. I have excluded microtome errors since I have tried >different blade angles and am using disposable blades (a new one each time). >I also keep my parafin blocks cold on ice. > >I think the problem more likely come from the processing. > >Fish (juveniles of a few centimeters long) were previousely fixed in >formalin and then transfered to 70% alcohol (a few months ago). > >I cut a centimeter thick piece transversally through the fish. >This piece is then decalcified for 30 minutes. > >I have tried two processing protocols. >The first starts in 95% EtoH (70% being skipped since the fish were stored >in it)for 1 hour (x2), then 100% EToH for 1 hour (x2), 3 consecutive Xylen >baths for 1 hour each and two parafin baths of an hour each, the second >being with vacuum. > >Following recomendations given to me after the first histonet message, a >second processing protocol was done: 95% EToH (30 minutes), 100% ETOH (30 >minutes), two xylen baths for 30 minutes each, another xylen bath for 45 >minutes, parafin for 1H and parafin again with vacuum for 1H30min. > >Both resulted in tissue tearing. >Does anybody see what I am doing wrong? Does anybody know of a good fish >section processing protocol? I have to get this one wright to eventually >process smaller and smaller fish, finally ending with larvae processing. > >After cutting, the H&E coloration is really good. Unfortunatly it hasn't >been done on good sections. > >Any help would be greatly appreciated. > >Chears, >Julien De Souza >University of Quebec at Rimouski. > >_________________________________________________________________ >Add photos to your messages with MSN 8. Get 2 months FREE*. >http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin .msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From cfavara <@t> niaid.nih.gov Thu Nov 13 14:09:44 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] still having tissue tears Message-ID: When you first face the block how does the tissue look? Do they seem dry? I am not clear as to what you mean by tearing. Is it like a large section of tissue is missing or is there just a streak across the section? It might be more helpful if you could take a picture and post so we could see what the problem is. Just a thought. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Julien Lambrey de Souza [mailto:jlambrey@hotmail.com] Sent: Thursday, November 13, 2003 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] still having tissue tears Hello all, A few days ago, I wrote to the histonet community to share a problem I am having. I am trying to obtain nice sections of fish but when I am cutting, the tissue keeps tearing. I have excluded microtome errors since I have tried different blade angles and am using disposable blades (a new one each time). I also keep my parafin blocks cold on ice. I think the problem more likely come from the processing. Fish (juveniles of a few centimeters long) were previousely fixed in formalin and then transfered to 70% alcohol (a few months ago). I cut a centimeter thick piece transversally through the fish. This piece is then decalcified for 30 minutes. I have tried two processing protocols. The first starts in 95% EtoH (70% being skipped since the fish were stored in it)for 1 hour (x2), then 100% EToH for 1 hour (x2), 3 consecutive Xylen baths for 1 hour each and two parafin baths of an hour each, the second being with vacuum. Following recomendations given to me after the first histonet message, a second processing protocol was done: 95% EToH (30 minutes), 100% ETOH (30 minutes), two xylen baths for 30 minutes each, another xylen bath for 45 minutes, parafin for 1H and parafin again with vacuum for 1H30min. Both resulted in tissue tearing. Does anybody see what I am doing wrong? Does anybody know of a good fish section processing protocol? I have to get this one wright to eventually process smaller and smaller fish, finally ending with larvae processing. After cutting, the H&E coloration is really good. Unfortunatly it hasn't been done on good sections. Any help would be greatly appreciated. Chears, Julien De Souza University of Quebec at Rimouski. _________________________________________________________________ Add photos to your messages with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin. msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGordon <@t> cellmarque.com Thu Nov 13 14:11:36 2003 From: JGordon <@t> cellmarque.com (Jeff Gordon) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] (Histonet) Immunostaining Scale Message-ID: Sharon, there is a current scale for immunostaining intensity. It is expressed as a fraction, with the numerator being a number from 1-4 describing the intensity of the primary stain and the denominator being a number from 1-4 describing the background intensity. For example, an optimal stain would read 4/1, meaning it has a strong primary stain (4) and absolute minimal background (1), and the next best stain would be a 3/1, and so forth. Usually any background that is over 1 is unacceptable, so even a 4/2, which would show good strong staining of the primary antibody but slightly higher than normal background is unacceptable. This is the only scoring of immunostaining that I am aware of. To show you a sample of our scoring of stains chart based on different pretreatments of antibodies, check this link: http://www.cellmarque.com/2000/Pages/pretreatmentcomparison.html I hope that this helps. Jeff Gordon Cell Marque Corp. 1-800-665-7284, Ext. 12 -----Original Message----- From: Sharon E Willman [mailto:sharon.willman@bms.com] Sent: Thursday, November 13, 2003 1:23 PM To: histonet@pathology.swmed.edu Subject: [Histonet] (Histonet) Immunostaining Scale Hi, My Pathologist would like to know if there is a scale for determining intensity of immuno stains. For example (1-5) and what is the criteria for determing this. Any help would be most appreciated. Thanks, Sharon Willman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Thu Nov 13 14:25:22 2003 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Cassette labeler that interfaces with Meditech Message-ID: I am looking for a cassette labeler that will interface with the Meditech Anatomic Pathology Module, Version 4.9. If any Histonetters know of a labeler, would you please contact me with the information. Vendors welcome. Thanks in advance, Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/3a14bf8e/attachment.htm From bucana <@t> audumla.mdacc.tmc.edu Thu Nov 13 14:29:14 2003 From: bucana <@t> audumla.mdacc.tmc.edu (Corazon D. Bucana) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] thick frozen sections Message-ID: <5.1.1.6.0.20031113142137.00a7d6b8@audumla.mdacc.tmc.edu> I would appreciate any suggestions on cutting 100 micron frozen sections of mouse brain for immunohistochemistry. our sections either crack or show a lot of chatter. Thanks, Cora Bucana From tflore <@t> lsuhsc.edu Thu Nov 13 14:46:42 2003 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] RE: [Microscopy] ? experience with Drukker diamond knives Message-ID: Peter, no I have never heard of Drukker diamond knives. But I have used the resharpening of DIATOME Knives which are sold by Electron Microscopy Science. If you purchase a knife from them (DIATOME) I believe EMS guarantees 6 sharpenings. If the original diamond knife is not from EMS, EMS will give you a credit of ? $$ towards your new DIATOME knife. Our resharpened diamond knives always return like new but a lot less. Teresa Flores LSUHSC New Orleans, LA -----Original Message----- From: PETER HEIMANN [mailto:peter.heimann@uni-bielefeld.de] Sent: Thursday, November 13, 2003 8:29 AM To: Microscopy@MSA.Microscopy.Com Subject: [Microscopy] ? experience with Drukker diamond knives ---------------------------------------------------------------------------- -- The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.msa.microscopy.com/MicroscopyListserver On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html ---------------------------------------------------------------------------- --- Hi, has anybody longer experience with diamond knives from Drukker (The Netherlands / Europe)? Can You recommend them? Have you ever had re-sharpened a knive? Thanks for a short informal and offline reply to peter.heimann@uni-bielefeld.de Peter Heimann -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031113/35098d1b/attachment.htm From gcallis <@t> montana.edu Thu Nov 13 17:40:53 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] REPLY: Tearing of fish tissue In-Reply-To: Message-ID: <3.0.6.32.20031113164053.00bd15f8@gemini.msu.montana.edu> It could be that the decalcification is not complete. Trim block or take an already sectioned block, place it face down in decalcifier (formic acid or HCl, your commerical decalcifier) for a 5 - 10 minutes, rinse it well with tap water, cool block. Do not change reorientation of block holder so you can BE SURE to get the first sections that come off the block. DO NOT RETRIM, you must have the surface decalcified tissue section, and that is only for a few micrometers into that block face. If you block face, e.g. the tissue looks chalky, opaque, you may have poor decalcification, poor dehydration OR both. Go back and look at your processing schedule and decalcification protocol again. Rinsing the acid off the block face will save metal parts on microtome from corrosion. Good luck, At 03:09 PM 11/13/2003 -0500, you wrote: >When you first face the block how does the tissue look? Do they seem dry? I >am not clear as to what you mean by tearing. Is it like a large section of >tissue is missing or is there just a streak across the section? > >It might be more helpful if you could take a picture and post so we could >see what the problem is. > >Just a thought. >c > >Cynthia Favara >NIAID/NIH/RML/LPVD >903 South 4th Street >Hamilton, MT 59840 >406-363-9317 > > >-----Original Message----- >From: Julien Lambrey de Souza [mailto:jlambrey@hotmail.com] >Sent: Thursday, November 13, 2003 12:22 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] still having tissue tears > > >Hello all, > >A few days ago, I wrote to the histonet community to share a problem I am >having. >I am trying to obtain nice sections of fish but when I am cutting, the >tissue keeps tearing. I have excluded microtome errors since I have tried >different blade angles and am using disposable blades (a new one each time). > >I also keep my parafin blocks cold on ice. > >I think the problem more likely come from the processing. > >Fish (juveniles of a few centimeters long) were previousely fixed in >formalin and then transfered to 70% alcohol (a few months ago). > >I cut a centimeter thick piece transversally through the fish. >This piece is then decalcified for 30 minutes. > >I have tried two processing protocols. >The first starts in 95% EtoH (70% being skipped since the fish were stored >in it)for 1 hour (x2), then 100% EToH for 1 hour (x2), 3 consecutive Xylen >baths for 1 hour each and two parafin baths of an hour each, the second >being with vacuum. > >Following recomendations given to me after the first histonet message, a >second processing protocol was done: 95% EToH (30 minutes), 100% ETOH (30 >minutes), two xylen baths for 30 minutes each, another xylen bath for 45 >minutes, parafin for 1H and parafin again with vacuum for 1H30min. > >Both resulted in tissue tearing. >Does anybody see what I am doing wrong? Does anybody know of a good fish >section processing protocol? I have to get this one wright to eventually >process smaller and smaller fish, finally ending with larvae processing. > >After cutting, the H&E coloration is really good. Unfortunatly it hasn't >been done on good sections. > >Any help would be greatly appreciated. > >Chears, >Julien De Souza >University of Quebec at Rimouski. > >_________________________________________________________________ >Add photos to your messages with MSN 8. Get 2 months FREE*. >http://join.msn.com/?page=dept/features&pgmarket=en-ca&RU=http%3a%2f%2fjoin. >msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Nov 13 17:42:05 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] thick frozen sections In-Reply-To: <5.1.1.6.0.20031113142137.00a7d6b8@audumla.mdacc.tmc.edu> Message-ID: <3.0.6.32.20031113164205.00bd15f8@gemini.msu.montana.edu> A vibrating microtome may be superior for this - or you have to warm up the cryostat chamber in order to cut at warmer temperatures. At 02:29 PM 11/13/2003 -0600, you wrote: >I would appreciate any suggestions on cutting 100 micron frozen sections of >mouse brain for immunohistochemistry. our sections either crack or show a >lot of chatter. > > >Thanks, > >Cora Bucana > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From CTague <@t> ahs.llumc.edu Thu Nov 13 18:43:37 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] sentinal nodes with pigment Message-ID: a friend of mine was asking if i knew anything about sentinal nodes that have a large amount of heavy black pigment. melanoma nodes specifically, which can create a problem when diagnosing. seems to think it might have something with the radioactive tracer that is used and the pH of some of the chemicals. anyone have any idea what might cause this. i'm told that there is no problem in any other tissues, just the sentinal nodes. i, in my limited knowledge, could not answer the question so i told him i'd ask the worlds great experts.... the histonet. thanks, curt Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From Jason.PALMER <@t> svhm.org.au Fri Nov 14 00:22:48 2003 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] monoclonal beta galactosidase Abs for IHC on FFPE tissue #2 Message-ID: Hi there. Posted this message once and came out as gobbledegook in the next day's digest so I thoought I might try again ... I've been using a polyclonal beta gal Ab (Abcam 616) for immunos on FFPE tissue, but have been getting a lot of background in the tissue I really want to get a result in, and so now am thinking of trying a monoclonal. I couldn't find one that is has been used successfully on FFPE tissue, as far as I could tell, but am probably going to try a Zymed one (03-2100) that at least is known to work for immunos on frozen sections. Has anybody used this, or other beta gal monoclonals on FFPE tissue with any success? Much obliged if you can help, Cheers, Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: palmerj@svhm.org.au -------------- next part -------------- Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. From jkiernan <@t> uwo.ca Fri Nov 14 00:32:37 2003 From: jkiernan <@t> uwo.ca (J. A. Kiernan) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Immunostaining Scale In-Reply-To: Message-ID: Dear Sharon W, Jeff G and all others, If intensity of an immunostain means the darkness of the end product, this does not indicate the quantity or local concentration of the antigen unless several stringent conditions are met. With amplifying methods (PAP, ABC and the like) the intensity of the colour of the final product can be lower at sites of high antigen concentration than at at sites of low antigen concentration. The reasons are well documented in textbooks and peer-reviewed journals. The classic is probably Bigbee et al 1977 J Histochem Cytochem 25:443-447, They simply explained at least one reason why darker brown doesn't always mean more antigen. The dissociation between antigen concentration and intensity of staining is sometimes called the "Bigbee effect." Quantitation of immunostaining intensity is a worthless exercise if you do not have ways to control all the intermediate steps of the technique. Even a crude 1-4 scoring system is meaningless without rigorous criteria for all the steps in the immunostaining and the enzyme histochemistry that generates the visible result. Amplification immunohistochemistry is designed to collect Yes or No answers. All "weak" or "background" staining cries out for explanation. The notion of a simple scale of intensity of immunostaining is worthless without standards fo the procedures and the anticipated results. John A. Kiernan MB, ChB, PhD, DSc Dept of Anat & Cell Biol, U.W.O., London, Canade. _________________________________________________ On Thu, 13 Nov 2003, Jeff Gordon wrote: > Date: Thu, 13 Nov 2003 14:11:36 -0600 > From: Jeff Gordon > To: Sharon E Willman , histonet@pathology.swmed.edu > Subject: RE: [Histonet] (Histonet) Immunostaining Scale > > Sharon, there is a current scale for immunostaining intensity. It is expressed as a fraction, with the numerator being a number from 1-4 describing the intensity of the primary stain and the denominator being a number from 1-4 describing the background intensity. For example, an optimal stain would read 4/1, meaning it has a strong primary stain (4) and absolute minimal background (1), and the next best stain would be a 3/1, and so forth. Usually any background that is over 1 is unacceptable, so even a 4/2, which would show good strong staining of the primary antibody but slightly higher than normal background is unacceptable. This is the only scoring of immunostaining that I am aware of. To show you a sample of our scoring of stains chart based on different pretreatments of antibodies, check this link: http://www.cellmarque.com/2000/Pages/pretreatmentcomparison.html > > I hope that this helps. > > Jeff Gordon > Cell Marque Corp. > 1-800-665-7284, Ext. 12 > > -----Original Message----- > From: Sharon E Willman [mailto:sharon.willman@bms.com] > Sent: Thursday, November 13, 2003 1:23 PM > To: histonet@pathology.swmed.edu > Subject: [Histonet] (Histonet) Immunostaining Scale > > > Hi, > My Pathologist would like to know if there is a scale for > determining intensity of immuno stains. For example (1-5) and > what is the criteria for determing this. > > Any help would be most appreciated. > > Thanks, > Sharon . From manuelle.debock <@t> UGent.be Fri Nov 14 02:14:40 2003 From: manuelle.debock <@t> UGent.be (Manuelle De Bock) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] DBA staining for parietal cells Message-ID: <002801c3aa87$5a19e820$ed54bec1@rug.ac.be> Hello everybody, First of all I want to thank you all for helping me with my T and B cell staining problem. I got many replies and am working out some new protocols now. Thanks! I also have a new question, has someone already worked with the lectin DBA (horse gram) to color parietal cells? Could you give me some more information about that (what are the bindingplaces of this lectin on the parietal cell?) I have not worked with lectins yet. Thanks! Manuelle Manuelle De Bock, dierenarts (DVM) Laboratory of Veterinary Pathology Department of Pathology, Bacteriology and Avian Diseases Faculty of Veterinary Medicine Ghent University Salisburylaan 133 B9820 Merelbeke - Belgium Tel 0032(0)9 264 7745 Fax 0032(0)9 264 7789 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/2fc527f1/attachment.htm From TMcNemar <@t> lmhealth.org Fri Nov 14 05:22:08 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] trend/workload tracking Message-ID: <90092A4ED388D7119575006008F7112049CB40@NT_EXCHANGE> Another thought.... Each of our cutters are assigned a number (1 throught 4) that is written on each slide. We keep a log of special stains done including the case number, date, stain done, and the reactivity of the control. Each person generally cuts their own slides for the special stains that they will be doing. H&Es are done on an automatic stainer so we don't worry about that. We only have one person embedding and we know who that is on a daily basis. If there is any problem, we know who the embedder was. We never know who coverslipped what but there are times that I wish I did. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: Noreen Gilman [mailto:Ngilman@nbhd.org] Sent: Thursday, November 13, 2003 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] trend/workload tracking I want to thank all of you in histoland for your ideas on workload tracking. You have given me several solutions that I can adapt for use in our lab. Thanks again! Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/0e48a2e5/attachment.htm From JNocito <@t> Pathreflab.com Fri Nov 14 06:40:15 2003 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Tutorial program now offers CEU credits through NSH In-Reply-To: <8CD0CDC791DCC343ABB1F15298E4F3C417CCF3@usca0082k03.rallansci.apogent.com> Message-ID: Way to go Tim. Thanks a bunch. Joe -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Morken, Tim - Labvision Sent: Thursday, November 13, 2003 11:54 AM To: Histonet Subject: [Histonet] Tutorial program now offers CEU credits through NSH Histonetters, A while ago I mentioned that Lab Vision is offering free Histotechnology tutorials on our website. I am now pleased to announce that CEU credits for these tutorials are available through the National Society for Histotechnology (USA, see www.nsh.org).). The names of all those who complete the courses offered will be forwarded to the NSH for credit. Those who are members of NSH will receive credit certificates annually (at the end of the year). Those who are not members of NSH will need to request that the certificates be sent to them (There is no charge to participants for these credits). Also, the professional societies of many other countries will accept NSH credits for their continuing education needs. Please check with your local associations to determine eligibility. At present one tutorial is posted. In the near future we will post more tutorials as they become available. Please visit the website to check it out: http://www.labvision.com/indexTutorial.cfm Tim Morken Lab Vision / NeoMarkers www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/953d13a6/attachment.htm From cwscouten <@t> myneurolab.com Fri Nov 14 08:11:28 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] thick frozen sections Message-ID: I agree, on both statements. Chatter or cracking is usually too cold cutting temperature. But freezing breaks cell membranes and leaks contents regardless. If your target protein is in the cytosol, you will much better results without the freezing, sectioning at 100 microns is easy with a vibrating microtome. See the link below http://www.myneurolab.com/myneurolab/mnl_products_subcat.asp?idsubcategory=181&CatOneID=4&subcatdesc=Vibratory+Microtomes+%28Vibratome%29&catdesc=Histology+Equipment Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Thursday, November 13, 2003 5:42 PM To: Corazon D. Bucana; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] thick frozen sections A vibrating microtome may be superior for this - or you have to warm up the cryostat chamber in order to cut at warmer temperatures. At 02:29 PM 11/13/2003 -0600, you wrote: >I would appreciate any suggestions on cutting 100 micron frozen sections of >mouse brain for immunohistochemistry. our sections either crack or show a >lot of chatter. > > >Thanks, > >Cora Bucana > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From M.Bromley <@t> dgri.scot.nhs.uk Fri Nov 14 08:33:42 2003 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] mopec forceps Message-ID: <7325D637DFE2D211928800902733A7F301844F62@DGAMTBDCEMS> Has anyone in the UK got a source for Mopec forceps, I am after some of the paddle-type mesh ones suitable for thin slicing breast. I have e-mailed the company in the USA without success? Forceps type AB042 They are on page 2 in the web catalogue the 'D' type Best Wishes Mike Bromley Dumfries Royal Infirmary Scotland UK -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/3695d57a/attachment.htm From gcallis <@t> montana.edu Fri Nov 14 09:24:26 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] DBA staining for parietal cells In-Reply-To: <002801c3aa87$5a19e820$ed54bec1@rug.ac.be> Message-ID: <3.0.6.32.20031114082426.00bc8640@gemini.msu.montana.edu> There is a fabulous book on Lectin staining available through Springer Verlag, or Bios Scientific publisher. This book is a tell all on lectins plus has wonderful immunostaining protocols in general. Also go to Vectorlabs website and look at their lectin information. They tell you how to do a negative control, plus give the sugar that would be used for that control with each lectin. One has to be a bit careful when working with lectins as some will bind to other components, so high concentration of Lectin or long incubation periods should be avoided. There is a lectin buffer that some lectins require. This book has the buffer recipe. It is a good investment, if anything, just for the immunostaining protocol! Good luck. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) o thank you all for helping me > with my T and B cell staining problem. I got many replies and am working >out some new protocols now. Thanks! has someone already worked with the >lectin DBA (horse gram) to color parietal cells? Could you give me some >more information about that (what are the bindingplaces of this lectin on >the parietal cell?) I have not worked with lectins yet. Thanks! >Manuelle Manuelle De Bock, dierenarts (DVM) >Laboratory of Veterinary Pathology >Department of Pathology, Bacteriology and Avian Diseases >Faculty of Veterinary Medicine >Ghent University >Salisburylaan 133 >B9820 Merelbeke - Belgium >Tel 0032(0)9 264 7745 >Fax 0032(0)9 264 7789 From CTague <@t> ahs.llumc.edu Fri Nov 14 09:32:31 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] sentinal nodes with pigment Message-ID: i guess i wrote that wrong, it's not really a pigment but more like an artifact. i think you're all smart enought to have figured that out though. thanks for you input, curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tague, Curtis Sent: Thursday, November 13, 2003 16:44 To: Histonet (E-mail) Subject: [Histonet] sentinal nodes with pigment a friend of mine was asking if i knew anything about sentinal nodes that have a large amount of heavy black pigment. melanoma nodes specifically, which can create a problem when diagnosing. seems to think it might have something with the radioactive tracer that is used and the pH of some of the chemicals. anyone have any idea what might cause this. i'm told that there is no problem in any other tissues, just the sentinal nodes. i, in my limited knowledge, could not answer the question so i told him i'd ask the worlds great experts.... the histonet. thanks, curt Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From leclercj <@t> vetanat.unizh.ch Fri Nov 14 10:41:18 2003 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] collagen IV Message-ID: Hi all, I am looking for antibodies to collagen IV that work in formalin-fixed paraffin embedded DOG tissue. I would be very pleased if anyone could help me! Marianne Oswald Institute for Veterinary Anatomy University of Zurich, Switzerland From Barry.R.Rittman <@t> uth.tmc.edu Fri Nov 14 10:23:42 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] DBA staining for parietal cells Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FB1C@UTHEVS3.mail.uthouston.edu> DBA (Dolichos biflorus) binds to several types of N-acetyl glucosamines. I would go to two sources. First for a history of lectins "The Lectins. Properties, Functions and Applications in Biology and Medicine" by Liener, Sharon and Goldstein. Academic Press. Published in 1986. Although this is an older text it is a excellent resource for information on all the lectins known up to that time. Another great sources is Vector Laboratories, I think still at Burlingame, CA.. They have a booklet that provides a lot of information re lectins. They do sell lectins and lectin antibodies and all those I have purchased from them have been outstanding. Some cautions If you are going to use a lectin bound to a peroxidase then these do often have a limited life span. For DBA and many other lectins there are a number of isolectins with slightly different binding properties and specificities. Few lectins bind to only one carbohydrate moiety and therefore the carbohydrate you use for negative controls must take this into account. Negative controls using specific (and highly purified carbohydrates) are critical in evaluating lectin binding. Barry 713-500-4134 6516 MD Anderson Blvd. Houston, TX. 77030 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Manuelle De Bock Sent: Friday, November 14, 2003 2:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DBA staining for parietal cells Hello everybody, First of all I want to thank you all for helping me with my T and B cell staining problem. I got many replies and am working out some new protocols now. Thanks! I also have a new question, has someone already worked with the lectin DBA (horse gram) to color parietal cells? Could you give me some more information about that (what are the bindingplaces of this lectin on the parietal cell?) I have not worked with lectins yet. Thanks! Manuelle Manuelle De Bock, dierenarts (DVM) Laboratory of Veterinary Pathology Department of Pathology, Bacteriology and Avian Diseases Faculty of Veterinary Medicine Ghent University Salisburylaan 133 B9820 Merelbeke - Belgium Tel 0032(0)9 264 7745 Fax 0032(0)9 264 7789 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/a4d548bb/attachment.htm From siksik03 <@t> comcast.net Fri Nov 14 10:27:03 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] fixatives In-Reply-To: <20031113180001.12580.46806.Mailman@swlx167.swmed.edu> References: <20031113180001.12580.46806.Mailman@swlx167.swmed.edu> Message-ID: Hi HistoNetters Dr. Ortega asked about the use of a fixative made up of of a combination of an alcohol and polyethylene glycol, and the possible advantages of using such a fixative in preserving RNA, DNA and proteins. As it happens, this topic is examined at length in Chapter 11 (Microwave stimulation of coagulant formalin-free fixatives) of the new edition of Kok & Boon's Microwaves for the Art of Microscopy. Briefly, Dr. Boon has been promoting the use of non-crosslinking, formalin-free ethyl alcohol/PEG fixatives since the early 1980's. The first of these to become commercially available was Kryofix, a Merck product (which I believe is available from E M Sciences in a slightly different form today as NeoFix- Rande Kline- can you clarify this?). Dick Dapson of Anatech and I developed a version when I was at Energy Beam Sciences which we called MicroFix. Dr. Boon has her own version now, called BoonFix Milestone has a version, called FineFix. Sakura has its version (I don't know its trade name). The theory is that the alcohol (ethyl alcohol or methyl alcohol) does the dehydration, while the PEG permeabilizes the cell membrane. BoonFix also contains acetic acid. The fixative does not crosslink proteins, so no antigen retrieval is necessary for immunohistochemistry. Preliminary testing seems to indicate that DNA and RNA preservation is far superior to formalin. I would be happy to provide more information privately to those intersted. best regards, Steven Slap ****************************** Steven E. Slap Microwave Consultant (413) 221-3678 microwaves@comcast.net ***************************** From GoodwinD <@t> pahosp.com Fri Nov 14 10:39:21 2003 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation Message-ID: <992899E9EC268548AB8DDE246AF8847316FEBF@PAHEX01.uphs.upenn.edu> > Greetings, Histonetters. > > One of my pathologists has expressed concern that antigenicity for certain > antibodies, specifically ER and PR, in breast tissue that is processed > using alcohol-formalin will be decreased. Can anyone cite a reference in > the literature that confirms or addresses this concern? Has anyone had > this experience, or does anyone know of any reason that using > alcohol-formalin (specifically Pen-Fix) would adversely affect IHC > staining? > > Thanks to all of you, > > Diana Goodwin > Supervisor, Anatomic Pathology > Preston 6 > 215-829-6532 > e-mail: goodwind@pahosp.com > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/4ba2152b/attachment.htm From angela.mcnabola.b <@t> bayer.com Fri Nov 14 10:46:12 2003 From: angela.mcnabola.b <@t> bayer.com (Angela McNabola) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Histofine Simple Stain PO(R)??? Message-ID: Hi all, Is anyone familiar with Histofine Simple Stain PO(R) made by Nichirei Co, in Japan? What is it? Can I buy some? I checked for a website, but was unsuccessful........... thanks, Angela McNabola Bayer Healthcare West Haven, CT From v.lamanna <@t> abdn.ac.uk Fri Nov 14 11:30:10 2003 From: v.lamanna <@t> abdn.ac.uk (Vincenzo La Manna) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Van gieson for frozen sections Message-ID: <41269.139.133.7.38.1068831010.squirrel@www.abdn.ac.uk> Does anyone have tryed before Van Gieson staining on frozen sections? I'm working on 8-10 Microns sections, using vectabond slides. I've found lots of protocols on the net but all of them refer to wax embedded sections. I've also found different recipies for the Van Gieson Stain. Depending from the one you use, you could need to differentiate after stain with haematoxylin. what does it mean from the molecular point of view? -- La Manna Vincenzo PhD Student Department of Agriculture and Forestry, University of Aberdeen, Hilton Place block M AB24 4FA, Aberdeen , UK. Telephone; 01224 274259 Fax; 01224 273731 e-mail v.lamanna@abdn.ac.uk From haldana <@t> unimoron.edu.ar Fri Nov 14 12:27:43 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] LSAB or ABC the final question Message-ID: <00cb01c3aadd$008f6e00$7504a8c0@um.edu> Hi to the list. I need to buy some immunostaining system. We need to chose the ABC Elite or the LSAB+ (K067911). I work in rat tissue (cryostat or paraffine). Anybody have some advises for me please. We need to buy special rat serum to be applied with the universal antibody? Or may be obtained from the blood of one rat? thank in advance for all of you .... Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/dcbaa13b/attachment.htm From jlinda <@t> ces.clemson.edu Fri Nov 14 12:29:13 2003 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] What is Histology? Message-ID: <5.1.0.14.2.20031114131428.0305c630@mailhost.ces.clemson.edu> Dear Histology "Buddies", In honor of today being Friday, I want to share a funny with you...true story! It's definitely time we advertise our profession:-) A friend asked me where I had been recently and I replied that I had been attending the National Society for Histotechnology. They then wanted to know...what is histotechnology. I replied with the usual, "It's the study of tissue". He replied, "Oh, really...which kind...Puffs? or Kleenex??" And...he was serious! Have a great weekend! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From ccdub <@t> earthlink.net Fri Nov 14 13:51:16 2003 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Exhaust Block Message-ID: We have a pathologist who recently decided that if the specimen doesn't show a disease process (ie, no pathological abnormality) we must "exhaust the block". This means (to him) to make 3 additional levels (H&E's), then cut through the rest of the tissue leaving nothing in the block. We are suppose to do this to keep the patient or referring physician from requesting further testing on the block. So far we only have to do this with skin biopsies, but I am afraid he will soon want it for everything else. This is extremely time consuming! Do other labs out there do this? I am just curious. Cindy DuBois, HT ASCP Delta Pathology Assoc. Stockton, CA From yangpw <@t> umich.edu Fri Nov 14 14:01:38 2003 From: yangpw <@t> umich.edu (yangpw@umich.edu) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Immediate Early Gene expression in FISH Message-ID: <1068840098.3fb534a288cd3@carrierpigeon.mail.umich.edu> HI, I have been trying to do Fluorescent In situ for Immediate Early Gene (IEG such as c-fos, arc, etc.). I use riboprobe and tried bio-UTP/dig-UTP, rabbit anti-bio/dig, and then use fluorescein conjugated anti rabbit. I tried same protocol on enk mRNA and it turned out just fine. But IEG signals were always weak. Anything I can do to improve? Do I need to use any amplification kit like TSA? Any suggestion is welcome. Also I tried Alexa 350 for blue fluorescence, it seems photobleach very quick even with anti-fade media. Under microscope in couple minutes, it is fading. Does anyone have any experience on this? Which blue fluorescein is good? THanks a lot. Pengwei Yang, MD University of Michigan From CTague <@t> ahs.llumc.edu Fri Nov 14 14:12:18 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Exhaust Block Message-ID: i hope you get paid by the hour, that's rediculous! have fun, curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Cindy DuBois Sent: Friday, November 14, 2003 11:51 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exhaust Block We have a pathologist who recently decided that if the specimen doesn't show a disease process (ie, no pathological abnormality) we must "exhaust the block". This means (to him) to make 3 additional levels (H&E's), then cut through the rest of the tissue leaving nothing in the block. We are suppose to do this to keep the patient or referring physician from requesting further testing on the block. So far we only have to do this with skin biopsies, but I am afraid he will soon want it for everything else. This is extremely time consuming! Do other labs out there do this? I am just curious. Cindy DuBois, HT ASCP Delta Pathology Assoc. Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From ploykasek <@t> phenopath.com Fri Nov 14 15:47:31 2003 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] CSAII kit Message-ID: Just wondering if anyone out there in IHC land is using the CSAII detection kit from Dakocytomation? I am doing some trials with it and would like to discuss some techniques with anyone that has experience with this kit. Thanks for the help. Patti Loykasek Phenopath Laboratories Seattle, WA From gcallis <@t> montana.edu Fri Nov 14 15:48:08 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Exhaust Block In-Reply-To: Message-ID: <3.0.6.32.20031114144808.00bca888@gemini.msu.montana.edu> An interesting message on something I had never heard done before although we would often exhaust a block when it was postive. I am so curious as to why someone would need to do this?? Is it fear that if he made a mistake, it will never be detected or so he thinks? Is it to keep another pathologist from making money at his expense - after you did all the initial work? Is he afraid of criticism or disagreement about his diagnosis from someone else? What if an important test, say something in the molecular biology area is needed, even on that "negative" sample" - which your laboratory cannot perform, and you have no original sample left? I worked for a pathologist who alway maintained the tissue blocks and slides were the property of a patient and should be available for any consultation, at any time at request of attending physician or patient (who usually was going through their attending doc). Sometimes a patient had a difficult borderline diagnosis (not sure negative or positive) and copies of their reports plus original slides or recuts plus the original blocks were sent off with patient without question. What a shame! I hope he is never my pathologist! plus what a waste of time! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From Jackie.O'Connor <@t> abbott.com Fri Nov 14 16:08:24 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Exhaust Block Message-ID: This sounds, at the least, unscrupulous. I could see if he was asking to exhaust the block to ensure no pathology was missed in the entire sample - but to keep someone else from requesting additional work on the block??? If this is the case, it sounds like CYA in case he missed something - destroy the block so no one can prove him wrong, or sue him over a missed lesion? It's been known to happen. I've 'heard of' melanoma lesions appearing in deeper sections taken a few years after the initial diagnosis of "benign nevus". Never take your job for granted. Someone's life depends on your skills every single day. No pressure. Jackie O' "Tague, Curtis" Sent by: histonet-admin@lists.utsouthwestern.edu 11/14/2003 02:12 PM To: "Cindy DuBois" cc: "Histonet \(E-mail\)" Subject: RE: [Histonet] Exhaust Block i hope you get paid by the hour, that's rediculous! have fun, curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Cindy DuBois Sent: Friday, November 14, 2003 11:51 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exhaust Block We have a pathologist who recently decided that if the specimen doesn't show a disease process (ie, no pathological abnormality) we must "exhaust the block". This means (to him) to make 3 additional levels (H&E's), then cut through the rest of the tissue leaving nothing in the block. We are suppose to do this to keep the patient or referring physician from requesting further testing on the block. So far we only have to do this with skin biopsies, but I am afraid he will soon want it for everything else. This is extremely time consuming! Do other labs out there do this? I am just curious. Cindy DuBois, HT ASCP Delta Pathology Assoc. Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/62b34d4a/attachment.htm From p_bourne_14526 <@t> yahoo.com Fri Nov 14 16:44:06 2003 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] CSAII kit In-Reply-To: Message-ID: <20031114224406.97994.qmail@web10007.mail.yahoo.com> Great kit....just love it. --------------------------------- Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/1fe55170/attachment.htm From Tom_pella <@t> tedpella.com Fri Nov 14 20:09:11 2003 From: Tom_pella <@t> tedpella.com (Tom Pella) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] RE: Microwave Tissue Processors In-Reply-To: Message-ID: <006e01c3ab1d$766779c0$4420fdcc@domain> Sorry to jump in so late on this question. I have read the rest of the answers with interest. Here are a few things to consider: 1) FDA registration of a microwave unit is required for use in a histo lab in the USA; that is, the manufacturer needs to list their unit with the US FDA. To my knowledge, the only microwave tissue processor listed with the FDA is the PELCO HistoWave, also having been sold as the Thermo Shandon HistoWave. 2) The National Committee for Clinical Laboratory Standards ("NCCLS", which is now global and considering a name change to reflect that) has had a committee working on microwave standards in the histology lab for a long time now. I'm not sure, but I believe that this effort might be coming to a conclusion soon. I believe that several members of Histonet are on this committee. I'm not sure what kind of information is available on what it will be, but it is not yet in effect. Since NCCLS documents often are used to help create government standards, this will have an impact on histo labs that use microwaves for tissue processing. 3) The CAP Today article from October mentioned in another related email (I believe it was the one on microwave processing) describes a unit that is not yet for sale, but points to a direction that I believe will eventually take the histology tissue processing process by storm; that is, automated tissue processors that use a microwave. All of today's automated processors simply automate bench processes without accelerating them, which is good but leaves lots of room for speed improvements. Microwaves uniquely offer that possibility of speed improvement. A good microwave tissue processor, completely manual, can already run circles around the turnaround time of any of today's automated processors. I believe it's only a matter of time when all vendors who produce automated processors will come out with new models that incorporate a microwave in them; as the CAP article indicates, the time savings are a quantum leap over today's units. 4) Regarding Julee Chan's request on decal, we offer a unit that avoids the stronger corrosive acids like formic, rather using EDTA, and can do decal quickly with it. Heat is eliminated (it can process at ambient temperature, down to 4 degrees C). I think you will achieve superior staining results when you keep temperatures in the microwave low for both fixation (except for a brief time after diffusion) and decal. With our unit, there is no need to "let samples sit" for any length of time in formalin prior to fixation or EDTA prior to microwave decal. 5) I couldn't agree more with the comments on use of a lab microwave v. a kitchen microwave. There have been discussions before on Histonet about safety considerations, considerations of consistent processing results, equipment features, etc. that should persuade anyone who is serious about getting good results safely to only use a lab microwave. But I think there are easier ways to do antigen retrievel than in a microwave, though it's certainly possible. Tom Pella -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Hallada, Teri Sent: Tuesday, October 28, 2003 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Microwave Tissue Processors I was wondering if anyone out there is using a microwave tissue processor for routine hospital tissues. Are there any regulations applicable to instituting one, ie FDA approval? Teri Hallada BS MT CT (ASCP) thallada@noch.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- A non-text attachment was scrubbed... Name: winmail.dat Type: application/ms-tnef Size: 3592 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/ba919ea8/winmail.bin From Anita.QUIGLEY <@t> svhm.org.au Fri Nov 14 23:09:45 2003 From: Anita.QUIGLEY <@t> svhm.org.au (QUIGLEY Anita F (SVHM)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Connexin immunos Message-ID: Does anyone have an effective protocol for Connexin 43 and 32 immunohistochemistry on paraffin embedded brain or heart? Dr Anita Quigley, Melbourne Neuromuscular Research Institute, St. Vincent's Hospital, Fitzroy, 3065, Victoria, Australia. Ph: 61 3 92883341, Fax: 61 3 9288 3350 Anita.QUIGLEY@svhm.org.au -------------- next part -------------- Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. From DDDeltour <@t> sig.med.navy.mil Fri Nov 14 23:49:54 2003 From: DDDeltour <@t> sig.med.navy.mil (Deltour, Douglas D.(HM2)) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Exhaust Block Message-ID: It sounds like he is doing the overkill CYA thing. Was he ever a Navy Pathologist? HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy LPO Histology Histology Technician DSN 624-4669 FAX 624-4680 COMM (From US) 011-39-095-56-4669 DISCLAIMER: Confidentiality Notice - This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. **** Informazione Confidenziale - Questa e-Mail, incluso eventuali allegati, contiene informazioni confidenziali intese unicamente alla persona/e a cui e' indirizzata. Se avete ricevuto per errore questo messaggio, cortesemente contattare immediatamente il mittente e cancellare la e-Mail. Grazie****. -----Original Message----- From: Cindy DuBois [mailto:ccdub@earthlink.net] Sent: Friday, November 14, 2003 8:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exhaust Block We have a pathologist who recently decided that if the specimen doesn't show a disease process (ie, no pathological abnormality) we must "exhaust the block". This means (to him) to make 3 additional levels (H&E's), then cut through the rest of the tissue leaving nothing in the block. We are suppose to do this to keep the patient or referring physician from requesting further testing on the block. So far we only have to do this with skin biopsies, but I am afraid he will soon want it for everything else. This is extremely time consuming! Do other labs out there do this? I am just curious. Cindy DuBois, HT ASCP Delta Pathology Assoc. Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From punyasloke <@t> yahoo.com Sat Nov 15 10:10:13 2003 From: punyasloke <@t> yahoo.com (Punyasloke Bhadury) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] DNA extraction from formalin fixed samples Message-ID: <20031115161013.43618.qmail@web13806.mail.yahoo.com> Hello Everybody, Can anybody let me know how can I extract DNA from formalin fixed samples?Please let me know about the protocols. Thanks in advance. Punyasloke __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From gareth.davis <@t> Vanderbilt.Edu Sat Nov 15 15:28:48 2003 From: gareth.davis <@t> Vanderbilt.Edu (Davis, Gareth) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Perfusion pump time Message-ID: <082C721AF78DB34983E8BA2CD08546216A4105@mailbe07> Hello Histonetters, Recently (within the last couple months) I saw someone post perfusion times for pumps based on the animal being perfused. I tried to find the posting on the Histosearch, but unsuccessful. We are perfusing rats for the olfactory tissue, but would like ideals on the what others have done. Any input would be appreciated, including if heparin is needed in saline, the correct perfusion pump time, and if recirculation is necessary. thanks, Gareth Ms. Gareth B. Davis Research Assistant II -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031115/60b14d64/attachment.htm From CrochiereSteve <@t> aol.com Sat Nov 15 20:42:25 2003 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Exhaust Block Message-ID: How about this one: Recut X 2 - as many levels per slide as possible, then melt the block down and flip the tissue, repeat step one. (four slides total) P.s. this is done on all colonoscopy specimens for adenomas. How's that for overkill? Steven M. Crochiere, HT (ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center 299 Carew St. Springfield, MA 01104 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031115/3f8b0233/attachment.htm From tigersnake <@t> ecybermind.net Sun Nov 16 01:58:29 2003 From: tigersnake <@t> ecybermind.net (Paul Howard Lockwood) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] What is Histology? Message-ID: <200311160455.hAG4tBp16057@ginsberg.ecybermind.net> To All, I just get asked if I do historical work. But, I've been waiting to hear that one. Sincerely, Paul Lockwood 11/14/03 10:29:13 AM, Linda Jenkins wrote: >Dear Histology "Buddies", > In honor of today being Friday, I want to share a funny with >you...true story! > It's definitely time we advertise our profession:-) > A friend asked me where I had been recently and I replied that I >had been attending the National Society for Histotechnology. They then >wanted to know...what is histotechnology. I replied with the usual, "It's >the study of tissue". He replied, "Oh, really...which kind...Puffs? or >Kleenex??" And...he was serious! > Have a great weekend! > Linda > >Linda Jenkins, HT >Clemson University >Dept. of Bioengineering >Clemson, SC 29634-0905 >864.656.5553 >http://www.ces.clemson.edu/bio/research/histo/histo.htm > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jackie.O'Connor <@t> abbott.com Sun Nov 16 08:04:02 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] What is Histology? Message-ID: Once, when working in a hospital lab, a nurse accidentally called my lab to request a blood draw - I told her "You want service, this is parts." >From then on, I told people I worked in the Parts Department of the hospital. Another favorite when people ask "what is histology?" - "Did you ever have your tonsils removed? Did you ever wonder what happened to them after they took them out?" Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Fax 847.938.3266 Paul Howard Lockwood Sent by: histonet-admin@lists.utsouthwestern.edu 11/16/2003 01:58 AM To: Linda Jenkins , histonet@lists.utsouthwestern.edu cc: Subject: Re: [Histonet] What is Histology? To All, I just get asked if I do historical work. But, I've been waiting to hear that one. Sincerely, Paul Lockwood 11/14/03 10:29:13 AM, Linda Jenkins wrote: >Dear Histology "Buddies", > In honor of today being Friday, I want to share a funny with >you...true story! > It's definitely time we advertise our profession:-) > A friend asked me where I had been recently and I replied that I >had been attending the National Society for Histotechnology. They then >wanted to know...what is histotechnology. I replied with the usual, "It's >the study of tissue". He replied, "Oh, really...which kind...Puffs? or >Kleenex??" And...he was serious! > Have a great weekend! > Linda > >Linda Jenkins, HT >Clemson University >Dept. of Bioengineering >Clemson, SC 29634-0905 >864.656.5553 >http://www.ces.clemson.edu/bio/research/histo/histo.htm > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031116/6c4bae4c/attachment.htm From SAULSAULX <@t> aol.com Sun Nov 16 08:11:21 2003 From: SAULSAULX <@t> aol.com (SAULSAULX@aol.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] What is Histology? Message-ID: <76.34de3317.2ce8df89@aol.com> I need a ten page research paper on the trichrome stain can anyone help me/ From pruegg <@t> colobio.com Sun Nov 16 11:59:35 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] collagen IV In-Reply-To: Message-ID: Collagen antibodies can be obtained from Developmental Studies Hybridoma Bank, Department of Biological Sciences, University of Iowa, 007 BBE, IowA City, IA 52242-1324 tele: 319-335-3826, fax 319-335-2077 email dshb@uiowa.edu Collagen VI cat. #M3F7, species specificity listed for human and rat, but I have personally tested it on rabbit, goat and sheep with success so I would not hesitate to test it on dog tissue. I use pepsin digestion as pretreatment with Labelled polymer detection system or LSAB+ detection. Patsy Ruegg -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Leclerc Jocelyne Sent: Friday, November 14, 2003 9:41 AM To: histonet@pathology.swmed.edu Subject: [Histonet] collagen IV Hi all, I am looking for antibodies to collagen IV that work in formalin-fixed paraffin embedded DOG tissue. I would be very pleased if anyone could help me! Marianne Oswald Institute for Veterinary Anatomy University of Zurich, Switzerland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histosci <@t> shentel.net Sun Nov 16 11:57:11 2003 From: histosci <@t> shentel.net (HSRL) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] A marking pen that works! Message-ID: <001801c3ac6b$13f75400$0200a8c0@HSRLMAIN> Dear Netters, It is not often that vendors get praise from their clients via the histonet. But, I did want to take a minute to thank Statlab Medical Products for distributing a xylene-proof/alcohol-proof marking pen that works! After years of frustration using marking pens that stop working the day the cap is taken off, our time has come. We received our shipment of Statmark pens last week and are extatic with them. We bought ours from Statlab Medical Products 1-800-442-3573. Beth Poole HSRL- A GLP Compliant Laboratory 137 South Main Street Woodstock, Virginia 22664 (540)459-8211 Fax: (540)459-8217 beth@hsrl.org www.hsrl.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031116/ec4bb95f/attachment.htm From SAULSAULX <@t> aol.com Sun Nov 16 11:56:31 2003 From: SAULSAULX <@t> aol.com (SAULSAULX@aol.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] collagen IV Message-ID: <151.26dc54aa.2ce9144f@aol.com> I need a 10 page trichrome stain paper any help? From le.paix <@t> verizon.net Sun Nov 16 13:31:58 2003 From: le.paix <@t> verizon.net (Michael F.) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] what is histology In-Reply-To: <20031116180001.25112.46348.Mailman@swlx167.swmed.edu> References: <20031116180001.25112.46348.Mailman@swlx167.swmed.edu> Message-ID: as an aside, could you folks tell what percent would you say of HT/HTL start work before 5am? would you say that an HT/HTL would be more likely to have opportunities to work in research than a MT/MTL? I'm considering one or the other schools. Hope you don't mind my off topic posting. Please email me directly if its more appropriate. cheers, M. ...back to lurking From carl.hobbs <@t> kcl.ac.uk Sun Nov 16 13:52:55 2003 From: carl.hobbs <@t> kcl.ac.uk (Carl) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] re what is histology? References: <20031116180001.25112.46348.Mailman@swlx167.swmed.edu> Message-ID: <000601c3ac7b$46954b50$6bf78451@home> After a friend asked what I am/did, I expained about Histology, staining, dyes etc. I subsequently overheard him saying to another person that I was an artist! To him dyes/stains= paints...( hadn't listened to a word....or my explanation was inadequate lol) I suppose it's true; a picture paints a thousand words. Perhaps that's why, for example, the pathologist uses many words to describe what's seen in a section ;-) From cwscouten <@t> myneurolab.com Sun Nov 16 17:20:17 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Perfusion pump time Message-ID: I have responded separately with an attached 7 page discussion of perfusion, and a link to our Perfusion One product. Don't use flow rate, pressure is what the cardiovascular system controls, use 5% sucrose prewash, no saline, and switch to fixative when the animal stops. Haparin is not needed, all blood can be cleared without it with sufficient pressure. If any body else would like to see these materials, I will send them. Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: Davis, Gareth [mailto:gareth.davis@Vanderbilt.Edu] Sent: Saturday, November 15, 2003 3:29 PM To: Histonet Subject: [Histonet] Perfusion pump time Hello Histonetters, Recently (within the last couple months) I saw someone post perfusion times for pumps based on the animal being perfused. I tried to find the posting on the Histosearch, but unsuccessful. We are perfusing rats for the olfactory tissue, but would like ideals on the what others have done. Any input would be appreciated, including if heparin is needed in saline, the correct perfusion pump time, and if recirculation is necessary. thanks, Gareth Ms. Gareth B. Davis Research Assistant II -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031116/531dd53a/attachment.htm From i_stain <@t> yahoo.com Sun Nov 16 20:03:16 2003 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] e-cadherin antibody Message-ID: <20031117020316.51668.qmail@web42004.mail.yahoo.com> We are using monoclonal E-Cadherin in breast cancer tissues, clone: 4A2C7, from Zymed. Scott CSU From: "Nader, Alexander" To: "'histonet-admin@lists.utsouthwestern.edu'" Cc: "'Histonet@lists.utsouthwestern.edu'" Date: Wed, 12 Nov 2003 08:07:01 +0100 Subject: RE: [Histonet] e-cadherin antibody > Which clone are people using for e-caherin IHC on FFPE tissues? > Novocastra E-Cadherin clone 36B5 (IgG1) 1:50. Alexander Nader Inst. f. Pathology, Hanusch-Hospital Vienna, Austria __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From SAULSAULX <@t> aol.com Sun Nov 16 20:12:34 2003 From: SAULSAULX <@t> aol.com (SAULSAULX@aol.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] e-cadherin antibody Message-ID: <1a5.1c1895a5.2ce98892@aol.com> Histotechies......! I need help.where can i get a ten page double space research paper on the following two stains: 1.Alcian Blue 2.Trichrome stain thanks -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031116/1645ab9d/attachment.htm From azdudley <@t> hotmail.com Sun Nov 16 20:13:03 2003 From: azdudley <@t> hotmail.com (anita dudley) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation Message-ID: diana, I use penfix on my processor and we have good luck with our ers and prs, we also use it sometime to fix breast tissue before going on the processor. haven't had any problems with it. hope this helps. anita dudley providence hosp mobile alabama >From: "Goodwin, Diana" >To: "Histonet (E-mail)" >Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation >Date: Fri, 14 Nov 2003 11:39:21 -0500 > > > > Greetings, Histonetters. > > > > One of my pathologists has expressed concern that antigenicity for >certain > > antibodies, specifically ER and PR, in breast tissue that is processed > > using alcohol-formalin will be decreased. Can anyone cite a reference >in > > the literature that confirms or addresses this concern? Has anyone had > > this experience, or does anyone know of any reason that using > > alcohol-formalin (specifically Pen-Fix) would adversely affect IHC > > staining? > > > > Thanks to all of you, > > > > Diana Goodwin > > Supervisor, Anatomic Pathology > > Preston 6 > > 215-829-6532 > > e-mail: goodwind@pahosp.com > > _________________________________________________________________ Frustrated with dial-up? Get high-speed for as low as $26.95. https://broadband.msn.com (Prices may vary by service area.) From SAULSAULX <@t> aol.com Sun Nov 16 20:15:04 2003 From: SAULSAULX <@t> aol.com (SAULSAULX@aol.com) Date: Fri Sep 16 15:22:12 2005 Subject: [Histonet] e-cadherin antibody Message-ID: <28.40452994.2ce98928@aol.com> In a message dated 11/16/2003 9:14:01 PM Eastern Standard Time, SAULSAULX@aol.com writes: > Histotechies......! I need help.where can i get a ten page double space > research paper on the following two stains: > > 1.Alcian Blue > > 2.Trichrome stain > > > thanks Histotechies......! I need help.where can i get a ten page double space research paper on the following two stains: 1.Alcian Blue 2.Trichrome stain thanks -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031116/d8023dba/attachment.htm From jkiernan <@t> uwo.ca Sun Nov 16 23:47:47 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes (was Re:What is Histology?) References: <76.34de3317.2ce8df89@aol.com> Message-ID: <3FB86103.B523E25D@uwo.ca> The last 2 paragraphs of this email are not about trichrome staining. Are you (SAULSAULX@aol.com) looking for a review of postulated mechanisms of differential staining in the various trichrome methods, or for 10 pages of practical instructions, or for comparisons of different trichrome methods? If you let us know what you need you (and other Histonetters) will probably get several reading lists! The staining mechanisms are controversial because there has been too much speculation and not enough research. In this field you'll need to read 3 or 4 review articles to try to form a balanced view of your own. The mechanisms for trichromes (methods using 3 anionic dyes and PTA or PMA) need to be considered alongside methods using 2 anionic dyes in one solution (notably Van Gieson's stain and its less often used congeners). Is your name really SAULSAULX? It's much more satisfying to reply to a name at a real location such as, for example, Abey C. Defghi at the Royal Spithouse Infirmary in Badchester, Rayneshire, England. Histonet is a friendly community of over 1000 (I think; Herb or Linda might correct the number), and most of us indicate who we are when we ask or answer questions. On this note (and nothing to do with trichrome stains), I'd like to say how happy I was to see many Histonetters face to face at the recent NSH meeting in Louisville. I wish I could have talked with more of you, but it's easy to miss people in a big crowd. Also, the names on the lapel badges were printed too small. Such badges should be readable at a distance. Though not a shy person, I did not want to stare closely at the name label on every comely bosom. In these litiginous times everyone must avoid being greatly misunderstood. (NSH label makers please take note! Not many people have telescopic vision. Our instrument is the microscope.) -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ _______________________________________ SAULSAULX@aol.com wrote: > > I need a ten page research paper on the trichrome stain can anyone help me/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From i.lombaert <@t> med.rug.nl Mon Nov 17 01:39:57 2003 From: i.lombaert <@t> med.rug.nl (Lombaert) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] cytokeratin Message-ID: <200311170740.IAA11467@medmail.med.rug.nl> Hello everyone, Does anyone of you knows the existence of cytokeratin antibodies that are working on mouse parrafine tissues? Any help is welcome.. Thanks, Isabelle ----------------------- Ir. Isabelle Lombaert, MSc PhD student University of Groningen Faculty of Medical Sciences Antonius Deusinglaan 1 Building 3215, 5th floor, Room 553 9713 AV Groningen The Netherlands e-mail: I.Lombaert@med.rug.nl Tel.: +31 (0)50 363 29 15 From louise_renton <@t> hotmail.com Mon Nov 17 03:58:56 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Exhaust Block Message-ID: How about a simple mathematical calculation....measure the thickness of the embedded tissue and then estimate how many sections that will produce. Then present this to your pathologist, it might help to convince him of the waste of time and resources. I have cut over 400 sections from an average sized block of tissue. QED Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: Gayle Callis To: Cindy DuBois , Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Exhaust Block Date: Fri, 14 Nov 2003 14:48:08 -0700 An interesting message on something I had never heard done before although we would often exhaust a block when it was postive. I am so curious as to why someone would need to do this?? Is it fear that if he made a mistake, it will never be detected or so he thinks? Is it to keep another pathologist from making money at his expense - after you did all the initial work? Is he afraid of criticism or disagreement about his diagnosis from someone else? What if an important test, say something in the molecular biology area is needed, even on that "negative" sample" - which your laboratory cannot perform, and you have no original sample left? I worked for a pathologist who alway maintained the tissue blocks and slides were the property of a patient and should be available for any consultation, at any time at request of attending physician or patient (who usually was going through their attending doc). Sometimes a patient had a difficult borderline diagnosis (not sure negative or positive) and copies of their reports plus original slides or recuts plus the original blocks were sent off with patient without question. What a shame! I hope he is never my pathologist! plus what a waste of time! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Into gaming? Challenge online buddies to the game of your choice! http://messenger.msn.co.za/Resource/Games.aspx From abright <@t> brightinstruments.com Mon Nov 17 04:20:40 2003 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] thick frozen sections Message-ID: -Dear Cora, Your results indicate that the mouse brain is too cold, to rectify section @ -8 to -12?C and cool the knife at least 10? colder, if a specimen temperature control is not fitted achieve this with solid CO2 in contact with the knife. We achieve sections up to 600 microns using this method with ease. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Corazon D. Bucana [mailto:bucana@audumla.mdacc.tmc.edu] Sent: 13 November 2003 20:29 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thick frozen sections I would appreciate any suggestions on cutting 100 micron frozen sections of mouse brain for immunohistochemistry. our sections either crack or show a lot of chatter. Thanks, Cora Bucana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAULSAULX <@t> aol.com Mon Nov 17 05:57:17 2003 From: SAULSAULX <@t> aol.com (SAULSAULX@aol.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes (was Re:What is Histology?) Message-ID: <12d.34c81f46.2cea119d@aol.com> Ok guys i am sorry i was not clear initially on my request.Here is what I need,I am looking for a research paper type in which compares the different types of staining procedures and there uses.The two stains of interests are the Trichrome and the Alcian Blue.The paper must include the history of how the stain was introduced and the mechanism by which it works and lastly, for what purposes is the stain used.Idont know how anyone can write a 10 page paper on such,but that is the assignment I have and do not know how to proceed with this.Thanks Saul -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/689ff050/attachment.htm From rfail <@t> toolkitmail.com Mon Nov 17 06:36:54 2003 From: rfail <@t> toolkitmail.com (rfail@toolkitmail.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes (was Re:What is Histology?) Message-ID: <01L34PHKJR0896VXDD@SMTP00.InfoAve.Net> A little background research should give you the information you need to complete this assignment. Try the internet for the history.The book Mauve". a makes reference to a number of dyes. A large number of textbooks and journal articles can help you understand the mechanism of the stains. Rena Fail > Ok guys i am sorry i was not clear initially on my request.Here is what I > need,I am looking for a research paper type in which compares the different types > of staining procedures and there uses.The two stains of interests are the > Trichrome and the Alcian Blue.The paper must include the history of how the stain > was introduced and the mechanism by which it works and lastly, for what > purposes is the stain used.Idont know how anyone can write a 10 page paper on > such,but that is the assignment I have and do not know how to proceed with > this.Thanks > > > Saul > From ian.montgomery <@t> bio.gla.ac.uk Mon Nov 17 06:47:28 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:13 2005 Subject: Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?) Message-ID: <6.0.0.22.2.20031117124501.02d1a7b0@udcf.gla.ac.uk> Saul, Can I recommend you obtain a copy of Histological & Histochemical Methods (Theory and Practice) by J.A. Kiernan. It's full of the referenced information you require. Ian. >From: SAULSAULX@aol.com >To: jkiernan@uwo.ca >CC: Jackie.O'Connor@abbott.com, tigersnake@ecybermind.net, > histonet@lists.utsouthwestern.edu >X-Mailer: 8.0 for Windows sub 6024 >X-Scan-Signature: 690e9200c080ca55fd7a8d0fb0193eeb >Sender: histonet-admin@lists.utsouthwestern.edu >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.0.13 >List-Help: >List-Post: >List-Subscribe: , > >List-Id: For the exchange of information pertaining to histotechnology and >related fields >List-Unsubscribe: , > > >List-Archive: >Date: Mon, 17 Nov 2003 06:57:17 EST >X-Scan-Signature: 18b8f45f5f122038853a7119e027f2bd >X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu >Subject: Re: [Histonet] Trichromes (was Re:What is Histology?) >X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on > swlx167.swmed.edu >X-Spam-Status: No, hits=0.3 required=6.5 tests=HTML_MESSAGE,NO_REAL_NAME > autolearn=no version=2.60 >X-Spam-Level: >X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) >X-SA-Exim-Scanned: Yes >X-GLA-Spam-Filter: yes >X-GLA-Spam-Score: 0.7 (/) >X-GLA-Spam-Report: 0.3 NO_REAL_NAME From: does not include a >real name > 0.2 HTML_MESSAGE BODY: HTML included in message > 0.2 MIME_QP_LONG_LINE RAW: Quoted-printable line longer than > 76 chars > >Ok guys i am sorry i was not clear initially on my request.Here is what I >need,I am looking for a research paper type in which compares the >different types of staining procedures and there uses.The two stains of >interests are the Trichrome and the Alcian Blue.The paper must include the >history of how the stain was introduced and the mechanism by which it >works and lastly, for what purposes is the stain used.Idont know how >anyone can write a 10 page paper on such,but that is the assignment I have >and do not know how to proceed with this.Thanks > > >Saul Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/c524cfa8/attachment.htm From dgnajarian <@t> comcast.net Mon Nov 17 06:51:58 2003 From: dgnajarian <@t> comcast.net (Dennis Najarian) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] cytokeratin References: <200311170740.IAA11467@medmail.med.rug.nl> Message-ID: <001001c3ad09$9664b880$6501a8c0@natm.corp.chem> Chemicon International has Cytokeratin 5, 8, 10, & 18 for use on mouse paraffin tissues. They also have several others that have not been tested in-house, but they offer a 50% evaluation discount program. Dennis Najarian Technical Sales Representative Chemicon International ----- Original Message ----- From: "Lombaert" To: Sent: Sunday, November 16, 2003 11:39 PM Subject: [Histonet] cytokeratin > Hello everyone, > > Does anyone of you knows the existence of cytokeratin antibodies > that are working on mouse parrafine tissues? > Any help is welcome.. > > Thanks, > Isabelle > > > ----------------------- > Ir. Isabelle Lombaert, MSc > PhD student > University of Groningen > Faculty of Medical Sciences > Antonius Deusinglaan 1 > Building 3215, 5th floor, Room 553 > 9713 AV Groningen > The Netherlands > e-mail: I.Lombaert@med.rug.nl > Tel.: +31 (0)50 363 29 15 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Mon Nov 17 07:26:26 2003 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes (was Re:What is Histology?) References: <12d.34c81f46.2cea119d@aol.com> Message-ID: <003101c3ad0e$677d7b10$6400a8c0@bryanmainbox> Saul, Your difficulty is going to be keeping the paper to only 10 pages on each of the stains! The textile dye, Alcian blue 8Gx was formulated by Haddoch and Wood in 1944 and introduced as a histological stain by Steedman in 1950. Alcian blue can be used, either alone or in polychromatic mixtures, for a variety of staining methods. These range from the demonstration of amyloid to assorted glycosaminoglycans. As pointed out by John Kiernan, the trichrome methods are very numerous and varied in their application. You probably are most interested in those methods used for the demonstration of supporting(connective) tissue elements. Most likely the first of these was the single solution containing two anionic dyes introduced by Van Gieson in 1889. Today many people use the Masson (1929) technique in which two anionic dyes are applied sequentially, but separated by a heteropolyacid such as PTA. The first of many triacid stains was probably that of Mallory in 1900. I would recommend that you obtain the following texts from the library; The History of Staining, 3rd ed.,Clark and Kasten,Willams and Wilkins. ISBN 0-683-01705-5, Histopathological Technique and Practical Histochemistry, 3rd ed., R D Lillie, McGraw-Hill. Library of Congress Catalog Card Number: 64-7866 Histological and Histochemical Methods, 3rd ed., J.A.Kiernan, Butterworth Heinemann. ISBN 0 7506 4936 4 Good luck, Bryan ----- Original Message ----- From: SAULSAULX@aol.com To: jkiernan@uwo.ca Cc: Jackie.O'Connor@abbott.com ; tigersnake@ecybermind.net ; histonet@lists.utsouthwestern.edu Sent: Monday, November 17, 2003 6:57 AM Subject: Re: [Histonet] Trichromes (was Re:What is Histology?) Ok guys i am sorry i was not clear initially on my request.Here is what I need,I am looking for a research paper type in which compares the different types of staining procedures and there uses.The two stains of interests are the Trichrome and the Alcian Blue.The paper must include the history of how the stain was introduced and the mechanism by which it works and lastly, for what purposes is the stain used.Idont know how anyone can write a 10 page paper on such,but that is the assignment I have and do not know how to proceed with this.Thanks Saul -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/b865fe5b/attachment.htm From leanneharris <@t> eircom.net Mon Nov 17 08:09:42 2003 From: leanneharris <@t> eircom.net (leanneharris@eircom.net) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Hansson's Carbnic Anhydrase. Message-ID: Dear all, I wonder can you help me? I am looking for the working protocol for Hansson's Carbonic Anhydrase as I do not have access to the early edition of Pearse's Histochemistry. Could anybody please forward me a copy of the protocol? Thnaking you in advance, Leanne. Leanne Harris, Dublin Institute of Technology, School of Biological Sciences, Kevin Street, Dublin 8. Ireland. 00-353-1-4022888 leanneharris@eircom.net From GDawson <@t> Milw.Dynacare.com Mon Nov 17 07:55:55 2003 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] What is histology? Message-ID: My Lab's canned answer to the question of what a histotechnologist does is: "We deal with bits and pieces of humanity" ;) Happy Monday, Glen Dawson From cfavara <@t> niaid.nih.gov Mon Nov 17 08:26:55 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies Message-ID: Primary antibody: purified rabbit anti-beta-Galactosidase [with no cross reactivity with E. coli]. Source: Cappell, ICN Biochemical Division 1263 S. Chillocothe Rd Aurora, Ohio 44202 1-800-279-5490 Cat# 55976 Lot# 40337 In my hands works well with just about any detection 1:1000 or greater dilution. Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: t-kuzniar@md.northwestern.edu [mailto:t-kuzniar@md.northwestern.edu] Sent: Tuesday, November 11, 2003 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti-beta-galactosidase antibodies Has anyone had any experience with polyclonal antibody against b-galactosidase? What reporter system have you used? Thank you. Tom Kuzniar _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronnie_Houston <@t> bshsi.com Mon Nov 17 08:43:50 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Hansson's Carbnic Anhydrase. Message-ID: <530361BF03351B4CAE5270A05D3037B5FE0535@bsrexms01.BSHSIR.COM> Leanne, Without knowing in what context you are wanting to use Hanson's technique, I would recommend finding a suitable antibody and doing IHC. >From my recollection, the original method of Hanson uses free-floating frozen sections, and the sections do not withstand the procedure very well. In addition, consistency is a major problem. The enzyme seems to tolerate acetone fixation fairly well; aldehyde fixation is only successful in highly active sites. There is a gel-method, and a semi-permeable membrane method that are superior if you must go the route of enzyme histochemistry. Let me know if you need this info. Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com -----Original Message----- From: leanneharris@eircom.net [mailto:leanneharris@eircom.net] Sent: Monday, November 17, 2003 9:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hansson's Carbnic Anhydrase. Dear all, I wonder can you help me? I am looking for the working protocol for Hansson's Carbonic Anhydrase as I do not have access to the early edition of Pearse's Histochemistry. Could anybody please forward me a copy of the protocol? Thnaking you in advance, Leanne. Leanne Harris, Dublin Institute of Technology, School of Biological Sciences, Kevin Street, Dublin 8. Ireland. 00-353-1-4022888 leanneharris@eircom.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From tpschaer <@t> vet.upenn.edu Mon Nov 17 08:51:09 2003 From: tpschaer <@t> vet.upenn.edu (Tom Schaer) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] polymerization failure _ Technovit 9100New Message-ID: <034701c3ad1a$3cc84a60$38595b82@vet.upenn.edu> Dear all: We are having some problems with the polymerization reaction of Technovit 9100New. Despite keeping things at constant temperatures it fails to polymerize. We are polymerizing specimens of ovine fem cond with approx 100ml of constituted solution in glass jars. We have trial vials at 4C, room temp., and -18C and none polymerize. We used the manufacturer's protocol included in the 9100New kit. Thanks for any insight and help. Best, Tom -------------------------------------------- Thomas P. Schaer, VMD Comparative Orthopaedic Research & Tissue Engineering Department of Clinical Studies University of Pennsylvania New Bolton Center 382 West Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office) tel. 610-444-5800 (x6131 lab) fax. 610-925-8100 tpschaer@mail.vet.upenn.edu -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/e604f936/attachment.htm From haldana <@t> unimoron.edu.ar Mon Nov 17 09:37:14 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes References: <6.0.0.22.2.20031117124501.02d1a7b0@udcf.gla.ac.uk> Message-ID: <004401c3ad20$acbf9020$7504a8c0@um.edu> Saul Two papers are wonderful in relation to the biological staining. The fisrt "A contribution to the theory of biological staining based on the principles of structural organization of biological macromolecules " Biotechnic and Histochemistry, 2001, 76(3): 137-161. This explain the posible form of binding to collagen in trichormes and other staining formulas. Is a complex paper but is superb. The second "Van Gieson?s picrofuchsin. The staining mechanisms for collaegen and cytoplasm, and examination of the dye diffusion rate model of differential staining". Histochemistry (1993). 99: 163-174 is related of the staining mechansims of van Gieson?s stain technique. Both papers were made by Poul Prento, I contact him several times for some dudes related with staining methos and he always answer me in a perfect form. Do it, write to him the mail is prento@mail.tele.dk Sorry for my bad English Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: Ian Montgomery To: histonet@lists.utsouthwestern.edu Sent: Monday, November 17, 2003 9:47 AM Subject: Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?) Saul, Can I recommend you obtain a copy of Histological & Histochemical Methods (Theory and Practice) by J.A. Kiernan. It's full of the referenced information you require. Ian. From: SAULSAULX@aol.com To: jkiernan@uwo.ca CC: Jackie.O'Connor@abbott.com, tigersnake@ecybermind.net, histonet@lists.utsouthwestern.edu X-Mailer: 8.0 for Windows sub 6024 X-Scan-Signature: 690e9200c080ca55fd7a8d0fb0193eeb Sender: histonet-admin@lists.utsouthwestern.edu X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.0.13 List-Help: List-Post: List-Subscribe: , List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: Date: Mon, 17 Nov 2003 06:57:17 EST X-Scan-Signature: 18b8f45f5f122038853a7119e027f2bd X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichromes (was Re:What is Histology?) X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on swlx167.swmed.edu X-Spam-Status: No, hits=0.3 required=6.5 tests=HTML_MESSAGE,NO_REAL_NAME autolearn=no version=2.60 X-Spam-Level: X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) X-SA-Exim-Scanned: Yes X-GLA-Spam-Filter: yes X-GLA-Spam-Score: 0.7 (/) X-GLA-Spam-Report: 0.3 NO_REAL_NAME From: does not include a real name 0.2 HTML_MESSAGE BODY: HTML included in message 0.2 MIME_QP_LONG_LINE RAW: Quoted-printable line longer than 76 chars Ok guys i am sorry i was not clear initially on my request.Here is what I need,I am looking for a research paper type in which compares the different types of staining procedures and there uses.The two stains of interests are the Trichrome and the Alcian Blue.The paper must include the history of how the stain was introduced and the mechanism by which it works and lastly, for what purposes is the stain used.Idont know how anyone can write a 10 page paper on such,but that is the assignment I have and do not know how to proceed with this.Thanks Saul Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/22fec230/attachment.htm From alex.knisely <@t> kcl.ac.uk Mon Nov 17 10:19:34 2003 From: alex.knisely <@t> kcl.ac.uk (Alex Knisely) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes and "ten-page double-spaced paper" In-Reply-To: <004401c3ad20$acbf9020$7504a8c0@um.edu> References: <6.0.0.22.2.20031117124501.02d1a7b0@udcf.gla.ac.uk> Message-ID: <3.0.6.32.20031117161934.03f4be60@137.73.66.5> Am I the only curmudgeon reading these exchanges? I'm embarrassed for myself by the good will and helpfulness shown Saulsaulx by Listmembers. I'd taken it as given that (s)he wanted to find ten double-spaced pages on trichrome technique, WRITTEN BY SOMEONE ELSE, that (s)he could hand in, retyped, under her / his own name. And I'd made up my mind to remember that name, if it ever emerged on-list, so that I could be sure not to hire her / him if the occasion ever arose. But, when I see the outpouring of information that Listers have made available, it seems that I misjudged the situation -- with all this help, how could Saulsaulx NOT write a decent paper on his / her own? Alex K Alex Knisely, MD Consultant Histopathologist alex.knisely@kcl.ac.uk Institute of Liver Studies King's College Hospital Denmark Hill London SE5 9RS UK +44 (0)20 - 7346 - 3125 telefax +44 (0)20 - 7346 - 4627 office From mlm11 <@t> cornell.edu Mon Nov 17 10:59:33 2003 From: mlm11 <@t> cornell.edu (Mary Lou) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] trichrome paper Message-ID: <5.2.1.1.2.20031117114616.00bbe450@postoffice9.mail.cornell.edu> No, you're not the only curmudgeon (great word!). In the past literature searches have been time consuming and sometimes frustrating. These days, typing 'trichrome', etc, into PubMed should release a slew of papers. This person is relying on the good will of the Histonet to do all the footwork. Let us hope that the individuals here are properly acknowledged. On the other hand, maybe this person only has email and not internet access. Mary Lou ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Am I the only curmudgeon reading these exchanges? I'm embarrassed for myself by the good will and helpfulness shown Saulsaulx by Listmembers. I'd taken it as given that (s)he wanted to find ten double-spaced pages on trichrome technique, WRITTEN BY SOMEONE ELSE, that (s)he could hand in, retyped, under her / his own name. And I'd made up my mind to remember that name, if it ever emerged on-list, so that I could be sure not to hire her / him if the occasion ever arose. But, when I see the outpouring of information that Listers have made available, it seems that I misjudged the situation -- with all this help, how could Saulsaulx NOT write a decent paper on his / her own? Alex K Alex Knisely, MD Consultant Histopathologist alex.knisely@kcl.ac.uk Institute of Liver Studies King's College Hospital Denmark Hill London SE5 9RS UK +44 (0)20 - 7346 - 3125 telefax +44 (0)20 - 7346 - 4627 office _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/8aee35d7/attachment.htm From gcallis <@t> montana.edu Mon Nov 17 11:31:36 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes (was Re:What is Histology?) In-Reply-To: <12d.34c81f46.2cea119d@aol.com> Message-ID: <3.0.6.32.20031117103136.00bd3e00@gemini.msu.montana.edu> Access books on histotechnics or histotechnology, and dig into their reference lists, you will find it plus the books have good explanations also. Some books can be found in university libraries and histopathology laboratories. You should do a literature search and it is surprising what you will find on the internet. Hmmm 10 page review, yes - possible for these two stains - you have to dig it out as they stain some very different tissue components. At 06:57 AM 11/17/2003 EST, you wrote: >Ok guys i am sorry i was not clear initially on my request.Here is what I >need,I am looking for a research paper type in which compares the different >types of staining procedures and there uses.The two stains of interests are >the Trichrome and the Alcian Blue.The paper must include the history of how >the stain was introduced and the mechanism by which it works and lastly, >for what purposes is the stain used.Idont know how anyone can write a 10 >page paper on such,but that is the assignment I have and do not know how to >proceed with this.Thanks > > > Saul Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From settembr <@t> umdnj.edu Mon Nov 17 11:47:40 2003 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] CSAII kit Message-ID: I use the CSAII kit with my tough Cyclin D1. Best staining i've had so far. DakoCytomation helped me out a lot to get this to work. Dana Settembre >>> Patti Loykasek 11/14/2003 1:47:31 PM >>> Just wondering if anyone out there in IHC land is using the CSAII detection kit from Dakocytomation? I am doing some trials with it and would like to discuss some techniques with anyone that has experience with this kit. Thanks for the help. Patti Loykasek Phenopath Laboratories Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchung <@t> ppmh.org Mon Nov 17 11:36:29 2003 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] re what is histology? Message-ID: That is funny. I ran into the same situation about "Cytology". When someone asked me what do I do? I said "Cytology", then they said "Psychology"? I will explain to them "NO, not psychology, It's Cytology and it's study of CELL" In return they replied "so what do you SALE and do you work on a commission". -----Original Message----- From: Carl [mailto:carl.hobbs@kcl.ac.uk] Sent: Sunday, November 16, 2003 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re what is histology? After a friend asked what I am/did, I expained about Histology, staining, dyes etc. I subsequently overheard him saying to another person that I was an artist! To him dyes/stains= paints...( hadn't listened to a word....or my explanation was inadequate lol) I suppose it's true; a picture paints a thousand words. Perhaps that's why, for example, the pathologist uses many words to describe what's seen in a section ;-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. From marirose.satterfield <@t> MercyMemorial.org Mon Nov 17 12:16:09 2003 From: marirose.satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Statlab marking pens Message-ID: I agree with Beth. We received a sample of Statlab marking pens and cannot believe the difference between them and Secureline. We have officially switched. No more drying out YEAH! Marirose Satterfield Histology Supervisor From cfavara <@t> niaid.nih.gov Mon Nov 17 12:48:57 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Statlab marking pens Message-ID: I called this morning to get a sample can hardly wait to get them - Maybe a great Christmas present! Yeah! x Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Satterfield, Marirose [mailto:marirose.satterfield@MercyMemorial.org] Sent: Monday, November 17, 2003 11:16 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Statlab marking pens I agree with Beth. We received a sample of Statlab marking pens and cannot believe the difference between them and Secureline. We have officially switched. No more drying out YEAH! Marirose Satterfield Histology Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tvedilago <@t> system1.net Mon Nov 17 13:22:40 2003 From: tvedilago <@t> system1.net (Tommy Vedilago) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Career steps Message-ID: Hello Histonetters, We are actively searching for a Histology Supervisor for the Philadelphia area. The pay and benefits for this position are outstanding and the facility is located outside of the city itself. It is a very culturally active area with lots to offer for the active lifestyle. We need someone who is driven to excel regardless of the challenge and enjoys making a positive impact in a difficult job. We are also searching for a Manager in D.C, a supervisor in Maine, a Manager in NYC, a supervisor in Long Island, and a supervisor for a startup with an established reference lab in Houston, a supervisor in Phoenix, and tech positions in many other areas. The field of Histology is booming and there are few candidates for these jobs. This is the time to take the opportunity to further your career. I am here to help and please feel free to call me at 866-797-8361. Thanks and have a great day. Sincerely, Tommy Vedilago System 1 Search (864) 627-0012 (864) 627-0013 Fax From kab35 <@t> cornell.edu Mon Nov 17 13:48:49 2003 From: kab35 <@t> cornell.edu (Kathie Berghorn) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Help needed with Archives Message-ID: Dear Listers, Could someone please help me........I want to search the archives but when I go to the archive site on the web I can view messages by Date, subject, author or thread but have no clue on how to search. I would appreciate any instruction on how to search the archives. Kathie From ccdub <@t> earthlink.net Mon Nov 17 14:10:14 2003 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Exhaust Block Decision Message-ID: Thank you for all of your replies. I brought our lab manager in on the problem and it was decided in our lab that if a pathologist wants to cut completely thru the block, he must: 1) dictate the reason in his report 2) Have another pathologist in the group agree with him. Thanks for all of your input, it truly helped out lab make a decision. Cindy DuBois From Megan.Kear <@t> hunter.health.nsw.gov.au Mon Nov 17 14:23:24 2003 From: Megan.Kear <@t> hunter.health.nsw.gov.au (Megan Kear) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] biogenesis source Message-ID: Hi Histonetters I am looking for the company in Australia that sells Biogenesis products. thank you. Megan Kear Australia From pmarcum <@t> polysciences.com Mon Nov 17 14:30:46 2003 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Exhaust Block Decision In-Reply-To: Message-ID: <003a01c3ad49$ae64b800$4800a8c0@PMARCUM2K> Sounds like a good plan and makes him give you an explanation to be sure you are covered. Pam Marcum > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Cindy > DuBois > Sent: Monday, November 17, 2003 3:10 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Exhaust Block Decision > > > Thank you for all of your replies. I brought our lab manager in on the > problem and it was decided in our lab that if a pathologist wants to cut > completely thru the block, he must: > 1) dictate the reason in his report > 2) Have another pathologist in the group agree with him. > > Thanks for all of your input, it truly helped out lab make a decision. > > Cindy DuBois > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From asmith <@t> mail.barry.edu Mon Nov 17 14:47:09 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] re what is histology? Message-ID: <494304423C63E246A5CF87A3AEEB577011B5AC@bumail01.barrynet.barry.edu> Actually, we are artists. Much of the pleasure in our job comes from its aesthetic quality. One may need Gabe's or Masson's trichrome to tell collagen from smooth muscle in a blindworm (or other totally unfamiliar critter), but one uses trichromes on human tissue because the effect is beautiful. The real payoff in a Mallory AzAn stain is contemplating the beauty of the final product. Even a well-stained eosinophil or Paneth cell is beautiful. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: Carl [mailto:carl.hobbs@kcl.ac.uk] Sent: Sunday, November 16, 2003 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re what is histology? After a friend asked what I am/did, I expained about Histology, staining, dyes etc. I subsequently overheard him saying to another person that I was an artist! To him dyes/stains= paints...( hadn't listened to a word....or my explanation was inadequate lol) I suppose it's true; a picture paints a thousand words. Perhaps that's why, for example, the pathologist uses many words to describe what's seen in a section ;-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From hymclab <@t> hyhc.com Mon Nov 17 14:45:06 2003 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] A marking pen that works! Message-ID: <529F3A73499ED611AA9D00A0C9558E4E43EF11@hyhcexchange.hyhc.local> We love them too!!! Dawn Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI -----Original Message----- From: HSRL [mailto:histosci@shentel.net] Sent: Sunday, November 16, 2003 11:57 AM To: histonet@pathology.swmed.edu Subject: [Histonet] A marking pen that works! Dear Netters, It is not often that vendors get praise from their clients via the histonet. But, I did want to take a minute to thank Statlab Medical Products for distributing a xylene-proof/alcohol-proof marking pen that works! After years of frustration using marking pens that stop working the day the cap is taken off, our time has come. We received our shipment of Statmark pens last week and are extatic with them. We bought ours from Statlab Medical Products 1-800-442-3573. Beth Poole HSRL- A GLP Compliant Laboratory 137 South Main Street Woodstock, Virginia 22664 (540)459-8211 Fax: (540)459-8217 beth@hsrl.org www.hsrl.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/0b8e9ec4/attachment.htm From rperkinsfsu <@t> hotmail.com Mon Nov 17 15:42:43 2003 From: rperkinsfsu <@t> hotmail.com (Randa Perkins) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Perfusions of young mice Message-ID: To all: If anyone has any advice on perfusing very young mice I would be grateful to hear it. We are trying to harvest the brains of mice at P9 (post-natal day 9) and younger. I've been able to perfuse adult mice with the standard method of injecting preperfusion and paraformaldehyde solutions into the left ventricle, with a cut in the right atrium pre-injection. My biggest problem, as I recently tried perfusing a P9 mouse, is that the heart is so small that I have yet to successfully place the needle in the left ventricle and inject without going through and getting little to no perfusion. That and accessing the right atrium to cut it and allow outflow. The heart is quite small. Any suggestions? Thanks, Randa Perkins Randa M. Perkins Research Assistant Florida State University Tallahassee, FL _________________________________________________________________ >From Beethoven to the Rolling Stones, your favorite music is always playing on MSN Radio Plus. No ads, no talk. Trial month FREE! http://join.msn.com/?page=offers/premiumradio From cfavara <@t> niaid.nih.gov Mon Nov 17 16:53:46 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Perfusions of young mice Message-ID: Randa, I have done as young as P5. I agree they are very small. Things I found most helpful are: 1. good lighting some type of fiber optic is what I use 2. magnifying glasses - the kind that surgeons use some even have a light source 3. winged infusion needles 27Gx1/2" I used 3-5 ml syringes just found it easier and practice!practice!practice! good luck! Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Randa Perkins [mailto:rperkinsfsu@hotmail.com] Sent: Monday, November 17, 2003 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Perfusions of young mice To all: If anyone has any advice on perfusing very young mice I would be grateful to hear it. We are trying to harvest the brains of mice at P9 (post-natal day 9) and younger. I've been able to perfuse adult mice with the standard method of injecting preperfusion and paraformaldehyde solutions into the left ventricle, with a cut in the right atrium pre-injection. My biggest problem, as I recently tried perfusing a P9 mouse, is that the heart is so small that I have yet to successfully place the needle in the left ventricle and inject without going through and getting little to no perfusion. That and accessing the right atrium to cut it and allow outflow. The heart is quite small. Any suggestions? Thanks, Randa Perkins Randa M. Perkins Research Assistant Florida State University Tallahassee, FL _________________________________________________________________ >From Beethoven to the Rolling Stones, your favorite music is always playing on MSN Radio Plus. No ads, no talk. Trial month FREE! http://join.msn.com/?page=offers/premiumradio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From achertcoff <@t> velocom.com.ar Fri Nov 14 16:57:42 2003 From: achertcoff <@t> velocom.com.ar (Agustin V Chertcoff) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Trichromes References: <6.0.0.22.2.20031117124501.02d1a7b0@udcf.gla.ac.uk> <004401c3ad20$acbf9020$7504a8c0@um.edu> Message-ID: <00ba01c3ab03$7bdc69c0$c5c13bc8@pinagus> Don't worry Hernan! I understand your grammar perfectly! Ht. Agustin Victor Chertcoff Hospital Municipal de Gastroenterologia Servicio de Patologia 54-11-43064641 interno 144 Instituto Nacional de Microbiologia C.G.Malbran Departamento de Virologia Servicio de Microscopia Electronica 54-11-43017428 interno 26 Ciudad Autonoma de Buenos Aires-Argentina achertcoff@tutopia.com ----- Original Message ----- From: Hernan Aldana Marcos To: histonet@lists.utsouthwestern.edu Sent: Monday, November 17, 2003 12:37 PM Subject: Re: Re: [Histonet] Trichromes Saul Two papers are wonderful in relation to the biological staining. The fisrt "A contribution to the theory of biological staining based on the principles of structural organization of biological macromolecules " Biotechnic and Histochemistry, 2001, 76(3): 137-161. This explain the posible form of binding to collagen in trichormes and other staining formulas. Is a complex paper but is superb. The second "Van Gieson?s picrofuchsin. The staining mechanisms for collaegen and cytoplasm, and examination of the dye diffusion rate model of differential staining". Histochemistry (1993). 99: 163-174 is related of the staining mechansims of van Gieson?s stain technique. Both papers were made by Poul Prento, I contact him several times for some dudes related with staining methos and he always answer me in a perfect form. Do it, write to him the mail is prento@mail.tele.dk Sorry for my bad English Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: Ian Montgomery To: histonet@lists.utsouthwestern.edu Sent: Monday, November 17, 2003 9:47 AM Subject: Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?) Saul, Can I recommend you obtain a copy of Histological & Histochemical Methods (Theory and Practice) by J.A. Kiernan. It's full of the referenced information you require. Ian. From: SAULSAULX@aol.com To: jkiernan@uwo.ca CC: Jackie.O'Connor@abbott.com, tigersnake@ecybermind.net, histonet@lists.utsouthwestern.edu X-Mailer: 8.0 for Windows sub 6024 X-Scan-Signature: 690e9200c080ca55fd7a8d0fb0193eeb Sender: histonet-admin@lists.utsouthwestern.edu X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.0.13 List-Help: List-Post: List-Subscribe: , List-Id: For the exchange of information pertaining to histotechnology and related fields List-Unsubscribe: , List-Archive: Date: Mon, 17 Nov 2003 06:57:17 EST X-Scan-Signature: 18b8f45f5f122038853a7119e027f2bd X-SA-Exim-Mail-From: histonet-admin@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichromes (was Re:What is Histology?) X-Spam-Checker-Version: SpamAssassin 2.60 (1.212-2003-09-23-exp) on swlx167.swmed.edu X-Spam-Status: No, hits=0.3 required=6.5 tests=HTML_MESSAGE,NO_REAL_NAME autolearn=no version=2.60 X-Spam-Level: X-SA-Exim-Version: 3.1 (built Tue Sep 9 12:31:04 CDT 2003) X-SA-Exim-Scanned: Yes X-GLA-Spam-Filter: yes X-GLA-Spam-Score: 0.7 (/) X-GLA-Spam-Report: 0.3 NO_REAL_NAME From: does not include a real name 0.2 HTML_MESSAGE BODY: HTML included in message 0.2 MIME_QP_LONG_LINE RAW: Quoted-printable line longer than 76 chars Ok guys i am sorry i was not clear initially on my request.Here is what I need,I am looking for a research paper type in which compares the different types of staining procedures and there uses.The two stains of interests are the Trichrome and the Alcian Blue.The paper must include the history of how the stain was introduced and the mechanism by which it works and lastly, for what purposes is the stain used.Idont know how anyone can write a 10 page paper on such,but that is the assignment I have and do not know how to proceed with this.Thanks Saul Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031114/0f698264/attachment.htm From georgecole <@t> ev1.net Mon Nov 17 20:18:49 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Plastic molds for freezing tissues and STATIC CHARGE CONTROL Message-ID: <000101c3ad7a$4fe8d460$0a4dbad0@hppav> Histotechs: The molds that work very well with muscle and nerve and just about everything else are from TISSUE TEC. They are called CRYOMOLDS. The standard size is 25mm by 20mm by 5mm. The intermediate size is 15mm by 15mm by 5mm. They are thin walled plastic boats that work just great with OCT and they can be reused if handled carefully. For the control of STATIC CHARGES ON TISSUES ---- The STATICMASTER IONIZING UNIT MODEL NO. 2U5OO The address of the supplier: 2937 ALT BLVD. GRAND ISLAND N.Y. 14072. I used them for 40 years. They have a flexible shaft holder for the unit that makes it easy to direct the deionization. You can order two If you are really bothered by static cling---like in a very dry climate---order two of the 2U500's and tape them together on the stand. There is a 1000 unit, but that takes a radioactive substance permit. I used 2 units taped together in rainy Oregon, and I think all of you would prefer the double set up. The emitters are polonium and emit particles that do not pierce the skin, They warn you not to eat the strips under the grid. I don't see how you COULD eat what's under the grid. Sometimes I wish someone would do it, so I could find out how the heck he did it. georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/5f660f8e/attachment.htm From georgecole <@t> ev1.net Tue Nov 18 00:27:45 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Section thickness in immunofluorecsence studies Message-ID: <000001c3ad9d$15c21ee0$054dbad0@hppav> HISTOTECHS DOING IMMUNOFLUORESCENCE SSUDIES. I just visited the hospital I retired from. In talking with the great gal who replaced me and my good friend who runs the immuno lab I mentioned that when I did immunofluorescence studies on kidney, I cut sections thicker than anyone else---I was shaky with 2 or 3 micron sections as a beginner, so I cut one 3 micron, then went up to 5 microns, and, because I had been doing nothing but muscles and nerves in which I did 8 and 10 micron sections, I included an 8 micron section. Well the kidney turned out to fluoresce with two preps. The 3 micron section was not very dramatic. The 8 micron section was brilliant, but a bit globby; but to my surprise, all hands chose the 6 micron section. Also, as I had developed the multiple sections techniques on muscles and nerves, I cut one 3 micron section for slide 1, 2 and 3, then went back and cut a 6 micron section for slide 1, 2 and 3, and did the same for 8 microns sections. Well, I never went back to the 2 or 3 micron sections. I made 6 microns standard----and got many beautiful photos brilliantly shining. The 8 micron section was a little much, so I used that only with bigger pieces of tissue. But remember what we are doing in this procedure----we are NOT staining fine nuclear detail that takes thin sections to do justice to---we are staining BLOBS and the photos of the 6 microns sections use to be passed around getting OOOHHED and AAAHHHED by the residents. Also, about the multiple sections---this wasn't always possible, because the kidney biopsies are usually quite small and I had to leave tissue enough to do the Jones silvers----but in this case, of the three sections on one positive prep, only one of the sections lit up. And what did that tell me? It told me that a single section per slide work up on this case could miss being positive something like 66% of the time. So I kept doing a 3 micron and as many 6 micron sections cut for each slide as the size of the tissue would allow. The 3 micron section NEVER was positive, by chance or by whatever----and I passed around many brilliantly lighted kidney sections photos of the 6 micron sections. georgecole@ev1.net . -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031117/4169d9e6/attachment.htm From Inga.Hansson <@t> neuro.uu.se Tue Nov 18 01:30:56 2003 From: Inga.Hansson <@t> neuro.uu.se (Inga Hansson) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] caspase-3 antibody Message-ID: Hi all! I bet there?s someone out there that knows of an antibody that can detect active caspase-3 in mouse (frozen or paraffin sections) Thanks in advance! Inga -- Inga Hansson Dept. neuroscience, div. neurobiology PO Box 587, BMC Husargatan 3 S-751 23 Uppsala SWEDEN Phone: +46(18) 471 4384 Fax: +46(18)559017 From n.benabdallah <@t> anatom.unizh.ch Tue Nov 18 04:27:46 2003 From: n.benabdallah <@t> anatom.unizh.ch (Nada Ben Abdallah) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] (no subject) Message-ID: <3.0.6.32.20031118112746.0122e100@rzu-mailhost.unizh.ch> Dear all, I am a freshly starting PhD student and my project concerns mainly dealing with mouse brain frozen sections. My first work was just to practice histology was to cut 40 microns coronal sections and nissl stain them. Everything worked fine except that the sections started to fold when stained (although I mounted them on a gelatine coated slides and used gelatine to fix them more on the slides). PS: the mouse from which the brain was taken was perfused with this procedure: -PBS -sulfid -parafolmaldehyde+picric acid and then the brain was placed in 30%sucrose for long time until used. I appreciate it if you have any suggestions before I proceed with my work. Best wishes, nada From Barry.R.Rittman <@t> uth.tmc.edu Tue Nov 18 06:19:10 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Section thickness in immunofluorecsence studies Message-ID: <566FB0B522443D43AF02D2ADBE35A6F06358A7@UTHEVS3.mail.uthouston.edu> George you have hit upon a common problem. It seems that the tendency has been "the thinner the better". I believe that for histology, there is a need for both thin sections and thick sections. Thinner sections can provide superior reolution but sometimes we lose the wood for the trees. It should be remembered that counting cell numbers in thinner sections is fraught with disaster, the thinner the section the greater the error. Abercrombie discused this a long time ago. I would urge y'all to repeat George's experience and also try thicker sections and see the difference. Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu on behalf of George Cole Sent: Tue 11/18/2003 12:27 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Section thickness in immunofluorecsence studies HISTOTECHS DOING IMMUNOFLUORESCENCE SSUDIES. I just visited the hospital I retired from. In talking with the great gal who replaced me and my good friend who runs the immuno lab I mentioned that when I did immunofluorescence studies on kidney, I cut sections thicker than anyone else---I was shaky with 2 or 3 micron sections as a beginner, so I cut one 3 micron, then went up to 5 microns, and, because I had been doing nothing but muscles and nerves in which I did 8 and 10 micron sections, I included an 8 micron section. Well the kidney turned out to fluoresce with two preps. The 3 micron section was not very dramatic. The 8 micron section was brilliant, but a bit globby; but to my surprise, all hands chose the 6 micron section. Also, as I had developed the multiple sections techniques on muscles and nerves, I cut one 3 micron section for slide 1, 2 and 3, then went back and cut a 6 micron section for slide 1, 2 and 3, and did the same for 8 microns sections. Well, I never went back to the 2 or 3 micron sections. I made 6 microns standard----and got many beautiful photos brilliantly shining. The 8 micron section was a little much, so I used that only with bigger pieces of tissue. But remember what we are doing in this procedure----we are NOT staining fine nuclear detail that takes thin sections to do justice to---we are staining BLOBS and the photos of the 6 microns sections use to be passed around getting OOOHHED and AAAHHHED by the residents. Also, about the multiple sections---this wasn?t always possible, because the kidney biopsies are usually quite small and I had to leave tissue enough to do the Jones silvers----but in this case, of the three sections on one positive prep, only one of the sections lit up. And what did that tell me? It told me that a single section per slide work up on this case could miss being positive something like 66% of the time. So I kept doing a 3 micron and as many 6 micron sections cut for each slide as the size of the tissue would allow. The 3 micron section NEVER was positive, by chance or by whatever----and I passed around many brilliantly lighted kidney sections photos of the 6 micron sections. georgecole@ev1.net . From StarkusL <@t> ummhc.org Tue Nov 18 06:51:20 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Help again with mouse gastroenterology slides Message-ID: A couple weeks ago, I asked a question about getting the folds out of the slides I was making of mouse stomach. Several people replied and told me about using ammonium hydroxide. I have pretty much removed all of the folding. However, another problem still remains. My Post-doc says that some of the pieces are damaged. Attached are pictures of this damage. For some reason, the stain looks funny as well but that is from taking the pictures. When you look at it under a microscope, the H+E looks fine. Any suggestions are welcome as I am at a complete loss. <<405 x100.jpg>> <<471 x100.jpg>> -------------- next part -------------- A non-text attachment was scrubbed... Name: 405 x100.jpg Type: image/jpeg Size: 12930 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/2921c933/405x100.jpg -------------- next part -------------- A non-text attachment was scrubbed... Name: 471 x100.jpg Type: image/jpeg Size: 13362 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/2921c933/471x100.jpg From Nancy.Walker <@t> sanofi-synthelabo.com Tue Nov 18 07:06:46 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] =?iso-8859-1?Q?R=E9f=2E_=3A_[Histonet]_Perfusions_of_young_mice?= Message-ID: We've done up to P6 mice. We use a "syringe pushing" pump. Use two syringes (one with PBS and one with PFA) hooked up to thin tubing and a two way valve, more tubing then an adapter that fits on a 30 gage needle, with the needle part fitting into the thin catheter (this is hard to explain), we added a thin flexible needle (but it's not sharp, sorry I don't know where we got it or what they are used for, I'll try and find out more) onto the end of the catheter. We pierce the ventricle with the needle and hold it there , cut the right atrium wash for 30" with PBS then 5 ' of PFA 4% ( flow rate of 0.25ml/min). If you have a hard time seeing what your doing use a magnifying glass like some people use for a microtome, or a dissecting microscope. hope this helps...good luck, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From WArnold546 <@t> aol.com Tue Nov 18 07:40:58 2003 From: WArnold546 <@t> aol.com (WArnold546@aol.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Fwd: Fw: Weel What Have You Got To Loose Message-ID: <151.26f3f89d.2ceb7b6a@aol.com> Skipped content of type multipart/alternative-------------- next part -------------- An embedded message was scrubbed... From: "jose trevino" Subject: Fwd: Fw: Weel What Have You Got To Loose Date: Mon, 17 Nov 2003 15:04:49 -0600 Size: 8409 Url: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/ed9cccaa/attachment.eml From Sw918890 <@t> aol.com Tue Nov 18 07:43:35 2003 From: Sw918890 <@t> aol.com (Sw918890@aol.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Golgi-Cox Method Message-ID: <7C8114EB.6BA2E730.0082A251@aol.com> I was wondering if anyone out there had a staining protocol for Golgi-Cox. My principle investigator is having me investigate kits for this stain. We have found a source, but I also want to see other protocols out there as well. Thanks in advance, Steven R. Westra University of Iowa Health Care (319) 356-1090 From Jackie.O'Connor <@t> abbott.com Tue Nov 18 07:50:28 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Fwd: Fw: Weel What Have You Got To Loose Message-ID: My valuable time. Don't ever send this junk again. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics WArnold546@aol.com Sent by: histonet-admin@lists.utsouthwestern.edu 11/18/2003 07:40 AM To: tim@krgv.com, jfrederick@imaginail.com, histonet@pathology.swmed.edu cc: Subject: [Histonet] Fwd: Fw: Weel What Have You Got To Loose ----- Message from on ----- Keep it going what have you got to loose... >From: Agk9nat@aol.com >To: Kdgk9@aol.com, tbabyjj@hotmail.com, jag_9311@yahoo.com >Subject: Fwd: Fw: Weel What Have You Got To Loose >Date: Sun, 16 Nov 2003 16:49:03 EST > > Send a QuickGreet with MSN Messenger. ----- Message from on ----- ----- Original Message ----- From: "Nancy Toler" To: ; "Sandy" ; ; ; ; ; ; ; ; ; ; ; ; ; ; Sent: Friday, November 14, 2003 7:03 PM Subject: Fwd: Weel What Have You Got To Loose > What if this is real? It's sort of like the lottery. With my luck, the > deadline was yesterday. > > > To all of my friends, I do not usually forward > messages, but this is from my good friend Pearlas > Sanborn and she really is an attorney. > > If she says that this will work - it WILL work. After > all, what have you got to lose? > > SORRY EVERYBODY..JUST HAD TO TAKE THE CHANCE!!! > I'm an attorney, and I know the law. This thing is for > real. > > Rest assured AOL and Intel will follow through with > their promises for fear of facing a > multimillion-dollar class action suit similar to the > one filed by PepsiCo against General Electric not too > long ago. > > Dear Friends, > > Please do not take this for a junk letter. Bill Gates > is sharing his fortune. If you ignore this you will > repent later. Microsoft and AOL are now the largest > Internet companies and in an effort to make sure that > Internet Explorer remains the most widely used > program, Microsoft and AOL are running an e-mail beta > test. > > When you forward this e-mail to friends, Microsoft can > and will track it if you are a Microsoft Windows user) > for a two week time period. > > For every person that you forward this e-mail to, > Microsoft will pay you $245.00, for every person that > you sent it to that forwards it on, Microsoft will pay > you $243.00 and for every third person that receives > it, you will be paid $241.00. Within two weeks, > Microsoft will contact you for your address and then > send you a cheque. > > Regards. > > Charles S. Bailey > General Manager Field Operations > 1-800-842-2332 Ext. 1085 or 904-245-1085 or RNX > 292-1085 > Charles_Bailey@csx.com > > I thought this was a scam myself, but two weeks after > receiving this e-mail and forwarding it on, Microsoft > contacted me for my address and within days, I > received a cheque for US$24,800.00. You need to > respond before the beta testing is over. If anyone can > afford this Bill Gates is the man. > > It's all marketing expense to him. Please forward this > to as many people as possible. You are bound to get at > least US$10,000.00. We're not going to help them out > with their e-mail beta test without getting a little > something for our time. My brother's girlfriend got in > on this a few months ago. When I went to visit him for > the Baylor/UT game. > > She showed me her check. It was for the sum of > $4,324.44 and was stamped "Paid In Full". Like I said > before, I know the law, and this is for real Intel and > AOL are now discussing a merger which would make them > the largest Internet company and in an effort make > sure that AOL remains the most widely used program, > Intel and AOL are running an e-mail beta test. > > When you forward this e-mail to friends, Intel can and > will track it (if you are a Microsoft Windows user) > for a two week time period. For every person that you > forward this e-mail to, Microsoft will pay you > $203.15. > > For every person that you sent it to that forwards it > on, Microsoft will pay you $156.29 And for every third > person that receives it, you will be paid $17.65. > Within two weeks, Intel will contact you for your > address and then send you a check. > > I thought this was a scam myself, but a friend of my > good friend's Aunt Patricia, who works at Intel, > actually got a check of $4,54323 by forwarding this > e-mail. > > Try it; what have you got to lose???? > > > __________________________________ > Do you Yahoo!? > Protect your identity with Yahoo! Mail AddressGuard > http://antispam.yahoo.com/whatsnewfree > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/582520f4/attachment.htm From Jackie.O'Connor <@t> abbott.com Tue Nov 18 07:47:14 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] caspase-3 antibody Message-ID: BD Pharmingen, Purified Rabbit Anti-Active Caspase-3 Monoclonal Antibody Catalog #559565 - I use it for murine tissues every day, SABC protocol. One hour incubation - however, my paraffin sections are zinc fixed - not formalin. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Inga Hansson Sent by: histonet-admin@lists.utsouthwestern.edu 11/18/2003 01:30 AM To: histonet@pathology.swmed.edu cc: Subject: [Histonet] caspase-3 antibody Hi all! I bet there?s someone out there that knows of an antibody that can detect active caspase-3 in mouse (frozen or paraffin sections) Thanks in advance! Inga -- Inga Hansson Dept. neuroscience, div. neurobiology PO Box 587, BMC Husargatan 3 S-751 23 Uppsala SWEDEN Phone: +46(18) 471 4384 Fax: +46(18)559017 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/9065ec07/attachment.htm From juan.gutierrez <@t> christushealth.org Tue Nov 18 08:27:43 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] e-cadherin antibody Message-ID: You write it. -----Original Message----- From: SAULSAULX@aol.com [mailto:SAULSAULX@aol.com] Sent: Sun 11/16/2003 8:15 PM To: SAULSAULX@aol.com; i_stain@yahoo.com; alexander.nader@wgkk.sozvers.at Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] e-cadherin antibody In a message dated 11/16/2003 9:14:01 PM Eastern Standard Time, SAULSAULX@aol.com writes: Histotechies......! I need help.where can i get a ten page double space research paper on the following two stains: 1.Alcian Blue 2.Trichrome stain thanks Histotechies......! I need help.where can i get a ten page double space research paper on the following two stains: 1.Alcian Blue 2.Trichrome stain thanks From jlf <@t> jax.org Tue Nov 18 08:42:21 2003 From: jlf <@t> jax.org (Judi Ford) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Eosinophil stains for mouse tissue Message-ID: <5.1.0.14.0.20031118094002.00a6b2e8@aretha.jax.org> Hi everyone, We are looking for a stain which will emphasize the eosinophils in mouse heart and lung tissue. Any help is greatly appreciated. Thanks, Judi Ford Jackson Laboratory Bar Harbor, ME From kerney <@t> fas.harvard.edu Tue Nov 18 09:04:25 2003 From: kerney <@t> fas.harvard.edu (Ryan Kerney) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Triton vs. Tween Message-ID: <1069167865.3fba34f990c4d@webmail.fas.harvard.edu> Can anyone out there tell me when you are supposed to use tritonX instead of tween 20 and why some protocols use both? Are they both just mild detergents? What is the difference? thanks, Ryan -- Ryan Kerney Department of Herpetology Museum of Comparative Zoology 26 Oxford St. Cambridge, MA 02138 Office: 617-496-4065 Lab: 617-496-4632 From Luis.Chiriboga <@t> med.nyu.edu Tue Nov 18 09:42:12 2003 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] caspase-3 antibody In-Reply-To: Message-ID: Cell signaling Cat #9661 rabbit polyclonal detects active Caspase 3 works in Fresh & FFPE human and mouse tissues -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com Sent: Tuesday, November 18, 2003 8:47 AM To: Inga Hansson Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] caspase-3 antibody BD Pharmingen, Purified Rabbit Anti-Active Caspase-3 Monoclonal Antibody Catalog #559565 - I use it for murine tissues every day, SABC protocol. One hour incubation - however, my paraffin sections are zinc fixed - not formalin. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Inga Hansson Sent by: histonet-admin@lists.utsouthwestern.edu 11/18/2003 01:30 AM To: histonet@pathology.swmed.edu cc: Subject: [Histonet] caspase-3 antibody Hi all! I bet there?s someone out there that knows of an antibody that can detect active caspase-3 in mouse (frozen or paraffin sections) Thanks in advance! Inga -- Inga Hansson Dept. neuroscience, div. neurobiology PO Box 587, BMC Husargatan 3 S-751 23 Uppsala SWEDEN Phone: +46(18) 471 4384 Fax: +46(18)559017 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/61cda27e/attachment.htm From ian.montgomery <@t> bio.gla.ac.uk Tue Nov 18 09:49:27 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:13 2005 Subject: Fwd: [Histonet] Eosinophil stains for mouse tissue Message-ID: <6.0.0.22.2.20031118153352.02d157f0@udcf.gla.ac.uk> Judi, Recently this has been the bane of my life, eosinophils in mouse tissue. Name it and I've tried it. Carbol chromotrope, lovely on other species. Various combinations of eosin, strong, weak, long staining, short staining. Luna's eosinophil granule stain, biebrich scarlet. For me, the best technique by far for mouse tissue is, Sirius Red (Llewellyn,B.D. 1970. J. Med. Technol. 27. 308-309). Works a treat every time. All it needs is a light Haematoxylin and you have lovely stain. I would try immuno but I'm having a bit of bother finding an antibody, anybody suggest a source? Ian. >Hi everyone, > >We are looking for a stain which will emphasize the eosinophils in mouse >heart and lung tissue. Any help is greatly appreciated. > >Thanks, > >Judi Ford >Jackson Laboratory >Bar Harbor, ME > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/369ef34b/attachment.htm From jkiernan <@t> uwo.ca Tue Nov 18 10:02:49 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Golgi-Cox Method References: <7C8114EB.6BA2E730.0082A251@aol.com> Message-ID: <3FBA42A9.1BE766DC@uwo.ca> The variant of Gibb & Kolb (1998) J. Neurosci. Methods 79:1-4 gives excellent impregnation of dendrites and their spines. The use of a vibrating microtome to cut the thick sections is an improvement over the traditional nitrocellulose embedding or using frozen sections. With any Golgi-Cox procedure you'll be using large amounts of mercuric chloride, so be careful. The used solution cannot be sent down the drain. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ______________________________________ Sw918890@aol.com wrote: > > I was wondering if anyone out there had a staining protocol for Golgi-Cox. My principle investigator is having me investigate kits for this stain. We have found a source, but I also want to see other protocols out there as well. > > Thanks in advance, > > Steven R. Westra > University of Iowa Health Care > (319) 356-1090 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Tue Nov 18 10:09:38 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Triton vs. Tween Message-ID: I use TritonX to permeablize cells that have been cultured for staining. Tween I use as a wetting agent in buffers when washing slides this is what makes the wash buffer sheet off the slide. I think?!@ c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Ryan Kerney [mailto:kerney@fas.harvard.edu] Sent: Tuesday, November 18, 2003 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Triton vs. Tween Can anyone out there tell me when you are supposed to use tritonX instead of tween 20 and why some protocols use both? Are they both just mild detergents? What is the difference? thanks, Ryan -- Ryan Kerney Department of Herpetology Museum of Comparative Zoology 26 Oxford St. Cambridge, MA 02138 Office: 617-496-4065 Lab: 617-496-4632 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> FAIRVIEW.ORG Tue Nov 18 10:35:05 2003 From: JSCHUMA1 <@t> FAIRVIEW.ORG (JENNIFER SCHUMACHER) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Nile Red Message-ID: Has anyone heard of a nile red stain? The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/9de75f50/attachment.htm From making <@t> nersp.nerdc.ufl.edu Tue Nov 18 10:49:49 2003 From: making <@t> nersp.nerdc.ufl.edu (Mike King) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Statlab marking pens Message-ID: <3FBA4DAD.9020501@nersp.nerdc.ufl.edu> Are these the Sakura Pigma pens as seen on the Statlab web page? (http://www.statlab.com/product.asp?catalog%5Fname=StatLab&category%5Fname=Histology+and+Cytology+Specialties&product%5Fid=SL660) >From: "Satterfield, Marirose" >To: "'histonet@lists.utsouthwestern.edu'" > >Date: Mon, 17 Nov 2003 13:16:09 -0500 >Subject: [Histonet] Statlab marking pens >I agree with Beth. We received a sample of Statlab marking pens and >cannot >believe the difference between them and Secureline. We have officially >switched. No more drying out YEAH! >Marirose Satterfield >Histology Supervisor From Rcartun <@t> harthosp.org Tue Nov 18 10:57:14 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Endogenous enzyme activity - eosinophils Message-ID: Do eosinophils have a different type of endogenous peroxidase that survives quenching in 3% hydrogen peroxide prior to immunohistochemical staining? Thanks! I have seen a few cases recently where the eosinophils are strongly reactive following normal quenching. Interestingly, pretreatment with antigen retrieval using heat destroys this reactivity. Richard Cartun From georgecole <@t> ev1.net Tue Nov 18 11:00:06 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Mary and Don on the thickness of immuno sections Message-ID: <000001c3adf5$6bea8430$034dbad0@hppav> Mary and Don, see the two entries in today's histonet on section thickness for immuno slides. georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/c3fa3f9b/attachment.htm From mcdonal1 <@t> ccf.org Tue Nov 18 11:01:58 2003 From: mcdonal1 <@t> ccf.org (Linda McDonald) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Non-disposable microtome knives Message-ID: Is anyone interested in the old microtome knives? We have a some and want to get rid of them. Let me know. Thnaks, Linda McDonald ------------------------------------------------------------------------------ Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ============================================================================== From JSCHUMA1 <@t> FAIRVIEW.ORG Tue Nov 18 11:07:02 2003 From: JSCHUMA1 <@t> FAIRVIEW.ORG (JENNIFER SCHUMACHER) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Nile Red Message-ID: I am sorry histonetters . . . I checked the archives AFTER sending my original request . . . I have found plenty of information and references. Please disregard the earlier question. Jen The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/8e8f74b0/attachment.htm From jkiernan <@t> uwo.ca Tue Nov 18 11:45:19 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Nile Red References: Message-ID: <3FBA5AAF.AB2AAA18@uwo.ca> JSCHUMA1@FAIRVIEW.ORG wrote: > Has anyone heard of a nile red stain? > Yes. It's the oxidation product of nile blue. You can make your own or buy it. It's used as a stain for lipids or as a fluorescent probe for hydrophobic sites (in proteins, as well as lipids). Solid cholesterol crystals pick up enough nile red to fluoresce but they are not made visible in ordinary light microscopy. In Cain's nile blue method, nile red is responsible for the red or pink coloration of hydrophobic lipids; free fatty acids and phospholipids are stained blue by the unoxidized nile blue. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From tpmorken <@t> labvision.com Tue Nov 18 12:02:32 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Triton vs. Tween Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD27@usca0082k03.rallansci.apogent.com> Ryan, while both are non-ionic surfactants/detergents, Tween 20 is milder than than Triton X100. Tween is used extensively in detergents of all sorts (even those you buy in the store) and in our field is used as a spreading agent and blocking agent for immunochemistry. Triton is used primarily for protein extraction and in whole cells or tissue sections for membrane permeablization. An excellent booklet on detergents for biology is available online from CalBioChem at: http://www.emdbiosciences.com/SharedImages/TechnicalLiterature/1_Detbooklet_ 2001.pdf Tim Morken Lab Vision / NeoMarkers www.labvision.com -----Original Message----- From: Ryan Kerney [mailto:kerney@fas.harvard.edu] Sent: Tuesday, November 18, 2003 7:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Triton vs. Tween Can anyone out there tell me when you are supposed to use tritonX instead of tween 20 and why some protocols use both? Are they both just mild detergents? What is the difference? thanks, Ryan -- Ryan Kerney Department of Herpetology Museum of Comparative Zoology 26 Oxford St. Cambridge, MA 02138 Office: 617-496-4065 Lab: 617-496-4632 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From n.benabdallah <@t> anatom.unizh.ch Tue Nov 18 12:51:33 2003 From: n.benabdallah <@t> anatom.unizh.ch (Nada Ben Abdallah) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Re: answers on folded sections In-Reply-To: <4285bb428d64.428d644285bb@shaw.ca> Message-ID: <3.0.6.32.20031118195133.01227230@rzu-mailhost.unizh.ch> Dear colleagues, thank you for your replies. to answer your question hernan, this perfusion protocol is used for both Timm and neurogenesis. that's why I used it with my mice. As for the drying of the slides, I already left the slides ON at room temperature but as you suggested stephanie I will leave them longer and maybe try at 40?C. Hope this will work. Sincerely, nada At 08:49 18.11.03 -0800, slieblich@shaw.ca wrote: >I have also done 40um sections and nissl. My suggestion is to let them dry at least over night (up to a couple of days) before staining. Further, you can put them in a humidifier (or water bath, but just above the water surface)for 10-20 mins and let them really stick on. That's all I can think of, or maybe use subbed slides.... >Hope this helps! > >Stephanie Lieblich >University of British Columbia > >----- Original Message ----- >From: Nada Ben Abdallah >Date: Tuesday, November 18, 2003 2:27 am >Subject: [Histonet] (no subject) > >> >> >> Dear all, >> I am a freshly starting PhD student and my project concerns mainly >> dealingwith mouse brain frozen sections. My first work was just to >> practicehistology was to cut 40 microns coronal sections and nissl >> stain them. >> Everything worked fine except that the sections started to fold when >> stained (although I mounted them on a gelatine coated slides and used >> gelatine to fix them more on the slides). >> PS: the mouse from which the brain was taken was perfused with >> this procedure: >> -PBS >> -sulfid >> -parafolmaldehyde+picric acid >> and then the brain was placed in 30%sucrose for long time until used. >> >> I appreciate it if you have any suggestions before I proceed with >> my work. >> >> Best wishes, >> nada >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From Janet.Bonner <@t> FLHOSP.ORG Tue Nov 18 13:00:48 2003 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Fwd: Fw: Weel What Have You Got To Loose Message-ID: <9F4410664F56DC4A8B2BD42920756D0C490C86@fh2k093.fhmis.net> But you just sent it anyway to - how many people?! --Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Tuesday, November 18, 2003 8:50 AM To: WArnold546@aol.com Cc: histonet@pathology.swmed.edu; histonet-admin@lists.utsouthwestern.edu; jfrederick@imaginail.com; tim@krgv.com Subject: Re: [Histonet] Fwd: Fw: Weel What Have You Got To Loose My valuable time. Don't ever send this junk again. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics WArnold546@aol.com Sent by: histonet-admin@lists.utsouthwestern.edu 11/18/2003 07:40 AM To: tim@krgv.com, jfrederick@imaginail.com, histonet@pathology.swmed.edu cc: Subject: [Histonet] Fwd: Fw: Weel What Have You Got To Loose ----- Message from on ----- Keep it going what have you got to loose... >From: Agk9nat@aol.com >To: Kdgk9@aol.com, tbabyjj@hotmail.com, jag_9311@yahoo.com >Subject: Fwd: Fw: Weel What Have You Got To Loose >Date: Sun, 16 Nov 2003 16:49:03 EST > > _____ Send a QuickGreet with MSN Messenger. ----- Message from on ----- ----- Original Message ----- From: "Nancy Toler" To: ; "Sandy" ; ; ; ; ; ; ; ; ; ; ; ; ; ; Sent: Friday, November 14, 2003 7:03 PM Subject: Fwd: Weel What Have You Got To Loose > What if this is real? It's sort of like the lottery. With my luck, the > deadline was yesterday. > > > To all of my friends, I do not usually forward > messages, but this is from my good friend Pearlas > Sanborn and she really is an attorney. > > If she says that this will work - it WILL work. After > all, what have you got to lose? > > SORRY EVERYBODY..JUST HAD TO TAKE THE CHANCE!!! > I'm an attorney, and I know the law. This thing is for > real. > > Rest assured AOL and Intel will follow through with > their promises for fear of facing a > multimillion-dollar class action suit similar to the > one filed by PepsiCo against General Electric not too > long ago. > > Dear Friends, > > Please do not take this for a junk letter. Bill Gates > is sharing his fortune. If you ignore this you will > repent later. Microsoft and AOL are now the largest > Internet companies and in an effort to make sure that > Internet Explorer remains the most widely used > program, Microsoft and AOL are running an e-mail beta > test. > > When you forward this e-mail to friends, Microsoft can > and will track it if you are a Microsoft Windows user) > for a two week time period. > > For every person that you forward this e-mail to, > Microsoft will pay you $245.00, for every person that > you sent it to that forwards it on, Microsoft will pay > you $243.00 and for every third person that receives > it, you will be paid $241.00. Within two weeks, > Microsoft will contact you for your address and then > send you a cheque. > > Regards. > > Charles S. Bailey > General Manager Field Operations > 1-800-842-2332 Ext. 1085 or 904-245-1085 or RNX > 292-1085 > Charles_Bailey@csx.com > > I thought this was a scam myself, but two weeks after > receiving this e-mail and forwarding it on, Microsoft > contacted me for my address and within days, I > received a cheque for US$24,800.00. You need to > respond before the beta testing is over. If anyone can > afford this Bill Gates is the man. > > It's all marketing expense to him. Please forward this > to as many people as possible. You are bound to get at > least US$10,000.00. We're not going to help them out > with their e-mail beta test without getting a little > something for our time. My brother's girlfriend got in > on this a few months ago. When I went to visit him for > the Baylor/UT game. > > She showed me her check. It was for the sum of > $4,324.44 and was stamped "Paid In Full". Like I said > before, I know the law, and this is for real Intel and > AOL are now discussing a merger which would make them > the largest Internet company and in an effort make > sure that AOL remains the most widely used program, > Intel and AOL are running an e-mail beta test. > > When you forward this e-mail to friends, Intel can and > will track it (if you are a Microsoft Windows user) > for a two week time period. For every person that you > forward this e-mail to, Microsoft will pay you > $203.15. > > For every person that you sent it to that forwards it > on, Microsoft will pay you $156.29 And for every third > person that receives it, you will be paid $17.65. > Within two weeks, Intel will contact you for your > address and then send you a check. > > I thought this was a scam myself, but a friend of my > good friend's Aunt Patricia, who works at Intel, > actually got a check of $4,54323 by forwarding this > e-mail. > > Try it; what have you got to lose???? > > > __________________________________ > Do you Yahoo!? > Protect your identity with Yahoo! Mail AddressGuard > http://antispam.yahoo.com/whatsnewfree > The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From marirose.satterfield <@t> MercyMemorial.org Tue Nov 18 13:04:37 2003 From: marirose.satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Statlab pens Message-ID: I guess I should have read all my new Histonet e-mail before I responded to the first one that I opened. We have been using the Statlab pen on our tissue cassettes and on our slides. They have not faded during any of the process. In a side by side comparison you just couldn't believe the difference in the amount of fading by the Secureline. Believe me I was very skeptical when the sales rep told me that people were just raving about these pens but after trying them our lab loves them. Maybe this will push Secureline into improving their pens that we have been putting up with for years just because that's all that was out there. Call Statlab 1-800-442-3573 catalog number SMP-BK Marirose Satterfield Histology Supervisor From Jackie.O'Connor <@t> abbott.com Tue Nov 18 13:09:59 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Fwd: Fw: Weel What Have You Got To Loose Message-ID: Unintentionally, but Touche'. Then YOU sent it again . . . . But you just sent it anyway to - how many people?! --Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Tuesday, November 18, 2003 8:50 AM To: WArnold546@aol.com Cc: histonet@pathology.swmed.edu; histonet-admin@lists.utsouthwestern.edu; jfrederick@imaginail.com; tim@krgv.com Subject: Re: [Histonet] Fwd: Fw: Weel What Have You Got To Loose My valuable time. Don't ever send this junk again. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/e1eabde8/attachment.htm From scoop <@t> mail.nih.gov Tue Nov 18 13:58:09 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] mouse blastocyst staining Message-ID: Thanks to everyone for the advice on NADPH Diaphorase. Now, does anyone have experience staining mouse blastocysts? Do you react with antibodies before or after immobilizing it on the slide? How do you immobilize it on the slide? Is there anything that will permeabilize the membranes or do you have to use an early age? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From hborgeri <@t> wfubmc.edu Tue Nov 18 14:29:44 2003 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Unsubscribe Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8E97B@EXCHVS2.medctr.ad.wfubmc.edu> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 3781 bytes Desc: image001.jpg Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/1c360e55/attachment.jpg -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 15492 bytes Desc: image002.gif Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/1c360e55/attachment.gif From GDawson <@t> Milw.Dynacare.com Tue Nov 18 14:38:40 2003 From: GDawson <@t> Milw.Dynacare.com (Dawson, Glen) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] RE: What have you got to lose? Message-ID: What have I got to lose? In Summary: 1)My Patience 2)My Mind 3)My faith in my fellow man 4)My temper 5)My respect for anyone who actually still falls for a scam that is so old that it has Stegosaurus feces on it. Sincerely, Glen Dawson From StarkusL <@t> ummhc.org Tue Nov 18 14:47:54 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] RE: What have you got to lose? Message-ID: Well said, Glen! People, this information is available on the internet. WWW.Snopes.com tells you whether these emails are urban legends or not! I'm sick of getting these stupid emails. -----Original Message----- From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com] Sent: Tuesday, November 18, 2003 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: What have you got to lose? What have I got to lose? In Summary: 1)My Patience 2)My Mind 3)My faith in my fellow man 4)My temper 5)My respect for anyone who actually still falls for a scam that is so old that it has Stegosaurus feces on it. Sincerely, Glen Dawson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tracey.couse <@t> ibb.gatech.edu Tue Nov 18 15:53:10 2003 From: tracey.couse <@t> ibb.gatech.edu (Tracey Couse) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Book Search Message-ID: <5.0.0.25.2.20031118152333.049fbae0@mail.ibb.gatech.edu> Hello to all, I just received notification from Amazon, that John Alan Kiernan's "Histological and Histochemical Methods: Theory and Practice" book is not available from any of their sources. Does anyone know from where I can purchase this? If not, can someone suggest an appropriate equivalent? I am interested in a book that will detail the chemical nature of dyes as well as their staining mechanisms...basically an extension of his talk at this year's NSH meeting. I am familiar with the "Best of the Histonet book list", but I am not sure of the best substitution(s). As always, thanks for any help. Tracey Couse Laboratory Coordinator/Histologist Georgia Tech/Emory Center for the Engineering of Living Tissues Georgia Institute of Technology From Histologia <@t> aol.com Tue Nov 18 14:57:54 2003 From: Histologia <@t> aol.com (Histologia@aol.com) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] "Old" Microtome Knives and Histology Equipment Message-ID: <1da.14b2c843.2cebe1d2@aol.com> Greetings! One of our volunteers referred this email posting on the Histoweb to me. We are American Ukrainian Medical Project and we would love to have more reliable steel knives for our microtomes which we send to Ukraine. Disposable knives are also welcome but we understand they are not always surplus. We can use knives of all sizes for all microtomes. You may ship them to us at: American Ukrainian Medical Project 302 - 156th Avenue NE Bellevue, WA 98007 Please mark the package "OK to leave at door". Send them by ordinary ground freight or USPS to keep costs down. Let us know if you need to be reimbursed for shipping costs. Our charity has had great success at obtaining certain donations but sadly cash is not one of them! If you absolutely must be reimbursed please write us first. Your 'old' steel knives will be sent to Ukraine shortly after the New Year along with our current stock of donated microtomes and assorted other equipment. There this equipment will enter a second life helping to improve the precision of Ukrainian histology and pathology. Combined with our introduction of IHC technology and antibodies we help to raise their accuracy from 50-80% to over 90%. We have been doing this for over five years - the effect of improving their equipment can be very dramatic. Anyone with precious surplus tissue processors or microtomes are urged to contact us, too. If it is in your way and no longer needed, we may be able to find a new home for these items. Shipping arrangements for heavier items are a little trickier, though, so again we ask you to write us to begin making these arrangements. To learn more about us I invite you to visit our website at www.aump.org or simply write back to "info@aump.org". Our website is about to get an overhaul so it may be unavailable for a short time. AUMP is an IRS-approved public charity organized under section 501(c)3 of the Internal revenue Code, so donations to us should be tax deductible. Your tax advisor can give you the full details. Please feel free to write us if you have any comments or questions. We look forward to hearing from you. Sincerely, Russ Ayers Executive Director American Ukrainian Medical Project www.aump.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/9ed58965/attachment.htm From asmith <@t> mail.barry.edu Tue Nov 18 15:22:58 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Book Search Message-ID: <494304423C63E246A5CF87A3AEEB577011EDA0@bumail01.barrynet.barry.edu> Barnes and Noble still has it. (At least, they say they have it.) -----Original Message----- From: Tracey Couse [mailto:tracey.couse@ibb.gatech.edu] Sent: Tuesday, November 18, 2003 4:53 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Book Search Hello to all, I just received notification from Amazon, that John Alan Kiernan's "Histological and Histochemical Methods: Theory and Practice" book is not available from any of their sources. Does anyone know from where I can purchase this? If not, can someone suggest an appropriate equivalent? I am interested in a book that will detail the chemical nature of dyes as well as their staining mechanisms...basically an extension of his talk at this year's NSH meeting. I am familiar with the "Best of the Histonet book list", but I am not sure of the best substitution(s). As always, thanks for any help. Tracey Couse Laboratory Coordinator/Histologist Georgia Tech/Emory Center for the Engineering of Living Tissues Georgia Institute of Technology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From lizchlipala <@t> premierhistology.com Tue Nov 18 15:39:01 2003 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] chondrotin sulfate and hyaluronic acid in decalcifed bone sections Message-ID: <000501c3ae1c$64d2cdc0$74d48a80@LIZ> Hello All Is it possible to stain specifically for chondrotin sulfate or hyaluronic acid in the cartilage of decalcified knee joints (guinea pig). I know that Alcian Blue ph 2.5 will stain for hyaluronic acid. I do have a previous post from John Kiernan that explains a pretreatment process for the Alcian Blue pH 2.5 to make it specific for hyaluronic acid. But I was wondering if anyone had any other suggestions and what I could do for staining of chondrotin sulfate. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031118/3cb58741/attachment.htm From carolb <@t> mail.phys.mcw.edu Tue Nov 18 16:43:10 2003 From: carolb <@t> mail.phys.mcw.edu (Carol Bobrowitz) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] ISBN # Message-ID: <59DF6640CDC0D7119DF000D0B761393C1A95A0@thor.phys.mcw.edu> Does anyone know the ISBN# for "Histological and Histochemical Methods: Theory and Practice" by John Alan Kiernan? Thank you Carol Ann Bobrowitz Physiology Medical College of Wisconsin cbobrowi@mcw.edu From scoop <@t> mail.nih.gov Tue Nov 18 17:19:35 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] Perl's stain Message-ID: Hi. When I do Perl's staining I never block endogenous peroxidase or heme groups. Does anyone have experience doing Perl's after blocking peroxidase (with H2O2 or other ways) and how do you do it and does the Perl's stain work ok afterwards? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From kb2drkprk <@t> yahoo.com Tue Nov 18 17:25:08 2003 From: kb2drkprk <@t> yahoo.com (Kelly Booher) Date: Fri Sep 16 15:22:13 2005 Subject: [Histonet] ISBN # In-Reply-To: <59DF6640CDC0D7119DF000D0B761393C1A95A0@thor.phys.mcw.edu> Message-ID: <20031118232508.50375.qmail@web20907.mail.yahoo.com> The ISBN# for "Histological and Histochemical Methods: Theory and Practice" is 0080249361 (according to Amazon.com). Kelly Booher, HTL (ASCP) Anatomic Pathology Swedish Medical Center, Providence Campus Seattle, WA 98122 --- Carol Bobrowitz wrote: > Does anyone know the ISBN# for "Histological and > Histochemical Methods: > Theory and Practice" by John Alan Kiernan? > > Thank you > Carol Ann Bobrowitz > Physiology > Medical College of Wisconsin > cbobrowi@mcw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From scoop <@t> mail.nih.gov Tue Nov 18 17:34:04 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies In-Reply-To: <200311111608.hABG843j014587@casbah.it.northwestern.edu> References: <200311111608.hABG843j014587@casbah.it.northwestern.edu> Message-ID: Hi. Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed paraffin embedded tissue. We used it on a transgenic mouse expressing Beta gal - pretty weak but detectable with ABC kit and HRP. I think most of the transgenics express weakly and I would consider using tyramide (TSA) if you need more signal because background is very low. I also think some of the Abcam anti-beta gal abs are pretty good (I don't know which ones, I borrowed them). Sharon >Has anyone had any experience with polyclonal antibody against >b-galactosidase? What reporter system have you used? Thank you. >Tom Kuzniar > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From gcallis <@t> montana.edu Tue Nov 18 17:49:02 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] eosinophils in mice Message-ID: <3.0.6.32.20031118164902.00be0e30@gemini.msu.montana.edu> John Tarpley wrote a wonderful article on staining eosinophils in Journal of Histotechnology back in the 80's, but is is a nice protocol. You can contact him for reference (I do not have it handy) at John Tarpley . Even though he is retired, he would be happy to help. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From darkdaym <@t> mindspring.com Tue Nov 18 17:52:09 2003 From: darkdaym <@t> mindspring.com (Mark Ray) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] ISBN # References: <59DF6640CDC0D7119DF000D0B761393C1A95A0@thor.phys.mcw.edu> Message-ID: <000601c3ae2e$fb5387f0$f188fea9@marka04yhe99ux> Colleagues, The US importer/distributor of Dr Kiernan's book, Histological and Histochemical Methods, is Oxford University Press, oup-usa.com. Their web site shows the ISBN as 0750649364. This is the correct ISBN. Their web site shows it to be in stock, but they don't offer discounts. It is too late to talk to them directly today, so I will call them in the morning to ascertain the stock status of this book. If it is in stock, discount dealers should be able to get it. If not, I will try to get a reliable estimate of when they expect it. I haven't always succeeded at that in the past. Til tomorrow. Mark Ray ----- Original Message ----- From: "Carol Bobrowitz" To: "'Histonet (E-mail)" Sent: Tuesday, November 18, 2003 4:43 PM Subject: [Histonet] ISBN # > Does anyone know the ISBN# for "Histological and Histochemical Methods: > Theory and Practice" by John Alan Kiernan? > > Thank you > Carol Ann Bobrowitz > Physiology > Medical College of Wisconsin > cbobrowi@mcw.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From darkdaym <@t> mindspring.com Tue Nov 18 17:57:11 2003 From: darkdaym <@t> mindspring.com (Mark Ray) Date: Fri Sep 16 15:22:14 2005 Subject: Fw: [Histonet] ISBN # Message-ID: <001401c3ae2f$af3bbe90$f188fea9@marka04yhe99ux> ----- Original Message ----- From: "Mark Ray" To: "Carol Bobrowitz" ; "'Histonet (E-mail)" Sent: Tuesday, November 18, 2003 5:52 PM Subject: Re: [Histonet] ISBN # > Colleagues, > > The US importer/distributor of Dr Kiernan's book, Histological and > Histochemical Methods, is Oxford University Press, oup-usa.com. Their web > site shows the ISBN as 0750649364. This is the correct ISBN. Their web > site shows it to be in stock, but they don't offer discounts. It is too > late to talk to them directly today, so I will call them in the morning to > ascertain the stock status of this book. If it is in stock, discount > dealers should be able to get it. If not, I will try to get a reliable > estimate of when they expect it. I haven't always succeeded at that in the > past. Til tomorrow. > > Mark Ray > > ----- Original Message ----- > From: "Carol Bobrowitz" > To: "'Histonet (E-mail)" > Sent: Tuesday, November 18, 2003 4:43 PM > Subject: [Histonet] ISBN # > > > > Does anyone know the ISBN# for "Histological and Histochemical Methods: > > Theory and Practice" by John Alan Kiernan? > > > > Thank you > > Carol Ann Bobrowitz > > Physiology > > Medical College of Wisconsin > > cbobrowi@mcw.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Wed Nov 19 00:55:07 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Re: chondroitin sulfate & hyaluronic acid (Long) References: <000501c3ae1c$64d2cdc0$74d48a80@LIZ> Message-ID: <3FBB13CB.FA228043@uwo.ca> A proper answer to Elizabeth's question depends on the detailed requirements of the investigation: just what questions do you need to answer? Does it really matter to be absolutely specific about which of the chondroitin sulphates? Must you be certain that the staining of hyaluronic acid is not being mimicked by another weakly acidic macromolecule? If rigorous specificity is needed, the following remarks will not be helpful. If it is sufficient to stain sulphated carbohydrates differently from big carbohydrates that are carboxylic acids, this can be done quite easily with dyes and other simple reagents. Several controls are always needed. They are all logically obvious, but also easily missed for reasons of expediency, impatience etc etc. Nearly all staining with dyes involves dye ions or molecules being attracted to and then binding to much larger molecules such as proteins, nucleic acids or macromolecular carbohydrates. The chondroitin sulphates are salts of a strong acid (sulphuric). As such, their macromolecular anions are always negatively charged, even at very low pH. Any cationic dye used at pH 1 will probably stain only the chondroitin sulphates in a section of cartilage. (I say probably because I don't know if cartilage also contains heparan or keratan sulphates, which are similar extracellular glycosaminoglycans of connective tissues.) Alcian blue at pH 1 is often used for this job. Hyaluronic acid is a weak acid, so the pH of a solution of a cationic dye needs to be 2.5 or higher for staining to occur. Alcian blue is better than other basic dyes for this application because it does not stain nucleic acids. (The reason is out there, in the Literature.) At pH 2.5, however, alcian blue stains the sulphated carbohydrates as well as the weaker acids such as hyaluronic. To stain the carboxyl items (like hyaluronate) selectively you must first remove the sulphate groups from chondroitin etc. Sulphate removal is a two-step process, and you will need to consult a histochemistry textbook for technical details. No unusual chemicals or apparatus are needed, but the procedures can remove sections from slides, so be sure to have plenty of sections. You may need to experiment with different ways of sticking the sections on the slides. The first step is METHYLATION. This permanently removes sulphate groups and reversibly blocks carboxyl groups. After methylation nothing should be stainable with any cationic dye at any pH. The second step is SAPONIFICATION. This restores methylated carboxyl groups but it cannot restore removed sulphate groups. Saponification is also a potent remover of sections from slides. After methylation and saponification, all staining of extracellular material by alcian blue is due to carboxylate anions of hyaluronic acid and/or similar macromolecules. The stainability of chondroitin sulphates cannot be restored by saponification. As an additional check, you can incubate some control sections in a solution of hyaluronidase, an enzyme that catalyses the hydrolysis of the huge hyaluronate polyanion into small, water-soluble fragments. If the enzyme treatment prevents the staining, it was probably hyaluronic acid. A control for the enzyme digestion is important, because staining may be prevented by digestion in water or a buffer. If this happens, the stainable material may or may not have been hyaluronic acid. There are chondroitinases, which might be exploited in the histochemical analysis of chondroitin sulphates. I don't think they have been investigated in the context of dye-based carbohydrate histochemistry. Please let me know if I've been missing some important developments in this field. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ____________________________________________ Elizabeth Chlipala asked: > Is it possible to stain specifically for chondrotin sulfate or hyaluronic acid in the cartilage of decalcified knee > joints (guinea pig). I know that Alcian Blue ph 2.5 will stain for hyaluronic acid. I do have a previous post > from John Kiernan that explains a pretreatment process for the Alcian Blue pH 2.5 to make it specific for > hyaluronic acid. But I was wondering if anyone had any other suggestions and what I could do for staining > of chondrotin sulfate. From alexander.nader <@t> wgkk.sozvers.at Wed Nov 19 01:26:08 2003 From: alexander.nader <@t> wgkk.sozvers.at (Nader, Alexander) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Triton vs. Tween Message-ID: <4FE843CBBAC1D211A8150008C70952DAAE7B61@hk01nt05.hkh.wgkk.sozvers.at> > An excellent booklet on detergents for biology is available > online from CalBioChem at: > > http://www.emdbiosciences.com/SharedImages/TechnicalLiterature/1_Detbooklet_ 2001.pdf Dear Tim, thank you for this tip. This book is excellent! Alexander Nader Vienna, Austria From slieblich <@t> shaw.ca Wed Nov 19 01:20:43 2003 From: slieblich <@t> shaw.ca (slieblich@shaw.ca) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] BrdU and NeuN protocol Message-ID: <5162c851b8b5.51b8b55162c8@shaw.ca> Hi, thanks for the help so far but... I'm still having problems with my triple immunofluorescence of brdu, neun and gfap. Does anyone have a protocol that works using these three antibodies for mouse tissue (or at least a double label protocol for BrdU and NeuN)? I'd really appreciate it. I think my problem lies between the BrdU protocol and NeuN. Thanks for any help! Stephanie Lieblich University of British Columbia From n.benabdallah <@t> anatom.unizh.ch Wed Nov 19 05:51:40 2003 From: n.benabdallah <@t> anatom.unizh.ch (Nada Ben Abdallah) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] why picric acid Message-ID: <3.0.6.32.20031119125140.01230a50@rzu-mailhost.unizh.ch> Hi again, sorry for keeping so long to answer your question. In fact the perfusion protocol that we are using in my lab could be called a ''multi-disciplinary'' one; with the sulfide we can do Timm staining; as for the picric acid, it helps to make cell membrane more penetrant therefore it is easier when doing thick sections to use it for the immunohistochemistry, for antibodies to enter the cells. Here you go; I hope I'm getting clearer and sorry for the confusion that I caused you. PS: with this sections picric acid is not necessary. Best wishes, nada From John.Auld <@t> whnt.nhs.uk Wed Nov 19 05:54:51 2003 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Re Book search Message-ID: Tracey Ihave just (18th) ordered John Kiernan's book for Amazon in the UK and it should be delivered in the next couple of days. It maybe worth trying Amazon.co.uk There are advantages to being on this side of the Atlantic. Regards John Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral Hello to all, I just received notification from Amazon, that John Alan Kiernan's "Histological and Histochemical Methods: Theory and Practice" book is not available from any of their sources. Does anyone know from where I can purchase this? If not, can someone suggest an appropriate equivalent? I am interested in a book that will detail the chemical nature of dyes as well as their staining mechanisms...basically an extension of his talk at this year's NSH meeting. I am familiar with the "Best of the Histonet book list", but I am not sure of the best substitution(s). As always, thanks for any help. Tracey Couse Laboratory Coordinator/Histologist Georgia Tech/Emory Center for the Engineering of Living Tissues Georgia Institute of Technology From siksik03 <@t> comcast.net Wed Nov 19 06:42:27 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Book Search In-Reply-To: <5.0.0.25.2.20031118152333.049fbae0@mail.ibb.gatech.edu> References: <5.0.0.25.2.20031118152333.049fbae0@mail.ibb.gatech.edu> Message-ID: Hi HistoNetters The Kiernan book is in stock new in paperback from ecampus.com or used at half.com. It is easy to find books- just do a search at addall.com. best regards, Steven Slap ****************************** Steven E. Slap Microwave Consultant (413) 221-3678 microwaves@comcast.net ***************************** At 3:53 PM -0600 11/18/03, Tracey Couse wrote: >Hello to all, > >I just received notification from Amazon, that John Alan Kiernan's >"Histological and Histochemical Methods: Theory and Practice" book >is not available from any of their sources. Does anyone know from >where I can purchase this? If not, can someone suggest an >appropriate equivalent? I am interested in a book that will detail >the chemical nature of dyes as well as their staining >mechanisms...basically an extension of his talk at this year's NSH >meeting. I am familiar with the "Best of the Histonet book list", >but I am not sure of the best substitution(s). As always, thanks >for any help. > >Tracey Couse >Laboratory Coordinator/Histologist >Georgia Tech/Emory Center for the > Engineering of Living Tissues >Georgia Institute of Technology > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sladd <@t> hsc.usf.edu Wed Nov 19 07:33:08 2003 From: sladd <@t> hsc.usf.edu (Sharron Ladd) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Re:ISBN # for Dr. Kiernan's book--also check medical library In-Reply-To: <59DF6640CDC0D7119DF000D0B761393C1A95A0@thor.phys.mcw.edu> References: <59DF6640CDC0D7119DF000D0B761393C1A95A0@thor.phys.mcw.edu> Message-ID: <3FBB7114.3080900@hsc.usf.edu> I have the second edition and the ISBN# is 0-08-036223-0. Also, our library has a copy maybe yours will?? Sharron University of South Florida Carol Bobrowitz wrote: >Does anyone know the ISBN# for "Histological and Histochemical Methods: >Theory and Practice" by John Alan Kiernan? > >Thank you >Carol Ann Bobrowitz >Physiology >Medical College of Wisconsin >cbobrowi@mcw.edu > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Ngilman <@t> nbhd.org Wed Nov 19 07:42:50 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] baby brain processing sched Message-ID: Hello everyone. I have to process baby brain (stillborns & miscarriages) for a project our newest pathologist is doing. It's been a long time (25+ years) since I've done any pediatric neuro tissue. Can anyone help me with suggested fixation/processing protocols? TIA Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/93e18316/attachment.htm From mhorne <@t> upei.ca Wed Nov 19 06:29:17 2003 From: mhorne <@t> upei.ca (Margaret Horne) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] hoaxes Message-ID: <3FBB37EC.12782.29CB16@localhost> When in doubt , I check this site www.urbanlegends.about.com covers everything from current net hoaxes and email rumours to medical/health hype and old scams, a huge range of topics. Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada From mrl0627 <@t> mail.ecu.edu Wed Nov 19 08:00:20 2003 From: mrl0627 <@t> mail.ecu.edu (mrl0627@mail.ecu.edu) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies Message-ID: Sharon, Have you used this antibody on previously X-gal reacted tissue? We are using 40-1a. Thanks, Maureen Loomer, MS candidate at ECU -----Original Message----- From: Sharon Cooperman <scoop@mail.nih.gov> To: t-kuzniar@md.northwestern.edu Sent: Tue, 18 Nov 2003 18:34:04 -0500 Subject: Re: [Histonet] Anti-beta-galactosidase antibodies Hi. Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed paraffin embedded tissue. We used it on a transgenic mouse expressing Beta gal - pretty weak but detectable with ABC kit and HRP. I think most of the transgenics express weakly and I would consider using tyramide (TSA) if you need more signal because background is very low. I also think some of the Abcam anti-beta gal abs are pretty good (I don't know which ones, I borrowed them). Sharon >Has anyone had any experience with polyclonal antibody against >b-galactosidase? What reporter system have you used? Thank you. >Tom Kuzniar > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sharon Cooperman <scoop@mail.nih.gov> NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From darkdaym <@t> mindspring.com Wed Nov 19 08:36:42 2003 From: darkdaym <@t> mindspring.com (Mark Ray) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Kiernan's Histological and Histochemical Methods Message-ID: <001e01c3aeaa$8d41d100$f188fea9@marka04yhe99ux> To confirm what others are reporting, I have spoken to my reliable and gracious contact in the Inventory Department at Oxford University Press/USA and he assures me that there are an ample number of copies of John Kiernan's Histological and Histochemical Methods in stock to meet current US demands. He sees no reason why any distributor/book dealer would be unable to obtain copies and does not expect it to go out of print in the near future. The holiday season is fast approaching and this would make a fine present for someone who doesn't own or have ready access to a copy. Any book dealer can get it, some of the larger ones stock it. The ISBN of the current paperback edition is 0750649364. Season's Greetings, Mark Ray -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/a267d3b2/attachment.htm From scoop <@t> mail.nih.gov Wed Nov 19 08:40:27 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies Message-ID: No, sorry. Just out of curiousity, why do you want to do both the enzymatic and IHC? Also, I think that if the enzyme isn't destroyed by the reaction it might work - eg., I wouldn't expect it to work on peroxidase, but I don't know if beta gal activity persists after the reaction. Sharon >Sharon, >Have you used this antibody on previously X-gal reacted tissue? We >are using 40-1a. Thanks, Maureen Loomer, MS candidate at ECU > >-----Original Message----- >From: Sharon Cooperman <scoop@mail.nih.gov> >To: t-kuzniar@md.northwestern.edu >Sent: Tue, 18 Nov 2003 18:34:04 -0500 >Subject: Re: [Histonet] Anti-beta-galactosidase antibodies > >Hi. Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed >paraffin embedded tissue. We used it on a transgenic mouse >expressing Beta gal - pretty weak but detectable with ABC kit and >HRP. I think most of the transgenics express weakly and I would >consider using tyramide (TSA) if you need more signal because >background is very low. I also think some of the Abcam anti-beta gal >abs are pretty good (I don't know which ones, I borrowed them). > >Sharon > >>Has anyone had any experience with polyclonal antibody against >>b-galactosidase? What reporter system have you used? Thank you. >>Tom Kuzniar >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >-- >Sharon Cooperman <scoop@mail.nih.gov> >NIH, NICHD, CBMB 301.435-7735 >Building 18T, room 101 301.402-0078 fax >Bethesda, MD 20892 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From lao_ji <@t> yahoo.com Wed Nov 19 09:21:20 2003 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Re: sticky column filter In-Reply-To: <20031118142459.10510.qmail@web25101.mail.ukl.yahoo.com> Message-ID: <20031119152120.53005.qmail@web12502.mail.yahoo.com> Hi, here let me take a chance saying thanks to Judith and Debby Grant for their kindly giving me advice. It seems to me the No.3) from Judith will help my situation. Even thought this histonet group traffic with huge amount email, you getting something useful is most important. Thanks again! Jimmy from a developmental Lab skirball institute NYU medical center --- Judith van Dommelen wrote: > Hello Jimmy, > I saw your question on the internet. If you still > have > problems, try one of the following: > 1) make a prerinse program with ethanol or methanol > (whatever u use for rehydration) and rinse the > columns > before loading your embryos > 2) Use heat permeation instead of proteinase K > treatment: after rehydration, place embryos in fresh > PBST in a 2 ml (cryo) tube, place them in a 95*C > waterbath for 5 min. and then snapcool on ice. > Replace > again with fresh PBST and place embryos back in > columns. I found that pK treatment damaged the > embryos > too much which made them more sticky > 3) Cover the column filter with Sephadex: Dissolve a > teaspoon of Sephadex G50 in 100 ml of deionized > RNA-free water, solve properly and then fill columns > till 1/3 of volume with the Sephadex solution. Let > columns drain in the machine, a gooey layer of > sephadex now covers the filter frit. This has no > negative effect on signal development. > > Good Luck\ > Judith > > We use 1% NaOH (Sodium Hydroxide) 6 hours to overnight, then we rinse 3x with distilled water, then soak them in distilled H20 overnight, then rinse 2x with distilled H20, then dry them in an oven @ 60 degrees Celsius, and rinse in 100% EtOH then dry again in the oven @ 60 degrees Celsius. Usually we can use the columns until the filters pop out from the columns, approximately 3-4 times. Also, if you use tween 20 at 0.1% in all hybridization solutions you shouldn't have this problem with the embryos sticking to the columns. Debby Grant Research Technician II Histology Core Facility Stowers Institute for Medical Research________________________________________________________________________ > Want to chat instantly with your online friends? > Get the FREE Yahoo! > Messenger http://mail.messenger.yahoo.co.uk __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From gcallis <@t> montana.edu Wed Nov 19 09:59:20 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies In-Reply-To: Message-ID: <3.0.6.32.20031119085920.00bcbde0@gemini.msu.montana.edu> Good point and my thoughts exactly. I would certainly try the BETA GAL Roche kit to develop lovely beta gal blue color before going to the antibody. I think one key thing is to have a good positive tissue control. Robin Taylor from Genentech sent me a wonderful protocol using this kit, I am sure she would not mind if I shared it privately. It is very straight forward. The only time we used the antibody was to detect beta gal for immunofluorescence. There ARE fluorescent beta gal substrates available but using antibody staining seems just as easy for this application. At 09:40 AM 11/19/2003 -0500, you wrote: >No, sorry. Just out of curiousity, why do you want to do both the >enzymatic and IHC? Also, I think that if the enzyme isn't destroyed >by the reaction it might work - eg., I wouldn't expect it to work on >peroxidase, but I don't know if beta gal activity persists after the >reaction. > >Sharon > >>Sharon, >>Have you used this antibody on previously X-gal reacted tissue? We >>are using 40-1a. Thanks, Maureen Loomer, MS candidate at ECU >> >>-----Original Message----- >>From: Sharon Cooperman <scoop@mail.nih.gov> >>To: t-kuzniar@md.northwestern.edu >>Sent: Tue, 18 Nov 2003 18:34:04 -0500 >>Subject: Re: [Histonet] Anti-beta-galactosidase antibodies >> >>Hi. Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed >>paraffin embedded tissue. We used it on a transgenic mouse >>expressing Beta gal - pretty weak but detectable with ABC kit and >>HRP. I think most of the transgenics express weakly and I would >>consider using tyramide (TSA) if you need more signal because >>background is very low. I also think some of the Abcam anti-beta gal >>abs are pretty good (I don't know which ones, I borrowed them). >> >>Sharon >> >>>Has anyone had any experience with polyclonal antibody against >>>b-galactosidase? What reporter system have you used? Thank you. >>>Tom Kuzniar >>> >>> >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>-- >>Sharon Cooperman <scoop@mail.nih.gov> >>NIH, NICHD, CBMB 301.435-7735 >>Building 18T, room 101 301.402-0078 fax >>Bethesda, MD 20892 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > >-- >Sharon Cooperman >NIH, NICHD, CBMB 301.435-7735 >Building 18T, room 101 301.402-0078 fax >Bethesda, MD 20892 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jkiernan <@t> uwo.ca Wed Nov 19 10:17:58 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] ISBN # References: <20031118232508.50375.qmail@web20907.mail.yahoo.com> Message-ID: <3FBB97B6.6B4DA84B@uwo.ca> Then Amazon.com have it wrong! The ISBN for the 3rd edition is 0750649364. The ISBN in Kelly Booher's email is for the 1st edition (1981). I'm surprised that Amazon have it; do they also sell 2nd hand books? -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ____________________________________ Kelly Booher wrote: > > The ISBN# for "Histological and Histochemical Methods: > Theory and Practice" is 0080249361 (according to > Amazon.com). > > Kelly Booher, HTL (ASCP) > Anatomic Pathology > Swedish Medical Center, Providence Campus > Seattle, WA 98122 > > --- Carol Bobrowitz wrote: > > Does anyone know the ISBN# for "Histological and > > Histochemical Methods: > > Theory and Practice" by John Alan Kiernan? > > > > Thank you > > Carol Ann Bobrowitz > > Physiology > > Medical College of Wisconsin > > cbobrowi@mcw.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > __________________________________ > Do you Yahoo!? > Protect your identity with Yahoo! Mail AddressGuard > http://antispam.yahoo.com/whatsnewfree > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kb2drkprk <@t> yahoo.com Wed Nov 19 10:33:53 2003 From: kb2drkprk <@t> yahoo.com (Kelly Booher) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] ISBN # In-Reply-To: <3FBB97B6.6B4DA84B@uwo.ca> Message-ID: <20031119163353.59198.qmail@web20906.mail.yahoo.com> Amazon.com is out of stock of the 3rd edition, but they have the 1st edition available as a "special order". Each edition has its own ISBN. Kelly Booher, HTL (ASCP) Anatomic Pathology Swedish Medical Center, Providence Campus Seattle, WA 98122 --- John Kiernan wrote: > Then Amazon.com have it wrong! > The ISBN for the 3rd edition is 0750649364. > > The ISBN in Kelly Booher's email is for > the 1st edition (1981). I'm surprised that > Amazon have it; do they also sell 2nd hand > books? > -- > ------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan@uwo.ca > http://publish.uwo.ca/~jkiernan/ > ____________________________________ > Kelly Booher wrote: > > > > The ISBN# for "Histological and Histochemical > Methods: > > Theory and Practice" is 0080249361 (according to > > Amazon.com). > > > > Kelly Booher, HTL (ASCP) > > Anatomic Pathology > > Swedish Medical Center, Providence Campus > > Seattle, WA 98122 > > > > --- Carol Bobrowitz > wrote: > > > Does anyone know the ISBN# for "Histological > and > > > Histochemical Methods: > > > Theory and Practice" by John Alan Kiernan? > > > > > > Thank you > > > Carol Ann Bobrowitz > > > Physiology > > > Medical College of Wisconsin > > > cbobrowi@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > __________________________________ > > Do you Yahoo!? > > Protect your identity with Yahoo! Mail > AddressGuard > > http://antispam.yahoo.com/whatsnewfree > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard http://antispam.yahoo.com/whatsnewfree From algranth <@t> u.arizona.edu Wed Nov 19 10:56:04 2003 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] ISBN # In-Reply-To: <3FBB97B6.6B4DA84B@uwo.ca> References: <20031118232508.50375.qmail@web20907.mail.yahoo.com> Message-ID: <4.3.2.7.2.20031119095350.00cb4040@algranth.inbox.email.arizona.edu> I bought your book on Amazon second hand about two years ago. It was the first edition. Andi Grantham At 11:17 AM 11/19/2003 -0500, you wrote: >Then Amazon.com have it wrong! >The ISBN for the 3rd edition is 0750649364. > >The ISBN in Kelly Booher's email is for >the 1st edition (1981). I'm surprised that >Amazon have it; do they also sell 2nd hand >books? >-- >------------------------- >John A. Kiernan >Department of Anatomy and Cell Biology >The University of Western Ontario >London, Canada N6A 5C1 > kiernan@uwo.ca > http://publish.uwo.ca/~jkiernan/ >____________________________________ >Kelly Booher wrote: > > > > The ISBN# for "Histological and Histochemical Methods: > > Theory and Practice" is 0080249361 (according to > > Amazon.com). > > > > Kelly Booher, HTL (ASCP) > > Anatomic Pathology > > Swedish Medical Center, Providence Campus > > Seattle, WA 98122 > > > > --- Carol Bobrowitz wrote: > > > Does anyone know the ISBN# for "Histological and > > > Histochemical Methods: > > > Theory and Practice" by John Alan Kiernan? > > > > > > Thank you > > > Carol Ann Bobrowitz > > > Physiology > > > Medical College of Wisconsin > > > cbobrowi@mcw.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > __________________________________ > > Do you Yahoo!? > > Protect your identity with Yahoo! Mail AddressGuard > > http://antispam.yahoo.com/whatsnewfree > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From bhewlett <@t> cogeco.ca Wed Nov 19 11:15:36 2003 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] RE: Histological &Histochemical Methods Message-ID: <003901c3aec0$c06145a0$6400a8c0@bryanmainbox> John is being modest, any edition of his text is a worthwhile addition to your library!!! A first edition of this text is more useful than many current Histology books and better than not having a copy at all. This has been a recommended text for Canadian student technologists for years. Bryan John Kiernan wrote: >>The ISBN in Kelly Booher's email is for >>the 1st edition (1981). I'm surprised that >>Amazon have it; do they also sell 2nd hand >>books? From mprice26 <@t> juno.com Wed Nov 19 11:16:07 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: <20031119.091618.12981.213821@webmail08.nyc.untd.com> Histonetters, What is the deadline for the HS Diploma/ 2 year OJT for becoming a Registered Histotech take place? Thank You. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From Terry.Marshall <@t> rothgen.nhs.uk Wed Nov 19 11:16:46 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] RE: Histological &Histochemical Methods Message-ID: Well, could anyone reading his posts doubt it? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 19 November 2003 17:16 To: kiernan@uwo.ca Cc: histonet netserver Subject: [Histonet] RE: Histological &Histochemical Methods John is being modest, any edition of his text is a worthwhile addition to your library!!! A first edition of this text is more useful than many current Histology books and better than not having a copy at all. This has been a recommended text for Canadian student technologists for years. Bryan John Kiernan wrote: >>The ISBN in Kelly Booher's email is for >>the 1st edition (1981). I'm surprised that >>Amazon have it; do they also sell 2nd hand >>books? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tflore <@t> lsuhsc.edu Wed Nov 19 11:37:57 2003 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: Marsha, I believe its 2005, and you have two opportunities left to send your practical, but someone please correct me if I am wrong. As of now, the Study Group for the Histotecnology Registry HT(ASCP) has nine who attend. We meet at LSUHSC, 1901 Perdido Street, 3rd floor, Seminar Room 8, once a month, (next study group meeting is December 15, 2003 8-1) under the leadership of Rosemary Velasquez. Rosemary is an excellent teacher and has great study tips. Teresa Flores -----Original Message----- From: mprice26@juno.com [mailto:mprice26@juno.com] Sent: Wednesday, November 19, 2003 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Histonetters, What is the deadline for the HS Diploma/ 2 year OJT for becoming a Registered Histotech take place? Thank You. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/7529a83d/attachment.htm From STapper <@t> slhduluth.com Wed Nov 19 12:20:55 2003 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] embedding like specimens sequentially Message-ID: <3BFBBD68413CB443A7125A63EC0ACD1D6716BA@slhw2smail01.slhdomain.com> Can anyone give me a literature reference that delineates the reasons and process for embedding non-like specimens sequentially? We are trying to address our PA's ordering cervical biopsies followed by endocervical curettage biopsies, and breast masses followed by breast core biopsies, etc.. The only thing that seems to drive change is documented literature, not histotechs telling them that this is poor practice, and potential a legal problem. I don't need case studies - WE all know them - I need hard and fast rules. Thank you in advance! Sheila Tapper HT(ASCP) St. Luke's Hospital -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/ee650701/attachment.htm From GrobeA <@t> saintpatrick.org Wed Nov 19 12:28:58 2003 From: GrobeA <@t> saintpatrick.org (Albert Grobe) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Rabbit Blood Flow Message-ID: I apologize for the non-histological nature of this question, but perhaps someone in Histoland can help me. I am looking for the normal rate of flow (ml/min) throught the abdominal aorta of rabbits (New Zealand Whites if possible). If you have a reference I can quote, even better. Thanks, Albert Albert C. Grobe, MS Tissue Engineering Lab International Heart Institute Missoula, MT 59802 Office: (406) 329-5634 E-Mail: GrobeA@saintpatrick.org From georgecole <@t> ev1.net Wed Nov 19 13:38:40 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] A Big Step For The New muscle/nerve procedures Message-ID: <000c01c3aed4$bd3ce410$074dbad0@hppav> Histotechs; 84 PACKETS SENT---AND A BIG STEP TAKENI One of those muscle and nerve packet going out all over has landed in a neuropathologist's hands and the good doctor is now studying the methods in his lab. He says he is getting good results. He has given me a wonderful lift with his acceptance of those methods for their actual study as possible inclusions in his lab's patient care. He also wrote some positive statements about the methods and the means of communicating them. I know the home-made videos are a bit crude, but I guess they do manage to communicate the How To's of the many improvements to muscle and nerve biopsy technique presented in them.. Folks, I've tried to say this before---and I don't want to be tiresome---I don't want people to press DELETE the moment they see my name on the histonet----but being aware of better ways to do things---especially when the things done are life and death patient services---and shrugging them off with a "Well, that's how he does it, but it isn't the way I do it!" is a big NO NO in my belief system. I automatically included, in my processing of patient tissues, ANY improvement in procedure, whether it was easier or harder or more expensive to do, or whatever!! I have been worried about the fate of these methods. I know we histotechs don't usually have the authority to schedule retraining time when new methods come along, nor could they usually just up and buy new equipment for new procedures. I was extraordinarily fortunate to work for Dr. D'Agostino. I ordered any supplies or equipment needed by each improvement as it came along, no questions asked. It did take some time for the big break-through cases to come along, but then, all was well. I was patiently reminded, sometimes, that I was taking more than the usual time to do biopsies. But the comparative completeness of the information I dug out of the tissues eventually helped to excuse me for the extra in-out time. I always tried to keep the process time to a minimum----it was quality and completeness first, and I just hoped the clock would forgive me if I couldn't do it this in the same time as the mediocre studies . I have heard from a few of you who are giving the new methods a try. Please, I hope I'm not being hard headed about this---but folks, shouldn't EVERYONE, who is informed about better procedures for of patient care, DO their enactments of those better procedures? . Please excuse this diatribe---on the next 23 messages on the Histonet, I will perform english music-hall songs and play catchy numbers on two spoons. georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/9ef6981e/attachment.htm From CTague <@t> ahs.llumc.edu Wed Nov 19 13:40:29 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] embedding like specimens sequentially Message-ID: I'd have your pathologist put the hammer down. He or she would no doubt be happy to see you're desire to improve quality control and reduce the chance any mix up that could lead to legal problems. Explain to them the risks and I'm sure they'll back you. curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tapper, Sheila Sent: Wednesday, November 19, 2003 10:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding like specimens sequentially Can anyone give me a literature reference that delineates the reasons and process for embedding non-like specimens sequentially? We are trying to address our PA's ordering cervical biopsies followed by endocervical curettage biopsies, and breast masses followed by breast core biopsies, etc.. The only thing that seems to drive change is documented literature, not histotechs telling them that this is poor practice, and potential a legal problem. I don't need case studies - WE all know them - I need hard and fast rules. Thank you in advance! Sheila Tapper HT(ASCP) St. Luke's Hospital Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/c9742ec0/attachment.htm From froyer <@t> bitstream.net Wed Nov 19 13:48:24 2003 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Hacker Down Draft Hood Message-ID: <3FBBC908.4030902@bitstream.net> The Histotechnology Program at a local community college is looking for a couple of Hacker Down Draft Hoods for their student labs. They do have a limited budget and will be willing & able to pay for these. If you have a surplus down-draft hood that you no longer are using, please contact me "Off Line". Thanks, ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, Inc. 1200 Mendelssohn Ave. N., Ste. 50 Minneapolis, MN 55427-4366 (800) 565-1895 phone (612) 804-1015 Cell Phone (952) 929-1895 Fax email -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/0660b790/attachment.htm From fmonson <@t> wcupa.edu Wed Nov 19 14:01:29 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:14 2005 Subject: FW: [Histonet] Rabbit Blood Flow Message-ID: Try: White, S.w., et al., (1967), Aust. J. Exp. Biol. Med. Sci., 45: 453. Hepatic blood flow: Method: Thermal dilution. Ml/min=100 or ml/min/100g liver = 37 (portal vein) Data from: p. 1705, Biology Data Book, 2d Ed., Vol III, 1974, Federation of American Societies for Experimental Biology, Bethesda, MD (FASEB) Library of Congress: Qh310 .A392 Hope this helps, FCM Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Albert Grobe [mailto:GrobeA@saintpatrick.org] Sent: Wednesday, November 19, 2003 1:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Rabbit Blood Flow I apologize for the non-histological nature of this question, but perhaps someone in Histoland can help me. I am looking for the normal rate of flow (ml/min) throught the abdominal aorta of rabbits (New Zealand Whites if possible). If you have a reference I can quote, even better. Thanks, Albert Albert C. Grobe, MS Tissue Engineering Lab International Heart Institute Missoula, MT 59802 Office: (406) 329-5634 E-Mail: GrobeA@saintpatrick.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CCLYATT <@t> mail.mcg.edu Wed Nov 19 14:03:46 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] embedding like specimens sequentially Message-ID: You might also contact you legal department. I'm sure they would take great interest. Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> "Tague, Curtis" 11/19/03 02:40PM >>> I'd have your pathologist put the hammer down. He or she would no doubt be happy to see you're desire to improve quality control and reduce the chance any mix up that could lead to legal problems. Explain to them the risks and I'm sure they'll back you. curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tapper, Sheila Sent: Wednesday, November 19, 2003 10:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding like specimens sequentially Can anyone give me a literature reference that delineates the reasons and process for embedding non-like specimens sequentially? We are trying to address our PA's ordering cervical biopsies followed by endocervical curettage biopsies, and breast masses followed by breast core biopsies, etc.. The only thing that seems to drive change is documented literature, not histotechs telling them that this is poor practice, and potential a legal problem. I don't need case studies - WE all know them - I need hard and fast rules. Thank you in advance! Sheila Tapper HT(ASCP) St. Luke's Hospital Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From HornHV <@t> archildrens.org Wed Nov 19 14:28:17 2003 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] embedding like specimens sequentially Message-ID: To help avoid issues with like specimens we color code them. The first case of GI will be yellow, the next orange, the next pink...etc. The resident dictates the color of the cassette as well. It works great for a small lab like ours. We then follow with putting the yellow casettes on yellow slides, orange on orange slide....etc. It does help us as we get many GI biopsies. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Tague, Curtis [mailto:CTague@ahs.llumc.edu] Sent: Wednesday, November 19, 2003 1:40 PM To: Tapper, Sheila Cc: Histonet (E-mail) Subject: RE: [Histonet] embedding like specimens sequentially I'd have your pathologist put the hammer down. He or she would no doubt be happy to see you're desire to improve quality control and reduce the chance any mix up that could lead to legal problems. Explain to them the risks and I'm sure they'll back you. curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tapper, Sheila Sent: Wednesday, November 19, 2003 10:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding like specimens sequentially Can anyone give me a literature reference that delineates the reasons and process for embedding non-like specimens sequentially? We are trying to address our PA's ordering cervical biopsies followed by endocervical curettage biopsies, and breast masses followed by breast core biopsies, etc.. The only thing that seems to drive change is documented literature, not histotechs telling them that this is poor practice, and potential a legal problem. I don't need case studies - WE all know them - I need hard and fast rules. Thank you in advance! Sheila Tapper HT(ASCP) St. Luke's Hospital Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/ccbbea74/attachment.htm From tpmorken <@t> labvision.com Wed Nov 19 14:38:51 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] embedding like specimens sequentially Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD3C@usca0082k03.rallansci.apogent.com> Shiela, That's what QA programs are for. The problem is that even if you can document one or more mistakes you can't really say for sure if it is because of sequential grossing of like specimens unless you have data showing that. It could just be coincidental. You would have to show that such mistakes happen predominately when like specimens are grossed sequentially. If you have a QA program you can make this potential problem part of that as a QA monitor in order to document the problem. Followup on all mis-identification mistakes (and be sure to document those that are caught in the lab - not just those that reach a pathologist) and determine their place in the grossing order (is that possible with your methods?). Then follow trends of mistakes and try to assign the trend to specific practices. That is the only way to show the magnitude of any problem. Tim Morken Lab Vision / NeoMarkers www.labvision.com -----Original Message----- From: Tague, Curtis [mailto:CTague@ahs.llumc.edu] Sent: Wednesday, November 19, 2003 11:40 AM To: Tapper, Sheila Cc: Histonet (E-mail) Subject: RE: [Histonet] embedding like specimens sequentially I'd have your pathologist put the hammer down. He or she would no doubt be happy to see you're desire to improve quality control and reduce the chance any mix up that could lead to legal problems. Explain to them the risks and I'm sure they'll back you. curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tapper, Sheila Sent: Wednesday, November 19, 2003 10:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding like specimens sequentially Can anyone give me a literature reference that delineates the reasons and process for embedding non-like specimens sequentially? We are trying to address our PA's ordering cervical biopsies followed by endocervical curettage biopsies, and breast masses followed by breast core biopsies, etc.. The only thing that seems to drive change is documented literature, not histotechs telling them that this is poor practice, and potential a legal problem. I don't need case studies - WE all know them - I need hard and fast rules. Thank you in advance! Sheila Tapper HT(ASCP) St. Luke's Hospital Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/f474b4ee/attachment.htm From cfavara <@t> niaid.nih.gov Wed Nov 19 14:42:15 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Rabbit Blood Flow Message-ID: Albert, St. Pats has a pretty good library and I believe they have pub med access you should be able to find the information there. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Albert Grobe [mailto:GrobeA@saintpatrick.org] Sent: Wednesday, November 19, 2003 11:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Rabbit Blood Flow I apologize for the non-histological nature of this question, but perhaps someone in Histoland can help me. I am looking for the normal rate of flow (ml/min) throught the abdominal aorta of rabbits (New Zealand Whites if possible). If you have a reference I can quote, even better. Thanks, Albert Albert C. Grobe, MS Tissue Engineering Lab International Heart Institute Missoula, MT 59802 Office: (406) 329-5634 E-Mail: GrobeA@saintpatrick.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MinHan.Tan <@t> vai.org Wed Nov 19 16:32:59 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Frozen section fixing problems - but paraffin works! Message-ID: <74D0F0AB07F2E647A02D839ED79520F94A9DB2@VAIEXCH02.vai.org> Hi, I am working with a new monoclonal antibody in parathyroid tissue, and I use the ABC-DAB reageant system for immunohistochemistry. I am new to frozen sections immunostaining - and I am encountering problems with frozen sections - staining is very weak or absent, in comparison to paraffin embedded tissue, which I have no problems with. My protocol for frozen sections: Unfixed frozen slides with 5 micron sections thawed at room temperature for 5 minutes Placed in 70% ethanol x 5 minutes. Washed in PBS x 5 minutes. ... followed with standard protocol: hydrogen peroxide 0.3% x 30 min, donkey serum 5% x 30 min, primary antibody (mouse) 4 deg overnight, secondary (goat anti-mouse), ABC, DAB. Does anyone have any advice? Thank you! Regards, Min-Han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/ef86360c/attachment.htm From MinHan.Tan <@t> vai.org Wed Nov 19 17:00:55 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Frozen section fixing problems - but paraffin works! Message-ID: <74D0F0AB07F2E647A02D839ED79520F97B9B96@VAIEXCH02.vai.org> Sorry, I neglected to mention - I have also tried Sections were cut in cryostat and stored as unfixed slides overnight in -80. (no dessication though) Unfixed frozen slides (stored at -80) placed at -20 for 5 minutes Placed in -20 acetone for 5 minutes. Air dried at room temperature for 10 minutes As per standard protocol. -----Original Message----- From: Tan, MinHan Sent: Wednesday, November 19, 2003 5:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen section fixing problems - but paraffin works! Hi, I am working with a new monoclonal antibody in parathyroid tissue, and I use the ABC-DAB reageant system for immunohistochemistry. I am new to frozen sections immunostaining - and I am encountering problems with frozen sections - staining is very weak or absent, in comparison to paraffin embedded tissue, which I have no problems with. My protocol for frozen sections: Unfixed frozen slides with 5 micron sections thawed at room temperature for 5 minutes Placed in 70% ethanol x 5 minutes. Washed in PBS x 5 minutes. ... followed with standard protocol: hydrogen peroxide 0.3% x 30 min, donkey serum 5% x 30 min, primary antibody (mouse) 4 deg overnight, secondary (goat anti-mouse), ABC, DAB. Does anyone have any advice? Thank you! Regards, Min-Han Tan _____ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. _____ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/c8bdf1f4/attachment.htm From haldana <@t> unimoron.edu.ar Wed Nov 19 17:09:08 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Frozen section fixing problems - but paraffin works! References: <74D0F0AB07F2E647A02D839ED79520F94A9DB2@VAIEXCH02.vai.org> Message-ID: <018601c3aef2$2453dba0$7504a8c0@um.edu> MessageDear Tan You have a lot of advaices in the wonderfull manual of Technical Immunohistochemistry of Rodney Miller, go to www.propathlab.com and download it free.Or go to http://hjaldanamarcos.bravepages.com/documentos.htm and donwload the documents named INMUNO1.... to INMUNO5. By Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: Tan, MinHan To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 19, 2003 7:32 PM Subject: [Histonet] Frozen section fixing problems - but paraffin works! Hi, I am working with a new monoclonal antibody in parathyroid tissue, and I use the ABC-DAB reageant system for immunohistochemistry. I am new to frozen sections immunostaining - and I am encountering problems with frozen sections - staining is very weak or absent, in comparison to paraffin embedded tissue, which I have no problems with. My protocol for frozen sections: Unfixed frozen slides with 5 micron sections thawed at room temperature for 5 minutes Placed in 70% ethanol x 5 minutes. Washed in PBS x 5 minutes. ... followed with standard protocol: hydrogen peroxide 0.3% x 30 min, donkey serum 5% x 30 min, primary antibody (mouse) 4 deg overnight, secondary (goat anti-mouse), ABC, DAB. Does anyone have any advice? Thank you! Regards, Min-Han Tan ------------------------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. ------------------------------------------------------------------------------ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/8c9099cb/attachment.htm From ccdub <@t> earthlink.net Wed Nov 19 17:11:12 2003 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #133 - 27 msgs In-Reply-To: <20031117180001.1506.48862.Mailman@swlx167.swmed.edu> Message-ID: There are 3 histolabs around my area and all of us start no later than 4 a.m. on 11/17/03 10:00 AM, histonet-request@lists.utsouthwestern.edu at histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-admin@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. what is histology (Michael F.) > 2. re what is histology? (Carl) > 3. RE: Perfusion pump time (Charles W. Scouten, Ph.D.) > 4. RE: e-cadherin antibody (Scott Mordue) > 5. Re: e-cadherin antibody (SAULSAULX@aol.com) > 6. Re: Decreased antigenicity with alcohol-formalin fixation (anita dudley) > 7. Re: e-cadherin antibody (SAULSAULX@aol.com) > 8. Trichromes (was Re:What is Histology?) (John Kiernan) > 9. cytokeratin (Lombaert) > 10. Re: Exhaust Block (louise renton) > 11. RE: thick frozen sections (Alan Bright) > 12. Re: Trichromes (was Re:What is Histology?) (SAULSAULX@aol.com) > 13. Re: Trichromes (was Re:What is Histology?) (rfail@toolkitmail.com) > 14. Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?) (Ian > Montgomery) > 15. Re: cytokeratin (Dennis Najarian) > 16. Re: Trichromes (was Re:What is Histology?) (Bryan Hewlett) > 17. Hansson's Carbnic Anhydrase. (leanneharris@eircom.net) > 18. What is histology? (Dawson, Glen) > 19. RE: Anti-beta-galactosidase antibodies (Favara, Cynthia (NIH/NIAID)) > 20. RE: Hansson's Carbnic Anhydrase. (Houston, Ronnie) > 21. polymerization failure _ Technovit 9100New (Tom Schaer) > 22. Re: Re: [Histonet] Trichromes (Hernan Aldana Marcos) > 23. Trichromes and "ten-page double-spaced paper" (Alex Knisely) > 24. trichrome paper (Mary Lou) > 25. Re: Trichromes (was Re:What is Histology?) (Gayle Callis) > 26. Re: CSAII kit (Dana Settembre) > 27. RE: re what is histology? (Chung, Luong) > > --__--__-- > > Message: 1 > To: histonet@lists.utsouthwestern.edu > From: "Michael F." > Organization: Waikiki Coffee Inc. > Date: Sun, 16 Nov 2003 09:31:58 -1000 > Subject: [Histonet] what is histology > > as an aside, could you folks tell what percent would you say of HT/HTL > start work before 5am? > > would you say that an HT/HTL would be more likely to have opportunities to > work in research than a MT/MTL? > > I'm considering one or the other schools. Hope you don't mind my off topic > posting. Please email me directly if its more appropriate. > > cheers, > > M. > > ...back to lurking > > > --__--__-- > > Message: 2 > From: "Carl" > To: > Date: Sun, 16 Nov 2003 19:52:55 -0000 > Subject: [Histonet] re what is histology? > > After a friend asked what I am/did, I expained about Histology, staining, > dyes etc. I subsequently overheard him saying to another person that I was > an artist! To him dyes/stains= paints...( hadn't listened to a word....or my > explanation was inadequate lol) > I suppose it's true; a picture paints a thousand words. Perhaps that's why, > for example, the pathologist uses many words to describe what's seen in a > section ;-) > > > > --__--__-- > > Message: 3 > Date: Sun, 16 Nov 2003 17:20:17 -0600 > From: "Charles W. Scouten, Ph.D." > To: "Davis, Gareth" , > "Histonet" > Subject: RE: [Histonet] Perfusion pump time > > This is a multi-part message in MIME format. > > ------_=_NextPart_001_01C3AC98.32688D72 > Content-Type: text/plain; > charset="us-ascii" > Content-Transfer-Encoding: quoted-printable > > I have responded separately with an attached 7 page discussion of > perfusion, and a link to our Perfusion One product. Don't use flow > rate, pressure is what the cardiovascular system controls, use 5% > sucrose prewash, no saline, and switch to fixative when the animal > stops. Haparin is not needed, all blood can be cleared without it with > sufficient pressure. > > =20 > > If any body else would like to see these materials, I will send them. > > =20 > > Charles W. Scouten, Ph.D.=20 > myNeuroLab.com=20 > 5918 Evergreen Blvd.=20 > St. Louis, MO 63134=20 > Ph: 314 522 0300 =20 > FAX 314 522 0377=20 > cwscouten@myneurolab.com =20 > www.myneurolab.com=20 > > =20 > > -----Original Message----- > From: Davis, Gareth [mailto:gareth.davis@Vanderbilt.Edu]=20 > Sent: Saturday, November 15, 2003 3:29 PM > To: Histonet > Subject: [Histonet] Perfusion pump time > > =20 > > Hello Histonetters, > > Recently (within the last couple months) I saw someone post perfusion > times for pumps based on the animal being perfused. I tried to find the > posting on the Histosearch, but unsuccessful. We are perfusing rats for > the olfactory tissue, but would like ideals on the what others have > done. Any input would be appreciated, including if heparin is needed in > saline, the correct perfusion pump time, and if recirculation is > necessary. > > thanks, > > Gareth > > =20 > > Ms. Gareth B. Davis > > Research Assistant II > > =20 > > =20 > > > ------_=_NextPart_001_01C3AC98.32688D72 > Content-Type: text/html; > charset="us-ascii" > Content-Transfer-Encoding: quoted-printable > > > > > > charset=3Dus-ascii"> > > > > > > > > >
> >

Roman"> style=3D'font-size:12.0pt;color:navy'>I have responded separately with = > an attached >  7 page discussion of perfusion, and a link to our Perfusion One > product.  Don’t use flow rate, pressure is what the = > cardiovascular > system controls,  use 5% sucrose prewash, no saline, and switch to = > fixative > when the animal stops.  Haparin is not needed, all blood can be = > cleared without > it with sufficient pressure.

> >

Roman"> style=3D'font-size:12.0pt;color:navy'> 

> >

Roman"> style=3D'font-size:12.0pt;color:navy'>If any body else would like to see = > these > materials, I will send them.

> >

Roman"> style=3D'font-size:12.0pt;color:navy'> 

> >
> >

style=3D'font-size:12.0pt; > font-family:"Comic Sans MS";color:navy'>Charles W.  Scouten, = > Ph.D. color=3Dnavy>
> style=3D'color:#339966;font-weight: > bold'>myNeuroLab.com style=3D'color:navy'> >
> Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>5918 > Evergreen Blvd. style=3D'color:navy'>
> Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>St. Louis, > MO 63134 = >
> Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>Ph: 314 522 > 0300  style=3D'color:navy'>
> Style"> style=3D'font-size:10.0pt;font-family:"Bookman Old = > Style";color:navy'>FAX  > 314 522 0377 = >
> cwscouten@myneurolab.com = >
> www.myneurolab.com

> >
> >

Roman"> style=3D'font-size:12.0pt;color:navy'> 

> >

face=3DTahoma> style=3D'font-size:10.0pt;font-family:Tahoma'>-----Original = > Message-----
> From: Davis, Gareth > [mailto:gareth.davis@Vanderbilt.Edu]
> Sent: Saturday, November = > 15, 2003 > 3:29 PM
> To: Histonet
> Subject: [Histonet] = > Perfusion pump > time

> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>Hello Histonetters,

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>Recently (within the last couple months) I = > saw someone > post perfusion times for pumps based on the animal being = > perfused.  I > tried to find the posting on the Histosearch, but unsuccessful.  We = > are > perfusing rats for the olfactory tissue, but would like ideals on the = > what > others have done.  Any input would be appreciated, including if = > heparin is > needed in saline, the correct perfusion pump time, and if > recirculation is necessary.

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>thanks,

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'>Gareth

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >
> >

color=3Dpurple > face=3DSystem> style=3D'font-size:10.0pt;font-family:System;color:purple'>Ms. > Gareth B. Davis

> >
> >
> >

size=3D2 > color=3Dpurple face=3DSystem> style=3D'font-size:10.0pt;font-family:System; > color:purple'>Research Assistant II

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >
> >

face=3D"Times New Roman"> style=3D'font-size:12.0pt'> 

> >
> >
> > > > > =00 > ------_=_NextPart_001_01C3AC98.32688D72-- > > > --__--__-- > > Message: 4 > Date: Sun, 16 Nov 2003 18:03:16 -0800 (PST) > From: Scott Mordue > To: alexander.nader@wgkk.sozvers.at > Cc: Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] e-cadherin antibody > > We are using monoclonal E-Cadherin in breast cancer > tissues, clone: 4A2C7, from Zymed. > > Scott > CSU > > > From: "Nader, Alexander" > > To: "'histonet-admin@lists.utsouthwestern.edu'" > > Cc: "'Histonet@lists.utsouthwestern.edu'" > > Date: Wed, 12 Nov 2003 08:07:01 +0100 > Subject: RE: [Histonet] e-cadherin antibody > >> Which clone are people using for e-caherin IHC on > FFPE tissues? >> Novocastra E-Cadherin clone 36B5 (IgG1) 1:50. > > Alexander Nader > Inst. f. Pathology, Hanusch-Hospital > Vienna, Austria > > __________________________________ > Do you Yahoo!? > Protect your identity with Yahoo! Mail AddressGuard > http://antispam.yahoo.com/whatsnewfree > > > --__--__-- > > Message: 5 > From: SAULSAULX@aol.com > Date: Sun, 16 Nov 2003 21:12:34 EST > To: i_stain@yahoo.com, alexander.nader@wgkk.sozvers.at > CC: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] e-cadherin antibody > > > --part1_1a5.1c1895a5.2ce98892_boundary > Content-Type: text/plain; charset="US-ASCII" > Content-Transfer-Encoding: 7bit > > Histotechies......! I need help.where can i get a ten page double space > research paper on the following two stains: > > 1.Alcian Blue > > 2.Trichrome stain > > > thanks > > --part1_1a5.1c1895a5.2ce98892_boundary > Content-Type: text/html; charset="US-ASCII" > Content-Transfer-Encoding: quoted-printable > > =3D"Arial" LANG=3D"0">Histotechies......! I need help.where can i > get=20= > a ten page double space research paper on the following two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks > > --part1_1a5.1c1895a5.2ce98892_boundary-- > > > --__--__-- > > Message: 6 > From: "anita dudley" > To: GoodwinD@pahosp.com, HistoNet@Pathology.swmed.edu > Date: Mon, 17 Nov 2003 02:13:03 +0000 > Subject: Re: [Histonet] Decreased antigenicity with alcohol-formalin fixation > > diana, I use penfix on my processor and we have good luck with our ers and > prs, we also use it sometime to fix breast tissue before going on the > processor. haven't had any problems with it. hope this helps. > anita dudley > providence hosp > mobile alabama > > >> From: "Goodwin, Diana" >> To: "Histonet (E-mail)" >> Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation >> Date: Fri, 14 Nov 2003 11:39:21 -0500 >> >> >>> Greetings, Histonetters. >>> >>> One of my pathologists has expressed concern that antigenicity for >> certain >>> antibodies, specifically ER and PR, in breast tissue that is processed >>> using alcohol-formalin will be decreased. Can anyone cite a reference >> in >>> the literature that confirms or addresses this concern? Has anyone had >>> this experience, or does anyone know of any reason that using >>> alcohol-formalin (specifically Pen-Fix) would adversely affect IHC >>> staining? >>> >>> Thanks to all of you, >>> >>> Diana Goodwin >>> Supervisor, Anatomic Pathology >>> Preston 6 >>> 215-829-6532 >>> e-mail: goodwind@pahosp.com >>> > > _________________________________________________________________ > Frustrated with dial-up? Get high-speed for as low as $26.95. > https://broadband.msn.com (Prices may vary by service area.) > > > > --__--__-- > > Message: 7 > From: SAULSAULX@aol.com > Date: Sun, 16 Nov 2003 21:15:04 EST > To: SAULSAULX@aol.com, i_stain@yahoo.com, alexander.nader@wgkk.sozvers.at > CC: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] e-cadherin antibody > > > --part1_28.40452994.2ce98928_boundary > Content-Type: text/plain; charset="US-ASCII" > Content-Transfer-Encoding: 7bit > > In a message dated 11/16/2003 9:14:01 PM Eastern Standard Time, > SAULSAULX@aol.com writes: > >> Histotechies......! I need help.where can i get a ten page double space >> research paper on the following two stains: >> >> 1.Alcian Blue >> >> 2.Trichrome stain >> >> >> thanks > > Histotechies......! I need help.where can i get a ten page double space > research paper on the following two stains: > > 1.Alcian Blue > > 2.Trichrome stain > > > thanks > > --part1_28.40452994.2ce98928_boundary > Content-Type: text/html; charset="US-ASCII" > Content-Transfer-Encoding: quoted-printable > > =3D"Arial" LANG=3D"0">In a message dated 11/16/2003 9:14:01 PM Eastern Stand= > ard Time, SAULSAULX@aol.com writes:
>
>
: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">Histotechies......! I nee= > d help.where can i get a ten page double space research paper on the followi= > ng two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks ZE=3D3 FAMILY=3D"SANSSERIF" FACE=3D"arial" LANG=3D"0">
= >
> FAMILY=3D"SANSSERIF" FACE=3D"Arial" LANG=3D"0">
> Histotechies......! I need help.where can i get a ten page double spac= > e research paper on the following two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks ZE=3D3 FAMILY=3D"SANSSERIF" FACE=3D"arial" LANG=3D"0">
> > --part1_28.40452994.2ce98928_boundary-- > > > --__--__-- > > Message: 8 > Date: Mon, 17 Nov 2003 00:47:47 -0500 > From: John Kiernan > Reply-To: jkiernan@uwo.ca > To: SAULSAULX@aol.com > CC: Jackie.O'Connor@abbott.com, tigersnake@ecybermind.net, > histonet@lists.utsouthwestern.edu > Subject: [Histonet] Trichromes (was Re:What is Histology?) > > The last 2 paragraphs of this email are not > about trichrome staining. > > Are you (SAULSAULX@aol.com) looking for a > review of postulated mechanisms of differential > staining in the various trichrome methods, or > for 10 pages of practical instructions, or > for comparisons of different trichrome methods? > If you let us know what you need you (and > other Histonetters) will probably get several > reading lists! > > The staining mechanisms are controversial > because there has been too much speculation > and not enough research. In this field you'll > need to read 3 or 4 review articles to try > to form a balanced view of your own. The > mechanisms for trichromes (methods using 3 > anionic dyes and PTA or PMA) need to be > considered alongside methods using 2 > anionic dyes in one solution (notably > Van Gieson's stain and its less often > used congeners). > > Is your name really SAULSAULX? It's much > more satisfying to reply to a name at a > real location such as, for example, > Abey C. Defghi at the Royal Spithouse Infirmary > in Badchester, Rayneshire, England. > Histonet is a friendly community of over > 1000 (I think; Herb or Linda might correct > the number), and most of us indicate who we are > when we ask or answer questions. > > On this note (and nothing to do with trichrome > stains), I'd like to say how happy I was to > see many Histonetters face to face at the > recent NSH meeting in Louisville. I wish I > could have talked with more of you, but it's > easy to miss people in a big crowd. Also, the > names on the lapel badges were printed too > small. Such badges should be readable at a > distance. Though not a shy person, I did not > want to stare closely at the name label on > every comely bosom. In these litiginous times > everyone must avoid being greatly misunderstood. > (NSH label makers please take note! Not many > people have telescopic vision. Our instrument > is the microscope.) From SJones <@t> cvm.tamu.edu Wed Nov 19 17:27:01 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] embedding like specimens sequentially Message-ID: This is an intriguing problem. I'm unsure why anyone would court disaster if it could be avoided. I checked the web page http://www.pathologistsassistants.org/ PA's do work under the direction of the pathologist so as Curtis pointed out, that would be one way to handle it. Also, the web site states under the code of regulation: Section 3. Service. A Pathologists' Assistant is qualified by academic and practical training to provide the following services under the direction and supervision of a pathologist: A. Preparation, gross description and dissection of human tissue surgical specimens including: 1. Assuring appropriate specimen accessioning. Since this is a bit ambiguous, it could be put in your procedure manual as an SOP and then all the fussin an feudin would be put to an end. I'm puttin that "fussin and feudin" lingo in their for all our UK friends that have been so privileged to hear our fearless leader (cough, cough) speak this week. He's very brave when it comes to bombing and sending the troops in, but Members of parliament are just too much for him! ;) Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Tague, Curtis" 11/19/2003 1:40:29 PM >>> I'd have your pathologist put the hammer down. He or she would no doubt be happy to see you're desire to improve quality control and reduce the chance any mix up that could lead to legal problems. Explain to them the risks and I'm sure they'll back you. curt -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tapper, Sheila Sent: Wednesday, November 19, 2003 10:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding like specimens sequentially Can anyone give me a literature reference that delineates the reasons and process for embedding non-like specimens sequentially? We are trying to address our PA's ordering cervical biopsies followed by endocervical curettage biopsies, and breast masses followed by breast core biopsies, etc.. The only thing that seems to drive change is documented literature, not histotechs telling them that this is poor practice, and potential a legal problem. I don't need case studies - WE all know them - I need hard and fast rules. Thank you in advance! Sheila Tapper HT(ASCP) St. Luke's Hospital Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From SJones <@t> cvm.tamu.edu Wed Nov 19 17:58:46 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] replacement belt for a Sorvall JB-4 microtome Message-ID: Does anyone know where a replacement belt (for the motor) can be found for a Sorvall JB-4 microtome. Apparently these are harder to find than hen's teeth. Any help would be appreciated. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From robint <@t> mediom.qc.ca Wed Nov 19 18:54:58 2003 From: robint <@t> mediom.qc.ca (Robin Turcotte) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Bielschowsky's stain Message-ID: <002201c3af00$ec9bcc40$9b8ceccf@client> Hi Does anyone have good result with BIELSCHOWSK'S STAIN ? I am using the technique from Bancroft as follows on brain tissue of dogs: 1. Deparaffinize and hydrate to distilled water. Wash well in distilled water. 2. Place sections in 50 ml of 20% silver nitrate in the dark at 37 C. Keep this silver solution for stage 4. 3. Wash well in distilled water. 4. Add strong ammonia to 50 ml of silver nitrate (from stage 2) until the initial precipitate disappears. Leave section in this solution for 10 minutes at 37 C. I would to understand step 4. Should I add concentrated ammonia? I added concentrated ammonia 1 to 2 drop at a time. Immediately I got precipitate that did not disappear. I repeated this step with dilute ammonia getting the same result. I filtered the soluton and continued the staining and obtained no color on my axons. I guess I have a problem with step 4. How much volume of ammonia should be added at what concentration. Is there a critical point in this staining. Thank you Robin Robint@mediom.qc.ca -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/3ad9bf19/attachment.htm From GREYTRUNK <@t> aol.com Wed Nov 19 19:38:23 2003 From: GREYTRUNK <@t> aol.com (GREYTRUNK@aol.com) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: <14b.2700dd8f.2ced750f@aol.com> I believe that you have until Dec 31, 2004 to sign up for the registry. Roxanne -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/a1637820/attachment.htm From newhistotech <@t> yahoo.com Wed Nov 19 22:02:23 2003 From: newhistotech <@t> yahoo.com (brade, brade upland, upland) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Biotin block after primary? Message-ID: <20031120040223.74538.qmail@web20702.mail.yahoo.com> Hi, I accidently added my primary antibody before avidin/biotin blocking. Is it possible to block after the primary but before a biontinylated secondary? Thank you for help. --------------------------------- Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031119/41329b44/attachment.htm From SJones <@t> cvm.tamu.edu Wed Nov 19 22:06:45 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Bielschowsky's stain Message-ID: Hi Robin, If you have never seen this done before, it may be confusing to you. Use concentrated ammonium hydroxide (less than one year old) and keep adding it until the solution clears. It takes quite a bit of ammonium hydroxide to get it to clear, but it will. I'm not sure of the amount because I just use a transfer pipet and keep adding it. Constantly swirl the flask as you add the ammonia to the silver. At the end it goes fast so watch for that. It's lots of chemistry fun and one of my favorite solutions to make up. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Robin Turcotte" 11/19/2003 6:54:58 PM >>> Hi Does anyone have good result with BIELSCHOWSK'S STAIN ? I am using the technique from Bancroft as follows on brain tissue of dogs: 1. Deparaffinize and hydrate to distilled water. Wash well in distilled water. 2. Place sections in 50 ml of 20% silver nitrate in the dark at 37 C. Keep this silver solution for stage 4. 3. Wash well in distilled water. 4. Add strong ammonia to 50 ml of silver nitrate (from stage 2) until the initial precipitate disappears. Leave section in this solution for 10 minutes at 37 C. I would to understand step 4. Should I add concentrated ammonia? I added concentrated ammonia 1 to 2 drop at a time. Immediately I got precipitate that did not disappear. I repeated this step with dilute ammonia getting the same result. I filtered the soluton and continued the staining and obtained no color on my axons. I guess I have a problem with step 4. How much volume of ammonia should be added at what concentration. Is there a critical point in this staining. Thank you Robin Robint@mediom.qc.ca From antje.marcantonio <@t> pharma.novartis.com Thu Nov 20 04:43:39 2003 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Frozen section fixing problems - but paraffin works! Message-ID: Hello Min-Han Tan you are not writing which primary antibody you use. And how you dilute the 0.3 % hydrogen peroxide, in a buffer (like TBS or PBS) or methanol ? Just a thought: 0.3 % perox block in buffer for 30 min could be too harsh. The use of methanol, which is no problem in formalin fixed tissue , might destroy the epitope in frozens. Regards, Antje Marcantonio Novartis Pharma AG BU Transplantation Research Basel, Switzerland -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/dd21270f/attachment.htm From livieira <@t> ualg.pt Thu Nov 20 05:33:36 2003 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Thanks for your help! Message-ID: <001001c3af5a$2307ff70$2914100a@labhistologia> I wanted to thank everyone who take me information about Sudan Black an Oil Red O staining. Thanks, Lina Vieira Universidade do Algarve Faro, Portugal -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/4934d8c3/attachment.htm From c.m.vanderloos <@t> amc.uva.nl Thu Nov 20 05:34:35 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] RE: Frozen section fixing problems - but paraffin works! Message-ID: <7e571d7e8fe3.7e8fe37e571d@amc.uva.nl> Dear Tan, Your protocol for staining the cryostat sections needs some adaptions indeed. First, cut your sections and allow them to dry at least 1 hour at RT. Preferably longer, for example over night under a fan. Next, fix your cryo's in pure acetone for 10 min at 4C, let them air-dry. Block endogenous peroxidase activity with 0.3% perhydrol + 0.1% Na-azide in TBS or PBS (20 min, RT) and wash with TBS or PBS (3 x 2 min). Then, start with the donkey serum etc. and your usual staining procedure. Some comments: You should avoid the use of alcohols or methanol in the staining procedure, because most antigens will be damaged by this. Blocking with 0.3% peroxide (in buffer I hope??) is not recommended because the formation of air bubbles that may damage your tissue section. The addition of Na-azide prevents the formation of air bubbles and also acts as inhibitor of peroxidase activity. Good luck with staining! Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From "Tan, MinHan" Date Wed, 19 Nov 2003 17:32:59 -0500 To Subject [Histonet] Frozen section fixing problems - but paraffin works! Hi, I am working with a new monoclonal antibody in parathyroid tissue, and I use the ABC-DAB reageant system for immunohistochemistry. I am new to frozen sections immunostaining - and I am encountering problems with frozen sections - staining is very weak or absent, in comparison to paraffin embedded tissue, which I have no problems with. My protocol for frozen sections: Unfixed frozen slides with 5 micron sections thawed at room temperature for 5 minutes Placed in 70% ethanol x 5 minutes. Washed in PBS x 5 minutes. followed with standard protocol: hydrogen peroxide 0.3% x 30 min, donkey serum 5% x 30 min, primary antibody (mouse) 4 deg overnight, secondary (goat anti-mouse), ABC, DAB. Does anyone have any advice? Thank you! Regards, Min-Han Tan From khor0011 <@t> flinders.edu.au Thu Nov 20 06:18:41 2003 From: khor0011 <@t> flinders.edu.au (khor0011@flinders.edu.au) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Thanks everyone for the help! Message-ID: <1069330721.3fbcb121ee6e0@imap.flinders.edu.au> To people who have helped, Just want to say thankyou to people who have given me information on "snap freezing in liquid nitrogen with isopentene". I have looked into histo- textbooks and i finally found the information i wanted. Thanks again, i would not have known the information as i have no histology background and my Honours supervisor could not help me with this aspect as well. Cheers Yuan Flinder's University South Australia From tissuearray <@t> hotmail.com Thu Nov 20 06:43:55 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Manual embedding of tissue cores for arrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/5ddc86f5/attachment.htm From w.huang <@t> qmul.ac.uk Thu Nov 20 07:19:16 2003 From: w.huang <@t> qmul.ac.uk (wenlong huang) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] anti active caspase-3 Message-ID: <003b01c3af68$e5ebc7c0$ce4c258a@imap.qmul.ac.uk> Hi all, I am working on cell death after spinal cord injury in rats and want to try antibodies aganist active form of caspase-3 on 4%PF perfusion fixed cryosections of rat spinal cord. Does anyone know any effective antibody and protocols? Thanks very much, Wenlong Department of Neuroscience Queen Mary University of London Mile End Road, E1 4NS -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/542e329a/attachment.htm From NSEARCY <@t> swmail.sw.org Thu Nov 20 07:59:12 2003 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] New 2004 CPT Code Message-ID: <03Nov20.075943cst.119104@healthcare2.sw.org> There is a new surgical pathology CPT code- 88361- tumor immunocytochemistry (eg, Her-2/neu, estrogen/progesterone receptor) quantitative or semiquantitative, each antibody- could someone give me examples of the test names that this would be referring ? There is confusion as to just what is being conveted here. Thanks From JWEEMS <@t> sjha.org Thu Nov 20 08:09:43 2003 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] New 2004 CPT Code Message-ID: <9E75DAB5F369D84ABF84FAB7A0243B44B6D65F@exch4.sjha.org> I believe it replaces 88358 for the digitalized assay testing such as the Chromavision system. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Nita Searcy Sent: Thursday, November 20, 2003 8:59 AM To: histonet@pathology.swmed.edu Subject: [Histonet] New 2004 CPT Code There is a new surgical pathology CPT code- 88361- tumor immunocytochemistry (eg, Her-2/neu, estrogen/progesterone receptor) quantitative or semiquantitative, each antibody- could someone give me examples of the test names that this would be referring ? There is confusion as to just what is being conveted here. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ngilman <@t> nbhd.org Thu Nov 20 08:11:08 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] New 2004 CPT Code Message-ID: Nita, I believe this code is intended for Image Analysis. I think it states "morphometric analysis". Hope this helps. Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager >>> "Nita Searcy" 11/20/2003 8:59:12 AM >>> There is a new surgical pathology CPT code- 88361- tumor immunocytochemistry (eg, Her-2/neu, estrogen/progesterone receptor) quantitative or semiquantitative, each antibody- could someone give me examples of the test names that this would be referring ? There is confusion as to just what is being conveted here. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/3313db97/attachment.htm From sebres <@t> comcast.net Thu Nov 20 08:54:18 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Golgi-Cox Method References: <7C8114EB.6BA2E730.0082A251@aol.com> Message-ID: <000b01c3af76$2d3e3880$0300a8c0@homepc1> I'm attaching a protocol developed by my student, Tracey Wheeler, at George Mason University. I've seen her results and they're gorgeous! Best of luck, Susan Bachus ----- Original Message ----- From: To: Sent: Tuesday, November 18, 2003 8:43 AM Subject: [Histonet] Golgi-Cox Method > I was wondering if anyone out there had a staining protocol for Golgi-Cox. My principle investigator is having me investigate kits for this stain. We have found a source, but I also want to see other protocols out there as well. > > Thanks in advance, > > Steven R. Westra > University of Iowa Health Care > (319) 356-1090 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- A non-text attachment was scrubbed... Name: Golgi Cox Protocol[1].doc Type: application/msword Size: 35840 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/0bb7135c/GolgiCoxProtocol1.doc From FreidaC <@t> aol.com Thu Nov 20 09:09:40 2003 From: FreidaC <@t> aol.com (FreidaC@aol.com) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: <117.2bd3bab8.2cee3334@aol.com> I do not believe it is correct that you have until December 31, 2004 to sign up for the ASCP certifying exam. Without an associate degree, you must TAKE exam for the first time before December 31, 2004. This obviously means that you must register for it much earlier. Freida Carson From hagler.herb <@t> pathology.swmed.edu Thu Nov 20 09:33:43 2003 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Regarding HTML encoded email and unwanted attachments, An Update Message-ID: It would be so much simpler if everyone that is using their email client would simply learn how to send plain text email messages. That simple step would eliminate a lot of the trash code that appears in everyone's mail messages and in the digests. All mail clients do have this as an option, consult your local geek if you can't find it. In the meantime I have checked with our group on campus who are providing the list server space. It turns out that they are presently running an older version of the list software that does not currently block attachments and does not successfully remove the html garbage code. They are planning to update the list server software by mid December so please do not send attachments in your postings to the list. This includes the option to attach your vcard identity. After the update we will be able to eliminate the attachments and the html junk that comes with some people not using plain text message encoding. Thanks for your help and understanding. Have a great weekend.... Herb Hagler From mari.ann.mailhiot <@t> leica-microsystems.com Thu Nov 20 09:54:55 2003 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Bielschowsky's stain Message-ID: Robin I have a very nice Bielchowsky's method I used at University of Chicago. It always worked. If you like I can email it to you. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Robin Turcotte" To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] Bielschowsky's stain western.edu 11/19/2003 06:54 PM Hi Does anyone have good result with BIELSCHOWSK'S STAIN ? I am using the technique from Bancroft as follows on brain tissue of dogs: 1. Deparaffinize and hydrate to distilled water. Wash well in distilled water. 2. Place sections in 50 ml of 20% silver nitrate in the dark at 37 C. Keep this silver solution for stage 4. 3. Wash well in distilled water. 4. Add strong ammonia to 50 ml of silver nitrate (from stage 2) until the initial precipitate disappears. Leave section in this solution for 10 minutes at 37 C. I would to understand step 4. Should I add concentrated ammonia? I added concentrated ammonia 1 to 2 drop at a time. Immediately I got precipitate that did not disappear. I repeated this step with dilute ammonia getting the same result. I filtered the soluton and continued the staining and obtained no color on my axons. I guess I have a? problem with step 4. How much volume of ammonia should be added at what concentration. Is there a critical point in this staining. Thank you Robin Robint@mediom.qc.ca ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ From sebres <@t> comcast.net Thu Nov 20 09:53:24 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:14 2005 Subject: Golgi Cox x Re: [Histonet] Regarding HTML encoded email and unwanted attachments, An Update References: Message-ID: <000401c3af7e$6eb71c20$0300a8c0@homepc1> My apologies if in my ignorance I committed a faux pas in sending Tracey Wheeler's Golgi Cox protocol as an attachment--trying again here with plain text in hopes that this helps (ps Tracey can be reached at twheele2@gmu.edu if you have questions): Golgi Cox Protocol (Tracey's way.there are others as well, so don't be nervous if you come across them!) Step 1: Prepare the Golgi-Cox solution Solution A: 5% Potassium Dichromate in distilled H2O 200ml distilled H2O + 10 grams Potassium Dichromate (Mix in a glass beaker using a glass rod - best to do under fume hood) Solution B: 5% Mercuric Chloride (sublimate) in distilled H2O 200ml distilled H2O + 10 grams Mercuric Chloride (Mix in a glass beaker using a glass rod) (Mix solution on top of hotplate (on 5), stirring until dissolved) (Must be done under fume hood) Solution C: 5% Solution of Potassium Chromate in distilled H2O 160ml distilled H2O + 8 grams Potassium Chromate (Mix in a glass beaker using a glass rod - best to do under fume hood) Mix Solution A and Solution B into a 500ml glass beaker. Mix Solution C and 400ml of distilled H2O into a 1,000ml + glass beaker. Slowly pour the AB Solution into the C Solution while stirring continuously with a glass rod. Store in a glass stoppered bottle for 5 days in the dark. Note: You can easily manipulate the quantity of solution. Just make sure to follow these ratio's 5 Volume parts of 5% Potassium Dichromate solution 5 Volume parts of 5% Mercuric Chloride solution 4 Volume parts of 5% Potassium Chromate solution 10 Volume parts of H20 (to add to PC solution) Step 2: Transfer Golgi-Cox solution into small glass bottles. Use a plastic syringe to remove the GC solution from the large glass bottle(s). Be sure to avoid the reddish precipitate that formed on the top and bottom of the bottle. Glass bottles should be filled about ? full (to save room for 1 rat brain). Step 3: Sacrifice Rats using Saline Perfusion Technique. Deeply anaesthetize the animal. Place on an empty breeder box with wire top. (to allow blood to drain into box) Open chest cavity to expose heart. Insert 60 ml syringe filled with 9% saline into bottom right chamber of the heart. (This would be the animal's left chamber) Using scissors, cut the bottom left chamber of the heart open. (This would be the animal's right chamber) Begin to slowly push saline through the animal's system until the blood leaving the left chamber is clear. (This may take 3 syringes of saline.) When fluids are clear, decapitate and remove brain. Drop whole brain into prepared bottle(s) of Golgi Cox solution. Place in the dark for 14 days, refresh solution after 2 days. Step 4: Transfer Brains into Sucrose Solution. Mix 300 grams of Sucrose into 1000ml of distilled H2O. Place Beaker over hotplate and stir (using stir bar) until dissolved. Cool in refrigerator. (Once cool, ready to use.) Empty GC solution from jar and place brain on chem. wipe paper. Slightly blot. Rinse jar in distilled H2O, and refill with Sucrose Solution ? full. (In order to save room for brain.) Place brain in jar with Sucrose Solution. Brain will Float. Place jar(s) in refrigerator. (Once brains sink, they are ready to be sectioned.) (Brains should be sectioned within 2 months of transfer into sucrose.) Step 5: Section using a Vibratome. Prepare razor blade by immersion in xylene for 5 minutes to remove any traces of oil. (This should be done under the fume hood.) Wipe blade dry. Prepare a 6% sucrose solution. (6 grams sucrose in 100ml distilled H2O.) Mix well and make sure it is at room temperature or below before using. Fill the vibratome reservoir with the 6% Sucrose solution until the blade is covered. Mount brain section (up to ? a full brain) onto vibratome platform using superglue. (Make sure tissue is adhered well before sectioning, 5-7 minutes or more.) Insert platform (with adhered brain section) into reservoir. Set the vibratome speed and amplitude around the midpoint for sectioning (adjust as necessary for your specific machine and comfort level.) Section at 200 micro meters or desired thickness. (Sections over 400 may be difficult to analyze.) Using a small paintbrush coax the section onto a gelatinized slide. Cover tissue with Parafilm. Place slide on flat surface covered with bibulous paper. Place another sheet of bibulous paper over paraflim. Place your palm over the section an press down slightly, being careful not alter movement. (Your goal is to press the section into the gelatin on the slide so it will adhere to slide during staining.) Remove bibulous paper and place in humidity chamber. Note: We used a water sleeved incubator. In this apparatus it is necessary to keep the parafilm on the section. We also placed the slides on plastic trays which also held capfuls of water to be sure the slides did not dry out. Do not keep the slides in storage (humidity chamber or water incubator) for more than 4 days.) Step 6: Staining Prepare fresh solutions (enough to cover all slides): Distilled H2O (3 total) Ammonium Hydroxide (1 total - keep under fume hood!) Kodak Fix (1 total - keep under fume hood, mix in dark) 50% alcohol (1 total) 70% alcohol (1 total) 95% alcohol (1 total) 100% alcohol (3 total) CXA solution (1 total - keep under fume hood!) Kodak Fix Solution: Prepare all ingredients in beakers Mix in order: (do not mix in light) 1010 ml distilled H2O (add) 251 ml Kodak Fix solution A (add) 28 ml Kodak Fix solution B (add) 2020 ml distilled H2O CXA Solution: 1000 ml Chloroform 1000 ml Xylene 1000 ml 100% Alcohol (you can manipulate amounts, just use 1/3 each) Remove parafilm from slides if necessary and place in slide rack. Dip according to process below: 1. Distilled H2O for 1 minute 2. Ammonium Hydroxide for 30 minutes (IN THE DARK) (Using a darkroom light mix the Kodak Fix solution now.) 3. Distilled H2O for 1 minute (Best to just keep lights off) 4. Kodak Fix solution for 30 minutes (IN THE DARK) 5. Distilled H2O for 1 minute (once in H2O you can turn on lights) 6. 50% alcohol 1 minute 7. 70% alcohol 1 minute 8. 95% alcohol 1 minute 9. 100% alcohol 5 minutes 10.100% alcohol 5 minutes 11.100% alcohol 5 minutes 12.CXA 15 minutes (Keep under fume hood) (Keep slides in CXA under fume hood while cover slipping, pull one slide out at a time.) Note: change gloves often - they will disintegrate in CXA. Step 7: Coverslip with permount and lie out to dry. If possible all cover slipping should be done under fume hood. Slides should be allowed to remain under fume hood for 24 hours - lying flat. Pull one slide out of CXA at a time. Using a glass dropper, place 2 drops of permount on top of tissue. (Sections dry quickly, do not remove slide from CXA and allow to sit in air for more than 20 seconds.) Place glass coverslip over section, being careful to avoid trapping air bubbles. Note: Too little permount could allow tissue to dry out, too much will cause coverslip to slide off - monitor your work for these problems. Place slide on absorbent paper (we use white tray liners - usually under rat cages) Allow slides to lie flat for 24 hours. Slides can now be moved into slide boxes for storage. KEEP BOXES OPEN!!! Slides need to continue to dry for 6 months before analysis should be attempted. ----- Original Message ----- From: "Herb Hagler" To: Sent: Thursday, November 20, 2003 10:33 AM Subject: [Histonet] Regarding HTML encoded email and unwanted attachments, An Update > It would be so much simpler if everyone that is using their email > client would simply learn how to send plain text email messages. That > simple step would eliminate a lot of the trash code that appears in > everyone's mail messages and in the digests. All mail clients do have > this as an option, consult your local geek if you can't find it. > > In the meantime I have checked with our group on campus who are > providing the list server space. It turns out that they are presently > running an older version of the list software that does not currently > block attachments and does not successfully remove the html garbage > code. > > They are planning to update the list server software by mid December so > please do not send attachments in your postings to the list. This > includes the option to attach your vcard identity. > > After the update we will be able to eliminate the attachments and the > html junk that comes with some people not using plain text message > encoding. > > Thanks for your help and understanding. Have a great weekend.... > > Herb Hagler > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Nov 20 10:01:00 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Cardboard boxes. Message-ID: <6.0.0.22.2.20031120155613.02ce4480@udcf.gla.ac.uk> A request for UK subscribers. I'm looking for small cardboard boxes ~3x2.5x2 inches (8x6.5x5 cm's). I use these for storing blocks, both LM & EM. Anything similar would be fine. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/b29afb77/attachment.htm From tissuearray <@t> hotmail.com Thu Nov 20 10:18:18 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Manual array blocks - 2mm derma needle Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/78153483/attachment.htm From peggy <@t> nsh.org Thu Nov 20 10:44:25 2003 From: peggy <@t> nsh.org (Peggy Micciche) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech In-Reply-To: <117.2bd3bab8.2cee3334@aol.com> Message-ID: <002c01c3af85$8f786890$7300000a@histo2> You must apply by July 1, 2004 to be eligible to take the exam via Route 3 which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at www.ascp.org for more information. Peggy Micciche -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of FreidaC@aol.com Sent: Thursday, November 20, 2003 10:10 AM To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech I do not believe it is correct that you have until December 31, 2004 to sign up for the ASCP certifying exam. Without an associate degree, you must TAKE exam for the first time before December 31, 2004. This obviously means that you must register for it much earlier. Freida Carson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Hedley.Glencross <@t> CMMC.nhs.uk Thu Nov 20 10:35:17 2003 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] RE: Histonet digest, Vol 1 #139 - 17 msgs Message-ID: > FAX 314 522 0377=20 >Message: 2 Date: Wed, 19 Nov 2003 17:27:01 -0600 From: "Sarah Jones" To: , Cc: Subject: RE: [Histonet] embedding like specimens sequentially This is an intriguing problem. I'm unsure why anyone would court disaster if it could be avoided. I checked the web page http://www.pathologistsassistants.org/ PA's do work under the direction of the pathologist so as Curtis pointed out, that would be one way to handle it. Also, the web site states under the code of regulation: Section 3. Service. A Pathologists' Assistant is qualified by academic and practical training to provide the following services under the direction and supervision of a pathologist: A. Preparation, gross description and dissection of human tissue surgical specimens including: 1. Assuring appropriate specimen accessioning. Since this is a bit ambiguous, it could be put in your procedure manual as an SOP and then all the fussin an feudin would be put to an end. I'm puttin that "fussin and feudin" lingo in their for all our UK friends that have been so privileged to hear our fearless leader (cough, cough) speak this week. He's very brave when it comes to bombing and sending the troops in, but Members of parliament are just too much for him! ;) Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 Hi Sarah Next thing you know, we all (we'all) will be "whoopin and a hollerin" over here too!!!!!!!!!!! Regards Hedley Glencross Manchester Cytology Centre UK -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: application/ms-tnef Size: 5954 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/3d47f3be/attachment.bin From gcallis <@t> montana.edu Thu Nov 20 10:40:35 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] RE: Frozen section fixing problems - but paraffin works! In-Reply-To: <7e571d7e8fe3.7e8fe37e571d@amc.uva.nl> Message-ID: <3.0.6.32.20031120094035.00bd3910@gemini.msu.montana.edu> Tan, Chris van der Loos's suggestions are superb. You can buy a peroxidase block from Dako that is designed for frozen sections, Perox Block, S2001 - with sodium azide, in a buffer with 0.03% hydrogen peroxide. I works very well without chewing your sections off the slide, ready to use in a dropper bottle. and we use it for convenience with most of our frozen section animal peroxidase IHC. Drying frozen sections is critical! After acetone fixation, we go in front of the fan for 20 mins or so, to insure no residual acetone is present, sections are dry. Good luck, Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) At 12:34 PM 11/20/2003 +0100, you wrote: >Dear Tan, >Your protocol for staining the cryostat sections needs some adaptions >indeed. >First, cut your sections and allow them to dry at least 1 hour at RT. >Preferably longer, for example over night under a fan. Next, fix your >cryo's in pure acetone for 10 min at 4C, let them air-dry. Block >endogenous peroxidase activity with 0.3% perhydrol + 0.1% Na-azide in >TBS or PBS (20 min, RT) and wash with TBS or PBS (3 x 2 min). Then, >start with the donkey serum etc. and your usual staining procedure. > >Some comments: >You should avoid the use of alcohols or methanol in the staining >procedure, because most antigens will be damaged by this. >Blocking with 0.3% peroxide (in buffer I hope??) is not recommended >because the formation of air bubbles that may damage your tissue >section. The addition of Na-azide prevents the formation of air bubbles >and also acts as inhibitor of peroxidase activity. > >Good luck with staining! >Chris van der Loos >Dept. of Cardiovascular Pathology >Academical Medical Center >Amsterdam - The Netherlands > > >----- Original Message ----- >>From "Tan, MinHan" >Date Wed, 19 Nov 2003 17:32:59 -0500 >To >Subject [Histonet] Frozen section fixing problems - but paraffin >works! > >Hi, >I am working with a new monoclonal antibody in parathyroid tissue, and >I use the ABC-DAB reageant system for immunohistochemistry. > >I am new to frozen sections immunostaining - and I am encountering >problems with frozen sections - staining is very weak or absent, in >comparison to paraffin embedded tissue, which I have no problems with. > >My protocol for frozen sections: >Unfixed frozen slides with 5 micron sections thawed at room temperature >for 5 minutes >Placed in 70% ethanol x 5 minutes. >Washed in PBS x 5 minutes. > >followed with standard protocol: hydrogen peroxide 0.3% x 30 min, >donkey serum 5% x 30 min, primary antibody (mouse) 4 deg overnight, >secondary (goat anti-mouse), ABC, DAB. > >Does anyone have any advice? >Thank you! > >Regards, >Min-Han Tan > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Rcartun <@t> harthosp.org Thu Nov 20 10:51:01 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] New 2004 CPT Code Message-ID: Doesn't "88342" include interpretation? Why do you need to do independent quantitation resulting in an additional charge? Richard Cartun Director, Immunopathology Hartford Hospital >>> "Weems, Joyce" 11/20/03 09:09AM >>> I believe it replaces 88358 for the digitalized assay testing such as the Chromavision system. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Nita Searcy Sent: Thursday, November 20, 2003 8:59 AM To: histonet@pathology.swmed.edu Subject: [Histonet] New 2004 CPT Code There is a new surgical pathology CPT code- 88361- tumor immunocytochemistry (eg, Her-2/neu, estrogen/progesterone receptor) quantitative or semiquantitative, each antibody- could someone give me examples of the test names that this would be referring ? There is confusion as to just what is being conveted here. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernaweston <@t> hotmail.com Thu Nov 20 11:11:24 2003 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] separated cells Message-ID: Dear Histonet, My Pathologist would like to see a perfect sheet of cells. He wants the vacuoles and smashed sells eliminated. We have tried many different processing programs and have changed the temperature of the water on the stainer. I have sent pictures of the problems with formalin fixed skin (cut at 4 microns) and a bone marrow cut at 3 microns) If anyone has any suggestions I would be very grateful. The pictures are listed as separated cells 1 & 2 @ histonet pictures. Bernadette Weston, HT Histology Supervisor Barberton Citizens Hospital barberton OH _________________________________________________________________ From Terry.Marshall <@t> rothgen.nhs.uk Thu Nov 20 11:49:16 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] separated cells Message-ID: I can't understand this post. Is it just me? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk Dear Histonet, My Pathologist would like to see a perfect sheet of cells. He wants the vacuoles and smashed sells eliminated. We have tried many different processing programs and have changed the temperature of the water on the stainer. I have sent pictures of the problems with formalin fixed skin (cut at 4 microns) and a bone marrow cut at 3 microns) If anyone has any suggestions I would be very grateful. The pictures are listed as separated cells 1 & 2 @ histonet pictures. Bernadette Weston, HT Histology Supervisor Barberton Citizens Hospital barberton OH From mprice26 <@t> juno.com Thu Nov 20 11:58:06 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Temp Agencies in Dallas area Message-ID: <20031120.095816.24069.222881@webmail15.nyc.untd.com> Histonetters, Are there any Temp Staffing agencies that cover the Dallas area only? Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From DEllenburg2 <@t> stfrancishealth.org Thu Nov 20 13:37:04 2003 From: DEllenburg2 <@t> stfrancishealth.org (DEllenburg2@stfrancishealth.org) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] Cost Analysis For Producing An H&E Slide Message-ID: <6661F19BB774D711942900A0C905F2533D8A78@GVL01MSX> Histonetters.......! I need your help. I have been asked to do the cost analysis for producing an H&E slide. If you have had to do this and wouldn't mind sharing any of your information with me I would greatly appreciate. Thanks for any help in advance. Deborah Ellenburg HT (ASCP) Bon Secours St. Francis Hospital Greenville, SC phone: 864-255-1278 fax: 864-255-1664 e-mail: DEllenburg2@stfrancishealth.org The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at (864) 255-1000, and permanently delete the original e-mail, attachment(s), and any copies. From c.m.vanderloos <@t> amc.uva.nl Thu Nov 20 13:45:07 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] RE: Biotin block after primary? Message-ID: <85942e8578f8.8578f885942e@amc.uva.nl> Hi Brade, As as long as you haven't applied the biotinylated secondary antibody and third step streptavidin-reagent, you don't have any problem with biotin blocking after the primary antibody. The trick of usual 2-step biotin-blocking kits is to bind (strept) avidin to the intrinsic biotin in the tissue section first and then to saturate the left-over binding sites at the (strept)avidin molecule with free d-biotin. Good luck Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From "brade, brade upland, upland" Date Wed, 19 Nov 2003 20:02:23 -0800 (PST) To histonet@lists.utsouthwestern.edu Subject [Histonet] Biotin block after primary? Hi, I accidently added my primary antibody before avidin/biotin blocking. Is it possible to block after the primary but before a biontinylated secondary? Thank you for help. From gcallis <@t> montana.edu Thu Nov 20 13:55:12 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:22:14 2005 Subject: [Histonet] separated cells Message-ID: <3.0.6.32.20031120125512.00beaa70@gemini.msu.montana.edu> >Do you mean by a perfect sheet of cells, a perfectly flat paraffin section? When you speak of sheet of cells, I envision cell cultures with a sheet of cells attached to bottom of a culture plate. > >At 12:11 PM 11/20/2003 -0500, you wrote: >>Dear Histonet, >> >>My Pathologist would like to see a perfect sheet of cells. He wants the >>vacuoles and smashed sells eliminated. We have tried many different >>processing programs and have changed the temperature of the water on the >>stainer. I have sent pictures of the problems with formalin fixed skin (cut >>at 4 microns) and a bone marrow cut at 3 microns) If anyone has any >>suggestions I would be very grateful. The pictures are listed as separated >>cells 1 & 2 @ histonet pictures. >> >>Bernadette Weston, HT >>Histology Supervisor >>Barberton Citizens Hospital >>barberton OH >> >>_________________________________________________________________ >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From JCarpenter764 <@t> aol.com Thu Nov 20 15:09:39 2003 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] different types of processors Message-ID: <3d.37f637fe.2cee8793@aol.com> Would someone please explain the difference between an open processor and a closed processor.... -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/cd37f554/attachment.htm From tissuearray <@t> hotmail.com Thu Nov 20 15:26:41 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Re: Manual embedding of tissue cores for arrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/186e189c/attachment.htm From vbaker60 <@t> yahoo.com Thu Nov 20 15:34:27 2003 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] different types of processors In-Reply-To: <3d.37f637fe.2cee8793@aol.com> Message-ID: <20031120213427.96814.qmail@web12107.mail.yahoo.com> In an open processor the tissue basket moves and the reagents are stationary (the old Autotechnicon or the Ultra Technicon). A closed processor is where the tissue is placed in a sealed tank or reservoir and the reagents are pumped in to them. (VIP, or the like). Sheehan's book "Theory & Practice of Histotechnology" has a whole chapter dedicated to this. Vikki Baker Institute for Cancer Prevention Valhalla, New York > Would someone please explain the difference between > an open processor and a > closed processor.... > __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From mari.ann.mailhiot <@t> leica-microsystems.com Thu Nov 20 16:00:26 2003 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] different types of processors Message-ID: In an open processor tissues in a basket are transferred from one solution to the next. This is sometimes referred to as the DIP and Dunk type processor. In an enclosed system, the tissue is stationary and the fluids are pumped in and out of the closed chamber holding the tissue. This system is much better for your health. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com JCarpenter764@aol.com Sent by: To: histonet@lists.utsouthwestern.edu histonet-admin@lists.utsouth cc: western.edu Subject: [Histonet] different types of processors 11/20/2003 03:09 PM Would someone please explain the difference between an open processor and a closed processor.... ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ From haldana <@t> unimoron.edu.ar Thu Nov 20 15:52:17 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Temp Agencies in Dallas area References: <20031120.095816.24069.222881@webmail15.nyc.untd.com> Message-ID: <000e01c3afb0$92141380$7504a8c0@um.edu> Help Can someone send me the exactly preparation of TBS used in immunohiatochemistry I have 3 different forms? Wich is the best? Thanks Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: To: Sent: Thursday, November 20, 2003 2:58 PM Subject: [Histonet] Temp Agencies in Dallas area > > Histonetters, > Are there any Temp Staffing agencies that cover the Dallas area only? > > Thank you. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JCarpenter764 <@t> aol.com Thu Nov 20 15:51:58 2003 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] thank you everyone, you guys are great help!!! Jennell Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/d6c989b7/attachment.htm From cwscouten <@t> myneurolab.com Thu Nov 20 16:41:17 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] different types of processors Message-ID: The closed systems are only better for your health if the Dip And Dunk system does not have enclosed area over the wells, and a charcoal filtered exhaust. Medite Processors are Dip and Dunk, and have time advantages because of that, but the systems are enclosed, and charcoal filtered air exhaust. There is no health difference. Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: mari.ann.mailhiot@leica-microsystems.com [mailto:mari.ann.mailhiot@leica-microsystems.com] Sent: Thursday, November 20, 2003 4:00 PM To: JCarpenter764@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] different types of processors In an open processor tissues in a basket are transferred from one solution to the next. This is sometimes referred to as the DIP and Dunk type processor. In an enclosed system, the tissue is stationary and the fluids are pumped in and out of the closed chamber holding the tissue. This system is much better for your health. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com JCarpenter764@aol.com Sent by: To: histonet@lists.utsouthwestern.edu histonet-admin@lists.utsouth cc: western.edu Subject: [Histonet] different types of processors 11/20/2003 03:09 PM Would someone please explain the difference between an open processor and a closed processor.... ________________________________________________________________________ This email has been scanned for all viruses by the MessageLabs Email Security System. For more information on a proactive email security service working around the clock, around the globe, visit http://www.messagelabs.com ________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Thu Nov 20 16:55:40 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] different types of processors Message-ID: There are many real advantages to a dip and move system (OPEN) when compared to a sealed flush (CLOSED) system. The following points highlight the operational differences 1) Time - the minimum cycle time for a flush system is 2 hours. This is due to the heating phase for the paraffin and the additional rinse cycles. For quick procedures such as biopsies, 2 hours is an inconvenient cycle time when it can be done in 30 minutes with a TPC system. A procedure that would take only 8 hours with the TPC will take 12 hours in a sealed system. 2) Artifacts - In order to introduce the paraffin in a sealed system, the chamber must be heated. This heating process can cause dead spots, shadows, or artifacts in your tissue. The TPC system does not create such artifacts. 3) Maintenance - The TPC system does not rely on pumps to complete the protocols. The sealed system requires pumps which can fail. These can be costly and inconvenient to replace. 4) Contamination - Although the sealed systems are thought to be contamination free, they do require cleaning after every protocol. Proteins are routinely washed out of sections during processing. These proteins will settle in the bottom of flush storage tanks and will be present for future protocols. In order to remove all contaminates, the system must be cleaned. 5) Cost - The TPC system uses a much lower volume of reagents and chemicals. These add up over the coarse of a year. Have you actually calculated your annual costs and looked at the potential savings? 6) Tissue Shrinking - The TPC system does not expose the tissue to a heating cycle and therefore does not cause the tissue to shrink. I am also attaching a detailed data sheet here. If you have any questions or would like to discuss this further please feel free to contact me anytime. Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Thursday, November 20, 2003 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] different types of processors Would someone please explain the difference between an open processor and a closed processor.... -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/6b8f854e/attachment.htm From AnthonyH <@t> chw.edu.au Thu Nov 20 17:35:39 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] different types of processors Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0B2@simba.kids> I have used both types and agree with the comments made by Charles. Some further points: 1. Closed systems process large blocks (eg brain) and fatty tissues better than open systems due to the stronger pressure and vacuum that can be applied. 2. To obtain equivalent processing quality from an open system requires at least double the processing time. 3. Because open systems are more gentle on tissues, small endoscopics and core biopsies process better than with closed systems. 4. The choice? For large throughput, general path labs definitely consider at least one closed system processor and for Children's Hospital labs (smaller biopsies), open system processors are definitely wothwhile. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Charles W. Scouten, Ph.D. [mailto:cwscouten@myneurolab.com] Sent: Friday, 21 November 2003 9:56 AM To: JCarpenter764@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] different types of processors There are many real advantages to a dip and move system (OPEN) when compared to a sealed flush (CLOSED) system. The following points highlight the operational differences 1) Time - the minimum cycle time for a flush system is 2 hours. This is due to the heating phase for the paraffin and the additional rinse cycles. For quick procedures such as biopsies, 2 hours is an inconvenient cycle time when it can be done in 30 minutes with a TPC system. A procedure that would take only 8 hours with the TPC will take 12 hours in a sealed system. 2) Artifacts - In order to introduce the paraffin in a sealed system, the chamber must be heated. This heating process can cause dead spots, shadows, or artifacts in your tissue. The TPC system does not create such artifacts. 3) Maintenance - The TPC system does not rely on pumps to complete the protocols. The sealed system requires pumps which can fail. These can be costly and inconvenient to replace. 4) Contamination - Although the sealed systems are thought to be contamination free, they do require cleaning after every protocol. Proteins are routinely washed out of sections during processing. These proteins will settle in the bottom of flush storage tanks and will be present for future protocols. In order to remove all contaminates, the system must be cleaned. 5) Cost - The TPC system uses a much lower volume of reagents and chemicals. These add up over the coarse of a year. Have you actually calculated your annual costs and looked at the potential savings? 6) Tissue Shrinking - The TPC system does not expose the tissue to a heating cycle and therefore does not cause the tissue to shrink. I am also attaching a detailed data sheet here. If you have any questions or would like to discuss this further please feel free to contact me anytime. Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] Sent: Thursday, November 20, 2003 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] different types of processors Would someone please explain the difference between an open processor and a closed processor.... ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/62301cfa/attachment.htm From mikesafron <@t> copper.net Thu Nov 20 18:01:32 2003 From: mikesafron <@t> copper.net (mikesafron) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: <173690-22003115210132494@copper.net> You are correct. You need to have your 2 years in prior to the last registration date of 2004. Which I think is in September. Michael Safron A.S., HT(ASCP) Manager, Histology WIL Research Laboratories Inc. ---- Original Message ---- From: FreidaC@aol.com To: GREYTRUNK@aol.com, histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech Date: Thu, 20 Nov 2003 10:09:40 EST >I do not believe it is correct that you have until December 31, 2004 >to sign >up for the ASCP certifying exam. Without an associate degree, you >must TAKE >exam for the first time before December 31, 2004. This obviously >means that >you must register for it much earlier. > >Freida Carson > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From teresajharris <@t> msn.com Sat Nov 15 11:06:34 2003 From: teresajharris <@t> msn.com (teresajharris@msn.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Exhaust Block References: Message-ID: It is a pleasure to hear what other histotech's are asked to do by the pathologist. I, too, am asked to exhaust the block. After I exhaust the block, it gets sent out for a second opinion. I really don't know the reasons why, just know what I am asked to do. ----- Original Message ----- From: Deltour, Douglas D.(HM2) To: 'Cindy DuBois' ; histonet@lists.utsouthwestern.edu Sent: Friday, November 14, 2003 11:49 PM Subject: RE: [Histonet] Exhaust Block It sounds like he is doing the overkill CYA thing. Was he ever a Navy Pathologist? HM2(FMF) Douglas D. Deltour Naval Hospital Sigonella Italy LPO Histology Histology Technician DSN 624-4669 FAX 624-4680 COMM (From US) 011-39-095-56-4669 DISCLAIMER: Confidentiality Notice - This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. **** Informazione Confidenziale - Questa e-Mail, incluso eventuali allegati, contiene informazioni confidenziali intese unicamente alla persona/e a cui e' indirizzata. Se avete ricevuto per errore questo messaggio, cortesemente contattare immediatamente il mittente e cancellare la e-Mail. Grazie****. -----Original Message----- From: Cindy DuBois [mailto:ccdub@earthlink.net] Sent: Friday, November 14, 2003 8:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exhaust Block We have a pathologist who recently decided that if the specimen doesn't show a disease process (ie, no pathological abnormality) we must "exhaust the block". This means (to him) to make 3 additional levels (H&E's), then cut through the rest of the tissue leaving nothing in the block. We are suppose to do this to keep the patient or referring physician from requesting further testing on the block. So far we only have to do this with skin biopsies, but I am afraid he will soon want it for everything else. This is extremely time consuming! Do other labs out there do this? I am just curious. Cindy DuBois, HT ASCP Delta Pathology Assoc. Stockton, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031115/59e973a9/attachment.htm From RSRICHMOND <@t> aol.com Thu Nov 20 22:17:41 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] RE: embedding like specimens sequentially Message-ID: <14c.2713763f.2ceeebe5@aol.com> Sheila Tapper recently submitted a query - to which several people replied - about whether a surgical pathology service should avoid accessioning grossly similar specimens (such as prostate needle biopsies) sequentially rather than spacing them with histologically dissimilar specimens. I certainly concur with Curtis Tague: >>I'd have your pathologist put the hammer down. He or she would no doubt be happy to see your desire to improve quality control and reduce the chance any mix up that could lead to legal problems. Explain to them the risks and I'm sure they'll back you.<< As a surgical pathologist with experience in about 60 surgical pathology services over the last 40 years, I'd say that avoiding sequential accessioning of similar specimens is standard practice - I learned it in residency and have seen many services apply it since then. I see no reason to have to cite "chapter and verse" - but as a matter of fact the College of American Pathologists is considering a resolution on this and related topics right now. My friend and former locum tenens client Joseph C. Bergeron Jr. MD FCAP is now chairing the CAP's House Ad Hoc Committee on Medicolegal Testimony.He's submitted CAP House of Delegates Resolution F2003-55: "Guidelines regarding the technical handling and reporting aspects of histology" - which is somewhere in process right now, and is still in draft form. Perhaps one of the standard histotechnology references cites this practice - Freida Carson, do you know of such a citation? If you're writing a procedure, you need to make clear that this is a rule that can be broken - if three prostate biopsy specimens come in in the late afternoon and you have no other specimens to space them with, then you accession them sequentially. I have to be vague about the details, but right now I'm serving as an expert witness in a case where a disastrous outcome occurred as a result of a mixup of adjacent similar specimens. Sequence of accessioning is one of the issues that's been raised in depositions. Since this note is going into the HistoNet archives, you can cite it as a reference! Bob Richmond Samurai Pathologist Knoxville TN (Robert S. Richmond, MD, FCAP) -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/a0419830/attachment.htm From RSRICHMOND <@t> aol.com Thu Nov 20 22:17:54 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Re: HELICOBACTER CONTROLS Message-ID: <9.1cd16f68.2ceeebf2@aol.com> Tasha R. Bourm at Olympic Medical Center in Port Angeles WA (up at the far end of the Olympic Peninsula - wotta view!) asks about Helicobacter pylori controls for staining gastric biopsy specimens, and several people replied. The varied pathology services I've done locum tenens work for have real difficulty getting Helicobacter controls, mostly because the histotechnologists aren't communicating with the pathologists and the transcriptionists. When I see a case that would make a good Helicobacter control, I send in a special stain request form with "good Helicobacter control" scribbled on it instead of a request for a stain. The ideal control tissue is a gastrectomy specimen crawling with bugs. Fortunately for patients, unfortunately for pathology services, such specimens have become rare. But look for these specimens; one good case can supply you with controls from now until your Social Security kicks in. Helicobacter pylori is extremely difficult to culture - most small labs have never succeeded - so that cultures are an unlikely source of controls. I really don't like to see other bacteria used as controls. Many services either don't do a Helicobacter control, or use some irrelevant piece of tissue - and all the commercial controls I've seen have been worthless. I don't think this is acceptable practice - though I don't think it's necessary to run a positive control with every Giemsa stain run (immune stains do of course require a positive control). I've become convinced that it's necessary to use an oil immersion lens with Giemsa stains for Helicobacter, a view that makes me unpopular with my fellow pathologists. One of the advantages of the immune stain, in my personal experience, is that it allows you dispense with oil immersion, a time-consuming procedure. Bob Richmond Samurai Pathologist Knoxville TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031120/636cf633/attachment.htm From Nancy.Walker <@t> sanofi-synthelabo.com Fri Nov 21 03:24:54 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] processing adipose tissue Message-ID: Bonjour, I have adipose tissue to process (fix and paraffin embedding) for in situ hybridization (ISH) and IHC experiments. I've done this in the past with a program and got pretty good tissue morphology and done some experiments, but since I was told by Gayle Callis that my processing schedules for rodent brain and embryo are probably excessively long. In fact in a recent experiment where I included an embryo slide prepared by Novagen; Signals were much better with the commercially supplied slide. So does anyone have any advice on processing fat? I'm using small samples of epididymal fat (0.5g - 1 g per sample ) that have been fixed overnight in PFA 4% and washed in PBS for 6h. This is the program we used : alcohol 70% 1h 95% 2X1h 100% 2X1h 100% 2h clearify 3X1h paraffine 3X1h30 (Sakura tissue tech III) (Our embedding machine does have possibilities to do vacuum and heat). Before doing IHC or ISH we just deparaffin with xylene (2x5') and rehydrate. We do Protein K before in situ and microwave or trypsin or EDTA before IHC . Are there some specific pretreatments for adipose tissue for delipidizing that might improve probe or antibody accessibility. I appreciate your advice and thanks for your valuable time, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From lynzjackson <@t> hotmail.com Fri Nov 21 04:13:46 2003 From: lynzjackson <@t> hotmail.com (lynsay jackson) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] erbB3 and erbB4 Immunohistochemistry Message-ID: Hi, I am interested in carrying out research use erbB3 and erbB4 antibodies on rat and mouse xenograft material using Immunohistochemistry. Has anyone had any success with any antibodies for these targets, and if so which companies would you recommend to buy the antibodies from. _________________________________________________________________ Use MSN Messenger to send music and pics to your friends http://www.msn.co.uk/messenger From tissuearray <@t> hotmail.com Fri Nov 21 04:38:56 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Manual array blocks - Materials Information Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/ed6eae92/attachment.htm From lpwenk <@t> covad.net Fri Nov 21 05:05:24 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech References: <002c01c3af85$8f786890$7300000a@histo2> Message-ID: <00b401c3b01f$5d2841e0$8732fea9@hppav> One (long) added comment to Peggy's and Frieda's comments. A candidate must TAKE the HT exam before Jan. 2005. However, they do not have to PASS the exam before that deadline. When someone signs up to take the HT exam (or other ASCP certification exams), they are given a 5 year time frame. Also, they have 5 times in which to take the exam. EXAMPLE: If the person takes the HT exam for the first time in April 2004 (second cycle), and does not pass it, and then tries again in Oct. 2004 (4th cycle), and still does not pass it, they still have 3 more times to take and pass the exam. Since they first took the exam in the 2nd cycle of 2004, they would have 2005, 2006, 2007, 2009 (just Jan -Mar 2009 = first cycle) in which to pass in one of their last 3 tries. Now remember, they have to pass both the written and practical exam. But not necessarily at the same time. EXAMPLE: If the person passes the practical on the first try, but not the written, they just have to repeat the written. Their passing practical grade is good for that 5 year period. Whenever they pass the written (say, the 3rd time), the two passing scores are combined, and they have now passed the HT certification exam. If someone who qualifies through Route 3 (high school diploma + 2 years OJT), but does not take the exam for the first time before Jan. 2005, they can still go on and earn their associate degree with 20 hours biology/chemistry, and then take the HT exam in the future via the associate degree route (Route 2). Or, if they can get into a NAACLS-accredited HT program, they can still take the HT exam in the future via that route (Route 1). However, if someone trying via the high school diploma route uses up all of their 5 chances over the next 5 years, and then in the future earns their associate degree with the required bio/chem, they would NOT be allowed to retake the HT certification exam. A candidate is given a total of 5 times to take a specific certification exam, regardless of how many different routes through which they try. If, on the other hand, they could continue with their education, earn their baccalaureate degree with 30 hours of bio/chem, they can now take the HTL exam, and be given 5 new chances. They are now taking a DIFFERENT certification exam. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Peggy Micciche" To: Sent: Thursday, November 20, 2003 11:44 AM Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech > You must apply by July 1, 2004 to be eligible to take the exam via Route 3 > which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at > www.ascp.org for more information. > > Peggy Micciche > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > FreidaC@aol.com > Sent: Thursday, November 20, 2003 10:10 AM > To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech > > > I do not believe it is correct that you have until December 31, 2004 to sign > up for the ASCP certifying exam. Without an associate degree, you must TAKE > exam for the first time before December 31, 2004. This obviously means that > you must register for it much earlier. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From georgecole <@t> ev1.net Fri Nov 21 05:20:12 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Avoinding confusion of cases Message-ID: <000001c3b021$6f18ee20$044dbad0@hppav> Bob Richman has a couple of interesting messages in the histonet----about not doing similar cases in a row, to avoid mix ups with the resulting expensive litigation. Now I'm the one who's sending the muscle and nerve packets all over the place. I did nothing but same cases in the 6,359 muscles and 2000plus nerves. There was rarely any "other" to process in between my specialty. I didn't even think of this when I gave each biopsy my own number. My muscles were numbered from 1 to 6,359. I sent the reports and case paper work to the main lab. They assigned a number to the case. But all my slides clear through prelim H & E's to histochemistry had my number on it. When a case was ready to go to the neuropthologist, I put labels on the slides with the department accession number on them.. At any rate my method of numbering the cases worked very well. I think the trouble you discuss is more likely to occur when you use the official surgical numbers, which are assigned by someone else and get to be large soon in the year. The one who cuts in marks a strictly nominal number on the paraffin mold. But my numbers were real sequence numbers with no letters in it that I gave to the case and wrote it on its mold and in my register. But every slide of mine was numbered in sequence with that number in indelible ink from the start to keep things straight until the accession number came out. When that happened, I labeled the slides and put the official accession number on the labels. Of course, this procedure would not work in the main lab. But when more than one specimen of the same tissue comes in, in a row, isn't there an added code digit used, added to the accession number, to indicate like specimens, and to keep each of the same tissue cases in sequence??---This must alert the histotechs to the problem and keep these cases straight. When the histotech starts to cut, it would be the first thing to do to sort the blocks, and spot the same-tissue cases and identify them on their slides, and to alert them to the need for extra care in sectioning, marking the slides, and avoiding mix ups. .georgecole@ev1.net . -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/a74f254d/attachment.htm From Hedley.Glencross <@t> CMMC.nhs.uk Fri Nov 21 05:55:21 2003 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] fussin & feudin Message-ID: Hi Sarah Hopefully I'm now in plain text. I was just wondering if you thought we here in the UK might be "whoopin & a'hollerin" at the visit of your leader. Hedley Glencross Manchester Cytology Centre UK Message: 2 Date: Wed, 19 Nov 2003 17:27:01 -0600 From: "Sarah Jones" To: , Cc: Subject: RE: [Histonet] embedding like specimens sequentially This is an intriguing problem. I'm unsure why anyone would court disaster if it could be avoided. I checked the web page http://www.pathologistsassistants.org/ PA's do work under the direction of the pathologist so as Curtis pointed out, that would be one way to handle it. Also, the web site states under the code of regulation: Section 3. Service. A Pathologists' Assistant is qualified by academic and practical training to provide the following services under the direction and supervision of a pathologist: A. Preparation, gross description and dissection of human tissue surgical specimens including: 1. Assuring appropriate specimen accessioning. Since this is a bit ambiguous, it could be put in your procedure manual as an SOP and then all the fussin an feudin would be put to an end. I'm puttin that "fussin and feudin" lingo in their for all our UK friends that have been so privileged to hear our fearless leader (cough, cough) speak this week. He's very brave when it comes to bombing and sending the troops in, but Members of parliament are just too much for him! ;) Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From Nancy.Walker <@t> sanofi-synthelabo.com Fri Nov 21 06:27:10 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] =?iso-8859-1?Q?R=E9f=2E_=3A_RE=3A_[Histonet]_processing_adipose_tissue?= Message-ID: Hello, I'm not really interested in the lipids but rather the proteins and mRNA of adipocytes and/or stromal tissue. The most basic question is whether the target gene is expressed in the stoma or by the adipocyte. If I'm using in situ hybridisation the mRNA is found in the cytoplasm not in the lipid droplet, which indeed will be removed by the paraffination et deparafination. I've been told that frozen tissue often needs to be delipidized to render the mRNA more accessible to hybridization. In the case of IHC the paraffin tissue also has the same advantage (better accessibility, better cellular morphology - particulary if one is trying to differentiate mulitlocular adipocytes, histiocytes, blood vessels). Only for proteins secreted by the adipocyte and stored in the vacuole would paraffin slices be not the best bet. However even in some cases of secreted proteins like ACRP, some protein can be detected on the cytoplasmic rim of a adipocyte even after parrafination. correct me if I'm wrong or imprecise, yours truely, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 "George Cole" cc : Objet : RE: [Histonet] processing adipose tissue 21/11/03 12:31 Nancy, No advice here, but questions:---doesn't the clearing process in paraffin impregnation interfere with the nature of adipose tissue? It can't really tell you anything about that which was melted away in the clearing step. Are frozen sections useful for your work? Can you not get sections that are useful to you with lipids cut in a good cryostat at -25 to -30C on a very sharp knife? georgecole@ev1.net -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Nancy.Walker@sanofi-synthelabo.com Sent: Friday, November 21, 2003 1:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing adipose tissue Bonjour, I have adipose tissue to process (fix and paraffin embedding) for in situ hybridization (ISH) and IHC experiments. I've done this in the past with a program and got pretty good tissue morphology and done some experiments, but since I was told by Gayle Callis that my processing schedules for rodent brain and embryo are probably excessively long. In fact in a recent experiment where I included an embryo slide prepared by Novagen; Signals were much better with the commercially supplied slide. So does anyone have any advice on processing fat? I'm using small samples of epididymal fat (0.5g - 1 g per sample ) that have been fixed overnight in PFA 4% and washed in PBS for 6h. This is the program we used : alcohol 70% 1h 95% 2X1h 100% 2X1h 100% 2h clearify 3X1h paraffine 3X1h30 (Sakura tissue tech III) (Our embedding machine does have possibilities to do vacuum and heat). Before doing IHC or ISH we just deparaffin with xylene (2x5') and rehydrate. We do Protein K before in situ and microwave or trypsin or EDTA before IHC . Are there some specific pretreatments for adipose tissue for delipidizing that might improve probe or antibody accessibility. I appreciate your advice and thanks for your valuable time, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Nov 21 06:33:36 2003 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] re erb3 and 4 References: <20031121115800.5701.85546.Mailman@swlx167.swmed.edu> Message-ID: <008701c3b02b$af033090$e8345c9f@Carlos> I've recently used santa cruz erbB3( sc-285) and erbB4( sc-283) on mouse pwax sections. Had to use TRIS pH10 AR to get them to work. 4 works very well, 3 is possibly dodgy...but I'm using neuronal tissue and I don't know if 3's strong there anyway.They work fine on frozens too, if I remember From Delventh3 <@t> aol.com Fri Nov 21 06:50:17 2003 From: Delventh3 <@t> aol.com (Delventh3@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] online college courses Message-ID: <94.4019e950.2cef6409@aol.com> Hello histonetters-I came across this URL for online college courses. It looked like something helpful for those of you struggling to balance careers, family and further education. http://www.coursepal.com/apps/go.php4?id=IKZ Priscilla in Central Wyoming (currently working in New Mexico) -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/61f1ba09/attachment.htm From tissuearray <@t> hotmail.com Fri Nov 21 07:27:50 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Dermal Needles for Arrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/9e208664/attachment.htm From tissuearray <@t> hotmail.com Fri Nov 21 08:04:05 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Dermal Needles for Arrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/7a45eca5/attachment.htm From lchung <@t> ppmh.org Fri Nov 21 07:39:09 2003 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again? Just a thought. Bruce Chung Anatomic Pathology Manager -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Friday, November 21, 2003 6:05 AM To: Peggy Micciche; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech One (long) added comment to Peggy's and Frieda's comments. A candidate must TAKE the HT exam before Jan. 2005. However, they do not have to PASS the exam before that deadline. When someone signs up to take the HT exam (or other ASCP certification exams), they are given a 5 year time frame. Also, they have 5 times in which to take the exam. EXAMPLE: If the person takes the HT exam for the first time in April 2004 (second cycle), and does not pass it, and then tries again in Oct. 2004 (4th cycle), and still does not pass it, they still have 3 more times to take and pass the exam. Since they first took the exam in the 2nd cycle of 2004, they would have 2005, 2006, 2007, 2009 (just Jan -Mar 2009 = first cycle) in which to pass in one of their last 3 tries. Now remember, they have to pass both the written and practical exam. But not necessarily at the same time. EXAMPLE: If the person passes the practical on the first try, but not the written, they just have to repeat the written. Their passing practical grade is good for that 5 year period. Whenever they pass the written (say, the 3rd time), the two passing scores are combined, and they have now passed the HT certification exam. If someone who qualifies through Route 3 (high school diploma + 2 years OJT), but does not take the exam for the first time before Jan. 2005, they can still go on and earn their associate degree with 20 hours biology/chemistry, and then take the HT exam in the future via the associate degree route (Route 2). Or, if they can get into a NAACLS-accredited HT program, they can still take the HT exam in the future via that route (Route 1). However, if someone trying via the high school diploma route uses up all of their 5 chances over the next 5 years, and then in the future earns their associate degree with the required bio/chem, they would NOT be allowed to retake the HT certification exam. A candidate is given a total of 5 times to take a specific certification exam, regardless of how many different routes through which they try. If, on the other hand, they could continue with their education, earn their baccalaureate degree with 30 hours of bio/chem, they can now take the HTL exam, and be given 5 new chances. They are now taking a DIFFERENT certification exam. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Peggy Micciche" To: Sent: Thursday, November 20, 2003 11:44 AM Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech > You must apply by July 1, 2004 to be eligible to take the exam via Route 3 > which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at > www.ascp.org for more information. > > Peggy Micciche > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > FreidaC@aol.com > Sent: Thursday, November 20, 2003 10:10 AM > To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech > > > I do not believe it is correct that you have until December 31, 2004 to sign > up for the ASCP certifying exam. Without an associate degree, you must TAKE > exam for the first time before December 31, 2004. This obviously means that > you must register for it much earlier. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. From Liam.Brennan <@t> bll.n-i.nhs.uk Fri Nov 21 08:09:50 2003 From: Liam.Brennan <@t> bll.n-i.nhs.uk (Brennan, Liam) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Dermal Needles for Arrays Message-ID: Thom- What about using a bone marrow trephine needle, as far as I know they come in a variety of diameters and come with a stylet. They are disposable, not prohibitively expensive, and could be discarded when they become blunt. Liam Brennan Histopathology Dept Belfast City Hospital Northern Ireland -----Original Message----- From: Thom Jensen [mailto:tissuearray@hotmail.com] Sent: 21 November 2003 13:28 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dermal Needles for Arrays The company I posted to purchase Dermal needles has apparently gone out of business. The link below is where I found the needles. The reason why I picked this particular needle is because the middle of the middle goes all the way though for a stylet to be used. Some needles on the market have solid plastic handles and not useful for array punching. http://www.steeles.com/temp/search.php?input=33-31 &choice=2&submitfind=Search Thom Jensen _____ Has one of the new viruses infected your computer? Find out with a FREE online computer virus scan from McAfee. Take the FreeScan now! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Fri Nov 21 08:50:16 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Biotin block after primary? Message-ID: You betcha! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: brade, brade upland, upland [mailto:newhistotech@yahoo.com] Sent: Wednesday, November 19, 2003 9:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biotin block after primary? Hi, I accidently added my primary antibody before avidin/biotin blocking. Is it possible to block after the primary but before a biontinylated secondary? Thank you for help. _____ Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/66a0859c/attachment.htm From RSRICHMOND <@t> aol.com Fri Nov 21 10:16:41 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Re: Exhaust Block Message-ID: <11a.2b4954e1.2cef9469@aol.com> Cindy Dubois (Stockton CA) and Douglas D. Deltour (at the US naval hospital in Sigonella, Italy) comment on a pathologist's practice of having a block destroyed by cutting it down, in order to keep anyone else from doing additional studies on it. I've never heard of such a practice, in a great number of pathology laboratories. It's irresponsible patient care, and, far from being a CYA, leaves the pathologist open to litigation, simply because it isn't anybody's standard of care. I frequently have a lot of difficulty - when I'm new on a pathology service - in communicating to histotechnologists just what I want them to do to a block. Usually I go get the paraffin block myself, to look at it and see whether it's already been exhausted, or whether it's been inadequately cut into. Sometimes I want a block cut with minimal trimming - say if I'm looking at a small focus of cancer in a prostate biopsy specimen - and sometimes I want it exhausted, say if I'm looking for a cervical dysplasia that probably isn't there. Requests like "please recut without trimming" or "please cut through the block x3 or more" often aren't understood. In particular, many histotechnologists are reluctant to exhaust a block, even when the pathologist specifically requests it. There is no reason to send an obviously exhausted block to a consultant. The pathologist's covering letter (he DOES write a covering letter, doesn't she?) should state that the block has been exhausted. [I apologize for sending this letter HTML formatted - the present version of AOL's mail client requires it. I look forward to the coming update of this listserv that will strip HTML from posts.] Bob Richmond Samurai Pathologist Knoxville TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/0261fd0e/attachment.htm From amosbrooks <@t> earthlink.net Fri Nov 21 10:20:36 2003 From: amosbrooks <@t> earthlink.net (amosbrooks) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Political histology Message-ID: <9256300.1069431636640.JavaMail.Administrator@ATP2> Why, Did he have a biopsy done there? Or did he mention histology while there? I don't recall Mr. Bush's visit having anything to do with histology or ehen healthcare at all. I think I must have missed something. Hi Sarah Hopefully I'm now in plain text. I was just wondering if you thought we here in the UK might be "whoopin = & a'hollerin" at the visit of your leader. Hedley Glencross Manchester Cytology Centre UK From DRG <@t> Stowers-Institute.org Fri Nov 21 10:27:27 2003 From: DRG <@t> Stowers-Institute.org (Grant, Debra) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Small paraffin dispenser Message-ID: Hi Histonetters, We are looking for a small (1 liter) paraffin dispenser to keep harder paraffin for bone in. Your help is appreciated, Thanks! Debby Grant Research Technician II Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org From tpmorken <@t> labvision.com Fri Nov 21 10:34:42 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become His totech Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD5A@usca0082k03.rallansci.apogent.com> Bruce wrote: << What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again?> Brue, sorry to say, that's right, You can't be a certified histotech. Your dreams are shattered and you will be forced to become a pathologist if you want to keep working in the field. Tim Morken -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Friday, November 21, 2003 5:39 AM To: 'Lee & Peggy Wenk'; Histonet (E-mail) Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again? Just a thought. Bruce Chung Anatomic Pathology Manager -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Friday, November 21, 2003 6:05 AM To: Peggy Micciche; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech One (long) added comment to Peggy's and Frieda's comments. A candidate must TAKE the HT exam before Jan. 2005. However, they do not have to PASS the exam before that deadline. When someone signs up to take the HT exam (or other ASCP certification exams), they are given a 5 year time frame. Also, they have 5 times in which to take the exam. EXAMPLE: If the person takes the HT exam for the first time in April 2004 (second cycle), and does not pass it, and then tries again in Oct. 2004 (4th cycle), and still does not pass it, they still have 3 more times to take and pass the exam. Since they first took the exam in the 2nd cycle of 2004, they would have 2005, 2006, 2007, 2009 (just Jan -Mar 2009 = first cycle) in which to pass in one of their last 3 tries. Now remember, they have to pass both the written and practical exam. But not necessarily at the same time. EXAMPLE: If the person passes the practical on the first try, but not the written, they just have to repeat the written. Their passing practical grade is good for that 5 year period. Whenever they pass the written (say, the 3rd time), the two passing scores are combined, and they have now passed the HT certification exam. If someone who qualifies through Route 3 (high school diploma + 2 years OJT), but does not take the exam for the first time before Jan. 2005, they can still go on and earn their associate degree with 20 hours biology/chemistry, and then take the HT exam in the future via the associate degree route (Route 2). Or, if they can get into a NAACLS-accredited HT program, they can still take the HT exam in the future via that route (Route 1). However, if someone trying via the high school diploma route uses up all of their 5 chances over the next 5 years, and then in the future earns their associate degree with the required bio/chem, they would NOT be allowed to retake the HT certification exam. A candidate is given a total of 5 times to take a specific certification exam, regardless of how many different routes through which they try. If, on the other hand, they could continue with their education, earn their baccalaureate degree with 30 hours of bio/chem, they can now take the HTL exam, and be given 5 new chances. They are now taking a DIFFERENT certification exam. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Peggy Micciche" To: Sent: Thursday, November 20, 2003 11:44 AM Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech > You must apply by July 1, 2004 to be eligible to take the exam via Route 3 > which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at > www.ascp.org for more information. > > Peggy Micciche > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > FreidaC@aol.com > Sent: Thursday, November 20, 2003 10:10 AM > To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech > > > I do not believe it is correct that you have until December 31, 2004 to sign > up for the ASCP certifying exam. Without an associate degree, you must TAKE > exam for the first time before December 31, 2004. This obviously means that > you must register for it much earlier. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 21 10:50:35 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: :-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: 21 November 2003 16:35 To: Histonet (E-mail) Subject: RE: 5-time limit. [Histonet] Deadline for 2 yr OJT to become Histotech Bruce wrote: << What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again?> Brue, sorry to say, that's right, You can't be a certified histotech. Your dreams are shattered and you will be forced to become a pathologist if you want to keep working in the field. Tim Morken -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Friday, November 21, 2003 5:39 AM To: 'Lee & Peggy Wenk'; Histonet (E-mail) Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again? Just a thought. Bruce Chung Anatomic Pathology Manager -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Friday, November 21, 2003 6:05 AM To: Peggy Micciche; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech One (long) added comment to Peggy's and Frieda's comments. A candidate must TAKE the HT exam before Jan. 2005. However, they do not have to PASS the exam before that deadline. When someone signs up to take the HT exam (or other ASCP certification exams), they are given a 5 year time frame. Also, they have 5 times in which to take the exam. EXAMPLE: If the person takes the HT exam for the first time in April 2004 (second cycle), and does not pass it, and then tries again in Oct. 2004 (4th cycle), and still does not pass it, they still have 3 more times to take and pass the exam. Since they first took the exam in the 2nd cycle of 2004, they would have 2005, 2006, 2007, 2009 (just Jan -Mar 2009 = first cycle) in which to pass in one of their last 3 tries. Now remember, they have to pass both the written and practical exam. But not necessarily at the same time. EXAMPLE: If the person passes the practical on the first try, but not the written, they just have to repeat the written. Their passing practical grade is good for that 5 year period. Whenever they pass the written (say, the 3rd time), the two passing scores are combined, and they have now passed the HT certification exam. If someone who qualifies through Route 3 (high school diploma + 2 years OJT), but does not take the exam for the first time before Jan. 2005, they can still go on and earn their associate degree with 20 hours biology/chemistry, and then take the HT exam in the future via the associate degree route (Route 2). Or, if they can get into a NAACLS-accredited HT program, they can still take the HT exam in the future via that route (Route 1). However, if someone trying via the high school diploma route uses up all of their 5 chances over the next 5 years, and then in the future earns their associate degree with the required bio/chem, they would NOT be allowed to retake the HT certification exam. A candidate is given a total of 5 times to take a specific certification exam, regardless of how many different routes through which they try. If, on the other hand, they could continue with their education, earn their baccalaureate degree with 30 hours of bio/chem, they can now take the HTL exam, and be given 5 new chances. They are now taking a DIFFERENT certification exam. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Peggy Micciche" To: Sent: Thursday, November 20, 2003 11:44 AM Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech > You must apply by July 1, 2004 to be eligible to take the exam via Route 3 > which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at > www.ascp.org for more information. > > Peggy Micciche > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > FreidaC@aol.com > Sent: Thursday, November 20, 2003 10:10 AM > To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech > > > I do not believe it is correct that you have until December 31, 2004 to sign > up for the ASCP certifying exam. Without an associate degree, you must TAKE > exam for the first time before December 31, 2004. This obviously means that > you must register for it much earlier. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nick.kirk3 <@t> btopenworld.com Fri Nov 21 11:01:21 2003 From: nick.kirk3 <@t> btopenworld.com (nick.kirk3@btopenworld.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Political histology Message-ID: <4179902.1069434081548.JavaMail.root@127.0.0.1> Don't know about that but he had fish, chips and mushy peas for his pub lunch today, so there may well be a tail wind behind Air Force One going back to the US tonight. (For our American Cousins who aren't aware of what mushy peas are, they are an essential accompanyment to fish and chips and have a rather unfortunate side effect if you're not used to them! Think of the camp fire scene in Blazing Saddles and you'll get the idea) Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon (3 miles from a US airbase so I had better watch what I say) England > from: amosbrooks > date: Fri, 21 Nov 2003 16:20:36 > to: histonet@lists.utsouthwestern.edu > subject: Re: [Histonet] Political histology > > Why, > Did he have a biopsy done there? Or did he mention histology while > there? I don't recall Mr. Bush's visit having anything to do with histology > or ehen healthcare at all. I think I must have missed something. > > > Hi Sarah > > Hopefully I'm now in plain text. > > I was just wondering if you thought we here in the UK might be "whoopin = > & a'hollerin" at the visit of your leader. > > Hedley Glencross > Manchester Cytology Centre UK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 21 11:09:03 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Political histology>mushy peas Message-ID: Mushy peas = peas with nuclear artefact and overstained with malachite green. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: nick.kirk3@btopenworld.com [mailto:nick.kirk3@btopenworld.com] Sent: 21 November 2003 17:01 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Political histology Don't know about that but he had fish, chips and mushy peas for his pub lunch today, so there may well be a tail wind behind Air Force One going back to the US tonight. (For our American Cousins who aren't aware of what mushy peas are, they are an essential accompanyment to fish and chips and have a rather unfortunate side effect if you're not used to them! Think of the camp fire scene in Blazing Saddles and you'll get the idea) Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon (3 miles from a US airbase so I had better watch what I say) England > from: amosbrooks > date: Fri, 21 Nov 2003 16:20:36 > to: histonet@lists.utsouthwestern.edu > subject: Re: [Histonet] Political histology > > Why, > Did he have a biopsy done there? Or did he mention histology while > there? I don't recall Mr. Bush's visit having anything to do with histology > or ehen healthcare at all. I think I must have missed something. > > > Hi Sarah > > Hopefully I'm now in plain text. > > I was just wondering if you thought we here in the UK might be "whoopin = > & a'hollerin" at the visit of your leader. > > Hedley Glencross > Manchester Cytology Centre UK > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Fri Nov 21 11:21:28 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Political histology>mushy peas Message-ID: Are the peas smashed up or what? I can't quite picture them - like baby food??? EEEEwwwww. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Fax 847.938.3266 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/a78e9236/attachment.htm From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 21 11:22:22 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Political histology>mushy peas Message-ID: Best mental picture is to imagine they have already been eaten by someone who has then changed his mind:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: 21 November 2003 17:21 To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Political histology>mushy peas Are the peas smashed up or what? I can't quite picture them - like baby food??? EEEEwwwww. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Fax 847.938.3266 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/9272130c/attachment.htm From Robert.Lott <@t> bhsala.com Fri Nov 21 11:42:05 2003 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Lab Vision's new Rabbit Monoclonal Abs Message-ID: <35B6C610DD1DD311B1FA0008C791400407F30049@gobexchm3.bhsala.com> At the NSH convention in Louisville there was a poster presentation regarding Lab Vision's new RABBIT monoclonal Ab against Estrogen Receptor (Clone SP1)... has anyone else had any experience with this clone OR their new RABBIT monoclonal Ab against CD3 (Clone SP7) or Cyclin D1/bcl-1 (Clone SP4) or Calretinin (Clone SP13)? Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/5ceb9dd5/attachment.htm From tpmorken <@t> labvision.com Fri Nov 21 12:03:03 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Political histology>mushy peas Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD5D@usca0082k03.rallansci.apogent.com> Considering that pork rinds (deep-fried pig skin) is one of Bush's favorite snacks, I'll bet the mushy peas were easy to take. Tim Morken -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, November 21, 2003 9:22 AM To: Jackie.O'Connor@abbott.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Political histology>mushy peas Best mental picture is to imagine they have already been eaten by someone who has then changed his mind:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: 21 November 2003 17:21 To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Political histology>mushy peas Are the peas smashed up or what? I can't quite picture them - like baby food??? EEEEwwwww. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Fax 847.938.3266 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/d8f260c6/attachment.htm From dmikita <@t> wmcnet.org Fri Nov 21 12:09:35 2003 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Full-time Histology Position Open Message-ID: With the great outdoors at your doorstep, no state income tax, minimal managed care, and a community suitable for raising a family, Wyoming is a great place to spread your wings. Join a hospital where you're made to feel at home and allowed to grow in your profession. We are currently seeking a Histologic Technician. Requirements include a one year certificate from college or technical school; and three to six months related experience and/or training; or equivalent combination of education and experience. Must also be ASCP registered, equivalent or eligible. We offer exceptional benefits, compensation, and relocation assistance. Please call 307-577-2406 or 800-822-7201 x 2406 for inquiries, fax your resume to 307-577-2579 or apply online at www.wmcnet.org. Wyoming Medical Center is an equal opportunity employer. From Rcartun <@t> harthosp.org Fri Nov 21 12:13:12 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Lab Vision's new Rabbit Monoclonal Abs Message-ID: Hi Robert: We are very impressed with their cyclin D1 antibody; the ER and CD3 work well, but we are happy with our current antibodies. Rich Cartun >>> "Lott, Robert" 11/21/03 12:42PM >>> At the NSH convention in Louisville there was a poster presentation regarding Lab Vision's new RABBIT monoclonal Ab against Estrogen Receptor (Clone SP1)... has anyone else had any experience with this clone OR their new RABBIT monoclonal Ab against CD3 (Clone SP7) or Cyclin D1/bcl-1 (Clone SP4) or Calretinin (Clone SP13)? Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. From bernaweston <@t> hotmail.com Fri Nov 21 13:28:13 2003 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] separated cells continued Message-ID: Hope this helps my problem: Here is our processing program: Formalin 1:00 Formalin 2:30 70% ETOH :50 80% ETOH :50 95% ETOH :50 95% ETOH :50 100% ETOH :50 100% ETOH :50 Paraffin 1:00 Paraffin 1:00 Paraffin 1:00 The paraffin station are at 58 degrees the other stations are ambient the pressure is on all stations. The sections do not appear to be improperly processed when cutting, we use accuride blades, we get a nice ribbon and the sections lay out in the waterbath, we cut at 3 and 4 microns. We put the slides in a 60 degree oven. pictures @ histonet images Bernadette Weston HT _________________________________________________________________ Set yourself up for fun at home! Get tips on home entertainment equipment, video game reviews, and more here. http://special.msn.com/home/homeent.armx From tpmorken <@t> labvision.com Fri Nov 21 14:45:40 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Senior Technologist, Immunohistochemistry position opening, east San Francisco bay area Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD63@usca0082k03.rallansci.apogent.com> Lab Vision Corporation has an opening for a Senior Technologist in the Immunohistochemistry QC and Development laboratory. The successful candidate will have extensive experience in the development of novel antibodies for histology applications with emphasis on optimal labeling methodology. Extensive experience in clinical application of antibody probes to tissue pathology is desired. Additionally the position requires design input and testing of new instruments and other products. The successful candidate will be one who thrives on applying experimental procedures on a daily basis and relishes solving problems in immunohistochemistry. Relocation assistance is possible for the right candidate. Lab Vision is a leader in automation of immunohistochemistry and an innovator in antibody development, most recently with the introduction of Rabbit Monoclonal antibodies. We are also a leader in the research antibody field. We will soon introduce an extensive line of IVD antibodies for the clinical market. Lab Vision is located in Fremont, California, in the East San Franscisco Bay Area, also known as Biotech Bay. See our website at www.labvision.com Please respond to: Tim Morken Product Development Lab Vision / NeoMarkers 47790 Westinghouse Dr Fremont, CA 94539 PH: 510-991-2840 www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/14d57129/attachment.htm From scoop <@t> mail.nih.gov Fri Nov 21 15:50:28 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] phospho alpha synuclein antibodies Message-ID: Does anybody know of a commercial source for antibodies for phosphorylation specific epitopes for alpha synuclein? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From ploykasek <@t> phenopath.com Fri Nov 21 16:46:19 2003 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:15 2005 Subject: FW: [Histonet] Lab Vision's new Rabbit Monoclonal Abs In-Reply-To: Message-ID: ------ Forwarded Message From: Patti Loykasek Date: Fri, 21 Nov 2003 13:05:29 -0800 To: "Lott, Robert" Subject: Re: [Histonet] Lab Vision's new Rabbit Monoclonal Abs > We are currently using the ER-SP1, PR-SP2, CyclinD1-SP4, & testing the > Synaptophysin-SP11. > We did a side by side comparison of these clones of ER/PR and ER-1D5 & PR-636. > The rabbit monoclonals were more sensitive on our series of >200 specimens. > It was a small increase in sensitivity. Of course, I don?t know how these > would compare on your specimens. We should have an abstract at USCAP2004 on > these clones, and probably a paper next year. > The cyclinD1-SP4 is spectacular. Beautiful staining with increased sensitivity > over several clones that we have tried. > We are currently testing the Synaptophysin-SP11. So far, it appears to be > better than our current clone. We have had some weird artifacts with our > current clone that we are not seeing with the SP11. These are very preliminary > results. > If there is any other info that you need, please contact me. > > Patti Loykasek > PhenoPath Laboratories > Seattle, WA > > > At the NSH convention in Louisville there was a poster presentation regarding > Lab Vision?s new RABBIT monoclonal Ab against Estrogen Receptor (Clone SP1)? > has anyone else had any experience with this clone OR their new RABBIT > monoclonal Ab against CD3 (Clone SP7) or Cyclin D1/bcl-1 (Clone SP4) or > Calretinin (Clone SP13)? > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Baptist Health System > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 phone > > 205-592-5646 fax > > robert.lott@bhsala.com > > > > Confidentiality Notice: > The information contained in this email message is privileged and confidential > information and intended only for the use of the individual or entity named in > the address. If you are not the intended recipient, you are hereby notified > that any dissemination, distribution, or copying of this information is > strictly prohibited. If you received this information in error, please notify > the sender and delete this information from your computer and retain no copies > of any of this information. ------ End of Forwarded Message -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/a452710e/attachment.htm From tissuearray <@t> hotmail.com Fri Nov 21 08:33:09 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Dermal Needles for Arrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/4d1c2614/attachment.htm From amosbrooks <@t> earthlink.net Fri Nov 21 20:41:16 2003 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Mushy Peas In-Reply-To: <20031121180001.10951.27794.Mailman@swlx167.swmed.edu> References: <20031121180001.10951.27794.Mailman@swlx167.swmed.edu> Message-ID: <6.0.0.22.0.20031121213555.029ab330@127.0.0.1> EEEch, It's good to know y'all are taking good care of our president. I'll consider myself duely warned if I ever find myself in an english pub. It could have been worse he could have been eating Japaneese fish like his dad :-) Amos Brooks At 01:00 PM 11/21/03, you wrote: >-----Original Message----- >From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] >Sent: 21 November 2003 17:21 >To: Marshall Terry Dr, Consultant Histopathologist >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Political histology>mushy peas > > >Are the peas smashed up or what? I can't quite picture them - like baby = >food??? EEEEwwwww.=20 > >Jacqueline M. O'Connor HT(ASCP) >Abbott Laboratories >Global Pharmaceutical Research and Development >Discovery Chemotheraputics >847.938.4919 >Fax 847.938.3266 From GREYTRUNK <@t> aol.com Fri Nov 21 21:06:44 2003 From: GREYTRUNK <@t> aol.com (GREYTRUNK@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become His totech Message-ID: <116.2bb0da2d.2cf02cc4@aol.com> That is not to say that you couldn't take the HT exam! Roxanne -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/f7cfa36d/attachment.htm From GREYTRUNK <@t> aol.com Fri Nov 21 21:08:37 2003 From: GREYTRUNK <@t> aol.com (GREYTRUNK@aol.com) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become His totech Message-ID: <36.4bce5fec.2cf02d35@aol.com> However--if one fails the exam 5 times, one would have to ask themselves if this is what one should be doing. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031121/9dad0ab7/attachment.htm From ekaplan <@t> squ.edu.om Fri Nov 21 22:43:17 2003 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Mushy Peas In-Reply-To: <6.0.0.22.0.20031121213555.029ab330@127.0.0.1> Message-ID: Good morning, Mushy peas are really no different in consistency to re-fried beans! Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Amos Brooks Sent: Saturday, November 22, 2003 6:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushy Peas EEEch, It's good to know y'all are taking good care of our president. I'll consider myself duely warned if I ever find myself in an english pub. It could have been worse he could have been eating Japaneese fish like his dad :-) Amos Brooks At 01:00 PM 11/21/03, you wrote: >-----Original Message----- >From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] >Sent: 21 November 2003 17:21 >To: Marshall Terry Dr, Consultant Histopathologist >Cc: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Political histology>mushy peas > > >Are the peas smashed up or what? I can't quite picture them - like baby = >food??? EEEEwwwww.=20 > >Jacqueline M. O'Connor HT(ASCP) >Abbott Laboratories >Global Pharmaceutical Research and Development >Discovery Chemotheraputics >847.938.4919 >Fax 847.938.3266 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mrl0627 <@t> mail.ecu.edu Sat Nov 22 11:55:31 2003 From: mrl0627 <@t> mail.ecu.edu (mrl0627@mail.ecu.edu) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Anti-beta-galactosidase antibodies Message-ID: The easy answer is,I suppose, "because my thesis committee wants it that way" (smile). Working with hemizygote (lethal mutation in homozygotes) of transgenic mouse with LacZ insertion in front of proteoglycan of interest, enzyme histo equiv (maybe endogenous bgal plus weak signal in tissue) and have been doing immuno for core protein on Xgal reacted slides. Working fine. DAB looks beautiful next to Xgal blue. Complication is that the other grad students in our lab use flourescent antibodies and apparently are getting some kind of autoflourescent problem (don't know the details)when they use 40-1a on previously xgal reacted tissue. My advisor (who REALLY wants immuno and xgal on same sections) wants me to see how it 40-1a looks on my slides and if I still have the false positive problem. So trying to slay more than 1 dragon! Thanks for the reply. Maureen -----Original Message----- From: Sharon Cooperman <scoop@mail.nih.gov> To: mrl0627@mail.ecu.edu Sent: Wed, 19 Nov 2003 09:40:27 -0500 Subject: Re: [Histonet] Anti-beta-galactosidase antibodies No, sorry. Just out of curiousity, why do you want to do both the enzymatic and IHC? Also, I think that if the enzyme isn't destroyed by the reaction it might work - eg., I wouldn't expect it to work on peroxidase, but I don't know if beta gal activity persists after the reaction. Sharon >Sharon, >Have you used this antibody on previously X-gal reacted tissue? We >are using 40-1a. Thanks, Maureen Loomer, MS candidate at ECU > >-----Original Message----- >From: Sharon Cooperman <scoop@mail.nih.gov> >To: t-kuzniar@md.northwestern.edu >Sent: Tue, 18 Nov 2003 18:34:04 -0500 >Subject: Re: [Histonet] Anti-beta-galactosidase antibodies > >Hi. Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed >paraffin embedded tissue. We used it on a transgenic mouse >expressing Beta gal - pretty weak but detectable with ABC kit and >HRP. I think most of the transgenics express weakly and I would >consider using tyramide (TSA) if you need more signal because >background is very low. I also think some of the Abcam anti-beta gal >abs are pretty good (I don't know which ones, I borrowed them). > >Sharon > >>Has anyone had any experience with polyclonal antibody against >>b-galactosidase? What reporter system have you used? Thank you. >>Tom Kuzniar >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >-- >Sharon Cooperman <scoop@mail.nih.gov> >NIH, NICHD, CBMB 301.435-7735 >Building 18T, room 101 301.402-0078 fax >Bethesda, MD 20892 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Sharon Cooperman <scoop@mail.nih.gov> NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From hodges420 <@t> msn.com Sat Nov 22 17:31:24 2003 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] JCHO/CAP Message-ID: Good evening all, A while ago, I thought I read on the Histonet that nothing but soap or wash type products could be kept under sinks. Can some one refresh my memory. I am at a new Zhospital here in town (Tucson) and they have JCHO in 1 week. Thanks Tere Hodges St Mary's Hospital Tucson, Az. HT,ASCP _________________________________________________________________ online games and music with a high-speed Internet connection! Prices start at less than $1 a day average. https://broadband.msn.com (Prices may vary by service area.) From dave_laidley <@t> yahoo.ca Sun Nov 23 09:40:31 2003 From: dave_laidley <@t> yahoo.ca (David Laidley) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] DAB and fading Message-ID: <20031123154031.31865.qmail@web20802.mail.yahoo.com> I am currently staining brain tissue (perfused 4% paraformaldehyde) that has been cut in a cryostat at 50 microns and I am staining for NeuN using DAB tablets with Cobalt from Sigma. The stain works perfectly. I am using a mounting medium that is glycerol based (PVA-glycerol). This mounting medium also works perfectly as it can be quickly applied to the sections after they have been mounted on a slide which perserves the thickness of the sections (I am interested in doing stereology) and it polymerizes overnight so that nail polish is not necessary to keep the coverlip on. (I have used the same medium with fluorescent tissue except DABCO was added) But I am noticing that over a number of days that may sections which have neurons stained black, turn brown and pale. Is this fading a product of the mounting medium? Does the fading progress to where the precipitate can no longer be seen? Has anyone used aqueous mounting mediums with DAB and had fading? It was my understanding that DAB didn't fade (or at least not too much). Comments? Suggestions? Please help me put my concerns to rest. David Laidley MSc student Memorial University of Newfoundland Division of Basic Medical Sciences - Neurosciences Group --------------------------------- Post your free ad now! Yahoo! Canada Personals -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031123/263f251a/attachment.htm From jqb7 <@t> cdc.gov Sun Nov 23 10:01:09 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: And we all know what lousy histotechs pathologists make! -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu To: Histonet (E-mail) Sent: 11/21/2003 11:34 AM Subject: RE: 5-time limit. [Histonet] Deadline for 2 yr OJT to become Histotech Bruce wrote: << What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again?> Brue, sorry to say, that's right, You can't be a certified histotech. Your dreams are shattered and you will be forced to become a pathologist if you want to keep working in the field. Tim Morken -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Friday, November 21, 2003 5:39 AM To: 'Lee & Peggy Wenk'; Histonet (E-mail) Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again? Just a thought. Bruce Chung Anatomic Pathology Manager -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Friday, November 21, 2003 6:05 AM To: Peggy Micciche; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech One (long) added comment to Peggy's and Frieda's comments. A candidate must TAKE the HT exam before Jan. 2005. However, they do not have to PASS the exam before that deadline. When someone signs up to take the HT exam (or other ASCP certification exams), they are given a 5 year time frame. Also, they have 5 times in which to take the exam. EXAMPLE: If the person takes the HT exam for the first time in April 2004 (second cycle), and does not pass it, and then tries again in Oct. 2004 (4th cycle), and still does not pass it, they still have 3 more times to take and pass the exam. Since they first took the exam in the 2nd cycle of 2004, they would have 2005, 2006, 2007, 2009 (just Jan -Mar 2009 = first cycle) in which to pass in one of their last 3 tries. Now remember, they have to pass both the written and practical exam. But not necessarily at the same time. EXAMPLE: If the person passes the practical on the first try, but not the written, they just have to repeat the written. Their passing practical grade is good for that 5 year period. Whenever they pass the written (say, the 3rd time), the two passing scores are combined, and they have now passed the HT certification exam. If someone who qualifies through Route 3 (high school diploma + 2 years OJT), but does not take the exam for the first time before Jan. 2005, they can still go on and earn their associate degree with 20 hours biology/chemistry, and then take the HT exam in the future via the associate degree route (Route 2). Or, if they can get into a NAACLS-accredited HT program, they can still take the HT exam in the future via that route (Route 1). However, if someone trying via the high school diploma route uses up all of their 5 chances over the next 5 years, and then in the future earns their associate degree with the required bio/chem, they would NOT be allowed to retake the HT certification exam. A candidate is given a total of 5 times to take a specific certification exam, regardless of how many different routes through which they try. If, on the other hand, they could continue with their education, earn their baccalaureate degree with 30 hours of bio/chem, they can now take the HTL exam, and be given 5 new chances. They are now taking a DIFFERENT certification exam. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Peggy Micciche" To: Sent: Thursday, November 20, 2003 11:44 AM Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech > You must apply by July 1, 2004 to be eligible to take the exam via Route 3 > which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at > www.ascp.org for more information. > > Peggy Micciche > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > FreidaC@aol.com > Sent: Thursday, November 20, 2003 10:10 AM > To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech > > > I do not believe it is correct that you have until December 31, 2004 to sign > up for the ASCP certifying exam. Without an associate degree, you must TAKE > exam for the first time before December 31, 2004. This obviously means that > you must register for it much earlier. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031123/76dd4014/attachment.htm From AnthonyH <@t> chw.edu.au Sun Nov 23 16:22:57 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] separated cells continued Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0BD@simba.kids> Where is the xylene or other wax & ethanol miscible solvent? Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Bernadette Weston [mailto:bernaweston@hotmail.com] Sent: Saturday, 22 November 2003 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] separated cells continued Hope this helps my problem: Here is our processing program: Formalin 1:00 Formalin 2:30 70% ETOH :50 80% ETOH :50 95% ETOH :50 95% ETOH :50 100% ETOH :50 100% ETOH :50 Paraffin 1:00 Paraffin 1:00 Paraffin 1:00 The paraffin station are at 58 degrees the other stations are ambient the pressure is on all stations. The sections do not appear to be improperly processed when cutting, we use accuride blades, we get a nice ribbon and the sections lay out in the waterbath, we cut at 3 and 4 microns. We put the slides in a 60 degree oven. pictures @ histonet images Bernadette Weston HT _________________________________________________________________ Set yourself up for fun at home! Get tips on home entertainment equipment, video game reviews, and more here. http://special.msn.com/home/homeent.armx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From ctsblack <@t> capeheart.uct.ac.za Mon Nov 24 04:21:56 2003 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] RE: Saw/Grind Sections Message-ID: Hello fellow Resin Workers Can you shed some light onto a small problem?? I am finding that when I grind my saw-cut sections using 2 grades of sand paper, (600/1200) fine black spots are settling on the tissue. I have tried to wash the out with water, but they don't come off?? It must be tiny pieces of dust from the sand paper. I am using water all the time, and clean paper. Any ideas?? Thank you Melanie Black -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From Jackie.O'Connor <@t> abbott.com Mon Nov 24 07:10:36 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become His totech Message-ID: JFK Jr. failed to pass the bar exam the first 3 times he took it. When he passed the 4th time, he got a job for $35,000 per year. That was in 1989 (I may be off one year). GREYTRUNK@aol.com Sent by: histonet-admin@lists.utsouthwestern.edu 11/21/2003 09:08 PM To: GREYTRUNK@aol.com, tpmorken@labvision.com, histonet@lists.utsouthwestern.edu cc: Subject: Re: 5-time limit. [Histonet] Deadline for 2 yr OJT to become His totech However--if one fails the exam 5 times, one would have to ask themselves if this is what one should be doing. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031124/d1958d93/attachment.htm From tissuearray <@t> hotmail.com Mon Nov 24 07:27:09 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Re: Manual embedding of tissue cores for arrays Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031124/03a4d1fe/attachment.htm From froyer <@t> bitstream.net Mon Nov 24 07:58:44 2003 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:15 2005 Subject: 5-time limit. [Histonet] Deadline for 2 yr OJT to become His totech In-Reply-To: References: Message-ID: <3FC20E94.6010502@bitstream.net> One of the memories of my 10 years in the lab was the strange phenomenon that the best techs that I ever had were the ones that had difficulty passing the Registry on the first try. The best tech (M.T.) that I ever worked with in my life took it 4 times before success. We can all remember techs that seemed to be one slice short of a loaf when it came to daily routine workloads and were a ways behind the curve in productivity. It seemed that these individuals, in my personal experience, more times than not, were the ones who passed the Registry on the first try. One of Life's little mysteries. ;-) ~ Ford Ford M. Royer, MT(ASCP) Analytical Instruments, LLC Minneapolis, MN 800-565-1895, Ext. 17 Jackie.O'Connor@abbott.com wrote: > > JFK Jr. failed to pass the bar exam the first 3 times he took it. > When he passed the 4th time, he got a job for $35,000 per year. That > was in 1989 (I may be off one year). > > > > > > GREYTRUNK@aol.com > Sent by: histonet-admin@lists.utsouthwestern.edu > > 11/21/2003 09:08 PM > > > To: GREYTRUNK@aol.com, tpmorken@labvision.com, > histonet@lists.utsouthwestern.edu > cc: > Subject: Re: 5-time limit. [Histonet] Deadline for 2 yr > OJT to become His totech > > > > > However--if one fails the exam 5 times, one would have to ask > themselves if this is what one should be doing. > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031124/c83443aa/attachment.htm From mprice26 <@t> juno.com Mon Nov 24 08:39:07 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Continuous Microwave Processor in CAP Today Message-ID: <20031124.063946.20461.233832@webmail13.nyc.untd.com> Histonetters, Does anyone know who to contact for a demo and pricing on the Contiuous Microwave Processor that was featured in October 2003 Vol. 17 No.10 CAP TODAY? Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From jqb7 <@t> cdc.gov Mon Nov 24 08:46:58 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Continuous Microwave Processor in CAP Today Message-ID: Call Sharon Wehman with Sakura. (800) 725-8723. It is an amazing machine! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of mprice26@juno.com Sent: Monday, November 24, 2003 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Continuous Microwave Processor in CAP Today Histonetters, Does anyone know who to contact for a demo and pricing on the Contiuous Microwave Processor that was featured in October 2003 Vol. 17 No.10 CAP TODAY? Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thallada <@t> noch.org Mon Nov 24 08:58:09 2003 From: thallada <@t> noch.org (Hallada, Teri) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Continuous Microwave Processor in CAP Today Message-ID: It's wonderful, but be prepared for the price tag. I was told at the ASC meeting two weeks ago that it will be around $200,000. Ouch! I was also told that they have no plans for a smaller version. Teri Hallada BS MT CT (ASCP) thallada@noch.org > -----Original Message----- > From: mprice26@juno.com [SMTP:mprice26@juno.com] > Sent: Monday, November 24, 2003 09:39 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Continuous Microwave Processor in CAP Today > > > Histonetters, > Does anyone know who to contact for a demo and pricing on the Contiuous Microwave Processor that was featured in October 2003 Vol. 17 No.10 CAP TODAY? > > Thank you. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Nov 24 09:02:20 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] DAB and fading In-Reply-To: <20031123154031.31865.qmail@web20802.mail.yahoo.com> References: <20031123154031.31865.qmail@web20802.mail.yahoo.com> Message-ID: <3FC21D7C.2080600@umdnj.edu> Sounds like the DAB is being oxidized by something in the mounting medium. John Kiernan has recipes for non-fading mounting media on his website that you can make yourself. Ringing the coverslips with nail polish may not be "necessary" but if air is getting to the sections that may be making the problem worse. Do the experiment yourself, ring some with nail polish and see if it helps. DAB (even with intensification) does fade (after a few years) in Permount too, that is why I use only DPX these days. Geoff David Laidley wrote: > I am currently staining brain tissue (perfused 4% paraformaldehyde) > that has been cut in a cryostat at 50 microns and I am staining for > NeuN using DAB tablets with Cobalt from Sigma. > > The stain works perfectly. I am using a mounting medium that is > glycerol based (PVA-glycerol). This mounting medium also works > perfectly as it can be quickly applied to the sections after they have > been mounted on a slide which perserves the thickness of the sections > (I am interested in doing stereology) and it polymerizes overnight so > that nail polish is not necessary to keep the coverlip on. (I have > used the same medium with fluorescent tissue except DABCO was added) > > But I am noticing that over a number of days that may sections which > have neurons stained black, turn brown and pale. Is this fading a > product of the mounting medium? Does the fading progress to where the > precipitate can no longer be seen? Has anyone used aqueous mounting > mediums with DAB and had fading? It was my understanding that DAB > didn't fade (or at least not too much). > > Comments? Suggestions? Please help me put my concerns to rest. > > David Laidley > > MSc student > > Memorial University of Newfoundland > > Division of Basic Medical Sciences - Neurosciences Group > > > > > > > ------------------------------------------------------------------------ > Post your free ad now! Yahoo! Canada Personals > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Barry.R.Rittman <@t> uth.tmc.edu Mon Nov 24 09:28:19 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] RE: Saw/Grind Sections Message-ID: <566FB0B522443D43AF02D2ADBE35A6F06358B6@UTHEVS3.mail.uthouston.edu> Melanie This can occur from time to time but I found it to be more common using sections from plastic embedded tissue. The problem with plastic sections especially with embedded bone seems to be that the abrasive is significantly harder than the plastic and the tissue and therefore gets embedded in the surface. One solution that I found useful was to go to a frosted glass plate and with just distilled water use this to polish the surfaces. This will often remove some if not all the particles. The frosted surface can be prepared using alumina and washing well. Off the topic My daughter, who is a research chef, just visited Cape Town on business and was very impressed with the beautiful scenery, the food, and the friendliness of everyone there. Thank you. Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Melanie Black Sent: Monday, November 24, 2003 4:22 AM To: histonet@pathology.swmed.edu Subject: [Histonet] RE: Saw/Grind Sections Hello fellow Resin Workers Can you shed some light onto a small problem?? I am finding that when I grind my saw-cut sections using 2 grades of sand paper, (600/1200) fine black spots are settling on the tissue. I have tried to wash the out with water, but they don't come off?? It must be tiny pieces of dust from the sand paper. I am using water all the time, and clean paper. Any ideas?? Thank you Melanie Black -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rdr2002 <@t> med.cornell.edu Mon Nov 24 11:00:46 2003 From: rdr2002 <@t> med.cornell.edu (Rebecca D. Riba) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] GFP mounting media Message-ID: <1286.140.251.150.219.1069693246.squirrel@webmail> Hello, I have been reading the list for recommendations on mounting media used for tissues expressing GFP. I have seen arguments on PBS versus antifade medias (vectashield, prolong, homemade, etc.). In addition, discussion of the use of fresh frozen, OCT frozen, and paraffin. Does anyone have any additional recommendations on these protocols for visualizing egfp or dsred expressing tissues. In addition, has anybody used vectashields hard-set mounting media? Thank you, Rebecca Riba Weill Medical College of Cornell From Janet.Bonner <@t> FLHOSP.ORG Mon Nov 24 12:31:45 2003 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] JCHO/CAP Message-ID: <9F4410664F56DC4A8B2BD42920756D0C490C9D@fh2k093.fhmis.net> Our hospital screwed the "under-the-sink" area doors to the frame as we were made to understand that NOTHING could be stored in that area (because of contamination - even soap that we wash hands/tools/glassware with)! :) -----Original Message----- From: MARY T HODGES [mailto:hodges420@msn.com] Sent: Saturday, November 22, 2003 6:31 PM To: histonet@pathology.swmed.edu Subject: [Histonet] JCHO/CAP Good evening all, A while ago, I thought I read on the Histonet that nothing but soap or wash type products could be kept under sinks. Can some one refresh my memory. I am at a new Zhospital here in town (Tucson) and they have JCHO in 1 week. Thanks Tere Hodges St Mary's Hospital Tucson, Az. HT,ASCP _________________________________________________________________ online games and music with a high-speed Internet connection! Prices start at less than $1 a day average. https://broadband.msn.com (Prices may vary by service area.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From Diane.Gladney <@t> se.amedd.army.mil Mon Nov 24 12:40:23 2003 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] JCHO/CAP Message-ID: <9D41AB7C56F8304F98537ABD87B249F662616A@dasmthgbz001.amedd.army.mil> Mary, We had our JCHO inspection this year. We had to remove everything from under the sink except liquid cleaner and bleach. We cannot store powdered soap like "Alconox", etc. We even had to take the brushes out that we use to scrub the glassware with. The inspector looked under our sink and gave a "OKAY ! This looks great." So I would think that you can store your liquid cleaners under there but nothing else. Hope this helps. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: MARY T HODGES [mailto:hodges420@msn.com] Sent: Saturday, November 22, 2003 6:31 PM To: histonet@pathology.swmed.edu Subject: [Histonet] JCHO/CAP Good evening all, A while ago, I thought I read on the Histonet that nothing but soap or wash type products could be kept under sinks. Can some one refresh my memory. I am at a new Zhospital here in town (Tucson) and they have JCHO in 1 week. Thanks Tere Hodges St Mary's Hospital Tucson, Az. HT,ASCP _________________________________________________________________ online games and music with a high-speed Internet connection! Prices start at less than $1 a day average. https://broadband.msn.com (Prices may vary by service area.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BRIAN.CHELACK <@t> usask.ca Mon Nov 24 13:24:19 2003 From: BRIAN.CHELACK <@t> usask.ca (Brian Chelack) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] Re: DAB fading Message-ID: <3FC25AE3.85D23995@sask.usask.ca> Our experience with DAB is that it will fade slowly on exposure to light. Are your slides in close proximity to any flourescent light sources? The amount of fading can also be related to the source of DAB. We have found the DAB from Electron Microscopy Sciences to be the best for our purposes. Brian Chelack Prairie Diagnostic Services From CTague <@t> ahs.llumc.edu Mon Nov 24 14:56:39 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] if doing immunos Message-ID: how many should be done per day by a small lab to "break even" or make money? also, what is the best low cost stainer out there? i expect to hear they go for over 50k... which is why we don't have one. LOL Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From CMCCOLLOUGH <@t> dnr.state.md.us Mon Nov 24 15:10:09 2003 From: CMCCOLLOUGH <@t> dnr.state.md.us (McCollough, Carol) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] microtome maintenance Message-ID: <4D36F3AA1525DF408493B4E1FDE9EDE50B38FA@centrevilleex.langroup.dnr.md> Greetings Histonetters: Any recommendations of companies or individuals who perform microtome maintenance and repair in the mid-Atlantic region? I have 3 Microms (325 & 330) and an old AO. Thanks for your help. Regards - Carol ****************** Carol B. McCollough, HT/HTL (ASCP) Diagnostics & Histology Laboratory Manager Maryland Department of Natural Resources Cooperative Oxford Laboratory 904 S. Morris Street Oxford, Maryland 21654 cmccollough@dnr.state.md.us From cgfields <@t> lexhealth.org Mon Nov 24 15:30:42 2003 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] microtome maintenance Message-ID: Southeast Pathology Instrument Service (Michael Dietrich) Tel. (843)588-9456 email Yarbo3@aol.com does all our repairs. We have 5 automatic Microm microtomes, 2 Microm cryostats, 3 VIP's etc. He can repair most anything. He does an excellent job and is very nice. Carole Fields Lex Med Ctn W.Columbia, SC > -----Original Message----- > From: McCollough, Carol [SMTP:CMCCOLLOUGH@dnr.state.md.us] > Sent: Monday, November 24, 2003 4:10 PM > To: Histonet (E-mail 2) > Subject: [Histonet] microtome maintenance > > Greetings Histonetters: > > Any recommendations of companies or individuals who perform microtome > maintenance and repair in the mid-Atlantic region? I have 3 Microms (325 > & 330) and an old AO. > > Thanks for your help. > > Regards - > Carol > ****************** > Carol B. McCollough, HT/HTL (ASCP) > Diagnostics & Histology Laboratory Manager > Maryland Department of Natural Resources > Cooperative Oxford Laboratory > 904 S. Morris Street > Oxford, Maryland 21654 > cmccollough@dnr.state.md.us > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031124/b3b1743d/attachment.htm From pruegg <@t> colobio.com Mon Nov 24 15:57:33 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:15 2005 Subject: [Histonet] if doing immunos In-Reply-To: Message-ID: Curtis, Some companies offer the use of their autostainers with a reagent requisition contract which may not be as much as you may think. I have a DAKO autostainer this way. The DAKO is an open system which means you are not locked in to their reagents. Patsy Ruegg -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tague, Curtis Sent: Monday, November 24, 2003 1:57 PM To: Histonet (E-mail) Subject: [Histonet] if doing immunos how many should be done per day by a small lab to "break even" or make money? also, what is the best low cost stainer out there? i expect to hear they go for over 50k... which is why we don't have one. LOL Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJones <@t> cvm.tamu.edu Mon Nov 24 17:20:21 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Deadline for 2 yr OJT to become Histotech Message-ID: OMG, if they fail 5 times, maybe they should look for another profession!!!! Or we could just give them the ASCP tombstone shaped sticker right off the bat, they won't have to wait 25 years like the rest of us. Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Chung, Luong" 11/21/2003 7:39:09 AM >>> What happen if they fail 5 times with the HTL route plus a BS degree? They can't become a Histotech ever again? Just a thought. Bruce Chung Anatomic Pathology Manager -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Friday, November 21, 2003 6:05 AM To: Peggy Micciche; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech One (long) added comment to Peggy's and Frieda's comments. A candidate must TAKE the HT exam before Jan. 2005. However, they do not have to PASS the exam before that deadline. When someone signs up to take the HT exam (or other ASCP certification exams), they are given a 5 year time frame. Also, they have 5 times in which to take the exam. EXAMPLE: If the person takes the HT exam for the first time in April 2004 (second cycle), and does not pass it, and then tries again in Oct. 2004 (4th cycle), and still does not pass it, they still have 3 more times to take and pass the exam. Since they first took the exam in the 2nd cycle of 2004, they would have 2005, 2006, 2007, 2009 (just Jan -Mar 2009 = first cycle) in which to pass in one of their last 3 tries. Now remember, they have to pass both the written and practical exam. But not necessarily at the same time. EXAMPLE: If the person passes the practical on the first try, but not the written, they just have to repeat the written. Their passing practical grade is good for that 5 year period. Whenever they pass the written (say, the 3rd time), the two passing scores are combined, and they have now passed the HT certification exam. If someone who qualifies through Route 3 (high school diploma + 2 years OJT), but does not take the exam for the first time before Jan. 2005, they can still go on and earn their associate degree with 20 hours biology/chemistry, and then take the HT exam in the future via the associate degree route (Route 2). Or, if they can get into a NAACLS-accredited HT program, they can still take the HT exam in the future via that route (Route 1). However, if someone trying via the high school diploma route uses up all of their 5 chances over the next 5 years, and then in the future earns their associate degree with the required bio/chem, they would NOT be allowed to retake the HT certification exam. A candidate is given a total of 5 times to take a specific certification exam, regardless of how many different routes through which they try. If, on the other hand, they could continue with their education, earn their baccalaureate degree with 30 hours of bio/chem, they can now take the HTL exam, and be given 5 new chances. They are now taking a DIFFERENT certification exam. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: "Peggy Micciche" To: Sent: Thursday, November 20, 2003 11:44 AM Subject: RE: [Histonet] Deadline for 2 yr OJT to become Histotech > You must apply by July 1, 2004 to be eligible to take the exam via Route 3 > which will be discontinued as of Jan. 2005. Visit the ASCP-BOR site at > www.ascp.org for more information. > > Peggy Micciche > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > FreidaC@aol.com > Sent: Thursday, November 20, 2003 10:10 AM > To: GREYTRUNK@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Deadline for 2 yr OJT to become Histotech > > > I do not believe it is correct that you have until December 31, 2004 to sign > up for the ASCP certifying exam. Without an associate degree, you must TAKE > exam for the first time before December 31, 2004. This obviously means that > you must register for it much earlier. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clzindl <@t> artsci.wustl.edu Mon Nov 24 20:44:40 2003 From: clzindl <@t> artsci.wustl.edu (Carlene L. Zindl) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] In situ hybridization In-Reply-To: <20031124180001.20537.91738.Mailman@swlx167.swmed.edu> References: <20031124180001.20537.91738.Mailman@swlx167.swmed.edu> Message-ID: Hello, Does anyone have experience with non-radioactive in situ hybridization of frozen mouse tissue?? If so, I would appreciate advice on details such as: 1) is biotin or dig better? 2) what is the best way to fix this tissue? 3) what is the optimal size RNA probe? Thank you for any pertinent information. Sincerely, Carlene Zindl University of Alabama at Birmingham clzindl@artsci.wustl.edu -OR- clzindl@uab.edu From caroline.stott <@t> anatomy.otago.ac.nz Mon Nov 24 14:01:12 2003 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Whole bee embryo fixation Message-ID: <5.2.1.1.0.20031125085941.00a41bf0@anatomy.otago.ac.nz> Hello, Has anyone had any experience with whole bee embryo fixation? We can bleach off the outer membrane which is no problem. However we are having trouble with fixatives penetrating the inner membrane. If we make a hole in it, the embryo explodes. Can anyone help? Thanks Caroline Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From louise_renton <@t> hotmail.com Tue Nov 25 01:43:32 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: Could someone please expand on this? Obviously I am an ignorant South African, but by "contamination" do you mean leakage of waste water? And if your controls are as strict as I am led to believe (no oozing blood and guts or toxic chemicals going down the drain) why does this constitute a problem?. I am afraid that I am missing something simple here. Unless, could it be that there are more crawling infants in histolabs in the USA than I know of :>)!!!!!? Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ---- Our hospital screwed the "under-the-sink" area doors to the frame as we were made to understand that NOTHING could be stored in that area (because of contamination - even soap that we wash hands/tools/glassware with)! :) -----Original Message----- From: MARY T HODGES [ Sent: Saturday, November 22, 2003 6:31 PM To: histonet@pathology.swmed.edu Subject: [Histonet] JCHO/CAP Good evening all, A while ago, I thought I read on the Histonet that nothing but soap or wash type products could be kept under sinks. Can some one refresh my memory. I am at a new Zhospital here in town (Tucson) and they have JCHO in 1 week. Thanks Tere Hodges St Mary's Hospital Tucson, Az. HT,ASCP _________________________________________________________________ online games and music with a high-speed Internet connection! Prices start at less than $1 a day average. https://broadband.msn.com (Prices may vary by service area.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your kids can now chat online in a safe, controlled environment! http://messenger.msn.co.za/ From peter.smale <@t> nhls.ac.za Tue Nov 25 02:17:24 2003 From: peter.smale <@t> nhls.ac.za (Peter Smale) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] restaining of hematoxylin and eosin tissue sections Message-ID: <000a01c3b32c$92a73370$af06a8c0@PeterSmale> I wonder if someone can assist me in solving this problem.I retrieved a hematoxylin and eosin/phloxine stained slide from 1998 and discovered it was faded.The tissue section was mounted in a coverslipping machine that uses coverslipping tape composed of cellulose triacetate and is coated on one side with resinous mountant.This is the procedure I followed to decoverslip,decolorise and restain: I placed the slide in acetone for 7 minutes to remove the coverslip and then put the slide in xylene for 6 hours to remove any residual resinous mountant.I took the slide through graded ethanols to water after which I decolorised with acid/alcohol for one minute, took the slide back to water and restained the nuclei with Gill's Hematoxylin ( for 5 min. ) and counterstained with a mixture of 2% eosin y and 1% phloxine B with 4 drops of conc. glacial acetic acid added ( for 3 min. ).The nuclear staining turned out pale and the counter stain was patchy almost as if something was blocking the tissue's ability to retain the dyes I cannot figure out why the tissue is not staining with the same intensity as it did in 1998.Please keep in mind the slide was left out in natural light for a while which resulted in it becoming faded. ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg, South Africa tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031125/dcd02abd/attachment.htm From peter.smale <@t> nhls.ac.za Tue Nov 25 05:15:14 2003 From: peter.smale <@t> nhls.ac.za (Peter Smale) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] restaining of hematoxylin and eosin tissue sections References: <000201c3b330$3a005bd0$024dbad0@hppav> Message-ID: <001001c3b345$cd7f1940$af06a8c0@PeterSmale> George Cole The slide was left out exposed to sunlight for a prolonged period, probably several weeks and yes all our slides are coverslipped using the same coverslipping tape. ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- ----- Original Message ----- From: George Cole To: 'Peter Smale' Sent: Tuesday, November 25, 2003 10:43 AM Subject: RE: [Histonet] restaining of hematoxylin and eosin tissue sections Peter, No clear answers from me, but one vague guess---- What caused the H & E to fade? H & E's seem to hold up pretty well. Did a lot of people look at the slide for prolonged periods under bright light? If not, I would consider the covering method----that's the only difference that I can see between that H & E and a million other unfaded H & E's. It doesn't seem likely that it involved the reagents you used to remove the cover and the residual of the covering, because the fading had already taken place. Do you have any other H & E's covered the same way? How are they? I hope you solve your problem. georgecole@ev1.net . -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Peter Smale Sent: Tuesday, November 25, 2003 12:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] restaining of hematoxylin and eosin tissue sections Importance: High I wonder if someone can assist me in solving this problem.I retrieved a hematoxylin and eosin/phloxine stained slide from 1998 and discovered it was faded.The tissue section was mounted in a coverslipping machine that uses coverslipping tape composed of cellulose triacetate and is coated on one side with resinous mountant.This is the procedure I followed to decoverslip,decolorise and restain: I placed the slide in acetone for 7 minutes to remove the coverslip and then put the slide in xylene for 6 hours to remove any residual resinous mountant.I took the slide through graded ethanols to water after which I decolorised with acid/alcohol for one minute, took the slide back to water and restained the nuclei with Gill's Hematoxylin ( for 5 min. ) and counterstained with a mixture of 2% eosin y and 1% phloxine B with 4 drops of conc. glacial acetic acid added ( for 3 min. ).The nuclear staining turned out pale and the counter stain was patchy almost as if something was blocking the tissue's ability to retain the dyes I cannot figure out why the tissue is not staining with the same intensity as it did in 1998.Please keep in mind the slide was left out in natural light for a while which resulted in it becoming faded. ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg, South Africa tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031125/fa13304e/attachment.htm From louise_renton <@t> hotmail.com Tue Nov 25 06:57:33 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] restaining of hematoxylin and eosin tissue sections Message-ID: Did you hand stain or machine stain the slide? If it was automated, perhaps the surface tension on the slide was altered in some way and the reagents did not have the opportunity to "stick" onto the section., perhaps the graded alcohols are contaminated with xylene. Some suggestions: a) Rinse slide in fresh abs alc b) Try restaining by hand to monitor progress c) Use the counterstain with the acetic acid omitted. This might be fading your nuclear staining. ( I seem to recall a pernicious habit of "dosing" the eosin with acetic acid as the week wore on - after a couple of days it was sufficient to wash out the haem) Best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "Peter Smale" Reply-To: "Peter Smale" To: Subject: [Histonet] restaining of hematoxylin and eosin tissue sections Date: Tue, 25 Nov 2003 10:17:24 +0200 I wonder if someone can assist me in solving this problem.I retrieved a hematoxylin and eosin/phloxine stained slide from 1998 and discovered it was faded.The tissue section was mounted in a coverslipping machine that uses coverslipping tape composed of cellulose triacetate and is coated on one side with resinous mountant.This is the procedure I followed to decoverslip,decolorise and restain: I placed the slide in acetone for 7 minutes to remove the coverslip and then put the slide in xylene for 6 hours to remove any residual resinous mountant.I took the slide through graded ethanols to water after which I decolorised with acid/alcohol for one minute, took the slide back to water and restained the nuclei with Gill's Hematoxylin ( for 5 min. ) and counterstained with a mixture of 2% eosin y and 1% phloxine B with 4 drops of conc. glacial acetic acid added ( for 3 min. ).The nuclear staining turned out pale and the counter stain was patchy almost as if something was blocking the tissue's ability to retain the dyes I cannot figure out why the tissue is not staining with the same intensity as it did in 1998.Please keep in mind the slide was left out in natural light for a while which resulted in it becoming faded. ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg, South Africa tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- _________________________________________________________________ Your kids can now chat online in a safe, controlled environment! http://messenger.msn.co.za/ From histo <@t> bthosp.com Tue Nov 25 07:47:32 2003 From: histo <@t> bthosp.com (O'Brien, Sue) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline?) Message-ID: <3653750027C5D6119D0600508BB89A9A0ABF86@MAIL_SERVER> I was just wondering how other Histology Labs receive their specimens from the Operating Room (OR). Also, how do the specimens come to the Lab? (Do Histology personnel pick up the specimens? Or do OR personnel or a separate transport service bring them?) If you could include how many beds your facility has as well, I'd appreciate it. Thanks, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 e-mail: histo@bthosp.com From bmalgrange <@t> ulg.ac.be Tue Nov 25 08:20:08 2003 From: bmalgrange <@t> ulg.ac.be (Brigitte Malgrange) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] beta-gal staining Message-ID: Dear all, I am looking for a beta-galactosidase substrate which can work on PFA 4% fixed sections. A substrat like X-gal, but which work in fluorescence (FITC or TRITC wavelengths). I have found fluoro-deoxy-glucose, but I haven't seen any publication which mention its use on fixed sections. Thanks a lot Brigitte malgrange ---------------------------------------------------------- Dr Brigitte Malgrange University of Li?ge Center for cellular and molecular neuroscience 17 place delcour B-4020 LIEGE Tel : +32 43 66 59 18 Fax : +32 43 66 59 12 e-mail : bmalgrange@ulg.ac.be From GauchV <@t> mail.amc.edu Tue Nov 25 08:49:19 2003 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline?) Message-ID: We receive our OR specimens fresh and they are brought to the Frozen Section Room located in the OR by OR personnel. Our hospital has apprximately 630 beds. Vicki Gauch AMCH Albany, NY >>> "O'Brien, Sue" 11/25/03 8:47:32 AM >>> I was just wondering how other Histology Labs receive their specimens from the Operating Room (OR). Also, how do the specimens come to the Lab? (Do Histology personnel pick up the specimens? Or do OR personnel or a separate transport service bring them?) If you could include how many beds your facility has as well, I'd appreciate it. Thanks, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 e-mail: histo@bthosp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Nov 25 08:56:05 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Whole bee embryo fixation In-Reply-To: <5.2.1.1.0.20031125085941.00a41bf0@anatomy.otago.ac.nz> References: <5.2.1.1.0.20031125085941.00a41bf0@anatomy.otago.ac.nz> Message-ID: <3FC36D85.7050309@umdnj.edu> Check out "Phase partition fixation and staining of Drosophila eggs" by Zalokar and Erb, Stain Technology 52:89, 1977. Caroline Stott wrote: > Hello, > Has anyone had any experience with whole bee embryo fixation? We can > bleach off the outer membrane which is no problem. However we are > having trouble with fixatives penetrating the inner membrane. If we > make a hole in it, the embryo explodes. Can anyone help? > Thanks > Caroline > > Caroline Stott > > Histology Service Unit > Medical School > University of Otago > Dunedin > (03) 479 7152 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From bill501 <@t> mindspring.com Tue Nov 25 09:18:03 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage In-Reply-To: References: Message-ID: At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant >South African, but by "contamination" do you mean leakage of waste >water? And if your controls are as strict as I am led to believe (no >oozing blood and guts or toxic chemicals going down the drain) why >does this constitute a problem?. I am afraid that I am missing >something simple here. >Unless, could it be that there are more crawling infants in >histolabs in the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org From lao_ji <@t> yahoo.com Tue Nov 25 09:28:07 2003 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] In situ hybridization In-Reply-To: Message-ID: <20031125152807.75229.qmail@web12508.mail.yahoo.com> Hi, Carlene Zindi, It seems you are going to do In situ hbridization on slides. I would like to answer your question based on this point with my experience. 1. We used dig conjugated RNA probes like fgf8, otx2, Shh and so on for mouse embryo and the brain, limbs from bigger than E12.5 mouse. They work fine. 2. The fix method is that 4% paraformaldehyde in PBS at 4 C overnight with gentl nutation. Wash in PBS 5 min at room temperature for two times. embed in OCT crystat section 10-14um thickness. 3. About 1Kb anti-sense probe is reasonable, and it is better using 3' UTR(untranslation region) due to not much conserved. Hope this help. Zhimin Lao --- "Carlene L. Zindl" wrote: > Hello, > > Does anyone have experience with non-radioactive in > situ hybridization of > frozen mouse tissue?? If so, I would appreciate > advice on details such > as: 1) is biotin or dig better? 2) what is the best > way to fix this > tissue? 3) what is the optimal size RNA probe? > > Thank you for any pertinent information. > Sincerely, > Carlene Zindl > University of Alabama at Birmingham > clzindl@artsci.wustl.edu -OR- clzindl@uab.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From StarkusL <@t> ummhc.org Tue Nov 25 09:35:01 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: Bill, that's the best explanation of the inspection process I've ever heard. -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: Tuesday, November 25, 2003 10:18 AM To: histonet@lists.utsouthwestern.edu Cc: louise renton Subject: [Histonet] RE: undersink storage At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant >South African, but by "contamination" do you mean leakage of waste >water? And if your controls are as strict as I am led to believe (no >oozing blood and guts or toxic chemicals going down the drain) why >does this constitute a problem?. I am afraid that I am missing >something simple here. >Unless, could it be that there are more crawling infants in >histolabs in the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> gold.tc.umn.edu Tue Nov 25 09:41:40 2003 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage In-Reply-To: Message-ID: <5.2.0.9.0.20031125094052.00a763b0@ander093.email.umn.edu> Bill.....couldn't have put it better myself!!!! GREAT explanation! LOL At 10:35 AM 11/25/03 -0500, Starkus, Laurie wrote: >Bill, that's the best explanation of the inspection process I've ever heard. > >-----Original Message----- >From: Bill Blank [mailto:bill501@mindspring.com] >Sent: Tuesday, November 25, 2003 10:18 AM >To: histonet@lists.utsouthwestern.edu >Cc: louise renton >Subject: [Histonet] RE: undersink storage > > >At 7:43 AM +0000 11/25/03, louise renton wrote: > >Could someone please expand on this? Obviously I am an ignorant > >South African, but by "contamination" do you mean leakage of waste > >water? And if your controls are as strict as I am led to believe (no > >oozing blood and guts or toxic chemicals going down the drain) why > >does this constitute a problem?. I am afraid that I am missing > >something simple here. > >Unless, could it be that there are more crawling infants in > >histolabs in the USA than I know of :>)!!!!!? > >GRIN. > >Hi Louise. In the US, the inspection process of hospitals and labs >has become all about power and control and not about lab reliability >or safety. > >The people in charge of 'inspecting' must find problems to justify >their existence. There is much conflict of interest in this process. >It is in a large part designed by lawyers to feed lawyers and by >people who think we all have no common sense and must be treated like >children. > >This year its about sinks; one year it's about using outdated >expensive reagents that still work; one year it was about carpets in >offices in labs, another about wearing non-dextrous rubber to prevent >needle or scalpel sticks. > >One learns to grin and bear it and to pick one's nose when being >condescended to. > >One learns to create appearances and to laugh at the inanities in life. > >One sometimes gets po'd and yells at inspectors. > >Cheers, > >Bill > >-- >_____________________________ >Bill Blank, MD >http://kernunnos.com (Celtic studies and numismatics) >http://www.druidry.org > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhadley <@t> iupui.edu Tue Nov 25 09:41:38 2003 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: I couldn't agree more!! What a waste of time and resources. Lee Ann Baldridge IUSM Indpls.,IN. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Starkus, Laurie Sent: Tuesday, November 25, 2003 10:35 AM To: 'Bill Blank'; histonet@lists.utsouthwestern.edu Cc: louise renton Subject: RE: [Histonet] RE: undersink storage Bill, that's the best explanation of the inspection process I've ever heard. -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: Tuesday, November 25, 2003 10:18 AM To: histonet@lists.utsouthwestern.edu Cc: louise renton Subject: [Histonet] RE: undersink storage At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant >South African, but by "contamination" do you mean leakage of waste >water? And if your controls are as strict as I am led to believe (no >oozing blood and guts or toxic chemicals going down the drain) why >does this constitute a problem?. I am afraid that I am missing >something simple here. >Unless, could it be that there are more crawling infants in >histolabs in the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Nov 25 10:21:25 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] restaining of hematoxylin and eosin tissue sections Message-ID: You never got rid of the mountant. The mountant used on the tape is activated when it comes in contact with xylene. So by putting the slides back in xylene, you only reactivated it. After you remove the coverslip, you must alternately rinse in water and dip in fresh acetone( not the one you used to take the covers. off). The endpoint of this step is easy to see because the mountant will turn white when it comes in contact with the water. Keep dipping and rinsing until all the white gunk is gone. Good luck! Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Peter Smale [mailto:peter.smale@nhls.ac.za] Sent: Tue 11/25/2003 2:17 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] restaining of hematoxylin and eosin tissue sections I wonder if someone can assist me in solving this problem.I retrieved a hematoxylin and eosin/phloxine stained slide from 1998 and discovered it was faded.The tissue section was mounted in a coverslipping machine that uses coverslipping tape composed of cellulose triacetate and is coated on one side with resinous mountant.This is the procedure I followed to decoverslip,decolorise and restain: I placed the slide in acetone for 7 minutes to remove the coverslip and then put the slide in xylene for 6 hours to remove any residual resinous mountant.I took the slide through graded ethanols to water after which I decolorised with acid/alcohol for one minute, took the slide back to water and restained the nuclei with Gill's Hematoxylin ( for 5 min. ) and counterstained with a mixture of 2% eosin y and 1% phloxine B with 4 drops of conc. glacial acetic acid added ( for 3 min. ).The nuclear staining turned out pale and the counter stain was patchy almost as if something was blocking the tissue's ability to retain the dyes I cannot figure out why the tissue is not staining with the same intensity as it did in 1998.Please keep in mind the slide was left out in natural light for a while which resulted in it becoming faded. ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg, South Africa tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- From tpmorken <@t> labvision.com Tue Nov 25 10:35:08 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD7E@usca0082k03.rallansci.apogent.com> Bill, great synopsis of the inspection process, but you forgot the primary method CAP uses to determine how things should be done: include inspection questions that are so vague that no one knows what they mean and then see how many ways people deal with it. Then pick one solution out of a hat and insist everyone use that solution. Tim Morken -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: Tuesday, November 25, 2003 7:18 AM To: histonet@lists.utsouthwestern.edu Cc: louise renton Subject: [Histonet] RE: undersink storage At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant >South African, but by "contamination" do you mean leakage of waste >water? And if your controls are as strict as I am led to believe (no >oozing blood and guts or toxic chemicals going down the drain) why >does this constitute a problem?. I am afraid that I am missing >something simple here. >Unless, could it be that there are more crawling infants in >histolabs in the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From StarkusL <@t> ummhc.org Tue Nov 25 10:37:24 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: And don't forget that all the other answers which were not drawn out of the hat are now WRONG so they go in and fail them in the inspection. -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Tuesday, November 25, 2003 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: undersink storage Bill, great synopsis of the inspection process, but you forgot the primary method CAP uses to determine how things should be done: include inspection questions that are so vague that no one knows what they mean and then see how many ways people deal with it. Then pick one solution out of a hat and insist everyone use that solution. Tim Morken -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: Tuesday, November 25, 2003 7:18 AM To: histonet@lists.utsouthwestern.edu Cc: louise renton Subject: [Histonet] RE: undersink storage At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant >South African, but by "contamination" do you mean leakage of waste >water? And if your controls are as strict as I am led to believe (no >oozing blood and guts or toxic chemicals going down the drain) why >does this constitute a problem?. I am afraid that I am missing >something simple here. >Unless, could it be that there are more crawling infants in >histolabs in the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchung <@t> ppmh.org Tue Nov 25 10:34:23 2003 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Slides and cassettes printer/labeler Message-ID: Histoneter, At our lab, we are looking into the slides and cassettes printer/labeler. Can anyone give me any advise or suggestion? I know that Sakura and Leica make them. Is there any other one? Pros and Cons between the two would be nice? Thanks in advance, Bruce Chung, MSM, CT(ASCP) Phoebe Putney Memorial Hospital Anatomic Pathology Manager HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. From jincan <@t> itsa.ucsf.edu Tue Nov 25 10:44:01 2003 From: jincan <@t> itsa.ucsf.edu (jincan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re: DAB fading In-Reply-To: <3FC25AE3.85D23995@sask.usask.ca> Message-ID: <5.1.1.6.0.20031125083950.00b72e40@itsa.ucsf.edu> Dear Dave, To my experience, it is more likely caused by pH's of your mounting media, as fading occurred within a few hours. Both acidic and basic condition could cause DAB color to change/fade. James Guo Lab Manager ImmunoVision Technologies, Co. 100 North Hill Drive, #32 Brisbane, CA 94005 Tel: 650-302-4622 Fax: 650-558-9621 Website: www.immunovisiontech.com At 01:24 PM 11/24/2003 -0600, you wrote: >Our experience with DAB is that it will fade slowly on >exposure to light. Are your slides in close proximity to >any flourescent light sources? The amount of fading can >also be related to the source of DAB. We have found the DAB >from Electron Microscopy Sciences to be the best for our >purposes. > >Brian Chelack >Prairie Diagnostic Services > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Nov 25 11:09:08 2003 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline ?) Message-ID: Hi Sue, Our formalin fixed specimens are brought to us by an OR runner. The fresh specimens are sent by a tube system. Our hospital is around 235 beds. Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: O'Brien, Sue [mailto:histo@bthosp.com] Sent: Tuesday, November 25, 2003 7:48 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline?) I was just wondering how other Histology Labs receive their specimens from the Operating Room (OR). Also, how do the specimens come to the Lab? (Do Histology personnel pick up the specimens? Or do OR personnel or a separate transport service bring them?) If you could include how many beds your facility has as well, I'd appreciate it. Thanks, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 e-mail: histo@bthosp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital From tpmorken <@t> labvision.com Tue Nov 25 11:20:34 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Steve Machin?? Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD81@usca0082k03.rallansci.apogent.com> Steve, you posted this awhile ago, but the link (doesn't work anymore. Can you post a link that work? Date: 27 Mar 2002 03:16:09 -0600 From: "Steve Machin UK" Subject: MSDS and Safety Assessments on a new lab site A new biomedical science site has been set up where users submit their own protocols to be downloaded by other users. http://www.greyzone.freeserve.co.uk I have put our labs COSHH assessments (safety assessments for hazardous substances) on it, about 600 substances and prepared reagents in total in MS Word format. These are linked by two MS Excel index files. The zipped file expands into about 17Mb. If you download it please ignore the file called "a_master_index.doc"; I should not have included it. Best Wishes Steve Machin UK Tim Morken Lab Vision / Neomarkers www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031125/f2eda4c5/attachment.htm From garygill <@t> dcla.com Tue Nov 25 11:24:52 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: The vagueness to which Tim alludes is widespread. Many years ago, the American Society of Cytopathology offered a voluntary lab accreditation program that included guidelines that neither the inspectors nor inspectees understood in concrete terms. I proposed abstracting pertinent portions of documents from authoritative organizations (e.g., UL, NFPA, National Safety Council, American Conference of Governmental Hygienists, etc.) and incorporating them into guidelines that were specific and understandable by all. The proposal was shot down. One then-prominent pathologist is reported to have said something to the effect: "I don't want to be held accountable to anything Gary Gill says." Talk about missing the point! Gary Gill -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Tuesday, November 25, 2003 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: undersink storage Bill, great synopsis of the inspection process, but you forgot the primary method CAP uses to determine how things should be done: include inspection questions that are so vague that no one knows what they mean and then see how many ways people deal with it. Then pick one solution out of a hat and insist everyone use that solution. Tim Morken -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: Tuesday, November 25, 2003 7:18 AM To: histonet@lists.utsouthwestern.edu Cc: louise renton Subject: [Histonet] RE: undersink storage At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant >South African, but by "contamination" do you mean leakage of waste >water? And if your controls are as strict as I am led to believe (no >oozing blood and guts or toxic chemicals going down the drain) why >does this constitute a problem?. I am afraid that I am missing >something simple here. >Unless, could it be that there are more crawling infants in >histolabs in the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Nov 25 11:45:04 2003 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] CAP survey Message-ID: Did anyone else find the summary results of the last CAP IHC survey (MKB 2003 survey) disturbing? I noticed that almost 1/3 of respondents scored the first case as Her2neu positive, when it was a 0-1+ negative case. There was also a significant portion of respondents on the lymphoma cases that could not render a diagnosis. I just wondered what others in the field thought of these results. Patti Loykasek PhenoPath Laboratories From naje1972 <@t> yahoo.com Tue Nov 25 12:36:37 2003 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] online courses or correspodence courses for the completion of Bachelor degree Message-ID: <20031125183637.40818.qmail@web41713.mail.yahoo.com> Dear Fellower Histonetters, Hi, I heard of a school call Thomas Edison for histotechnology , and that they offer courses for histologist like myself, who would like to finsh their degrees either online or by correspondence. If there is any one out in Histo-Land that knows of this school please post the information to the Histonet. Thanks in advance. Cynthia Haynes H.T. __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From StarkusL <@t> ummhc.org Tue Nov 25 12:42:39 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] online courses or correspodence courses for the co mpletion of Bachelor degree Message-ID: http://www.tesc.edu/prospective/undergraduate/credit/licenses.php#health A google search brings up the above website -----Original Message----- From: cynthia haynes [mailto:naje1972@yahoo.com] Sent: Tuesday, November 25, 2003 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] online courses or correspodence courses for the completion of Bachelor degree Dear Fellower Histonetters, Hi, I heard of a school call Thomas Edison for histotechnology , and that they offer courses for histologist like myself, who would like to finsh their degrees either online or by correspondence. If there is any one out in Histo-Land that knows of this school please post the information to the Histonet. Thanks in advance. Cynthia Haynes H.T. __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From scoop <@t> mail.nih.gov Tue Nov 25 15:16:24 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] fixing bone marrow for IHC Message-ID: Does anyone have an opinion on the best way to fix bone marrow smears for immunohistochemistry? So far I've heard methanol, acetone, B-5 and formalin. The antibodies I plan to use all work on formalin fixed paraffin embedded tissue. I'd appreciate any advice or opinions. Thanks, Sharon From Rcartun <@t> harthosp.org Tue Nov 25 15:56:06 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] fixing bone marrow for IHC Message-ID: We use formalin. I hate to see anyone use B5 because of environmental concerns with mercury. Richard Cartun Director, Immunopathology Hartford Hospital >>> Sharon Cooperman 11/25/03 04:16PM >>> Does anyone have an opinion on the best way to fix bone marrow smears for immunohistochemistry? So far I've heard methanol, acetone, B-5 and formalin. The antibodies I plan to use all work on formalin fixed paraffin embedded tissue. I'd appreciate any advice or opinions. Thanks, Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gillian.Barlow <@t> cshs.org Tue Nov 25 16:03:07 2003 From: Gillian.Barlow <@t> cshs.org (Barlow, Gillian) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Heart sections Message-ID: <3CFAA0108952D111A5BF00805FA6FB0F07947316@PEDSNTAS.csmc.edu> Dear histonetters This is the latest installment in our ongoing heart saga. I previously wrote regarding the trashed appearance of paraffin-embedded heart sections I had prepared, a problem we did not have with commercial sections from Novagen. The degree of fixation did not appear to be the problem, as H&Es on the same sections were fine, I thought it was something in our IHC protocol after dewaxing. To solve this, I did an experimet where I dewaxed 8 or 9 slides and put them through the IHC protocol, removing one slide after each step and staining with hematoxylin and eosin to check for integrity of the cellular structures. Bottom line is, all of the slides looked fine up until the streptavidin peroxidase step, after which the slides again looked chewed. We use biotinylated secondaries and ready-to-go streptavidin peroxidase from Zymed. We incubate with streptavidin peroxidase for 10 mins at room temperature. Can anyone suggest what I might be doing wrong and why we dont see this with commercially prepared slides? Same results with new and old streptavidin peroxidase so it has not "gone off". Many thanks Gillian Gillian Barlow, PhD Postdoctoral Fellow Laboratory of Julie Korenberg, PhD, MD Cedars-Sinai Medical Center Davis Bldg, Lab 2007 110 George Burns Rd Los Angeles, CA 90048 Phone: (310) 423 7650 Fax: (310) 423 0302 -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: application/ms-tnef Size: 2355 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031125/53dfb2e7/attachment.bin From mvermehren <@t> web.de Tue Nov 25 05:54:51 2003 From: mvermehren <@t> web.de (Margarete Vermehren) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] ISH on frozen tissue Message-ID: <200311251154.hAPBspQ02982@mailgate5.cinetic.de> An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031125/8184764c/attachment.htm From am102 <@t> st-andrews.ac.uk Tue Nov 25 11:33:50 2003 From: am102 <@t> st-andrews.ac.uk (Anne-Sophie Martinez) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] LOST OF ANTIGENICITY Message-ID: <200311251726.RAA24115@bute.st-andrews.ac.uk> Hello everybody! I do immunofluorescence with an AB against one aquaporine (AQP - water channel) and mdrA (multi-drug resistance protein) on some fish tissues fixed in paraformaldehyde or Bouin, embedded in paraffin and even sometimes cut (1 or 3 years ago!!). I can't manage to have any fluorescence even with very high concentrations of AB. Do you think that it can be due to the age of the tissues? Can the tissues loose the antigenicity when they have been embedded and/or cut for such a long time? Does anybody have an idea of an other possibility? Many thanks. Anne-Sophie ---------------------------------------------- Dr Anne-Sophie Martinez School of Biology University of St. Andrews Bute Medical Buildings St. Andrews Fife, KY16 9TS UK. Tel + 44 (0) 1334 463546 Fax + 44 (0) 1334 463600 From jkiernan <@t> uwo.ca Wed Nov 26 00:14:46 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re: "chewed" Heart sections (Long) References: <3CFAA0108952D111A5BF00805FA6FB0F07947316@PEDSNTAS.csmc.edu> Message-ID: <3FC444D6.C39D12C3@uwo.ca> "Barlow, Gillian" wrote: > This is the latest installment in our ongoing heart saga. I previously > wrote regarding the trashed appearance of paraffin-embedded heart sections ... > Bottom line is, all of the slides looked fine up until the > streptavidin peroxidase step, after which the slides again looked chewed.... > Can anyone suggest what I might be doing wrong and why we dont see this with commercially prepared slides? ... ______________________________________ Dear Gillian, You have carried out a well controlled trial to find the cause of your "chewed" immunostained sections. Here are a few more suggested lines of enquiry. Did you process your own and the commercially prepared slides side by side in this experiment? If you did, and only your sections were damaged by the streptavidin-HTP step, there is probably something wrong with way your sections are mounted on the slides. Possible faults include: (a) multiple tiny bubbles under the sections, due to drying the mounted ribbons while horizontal, before draining most of the water used to float out the ribbons. (b) poor adhesion to the glass, due to either a lousy adhesive or unclean glass slides. If you didn't do yours & bought slides together, you should do so. If the sections on the commercial slides are also damaged by the streptavidin-HRP solution, try exposing a few sections to this reagent alone, omitting all the other steps of the method, and see if they are damaged. If streptavidin-HRP alone causes damage to both types of section, there must be something wrong with that solution. The most probable cause is that it its pH is far too high. Check it with a recently (= same day) calibrated pH meter. Check also all buffers recently made in the lab, using bought buffers (with sound provenances) to check the calibration of the pH meter. If your pH meter responds slowly when its electrode is moved from one standard buffer to another (with intervening rinses in pure water), the electrode is probably dead. Buy a new one. pH electrodes are expensive and short-lived. The meter itself is unlikely to fail; it's electrically and electronically a quite simple device. Don't be persuaded to buy a new $1000s pH meter when you need a new $100s electrode. End of diversion. If streptavidin-HRP alone does not cause damage it's possible that the sections are not adequately fixed and securely mounted, and damage occurs in strep-HRP because they have already passed through several potentially section-detaching steps (slightly alkaline) and the strep-HRP was the last straw. A comparison of your slides with the commercially supplied ones could sort out that possibility. You are tackling the problem intelligently, using the classical approach of hypothesis and experiment. It's a method that never completely fails, but it often raises new questions. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ------------------------- From peter.smale <@t> nhls.ac.za Wed Nov 26 01:13:07 2003 From: peter.smale <@t> nhls.ac.za (Peter Smale) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] restaining of hematoxylin and eosin tissue sections References: Message-ID: <001901c3b3ec$bf786ca0$af06a8c0@PeterSmale> To Juan Gutierrez from San Antonio TX Thanks for your help.What you said makes perfect sense.You see, when we bought this coverslipper, the company that sold it to us didn't give us all the info on how to decoverslip, except that the slides must be put in acetone first. Regards ..................................................... Peter Smale Anatomical Pathology National Health Laboratory Service Chris Hani Baragwanath Hospital Soweto, Johannesburg tel: +2711 4898711 fax: +2711 4898717 e-mail: peter.smale@nhls.ac.za This message is for the designated recipient only and may contain priviliged, prorietary, or otherwise privare information. If you have received it in error, please notify the sender immediately and delete the original. Any other use of the email by you is prohibited. ----------------------------------------------------- ----- Original Message ----- From: "GUTIERREZ, JUAN" To: "Peter Smale" ; Sent: Tuesday, November 25, 2003 6:21 PM Subject: RE: [Histonet] restaining of hematoxylin and eosin tissue sections > You never got rid of the mountant. The mountant used on the tape is > activated when it comes in contact with xylene. So by putting the slides > back in xylene, you only reactivated it. After you remove the > coverslip, you must alternately rinse in water and dip in fresh acetone( > not the one you used to take the covers. off). The endpoint of this > step is easy to see because the mountant will turn white when it comes > in contact with the water. Keep dipping and rinsing until all the white > gunk is gone. Good luck! > > Juan C. Gutierrez, HT(ASCP) > Histology Supervisor > Christus Santa Rosa Healthcare > San Antonio, TX 78207 > (210)704-2533 > > -----Original Message----- > From: Peter Smale [mailto:peter.smale@nhls.ac.za] > Sent: Tue 11/25/2003 2:17 AM > To: histonet@lists.utsouthwestern.edu > Cc: > Subject: [Histonet] restaining of hematoxylin and eosin tissue sections > > > I wonder if someone can assist me in solving this problem.I retrieved a > hematoxylin and eosin/phloxine stained slide from 1998 and discovered it > was faded.The tissue section was mounted in a coverslipping machine that > uses coverslipping tape composed of cellulose triacetate and is coated > on one side with resinous mountant.This is the procedure I followed to > decoverslip,decolorise and restain: I placed the slide in acetone for 7 > minutes to remove the coverslip and then put the slide in xylene for 6 > hours to remove any residual resinous mountant.I took the slide through > graded ethanols to water after which I decolorised with acid/alcohol for > one minute, took the slide back to water and restained the nuclei with > Gill's Hematoxylin ( for 5 min. ) and counterstained with a mixture of > 2% eosin y and 1% phloxine B with 4 drops of conc. glacial acetic acid > added ( for 3 min. ).The nuclear staining turned out pale and the > counter stain was patchy almost as if something was blocking the > tissue's ability to retain the dyes I cannot figure out why the tissue > is not staining with the same intensity as it did in 1998.Please keep in > mind the slide was left out in natural light for a while which resulted > in it becoming faded. > ..................................................... > Peter Smale > Anatomical Pathology > National Health Laboratory Service > Chris Hani Baragwanath Hospital > Soweto, Johannesburg, South Africa > tel: +2711 4898711 > fax: +2711 4898717 > e-mail: peter.smale@nhls.ac.za > > This message is for the designated recipient only and may contain > priviliged, > prorietary, or otherwise privare information. If you have received it in > error, > please notify the sender immediately and delete the original. > Any other use of the email by you is prohibited. > ----------------------------------------------------- > > From louise_renton <@t> hotmail.com Wed Nov 26 02:01:59 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] RE: undersink storage Message-ID: Dear Bill & Gary Superb! You have restored my conviction that central controls are not necessarily as good as their instigators think they should be! The scary thing is that we, in this country, are not immune to rules & regs like this either, just a little tardier in setting them up. Ah well, "Onwards through the fog!" as someone else once said. Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: Bill Blank To: histonet@lists.utsouthwestern.edu CC: "louise renton" Subject: [Histonet] RE: undersink storage Date: Tue, 25 Nov 2003 09:18:03 -0600 At 7:43 AM +0000 11/25/03, louise renton wrote: >Could someone please expand on this? Obviously I am an ignorant South >African, but by "contamination" do you mean leakage of waste water? And if >your controls are as strict as I am led to believe (no oozing blood and >guts or toxic chemicals going down the drain) why does this constitute a >problem?. I am afraid that I am missing something simple here. >Unless, could it be that there are more crawling infants in histolabs in >the USA than I know of :>)!!!!!? GRIN. Hi Louise. In the US, the inspection process of hospitals and labs has become all about power and control and not about lab reliability or safety. The people in charge of 'inspecting' must find problems to justify their existence. There is much conflict of interest in this process. It is in a large part designed by lawyers to feed lawyers and by people who think we all have no common sense and must be treated like children. This year its about sinks; one year it's about using outdated expensive reagents that still work; one year it was about carpets in offices in labs, another about wearing non-dextrous rubber to prevent needle or scalpel sticks. One learns to grin and bear it and to pick one's nose when being condescended to. One learns to create appearances and to laugh at the inanities in life. One sometimes gets po'd and yells at inspectors. Cheers, Bill -- _____________________________ Bill Blank, MD http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org _________________________________________________________________ Chat with up to 14 people simultaneously - download Messenger today! http://messenger.msn.co.za/Feature/Communicate.aspx From GAshton <@t> PICR.man.ac.uk Wed Nov 26 02:36:45 2003 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] eosinophils Message-ID: Dear all, Could anybody please give me some advice on the staining of eosinophils, either by conventional stains or immuno. Am I right in thinking the carbol chromotrope technique is one such method. Many thanks in advance. Garry Ashton PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the Christie Hospital NHS Trust. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/aa4518bf/attachment.htm From jocelyn.mora <@t> anatomy.otago.ac.nz Tue Nov 25 18:33:06 2003 From: jocelyn.mora <@t> anatomy.otago.ac.nz (Jocelyn Mora) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Caspase-3, antigen retrieval Message-ID: -- Hi, I am having trouble keeping human tissue on slides undergoing antigen retrieval during Caspase-3 immunohistochemistry. The antigen retrieval involves boiling the slides in a 0.1M Tris-HCl buffer for 20mins in the microwave, which is causing the tissue to fall off the slides! I have tried wrapping slides with gauze, and using different coated slides, but so far no luck. I am looking for a different method of antigen retrieval other than the above, or treating the tissue with trypsin which is too harsh. Any suggestions would be most welcome!! Thank-you, Jocelyn Mora From Ngilman <@t> nbhd.org Wed Nov 26 08:53:23 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Autopsy duties Message-ID: I have a question for all the hospital based histo personnel, but first some background: Our facility employ a gentleman as a lab aide who makes cassettes and accessions surgical specimens. He also is trained and does the work of an autopsy technician, assisting the pathologist at autopsy. None of my histotechs are trained to do this, even though watching an autopsy and assisting at them was part of their training to get their ACP. When our lab aid is off, our PA does the post. The question came up :what if the lab aid & the PA is off and an autopsy comes up, what then? What does the rest of the world do? Thanks for your input. Have a happy Thanksgiving holiday Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/bd56369d/attachment.htm From Rita.Humphrey <@t> chsys.org Wed Nov 26 09:10:51 2003 From: Rita.Humphrey <@t> chsys.org (Rita Humphrey) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Radioactive substance Message-ID: Would anyone please share your procedure for handling sentinel nodes removed in surgery that have been treated with radioactive substance? Thank you, Rita Humphrey From lao_ji <@t> yahoo.com Wed Nov 26 09:16:23 2003 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re: in situ In-Reply-To: <3FC4B9B3.A815FAFE@erasmusmc.nl> Message-ID: <20031126151623.92392.qmail@web12507.mail.yahoo.com> Robbert Rottier, Yes, I did. It is hard to judge your how highly expressed mRNA, which Maybe I also only can detect. It is reasonable something not high enough to be detected. For example, our experiement is to electroperate some gene expression vector to one side of some embryo brain (this is chick), after some while, we detect the change of some other gene expression. mightbe the control side no detectable sign and the experiement side has. and there is timing gene expression. Your question beyond my ability. If you can overcome this problem, please kindly let me know. Zhimin Lao --- "R. rottier" wrote: > Dear Zhimin Lao, > > > I read your comments zbout in situs on slides in the > histonet list. Have > you tried to do is situs with DIG probes on paraffin > embedded material? > Our experience is that only highly expressed mRNAs > can be detected. > > What is your experience?? > > Sincerely, > > Robbert Rottier __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From ian.montgomery <@t> bio.gla.ac.uk Wed Nov 26 09:49:03 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:16 2005 Subject: Fwd: [Histonet] eosinophils Message-ID: <6.0.0.22.2.20031126154548.02d1bd80@udcf.gla.ac.uk> Garry, In my hands it's mouse, Sirius red and all other species carbol chromotrope. Let me know if you need the methods and or references. Ian. >Dear all, >Could anybody please give me some advice on the staining of eosinophils, >either by conventional stains or immuno. >Am I right in thinking the carbol chromotrope technique is one such method. >Many thanks in advance. >Garry Ashton > > >PICR >UK > >---------- >This email is confidential and intended solely for the use of the >person(s) ('the intended recipient') to whom it was addressed. Any views >or opinions presented are solely those of the author and do not >necessarily represent those of the Paterson Institute for Cancer Research >or the Christie Hospital NHS Trust. It may contain information that is >privileged & confidential within the meaning of applicable law. >Accordingly any dissemination, distribution, copying, or other use of this >message, or any of its contents, by any person other than the intended >recipient may constitute a breach of civil or criminal law and is strictly >prohibited. If you are NOT the intended recipient please contact the >sender and dispose of this e-mail as soon as possible. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From jkiernan <@t> uwo.ca Wed Nov 26 09:59:01 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] eosinophils References: Message-ID: <3FC4CDC5.43156F8C@uwo.ca> Eosinophil granules contain protein with many arginine side chains. These are strong bases (positively charged even in an alkaline environment) so they are selectively stained by anionic dyes at high pH. You may see some erythrocyte staining at ph 8, but at pH 9 the only stained objects are the cytoplasmic granules of eosinophils and Paneth cells and the tails of spermatozoa. Any anionic dye that is not decolorized at high pH is suitable. Eosin gave its name to the cells. Biebrich scarlet is good because its colour stays the same over a wide range of pH. See SS Spicer & RD Lillie 1961 Stain Technol. 36:365-370 for a thorough account of staining with alkaline solutions of anionic dyes. Remember that alkaline solutions can remove sections from slides! -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ > Garry Ashton wrote: > > Dear all, > Could anybody please give me some advice on the > staining of eosinophils, either by conventional > stains or immuno. > Am I right in thinking the carbol chromotrope > technique is one such method. > Many thanks in advance. > Garry Ashton > Is this nonsense (below) really necessary? > > PICR > UK > ----------------------------------------------------- > This email is confidential and intended solely for > the use of the person(s) ('the intended recipient') > to whom it was addressed. Any views or opinions > presented are solely those of the author and do not > necessarily represent those of the Paterson Institute > for Cancer Research or the Christie Hospital NHS > Trust. It may contain information that is privileged > & confidential within the meaning of applicable law. > Accordingly any dissemination, distribution, copying, > or other use of this message, or any of its contents, > by any person other than the intended recipient may > constitute a breach of civil or criminal law and is > strictly prohibited. If you are NOT the intended > recipient please contact the sender and dispose of > this e-mail as soon as possible. From georgecole <@t> ev1.net Wed Nov 26 10:16:34 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] messages? Message-ID: <000001c3b438$ad04ff60$014dbad0@hppav> To My Fellow Histotechs; I have to send messages on the histonet with the spelling control off.----Is this a message from the B E Y O N D??? That stubborn spelling control keeps changing histotech to hemstitch. georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/a11d69f7/attachment.htm From dellav <@t> musc.edu Wed Nov 26 10:16:37 2003 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Autopsy duties Message-ID: If your back up (your PA) should happen to be out at the same time as your primary, is your pathologist not capable of doing an autopsy?? I don't wish to sound "ugly" here but how often is this likely to happen and are not all surgical pathologists trained to perform autopsies? so where is the problem? My last question Noreen is not intended for you but rather the individual in your facility who seems to think that you don't have enough back up plans in place. Happy Thanksgiving to you Noreen Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Noreen Gilman" 11/26/03 09:53AM >>> I have a question for all the hospital based histo personnel, but first some background: Our facility employ a gentleman as a lab aide who makes cassettes and accessions surgical specimens. He also is trained and does the work of an autopsy technician, assisting the pathologist at autopsy. None of my histotechs are trained to do this, even though watching an autopsy and assisting at them was part of their training to get their ACP. When our lab aid is off, our PA does the post. The question came up :what if the lab aid & the PA is off and an autopsy comes up, what then? What does the rest of the world do? Thanks for your input. Have a happy Thanksgiving holiday Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** From MinHan.Tan <@t> vai.org Wed Nov 26 11:10:58 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Double immunostaining Message-ID: <74D0F0AB07F2E647A02D839ED79520F97B9BC9@VAIEXCH02.vai.org> Thanks all for the very considered answers to my last question. 1. I would like to enquire - I am planning on using rhodamine and FITC to double stain my tissue slides with anti-p21 and anti-p27. Unfortunately, both are nuclear proteins, and I have heard horror stories from a couple of colleagues on the difficulties of staining the same structure with different dyes. According to them, usually only one dye is predominant. Does anyone have any experiences attempting to stain the nucleus with two different dyes, or would the recommended approach be to stain two adjacent sections? 2. I have noted an apparent artefact during my ABC-DAB immunohistochemical staining of tissue. The tissue border stains heavily (an obvious artefact), but just below the border, there is a rim which completely doesn't stain at all, in stark contrast to the centre of the section. Has anyone else experienced this? Thank you! Min-Han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/980b17f4/attachment.htm From lao_ji <@t> yahoo.com Wed Nov 26 11:20:28 2003 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] In situ hybridization In-Reply-To: <3FC39832.8AB332FD@bms.com> Message-ID: <20031126172028.36967.qmail@web12508.mail.yahoo.com> Susan Wells, This is exactly a set of four books. ISBN: 047150338x I searched half.com, there are new and used book of this item there. new one cost $647.30, the used one $100 but only volumn 1&2. I couldn't recommend our Lab wibsite due to under construction. but for in situ protocol, you can visit:http://www.rodentia.com/wmc/docs/Big_In_Situ.html I found it is similar to the one I used. Hope this help. Zhimin Lao --- Susan Q Wells wrote: > A few days ago I saw a post from you regarding a > book I'm having > trouble finding. > Current protocols in molecular biology > volume2.edited by > Frederick Ausubel et al.Printed by John Wiley&sons > inc.Could you > check the book again.I cannot find anything on this > book and I am > intersetd in ordering it.Perhaps Frederick Ausubel > wrote it or I > have the title wrong? > Thanks, > Susan Wells HT,QIHC(ASCP) > > Jimmy Lao wrote: > > > Hi, Carlene Zindi, > > It seems you are going to do In situ hbridization > on > > slides. I would like to answer your question based > on > > this point with my experience. > > 1. We used dig conjugated RNA probes like fgf8, > otx2, > > Shh and so on for mouse embryo and the brain, > limbs > > from bigger than E12.5 mouse. They work fine. > > 2. The fix method is that 4% paraformaldehyde in > PBS > > at 4 C overnight with gentl nutation. Wash in PBS > 5 > > min at room temperature for two times. embed in > OCT > > crystat section 10-14um thickness. > > 3. About 1Kb anti-sense probe is reasonable, and > it is > > better using 3' UTR(untranslation region) due to > not > > much conserved. > > Hope this help. > > Zhimin Lao > > > > --- "Carlene L. Zindl" > > wrote: > > > Hello, > > > > > > Does anyone have experience with non-radioactive > in > > > situ hybridization of > > > frozen mouse tissue?? If so, I would appreciate > > > advice on details such > > > as: 1) is biotin or dig better? 2) what is the > best > > > way to fix this > > > tissue? 3) what is the optimal size RNA probe? > > > > > > Thank you for any pertinent information. > > > Sincerely, > > > Carlene Zindl > > > University of Alabama at Birmingham > > > clzindl@artsci.wustl.edu -OR- clzindl@uab.edu > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > __________________________________ > > Do you Yahoo!? > > Free Pop-Up Blocker - Get it now > > http://companion.yahoo.com/ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From sebres <@t> comcast.net Wed Nov 26 11:25:52 2003 From: sebres <@t> comcast.net (sebres) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] In situ hybridization References: <20031126172028.36967.qmail@web12508.mail.yahoo.com> Message-ID: <000601c3b442$5757a9f0$0300a8c0@homepc1> Here is an excellent web site on ISH: http://intramural.nimh.nih.gov/lcmr/snge/Protocols/ISHH/ISHH.html ----- Original Message ----- From: "Jimmy Lao" To: Sent: Wednesday, November 26, 2003 12:20 PM Subject: Re: [Histonet] In situ hybridization > Susan Wells, > This is exactly a set of four books. > ISBN: 047150338x > I searched half.com, there are new and used book of > this item there. new one cost $647.30, the used one > $100 but only volumn 1&2. > I couldn't recommend our Lab wibsite due to under > construction. but for in situ protocol, you can > visit:http://www.rodentia.com/wmc/docs/Big_In_Situ.html > I found it is similar to the one I used. > Hope this help. > Zhimin Lao > > --- Susan Q Wells wrote: > > A few days ago I saw a post from you regarding a > > book I'm having > > trouble finding. > > Current protocols in molecular biology > > volume2.edited by > > Frederick Ausubel et al.Printed by John Wiley&sons > > inc.Could you > > check the book again.I cannot find anything on this > > book and I am > > intersetd in ordering it.Perhaps Frederick Ausubel > > wrote it or I > > have the title wrong? > > Thanks, > > Susan Wells HT,QIHC(ASCP) > > > > Jimmy Lao wrote: > > > > > Hi, Carlene Zindi, > > > It seems you are going to do In situ hbridization > > on > > > slides. I would like to answer your question based > > on > > > this point with my experience. > > > 1. We used dig conjugated RNA probes like fgf8, > > otx2, > > > Shh and so on for mouse embryo and the brain, > > limbs > > > from bigger than E12.5 mouse. They work fine. > > > 2. The fix method is that 4% paraformaldehyde in > > PBS > > > at 4 C overnight with gentl nutation. Wash in PBS > > 5 > > > min at room temperature for two times. embed in > > OCT > > > crystat section 10-14um thickness. > > > 3. About 1Kb anti-sense probe is reasonable, and > > it is > > > better using 3' UTR(untranslation region) due to > > not > > > much conserved. > > > Hope this help. > > > Zhimin Lao > > > > > > --- "Carlene L. Zindl" > > > wrote: > > > > Hello, > > > > > > > > Does anyone have experience with non-radioactive > > in > > > > situ hybridization of > > > > frozen mouse tissue?? If so, I would appreciate > > > > advice on details such > > > > as: 1) is biotin or dig better? 2) what is the > > best > > > > way to fix this > > > > tissue? 3) what is the optimal size RNA probe? > > > > > > > > Thank you for any pertinent information. > > > > Sincerely, > > > > Carlene Zindl > > > > University of Alabama at Birmingham > > > > clzindl@artsci.wustl.edu -OR- clzindl@uab.edu > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > __________________________________ > > > Do you Yahoo!? > > > Free Pop-Up Blocker - Get it now > > > http://companion.yahoo.com/ > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > __________________________________ > Do you Yahoo!? > Free Pop-Up Blocker - Get it now > http://companion.yahoo.com/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Nov 26 12:05:18 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline ?) Message-ID: <90092A4ED388D7119575006008F7112049CB45@NT_EXCHANGE> O.R. puts the routine specimens in formalin and we pick them up ourselves. They have offered to bring them to us but this way we can get them when we want them. They bring anything else (frozens, lymphomas, etc.) to us fresh. We are about 225 beds. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: O'Brien, Sue [mailto:histo@bthosp.com] Sent: Tuesday, November 25, 2003 8:48 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline?) I was just wondering how other Histology Labs receive their specimens from the Operating Room (OR). Also, how do the specimens come to the Lab? (Do Histology personnel pick up the specimens? Or do OR personnel or a separate transport service bring them?) If you could include how many beds your facility has as well, I'd appreciate it. Thanks, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 e-mail: histo@bthosp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Wed Nov 26 12:31:26 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Tissue Tek VIP Processor Message-ID: <20031126.103210.5792.684306@webmail23.nyc.untd.com> Hi Histonetters, Happy Thanksgiving everyone. Does anyone know approximately how much a VIP processor is running these days? I am trying to compare to a microwave processor. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From tpmorken <@t> labvision.com Wed Nov 26 13:08:01 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Old jounal "Pathologist"? Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CD93@usca0082k03.rallansci.apogent.com> Does anyone have access to an old journal, now defunct, called "Pathologist?" If so I am looking for an article entitled: SMA/PMA Position: safe disposal of laboratory wastes containing sodium azide Pathologist. 1980 Jul;34(7):337-9 Tim Morken Lab Vision / Neomarkers www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/432a10fa/attachment.htm From garygill <@t> dcla.com Wed Nov 26 13:39:21 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Old journal "Pathologist"? Message-ID: Tim: Available through PubMed's Loansome Doc, with which service I am a registered user. If I were to order it (through Ruth Lilly Medical Library here in Indianapolis), it would cost $10. If you frequently need full-text reprints, you may want to subscribe to the service ( http://www.nlm.nih.gov/loansomedoc/loansome_home.html). Reprints as pdf files that you can save are emailed to you, usually within 1-2 days. You only pay for the reprints you order. There is no annual fee. Really a good deal. Gary Gill -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Wednesday, November 26, 2003 2:08 PM To: Histonet (E-mail) Subject: [Histonet] Old jounal "Pathologist"? Does anyone have access to an old journal, now defunct, called "Pathologist?" If so I am looking for an article entitled: SMA/PMA Position: safe disposal of laboratory wastes containing sodium azide Pathologist. 1980 Jul;34(7):337-9 Tim Morken Lab Vision / Neomarkers www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/76e32d08/attachment.htm From naje1972 <@t> yahoo.com Wed Nov 26 13:53:21 2003 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] online courses at Thomas Edison Message-ID: <20031126195321.67984.qmail@web41705.mail.yahoo.com> Thanks all; I found the information that I needed. Goggle was a big help. Sincerly yours Cynthia Haynes H.T. __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From rbeuer <@t> pacific.net.sg Wed Nov 26 15:29:49 2003 From: rbeuer <@t> pacific.net.sg (Roger Beuerman) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] HPV Message-ID: -- I have a research lab but was recently asked to process for frozen sections and immunohistochemistry, a conjunctival wart that is suspected of having HPV. This is a most common virus and many people have it on the surface of the eye. In addition to alcohol or dilute clorox cleaning of the knife blade would there be other worries and to what lengths would you gp for protection. Thank you in advance. I read the e-mails from this group daily and they have been very informative. Roger Beuerman Singapore Eye Research Institute From LuckG <@t> empirehealth.org Wed Nov 26 16:51:46 2003 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline ?) Message-ID: Below is how we function as well. We have 18 OR suites and the hospital size is just shy of 300 beds. Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 509.473.7077 luckg@empirehealth.org -----Original Message----- From: Vicki Gauch [mailto:GauchV@mail.amc.edu] Sent: Tuesday, November 25, 2003 6:49 AM To: histo@bthosp.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Specimen submission from OR (Formalin/Fresh/Saline?) We receive our OR specimens fresh and they are brought to the Frozen Section Room located in the OR by OR personnel. Our hospital has apprximately 630 beds. Vicki Gauch AMCH Albany, NY >>> "O'Brien, Sue" 11/25/03 8:47:32 AM >>> I was just wondering how other Histology Labs receive their specimens from the Operating Room (OR). Also, how do the specimens come to the Lab? (Do Histology personnel pick up the specimens? Or do OR personnel or a separate transport service bring them?) If you could include how many beds your facility has as well, I'd appreciate it. Thanks, Sue O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 e-mail: histo@bthosp.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lowman034 <@t> yahoo.com Wed Nov 26 22:00:56 2003 From: lowman034 <@t> yahoo.com (Dave Low) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Radioactive substance In-Reply-To: Message-ID: <20031127040056.58461.qmail@web40904.mail.yahoo.com> Rita, I found this in the histonet archives, I hope this answers your questions. http://www.histosearch.com/histonet/Feb02/Sentinelnodeprocedureredu.html Dave Low HT(ASCP)QIHC --- Rita Humphrey wrote: > Would anyone please share your procedure for > handling sentinel nodes removed > in surgery that have been treated with radioactive > substance? Thank you, > Rita Humphrey > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From RSRICHMOND <@t> aol.com Wed Nov 26 22:46:59 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re: Radioactive substance Message-ID: <153.276a5982.2cf6dbc3@aol.com> Rita Humphrey asks: >>Would anyone please share your procedure for handling sentinel nodes removed in surgery that have been treated with radioactive substance?<< We've discussed this subject on Histonet several times, though not very recently. Sentinel lymph nodes are labeled with technetium 99m sulfur colloid. The amount of radioactive material present in the lymph nodes - or in the subsequent lumpectomy specimen - does not require any special handling beyond the usual precautions. Radiation safety people may impose restrictions, but from an viewpoint of actual safety they are unnecessary. Technetium 99m has a half-life of six hours. It emits a gamma particle and becomes technetium 99, which has a half life on the order of hundreds of thousands of years. The minute amount of it present is not considered hazardous. To put it very crudely: you could eat the specimen and nothing much would happen to you from the radioactivity. Bob Richmond Samurai Pathologist Knoxville TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031126/7ecd535c/attachment.htm From Laurie.Reilly <@t> jcu.edu.au Wed Nov 26 22:49:31 2003 From: Laurie.Reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] eosinophils In-Reply-To: Message-ID: <5.1.0.14.0.20031127140651.00a28020@mail.jcu.edu.au> Dear Garry and All, We have used the method of Dominici (1902) as quoted in Peter Gray's Encyclopaedia to demonstrate eosinophils. It uses an aqueous solution containing 0.5% Eosin Y and 0.5% Orange G for 7 minutes, rinse in water then counterstain with 0.5% aqueous Toluidine Blue O for 20seconds. The other simple way to make eosinophils stand out is to do a H+E stain but overstain with Eosin and then differentiate the eosin until other structures, such as muscle or collagen, are very pale pink and the the eosinophils will be prominent. The Eosin solution we use contains Eosin Y and Erythrosin B. Eosinophils are very satisfying to stain, particularly horse eosinophils. Regards, Laurie. At 08:36 AM 11/26/03 +0000, Garry Ashton wrote: >Dear all, >Could anybody please give me some advice on the staining of eosinophils, >either by conventional stains or immuno. >Am I right in thinking the carbol chromotrope technique is one such method. >Many thanks in advance. >Garry Ashton > > >PICR >UK > >---------- >This email is confidential and intended solely for the use of the >person(s) ('the intended recipient') to whom it was addressed. Any views >or opinions presented are solely those of the author and do not >necessarily represent those of the Paterson Institute for Cancer Research >or the Christie Hospital NHS Trust. It may contain information that is >privileged & confidential within the meaning of applicable law. >Accordingly any dissemination, distribution, copying, or other use of this >message, or any of its contents, by any person other than the intended >recipient may constitute a breach of civil or criminal law and is strictly >prohibited. If you are NOT the intended recipient please contact the >sender and dispose of this e-mail as soon as possible. Mr.Laurie Reilly Ph 07 4781 4468 Physiology & Pharmacology Fax 07 4779 1526 Aust.Inst.of Tropical Vet.& Animal Sc. James Cook University Townsville Qld. 4811 laurie.reilly@jcu.edu.au Australia. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031127/fcd3d29e/attachment.htm From jkiernan <@t> uwo.ca Wed Nov 26 23:37:36 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re: Radioactive substance References: <153.276a5982.2cf6dbc3@aol.com> Message-ID: <3FC58DA0.8FAD551@uwo.ca> I checked Bob Richmond's half-life figures in Lange's Handbook of Chemistry. As expected, Bob R. is spot-on. It is wonderful that such low levels of radioactivity can be detected, measured, and used to estimate future surgical intervention. It's also good to know that the specimens are not dangerously radioactive by the technetium. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ____________________________ RSRICHMOND@aol.com wrote: > > Rita Humphrey asks: >>Would anyone please share your procedure for handling sentinel nodes removed in surgery that have been treated with radioactive substance?<< > > We've discussed this subject on Histonet several times, though not very recently. Sentinel lymph nodes are labeled with technetium 99m sulfur colloid. The amount of radioactive material present in the lymph nodes - or in the subsequent lumpectomy specimen - does not require any special handling beyond the usual precautions. Radiation safety people may impose restrictions, but from an viewpoint of actual safety they are unnecessary. > > Technetium 99m has a half-life of six hours. It emits a gamma particle and becomes technetium 99, which has a half life on the order of hundreds of thousands of years. The minute amount of it present is not considered hazardous. > > To put it very crudely: you could eat the specimen and nothing much would happen to you from the radioactivity. > > Bob Richmond > Samurai Pathologist > Knoxville TN From Laurie.Reilly <@t> jcu.edu.au Thu Nov 27 00:23:44 2003 From: Laurie.Reilly <@t> jcu.edu.au (Laurie Reilly) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Seep macrophage marker Message-ID: <5.1.0.14.0.20031127162039.00a34640@mail.jcu.edu.au> Dear All, Does anyone have experience in immuno staining of macrophages in sheep tissues? Any help would be appreciated. Regards and thanks, Laurie. Mr.Laurie Reilly Ph 07 4781 4468 Physiology & Pharmacology Fax 07 4779 1526 Aust.Inst.of Tropical Vet.& Animal Sc. James Cook University Townsville Qld. 4811 laurie.reilly@jcu.edu.au Australia. From stoinski <@t> molecular-machines.com Thu Nov 27 06:13:27 2003 From: stoinski <@t> molecular-machines.com (Edda Stoinski) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Ideas needed Message-ID: <3FC5EA67.9090408@molecular-machines.com> Dear Histonetters, I seach a way to transfer old archivated section from the glass slide to an other. I now there a lot of things there made this in situations you do?nt need. I search for Ideas to made this without totaly damaging the fine struktures. Thank you all Edda. From louise_renton <@t> hotmail.com Thu Nov 27 08:09:58 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Ideas needed Message-ID: Edda, This depends why you want to transfer it. If it is because the slide is broken, then you can decoverslip it, and reassemble the pieces on another slide using mounting media to stick it down and recoverslip. Not beautiful, but it works. If you want the section detached for some other reason, I recall seeing an article that used an adhesive (I think) called Diatex. Someone on histonet has this information,( if memory serves it was a gentleman either in Finland or with a Finnish surname) as I asked for the reference a while ago. Unfortunately, I've had a big cleanup, and so threw it away. This substance is used to detach cytology smears so that they can be subdivided for different stains. Hopefully someone else has more details Regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: Edda Stoinski To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ideas needed Date: Thu, 27 Nov 2003 13:13:27 +0100 Dear Histonetters, I seach a way to transfer old archivated section from the glass slide to an other. I now there a lot of things there made this in situations you doŽnt need. I search for Ideas to made this without totaly damaging the fine struktures. Thank you all Edda. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Into gaming? Challenge online buddies to the game of your choice! http://messenger.msn.co.za/Resource/Games.aspx From hodges420 <@t> msn.com Thu Nov 27 10:30:14 2003 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] fixing bone marrow for IHC Message-ID: Good Morning, I have used Methanol and acetone for smears and had great results. 15 mins... let air dry ...15 mins and let air dry. They do well even through antigen retrival. good luck Tere Hodges St Mary's Hospital Tucson,Az. >From: "Richard Cartun" >To: , >Subject: Re: [Histonet] fixing bone marrow for IHC >Date: Tue, 25 Nov 2003 16:56:06 -0500 > >We use formalin. I hate to see anyone use B5 because of environmental >concerns with mercury. > >Richard Cartun >Director, Immunopathology >Hartford Hospital > > >>> Sharon Cooperman 11/25/03 04:16PM >>> >Does anyone have an opinion on the best way to fix bone marrow smears >for immunohistochemistry? So far I've heard methanol, acetone, B-5 >and formalin. The antibodies I plan to use all work on formalin >fixed paraffin embedded tissue. I'd appreciate any advice or >opinions. > >Thanks, >Sharon > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ online games and music with a high-speed Internet connection! Prices start at less than $1 a day average. https://broadband.msn.com (Prices may vary by service area.) From hodges420 <@t> msn.com Thu Nov 27 10:37:50 2003 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] LOST OF ANTIGENICITY Message-ID: good morning, Yes antigenicity can be lost on slides. I recommend that you cut 25 slides test the first and last then keep a log . When the stainging starts to decrease, that will tell you the life of your slide antigenicity. Temp and climate effect slides in a lab as well as fixation and processing of specimens. Thats one reason controls are to be obtained from with in one's lab. If labs send out immunos,if or ish control tissue should be sent from the lab to the reference to be completely accurate for the test to be correct. Good luck Tere Hodges St. Mary Hospital Tucson, Az >From: am102@st-andrews.ac.uk (Anne-Sophie Martinez) >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] LOST OF ANTIGENICITY >Date: Tue, 25 Nov 2003 17:33:50 +0000 > >Hello everybody! > >I do immunofluorescence with an AB against one aquaporine (AQP - water >channel) and mdrA (multi-drug resistance protein) on some fish tissues >fixed in paraformaldehyde or Bouin, embedded in paraffin and even sometimes >cut (1 or 3 years ago!!). >I can't manage to have any fluorescence even with very high concentrations >of AB. Do you think that it can be due to the age of the tissues? Can the >tissues loose the antigenicity when they have been embedded and/or cut for >such a long time? Does anybody have an idea of an other possibility? >Many thanks. > >Anne-Sophie > >---------------------------------------------- >Dr Anne-Sophie Martinez >School of Biology >University of St. Andrews >Bute Medical Buildings >St. Andrews >Fife, KY16 9TS >UK. > >Tel + 44 (0) 1334 463546 >Fax + 44 (0) 1334 463600 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Has one of the new viruses infected your computer? Find out with a FREE online computer virus scan from McAfee. Take the FreeScan now! http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From cycling <@t> another.com Thu Nov 27 12:03:34 2003 From: cycling <@t> another.com (cycling@another.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re:Old sections, and BM ISH/IHC Message-ID: <4344402.1069956214315.JavaMail.root@172.16.100.50> Excuse me, my first note and the system will not let me open the mail to use to reply, anyway We found that the heat mediated epitope retrieval largely obviated problems with sections that had been hanging around for months, being reviewed etc, or archival blocks fron ten or more years ago Bone marrow, well a couple of thoughts and questions. Fixing smears for IHC has been 30 mins in Neutral Buffered Formalin, could look for the cytology paper that said as much. So we used the same for any Bone marrows we get, once in a blue moon. ISH, no experience, but.. I am looking for thoughts on BM Trephine biopsies. When we do ISH then the results appear much weaker than the tonsil or other tissue that runs alongside. That is whether we are enzyme or heat mediated pretreatments. Any ideas? Trephines are EDTA decalcified. IHC is no problem David Edmondson Histopath Christie Hospital Manchester UK -- Personalised email by http://another.com From hamdi <@t> doctor.com Thu Nov 27 15:14:02 2003 From: hamdi <@t> doctor.com (hamdi abuali) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Transfecting GFP gene into cells in suspension Message-ID: <20031127211402.2956.qmail@mail.com> Hello All Currently I am trying to transfect umbilical cord cells with GFP gene , the cells are not cultures, they are in suspension , and I do not want to culture them becuase I am studying their maturity level and I dont want any factors to promote differentiation . I would like to know if anyone has a protocol in that regard . Thank you Hamdi Abu-Ali, MD. =-=-=-=-==-==-=- Hamdi Abu-Ali , MD. University of South Florida Cardiology - Tampa, Florida -- ___________________________________________________________ Sign-up for Ads Free at Mail.com http://promo.mail.com/adsfreejump.htm From carolynluty <@t> hotmail.com Thu Nov 27 18:50:45 2003 From: carolynluty <@t> hotmail.com (Carolyn Luty) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] MOHS Message-ID: I am having problems with the epidermis sometimes folding when I stain . Can anyone give me some input ?? I stain with Toluidine Blue. _________________________________________________________________ The new MSN 8: advanced junk mail protection and 2 months FREE* http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From robinmax <@t> rogers.com Thu Nov 27 19:06:15 2003 From: robinmax <@t> rogers.com (Robin Maxwell-Nunn) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Movat's pentachrome stain Message-ID: <000e01c3b54b$d2b35a90$0c117218@GOODCOMPOOTER> Our lab is setting up a Movat's pentachrome staining method. We have tried several similar methods and with all of them we are having problems with differentiating the background (greenish-blue) stain. Can anyone give any insight as to what might be the problem i.e. longer differentiation with 5% phosphomolybdic acid? thanks, Robin St Mary's General Hospital, Kitchener, Ontario. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031127/ff7f6c08/attachment.htm From Delventh3 <@t> aol.com Thu Nov 27 19:48:46 2003 From: Delventh3 <@t> aol.com (Delventh3@aol.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] used books Message-ID: <15f.28f78afb.2cf8037e@aol.com> Hello Histonetters: In response to the info about finding used books. I found a neat site for such needs; try addall.com Priscilla - Still working, but in Las Cruces, NM. One more week here. It's a beautiful city Felitz Navidad -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031127/3641b5c7/attachment.htm From juan.gutierrez <@t> christushealth.org Fri Nov 28 10:59:01 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Autopsy duties Message-ID: I have three Histotechs, including myself, that can perform autopsies. Juan C. Gutierrez, HT(ASCP) Histology Suoervisor -----Original Message----- From: Noreen Gilman [mailto:Ngilman@nbhd.org] Sent: Wed 11/26/2003 8:53 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Autopsy duties I have a question for all the hospital based histo personnel, but first some background: Our facility employ a gentleman as a lab aide who makes cassettes and accessions surgical specimens. He also is trained and does the work of an autopsy technician, assisting the pathologist at autopsy. None of my histotechs are trained to do this, even though watching an autopsy and assisting at them was part of their training to get their ACP. When our lab aid is off, our PA does the post. The question came up :what if the lab aid & the PA is off and an autopsy comes up, what then? What does the rest of the world do? Thanks for your input. Have a happy Thanksgiving holiday Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** From siksik03 <@t> comcast.net Fri Nov 28 12:10:36 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Tissue Tek VIP Processor In-Reply-To: <20031126.103210.5792.684306@webmail23.nyc.untd.com> References: <20031126.103210.5792.684306@webmail23.nyc.untd.com> Message-ID: Hi HistoNetters Marsha Price asked about comparing the price of a traditional overnight tissue processor (a VIP) with a microwave processor. There are several points to consider when making such a calculation: 1) Reagent costs: A traditional overnight tissue processor can have as many as 14 reagent stations with a capacity of several liters each. These chemicals include fixative, graded series of alcohols, xylene or other clearing agent and wax. All of them need to be rotated and changed on a fixed schedule. A microwave processor uses only four reagents (fixative, 100% ethanol or reagent alcohol, isopropanol and wax), usually in quantities of 3 liters or less. Because xylene is not used in the microwave, there are no xylene disposal or recycling costs associated with microwave processing. Also, because the xylene does not contaminate the paraffin, the wax can be used essentially forever, cutting down significantly on wax consumption. 2) Labor costs No one needs to spend the time to clean rotate and change the chemicals on the microwave processor. I know this is everyone's favorite job on the traditional tissue processor ! 3) Efficiency There is no need for a "rinse" cycle on a microwave processor between biopsy runs. Runs can be made in immediate succession without any downtime. 4) Service costs I would never recommend buying a traditional tissue processor without a service contract. Pumping systems require maintenance. Microwave tissue processors have few moving parts and require essentially no service. So, in addition to the upfront savings, there are ongoing savings with a microwave that need to be considered as well. best regards, Steven Slap (still recovering from an overabundance of food yesterday) At 6:31 PM +0000 11/26/03, mprice26@juno.com wrote: >Hi Histonetters, >Happy Thanksgiving everyone. > >Does anyone know approximately how much a VIP processor is running >these days? I am trying to compare to a microwave processor. > >Marsha Price > From am102 <@t> st-andrews.ac.uk Sat Nov 29 04:35:35 2003 From: am102 <@t> st-andrews.ac.uk (Anne-Sophie Martinez) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Fixations Message-ID: <200311291027.KAA09454@bute.st-andrews.ac.uk> Hello, Could anybody please help me. I would like to know what is the best fixation for IHC experiments (4% PFA or Bouin?), classical electron microscopy (I am planning 2.5% glutaraldehyde in sodium cacodylate buffer) and immunogold in electron microscopy (I am planning 0.5% glutaraldehyde in sodium cacodylate buffer)? Is LR-white the best resin for immunogold staining? Thank you very much for your help. Anne-Sophie ---------------------------------------------- Dr Anne-Sophie Martinez School of Biology University of St. Andrews Bute Medical Buildings St. Andrews Fife, KY16 9TS UK. Tel + 44 (0) 1334 463546 Fax + 44 (0) 1334 463600 From Sellis4051 <@t> aol.com Sat Nov 29 16:25:29 2003 From: Sellis4051 <@t> aol.com (Sellis4051@aol.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Re: Autopsy Duties Message-ID: <1d8.158ab405.2cfa76d9@aol.com> I'm a histotech who's worked a couple different autopsy jobs and I've got a couple suggestions for morgue coverage when both the PA and deiner are off duty. 1. Two pathologists work together 2. Pathologist lowers expectations and uses anyone willing to stand there and hold/move/clean stuff. 3. Cultivate an autopsy volunteer pool. Plenty of people claim to be interested in morgue work and are looking for ways to break into the field. Any of the above three suggestions should bring respect for the missing PA and diener who may even enjoy the challenge of creating a training checklist for these volunteers. At my most recent job there was a "second stringer" for morgue work who would fill in if the "main man" was unavailable and they never had the same days off. I enjoy assisting at autopsy so much that I come in on my days off to be there. Fascinating work if you can get it as there is always something to learn from each case. Sandra Ellis HT-ASCP Diplomat-American Board of Medicolegal Death Investigation Message: 13 Date: Wed, 26 Nov 2003 09:53:23 -0500 From: "Noreen Gilman" To: Subject: [Histonet] Autopsy duties I have a question for all the hospital based histo personnel, but first some background: Our facility employ a gentleman as a lab aide who makes cassettes and accessions surgical specimens. He also is trained and does the work of an autopsy technician, assisting the pathologist at autopsy. None of my histotechs are trained to do this, even though watching an autopsy and assisting at them was part of their training to get their ACP. When our lab aid is off, our PA does the post. The question came up :what if the lab aid & the PA is off and an autopsy comes up, what then? What does the rest of the world do? Thanks for your input. Have a happy Thanksgiving holiday Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031129/61900276/attachment.htm From rkerschmann <@t> yahoo.com Sun Nov 30 08:27:21 2003 From: rkerschmann <@t> yahoo.com (Russ Kerschmann) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] H&E to PAS restaining protocol Message-ID: <20031130142721.35453.qmail@web20212.mail.yahoo.com> Hello Histonet, Can anyone provide a protocol for reprocessing an H&E slide into a PAS slide? Can the reverse also be performed? How can the risk of losing the tissue be minimized? Many thanks, and Happy Holidays. Russell Kerschmann, M.D. rkerschmann@yahoo.com --------------------------------- Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031130/e2c09e0e/attachment.htm From jkiernan <@t> uwo.ca Sun Nov 30 22:03:51 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] H&E to PAS restaining protocol References: <20031130142721.35453.qmail@web20212.mail.yahoo.com> Message-ID: <3FCABDA7.9F9B60A9@uwo.ca> Russ Kerschmann MD wrote: > Can anyone provide a protocol for reprocessing an H&E > slide into a PAS slide? Can the reverse also be > performed? Easy! Here's a procedure. I'm not happy about calling this kind of thing a "protocol" because it needs observation and judgement if it's to work properly. You should do this job yourself; do not entrust it to someone else. 1. Remove the coverslip and mountant and take the section to 70% alcohol. Don't rush any step of this procedure, and be especially certain that you have dissolved out all the mounting medium. 2. Immerse in acid-alcohol until all the haemalum nuclear (and any other blue) staining has gone. 3. Rinse in 70% alcohol, then in water. 4. Put in slightly alkaline water until the eosin colour (pink) has all gone. (Note. You will probably not be able to extract eosin from a few strongly basic materials present in some animal tissues: eosinophils, Paneth cells and spermatozoa.) 5. Wash in tap water, then in distilled water before doing the PAS method. >How can the risk of losing the tissue be > minimized? Keep exposure to alkalinity (Step 4 above) to a minimum. Hard tap water may be sufficient, or you can add a small drop of ammonium hydroxide, or of saturated aqueous lithium carbonate. High pH removes sections from slides. > Many thanks, and Happy Holidays. Which holiday(s)? The next one on my calendar is Nobel Day (Sweden) on 10th December, followed by Our Lady of Guadalupe (Mexico & Central America) 2 days later. Chanukah isn't until the 19th. Or do you mean happy Christmas? It's still 25 days away! Hope this helps. -- ------------------------- John A. Kiernan MB, ChB Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/