From Nancy.Walker <@t> sanofi-synthelabo.com Mon Dec 1 09:10:40 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] tissue quality controls for ish and ihc Message-ID: Hello, I'm struggling with ideas about immunohistochemistry and in situ hybridization controls to make sure tissue has been not over fixed and also that the mRNA has been well preserved. I work on detecting mRNA or protein for targets for orphan receptors (most of which we have very little info where the target is. In the past working on rodent brain tissue, we got nice results "assuming" that everything was perfect. Now I'm working on human tissue arrays where I don't control the fixation and processing so I'd like to have an internal control. Even in rodent tissue I think it would be a good idea to control the tissue. I'm making a riboprobe for vWF because it's found in all tissues (low in liver gut and muscle) and I like the idea of having something specific to a particular cell type (vessel endothelial cells), compared to something like GAPDH. What do you think about an oligo dT probe? This detects poly (A) mRNA, the advantage is that it would be universal for all species. I found it in the BioGenex catelogue on page 157, it is supposedly used to assess mRNA preservation. Any comments?? For our riboprobe ISH we are thinking of making an oligo with a T7 promotor followed by a tail of T (25 or more). Then we'll transcribe with a mix of UTP's radioactive and normal (1:20) and this will hybridize to all mRNA (they have poly A tails). Biogenex also promote a monoclonal antibody (V9) to Vimentin, on page 113 (reference cited: Battifora, H., Am J Clin Pathol 96:669, 1991). I ordered the article, does anyone have it? Does any vimentin work or is this clone special??? This sounds nice does any one have any comments? yours truely, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From sthevar <@t> bu.edu Mon Dec 1 09:33:21 2003 From: sthevar <@t> bu.edu (Sandy Thevarkunnel) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] cresylechtviolett (cresyl fast violet) staining problems Message-ID: Hi, We just bought new cresyl fast violet from Cell point scientific to replace our old cresyl fast violet from Roboz which was produced in 1995. The new stain seems to wash away from the tissue when we put it in the 95% ethanol with acetic acid step rapidly, but prior to the step there does not seem to be any detectable differentiation during our 75% ETOH, 75% ETOH with acetic acid and the 95% steps that precede the 95 with acid step. Our protocol for making the cresyl fast violet is to add 2.5g to 500mL deionized H2O stir and then filter. This recipe was great with the old stain but the new does not seem to be working. We are staining formalin fixed human brainstems cut at 50um. Staining protocol we use Defatting- 3hrs in 1:1 Chloroform:Ethanol Hydration: 7 minutes- 2x 100% ETOH, 2X 95%ETOH, 1x70%ETOH 10 minutes- dH2O Staining- 4-6 minutes in CV 30 seconds dH2O Differentiation from 75%, 75% with Acetic acid, 95%, to 95% with acetic acid, variable times from 1 minute to 4 minutes Dehydration- 2x 100% ETOH for 4 minutes Clearing- 3 changes of Xylene 2min, 1min, 30 sec. Coverslip with Permount Sorry for the long message I was just hoping if it wasn't the recipe it just might be our protocol so if anyone has any ideas I'd really appreciate them, I'm kind of new at this. By the way our old CV was black but the solution was still purple and the new CV is green and solution is the same purple colour but it seems thinner. Thank you very much, Sandy Boston University School of Medicine From Stephen.Eyres <@t> sanofi-synthelabo.com Mon Dec 1 09:52:18 2003 From: Stephen.Eyres <@t> sanofi-synthelabo.com (Stephen.Eyres@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] Can anyone hear me? Message-ID: Hi, Just a test message to see if my new string works. Steve From jkiernan <@t> uwo.ca Mon Dec 1 10:16:30 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:16 2005 Subject: [Histonet] cresylechtviolett (cresyl fast violet) staining problems References: Message-ID: <3FCB695E.ED0F7E2@uwo.ca> Is your dye from a batch certified by the Biological Stain Commission? If so, it will have a little sticker on the bottle that says so, and it will probably be called cresyl violet acetate. For variations in names and identities (there's no correlation!) see Conn's Biol. Stains 10th ed. (2002), Chapter 19 (by R.W.Horobin). A certified batch of cresyl violet should give a good result if you use the same procedure that the B.S.C. uses to test the dye for certification. This is described in a paper by D.P.Penney et al. 2002 "Analysis and testing of biological stains - the Biological Stain Commission procedures." Biotech. Histochem. 77: 237-275. This paper describes the testing methods for all the 61 currently certifiable stains. If your lab doesn't get this inexpensive journal you should at least obtain Vol 77 issue 5/6 for Sep/Nov 2002, which contains the B.S.C. procedures paper. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ________________________ Sandy Thevarkunnel wrote: > > Hi, > We just bought new cresyl fast violet from Cell point scientific to > replace our old cresyl fast violet from Roboz which was produced in > 1995. The new stain seems to wash away from the tissue when we put it > in the 95% ethanol with acetic acid step rapidly, but prior to the step > there does not seem to be any detectable differentiation during our 75% > ETOH, 75% ETOH with acetic acid and the 95% steps that precede the 95 > with acid step. Our protocol for making the cresyl fast violet is to > add 2.5g to 500mL deionized H2O stir and then filter. This recipe was > great with the old stain but the new does not seem to be working. We > are staining formalin fixed human brainstems cut at 50um. > Staining protocol we use > Defatting- 3hrs in 1:1 Chloroform:Ethanol > Hydration: 7 minutes- 2x 100% ETOH, 2X 95%ETOH, 1x70%ETOH > 10 minutes- dH2O > Staining- 4-6 minutes in CV > 30 seconds dH2O > Differentiation from 75%, 75% with Acetic acid, 95%, to 95% with acetic > acid, variable times from 1 minute to 4 minutes > Dehydration- 2x 100% ETOH for 4 minutes > Clearing- 3 changes of Xylene 2min, 1min, 30 sec. > Coverslip with Permount > > Sorry for the long message I was just hoping if it wasn't the recipe it > just might be our protocol so if anyone has any ideas I'd really > appreciate them, I'm kind of new at this. By the way our old CV was > black but the solution was still purple and the new CV is green and > solution is the same purple colour but it seems thinner. > > Thank you very much, > Sandy > Boston University School of Medicine > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Mon Dec 1 10:37:55 2003 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Renal biopsy charges/procedures Message-ID: <61135F0455D33347B5AAE209B903A30403262313@EXCHVS2.medctr.ad.wfubmc.edu> We are currently looking at our charges and procedures for renal biopsies and my manager wanted me to see if anyone on the Histonet would be willing to share what procedures they do for renals (ie, special stains, immunos, EM, etc.), the usual turnaround time, and total charge (both professional and technical). Thank you in advance for any information. You may reply to me personally or post on the Histonet. Martha Ward Wake Forest University Baptist Medical Center -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031201/ebb7a20a/attachment.htm From haldana <@t> unimoron.edu.ar Mon Dec 1 10:52:00 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] cresylechtviolett (cresyl fast violet) staining problems References: Message-ID: <002401c3b82b$729cf7a0$7504a8c0@um.edu> Dear Sandy, sometimes is not necessary (depends of the quality of the stain) the adition of acetic acid to the alcohol, do the differentiation only with pure 96% alcohol. Remember to make the deshidratation in ol 100 very quiclky. The xilol no decolorate the section. Another form is: do te deshidratation in the oven al 58?C and then put the slide directly in xilol. I do it, if the alcohol decolorate very quickly my sections. Sorry for my English.... This is our modification of Burk's stain (1969). This stain is used to show Nissl bodies and cellular patterns. 1- Paraffin sections 15-20mm 2- Dewax sections in xylene, hydrate through graded ethanol to water. 3- Stain for 3 to 5 minutes in cresyl violet solution (See appendix). 4- Wash in tap or distilled water. 5- Differentiate in 96% ethanol. The differentiation must be checked under microscope, to obtain only colored Nissl bodies and nuclei. If differentiation becomes difficult, sometimes it is necessary to add some drops of 0.1% glacial acetic acid in 96% ethanol to accelerate this process. If acidified ethanol is used, wash in several changes of distilled water and then begin the dehydration by quick immersion in 96% ethanol. Differentiation may be stopped in distilled water when several slides are simultaneously being processed. 6- 100% ethanol (2 minutes), clear in xylene and mount in Eukitt. Cresyl violet solution Cresyl violet (Kresylviolett-Merck) 0.1g. Distilled water 50ml. Acetate buffer 0.2M pH 3.6 50ml. Dissolve the cresyl violet in the distilled water. Add the acetate buffer, mix the reagents and filter before using. This solution is very stable and may be used for 5 months with excellent results. From:.Biocell 1996. 20(3): 265-272. Standardization of Fixation, Processing and Staining Method for the Central Nervous System of Vertebrates.H J. ALDANA MARCOS, C.C. FERRARI, I.BENITEZ AND J. M. AFFANNI. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: "Sandy Thevarkunnel" To: Sent: Monday, December 01, 2003 12:33 PM Subject: [Histonet] cresylechtviolett (cresyl fast violet) staining problems > Hi, > We just bought new cresyl fast violet from Cell point scientific to > replace our old cresyl fast violet from Roboz which was produced in > 1995. The new stain seems to wash away from the tissue when we put it > in the 95% ethanol with acetic acid step rapidly, but prior to the step > there does not seem to be any detectable differentiation during our 75% > ETOH, 75% ETOH with acetic acid and the 95% steps that precede the 95 > with acid step. Our protocol for making the cresyl fast violet is to > add 2.5g to 500mL deionized H2O stir and then filter. This recipe was > great with the old stain but the new does not seem to be working. We > are staining formalin fixed human brainstems cut at 50um. > Staining protocol we use > Defatting- 3hrs in 1:1 Chloroform:Ethanol > Hydration: 7 minutes- 2x 100% ETOH, 2X 95%ETOH, 1x70%ETOH > 10 minutes- dH2O > Staining- 4-6 minutes in CV > 30 seconds dH2O > Differentiation from 75%, 75% with Acetic acid, 95%, to 95% with acetic > acid, variable times from 1 minute to 4 minutes > Dehydration- 2x 100% ETOH for 4 minutes > Clearing- 3 changes of Xylene 2min, 1min, 30 sec. > Coverslip with Permount > > Sorry for the long message I was just hoping if it wasn't the recipe it > just might be our protocol so if anyone has any ideas I'd really > appreciate them, I'm kind of new at this. By the way our old CV was > black but the solution was still purple and the new CV is green and > solution is the same purple colour but it seems thinner. > > Thank you very much, > Sandy > Boston University School of Medicine > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan <@t> uwo.ca Mon Dec 1 11:24:12 2003 From: jkiernan <@t> uwo.ca (J. A. Kiernan) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Biotech. Histochem. (Was cresylechtviolett ...) Message-ID: <3FCB793C.B9EE7992@uwo.ca> Sorry. In my reply about cresyl violet I should have given a link to the publisher of Biotech. Histochem. which has changed twice in recent years. Currently it's Taylor & Francis. They publish many journals; their web page for B & H is: http://www.tandf.co.uk/journals/titles/10520295.asp -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From frank <@t> purdue.edu Mon Dec 1 11:21:45 2003 From: frank <@t> purdue.edu (Frank Rosenthal) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] sectioning old GMA blocks Message-ID: Dear colleagues: Does anyone have experience in sectioning glycol methacrylate tissue blocks that are several years old? It seems difficult to get good sections from some of them because they are brittle. (I am using a Hacker microtome with a steel knife --section thickness 2 - 6 micrometer. The embedded tissue is from dried inflated lung). Any suggestions? Thanks. Frank ***************************************** Frank S. Rosenthal, Ph.D. Associate Professor Purdue University School of Health Sciences 1338 Civil Engineering Building West Lafayette, IN 47907 USA tel: 765-494-0812, fax: 765-496-1377, e-mail: frank@purdue.edu ***************************************** From thoward <@t> unm.edu Mon Dec 1 11:40:38 2003 From: thoward <@t> unm.edu (Tamara Howard) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] RE: transfecting GFP into suspension cells Message-ID: You asked about getting a GFP construct into cells growing in suspension - 2 possibilities: If you have access to the proper "toy", you can electroporate. But you'll need an electroporator and the cuvettes....and working out the conditions for a new cell line can be a royal pain. Second option - use one of the transfection reagents available on the market. FuGene and Lipofectamine are the "old standards"; check with Clontech, Roche, Gibco.... Good luck! |--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward@unm.edu |--------------------------------------------------| From CTague <@t> ahs.llumc.edu Mon Dec 1 12:30:06 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] 200 proof absolute ethyl alcohol Message-ID: i remember using some of this stuff many years ago to process tissue and ended up with a bunch of fried or burnt looking tissue. i was told ethyl alcohol is not good for processing tissue but i think i've heard different since then. anyone have any comments? can ethyl alcohol be used to process tissue and the error was a result of some other mistake? thanks for your help, curt Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From rschoon <@t> email.unc.edu Mon Dec 1 12:30:12 2003 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] sectioning old GMA blocks References: Message-ID: <3FCB88B4.9020003@email.unc.edu> GMA is hydrophilic and depending on the storage conditions will either gain or lose water (in your case lost). Depending on the brittleness of the block anywhere from 12 to 72 hours in a humidity chamber (read container with dH2O dampened gauze). Do not place the blocks directly on your water source. Robert Schoonhoven Frank Rosenthal wrote: >Dear colleagues: > > Does anyone have experience in sectioning glycol methacrylate tissue blocks >that are several years old? It seems difficult to get good sections from >some of them because they are brittle. (I am using a Hacker microtome with a >steel knife --section thickness 2 - 6 micrometer. The embedded tissue is >from dried inflated lung). > > Any suggestions? > > Thanks. > >Frank > >***************************************** >Frank S. Rosenthal, Ph.D. >Associate Professor >Purdue University School of Health Sciences >1338 Civil Engineering Building >West Lafayette, IN 47907 USA >tel: 765-494-0812, fax: 765-496-1377, >e-mail: frank@purdue.edu >***************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From pmarcum <@t> polysciences.com Mon Dec 1 12:47:36 2003 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] sectioning old GMA blocks In-Reply-To: <3FCB88B4.9020003@email.unc.edu> Message-ID: <003101c3b83b$96afa2e0$4800a8c0@PMARCUM2K> That is the best advise you will get to rehydrate the blocks. If you over hydrate the cutting will be too soft and you will need to carefully watch them in a dessicator to get the right consistency. However, Bob is right don't place them directly in water. Pam Marcum > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Robert > Schoonhoven > Sent: Monday, December 01, 2003 1:30 PM > To: Frank Rosenthal > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] sectioning old GMA blocks > > > GMA is hydrophilic and depending on the storage conditions will either > gain or lose water (in your case lost). Depending on the brittleness of > the block anywhere from 12 to 72 hours in a humidity chamber (read > container with dH2O dampened gauze). Do not place the blocks directly > on your water source. > > Robert Schoonhoven > > Frank Rosenthal wrote: > > >Dear colleagues: > > > > Does anyone have experience in sectioning glycol > methacrylate tissue blocks > >that are several years old? It seems difficult to get good > sections from > >some of them because they are brittle. (I am using a Hacker > microtome with a > >steel knife --section thickness 2 - 6 micrometer. The embedded tissue is > >from dried inflated lung). > > > > Any suggestions? > > > > Thanks. > > > >Frank > > > >***************************************** > >Frank S. Rosenthal, Ph.D. > >Associate Professor > >Purdue University School of Health Sciences > >1338 Civil Engineering Building > >West Lafayette, IN 47907 USA > >tel: 765-494-0812, fax: 765-496-1377, > >e-mail: frank@purdue.edu > >***************************************** > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From VanmetMJ <@t> pbrc.edu Mon Dec 1 13:19:36 2003 From: VanmetMJ <@t> pbrc.edu (Montina Van Meter) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] GAD 67 Message-ID: Dear Histonetters, I am looking for a GAD 67 antibody that has good cell body staining. I will be sectioning 40 micron free-floating mouse brainstem that has been fixed with 4% Paraformaldehyde and sectioned with a freezing microtome. My secondary antibody is Alexa Fluor 594. Thank you, Tina Van Meter Pennington Biomedical Research Center Baton Rouge, LA From Loralee_Gehan <@t> URMC.Rochester.edu Mon Dec 1 13:32:41 2003 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] 200 proof absolute ethyl alcohol Message-ID: <95774A6A6036D411AFEA00D0B73C86430888048F@exmc3.urmc.rochester.edu> I have never used anything but ethyl alcohol. Sounds like the time you spent in the alcohol was much too long. That usually gives you a fried up, dried up mess. Loralee Gehan University of Rochester Orthopaedics Research > ---------- > From: Tague, Curtis > Sent: Monday, December 1, 2003 1:30 PM > To: Histonet (E-mail) > Subject: [Histonet] 200 proof absolute ethyl alcohol > > i remember using some of this stuff many years ago to process tissue and > ended up with a bunch of fried or burnt looking tissue. i was told ethyl > alcohol is not good for processing tissue but i think i've heard different > since then. anyone have any comments? can ethyl alcohol be used to process > tissue and the error was a result of some other mistake? > > > thanks for your help, > curt > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains > information that may be confidential, protected by applicable legal > privileges, or constitute non-public information. It is intended to be > conveyed only to the designated recipient(s). If you are not an intended > recipient of this message, please notify the sender by replying to this > message and then delete it from your system. Use, dissemination, > distribution or reproduction of this message by unintended recipients is > not authorized and may be unlawful. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jqb7 <@t> cdc.gov Mon Dec 1 13:23:30 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] 200 proof absolute ethyl alcohol Message-ID: We use ethyl on our VIP with no problems. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Tague, Curtis Sent: Monday, December 01, 2003 1:30 PM To: Histonet (E-mail) Subject: [Histonet] 200 proof absolute ethyl alcohol i remember using some of this stuff many years ago to process tissue and ended up with a bunch of fried or burnt looking tissue. i was told ethyl alcohol is not good for processing tissue but i think i've heard different since then. anyone have any comments? can ethyl alcohol be used to process tissue and the error was a result of some other mistake? thanks for your help, curt Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Mon Dec 1 13:45:41 2003 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] H&E to PAS staining Message-ID: I've done this in the past and found that the Periodic Acid removes Hematoxylin. The eosin (most of it) was removed in the hydrating alcohols and water after removing the mounting medium. If you want absolute quality control, you might consider H&E staining your control slide and then use the destain "protocol" John Kiernan provided, but I doubt most people will go to this length. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 tjj@stowers-institute.org From scoop <@t> mail.nih.gov Mon Dec 1 14:21:11 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Transfecting GFP gene into cells in suspension In-Reply-To: <20031127211402.2956.qmail@mail.com> References: <20031127211402.2956.qmail@mail.com> Message-ID: I would suggest you try genejuice from Novagen. I get remarkably high transfection efficiencies with very little toxicity and have been able to transfect cell lines that others in my lab say are not transfectable. Sharon >Hello All > >Currently I am trying to transfect umbilical cord cells with GFP >gene , the cells are not cultures, they are in suspension , and I do >not want to culture them becuase I am studying their maturity level >and I dont want any factors to promote differentiation . > >I would like to know if anyone has a protocol in that regard . > >Thank you > >Hamdi Abu-Ali, MD. > > >=-=-=-=-==-==-=- >Hamdi Abu-Ali , MD. >University of South Florida >Cardiology - Tampa, Florida > >-- >___________________________________________________________ >Sign-up for Ads Free at Mail.com >http://promo.mail.com/adsfreejump.htm > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GrobeA <@t> saintpatrick.org Mon Dec 1 15:01:22 2003 From: GrobeA <@t> saintpatrick.org (Albert Grobe) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Trichrome Staining Intensity Message-ID: Some time ago, I found a paper that stated that the intensity of the blue color in the Masson's Trichrome stain was proportional to the amount of cross-linking, i.e. older, more cross-linked collagen was a darker blue compare to a lighter stained "newer" collagen. Does anyone have a reference that they can supply me, as I can't seem to find my paper? Albert From la.sebree <@t> hosp.wisc.edu Mon Dec 1 16:12:34 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] HIER using B & D rice steamer Message-ID: One of our pathologists is looking for an inexpensive way to antigen retrieve tissues for staining with CD3, CD68 and CD56. I suggested a Black and Decker rice steamer although I personally have no experience with one. Is anyone out there willing to share their retrieval protocol? I (and he) would greatly appreciate it. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From i_stain <@t> yahoo.com Mon Dec 1 17:28:45 2003 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Re: Double immunostaining Message-ID: <20031201232845.1244.qmail@web42003.mail.yahoo.com> Min-Han, There is some commerically available double staining kits outthere work very nice. Zymed has a kit called "Picture DS polymer kit" (not sure the exact name), you can contact them to get more details. I hope this helps Scott Message: 20 Date: Wed, 26 Nov 2003 12:10:58 -0500 From: "Tan, MinHan" To: Subject: [Histonet] Double immunostaining This is a multi-part message in MIME format. ------_=_NextPart_001_01C3B440.42BA41C3 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable Thanks all for the very considered answers to my last question.=20 =20 1. I would like to enquire - I am planning on using rhodamine and FITC to double stain my tissue slides with anti-p21 and anti-p27. Unfortunately, both are nuclear proteins, and I have heard horror stories from a couple of colleagues on the difficulties of staining the same structure with different dyes. According to them, usually only one dye is predominant. Does anyone have any experiences attempting to stain the nucleus with two different dyes, or would the recommended approach be to stain two adjacent sections? =20 2. I have noted an apparent artefact during my ABC-DAB immunohistochemical staining of tissue. The tissue border stains heavily (an obvious artefact), but just below the border, there is a rim which completely doesn't stain at all, in stark contrast to the centre of the section. Has anyone else experienced this?=20 Thank you! =20 Min-Han Tan =20 __________________________________ Do you Yahoo!? Free Pop-Up Blocker - Get it now http://companion.yahoo.com/ From jthorsten <@t> rvfarms.com Mon Dec 1 18:37:26 2003 From: jthorsten <@t> rvfarms.com (Jason Thorsten) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] H&E on PMMA ground sections Message-ID: Hi histonetters, We are embedding tissue specimens in PMMA. We are using the EXAKT system to grind the sections to approximately 25 micron sections. We are mainly working with vessels with metal implants. We have tried to stain the sections with Gills 3 Hematoxylin and Eosin Y/Phloxine. We are having problems with the nuclei taking up stain. We have tried the following pretreatments before staining to no avail: -Treatment with 50% alcohol-2 minutes -Treatment with 70% alcohol-4 minutes -Treatement with 1%HCl in water-2 minutes -Treatment of 1% Ammonium Hydroxide in water-2 minutes -Treatment with 5% Formic acid-10 minutes After pretreatment, we have placed the slides in deionized water to rinse quickly and placed into the hematoxylin. We are at a loss what to try. Can anyone offer any suggestions? Best Regards, Robert Brunner rbrunner@rvfarms.com Jason Thorsten jthorsten@rvfarms.com RVF, Inc. From RFail <@t> Charleston.net Mon Dec 1 18:32:18 2003 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] HIER using B & D rice steamer In-Reply-To: Message-ID: <002701c3b86b$bf885420$c011a6a5@rena> Linda, We place the retrieval solution in the steamer to preheat when we place the slides in xylene to deparaffinize. Use distilled water to the fill line of the steamer, Turn the dial of the steamer past 40. Use 50 ml of solution per plastic coplin jar, and place a thermometer through one of the holes in the lid into the buffer solution.The temperature reaches 99 degrees in the time it takes to hydrate the slides. We use a digital thermometer from Fisher with a thin probe(under 20 dollars). Always place the same number of slides in each coplin jar(7) even if you have to use blank slides. We put the slides in the steamer for 20 minutes, take them out of the steamer, allow the solution to cool down for 10 minutes and rinse in 3 changes of tris buffer. The most important thing is consistency. Work out the time that gives you the best results for your lab. Record the temp, when you place the slides in the solution and when you take them out. Be sure to lift the lid away from you to prevent burns. wE found the steamer is less harsh on derm tissue than a pressure cooker Rena Fail IHC/SS lab Medical University of South Carolina Charleston, SC 943-792-3384 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, December 01, 2003 5:13 PM To: Histonet E-mail Cc: Slukvin Igor I Subject: [Histonet] HIER using B & D rice steamer One of our pathologists is looking for an inexpensive way to antigen retrieve tissues for staining with CD3, CD68 and CD56. I suggested a Black and Decker rice steamer although I personally have no experience with one. Is anyone out there willing to share their retrieval protocol? I (and he) would greatly appreciate it. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tomers <@t> shaw.ca Mon Dec 1 23:27:21 2003 From: tomers <@t> shaw.ca (Tom Wells) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Nuclear staining artefact Message-ID: <001601c3b894$f6194e30$6401a8c0@laptop> It never fails, just when I start to think that I have everything worked out in Immunohistochemistry I find something new to prove that I still have a lot to learn. I have begun to see some strange nuclear staining in bone marrow biopsies. There isn't a general increase in background only the specific nuclear staining in the negative control. I have seen this before, but usually only the rare cell and only on the periphery of the tissue. Recently, however, the tissues have shown a large number of positive staining nuclei throughout the tissue. This artefact doesn't show up in the slides stained with a primary antibody only the negative control. I have never seen this in any tissue other than bone marrow biopsies. We fix our bone marrows in B5 and decalcify with RDO. Has anyone else seen this? Does anyone have a theory? Thanks. Tom Tom Wells Supervisor, Immunohistochemistry Lions Gate Hospital North Vancouver, BC Canada -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031201/5078b63d/attachment.htm From stoinski <@t> molecular-machines.com Tue Dec 2 02:29:24 2003 From: stoinski <@t> molecular-machines.com (Edda Stoinski) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] he to pas staining Message-ID: <3FCC4D64.70803@molecular-machines.com> You can remove H&E with 70% Alcohol including HCl acid. This type of HCl-Alcohol, you can order as an finish solution by the most histology-companys. PAS is not an staining it is an reaction that you can not remove only bleach a little bit. Edda Stoinski From Terry.Coaker <@t> nuth.northy.nhs.uk Tue Dec 2 03:55:28 2003 From: Terry.Coaker <@t> nuth.northy.nhs.uk (Coaker, Terry) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] unsubscribe Message-ID: Thankyou Terry Terry Coaker Histopathology Operations Manager Cellular Pathology Royal Victoria Infirmary Queen Victoria Road Newcastle upon Tyne NE1 4LP 0191 282 4445 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/aec27530/attachment.htm From TMcNemar <@t> lmhealth.org Tue Dec 2 05:11:01 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] HIER using B & D rice steamer Message-ID: <90092A4ED388D7119575006008F7112049CB46@NT_EXCHANGE> We use the B&D steamer for all retrievals. We preheat the reagent for 5 minutes then add the slides for 20 minutes. Remove them and allow them to cool for another 20 minutes. They then sit in buffer for another 5 minutes before putting them on the Dako Autostainer. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu] Sent: Monday, December 01, 2003 5:13 PM To: Histonet (E-mail) Cc: Slukvin Igor I Subject: [Histonet] HIER using B & D rice steamer One of our pathologists is looking for an inexpensive way to antigen retrieve tissues for staining with CD3, CD68 and CD56. I suggested a Black and Decker rice steamer although I personally have no experience with one. Is anyone out there willing to share their retrieval protocol? I (and he) would greatly appreciate it. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From FANDORFP <@t> aol.com Tue Dec 2 05:53:31 2003 From: FANDORFP <@t> aol.com (FANDORFP@aol.com) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] MOHS Message-ID: <104.3acd03f5.2cfdd73b@aol.com> Are your slides completely dry, before you begin staining? Or are the sections lifting during staining? If lifting during staining, there are plus slides you can use that will help prevent the sections lifting! Good Luck Lin HT -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/4ba94ab7/attachment.htm From rschoon <@t> email.unc.edu Tue Dec 2 07:26:50 2003 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] H&E on PMMA ground sections References: Message-ID: <3FCC931A.30008@email.unc.edu> I'm afraid that you are missing a critical step here.......PMMA is basically unaffected by any of the reagents mentioned. A little back ground search through the literature, admittedly sparse as there are lab's making a lot of money on stents so they are not publishing (working out the methodology is simply time and equipment intensive, the science is easy), and the HistoNet archives. I'll make my reply short and let you look up the specifics. Just use a couple of 15 minute changes of warm xylene (55-60oC). Robert Schoonhoven Jason Thorsten wrote: > Hi histonetters, > > We are embedding tissue specimens in PMMA. We are using the EXAKT > system to grind the sections to approximately 25 micron sections. We > are mainly working with vessels with metal implants. We have tried to > stain the sections with Gills 3 Hematoxylin and Eosin Y/Phloxine. We > are having problems with the nuclei taking up stain. We have tried the > following pretreatments before staining to no avail: > -Treatment with 50% alcohol-2 minutes > -Treatment with 70% alcohol-4 minutes > -Treatement with 1%HCl in water-2 minutes > -Treatment of 1% Ammonium Hydroxide in water-2 minutes > -Treatment with 5% Formic acid-10 minutes > > After pretreatment, we have placed the slides in deionized water to > rinse quickly and placed into the hematoxylin. We are at a loss what > to try. Can anyone offer any suggestions? > > Best Regards, > > Robert Brunner > rbrunner@rvfarms.com > > Jason Thorsten > jthorsten@rvfarms.com > > RVF, Inc. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> mail.vetmed.lsu.edu Tue Dec 2 08:08:35 2003 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Restaining slides Message-ID: From: "Cheryl Crowder" To: rkerschmann@yahoo.com Date: Tue, 02 Dec 2003 08:07:43 -0600 Subject: Restaining slides Good morning - An answer to your questions about restaining slides: There is no problem restainng the H&E. Remove the coverglass, place in xylene (with agitation to remove the mounting medium), the alcohols, bacl to water. Then place in 1% acid alcohol to decolorize the hematoxylin. The eosin will be removed with the water rinses. Then rinse well in water and perform the PAS. To restain the PAS is a little different. The best way is first to purchase Schiff Hand Cleaner from Anatech, Kalamazoo, MI. This is a thick liquid. Remove the coverglass from the slide, take back through xylene to water. With the slide wet mix a little of the hand cleaner with a little water to make a slurry. Apply to the tissue. Let set for several minutes. The slurry can be rinsed off and the depth of remaining staining checked. If it is still too dark repeat the process. The handcleaner will decolorize the slide. Rinse slide well with water and stain with regular H & E . Hope this helps. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 From danyl002 <@t> umn.edu Tue Dec 2 08:36:48 2003 From: danyl002 <@t> umn.edu (danyl002) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Hipoxyprobe for retina Message-ID: <200312021436.hB2Eam4a031958@challenge.software.umn.edu>  Did anyone use Hipoxyprobe staining for retina or optic nerve? Is there any other way to measure a degree of hypoxia in nerve tissue(besides actual axon counting)? From Loralee_Gehan <@t> URMC.Rochester.edu Tue Dec 2 08:38:18 2003 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] isolator/pap pens Message-ID: <95774A6A6036D411AFEA00D0B73C864308880490@exmc3.urmc.rochester.edu> Does anyone have a good source for isolator or pap pens? Besides biocare. I have had a stream on bad pens and they are not too cheap. Thanks in advance. Loralee Gehan University of Rochester Orthopaedics Research Department From tpmorken <@t> labvision.com Tue Dec 2 10:17:01 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Senior Technologist, Biotech Immunohistochemistry position openin g, east San Francisco bay area Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CDAA@usca0082k03.rallansci.apogent.com> Repeat of an earlier announcement: Lab Vision Corporation has an opening for a Senior Technologist in the Immunohistochemistry QC and Development laboratory. The successful candidate will have extensive experience in the development of novel antibodies for histology applications with emphasis on optimal labeling methodology. Extensive experience in clinical application of antibody probes to tissue pathology is desired. Additionally the position requires design input and testing of new instruments and other products. The successful candidate will be one who thrives on applying experimental procedures on a daily basis and relishes solving problems in immunohistochemistry. Relocation assistance is possible for the right candidate. Lab Vision is a leader in automation of immunohistochemistry and an innovator in antibody development, most recently with the introduction of Rabbit Monoclonal antibodies. We are also a leader in the research antibody field. We will soon introduce an extensive line of IVD antibodies for the clinical market. Lab Vision is located in Fremont, California, in the East San Francisco Bay Area. See our website at www.labvision.com Please respond to: Tim Morken Product Development Lab Vision / NeoMarkers 47790 Westinghouse Dr Fremont, CA 94539 PH: 510-991-2840 www.labvision.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/9c237bc1/attachment.htm From Luis.Chiriboga <@t> med.nyu.edu Tue Dec 2 11:22:13 2003 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Hipoxyprobe for retina In-Reply-To: <200312021436.hB2Eam4a031958@challenge.software.umn.edu> Message-ID: I have used hypoxyprobe in mice GBM model, works well but you may want to stay away from peroxidase if there's a lot of necrosis. Might also want to consider antibody against hif1alpha..... Luis -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of danyl002 Sent: Tuesday, December 02, 2003 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hipoxyprobe for retina Did anyone use Hipoxyprobe staining for retina or optic nerve? Is there any other way to measure a degree of hypoxia in nerve tissue(besides actual axon counting)? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dave_laidley <@t> yahoo.ca Tue Dec 2 11:20:47 2003 From: dave_laidley <@t> yahoo.ca (David Laidley) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] aqueous mounting medium nad refractive index Message-ID: <20031202172047.82829.qmail@web20805.mail.yahoo.com> Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums. Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available) David Laidley Memorial University of Newfoundland Division of Basic Medical Sciences St. John's, Newfoundland dave_laidley@yahoo.ca --------------------------------- Post your free ad now! Yahoo! Canada Personals -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/edff09ca/attachment.htm From mark.lewis <@t> thermo.com Tue Dec 2 11:27:11 2003 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Re: Immuno & Insitu Workshops Message-ID: Hello everyone, I'd like to know if there are any upcoming workshops for Immuno Histochemistry, In Situ or FISH. Particularly interested in any that deal with trouble shooting, but am still interested in any related workshops. Thanks ! Mark Mark Lewis From fmonson <@t> wcupa.edu Tue Dec 2 11:46:24 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] H&E to PAS restaining protocol Message-ID: Dear Russ, Back in the '60's, I routinely counterstained the PAS with H&E, the magenta of the Basic Fuchsin is not confused with the orange/red of the Eosin. Also, since I always use alcoholic Eosin, that which can be extracted from the tissue will dissolve quite nicely in subsequent rinses in 95% EtOH. Eosin tends to be less soluble in 100% EtOH than in 95%. Now, to the matter of converting an H&E to a PAS. My take on your request is that you would like to bleach an H&E, and reprocess as PAS. My working hypothesis would be as follows. Since the PAS preparations with which I am familiar are already quite acidic, I would expect any H in the section to be destabilized, to the extent possible, by the PA first and then the S themselves. Alcohols and water should take care of that part of the E that can be removed. In contrast to J. Kiernan, with whom I take exception only with great trepidation, I would use no other than the following, which I would describe as a relatively straight forward - Protocol. 1. Soak slides in Xylene to remove coverglass and excess mountant. Five slides in 25-35 degrees 'C' Xylene for four hours, tease the coverglasses away with #11 scalpel blade and then leave overnight. 2. Soak again to insure complete removal of mountant. Thus, two more fresh Xylene rinses for 8 and 16 hours respectively. 3. Bring sections slowly to water. a. Excess times in ethanols will have no deleterious effect on PAS positivity, so again rinse in several Coplin jars of 100%, then 95%, then 70% EtOH. Two to three hours should provide sufficient time in a total of six alcohols (2x100, 2x95 & 2x70) b. Any touch of milkiness in sections once they are in 70% is evidence of residual xylene. Go back to 100% and re-hydrate. 4. Immerse in 1% periodic acid for 10 min at RT. 5. My PAS uses the de'Tomasi version of the Schiff's ('S') reagent (found in Pearse, all editions). Immerse for 10 min at RT, though I have left for up to 20 min without any observable negative effect. 6. Rinse at least 2X in HOH (always d.i. or distilled). HOH should demonstrate little if any pink color, though on standing some color will appear. 7. Rinse in 'gently' running tap water (Lehigh or Delaware Valley's of Pennsylvania, if possible) for 5 min. [This step is ALL about modern alchemistery [Sic(k?)!], no matter in what procedure it is used!] The section should 'pink' (magenta?) up the section. 8. Quickly view with cover glass (HOH mounted) and green filter (Wratten #58 is my favorite). This step will help to certify the PAS and to determine advisability of re-countering with H and/or E. Unless I were running parallel sections as PAS controls, I would always counter with H&E. NOTE: I counter with H&E after I process sections or air-dried mesentery spreads in Gomori's Aldehyde Fuchsin for elastin. 9. Mount coverglass as required. Hope this helps, Fred Monson (Never known for brevity!) Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Russ Kerschmann [mailto:rkerschmann@yahoo.com] Sent: Sunday, November 30, 2003 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E to PAS restaining protocol Hello Histonet, Can anyone provide a protocol for reprocessing an H&E slide into a PAS slide? Can the reverse also be performed? How can the risk of losing the tissue be minimized? Many thanks, and Happy Holidays. Russell Kerschmann, M.D. rkerschmann@yahoo.com _____ Do you Yahoo!? Protect your identity with Yahoo! Mail AddressGuard -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/f8f3d0a2/attachment.htm From fmonson <@t> wcupa.edu Tue Dec 2 11:55:37 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] aqueous mounting medium nad refractive index Message-ID: Hi Dave, Pardon the duplication, but an answer is most efficient on a list, but attachments are not. I have sent you a PDF file of a Refractive Index summary that I put together at the beginning of 2003. Any others who would like it should request it of me by off-list email using: Subject: RI No text will be required - nor thanks since I will assume each email so received to include all that is required for me to quickly respond. Cheers, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: David Laidley [mailto:dave_laidley@yahoo.ca] Sent: Tuesday, December 02, 2003 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting medium nad refractive index Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums. Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available) David Laidley Memorial University of Newfoundland Division of Basic Medical Sciences St. John's, Newfoundland dave_laidley@yahoo.ca _____ Post your free ad now! Yahoo! Canada Personals -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/e0a43d65/attachment.htm From Barry.R.Rittman <@t> uth.tmc.edu Tue Dec 2 12:03:14 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] aqueous mounting medium nad refractive index Message-ID: <566FB0B522443D43AF02D2ADBE35A6F06358C7@UTHEVS3.mail.uthouston.edu> David If "faramount" is the same as Farrant's mountant it is around 1.425. PVA mountants can have a RI starting at approximately 1.3 and Gray's formula has a RI of 1.382 Glycerol has RI of 1.313 to 1.47 depending on its concentration (78 ml. glycerin + 22 ml. of water RI = 1.44, 1:1 glycerin:water has a RI of 1.4). There are a large number of formulae . What specifically were you interested in placing in an aqueous mountant? Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of David Laidley Sent: Tuesday, December 02, 2003 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting medium nad refractive index Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums. Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available) David Laidley Memorial University of Newfoundland Division of Basic Medical Sciences St. John's, Newfoundland dave_laidley@yahoo.ca Post your free ad now! Yahoo! Canada Personals From tpmorken <@t> labvision.com Tue Dec 2 12:18:10 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] aqueous mounting medium nad refractive index Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CDB2@usca0082k03.rallansci.apogent.com> Barry, It's not the same. "Faramount" is a DAKO product named after a person named Faranak, who worked at DAKO and developed it. I believe it is now called DAKOmount since Faranak left the company. Tim Morken Lab Vision / NeoMarkers email: tpmorken@labvision.com www.labvision.com -----Original Message----- From: Barry R Rittman [mailto:Barry.R.Rittman@uth.tmc.edu] Sent: Tuesday, December 02, 2003 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] aqueous mounting medium nad refractive index David If "faramount" is the same as Farrant's mountant it is around 1.425. PVA mountants can have a RI starting at approximately 1.3 and Gray's formula has a RI of 1.382 Glycerol has RI of 1.313 to 1.47 depending on its concentration (78 ml. glycerin + 22 ml. of water RI = 1.44, 1:1 glycerin:water has a RI of 1.4). There are a large number of formulae . What specifically were you interested in placing in an aqueous mountant? Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of David Laidley Sent: Tuesday, December 02, 2003 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting medium nad refractive index Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums. Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available) David Laidley Memorial University of Newfoundland Division of Basic Medical Sciences St. John's, Newfoundland dave_laidley@yahoo.ca Post your free ad now! Yahoo! Canada Personals _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronnie_Houston <@t> bshsi.com Tue Dec 2 12:18:10 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] consulting fee Message-ID: <530361BF03351B4CAE5270A05D3037B5FE057A@bsrexms01.BSHSIR.COM> Would anyone care to hazard an idea as to how much to charge for a technical consulting fee? This would be a long term project, involving sacrificing vacation. Would you consider a value in relation to your present hourly rate or a fixed sum per day? Thanks Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. From StarkusL <@t> ummhc.org Tue Dec 2 12:25:34 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] consulting fee Message-ID: Ronnie, I get a $50/hour with a $200 guarantee per day. This is for Mohs however. -----Original Message----- From: Houston, Ronnie [mailto:Ronnie_Houston@bshsi.com] Sent: Tuesday, December 02, 2003 1:18 PM To: Histonet (E-mail) Subject: [Histonet] consulting fee Would anyone care to hazard an idea as to how much to charge for a technical consulting fee? This would be a long term project, involving sacrificing vacation. Would you consider a value in relation to your present hourly rate or a fixed sum per day? Thanks Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com ____________________________________________________________________________ ____________________________________________________ ____________________________________________________________________________ ____________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From e.goldberg <@t> biogenex.com Tue Dec 2 13:01:26 2003 From: e.goldberg <@t> biogenex.com (Eleonora Goldberg) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] FW: PAP Pens Message-ID: Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: logo_datasheet.gif Type: image/gif Size: 1248 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/daad9215/logo_datasheet.gif -------------- next part -------------- A non-text attachment was scrubbed... Name: promotions_click.gif Type: image/gif Size: 1935 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/daad9215/promotions_click.gif -------------- next part -------------- A non-text attachment was scrubbed... Name: i6000.gif Type: image/gif Size: 1275 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/daad9215/i6000.gif -------------- next part -------------- A non-text attachment was scrubbed... Name: i6000_pic.gif Type: image/gif Size: 21182 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/daad9215/i6000_pic.gif From amosbrooks <@t> earthlink.net Tue Dec 2 13:04:25 2003 From: amosbrooks <@t> earthlink.net (amosbrooks@earthlink.net) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] HIER using B & D rice steamer Message-ID: <22611741.1070391871204.JavaMail.root@thecount.psp.pas.earthlink.net> Linda, The steamer is absolutly the best method of retrieval around. The heating is even and can be directly monitored in real time. If you drill a hole in the top of the steamer you can put a thermometer directly into the solution. The steamer is not subject to the temperature spikes you see using a microwave. The price is usually right. The steamers can usually be found for under $50.00. I highly reccommend it. If you have any questions just ask:-). Amos Brooks BTW: Be careful with the hole in the top. I have heard of someone that had a hard plastic top and it broke on him. I believe the Black & Decker has a soft plastic top (that's what I use) so it should be no problem. Message: 10 Date: Mon, 1 Dec 2003 16:12:34 -0600 From: "Sebree Linda A." To: "Histonet (E-mail)" Cc: "Slukvin Igor I" Subject: [Histonet] HIER using B & D rice steamer One of our pathologists is looking for an inexpensive way to antigen = retrieve tissues for staining with CD3, CD68 and CD56. I suggested a = Black and Decker rice steamer although I personally have no experience = with one. Is anyone out there willing to share their retrieval = protocol? I (and he) would greatly appreciate it. Thank you, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From bryand <@t> netbistro.com Tue Dec 2 13:08:47 2003 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] aqueous mounting medium nad refractive index References: <20031202172047.82829.qmail@web20805.mail.yahoo.com> Message-ID: <006901c3b907$b8b6c040$a270c2cf@bryand> By better refractive index, I presume you mean higher refractive index. If that is correct, the aqueous medium I used when I wanted good clarity was PVP glycerol. I have no reference, nor do I know who introduced it, but the recipe is: Polyvinylpyrrolidone 50 grams Distilled water 50 ml Stir the two together, and put in a 56C incubator until it is a clear syrup. Add 10 mL glycerol, mix very well. Leave another hour or so for bubbles to rise, then cool to room temperature. In a capped container it will last for years. Almost dry sections, place the medium onto the center of the section, not the edge of the slide. Apply coverslip. Do not press. Make any adjustments to the coverslip immediately as it sets in about five minutes. Its disadvantage is that it slowly dries back over some months. I used this medium for muscle biopsy enzymes and it worked very well. Bryan Llewellyn ----- Original Message ----- From: David Laidley To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 02, 2003 9:20 AM Subject: [Histonet] aqueous mounting medium nad refractive index Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums. Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available) David Laidley Memorial University of Newfoundland Division of Basic Medical Sciences St. John's, Newfoundland dave_laidley@yahoo.ca ------------------------------------------------------------------------------ Post your free ad now! Yahoo! Canada Personals -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/950a1c25/attachment.htm From MTitford <@t> aol.com Tue Dec 2 13:17:02 2003 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Histotechnology trivia Message-ID: <4B2425B9.1A9BAFAF.00762DB1@aol.com> I got a card today telling me Thermo Shandon is now Thermo Electron, the latest step in companies gobbleing each other up. Only a few years ago there was the Shandon and Lipshaw companies. Shandon took over Lipshaw, who were in turn taken over by Thermo electron. And then years ago Spencer Microtomes were taken over by American Optical, who were taken over by Reichert, and then Leica. Is that right? Will there be only one or two histology equipment companies soon? Mike Titford USA Pathology Mobile AL USA From garygill <@t> dcla.com Tue Dec 2 13:28:11 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Histotechnology trivia Message-ID: Don't forget Sakura Finetek and Richard-Allan Scientific, and SurgiPath. Gary Gill -----Original Message----- From: MTitford@aol.com [mailto:MTitford@aol.com] Sent: Tuesday, December 02, 2003 2:17 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnology trivia I got a card today telling me Thermo Shandon is now Thermo Electron, the latest step in companies gobbleing each other up. Only a few years ago there was the Shandon and Lipshaw companies. Shandon took over Lipshaw, who were in turn taken over by Thermo electron. And then years ago Spencer Microtomes were taken over by American Optical, who were taken over by Reichert, and then Leica. Is that right? Will there be only one or two histology equipment companies soon? Mike Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From haldana <@t> unimoron.edu.ar Tue Dec 2 14:17:50 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] destain "protocol" for IHC References: Message-ID: <00a501c3b911$5d716140$7504a8c0@um.edu> MessageCan anyone provide a protocol for reprocessing an immunohistochemistry? I like to bleach an immuno with DAB, and reprocess with other antibody with the same developer (DAB again) in the same slide. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/fec81ec3/attachment.htm From froyer <@t> bitstream.net Tue Dec 2 14:27:41 2003 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Histotechnology trivia In-Reply-To: <4B2425B9.1A9BAFAF.00762DB1@aol.com> References: <4B2425B9.1A9BAFAF.00762DB1@aol.com> Message-ID: <3FCCF5BD.4040801@bitstream.net> MTitford@aol.com wrote: >I got a card today telling me Thermo Shandon is now Thermo Electron, the latest step in companies gobbleing each other up. > >Only a few years ago there was the Shandon and Lipshaw companies. Shandon took over Lipshaw, who were in turn taken over by Thermo electron. > >And then years ago Spencer Microtomes were taken over by American Optical, who were taken over by Reichert, and then Leica. Is that right? Will there be only one or two histology equipment companies soon? > >Mike Titford >USA Pathology >Mobile AL USA > I hear you Mike... it's almost impossible to keep up with all the mergers, and not just in Histology. Here's a funny that relates to the topic: Investment tips for 2004.... for all of you with any money left In the wake of the Exxon/Mobile deal and the AOL/TimeWarner implode be aware of the next expected mergers so that you can get in on the ground floor and make some BIG bucks. Watch for these consolidations in 2004: 1. Hale Business Systems, Mary Kay Cosmetics, Fuller Brush, and W. R.Grace Co. will merge and become: Hale, Mary, Fuller, Grace. 2. Polygram Records, Warner Bros., and Zesta Crackers join forces and become: Poly, Warner Cracker. 3. 3M will merge with Goodyear and issue forth as: MMMGood. 4. Zippo Manufacturing, Audi Motors, Dofasco, and Dakota Mining will merge and become: ZipAudiDoDa. 5. FedEx is expected to join its major competitor, UPS, and become: FedUP. 6. Fairchild Electronics and Honeywell Computers will become: Fairwell Honeychild. 7. Grey Poupon and Docker Pants are expected to become: Poupon Pants. 8. Knotts Berry Farm and the National Organization of Women will become: Knott NOW! That's all for now.....invest wisely! Ford Royer, MT(ASCP) Analytical Instruments Minneapolis, MN From Diane.Gladney <@t> se.amedd.army.mil Tue Dec 2 14:37:10 2003 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Histotechnology trivia Message-ID: <9D41AB7C56F8304F98537ABD87B249F662616E@dasmthgbz001.amedd.army.mil> Thanks, I needed that laugh....hospital computer system has been down for 2 days and has just now been restored to operation. Lots of info to enter into LIS now.... I really needed this light hearted commentary. Swamped With Paper Work, Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: Ford Royer [mailto:froyer@bitstream.net] Sent: Tuesday, December 02, 2003 3:28 PM To: Histo Net Subject: Re: [Histonet] Histotechnology trivia MTitford@aol.com wrote: >I got a card today telling me Thermo Shandon is now Thermo Electron, the latest step in companies gobbleing each other up. > >Only a few years ago there was the Shandon and Lipshaw companies. Shandon took over Lipshaw, who were in turn taken over by Thermo electron. > >And then years ago Spencer Microtomes were taken over by American Optical, who were taken over by Reichert, and then Leica. Is that right? Will there be only one or two histology equipment companies soon? > >Mike Titford >USA Pathology >Mobile AL USA > I hear you Mike... it's almost impossible to keep up with all the mergers, and not just in Histology. Here's a funny that relates to the topic: Investment tips for 2004.... for all of you with any money left In the wake of the Exxon/Mobile deal and the AOL/TimeWarner implode be aware of the next expected mergers so that you can get in on the ground floor and make some BIG bucks. Watch for these consolidations in 2004: 1. Hale Business Systems, Mary Kay Cosmetics, Fuller Brush, and W. R.Grace Co. will merge and become: Hale, Mary, Fuller, Grace. 2. Polygram Records, Warner Bros., and Zesta Crackers join forces and become: Poly, Warner Cracker. 3. 3M will merge with Goodyear and issue forth as: MMMGood. 4. Zippo Manufacturing, Audi Motors, Dofasco, and Dakota Mining will merge and become: ZipAudiDoDa. 5. FedEx is expected to join its major competitor, UPS, and become: FedUP. 6. Fairchild Electronics and Honeywell Computers will become: Fairwell Honeychild. 7. Grey Poupon and Docker Pants are expected to become: Poupon Pants. 8. Knotts Berry Farm and the National Organization of Women will become: Knott NOW! That's all for now.....invest wisely! Ford Royer, MT(ASCP) Analytical Instruments Minneapolis, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pam <@t> ategra.com Tue Dec 2 14:51:11 2003 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Histology openings Latest Update 12/03/03 Message-ID: Hi histonetters, I hope you all had a wonderful Thanksgiving. Here is the latest update on opportunities with some of my best clients throughout the US who are seeking Histology Supervisors, Histo Technologists and Histo Technicians. These are positions as direct employees of our client. These are fulltime 40 hour per week positions. As a direct employee of one of our clients you will be provided with full benefits including Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative sign-on bonus. I have supervisory, team lead and bench positions. These positions require HTL or HT certification or registry eligibility. Here are my NEWEST openings: 1. Baltimore, MD - Histo Tech 2. Central VA - Histo Tech 3. Southern VA - Operations Manager 4. Northern, VA - Histo Tech 5. Orlando, FL - Histo Tech 6. Atlanta, GA - Histo Tech 7. Southern Florida - Histo Tech 8. Michigan - Histo Tech 9. San Antonio, TX - Histo Tech 10.Boston, MA - Histo Tech Here are some of my HOTTEST Histology Supevisory positions: 1.Maine - Histology Supervisor 2.Syracuse, NY - Histology Supervisor 3.Illinois - Team Lead 4.Pittsburgh, PA - Histology Supervisor Here are some of my HOTTEST Histo Tech bench positions: 1.Oregon - Histo Tech 2.Illinois - Team Lead 3.Dayton, OH - Histo Tech 4.Pittsburgh, PA - Histo Tech 5.Richmond, VA - Histo Tech If you are interested in these jobs, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well. I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 234 TOLLFREE: 800-466-9919 ext 234 EMAIL: pam@ategra.com WEBSITE: --------------------------------------------------------- From pjfnefro <@t> duke.edu Tue Dec 2 15:00:48 2003 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Citrate Buffer pH Message-ID: <3FCCFD80.9070805@duke.edu> I have a procedure for deparaffinizing (their word, not mine) tissue sections in order to do immunostaining using the Vector MOM kit. It calls for heating the section (briefly) in a microwave in 0.1M Citrate Buffer. The procedure does not specify the pH of the buffer, just to make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound familiar to anyone out there? -- -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 From pruegg <@t> colobio.com Tue Dec 2 15:22:14 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Re: Immuno & Insitu Workshops In-Reply-To: Message-ID: Mark and everyone, I would like to invite you to attend the upcoming meeting in Colorado at the Stanley Hotel (The Shinning was filmed here) in Estes Park, April 23-24, 2004. We have lots of IHC on tap. Go to the Colorado web site for complete information at: www.coloradohisto.org Patsy Ruegg -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of mark.lewis@thermo.com Sent: Tuesday, December 02, 2003 10:27 AM Cc: Histonet (E-mail); histonet-admin@lists.utsouthwestern.edu Subject: [Histonet] Re: Immuno & Insitu Workshops Hello everyone, I'd like to know if there are any upcoming workshops for Immuno Histochemistry, In Situ or FISH. Particularly interested in any that deal with trouble shooting, but am still interested in any related workshops. Thanks ! Mark Mark Lewis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Tue Dec 2 15:42:22 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] fixing bone marrow for IHC In-Reply-To: Message-ID: Why would you do antigen retrieval on tissue not formalin fixed? Patsy Ruegg -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of MARY T HODGES Sent: Thursday, November 27, 2003 9:30 AM To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu; scoop@mail.nih.gov Subject: Re: [Histonet] fixing bone marrow for IHC Good Morning, I have used Methanol and acetone for smears and had great results. 15 mins... let air dry ...15 mins and let air dry. They do well even through antigen retrival. good luck Tere Hodges St Mary's Hospital Tucson,Az. >From: "Richard Cartun" >To: , >Subject: Re: [Histonet] fixing bone marrow for IHC >Date: Tue, 25 Nov 2003 16:56:06 -0500 > >We use formalin. I hate to see anyone use B5 because of environmental >concerns with mercury. > >Richard Cartun >Director, Immunopathology >Hartford Hospital > > >>> Sharon Cooperman 11/25/03 04:16PM >>> >Does anyone have an opinion on the best way to fix bone marrow smears >for immunohistochemistry? So far I've heard methanol, acetone, B-5 >and formalin. The antibodies I plan to use all work on formalin >fixed paraffin embedded tissue. I'd appreciate any advice or >opinions. > >Thanks, >Sharon > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ online games and music with a high-speed Internet connection! Prices start at less than $1 a day average. https://broadband.msn.com (Prices may vary by service area.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Tue Dec 2 15:41:14 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:17 2005 Subject: network down...RE: [Histonet] Histotechnology trivia Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CDBA@usca0082k03.rallansci.apogent.com> Diane wrote:<< ...hospital computer system has been down for 2 days...>> Hmmm... I was at an IT conference earlier this year at which a speaker stated categorically "...network computer systems don't go down anymore." Wonder what planet he was from!? Tim -----Original Message----- From: Gladney, Diane C Ms MACH [mailto:Diane.Gladney@se.amedd.army.mil] Sent: Tuesday, December 02, 2003 12:37 PM To: 'Ford Royer'; Histo Net Subject: RE: [Histonet] Histotechnology trivia Thanks, I needed that laugh....hospital computer system has been down for 2 days and has just now been restored to operation. Lots of info to enter into LIS now.... I really needed this light hearted commentary. Swamped With Paper Work, Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: Ford Royer [mailto:froyer@bitstream.net] Sent: Tuesday, December 02, 2003 3:28 PM To: Histo Net Subject: Re: [Histonet] Histotechnology trivia MTitford@aol.com wrote: >I got a card today telling me Thermo Shandon is now Thermo Electron, the latest step in companies gobbleing each other up. > >Only a few years ago there was the Shandon and Lipshaw companies. Shandon took over Lipshaw, who were in turn taken over by Thermo electron. > >And then years ago Spencer Microtomes were taken over by American Optical, who were taken over by Reichert, and then Leica. Is that right? Will there be only one or two histology equipment companies soon? > >Mike Titford >USA Pathology >Mobile AL USA > I hear you Mike... it's almost impossible to keep up with all the mergers, and not just in Histology. Here's a funny that relates to the topic: Investment tips for 2004.... for all of you with any money left In the wake of the Exxon/Mobile deal and the AOL/TimeWarner implode be aware of the next expected mergers so that you can get in on the ground floor and make some BIG bucks. Watch for these consolidations in 2004: 1. Hale Business Systems, Mary Kay Cosmetics, Fuller Brush, and W. R.Grace Co. will merge and become: Hale, Mary, Fuller, Grace. 2. Polygram Records, Warner Bros., and Zesta Crackers join forces and become: Poly, Warner Cracker. 3. 3M will merge with Goodyear and issue forth as: MMMGood. 4. Zippo Manufacturing, Audi Motors, Dofasco, and Dakota Mining will merge and become: ZipAudiDoDa. 5. FedEx is expected to join its major competitor, UPS, and become: FedUP. 6. Fairchild Electronics and Honeywell Computers will become: Fairwell Honeychild. 7. Grey Poupon and Docker Pants are expected to become: Poupon Pants. 8. Knotts Berry Farm and the National Organization of Women will become: Knott NOW! That's all for now.....invest wisely! Ford Royer, MT(ASCP) Analytical Instruments Minneapolis, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Dec 2 15:43:42 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Spam faxes and emails from sales reps Message-ID: I was just handed a fax from a sales rep ADVERTISING that December is the last month I can get a FREE pressure cooker if I order a jillion dollars worth of AR solution. I like SPAM faxes just about as much as I like SPAM email and telemarketing calls during dinner. I know my company doesn't appreciate this abuse of their fax system, not to mention the waste of paper and jamming our fax line. I won't rag on the company by name, because I actually like the vendor -BUT I HATE THIS METHOD OF ADVERTISING. I feel like the rep is shoving products down my throat. I know the rep got my fax number off this listserver, so I've taken my number off my automatic signature. Does anyone else have as much disdain for this intrusive method of advertising?? Bah, humbug. Jackie O' Whereabouts, unknown Don't call, don't fax From AnthonyH <@t> chw.edu.au Tue Dec 2 15:46:46 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] isolator/pap pens Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0D8@simba.kids> Loralee, Try Zymed or DAKO Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Gehan, Loralee [mailto:Loralee_Gehan@URMC.Rochester.edu] Sent: Wednesday, 3 December 2003 1:38 AM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] isolator/pap pens Does anyone have a good source for isolator or pap pens? Besides biocare. I have had a stream on bad pens and they are not too cheap. Thanks in advance. Loralee Gehan University of Rochester Orthopaedics Research Department _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Jackie.O'Connor <@t> abbott.com Tue Dec 2 15:50:04 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Citrate Buffer pH Message-ID: I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company. It works great - saves a bunch o' steps. You might try contacting them for the specifics. I don't think about the nature of these things if they work - I just follow along blindly . . . . . . . . .sometimes. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics Pat Flannery Sent by: histonet-admin@lists.utsouthwestern.edu 12/02/2003 03:00 PM To: Histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Citrate Buffer pH I have a procedure for deparaffinizing (their word, not mine) tissue sections in order to do immunostaining using the Vector MOM kit. It calls for heating the section (briefly) in a microwave in 0.1M Citrate Buffer. The procedure does not specify the pH of the buffer, just to make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound familiar to anyone out there? -- -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031202/b1b9ce31/attachment.htm From pruegg <@t> colobio.com Tue Dec 2 16:03:15 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Seep macrophage marker In-Reply-To: <5.1.0.14.0.20031127162039.00a34640@mail.jcu.edu.au> Message-ID: Laurie, I have done CD68 staining on human, rat, mouse, monkey and rabbit, but have not tried it on sheep yet. My cross-reactivity chart lists CD68 as untried on sheep as well. One thing that I have found is that the clone PG-M1 is much more specific to just macrophages and monocytes and not myeloid cells and a variety of tissues as is clone KP1. I would not hesitate to try PG-M1 in sheep tissue. I will contact my local sheep investigators and see if they have done this. We have a facility here at UCHSC that just studies sheep. Sorry I couldn't be more helpful, keep bugging me to look into this in case it slips my mind. Best regards, Patsy Ruegg -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Laurie Reilly Sent: Wednesday, November 26, 2003 11:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seep macrophage marker Dear All, Does anyone have experience in immuno staining of macrophages in sheep tissues? Any help would be appreciated. Regards and thanks, Laurie. Mr.Laurie Reilly Ph 07 4781 4468 Physiology & Pharmacology Fax 07 4779 1526 Aust.Inst.of Tropical Vet.& Animal Sc. James Cook University Townsville Qld. 4811 laurie.reilly@jcu.edu.au Australia. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MinHan.Tan <@t> vai.org Tue Dec 2 17:05:09 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Citrate Buffer pH Message-ID: <74D0F0AB07F2E647A02D839ED79520F97B9BF9@VAIEXCH02.vai.org> AFAIK, the usual pH is 6.0 Min-han Tan -----Original Message----- From: Pat Flannery [mailto:pjfnefro@duke.edu] Sent: Tuesday, December 02, 2003 4:01 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Citrate Buffer pH I have a procedure for deparaffinizing (their word, not mine) tissue sections in order to do immunostaining using the Vector MOM kit. It calls for heating the section (briefly) in a microwave in 0.1M Citrate Buffer. The procedure does not specify the pH of the buffer, just to make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound familiar to anyone out there? -- -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. From edonato <@t> fhcrc.org Tue Dec 2 17:20:33 2003 From: edonato <@t> fhcrc.org (Donato, Elizabeth M) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Does anyone have a used embedding center? Message-ID: <07470B0DBFBDD511BD770002B330A37DC39FAC@curly.fhcrc.org> Hello, We are looking to purchase a used embedding center and thought that maybe someone out there in the land of histology might have one that they aren't using???? If you happen to have an older model (with or without the cooling unit) and you are interested in selling it, please contact me. Thanks in advance for your help! Kind regards, Liz Donato Fred Hutchinson Cancer Research Center Seattle WA 98109-1024 206.667.4501 ph 206.667.5815 fax From victoria.hahn-stromberg <@t> orebroll.se Wed Dec 3 09:31:26 2003 From: victoria.hahn-stromberg <@t> orebroll.se (victoria.hahn-stromberg@orebroll.se) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] immunohistochemistry c-myc, max protein Message-ID: <0875915A604AF444AAB9AB43FD6DE8C72AFF6E@storm.orebroll.se> Hi everyone! Has anybody tried to stain paraffin embedded sections, 4um, for the maxprotein of C-myc? Is it possible? Victoria Hahn-Str?mberg MSc, PhD student Departement of Pathology ?rebro University hospital S-701 85 ?rebro, Sweden email: victoria.hahn-stromberg@orebroll.se From ree3 <@t> leicester.ac.uk Wed Dec 3 10:29:37 2003 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Fra-1 Message-ID: I am after an antibody to Fra-1, fos related antigen, so far I have only been able to find antibodies to Fra-2. Thanks Richard Edwards MRC TOXICOLOGY UNIT LEICESTER......U.K..... From jcox90 <@t> yahoo.com Wed Dec 3 10:58:05 2003 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Stain for H.Pylori Message-ID: <20031203165805.14533.qmail@web40905.mail.yahoo.com> Hello everyone, Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it? Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab --------------------------------- Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/660d307c/attachment.htm From cycling <@t> another.com Wed Dec 3 11:31:23 2003 From: cycling <@t> another.com (cycling@another.com) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Trichrome staining Message-ID: <7309756.1070472684012.JavaMail.root@172.16.100.50> Relationship between axial periodicity and staining of collagen by the Masson trichrome procedure M.H.Flint & M.J.Merrilees Histochemical Journal Vol 9 No.1 Jan 1977 I think that there were other papers in that era, probably lots but this one only took a couple of seconds to find on the shelf. Dave Ed Manchester UK -- Personalised email by http://another.com From myrtelina <@t> hotmail.com Wed Dec 3 11:52:27 2003 From: myrtelina <@t> hotmail.com (Myrtelina Fernandez) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Mountig medium Message-ID: How do you prevent mountig medium from "runnig" after coverslip and clean the slide. Frequently, even when a very small amount of the product is used ,the slides get stick to the tray due to the mounting medium on the edge. At this moment the lab. staff double check the slides before submitting them to screening, but sometimes, due to time frame or distraction, they can forget to do it. Some days are worst than others. We are using Eukitt mounting medium, which is"fast drying".and I don't know if submitting the slides to heat righ after coverslip would alter its properties.Would it work? Is this a common problem or is just that we have to be more careful? Iwill appreciate some ideas. Thanks. M. Fernández, Laboratory supervisor _________________________________________________________________ Protect your PC - get McAfee.com VirusScan Online http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From pruegg <@t> colobio.com Wed Dec 3 12:18:36 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Citrate Buffer pH In-Reply-To: Message-ID: Has anybody tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer in place of the pressure cooker? PC is too harsh for some of my stuff but I like using these retrieval/deparaffination reagents. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com Sent: Tuesday, December 02, 2003 2:50 PM To: Pat Flannery Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu Subject: Re: [Histonet] Citrate Buffer pH I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company. It works great - saves a bunch o' steps. You might try contacting them for the specifics. I don't think about the nature of these things if they work - I just follow along blindly . . . . . . . . .sometimes. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics Pat Flannery Sent by: histonet-admin@lists.utsouthwestern.edu 12/02/2003 03:00 PM To: Histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Citrate Buffer pH I have a procedure for deparaffinizing (their word, not mine) tissue sections in order to do immunostaining using the Vector MOM kit. It calls for heating the section (briefly) in a microwave in 0.1M Citrate Buffer. The procedure does not specify the pH of the buffer, just to make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound familiar to anyone out there? -- -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/33f03850/attachment.htm From haldana <@t> unimoron.edu.ar Wed Dec 3 12:20:16 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Stain for H.Pylori References: <20031203165805.14533.qmail@web40905.mail.yahoo.com> Message-ID: <003701c3b9ca$1b7e4be0$7504a8c0@um.edu> MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER FIXATION: Neutral Formolin 10% SECCIONS: 4-5 ?m SOLUTIONS: ACETIC WATER Acetic acid............... 0,01 ml Destilated water............... 1000,00 ml Working solution GIEMSA Giemsa.............. 10,0 ml Acetic water.............. 50,0 ml PROCEDDURE 1. Take slides to water destiled 2. Giemsa working solution 1 h 3. Deshidratation in 3 changes of alcohol 100 4. xilol, syntetic mounting medium RESULTADOS H. pylorii.............. intense blue Nuclei.............. blue Cytoplasm.............. Rose REFERENCE Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984 Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: Jill Cox To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 03, 2003 1:58 PM Subject: [Histonet] Stain for H.Pylori Hello everyone, Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it? Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab ------------------------------------------------------------------------------ Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/dcf39021/attachment.htm From trapani <@t> spot.colorado.edu Wed Dec 3 12:38:45 2003 From: trapani <@t> spot.colorado.edu (Josh Trapani) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] cryosectioning adult fish teeth? Message-ID: Dear All, I have been attempting to cryosection pharyngeal teeth (and associated structures) of adult fish for immunohistochemistry, with very limited success. I have been working mostly with zebrafish but also with some cichlids; adult body lengths are < 5 cm. The problem is that even when the section as a whole is good, the tissue within it contracts, folds, and just doesn't hold together. The protocol I am currently using is as follows: -Immediately after death, branchial arches are dissected out and fixed overnight in 4% paraformaldehyde in PBS at 4 C. -Fixed material is then transferred to PolyNoCal (Polysciences); decalcification takes < 24 h at 4 C. -Material is then transferred to 30% sucrose solution and left overnight or until the specimen sinks...this also at 4 C. -Finally, material is embedded in Tissue-Tek OCT, frozen carefully in liquid nitrogen, mounted and cut at -20 C. Optimally, I would like sections of 15 microns thickness. Some variations I have tried that don't improve things much include: sectioning whole heads instead of dissecting out branchial arches, longer fixation times, decalcification in 4% EDTA (takes a lot longer), freezing of specimens in the cryostat just prior to sectioning rather than with liquid nitrogen beforehand, and cutting sections up to 30 microns thick (thicker sections do generally hold together better, but it's still not too good). Searches of the literature have not helped me come up with a better protocol. Something tells me if I had more histological experience, the solution would be obvious, but I may be wrong. My best guess right now is some incompatibility between the tissue and the freezing medium, so I'm looking into alternative freezing media. If anyone has any suggestions or knows of a protocol that works (for cryosectioning mineralized tissues in adult fish, or any lower vertebrates really), I would be very appreciative. My apologies if this question has come up before (though somehow I doubt it!). Josh Trapani Postdoctoral Research Associate Department of Ecology and Evolutionary Biology University of Colorado Boulder, CO 80309-0334 From fcs <@t> uevora.pt Wed Dec 3 13:02:35 2003 From: fcs <@t> uevora.pt (Fernando Capela e Silva) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Plant histology Message-ID: <008501c3b9d0$04fafe80$aad988c1@uevora.pt> I would like receive information, a protocol if possible, about histology of plant tissues, particularly the little branch, with semi lignification, of olive tree. With compliments * * * * * * * * * * * * * * * * * * * * * * * * * Fernando Capela e Silva Laborat?rio de Morfologia Funcional UBC - Unidade de Biologia da Conserva??o Departamento de Biologia - Universidade de ?vora Apartado 94 7002-554 ?vora PORTUGAL Phone: +351-266 760 881 Fax: +351-266 760 914 Email: fcs@uevora.pt http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/71cc3689/attachment.htm From info <@t> instrumedics.com Wed Dec 3 13:24:12 2003 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] CureMount Mounting Medium Message-ID: <007201c3b9d3$08578fa0$6401a8c0@instrumedics1> Instrumedics' CureMount Mounting Medium is designed for use with dehydrated and cleared sections. The cover slipped slide is placed under a UV light for 20 seconds which converts the "liquid" into a "solid" with a refractive index of 1.55 which matches the refractive index of the section. When viewed in the microscope the stained elements stand out and the background becomes virtually invisible. No more sticking to the slide tray or to a neighboring slide. The slide is "dry" and can be archived immediately. Bernice schiller@instrumedics.com From pruegg <@t> colobio.com Wed Dec 3 13:28:43 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] cryosectioning adult fish teeth? In-Reply-To: Message-ID: Josh, It is difficult to cut teeth no matter what, but I would say your best bet would be to use the tape transfer sectioning aide from Instrumedics. I have cut whole calcified rat femurs using this technique and got decent sections. There can be a problem with doing IHC on these sections though. You use a polymer coated slide to attach the tape section to and the coating can interfere or cause background in IHC. I am at Fitzsimmons in Aurora and would be happy to let you come over to my lab and try to cut your sections in my cryostat using my tape system, or you could send me some frozen samples and I could try to cut them for you. Patsy Ruegg 720-859-4060 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Josh Trapani Sent: Wednesday, December 03, 2003 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryosectioning adult fish teeth? Dear All, I have been attempting to cryosection pharyngeal teeth (and associated structures) of adult fish for immunohistochemistry, with very limited success. I have been working mostly with zebrafish but also with some cichlids; adult body lengths are < 5 cm. The problem is that even when the section as a whole is good, the tissue within it contracts, folds, and just doesn't hold together. The protocol I am currently using is as follows: -Immediately after death, branchial arches are dissected out and fixed overnight in 4% paraformaldehyde in PBS at 4 C. -Fixed material is then transferred to PolyNoCal (Polysciences); decalcification takes < 24 h at 4 C. -Material is then transferred to 30% sucrose solution and left overnight or until the specimen sinks...this also at 4 C. -Finally, material is embedded in Tissue-Tek OCT, frozen carefully in liquid nitrogen, mounted and cut at -20 C. Optimally, I would like sections of 15 microns thickness. Some variations I have tried that don't improve things much include: sectioning whole heads instead of dissecting out branchial arches, longer fixation times, decalcification in 4% EDTA (takes a lot longer), freezing of specimens in the cryostat just prior to sectioning rather than with liquid nitrogen beforehand, and cutting sections up to 30 microns thick (thicker sections do generally hold together better, but it's still not too good). Searches of the literature have not helped me come up with a better protocol. Something tells me if I had more histological experience, the solution would be obvious, but I may be wrong. My best guess right now is some incompatibility between the tissue and the freezing medium, so I'm looking into alternative freezing media. If anyone has any suggestions or knows of a protocol that works (for cryosectioning mineralized tissues in adult fish, or any lower vertebrates really), I would be very appreciative. My apologies if this question has come up before (though somehow I doubt it!). Josh Trapani Postdoctoral Research Associate Department of Ecology and Evolutionary Biology University of Colorado Boulder, CO 80309-0334 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGordon <@t> cellmarque.com Wed Dec 3 13:54:46 2003 From: JGordon <@t> cellmarque.com (Jeff Gordon) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Citrate Buffer pH Message-ID: Patsy, we have a steamer protocol for using our all in one deparaffinization and unmasking solutions Declere and Trilogy (US patent # 6,649,386) in a steamer. We don't endorse it as often because we were looking to standardization and there were some antibodies that needed the pressure cooker heat to work optimally (such as ALK-1, Cyclin D1, CD10, and TTF-1) and so we chose to just continue to endorse using the pressure cooker. The steamer one step method, however, works quite well, only it takes a bit longer (60 total minutes of steam time) than in the pressure cooker. It is located on page 54 of the current Cell Marque reference guide. Jeff Gordon Cell Marque Corp. -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, December 03, 2003 12:19 PM To: Jackie.O'Connor@abbott.com; Pat Flannery Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu Subject: RE: [Histonet] Citrate Buffer pH Has anybody tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer in place of the pressure cooker? PC is too harsh for some of my stuff but I like using these retrieval/deparaffination reagents. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com Sent: Tuesday, December 02, 2003 2:50 PM To: Pat Flannery Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu Subject: Re: [Histonet] Citrate Buffer pH I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company. It works great - saves a bunch o' steps. You might try contacting them for the specifics. I don't think about the nature of these things if they work - I just follow along blindly . . . . . . . . .sometimes. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics Pat Flannery Sent by: histonet-admin@lists.utsouthwestern.edu 12/02/2003 03:00 PM To: Histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Citrate Buffer pH I have a procedure for deparaffinizing (their word, not mine) tissue sections in order to do immunostaining using the Vector MOM kit. It calls for heating the section (briefly) in a microwave in 0.1M Citrate Buffer. The procedure does not specify the pH of the buffer, just to make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound familiar to anyone out there? -- -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/1b326996/attachment.htm From subratab <@t> bdonline.com Wed Dec 3 14:25:19 2003 From: subratab <@t> bdonline.com (Subratab) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Perfused versus non-perfused tissue for IHC Message-ID: <200312032026.hB3KQ3Du020218@korotoa.bdonline.com> Dear All For IHC some researchers use perfused tissue section. Others use non-perfused tissue for the study of the same antigen. My questions are: 1. What are the advantages of using perfused tissue over non-perfused tissue for IHC staining? 2. For what type of antigen study tissue perfusion is essential? 3. What type of differences may I encounter between perfused and non-perfused tissue stained for the same antigen? Thank you in advance for answer and clarification. Please let me know any link (like website or article) where I can get detail discussion on this issue Subrata Biswas Nephrology Div of Internal Med UNICAMP, SP, Brazil. From Ronnie_Houston <@t> bshsi.com Wed Dec 3 14:53:23 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Citrate Buffer pH Message-ID: <530361BF03351B4CAE5270A05D3037B5FE057E@bsrexms01.BSHSIR.COM> Patsy, We routinely use Reveal and Borg, both from Biocare Medical, with great success in a B&D steamer, 20 minutes, followed by 2 rinses in hot buffer, and 20 minute cool down. Very rarely use pressure cooker, and don't use micrwave at all. Ronnie Ronnie Houston Regional Histology Operations Manager Bon Secours HealthPartners Laboratories 5801 Bremo Road Richmond, VA 23226 (804) 287 7972 ronnie_houston@bshsi.com -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, December 03, 2003 1:19 PM To: Jackie.O'Connor@abbott.com; Pat Flannery Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu Subject: RE: [Histonet] Citrate Buffer pH Has anybody tried the AR reagents that deparaffinzed such as BioCare Decloaker in a steamer in place of the pressure cooker? PC is too harsh for some of my stuff but I like using these retrieval/deparaffination reagents. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jackie.O'Connor@abbott.com Sent: Tuesday, December 02, 2003 2:50 PM To: Pat Flannery Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu Subject: Re: [Histonet] Citrate Buffer pH I've been using a product from Biocare, a Universal Decloaker (6.0 citrate buffer) , which deparaffinizes during HIER in their pressure cooker (Decloaking Chamber) - they are a very Trekkie oriented company. It works great - saves a bunch o' steps. You might try contacting them for the specifics. I don't think about the nature of these things if they work - I just follow along blindly . . . . . . . . .sometimes. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics Pat Flannery Sent by: histonet-admin@lists.utsouthwestern.edu 12/02/2003 03:00 PM To: Histonet@lists.utsouthwestern.edu cc: Subject: [Histonet] Citrate Buffer pH I have a procedure for deparaffinizing (their word, not mine) tissue sections in order to do immunostaining using the Vector MOM kit. It calls for heating the section (briefly) in a microwave in 0.1M Citrate Buffer. The procedure does not specify the pH of the buffer, just to make it by mixing 0.1M NaCitrate and 0.1M citric acid. Does this sound familiar to anyone out there? -- -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/b2b4b60e/attachment.htm From edonato <@t> fhcrc.org Wed Dec 3 15:04:53 2003 From: edonato <@t> fhcrc.org (Donato, Elizabeth M) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] RE: Does anyone have a used embedding center? Message-ID: <07470B0DBFBDD511BD770002B330A37DC39FB8@curly.fhcrc.org> A great big thanks to all that responded to my request via phone and email!! Thanks to your help I think that we will be able to find an embedding center that meets our needs within our budget. Liz From: "Donato, Elizabeth M" To: histonet@lists.utsouthwestern.edu Date: Tue, 2 Dec 2003 15:20:33 -0800 Subject: [Histonet] Does anyone have a used embedding center? Hello, We are looking to purchase a used embedding center and thought that maybe someone out there in the land of histology might have one that they aren't using???? If you happen to have an older model (with or without the cooling unit) and you are interested in selling it, please contact me. Thanks in advance for your help! Kind regards, Liz Donato Fred Hutchinson Cancer Research Center Seattle WA 98109-1024 206.667.4501 ph 206.667.5815 fax From frank <@t> purdue.edu Wed Dec 3 15:46:24 2003 From: frank <@t> purdue.edu (Frank Rosenthal) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] sectioning old GMA blocks Message-ID: Dear histonetters: I have a 5030 Hacker rotary microtome (bench model) and I am trying to understand the mechanics of how the tissue block advances toward the knife. (I don't know if the same principles applies to all rotary microtomes.) Apparently different portions of a constant radius arc are used but I don't quite understand it. Has anyone seen a good explanation of this? Thanks. Frank ***************************************** Frank S. Rosenthal, Ph.D. Associate Professor Purdue University School of Health Sciences 1338 Civil Engineering Building West Lafayette, IN 47907 USA tel: 765-494-0812, fax: 765-496-1377, e-mail: frank@purdue.edu ***************************************** From nick.kirk3 <@t> btopenworld.com Wed Dec 3 15:53:25 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Stain for H. Pylori In-Reply-To: <20031203165805.14533.qmail@web40905.mail.yahoo.com> Message-ID: We routinely do the Alcian Yellow/Tol blue for H.pylori and the Pathologists love it because of the better colour contrast of the blue bacteria on a yellow background, compared to say a Giemsa for instance (blue on blue). The only problem at the moment is that production of Alcian Yellow has stopped apparently, so it's difficult if not impossible to order new stock of the stain. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Jill Cox Sent: 03 December 2003 16:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stain for H.Pylori Hello everyone, Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it? Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab ---------------------------------------------------------------------------- -- Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031203/1e8cec0c/attachment.htm From Inga.Hansson <@t> neuro.uu.se Thu Dec 4 02:45:14 2003 From: Inga.Hansson <@t> neuro.uu.se (Inga Hansson) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] microglia Message-ID: Hi all! Can anyone suggest a good antibody for microglia that works on frozen and FFPE-tissue in mice? Thanks in advance! Inga -- Inga Hansson Dept. neuroscience, div. neurobiology PO Box 587, BMC Husargatan 3 S-751 23 Uppsala SWEDEN Phone: +46(18) 471 4384 Fax: +46(18)559017 From p.munday <@t> inpharmatica.co.uk Thu Dec 4 05:11:29 2003 From: p.munday <@t> inpharmatica.co.uk (Peter Munday) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] In-situ hybridization help please Message-ID: <000601c3ba57$5dd6b8a0$267ba8c0@windows.inpharmatica.co.uk> Hello Histonetters, I am currently doing in-situ hybridization on paraffin embedded tissue array sections. Very often I get a signal in the nucleus, which I think is a little odd as I would expect to see it in the cytoplasm. Has anybody else encounted this? If so, is it real? If it isn't real, what can be done about it? Thanks, Peter Munday Inpharmatica London From Wanda.Smith <@t> HCAhealthcare.com Thu Dec 4 07:51:15 2003 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Sep 16 15:22:17 2005 Subject: [Histonet] Pathologist's Assistant Assistant? Message-ID: <8784A3EE34199644AF60DBE517F5B0A604A415AD@louex04.lou.medcity.net> Dear Histonetters, I do not want to open a HUGE can of worms regarding PA's, but I have a question that I need some input on... At facilities that use PA's, is there an Assistant to the Assistant at the grossing table??? In other words, Is there a Lab Assistant or Histotech always available when the PA is cutting? The reason I ask is, we are staffed very thinly, and late in the afternoon, things get busy with the phones, accessioning specimens, late frozen sections, etc, etc and I could use the Lab assistant to do other things rather than stand w/ the PA and change blades... Input on this is appreciated and let me say, I do not want to open the "Let's bash the PA's" can of worms!!! Thanks, Wanda > Wanda G. Smith, HTL/HT(ASCP) > Pathology Supervisor > Trident Medical Center Laboratory Services > *9330 Medical Plaza Drive, Charleston, SC 29406 > *843-797-4586 *fax 843-797-4296 > *wanda.smith@hcahealthcare.com > > > From cgfields <@t> lexhealth.org Thu Dec 4 08:13:11 2003 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Pathologist's Assistant Assistant? Message-ID: Hi Wanda, We have support techs that assist w/gross, make cassettes, close cassettes, put the cassettes in the formalin bath under the correct doctor, keep up with the grossing log, pickup specimens, accession, and in general keep things moving. I guess it depends on what their responsibilities are if you need them or not. We have two or three PA's grossing at a time and sometimes a Pathologist. We very much need our support techs. Carole Fields Lex.Med.Ctn. W.Columbia, SC > -----Original Message----- > From: Smith Wanda [SMTP:Wanda.Smith@HCAhealthcare.com] > Sent: Thursday, December 04, 2003 8:51 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Pathologist's Assistant Assistant? > > Dear Histonetters, > I do not want to open a HUGE can of worms regarding PA's, but I have a > question that I need some input on... > At facilities that use PA's, is there an Assistant to the Assistant at the > grossing table??? In other words, Is there a Lab Assistant or Histotech > always available when the PA is cutting? > The reason I ask is, we are staffed very thinly, and late in the > afternoon, > things get busy with the phones, accessioning specimens, late frozen > sections, etc, etc and I could use the Lab assistant to do other things > rather than stand w/ the PA and change blades... > Input on this is appreciated and let me say, I do not want to open the > "Let's bash the PA's" can of worms!!! > Thanks, > Wanda > > > Wanda G. Smith, HTL/HT(ASCP) > > Pathology Supervisor > > Trident Medical Center Laboratory Services > > *9330 Medical Plaza Drive, Charleston, SC 29406 > > *843-797-4586 *fax 843-797-4296 > > *wanda.smith@hcahealthcare.com > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031204/b35eb11c/attachment.htm From Tony.Fearn <@t> cd.burnleyhc-tr.nwest.nhs.uk Thu Dec 4 08:30:43 2003 From: Tony.Fearn <@t> cd.burnleyhc-tr.nwest.nhs.uk (Fearn Tony) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours Message-ID: <8C3DE9069A06D611B8950002A550C15943BCFF@BHC_MAIL02> We're trying to differentiate between a carcinoid tumour and other neuroendocrine tumours. Grimelius, chromogranin & synaptophysin are positive and we need to be more specific as to the type of tumour, with very little tissue left in the block! I've been told staining for argentaffin granules would be best, but does anyone know which method is the best to go for? Thanks, in advance, for your help. Lesley From siksik03 <@t> comcast.net Thu Dec 4 08:56:47 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Citrate Buffer pH In-Reply-To: <530361BF03351B4CAE5270A05D3037B5FE057E@bsrexms01.BSHSIR.COM> References: <530361BF03351B4CAE5270A05D3037B5FE057E@bsrexms01.BSHSIR.COM> Message-ID: Hi HistoNetters As Dr. Shi points out in his book and articles about antigen retrieval, there are three parameters involved in achieving consistent results: 1) temperature 2) time 3) pH How you get there (steamer, water bath, microwave, pressure cooker) doesn't matter, so long as you can successfully monitor these three variables very accurately. As Jeff Gordon correctly points out, some antigens need temperatures higher than 98?C for optimal retrieval, however. For this reason, pressure cookers, whether by themselves, or inside of a microwave have been used to reach temperatures as high as 120?C as needed. Using a combination microwave/pressure method, I have been able to use citrate buffer at pH 6.0 to retrieve most antigens at 110?C in 3-5 minutes. best regards, Steven Slap At 3:53 PM -0500 12/3/03, Houston, Ronnie wrote: >Patsy, > >We routinely use Reveal and Borg, both from >Biocare Medical, with great success in a B&D >steamer, 20 minutes, followed by 2 rinses in hot >buffer, and 20 minute cool down. Very rarely use >pressure cooker, and don't use micrwave at all. > >Ronnie > >Ronnie Houston >Regional Histology Operations Manager >Bon Secours HealthPartners Laboratories >5801 Bremo Road >Richmond, VA 23226 >(804) 287 7972 >ronnie_houston@bshsi.com > > >-----Original Message----- >From: Patsy Ruegg [mailto:pruegg@colobio.com] >Sent: Wednesday, December 03, 2003 1:19 PM >To: Jackie.O'Connor@abbott.com; Pat Flannery >Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu >Subject: RE: [Histonet] Citrate Buffer pH > >Has anybody tried the AR reagents that >deparaffinzed such as BioCare Decloaker in a >steamer in place of the pressure cooker? PC is >too harsh for some of my stuff but I like using >these retrieval/deparaffination reagents. >Patsy > >-----Original Message----- >From: histonet-admin@lists.utsouthwestern.edu >[mailto:histonet-admin@lists.utsouthwestern.edu]On >Behalf Of Jackie.O'Connor@abbott.com >Sent: Tuesday, December 02, 2003 2:50 PM >To: Pat Flannery >Cc: Histonet@lists.utsouthwestern.edu; histonet-admin@lists.utsouthwestern.edu >Subject: Re: [Histonet] Citrate Buffer pH > > >I've been using a product from Biocare, a >Universal Decloaker (6.0 citrate buffer) , which >deparaffinizes during HIER in their pressure >cooker (Decloaking Chamber) - they are a very >Trekkie oriented company. It works great - >saves a bunch o' steps. You might try >contacting them for the specifics. I don't >think about the nature of these things if they >work - I just follow along blindly . . . . . . . >. .sometimes. > >Jacqueline M. O'Connor HT(ASCP) >Abbott Laboratories >Global Pharmaceutical Research and Development >Discovery Chemotheraputics -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031204/c830d5e3/attachment.htm From algranth <@t> u.arizona.edu Thu Dec 4 09:21:00 2003 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Plant histology In-Reply-To: <008501c3b9d0$04fafe80$aad988c1@uevora.pt> Message-ID: <4.3.2.7.2.20031204081449.00cb42c0@algranth.inbox.email.arizona.edu> Fernando, I've started doing a bit of plant tissue so I just recently got the book, "Plant Microtechnique and Microscopy" by Steven Ruzin. A fellow histonetter passed along Steve's email when I was searching for processing techniques for seeds and Steve was very helpful. Several of the techniques he suggested came from his book. Amazon.com has the book, new for $51.00(US) Andi Grantham At 07:02 PM 12/3/2003 +0000, you wrote: >I would like receive information, a protocol if possible, about histology >of plant tissues, particularly the little branch, with semi lignification, >of olive tree. > > >With compliments >* * * * * * * * * * * * * * * * * * * * * * * * * >Fernando Capela e Silva >Laborat?rio de Morfologia Funcional >UBC - Unidade de Biologia da Conserva??o >Departamento de Biologia - Universidade de ?vora >Apartado 94 >7002-554 ?vora >PORTUGAL > >Phone: +351-266 760 881 >Fax: +351-266 760 914 >Email: fcs@uevora.pt > >http://evunix.uevora.pt/~fcs/FernandoCapelaSilva.htm ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Tony.Fearn <@t> cd.burnleyhc-tr.nwest.nhs.uk Thu Dec 4 09:46:14 2003 From: Tony.Fearn <@t> cd.burnleyhc-tr.nwest.nhs.uk (Fearn Tony) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] CD56 Message-ID: <8C3DE9069A06D611B8950002A550C15943BD00@BHC_MAIL02> We've just got Novocastra's CD56. What pre-treatment does everyone use? We've found that pressure cooking using Vector's antigen retrieval solution is best, but it's still not overly strong for neuroendocrine cells, with a 1:50 dilution. Lesley Smith From info <@t> instrumedics.com Thu Dec 4 09:17:53 2003 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #157 - 17 msgs References: <20031204143500.21467.65335.Mailman@swlx167.swmed.edu> Message-ID: <004101c3ba81$aeb2b240$6401a8c0@instrumedics1> Josh, I think that the CryoJane Tape-Transfer might solve your problem as Patsy suggests. Also if you use the CureMount Mounting Medium you should be able to get good results with the immunostaining since the background is made virtually invisible! Bernice From mcauliff <@t> umdnj.edu Thu Dec 4 10:11:46 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] mouse microglia In-Reply-To: References: Message-ID: <3FCF5CC2.50006@umdnj.edu> Hi Inga: "Mac1" or "F4/80" will stain microglia in frozen sections of mouse brain. Fix by perfusion with buffered paraformaldehyde, remove the brain and fix for another 2 hours, no longer (at least for Mac1). Then rinse multiple times in buffer, cyroprotect with sucrose, cut 20 micron frozen sections, store in PBS, stain free-floating, etc. I have stored sections in PBS in the refrigerator for several weeks with no loss of antigenicity. Sections air-dired on slides lose microglial antigenicity very quickly (2 days). Antibodies for rat microglia will not work on mouse. Clint Lincoln has stained mouse microglia in FFPE sections, he used a enzyme digestion protocol (pronase?) to unmask the antigenic sites. I don't know if I can find his recipe. See: Lawson et al., Neuroscience 39:151-170, 1990 and 48:405-415, 1992 and Perry et al., Neuroscience 15:315-326, 1985. Geoff Inga Hansson wrote: > Hi all! > > Can anyone suggest a good antibody for microglia that works on frozen > and FFPE-tissue in mice? > > Thanks in advance! > > Inga -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From michael_lafriniere <@t> memorial.org Thu Dec 4 10:40:36 2003 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] In-situ hybridization help please Message-ID: Peter, Before I can respond with the experience I have had with in-situ, may I ask first, which system are you using? Michael LaFriniere PA, HT(ASCP) Pathology Manger Memorial Hospital Chattanooga TN -----Original Message----- From: Peter Munday [mailto:p.munday@inpharmatica.co.uk] Sent: Thursday, December 04, 2003 6:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] In-situ hybridization help please Hello Histonetters, I am currently doing in-situ hybridization on paraffin embedded tissue array sections. Very often I get a signal in the nucleus, which I think is a little odd as I would expect to see it in the cytoplasm. Has anybody else encounted this? If so, is it real? If it isn't real, what can be done about it? Thanks, Peter Munday Inpharmatica London _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From georgecole <@t> ev1.net Thu Dec 4 11:09:47 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Lack of communication of JNEN Message-ID: <000001c3ba89$6cbc1fe0$084dbad0@hppav> Histotechs, This is One-Note George here----in July, when I started offering the packet of videos and pages of muscle and nerve biopsy techniques, I was full of the subjects of histochemistry on those tissues. Now, after about a thousand 2 and 3 and 4 letter processes that I know nothing about showing up day after day on the histonet----I know BVD's but they are my own----and all the new procedures---honest to pete---you histotechs have hoisted yourself up the science ladders to equality with the Med Techs----at least you can snow me even more often than they can. When I started.as a histotech 43 years ago, there was a 3 by 7 foot table strung with tubes and tubules----doing Med Tech Magic----in the middle of the lab where they ruled with magic and test tubes. Med Techs hovered next to the residents in the hierarchy, Vampires, with their needles and histotechs with their paraffins and a hat full of stains huddled busily far down the pecking order. Now there are new machines still buzzing and honking in the med tech's spaces----but the med techs now have to come to the histotechs often to ask you to do things they know nothing about----you must fight the urge to quote that character from one of Dicken's great novels---- ''DON'T KNOW YA, DON'T KNOW YA!!!" Immunos, antibodies, mysterious initials---GLORIOSITY----you histotechs have mysteries enough to wield to snow the neuropathologists themselves!!! It's an honor to call myself one----and a keen observer will note I always have my fingers crossed and stand ready to run for it, when any of you start asking How-To questions. GOOD ON YOU ALL!!! georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031204/ef07b273/attachment.htm From Ben.Shelkowsky <@t> chomp.org Thu Dec 4 11:15:06 2003 From: Ben.Shelkowsky <@t> chomp.org (Shelkowsky, Ben) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D60F0@exchsrvr.chomp.org> The Fontana-Masson silver stain for argentaffin is easy but you must pay attention to the details. Refer to TROUBLESHOOTING HISTOLOGY STAINS by Horobin/Bancroft. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu on behalf of Fearn Tony Sent: Thu 12/4/2003 6:30 AM To: Histonet (E-mail) Cc: Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours We're trying to differentiate between a carcinoid tumour and other neuroendocrine tumours. Grimelius, chromogranin & synaptophysin are positive and we need to be more specific as to the type of tumour, with very little tissue left in the block! I've been told staining for argentaffin granules would be best, but does anyone know which method is the best to go for? Thanks, in advance, for your help. Lesley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From Ben.Shelkowsky <@t> chomp.org Thu Dec 4 11:20:41 2003 From: Ben.Shelkowsky <@t> chomp.org (Shelkowsky, Ben) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D60F1@exchsrvr.chomp.org> One other thing about this subject. I'm sure one of our immuno experts on the Histonet might have some suggestions on a more definitive pathway of staining. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu on behalf of Fearn Tony Sent: Thu 12/4/2003 6:30 AM To: Histonet (E-mail) Cc: Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours We're trying to differentiate between a carcinoid tumour and other neuroendocrine tumours. Grimelius, chromogranin & synaptophysin are positive and we need to be more specific as to the type of tumour, with very little tissue left in the block! I've been told staining for argentaffin granules would be best, but does anyone know which method is the best to go for? Thanks, in advance, for your help. Lesley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From scoop <@t> mail.nih.gov Thu Dec 4 11:27:49 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] microglia In-Reply-To: References: Message-ID: Hi. I don't think there are any that work well in FFPE. Abcam has one that requires proteinasing antigen retrieval that hasn't worked for me. MAC-1 works well in frozen tissue. There's also a special fixation protocol (prior to paraffin embedding) that is supposed to make all the abs that work on frozen tissue work on paraffin embedded tissue - the reference is on the abcam site with their anti-microglial antibody. I know f4/80 works for peripheral macrophages in ffpe tissue, but I don't know if it works on microglia. Another solution would be to use ricin (not the very toxic variety) from Vector (references and protocol on their web site. I would love to find an anti-microglial antibody that is easy to use and works of ffpe tissue but I don't think one exists. Sharon >Hi all! > >Can anyone suggest a good antibody for microglia that works on >frozen and FFPE-tissue in mice? > >Thanks in advance! > >Inga >-- > >Inga Hansson >Dept. neuroscience, >div. neurobiology >PO Box 587, BMC >Husargatan 3 >S-751 23 Uppsala >SWEDEN > >Phone: +46(18) 471 4384 >Fax: +46(18)559017 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From michael_lafriniere <@t> memorial.org Thu Dec 4 11:38:31 2003 From: michael_lafriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Pathologist's Assistant Assistant? Message-ID: Hi Wanda, As PA, Pathology (Manager)& (HT) myself, yes, I require to have a lab assistant, which we call pathology technicians, next to the gross prosector at all times for more than one reason. Two major reasons I do this is; 1. During the afternoon the gross prosector can gross much quicker with assistance to get the tissues on the processors sooner if the lab in under time crunches with processing schedules, which larger labs demonstrate due to many outpatient specimens arriving later in the day, and wanting to give accounts less than 24 hour turn around time. 2. Additional set of eyes is very valuable as a double check system for errors or problems with requisitions, tissue cassettes or special studies/request, etc.. In the long run for the "total process" with histology specimens we found in our lab that produces a variety of 250 specimens per day, that this has eliminated many problems for the next day, in histology, immunohistochemistry, cytology (with non gyn specimens), slides for the pathologists and as well helped the transcriptionists. Overall demonstrating the assistant next to the gross prosector is very valuable. Hope this helped. Michael LaFriniere PA, HT(ASCP) Pathology Manager Memorial Hospital Chattanooga TN 423-495-6117 -----Original Message----- From: Smith Wanda [mailto:Wanda.Smith@HCAhealthcare.com] Sent: Thursday, December 04, 2003 8:51 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathologist's Assistant Assistant? Dear Histonetters, I do not want to open a HUGE can of worms regarding PA's, but I have a question that I need some input on... At facilities that use PA's, is there an Assistant to the Assistant at the grossing table??? In other words, Is there a Lab Assistant or Histotech always available when the PA is cutting? The reason I ask is, we are staffed very thinly, and late in the afternoon, things get busy with the phones, accessioning specimens, late frozen sections, etc, etc and I could use the Lab assistant to do other things rather than stand w/ the PA and change blades... Input on this is appreciated and let me say, I do not want to open the "Let's bash the PA's" can of worms!!! Thanks, Wanda > Wanda G. Smith, HTL/HT(ASCP) > Pathology Supervisor > Trident Medical Center Laboratory Services > *9330 Medical Plaza Drive, Charleston, SC 29406 > *843-797-4586 *fax 843-797-4296 > *wanda.smith@hcahealthcare.com > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Dec 4 11:58:27 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours Message-ID: What is the tissue source? I would think the good old "H&E" and clinical history (hormone levels) would be your best bet. I have found IHC for serotonin to be helpful in identifying midgut carcinoid tumors. IHC for insulin, glucagon, and somatostatin can be helpful for islet cell tumors. Richard Cartun >>> "Shelkowsky, Ben" 12/04/03 12:20PM >>> One other thing about this subject. I'm sure one of our immuno experts on the Histonet might have some suggestions on a more definitive pathway of staining. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu on behalf of Fearn Tony Sent: Thu 12/4/2003 6:30 AM To: Histonet (E-mail) Cc: Subject: [Histonet] Differentiating between carcinoid & other neuroendocrine tumours We're trying to differentiate between a carcinoid tumour and other neuroendocrine tumours. Grimelius, chromogranin & synaptophysin are positive and we need to be more specific as to the type of tumour, with very little tissue left in the block! I've been told staining for argentaffin granules would be best, but does anyone know which method is the best to go for? Thanks, in advance, for your help. Lesley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Thu Dec 4 12:39:44 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] CureMount Mounting Medium In-Reply-To: <007201c3b9d3$08578fa0$6401a8c0@instrumedics1> Message-ID: Bernice, Does this mounting media compensate for staining of the coating on your coated slides for tape sections? Especially with IHC the coating on the slide picks up chromogen in my experience. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Instrumedics Sent: Wednesday, December 03, 2003 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CureMount Mounting Medium Instrumedics' CureMount Mounting Medium is designed for use with dehydrated and cleared sections. The cover slipped slide is placed under a UV light for 20 seconds which converts the "liquid" into a "solid" with a refractive index of 1.55 which matches the refractive index of the section. When viewed in the microscope the stained elements stand out and the background becomes virtually invisible. No more sticking to the slide tray or to a neighboring slide. The slide is "dry" and can be archived immediately. Bernice schiller@instrumedics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> earthlink.net Thu Dec 4 12:39:11 2003 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] BioCare in the Steamer In-Reply-To: <20031204143500.21467.76321.Mailman@swlx167.swmed.edu> References: <20031204143500.21467.76321.Mailman@swlx167.swmed.edu> Message-ID: <6.0.0.22.0.20031204133421.029b8ab0@127.0.0.1> Patsy, We have used Borg Decloaker in the steamer. It worked fine. They have a good product. The built in pH indicator is a great concept. While we don't use it much it worked fine when we did and we had no trouble with it in the steamer. Amos Brooks At 09:35 AM 12/4/03, you wrote: >Has anybody tried the AR reagents that deparaffinzed such as BioCare >Decloaker in a steamer in place of the pressure cooker? PC is too harsh for >some of my stuff but I like using these retrieval/deparaffination reagents. >Patsy From amosbrooks <@t> earthlink.net Thu Dec 4 12:43:46 2003 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] BioCare in the steamer In-Reply-To: <20031204143500.21467.76321.Mailman@swlx167.swmed.edu> References: <20031204143500.21467.76321.Mailman@swlx167.swmed.edu> Message-ID: <6.0.0.22.0.20031204133946.029b8740@127.0.0.1> Oh, By the way, We didn't deparaffinize with it (the Borg Decloaker). We used xylene and ethanol then used the product like the other retrieval products we have. Deparaffinizing and retrieving in the same solution feels like scratching a chalk board to me. Amos From info <@t> instrumedics.com Thu Dec 4 14:09:55 2003 From: info <@t> instrumedics.com (Instrumedics) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Re:Instrumedics video request References: <20031204180001.13214.6900.Mailman@swlx167.swmed.edu> Message-ID: <000801c3baa2$96391ee0$6401a8c0@instrumedics1> Histonetters, Would all those who no long need the Instrumedics video please kindly return it to Instrumedics so it can be passed on to others! Thank you. Bernice schiller@instrumedics.com From ljb <@t> medicine.wisc.edu Thu Dec 4 16:13:09 2003 From: ljb <@t> medicine.wisc.edu (LaCinda Burchell) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] BioCare in the Steamer Message-ID: We use the Biocare Decloaker for all of our HIER. It works great! Cindy B LaCinda Burchell, BA, AS, HT(ASCP) University of Wisconsin-Madison, Medical School Asthma and Allergy Research IHC Lab 600 Highland Ave. CSC K4/913 Madison, Wisconsin 53792 Phone: 608-262-3518 FAX: 608-263-3746 >>> Amos Brooks 12/04/03 12:39PM >>> Patsy, We have used Borg Decloaker in the steamer. It worked fine. They have a good product. The built in pH indicator is a great concept. While we don't use it much it worked fine when we did and we had no trouble with it in the steamer. Amos Brooks At 09:35 AM 12/4/03, you wrote: >Has anybody tried the AR reagents that deparaffinzed such as BioCare >Decloaker in a steamer in place of the pressure cooker? PC is too harsh for >some of my stuff but I like using these retrieval/deparaffination reagents. >Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lesley <@t> vancouverbc.net Thu Dec 4 16:51:41 2003 From: lesley <@t> vancouverbc.net (Lesley Weston) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] 200 proof absolute ethyl alcohol In-Reply-To: Message-ID: I've always used graded ethyl alcohol for processing, finishing up with absolute before going into the clearing agent. Perhaps your times in each grade were too long, especially the 70% and the absolute. Lesley Weston. on 01/12/2003 10:30 AM, Tague, Curtis at CTague@ahs.llumc.edu wrote: > i remember using some of this stuff many years ago to process tissue and ended > up with a bunch of fried or burnt looking tissue. i was told ethyl alcohol is > not good for processing tissue but i think i've heard different since then. > anyone have any comments? can ethyl alcohol be used to process tissue and the > error was a result of some other mistake? > > > thanks for your help, > curt > > Confidentiality Note: > > The preceding e-mail message (including any attachments) contains information > that may be confidential, protected by applicable legal privileges, or > constitute non-public information. It is intended to be conveyed only to the > designated recipient(s). If you are not an intended recipient of this message, > please notify the sender by replying to this message and then delete it from > your system. Use, dissemination, distribution or reproduction of this message > by unintended recipients is not authorized and may be unlawful. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Dec 4 17:40:27 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] In-situ hybridization help please Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0E9@simba.kids> Peter, You may find that the staining corresponds to the nucleolus. I have found that with many mRNA-ISH reactions, if there is cytoplasmic staining then there is often nucleoloar staining as well. Not surprising since mRNA is initially produced in the nucleus. I will upload to Histonet some pics on this type of staining shortly. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Peter Munday [mailto:p.munday@inpharmatica.co.uk] Sent: Thursday, 4 December 2003 10:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] In-situ hybridization help please Hello Histonetters, I am currently doing in-situ hybridization on paraffin embedded tissue array sections. Very often I get a signal in the nucleus, which I think is a little odd as I would expect to see it in the cytoplasm. Has anybody else encounted this? If so, is it real? If it isn't real, what can be done about it? Thanks, Peter Munday Inpharmatica London _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From georgecole <@t> ev1.net Thu Dec 4 23:38:12 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] HOW ARE THE MUSCLE/NERVE DVD'S HOLDING UP? Message-ID: <000001c3baf1$fa3ecc10$044dbad0@hppav> Histotechs who have received muscle and nerve packet---- How are the DVD's holding up? I hear they play all right in the USA and over seas. I know the disc material is a bit soft, so we have to handle them carefully, but mine have stood up through quite a bit of playing----anybody find differently? georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031204/c32537f1/attachment.htm From lpwenk <@t> covad.net Fri Dec 5 04:01:17 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Stain for H.Pylori References: <20031203165805.14533.qmail@web40905.mail.yahoo.com> <003701c3b9ca$1b7e4be0$7504a8c0@um.edu> Message-ID: <007401c3bb16$ba0e9ba0$8732fea9@hppav> How does one make up the "stock" Giemsa, which is then diluted to make the working? - concentration? (X grams Giemsa powder to Y grams solvent?) - pH? (with what - acetic acid? buffer? Which one?) - aqueous or alcoholic? - if alcoholic, what %?? which alcohol? (ethanol? methanol?) - etc. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: Hernan Aldana Marcos To: Jill Cox Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 03, 2003 1:20 PM Subject: Re: [Histonet] Stain for H.Pylori MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER FIXATION: Neutral Formolin 10% SECCIONS: 4-5 ?m SOLUTIONS: ACETIC WATER Acetic acid............... 0,01 ml Destilated water............... 1000,00 ml Working solution GIEMSA Giemsa.............. 10,0 ml Acetic water.............. 50,0 ml PROCEDDURE 1. Take slides to water destiled 2. Giemsa working solution 1 h 3. Deshidratation in 3 changes of alcohol 100 4. xilol, syntetic mounting medium RESULTADOS H. pylorii.............. intense blue Nuclei.............. blue Cytoplasm.............. Rose REFERENCE Luna L.: "Wet work shop for special Alabama" Noviembre 2 de 1984 Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: Jill Cox To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 03, 2003 1:58 PM Subject: [Histonet] Stain for H.Pylori Hello everyone, Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it? Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab ---------------------------------------------------------------------------- Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/dda49e0c/attachment.htm From syedab <@t> totalise.co.uk Fri Dec 5 06:08:01 2003 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Immochem References: <20031203165805.14533.qmail@web40905.mail.yahoo.com> <003701c3b9ca$1b7e4be0$7504a8c0@um.edu> <007401c3bb16$ba0e9ba0$8732fea9@hppav> Message-ID: <004a01c3bb28$6e55ab60$80c401a3@clneuro.ox.ac.uk> Dear All, Does anyone have the contact details of Immochem, New Zealand? I can't find a homepage for them on the internet anywhere. Any help would be greatly appreciated Anila Syed --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.547 / Virus Database: 340 - Release Date: 02/12/03 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/e6e58375/attachment.htm From rfail <@t> toolkitmail.com Fri Dec 5 06:33:01 2003 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Stain for H.Pylori Message-ID: <3fd07afd.259.388a.317636285@toolkitmail.com> Peggy, There is a slightly different Giemsa method in Histologic for H. pyloric using a microwave. Rena Fail How does one make up the "stock" Giemsa, which is then > diluted to make the working? - concentration? (X grams > Giemsa powder to Y grams solvent?) - pH? (with what - > acetic acid? buffer? Which one?) - aqueous or alcoholic? > - if alcoholic, what %?? which alcohol? (ethanol? > methanol?) - etc. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > ----- Original Message ----- > From: Hernan Aldana Marcos > To: Jill Cox > Cc: histonet@lists.utsouthwestern.edu > Sent: Wednesday, December 03, 2003 1:20 PM > Subject: Re: [Histonet] Stain for H.Pylori > > > MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER > > FIXATION: Neutral Formolin 10% > > SECCIONS: 4-5 ?m > > > > SOLUTIONS: > > ACETIC WATER > > Acetic acid............... 0,01 ml > > Destilated water............... 1000,00 ml > > > > Working solution GIEMSA > > Giemsa.............. 10,0 ml > > Acetic water.............. 50,0 ml > > > > PROCEDDURE > > 1. Take slides to water destiled > > 2. Giemsa working solution 1 h > > 3. Deshidratation in 3 changes of alcohol 100 > > 4. xilol, syntetic mounting medium > > > > RESULTADOS > > H. pylorii.............. intense blue > > Nuclei.............. blue > > Cytoplasm.............. Rose > > > > REFERENCE > > Luna L.: "Wet work shop for special Alabama" Noviembre 2 > de 1984 > > > Dr. Hern?n J. Aldana Marcos > Facultad de Medicina. Universidad de Mor?n > Machado 914. B1708JPD. Buenos Aires. Argentina > e-mail alternativo hernanjavier@yahoo.com > web: http://hjaldanamarcos.bravepages.com > http://histologia.bigthicketdirectory.net/main.html > > ----- Original Message ----- > From: Jill Cox > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, December 03, 2003 1:58 PM > Subject: [Histonet] Stain for H.Pylori > > > Hello everyone, > Has anyone done a Toluidine blue/Alcian yellow stain > for H. Pylori? If so how do the Doctors like it? Or, > what other stains are you doing for that. I am trying to > get away from the Genta!! Thanks in advance > > > > Jill Cox HT (ASCP) > Histology Supervisor > Seattle Histology Lab > > > ---------------------------------------------------------- > ------------------ > Do you Yahoo!? > Free Pop-Up Blocker - Get it now > From NSEARCY <@t> swmail.sw.org Fri Dec 5 06:32:40 2003 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Molecular Morphology Meeting Message-ID: <03Dec5.063310cst.87328@healthcare.sw.org> Can anyone in histo land recommend meetings dealing with the above. I have employees wanting basic, intermediate as well as advanced learning. Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Temple, Texas Division Manager, Anatomic Pathology 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita END:VCARD From stevemachinuk <@t> yahoo.co.uk Fri Dec 5 08:10:38 2003 From: stevemachinuk <@t> yahoo.co.uk (=?iso-8859-1?q?Steve=20Machin=20UK?=) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains Message-ID: <20031205141038.33372.qmail@web25101.mail.ukl.yahoo.com> Could anyone help us with a problem we have in processing very soft fetal brains? We currently fix in 20% formalin in buffer but the brains are so soft after processing they stick to the wrapping paper. Any ideas on how we can harden them after fixation? Best Wishes Steve Machinu UK ________________________________________________________________________ Download Yahoo! Messenger now for a chance to win Live At Knebworth DVDs http://www.yahoo.co.uk/robbiewilliams From mcauliff <@t> umdnj.edu Fri Dec 5 09:22:20 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains In-Reply-To: <20031205141038.33372.qmail@web25101.mail.ukl.yahoo.com> References: <20031205141038.33372.qmail@web25101.mail.ukl.yahoo.com> Message-ID: <3FD0A2AC.7040400@umdnj.edu> Hi Steve: How long are you fixing the brains? 48 hours is the minimum for formalin, no matter what the concentration. One week is not too long. How big are the brains? Fetal mose brain is easier to fix by immersion than a fetal horse brain. Size does matter. Are you sure your formalin is good? Geoff Steve Machin UK wrote: >Could anyone help us with a problem we have in processing very soft >fetal brains? > >We currently fix in 20% formalin in buffer but the brains are so soft >after processing they stick to the wrapping paper. > >Any ideas on how we can harden them after fixation? > >Best Wishes >Steve Machinu UK > > >________________________________________________________________________ >Download Yahoo! Messenger now for a chance to win Live At Knebworth DVDs >http://www.yahoo.co.uk/robbiewilliams > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Terry.Marshall <@t> rothgen.nhs.uk Fri Dec 5 09:33:48 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains Message-ID: Steve Machin UK wrote: >Could anyone help us with a problem we have in processing very soft >fetal brains? > >We currently fix in 20% formalin in buffer but the brains are so soft >after processing they stick to the wrapping paper. > >Any ideas on how we can harden them after fixation? I find zinc fixative totally brilliant for brain tissue, in both the hardening and morphologic aspects. You may not be able to keep the tissue on the slide, but heck, you can't have everything:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From fmonson <@t> wcupa.edu Fri Dec 5 10:02:05 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains Message-ID: Morning Steve, Well, ---____..... Oh, I can't do this. The temptation to humor is really strong on a Friday, but Ill try to detrain myself from this sticky morsel. Thus, My approach would be to determine the reason for the absence of hardening. Two possibilities come to mind. 1. Very high phospholipid content which might interfere with diffusion of HCHO. 2. Very high GAG content which might leave residual 'stickiness'. I like this better!* Solutions Lower the temp at which you handle tissues. Add GAG 'precipitators' to fixative, i.e. cetylpyridinium chloride(?) Use wax paper instead of wrapping paper, it's just more paraffin. *I'm overcome with the notion that the cranium is first filled with ground substance that serves as both nutrient and matrix for later development. This latter idea may imply to some that I have missed some parts of the latter stages of development and am, thus, using residual ground substance for dreaming up such 'curious' suggestions. Of course, if one spends a lifetime promulgating curious ideas, one is bound to hear complaints that they are grounded in no substance at all. Finally, I hope for your sake that John Kiernan is awake and thinking, with his highly developed substance, about your problem. He is one who has been trained in that chapter of the Histology texts that is often skipped for lack of time in the normal courses of histo-events. Cheers, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Steve Machin UK [mailto:stevemachinuk@yahoo.co.uk] Sent: Friday, December 05, 2003 9:11 AM To: Histonet Histonet Histonet Subject: [Histonet] Hardening very soft brains Could anyone help us with a problem we have in processing very soft fetal brains? We currently fix in 20% formalin in buffer but the brains are so soft after processing they stick to the wrapping paper. Any ideas on how we can harden them after fixation? Best Wishes Steve Machinu UK ________________________________________________________________________ Download Yahoo! Messenger now for a chance to win Live At Knebworth DVDs http://www.yahoo.co.uk/robbiewilliams _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> gmhsc.com Fri Dec 5 10:17:28 2003 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] test Message-ID: Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mlm11 <@t> cornell.edu Fri Dec 5 10:23:00 2003 From: mlm11 <@t> cornell.edu (Mary Lou) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] eugenol questions Message-ID: <5.2.1.1.2.20031205110613.02f9ad88@postoffice9.mail.cornell.edu> http://ntmain.utb.edu/lcabrera/4420/safranin-frastgreen_sllide_staining.html Dear Histonetters, I had never heard of eugenol before the above procedure. Could someone explain the importance of it please? My search has not shown a histology connection, just use in perfume, as a dental analgesic, and insect attractant. Thanks, Mary Lou Norman College of Veterinary Medicine Cornell From kemlo <@t> tiscali.co.uk Fri Dec 5 10:23:04 2003 From: kemlo <@t> tiscali.co.uk (Kemlo Rogerson) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains In-Reply-To: Message-ID: <000501c3bb4c$19845f40$97d4e150@KEMLOS> Yes I remember that with much pain; zinc, lead, you name it we fixed with it! If my memory serves me it made the nuclear stain rather dramatic too. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 01270 877625 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts:? ???????????? kemlo@tiscali.co.uk?or kemlo1@btinternet.com? Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. ? ? ? -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: 05 December 2003 15:34 To: Geoff McAuliffe; Steve Machin UK Cc: Histonet Histonet Histonet Subject: RE: [Histonet] Hardening very soft brains Steve Machin UK wrote: >Could anyone help us with a problem we have in processing very soft >fetal brains? > >We currently fix in 20% formalin in buffer but the brains are so soft >after processing they stick to the wrapping paper. > >Any ideas on how we can harden them after fixation? I find zinc fixative totally brilliant for brain tissue, in both the hardening and morphologic aspects. You may not be able to keep the tissue on the slide, but heck, you can't have everything:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Dec 5 10:26:31 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains Message-ID: Don't exaggerate Kemlo, we didn't use lead as a fixative. However we stained with lead haematoxylin (upside down) for APUD granules as they were then called, in carcinoids. BTW, for those not familiar with baby brains, they are like blancmange, or even runnier - horrid things to handle. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo@tiscali.co.uk] Sent: 05 December 2003 16:23 To: Marshall Terry Dr, Consultant Histopathologist; 'Geoff McAuliffe'; 'Steve Machin UK' Cc: 'Histonet Histonet Histonet' Subject: RE: [Histonet] Hardening very soft brains Yes I remember that with much pain; zinc, lead, you name it we fixed with it! If my memory serves me it made the nuclear stain rather dramatic too. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 01270 877625 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts:? ???????????? kemlo@tiscali.co.uk?or kemlo1@btinternet.com? Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. ? ? ? -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: 05 December 2003 15:34 To: Geoff McAuliffe; Steve Machin UK Cc: Histonet Histonet Histonet Subject: RE: [Histonet] Hardening very soft brains Steve Machin UK wrote: >Could anyone help us with a problem we have in processing very soft >fetal brains? > >We currently fix in 20% formalin in buffer but the brains are so soft >after processing they stick to the wrapping paper. > >Any ideas on how we can harden them after fixation? I find zinc fixative totally brilliant for brain tissue, in both the hardening and morphologic aspects. You may not be able to keep the tissue on the slide, but heck, you can't have everything:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> gmhsc.com Fri Dec 5 10:38:47 2003 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Bone Marrow Biopsy Message-ID: Hello Histonetters ! Hope that everyone had a great Thanksgiving.; I was wandering how all of you netters fixed and decaled your bone marrow biopsy specimens? Here lately the doc that reads out our BM cases says that the biopsy looks kind of smudgy gray. What could this be? He wants to believe that it is not getting deparaffinized enough but all of the other slides in the rack are just fine..... I am open for any suggestions.... Thanks in advance Amy Self Georgetown Memorial Hospital Georgetown, SC 29440 843-527-7179 (work) 843-520-7882 (fax) Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Loralee_Gehan <@t> URMC.Rochester.edu Fri Dec 5 10:46:32 2003 From: Loralee_Gehan <@t> URMC.Rochester.edu (Gehan, Loralee) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] fixed frozen IHC Message-ID: <95774A6A6036D411AFEA00D0B73C864308880495@exmc3.urmc.rochester.edu> I think that this was already discussed a few weeks ago so sorry for the repeat. I need to do some immunohistochemistry on fixed (some in gluteraldehyde, so in formalin) frozen sections. Does anyone have any tricks to make some of the more tricky antibodies work? Suggestions for Antigen retrieval? Thanks in advance. Loralee Gehan University of Rochester Orthopaedics Research Department From leopold <@t> mnsi.net Fri Dec 5 10:54:20 2003 From: leopold <@t> mnsi.net (Derek and/or Lynda Leopold) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] workflow survey Message-ID: <3FD0B83C.3080905@mnsi.net> Hi H'netters, I am a brand new Histotech working out of a hospital in Detroit. We are currently experiencing difficulties regarding inadequately processed blocks as well as crazy workload bottlenecks due to the Pathologists' tendencies to treat processing times like buses--miss the proper one and it's ok because another will come along in a couple of hours--...Of course, this is being heaped upon the cutting techs as poor workflow management (ie we're obviously working too slowly). We have convinced the powers that be to do a statistical study of work output but now are wondering how best to go about it. We do suspect that caseloads not getting to the proper early processing runs are the major cause of the problem, but just to be sure, we want a way to measure and compare embedding/trimming/cutting times. An example is: should one treat biopsies as bigger "units" since they require levels and one slide takes longer on average than a bigger piece of tissue? Any suggestions you may offer would surely be appreciated. Thanks, and really thrilled to be a Histotech, finally, Lynda Leopold From peoshel <@t> wisc.edu Fri Dec 5 11:13:26 2003 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains In-Reply-To: References: Message-ID: It's Friday, I have to ... So do they turn everyone into Scotsmen, plotting to win Wimbledon? Phil >BTW, for those not familiar with baby brains, they are like >blancmange, or even runnier - horrid things to handle. > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From Stephen.J.Scholz <@t> osfhealthcare.org Fri Dec 5 11:32:06 2003 From: Stephen.J.Scholz <@t> osfhealthcare.org (Scholz, Stephen J.) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] pretreatment for IHC Message-ID: <7F1312711CA7474A89B3DF8BA0BA54D0F5F0A0@pmc-rfd-mx01.intranet.osfnet.org> Hello Histonet, I am having a problem with background staining on IHC slides. This is only happening in cell block specimens. Is this common on cell blocks? Should I do a pretreatment on these? What pretreatments do others prefer? Should I peroxide quench? Before or after? What about blockers? Do I ramble on and ask to many questions? Thanks for all your help, Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 e-mail: sjscholz@osfhealthcare.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/8c6542b9/attachment.htm From haldana <@t> unimoron.edu.ar Fri Dec 5 11:53:02 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] fixed frozen IHC References: <95774A6A6036D411AFEA00D0B73C864308880495@exmc3.urmc.rochester.edu> Message-ID: <010201c3bb58$a24bd220$7504a8c0@um.edu> You have a lot of advaices in the wonderfull manual of Technical Immunohistochemistry of Rodney Miller, go to http://www.propathlab.com and download it free.Or go to "http://hjaldanamarcos.bravepages.com/documentos.htm" and download the documents named INMUNO1.... to INMUNO5. Be patient becouse this web pages are free and if a lot of people download the archives the pages interrupt temporarly (continue in other day) Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: "Gehan, Loralee" To: Sent: Friday, December 05, 2003 1:46 PM Subject: [Histonet] fixed frozen IHC > I think that this was already discussed a few weeks ago so sorry for the > repeat. > > I need to do some immunohistochemistry on fixed (some in gluteraldehyde, so > in formalin) frozen sections. Does anyone have any tricks to make some of > the more tricky antibodies work? Suggestions for Antigen retrieval? > Thanks in advance. > > Loralee Gehan > University of Rochester > Orthopaedics Research Department > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mlm11 <@t> cornell.edu Fri Dec 5 12:02:34 2003 From: mlm11 <@t> cornell.edu (Mary Lou) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] eugenol questions Message-ID: <5.2.1.1.2.20031205124909.02f6ad78@postoffice9.mail.cornell.edu> Thanks, George. Now the next question should be how critical is it and do you suppose it can be left out of the procedure I listed below? Thanks, again........Mary Lou >Mary Lou; >Eugenol---clove oil---was a common reagent in the old days----see Gray's >Microtomists Formulary and Guide----it was a favorite clearing agent, >and it was used as a solvent in many a classical mixture, --and it was >used on nitrocellulose preparations, and for sectioning them. John >Kiernon will be along any minute with the additional 999 uses. >geoergecole@ev1.net > >-----Original Message----- >From: histonet-admin@lists.utsouthwestern.edu >[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Mary Lou >Sent: Friday, December 05, 2003 8:23 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] eugenol questions > > >http://ntmain.utb.edu/lcabrera/4420/safranin-frastgreen_sllide_staining. >html > >Dear Histonetters, >I had never heard of eugenol before the above procedure. Could someone >explain the importance of it please? My search has not shown a >histology >connection, just use in perfume, as a dental analgesic, and insect >attractant. > >Thanks, >Mary Lou Norman >College of Veterinary Medicine >Cornell > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Dec 5 12:14:27 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Re: Eugenol questions References: <5.2.1.1.2.20031205110613.02f9ad88@postoffice9.mail.cornell.edu> Message-ID: <3FD0CB03.95352A44@uwo.ca> The web site quoted in Mary Lou's email gives a procedure for staining paraffin sections with safranine O followed by fast green FCF. There is no statement about fixation, expected results or what tissue to use it on. Probably the most frequent use of this dye combination is as a general oversight method for vascular plant tissues. It colours xylem and nuclei red, and cellulose & cytoplasm green. The same combination can be used (after chromic-osmium fixation) for chromosomes and other classical cytological demonstrations (see M.Gabe, "Histological Techniques," 1976 for discussion). The method described in the web page resembles the method of Sass (1958; cited from Ruzin's 1999 book, "Plant Microtechnique and Microscopy") but it has two unusual features: 1. Treatment with 1% CrO3 after staining with safranine; said to improve retention of the dye. (In Gray's "Formulary and Guide" there are several methods in which chromic acid is applied to sections before staining, but I couldn't find one where it was used after.) 2. A strange and illogical procedure for dehydrating and clearing the stained sections. This involves two mixtures containing different proportions of eugenol, xylene and ethanol. Eugenol is the chief ingredient (85%) of clove oil, which has many traditional uses in microscopy: as clearing agent, solvent for dyes, differentiator etc. It was often used as a clearing agent in earlier times when 100% alcohol was not easily available, because it's miscible with 90% ethanol (see Gray, p.56 etc). Eugenol has a high refractive index (1.54), which makes for good transparency in whole-mount preparations (cf clove oil 1.53, xylene 1.50; data from the Merck Index). In the staining method on that web site, the eugenol-xylene-alcohol mixtures are applied after complete dehydration in "abs. EtOH," and are followed by a 10:1 xylene-alcohol mixture and final clearing in 2 lots of xylene. Thus, the eugenol is not needed for dehydration (already thoroughly done), and it is all throughly removed from the section before mounting in permount (so no possible effect on refractive index of the final preparation). This has the look of a method that has been passed down through generations of people who were not thinking about what they were doing! There are plenty of sensible safranine-fast green methods. All are slightly tricky because the second dye can remove too much of the first. A very simple procedure is described in great detail on pp.97-98 of Berlyn & Miksche, "Botanical Microtechnique and Cytochemistry" Ames: Iowa State Univ Press (1976). This 326-page hardback can be bought new for a very low price from the publisher. Ruzin (1999) describes this method more briefly, and also gives a more complicated one, which I have not tried myself. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ------------------------- Mary Lou wrote: > > http://ntmain.utb.edu/lcabrera/4420/safranin-frastgreen_sllide_staining.html > > Dear Histonetters, > I had never heard of eugenol before the above procedure. Could someone > explain the importance of it please? My search has not shown a histology > connection, just use in perfume, as a dental analgesic, and insect attractant. > > Thanks, > Mary Lou Norman > College of Veterinary Medicine > Cornell From MTitford <@t> aol.com Fri Dec 5 12:22:47 2003 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Foetal/Fetal brain hardening. Message-ID: <0ABE4903.4E9FA568.00762DB1@aol.com> Steve Machine asks about hardening of fetal brains. We had this problem once with soft mushy brains. We partially solved it by washing the brain all day in running water, then infiltrating the brain with increasing strengths of gelatin at 37 degrees centigrade. (For example 0.5%, 2% and then 5%)over a couple of days. Then we drained off the gelatin and fixed it again for a couple of days. This added some firmness to the tissue. A disadvantage was it added some basophilia to the staining, but it was worth it. Mike Titford USA Pathology Mobile AL USA From kspencer <@t> utmem.edu Fri Dec 5 13:18:35 2003 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] pretreatment for IHC In-Reply-To: <7F1312711CA7474A89B3DF8BA0BA54D0F5F0A0@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: I can only tell you that until I started washing in 0.1% Triton in PBS was I able to eliminate background, but this is on 20 micron tissue sections. Of course, you have to peroxide quench before, and you have to block with normal serum from the same species as your your secondary Ab. IE if you have goat anti mouse secondary, then normal goat serum. Hope this helps. -Kathleen On Friday, December 5, 2003, at 11:32 AM, Scholz, Stephen J. wrote: > Hello Histonet, > > I am having a problem with background staining on IHC slides.? This is > only happening in cell block specimens.? Is this common on cell > blocks?? Should I do a pretreatment on these?? What pretreatments do > others prefer?? Should I peroxide quench?? Before or after?? What about > blockers?? Do I ramble on and ask to many questions? > > Thanks for all your help, > > Stephen J. Scholz HT(ASCP) > Histology Coordinator > OSF St. Anthony Medical Center > Rockford IL > > Phone: 815-395-5410 > Fax: 815-395-5364 > e-mail: sjscholz@osfhealthcare.org > -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: text/enriched Size: 1345 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/c761146c/attachment.bin From siksik03 <@t> comcast.net Fri Dec 5 13:25:48 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains In-Reply-To: <3FD0A2AC.7040400@umdnj.edu> References: <20031205141038.33372.qmail@web25101.mail.ukl.yahoo.com> <3FD0A2AC.7040400@umdnj.edu> Message-ID: Hi HistoNetters Kok and Boon describe a technique, on pp. 116-118 of the newest edition of their book, which is a combination of microwave stabilization in saline (two steps) and two fixation steps (one "soaking step", on the bench, in 10% NBF, and one true microwave fixation step, also in 10% NBF). Thsi technique was first published in: Boon ME, Marani E. Adriolo PJM, Steffelaar JW, Bots GTAM, Kok LP (1988) Microwave irradiation of human brains: Production of microscopic slides within one day, J Clin Pathol 41: 590-593 Adult human brains can take weeks to be adequately fixed by conventional methods. This work was done using a BioRad H2500 microwave, now produced by Energy Beam Sciences. I have done similar work more recently using a Hacker/Milestone microwave, and I can attest to the simplicity and consistency of the method. With foetal brains, the times can, of course, be shortened, but the principles remain the same (stabilize, cut, soak, then microwave fix). best regards, Steven Slap At 10:22 AM -0500 12/5/03, Geoff McAuliffe wrote: >Hi Steve: > >How long are you fixing the brains? 48 hours is the minimum for >formalin, no matter what the concentration. One week is not too long. >How big are the brains? Fetal mose brain is easier to fix by >immersion than a fetal horse brain. Size does matter. >Are you sure your formalin is good? > >Geoff > >Steve Machin UK wrote: > >>Could anyone help us with a problem we have in processing very soft >>fetal brains? >> >>We currently fix in 20% formalin in buffer but the brains are so soft >>after processing they stick to the wrapping paper. >> >>Any ideas on how we can harden them after fixation? >> >>Best Wishes >>Steve Machin UK From cormier <@t> MIT.EDU Fri Dec 5 13:39:47 2003 From: cormier <@t> MIT.EDU (Kathleen Cormier) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hamster tumor differentiation Message-ID: <5.0.2.1.2.20031205143249.00abc388@hesiod> Hello All1 I was wondering, does anyone have any suggestions on where to start for IHC staining of a FFPE hamster tumor? One of our researchers thinks that it might be a mesenchymal type tumor. What panels do people use in clinical work? Any suggestions for a literature (book, article etc) reference for clinical panels, currently in use? (Even if in humans?) It's been a long while for me since I have had to think about clinical work. Thanks so much!! Kathy Cormier DCM_MIT From garygill <@t> dcla.com Fri Dec 5 13:49:19 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Hardening very soft brains Message-ID: For parents of teenagers, it's more often a matter of softening very hard brains. Gary Gill -----Original Message----- From: Steven E. Slap [mailto:siksik03@comcast.net] Sent: Friday, December 05, 2003 2:26 PM To: Steve Machin UK Cc: Histonet Histonet Histonet; cgaspari@ebsciences.com Subject: Re: [Histonet] Hardening very soft brains Hi HistoNetters Kok and Boon describe a technique, on pp. 116-118 of the newest edition of their book, which is a combination of microwave stabilization in saline (two steps) and two fixation steps (one "soaking step", on the bench, in 10% NBF, and one true microwave fixation step, also in 10% NBF). Thsi technique was first published in: Boon ME, Marani E. Adriolo PJM, Steffelaar JW, Bots GTAM, Kok LP (1988) Microwave irradiation of human brains: Production of microscopic slides within one day, J Clin Pathol 41: 590-593 Adult human brains can take weeks to be adequately fixed by conventional methods. This work was done using a BioRad H2500 microwave, now produced by Energy Beam Sciences. I have done similar work more recently using a Hacker/Milestone microwave, and I can attest to the simplicity and consistency of the method. With foetal brains, the times can, of course, be shortened, but the principles remain the same (stabilize, cut, soak, then microwave fix). best regards, Steven Slap At 10:22 AM -0500 12/5/03, Geoff McAuliffe wrote: >Hi Steve: > >How long are you fixing the brains? 48 hours is the minimum for >formalin, no matter what the concentration. One week is not too long. >How big are the brains? Fetal mose brain is easier to fix by >immersion than a fetal horse brain. Size does matter. >Are you sure your formalin is good? > >Geoff > >Steve Machin UK wrote: > >>Could anyone help us with a problem we have in processing very soft >>fetal brains? >> >>We currently fix in 20% formalin in buffer but the brains are so soft >>after processing they stick to the wrapping paper. >> >>Any ideas on how we can harden them after fixation? >> >>Best Wishes >>Steve Machin UK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Dec 5 14:11:47 2003 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Bone Marrow Biopsy Message-ID: <9F4410664F56DC4A8B2BD42920756D0C490CB5@fh2k093.fhmis.net> What fixative are you using? We use B-plus (a new fixative replacing B5) from BCC. Immunos look good with this as well. -----Original Message----- From: Amy Self [mailto:ASelf@gmhsc.com] Sent: Friday, December 05, 2003 11:39 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Bone Marrow Biopsy Hello Histonetters ! Hope that everyone had a great Thanksgiving.; I was wandering how all of you netters fixed and decaled your bone marrow biopsy specimens? Here lately the doc that reads out our BM cases says that the biopsy looks kind of smudgy gray. What could this be? He wants to believe that it is not getting deparaffinized enough but all of the other slides in the rack are just fine..... I am open for any suggestions.... Thanks in advance Amy Self Georgetown Memorial Hospital Georgetown, SC 29440 843-527-7179 (work) 843-520-7882 (fax) Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From SDSU_HISTO <@t> SDSTATE.EDU Fri Dec 5 14:13:59 2003 From: SDSU_HISTO <@t> SDSTATE.EDU (SDSU HISTO) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) Message-ID: LABORATORY TECHNICIAN - VETERINARY SCIENCE This is a full-time, temporary position to last approximately three (3) months. Salary Range N11: $9.39 per hour POSITION PURPOSE: To prepare histology and immunohistochemistry slides for pathologic examination by faculty and to assist with general laboratory maintenance such as filing microscope slides and blocks, cleaning equipment and loading the tissue processor or automated stainers. KNOWLEDGE, SKILLS AND ABILITIES: Knowledge of: * methods, materials, equipment and techniques of laboratory testing, analysis and media preparation; * basic principles, practices, and procedures of laboratory testing; * laboratory equipment, procedures, operation, and terminology; and * safe laboratory practices; Skill and ability to: * perform basic microtome; * prioritize work; * follow detailed directions and instructions; * use and maintain laboratory equipment; * perform laboratory tests or analysis; * accurately record data onto computer; and * lift up to 50 lbs. COMMENTS: Some work with potentially noxious chemicals is performed in the laboratory under a class II fume hood. Deadline: Open Until Filled Submit SD Job Application Form to: SDSU Human Resources Box 2201 Brookings, SD 57007 (605) 688-4128 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/536a96ac/attachment.htm From Ronnie_Houston <@t> bshsi.com Fri Dec 5 15:04:39 2003 From: Ronnie_Houston <@t> bshsi.com (Houston, Ronnie) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota St ate University) Message-ID: <530361BF03351B4CAE5270A05D3037B5FE0585@bsrexms01.BSHSIR.COM> At that salary, the position will stay open for a long, long time Ronnie -----Original Message----- From: SDSU HISTO [mailto:SDSU_HISTO@SDSTATE.EDU] Sent: Friday, December 05, 2003 3:14 PM To: 'histonet@lists.utsouthwestern.edu' Cc: 'fquin@hotmail.com' Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) LABORATORY TECHNICIAN - VETERINARY SCIENCE This is a full-time, temporary position to last approximately three (3) months. Salary Range N11: $9.39 per hour POSITION PURPOSE: To prepare histology and immunohistochemistry slides for pathologic examination by faculty and to assist with general laboratory maintenance such as filing microscope slides and blocks, cleaning equipment and loading the tissue processor or automated stainers. KNOWLEDGE, SKILLS AND ABILITIES: Knowledge of: * methods, materials, equipment and techniques of laboratory testing, analysis and media preparation; * basic principles, practices, and procedures of laboratory testing; * laboratory equipment, procedures, operation, and terminology; and * safe laboratory practices; Skill and ability to: * perform basic microtome; * prioritize work; * follow detailed directions and instructions; * use and maintain laboratory equipment; * perform laboratory tests or analysis; * accurately record data onto computer; and * lift up to 50 lbs. COMMENTS: Some work with potentially noxious chemicals is performed in the laboratory under a class II fume hood. Deadline: Open Until Filled Submit SD Job Application Form to: SDSU Human Resources Box 2201 Brookings, SD 57007 (605) 688-4128 ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/a92b2845/attachment.htm From garygill <@t> dcla.com Fri Dec 5 15:16:13 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota St ate University) Message-ID: Doesn't even qualify as salary. Reminds me of the bumper sticker: "My take home pay won't take me home." Gary Gill -----Original Message----- From: Houston, Ronnie [mailto:Ronnie_Houston@bshsi.com] Sent: Friday, December 05, 2003 4:05 PM To: 'SDSU HISTO'; 'histonet@lists.utsouthwestern.edu' Cc: 'fquin@hotmail.com' Subject: RE: [Histonet] Temporary Histology Position Open (South Dakota St ate University) At that salary, the position will stay open for a long, long time Ronnie -----Original Message----- From: SDSU HISTO [mailto:SDSU_HISTO@SDSTATE.EDU] Sent: Friday, December 05, 2003 3:14 PM To: 'histonet@lists.utsouthwestern.edu' Cc: 'fquin@hotmail.com' Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) LABORATORY TECHNICIAN - VETERINARY SCIENCE This is a full-time, temporary position to last approximately three (3) months. Salary Range N11: $9.39 per hour POSITION PURPOSE: To prepare histology and immunohistochemistry slides for pathologic examination by faculty and to assist with general laboratory maintenance such as filing microscope slides and blocks, cleaning equipment and loading the tissue processor or automated stainers. KNOWLEDGE, SKILLS AND ABILITIES: Knowledge of: * methods, materials, equipment and techniques of laboratory testing, analysis and media preparation; * basic principles, practices, and procedures of laboratory testing; * laboratory equipment, procedures, operation, and terminology; and * safe laboratory practices; Skill and ability to: * perform basic microtome; * prioritize work; * follow detailed directions and instructions; * use and maintain laboratory equipment; * perform laboratory tests or analysis; * accurately record data onto computer; and * lift up to 50 lbs. COMMENTS: Some work with potentially noxious chemicals is performed in the laboratory under a class II fume hood. Deadline: Open Until Filled Submit SD Job Application Form to: SDSU Human Resources Box 2201 Brookings, SD 57007 (605) 688-4128 ____________________________________________________________________________ ____________________________________________________ ____________________________________________________________________________ ____________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at 410-442-3250, and permanently delete the original e-mail, attachment(s), and any copies. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031205/d232ba77/attachment.htm From cfranci <@t> rigel.com Fri Dec 5 15:33:02 2003 From: cfranci <@t> rigel.com (Christian Franci) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Message-ID: Can anyone suggest a good, basic but thorough, histology book? Thanks C From nmaclean <@t> ncmir.ucsd.edu Fri Dec 5 15:42:44 2003 From: nmaclean <@t> ncmir.ucsd.edu (Natalie MacLean) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Problems with Vectashield Hard Set Message-ID: <6.0.1.1.1.20031205132933.023dde58@ncmir.ucsd.edu> We have been mounting mouse brain sections perfused with paraformaldehyde using Vectashield Hard Set. It sometimes works beautifully but often, after storing in the dark at 4C as recommended for setting, the samples are ruined. It appears as if they have been dessicated, whether or not this is the case, and the samples are completely unusuable. Is this a unique experience? Does anyone know why this is happening? Are there other recommendations for hard set mounting medias? Thanks so much. Natalie MacLean Department of Neurosciences UCSD From mcauliff <@t> umdnj.edu Fri Dec 5 16:10:34 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) In-Reply-To: References: Message-ID: <3FD1025A.8040804@umdnj.edu> $9.39 an hour for a responsible, skilled postion? It's an insult. AND I'll bet the administration gripes about "how hard it is to get good help these days". Our Housekeeping staff starts at more than that, and they have no training. SDSU HISTO wrote: > LABORATORY TECHNICIAN - VETERINARY SCIENCE > > > > This is a full-time, temporary position to last approximately three > (3) months. > > > > Salary Range N11: $9.39 per hour > > > > POSITION PURPOSE: To prepare histology and immunohistochemistry > slides for pathologic examination by faculty and to assist with > general laboratory maintenance such as filing microscope slides and > blocks, cleaning equipment and loading the tissue processor or > automated stainers. > > > > KNOWLEDGE, SKILLS AND ABILITIES: > > Knowledge of: > > ? methods, materials, equipment and techniques of laboratory > testing, analysis and media preparation; > > ? basic principles, practices, and procedures of laboratory > testing; > > ? laboratory equipment, procedures, operation, and terminology; and > > ? safe laboratory practices; > > > > Skill and ability to: > > ? perform basic microtome; > > ? prioritize work; > > ? follow detailed directions and instructions; > > ? use and maintain laboratory equipment; > > ? perform laboratory tests or analysis; > > ? accurately record data onto computer; and > > ? lift up to 50 lbs. > > > > COMMENTS: Some work with potentially noxious chemicals is performed > in the laboratory under a class II fume hood. > > > > Deadline: Open Until Filled > > > > Submit SD Job Application Form to: SDSU Human > Resources > > > Box 2201 > > > Brookings, SD 57007 > > > (605) 688-4128 > > > > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From ander093 <@t> gold.tc.umn.edu Fri Dec 5 16:23:38 2003 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) In-Reply-To: Message-ID: <5.2.0.9.0.20031205162145.00a23640@ander093.email.umn.edu> This is a joke....right???? If I didn't know better, I'd think it was April 1st. Good luck to you. LuAnn At 02:13 PM 12/5/03 -0600, you wrote: >LABORATORY TECHNICIAN - VETERINARY SCIENCE > > > >This is a full-time, temporary position to last approximately three (3) >months. > > > >Salary Range N11: $9.39 per hour > > > >POSITION PURPOSE: To prepare histology and immunohistochemistry slides >for pathologic examination by faculty and to assist with general >laboratory maintenance such as filing microscope slides and blocks, >cleaning equipment and loading the tissue processor or automated stainers. > > > >KNOWLEDGE, SKILLS AND ABILITIES: > >Knowledge of: > >? methods, materials, equipment and techniques of laboratory >testing, analysis and media preparation; > >? basic principles, practices, and procedures of laboratory testing; > >? laboratory equipment, procedures, operation, and terminology; and > >? safe laboratory practices; > > > >Skill and ability to: > >? perform basic microtome; > >? prioritize work; > >? follow detailed directions and instructions; > >? use and maintain laboratory equipment; > >? perform laboratory tests or analysis; > >? accurately record data onto computer; and > >? lift up to 50 lbs. > > > >COMMENTS: Some work with potentially noxious chemicals is performed in >the laboratory under a class II fume hood. > > > >Deadline: Open Until Filled > > > >Submit SD Job Application Form to: SDSU Human Resources > > >Box 2201 > > >Brookings, SD 57007 > > >(605) 688-4128 > > > > > > From jkiernan <@t> uwo.ca Fri Dec 5 23:04:50 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:18 2005 Subject: Histology books and googling. Was Re: [Histonet] References: Message-ID: <3FD16372.BAA7C610@uwo.ca> Christian Franci wrote: > > Can anyone suggest a good, basic but thorough, histology book? ----------------------------------------- You really should put _something_ in the Subject line! I've supplied a crazy long one for this reply. Histology books. I recommend Wheater's Functional Histology: A Text and Colour Atlas (Book with CD-ROM) by Barbara Young (Editor), John W. Heath (Editor), Churchill Livingstone. US$59.95 from amazon.com Junqueira's Basic Histology: Text & Atlas, 10th Edition (also with a CD) is also very good. Both books have lots of coloured pictures of sections stained with a variety of techniques in addition to haemalum & eosin. Wheater's has the descriptive text closely tied to the illustrations. The text of Junqueira is, perhaps, more thorough. The CDs with both books are rather poorly organized and troublesome to use. Among other things they have unlabelled versions of the illustrations that can be used in teaching lectures. I don't teach histology, so I don't know how valuable such a collection would be. My histology-teaching colleagues have their own collections of photomicrographs of 35mm slides, made from stained sections similar to those used in the lab classes. For a histology course without labs, taught by faculty members deprived of microscope, slides and camera, a CD of good photos may be a necessity. This already is The Shape of Things to Come in medical education. Why fiddle with the condenser and the fine focus when you can use your inkjet printer to make a copy of some expert's best picture? It is a frightening prospect that in the future histology may be taught by people who have not looked at sections with a microscope. Next, a brighter prospect! Googling. Isn't Google wonderful! While typing this reply I googled a two-word query for Wheater's histology, and the book's amazon.com page came up (at the top of a long list) in about 5 seconds, and that's by way of a telephone line modem connection from home. For quite specialized literature searches Google is often faster than PubMed or Web of Science, and it can quickly take you to the full text version of an article. (This is probably because my institution subscribes to the on-line versions of many journals. I doubt if it would work from a hotmail address! Nevertheless, abstracts of all biomedical papers are freely available to all by way of PubMed, which is rapidly and almost intelligently searched by Google.) Possibly some of the other big search engines are as good as Google for finding scientific literature. I've tried 2 or 3 others, and they were useless; worse than they were 2 years ago because clogged up with interuptions by advertisers. Some months ago, a syndicated newspaper (print) columnist expounded in favour of a new verb, "to google," meaning to search the internet. For what it's worth, I support this new verb, and place it along with "to hoover," which concisely means to push a vacuum cleaner over a carpet. Getting a bit off topic, unless there are some histonetters who cut and stain sections of carpets ... Perhaps that's enough. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ________________________________________________ From juan.gutierrez <@t> christushealth.org Sat Dec 6 07:10:08 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Bone Marrow Biopsy Message-ID: Hi Amy! We encountered this problem many years ago. At the time we were using RDO to decalcify our bone marrows, and we found, the hard way, that if you leave them in the acid too long they "loose" their charge. The first thing to do is check your decal times; secondly, if you want to restore some of the charge you can immerse your specimens in a strong base. We tried it and it helped some, but nothing substitutes good time keeping and using fresh solutions. Good luck! Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Amy Self [mailto:ASelf@gmhsc.com] Sent: Fri 12/5/2003 10:38 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Subject: [Histonet] Bone Marrow Biopsy Hello Histonetters ! Hope that everyone had a great Thanksgiving.; I was wandering how all of you netters fixed and decaled your bone marrow biopsy specimens? Here lately the doc that reads out our BM cases says that the biopsy looks kind of smudgy gray. What could this be? He wants to believe that it is not getting deparaffinized enough but all of the other slides in the rack are just fine..... I am open for any suggestions.... Thanks in advance Amy Self Georgetown Memorial Hospital Georgetown, SC 29440 843-527-7179 (work) 843-520-7882 (fax) Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cycling <@t> another.com Sat Dec 6 07:23:18 2003 From: cycling <@t> another.com (cycling@another.com) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] pretreatment for IHC Message-ID: <6419209.1070716998237.JavaMail.root@172.16.100.50> Hi, I think that some sort of protein block in diluents and detergent in the solution are basics, as well as some sort of block for the endogenous enzyme that is your localisation label. After that, then the pretreatment may lead to background, if microwaving etc is too long, or we have been diluting commecial target retrieval solutions to abolish background, and reducing the power level of microwaving. It is variable, some tissues give no bother, whilst others.... And then we can add endogenous biotin as another cause, particular antibody dilutions The variability you have with cell blocks will be a function of the different material, fixation and processing. Dave Edmondson Christie Hospital, Manchester. UK -----Original Message----- >From : ?Scholz, Stephen J.? To : Histonet@Pathology.swmed.edu Date : 05 December 2003 17:32:06 Subject : [Histonet] pretreatment for IHC Hello Histonet, > >I am having a problem with background staining on IHC slides. This is only happening in cell block specimens. Is this common on cell blocks? Should I do a pretreatment on these? What pretreatments do others prefer? Should I peroxide quench? Before or after? What about blockers? Do I ramble on and ask to many questions? > >Thanks for all your help, > >Stephen J. Scholz HT(ASCP) >Histology Coordinator >OSF St. Anthony Medical Center >Rockford IL > >Phone: 815-395-5410 >Fax: 815-395-5364 >e-mail: sjscholz@osfhealthcare.org > > > -- Personalised email by http://another.com From cycling <@t> another.com Sat Dec 6 07:35:38 2003 From: cycling <@t> another.com (cycling@another.com) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Bone Marrow Biopsy Message-ID: <2871971.1070717738864.JavaMail.root@172.16.100.50> Hi Amy I was wondering whether you may have features of "blue-grey" artefact in your biosies. If I understand the theory on this, then it is perhaps down to clearing agent being around in the early stages of processing. Shandon /ThermoShandon were recommending a warm water wash between processing runs to wash out the pipes on the machines. There was other advice to make sure that fixation was not compromised, that's to say not too short. We have been using Neutral buffered formalin, isotonic for saline followed by decal in EDTA. It is a long time now but I still think the chromatin not quite so condensed as with the old Formic acid decal Dave Ed' Christie Hospital Manchester UK -----Original Message----- >From : Amy Self To : ?'histonet@lists.utsouthwestern.edu'? Date : 05 December 2003 16:38:47 Subject : [Histonet] Bone Marrow Biopsy > > Hello Histonetters ! Hope that everyone had a great >Thanksgiving.; > > I was wandering how all of you netters fixed and decaled your bone >marrow biopsy specimens? Here lately the doc that reads out our BM cases >says that the biopsy looks kind of smudgy gray. What could this be? He >wants to believe that it is not getting deparaffinized enough but all of the >other slides in the rack are just fine..... I am open for any >suggestions.... > >Thanks in advance > > Amy Self > Georgetown Memorial Hospital > Georgetown, SC 29440 > > 843-527-7179 (work) > 843-520-7882 (fax) > > > > > > >Note: The information contained in this message may be privileged and >confidential and protected from disclosure. If the reader of this message is >not the intended recipient, or an employee or agent responsible for >delivering this message to the intended recipient, you are hereby notified >that any dissemination, distribution or copying of this communication is >strictly prohibited. If you have received this communication in error, >please notify us immediately by replying to the message and deleting it from >your computer. Thank you. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Personalised email by http://another.com From MinHan.Tan <@t> vai.org Sat Dec 6 08:19:13 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] pretreatment for IHC Message-ID: <74D0F0AB07F2E647A02D839ED79520F94A9DDC@VAIEXCH02.vai.org> I think that the appropriate thing to do for increased background, rather than attempt all kinds of methods, is to elicit the step at which problems are occurring by use of appropriate controls. This will save you both time and money ultimately. You can run all these in a single experiment - just make sure that the slides are cut from the same specimen, which is known to stain positive. There are quite a few causes of increased background, of course - but this will allow you to find out with the minimum of fuss, and within one day. Slide with substrate alone Slide with substrate + ABC Slide with substrate + ABC + secondary antibody Slide with substrate + ABC + primary antibody + secondary antibody Slide with substrate + ABC + primary antibody + secondary antibody + peroxidase quenching Slide with substrate + ABC + primary antibody + secondary antibody + blocking reageants Slide with substrate + ABC + primary antibody + secondary antibody + peroxidase quench + blocking reageants. (dissimilar species serum) Min-Han Tan Van Andel Research Institute -----Original Message----- From: Scholz, Stephen J. [mailto:Stephen.J.Scholz@osfhealthcare.org] Sent: Friday, December 05, 2003 12:32 PM To: Histonet@Pathology.swmed.edu Subject: [Histonet] pretreatment for IHC Hello Histonet, I am having a problem with background staining on IHC slides. This is only happening in cell block specimens. Is this common on cell blocks? Should I do a pretreatment on these? What pretreatments do others prefer? Should I peroxide quench? Before or after? What about blockers? Do I ramble on and ask to many questions? Thanks for all your help, Stephen J. Scholz HT(ASCP) Histology Coordinator OSF St. Anthony Medical Center Rockford IL Phone: 815-395-5410 Fax: 815-395-5364 e-mail: sjscholz@osfhealthcare.org This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031206/e83056f9/attachment.htm From asmith <@t> mail.barry.edu Sat Dec 6 10:23:47 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Message-ID: <494304423C63E246A5CF87A3AEEB577011EDAC@bumail01.barrynet.barry.edu> Wheater's Functional Histology is a good short book; it covers the basics. Ross, Kaye, and Pawlina's Histology: A Text and Atlas is a good long book; it covers a lot of detail well in 800 pages. -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Christian Franci Sent: Friday, December 05, 2003 4:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Can anyone suggest a good, basic but thorough, histology book? Thanks C _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From rschoon <@t> email.unc.edu Sat Dec 6 12:57:51 2003 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) References: <3FD1025A.8040804@umdnj.edu> Message-ID: <3FD226AF.1050304@email.unc.edu> This must be some kind of joke.. My teenage son makes more at the local mall. Robert Schoonhoven Geoff McAuliffe wrote: > $9.39 an hour for a responsible, skilled postion? It's an insult. AND > I'll bet the administration gripes about "how hard it is to get good > help these days". Our Housekeeping staff starts at more than that, and > they have no training. > > SDSU HISTO wrote: > >> LABORATORY TECHNICIAN - VETERINARY SCIENCE >> >> >> >> This is a full-time, temporary position to last approximately three >> (3) months. >> >> >> >> Salary Range N11: $9.39 per hour >> >> >> >> POSITION PURPOSE: To prepare histology and immunohistochemistry >> slides for pathologic examination by faculty and to assist with >> general laboratory maintenance such as filing microscope slides and >> blocks, cleaning equipment and loading the tissue processor or >> automated stainers. >> >> >> KNOWLEDGE, SKILLS AND ABILITIES: >> >> Knowledge of: >> >> ? methods, materials, equipment and techniques of laboratory >> testing, analysis and media preparation; >> >> ? basic principles, practices, and procedures of laboratory >> testing; >> >> ? laboratory equipment, procedures, operation, and >> terminology; and >> >> ? safe laboratory practices; >> >> >> >> Skill and ability to: >> >> ? perform basic microtome; >> >> ? prioritize work; >> >> ? follow detailed directions and instructions; >> >> ? use and maintain laboratory equipment; >> >> ? perform laboratory tests or analysis; >> >> ? accurately record data onto computer; and >> >> ? lift up to 50 lbs. >> >> >> >> COMMENTS: Some work with potentially noxious chemicals is performed >> in the laboratory under a class II fume hood. >> >> >> Deadline: Open Until Filled >> >> >> >> Submit SD Job Application Form to: SDSU Human >> Resources >> >> >> Box 2201 >> >> >> Brookings, SD 57007 >> >> >> (605) 688-4128 >> >> >> >> >> >> >> > From fmonson <@t> wcupa.edu Sat Dec 6 13:14:30 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Good Histology Books-One OpinionOnly Message-ID: Here are my recommendations, if you can find copies. Bloom and Fawcett a Textbook of Histology (at Amazon.com, used, many) by Don W. Fawcett (Editor), et al * Hardcover: 964 pages ; Dimensions (in inches): 1.75 x 10.50 x 7.50 * Publisher: Lippincott Williams & Wilkins Publishers; 12th edition (December 1994) * ASIN: 0412046911 --------------------------------------------------------------------- Ham's Histology by Cormack Hardcover * Publisher: Lippincott Williams & Wilkins Publishers; 9th edition (April 1987) * ASIN: 0397506813 -------------------------------------------------- Histology for Pathologists by Stephen S., Md. Sternberg (Editor), Stemberg Hardcover: 1216 pages ; Dimensions (in inches): 2.50 x 11.50 x 9.25 Publisher: Lippincott Williams & Wilkins Publishers; 2nd edition (December 1997) ISBN: 0397517181 | Prof. Fawcett's text is a decade old, and DOES NOT digress much from light microscopic and fine structure of the tissues and cells that comprise them. The almost 1,000 pages is about right, in my opinion, for a text that covers structure well. What follows is a philosophical discussion of why I chose the text listed above. If only the basics of histology are desired, then, my recommendation is a hard cover copy of this text. Ham & Cormack (I have the 8th edition) is the best of the non-medical functional histology books published in the 1960's-1980's. These texts focused on the functional integration of the subject of histology. The text listed above I have NOT seen, so it is listed only to provide a means to search for old copies of the 8th ed., if that is desirable. I have kept the Ham and Cormack, because in it I learned first particulars about immunology and second about the various means of determining, by treatment regime, the structure of articular cartilage after healing from injury. Dr. Sternberg's book is 4x the price of the Fawcett tome. It is, as its title describes, an histology book FOR pathologists. Whether surprising or not, pathologists are, though often more specialized that I would prefer for myself, the most complete histologists, though their concerns are limited by and to their clinical foci. This is a book that, in its second edition, has changed from the first, by loss and addition of chapters. Thus, I have both on my shelf, and I will likely not divest myself of either. Interestingly, and not unexpectedly, the concentration in this text is NOT on the basics, but rather on the nuances of 'normal' cells and tissues in the context of the clinical milieu, as determined by Dr. Sternberg, the editor. Distinct from Fawcett are chapters on 'Apoptosis', the "Myofibroblast', 'Paraganglia', and 'Neuroendocrine System'. Disclaimer! Given my experience, my feelings are colored both by what I already know and what I would consider educationally viable. Thus, all that I have said, and will say, is slanted by those factors. The result is that I shy away from paperback texts, because any text I purchase I will wish to keep and use. Finally, over the years, I have learned a perhaps unhappy/unreasonable aversion to 500 page paperback books in anatomy, except those whose purpose is to provide recreational time with colored pencils. These 'anticlivities' prevent me from painting with a broad, unbiased brush, and thus, I am not, by failing to include those texts mentioned by others, making either derogatory or dismissive allusions to them. What follows my first good-bye as a P.S. is mere preaching, which should be avoided by those who either lack interest in such ramblings or don't believe the 'WHY' of the opinions of the old, infirm, and soon-to-be irrelevant or gone old(er) folks. If you continue, remember that you have been warned! Cheers, FCM (for those who have the discipline to quit here) P.S. It is impossible to recommend textbooks to individuals in the absence of knowledge about the goal(s) of the person submitting the query. However, if I am asked about texts, I try to explain my choices. For the above three books, which I would recommend to a graduate student about to study histology, the first for its wonderful micrographs (except for the overly red light micrographs) and residual Maximow ink drawings, the second, for its focus on structure and function, and the third for its attention, by specialists, to the newest information on the structure and function of tissues and their cellular and extracellular components. First, I have always believed that a text that one purchases for 'the course' and then discards was NEVER worth the 'energy of acquisition'. I still have many of the text books I owned as a graduate student, because they serve as useful reminders of the content I was supposed to retain. There are also those purchases I have made after long (often decades) of searching. One of the best reasons to save a text has already been given. Another is the purely scholarly reason - the bibliographies. It may sound silly, but one of the first parts of planning a course is a definition of the philosophcal focus of the subject to be presented. With respect to histology, there are two approaches. One defines the limit of the course as 'knowledge of' the stripped-down fundamentals of the subject. Such a course is confined to the basic tissues and their cellular and morphological characteristics, and, in the lab, with the minimum essentials of identification. Another defines the limit of the course as 'knowledge of' structure as additionally characterized by function. That is, "Functional Histology". This course cannot be taught to sophomores. To learn it properly, one should have some exposure to physiology, embryology, and comparative anatomy. Where the former represents courses that rest on the foundation of "...The thigh bone's connected to the hip bone...", the latter attends to the business of connecting the structure of tissues to the real 'business' of tissues. Where the first teaches the basics of tissue organization, the latter two attempt to integrate structure with function. That to me has always been the goal. [Learning to spell a word while not being required to learn its definition and its common, or uncommon applications, is a useless waste of time and energy. Intussusception and diapedesis are words that, for us in biology, relate to embryology and immunology; yet to know only how to spell them would waste valuable cognitive 'bandwidth'. These terms I learned from my study of anatomy, and they are almost as much fun as transcription, osculation, translation, and replication (terms all related to human reproduction! and all used in the one multiple choice exam I was once forced to give to my students!).] The last point to be made is about "Comparative" biology. This part of the subject of histology may be confined to vertebrates, to vertebrates and invertebrates, but existed, when I was a student as a viable source of textbooks for vertebrate histology, even though they were abbreviated in one manner or other. Comparative histology is where one learns about 'teeth' in the skin of a shark (and their/its use as sandpaper) and 'breathing' (gas exchange) thru the skin of a frog (but not a toad!), kidneys with ONE glomerulus, and acellular bone. Similarly, comparative biochemistry is where one learns about fish that lack red blood cells but have free hemoglobin in the blood and excellent survival rates, comparative physiology where salmon demonstrate significant osmoregulatory flexibility/adaptability when they first go to sea and then return to spawn. Finally, from a comparison of LDH in human/mammalian embryos and neonates, one learns about biochmeical adaptation on the fly at/around parturition. Indeed from all of this, one learns to evaluate structure within a VERY wide window on living systems. An education that is properly organized much more preferable than one that is strongly focused, except at the end, when all that came before can be used to study and understand the very, very specific. H.F.E. Cheers to all, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging West Chester University of Pennsylvania West Chester, PA, 19383 From CrochiereSteve <@t> aol.com Sat Dec 6 20:19:22 2003 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Pathologist's Assistant Assistant? Message-ID: <30.4ba9981a.2d03e82a@aol.com> I find that the PA's feel they are above the rest of the lab and feel they shouldn't need to write down numbers or get their own paper towels etc... It's quite a waste to pay someone $19-22/hour just to kiss some elitist's ass. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031206/40eaea97/attachment.htm From jkiernan <@t> uwo.ca Sat Dec 6 23:37:57 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Pathologist's Assistant Assistant? References: <30.4ba9981a.2d03e82a@aol.com> Message-ID: <3FD2BCB5.886C02BA@uwo.ca> Dear CrochiereSteve@aol.com, whoever you are in real life, Having written and posted your piece (quoted below), please will you explain what an "elitist" is and why he, she or it requires kisses on the arse. (Arse is the old word for bum; an ass is a donkey. I have sat on one, and it walked when prodded. Intimate oral contact probably has no effect on the arse of an ass.) ________________ CrochiereSteve@aol.com wrote: > > I find that the PA's feel they are above the rest of > the lab and feel they shouldn't need to write down > numbers or get their own paper towels etc... > It's quite a waste to pay someone $19-22/hour just to > kiss some elitist's ass. From lpwenk <@t> covad.net Sun Dec 7 06:41:18 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Stain for H.Pylori References: <20031203165805.14533.qmail@web40905.mail.yahoo.com> Message-ID: <003301c3bcbf$6b689fa0$8732fea9@hppav> Alcian yellow is not being made anymore, anywhere. ANATECH, Ltd. has a substitute, called HP Yellow, if I remember correctly. The usual TB/AY staining procedure has to be modified slightly, in order for this yellow dye to bind, but according to the procedure I read, it's just one extra solution. 1-800-ANATECH. (No, I don't work for them. Just passing the information along.) We don't do the Tol. Bl/Al Y stain. We do the Diff-Qwik - the same one that is done in cytology. It's fast, reusable (we use it about 1 week, then throw it out, but then we're staining 20-30 slides/day). The directions say to run down to abs. reagent alcohol, then go into the DQ fixative (methanol). If you run your slides down to water, it's OK. Just put them directly into the DQ fix before staining them. I have tried to go from abs. directly into the stain, skipping the methanol. (I figured, alcohol is alcohol.) Well, it doesn't stain right if you skip the methanol fixative. Don't know why. Just thought I'd pass this on. Our procedure is below. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI MICRO-ORGANISM - DIFF-QUIK PREPARED BY: Peggy A. Wenk, BS, HTL(ASCP)SLS ADOPTED BY: _______________________________ DATE: DATE INITIAL DATE INITIAL DATE INITIAL DATE INITIAL REVIEWED REVIEWED REVIEWED REVIEWED SUPERSEDES: PURPOSE: This stain demonstrates Helicobacter pylori, which is present in many gastric biopsy specimens with active gastritis. It can also be used to non-differentially stain bacteria and for the demonstration of some parasites. PRINCIPLE: Diff-Quik is a modified Giemsa/ Romanowsky stain. Diff-Quik solution I contains eosin, which gives a pink color to cytoplasm. Diff-Quik solution II contains Azure A and Methylene Blue. The methylene blue is an impure dye, and will oxidize into azure A, azure B, and methylene violet, thereby giving a wider range of colors. Both solutions contain buffers, which establish the best pH for staining. FIXATION: Formalin fixed tissues preferred. Hollandes fixed tissues may stain false negative, due to the Hollandes solution creating holes in the walls of the bacteria. Steiner and Steiner is recommended for Hollandes-fixed tissues. TECHNIQUE: Cut routine paraffin sections at 5 um. CONTROL: Gastric tissue with H. pylori preferred. Any tissue with small, gram negative bacteria may also be used, such as E. coli. QUALITY CONTROL: 1. Do not use this stain with Hollandes fixed tissues. Steiner and Steiner will demonstrate these. 2. Use a microscope to determine the assess quality of staining. EQUIPMENT: Erlenmeyer flask, graduated cylinders, forceps. CAUTION: FOLLOW STANDARD SAFETY PROCEDURES WHEN PREPARING STAINS. DIFF-QUIK Fixative contains methanol which is poisonous. May be fatal or cause blindness if swallowed. Cannot be made nonpoisonous. Liquid and vapor are flammable. Harmful if inhaled or absorbed through skin. May cause skin and eye irritation. DIFF-QUIK Solution I - Contains Eosin, Buffer and 0.001% sodium azide. DIFF-QUIK Solution II - Contains Azure A, Methylene Blue, and a buffer. ACETIC ACID is an acid. Add drop by drop to solution. May cause skin/eye burns. REAGENTS: DIFF-QUIK FIXATIVE DIFF-QUIK SOLUTION I DIFF-QUIK SOLUTION II (Baxter catalog #B4132-12) ACID-ALCOHOL SOLUTION 95% alcohol 368.0 mL Distilled water 132.0 mL Acetic acid (HCOOH) 1.25 mL Mix together alcohol and water. Slowly add, drop by drop, acetic acid to solution. Store at room temperature in a plastic or glass container. Good for several months. PROCEDURE - Diff-Quik: 1. Deparaffinize and place in several changes of absolute alcohol. NOTE: If possible, proceed to Step 2 after the absolute alcohol. However, if slides have already been run in water, go to Step 2 and extend the time in Step 2 to 2 minutes. NOTE: If a frozen section or cytology specimen, follow directions under "PROCEDURAL NOTES." 2. Place slides directly into Diff-Quik Fixative Solution 1 minute 3. Place slides directly into Diff-Quik Solution I 2 minutes 4. Place slides directly into Diff-Quik Solution II 4 minutes 5. Rinse quickly in 2-3 changes of distilled water 1-2 seconds each 6. Pour on, pour off quickly Acid-Alcohol Solution 1-2 seconds 7. Rinse in distilled water, 2-4 changes 5 seconds each 8. Allow slides to air dry completely 2 minutes or longer 9. Coverslip with synthetic mounting media RESULTS: Bacteria, including Helicobacter pylori deep-blue Fungus deep-blue Nuclei blue Cytoplasm, collagen, muscle, cytoplasm varying shades blue and pink PROCEDURAL NOTES: 1. Check slides after water rinse (Step #6). Slides may be restained, if necessary. 2. If tissues have been fixed in Hollandes, use the Steiner and Steiner procedure, as the bacteria may be false negative is stained with the Diff-Quick. 3. Slides may be left in Diff-Quik Fixative solution (blue) longer than 1 minute, without harm to the tissue. 4. IF SPECIMEN IS A FROZEN SECTION: Use the following procedure: Do NOT deparaffinize and dehydrate (Step #1) Place in Diff-Quik Fixative solution (blue) 1-5 minutes Go directly to Step 2. 5. IF SPECIMEN IS A CYTOLOGY SPECIMEN: Use the following procedure: Deparaffinize and hydrate through absolute alcohol. Place in Diff-Quik Fixative solution (blue) 1 minute Go directly to Step 2. 6. If specimen must go through the Diff-Quik Fixative solution, tissue may be left in solution longer than 1 minute, without harm to the tissue. REFERENCES: Manufacturer's notes that come with Diff-Quik Solutions Ray Skipper and Don B. DeStephano: A Rapid Stain for Campylbacter pylori in gastrointestinal sections using Diff-Quik. J. Histotechnol 12:303-304, Dec. 1989 ----- Original Message ----- From: Jill Cox To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 03, 2003 11:58 AM Subject: [Histonet] Stain for H.Pylori Hello everyone, Has anyone done a Toluidine blue/Alcian yellow stain for H. Pylori? If so how do the Doctors like it? Or, what other stains are you doing for that. I am trying to get away from the Genta!! Thanks in advance Jill Cox HT (ASCP) Histology Supervisor Seattle Histology Lab ------------------------------------------------------------------------------ Do you Yahoo!? Free Pop-Up Blocker - Get it now -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031207/c69b23d7/attachment.htm From stancelb <@t> msn.com Sun Dec 7 07:45:22 2003 From: stancelb <@t> msn.com (Barbara Stancel) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histo Position at SDSU-my comments Message-ID: Dear SDSU Histo, I feel so for you and your plight to obtain pretrained histology help for a veterinary histology position, temporary or full time. Working in a university setting can be tough. Pay is crappy. Days can be long. And even though the description may say "histology position" they usually tack on " and other duties as assigned" which may include necropy duty and in some research areas, the heart wrenching job of helping with euthanasia. The perks may include good health/dental insurance, set hours, no weekend work, an avenue of new experiences, and possibly the opportunity to work with graduate students and veterinary professors. Some of them are wonderful and others wear their multitude of degrees as body armour. It provides them protection from the backlash of punishments of poor or misguided decisions. For the most part my experiences (in the trenches of the lowest paid position in the Pathology/Parasitology department in a university setting) were positive. I was fortunate to work with researchers who appreciated my work and were not above asking for my input on research projects. But in the 1970's and 80's, the general montra was "The pay is bad, but we can't do anything about that. Besides, we can always find a student's spouse who will be glad to get the money." And usually they were right. I agree with all those who have condemned the pay per hour. It is bad. But when the personnel and admistration say that is all they will pay......that's it. It was embarrassing to advertise my low paying positions, but what choices did I have. It can be disheartening to send a temp job notice to the HISTONET and then get "blasted" for advertising a pay scale you have no control over, but desparately need the help. Hopefully you can print out the many negative responses for your supervisor. Maybe even talk to local establishments and find out their pay scales for employees. Comparing to the local hospitals may not be beneficial since most vet universities refuse to be compared to human workers. Or how about contacting some of the temp agencies for lab personnel. See how much it would cost to hire a temp through an agency. Take this information to your supervisor or personnel department. I will be retiring in a few years. I will probably continue to stay involved in some way with lab work. At that time, I may even be tempted to travel and take temporary positions for the sake of seeing different areas of the country.......but not South Dakota in the winter!!!! Best of Luck, Histologically yours, Barbara in Athens, Georgia (Where our coldest days are in the 20's. Snow-days are may count in twos and threes per year, AND they call off schools and work!! I can't drive in it. I don't own snow tires. Tire chains tear up our roads. And I want to stay at home a day and enjoy the beauty of snow, even when it comes with ice and sleet and downed power lines.) ________________________________________________________ LABORATORY TECHNICIAN - VETERINARY SCIENCE - This is a full-time, temporary position to last approximately three (3) months. Salary Range N11: $9.39 per hour POSITION PURPOSE: To prepare histology and immunohistochemistry slides for pathologic examination by faculty and to assist with general laboratory maintenance such as filing microscope slides and blocks, cleaning equipment and loading the tissue processor or automated stainers. KNOWLEDGE, SKILLS AND ABILITIES: Knowledge of methods, materials, equipment and techniques of laboratory testing, analysis and media preparation; basic principles, practices, and procedures of laboratory testing laboratory equipment, procedures, operation, and terminology; and safe laboratory practices; Skill and ability to perform basic microtome; prioritize work; follow detailed directions and nstructions; use and maintain laboratory equipment; perform laboratory tests or analysis;accurately record data onto computer; and lift up to 50 lbs. _________________________________________________________________ Cell phone ‘switch’ rules are taking effect — find out more here. http://special.msn.com/msnbc/consumeradvocate.armx From mprice26 <@t> juno.com Sun Dec 7 10:11:08 2003 From: mprice26 <@t> juno.com (Marsha R Price) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) Message-ID: <20031207.101109.3824.3.mprice26@juno.com> Surely it is a joke. They must not be trying to hire a registered Histotech. Marsha Price On Sat, 06 Dec 2003 13:57:51 -0500 Robert Schoonhoven writes: > This must be some kind of joke.. My teenage son makes more at the > local mall. > > Robert Schoonhoven > > Geoff McAuliffe wrote: > > > $9.39 an hour for a responsible, skilled postion? It's an insult. > AND > > I'll bet the administration gripes about "how hard it is to get > good > > help these days". Our Housekeeping staff starts at more than that, > and > > they have no training. > > > > SDSU HISTO wrote: > > > >> LABORATORY TECHNICIAN - VETERINARY SCIENCE > >> > >> > >> > >> This is a full-time, temporary position to last approximately > three > >> (3) months. > >> > >> > >> > >> Salary Range N11: $9.39 per hour > >> > >> > >> > >> POSITION PURPOSE: To prepare histology and immunohistochemistry > > >> slides for pathologic examination by faculty and to assist with > >> general laboratory maintenance such as filing microscope slides > and > >> blocks, cleaning equipment and loading the tissue processor or > >> automated stainers. > >> > >> > >> KNOWLEDGE, SKILLS AND ABILITIES: > >> > >> Knowledge of: > >> > >> ? methods, materials, equipment and techniques of > laboratory > >> testing, analysis and media preparation; > >> > >> ? basic principles, practices, and procedures of > laboratory > >> testing; > >> > >> ? laboratory equipment, procedures, operation, and > >> terminology; and > >> > >> ? safe laboratory practices; > >> > >> > >> > >> Skill and ability to: > >> > >> ? perform basic microtome; > >> > >> ? prioritize work; > >> > >> ? follow detailed directions and instructions; > >> > >> ? use and maintain laboratory equipment; > >> > >> ? perform laboratory tests or analysis; > >> > >> ? accurately record data onto computer; and > >> > >> ? lift up to 50 lbs. > >> > >> > >> > >> COMMENTS: Some work with potentially noxious chemicals is > performed > >> in the laboratory under a class II fume hood. > >> > >> > >> Deadline: Open Until Filled > >> > >> > >> > >> Submit SD Job Application Form to: SDSU > Human > >> Resources > >> > >> > > >> Box 2201 > >> > >> > > >> Brookings, SD 57007 > >> > >> > > >> (605) 688-4128 > >> > >> > >> > >> > >> > >> > >> > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From RSRICHMOND <@t> aol.com Sun Dec 7 12:27:58 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Re: Pathologist's Assistant Assistant? Message-ID: <26.427bfbca.2d04cb2e@aol.com> Steve Crochiere sez >>I find that the PA's feel they are above the rest of the lab and feel they shouldn't need to write down numbers or get their own paper towels etc... It's quite a waste to pay someone $19-22/hour just to kiss some elitist's ass.<< Indeed, flagrant presumption - only pathologists are allowed such attitudes! ;-) (John Kiernan - now there's a man who knows his arsis from his thesis....) Bob Richmond Samurai Pathologist Knoxville TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031207/5f1ba033/attachment.htm From RSRICHMOND <@t> aol.com Sun Dec 7 12:33:02 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:18 2005 Subject: [Histonet] Re: Stain for H.Pylori Message-ID: <1e6.14e8e8c4.2d04cc5e@aol.com> Lee and Peggy Wenk offer a Diff-Quik technique for Helicobacter pylori. My personal preference - supported by at least some of the literature - is to omit the Diff-Quik I (eosin). - I've seen most of the stains in current use, in the various labs I work in. I think that IHC makes it easier to work without oil immersion. My current practice with Diff-Quik II is to examine all positives and many of the apparent negatives with a 100x oil immersion lens. I think this really makes a difference, particularly if your high-dry (45x) objective is less than perfect. Diff-Quik is of course a trade name. My personal experience is that generics work quite as well. Bob Richmond Samurai Pathologist Knoxville TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031207/936b7e82/attachment.htm From phyllitis <@t> hotmail.com Sun Dec 7 13:05:56 2003 From: phyllitis <@t> hotmail.com (MK) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] unsubscribe References: Message-ID: ----- Original Message ----- From: "HistoNet Server" To: "* *" Sent: Thursday, June 05, 2003 2:05 PM Subject: re: subscribe > Your address has been added to the addresses that comprise this Listserv > List. > Welcome to HISTONET. This is an electronic mailing list for the exchange of > information pertaining to histotechnology and related fields. > > PLEASE SAVE THIS MESSAGE. > It contains useful information about how to use the list and what to do if you > experience problems. It also includes some basic rules for email etiquette > (Netiquette) which will be helpful to those who are new to this form of > communication. > > WHAT IS A LISTSERVER? > A list server is a computer that runs software which will receive incoming > electronic mail (email) messages and reroute them automatically to everyone on > the subscriber list. Email uses the vast expanse of the Internet to allow > almost instantaneous communication between networked computers around the > world. Our system uses the LISTSTAR software from Quarterdeck Corporation > (California) and can currently send about 30 messages a minute. With the > present number of subscribers, we are processing about 10,000 outbound > messages a day. > > WHO SHOULD SUBSCRIBE? > Anyone interested in research or clinical applications of histology, > immunohistochemistry, in-situ hybridization pathology, and electron microscopy > may find Histonet informative and useful. Currently, there are more than 850 > subscribers from all over the world. Subscribers include hospital employees > from major urban centers and obscure remote locales, university researchers, > botanists and the employees of commercial laboratories, government agencies, > veterinary facilities and a wide variety of commercial industrial ventures. > > WHO RUNS HISTONET? > The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using > hardware and software owned by the University of Texas Southwestern Medical > School, Department of Pathology in Dallas, Texas. If you have any questions or > problems with Histonet please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us. > > HOW DOES THE LIST WORK? > This server, unlike many systems, uses ONLY ONE ADDRESS to send commands to > the computer and to post messages. The server will recognize commands sent in > the SUBJECT line of the message and only when they are spelled exactly as > listed below. Anything not identified as a command will be circulated to > EVERYONE on the list. > > The following is a list of commands the server recognizes: > > subscribe > Your address will be added to the list of subscribers. You will then be able > to send messages to this list that will be forwarded to all other list > subscribers. You will begin to receive all messages sent to the list by other > subscribers. > > subscribe digest > Your address will be added to the list of subscribers who receive a digest > instead of each forwarded message. A digest is a compilation of all the > messages received in a 24 hour period. It is sent to the digest subscribers > every night after midnight. Digest subscribers can post and respond to > messages the same as "real-time" subscribers. > > digests > A list of available digests will be returned to you. Histonet stores old > messages as daily digests for approximately three months. To read previous > messages, copy the list of available digests, mark the dates of interest and > return it to the server. > > unsubscribe > Your address will be removed from the list of subscribers. > You will no longer be able to send messages to the members > of the list. > > help > A list of the commands recognized by the server will be returned to you. > > WHAT ARE THE RULES? > You may post any questions you wish pertaining to histology, pathology, > in-situ hybridization, immunohistochemistry etc. Equipment and reagent > evaluations, laboratory management issues, government regulations, and job > opportunities are all appropriate topics. The University asks that we restrict > the use of its hardware and software to business purposes only (occasional > jokes do slip through but PLEASE use restraint). Vendors and those with > commercial interests in histology products are welcome contributors however, > we ask that blatant advertisements be avoided at all times. It is fine to > refer to product that your company produces if it is pertinent to a topic > being discussed on the list. Unsolicited advertisements are poorly tolerated > by the members and you will likely receive a number of negative comments if > you overstep the boundaries. Please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us if you are not sure about the appropriateness > if a message you wish to post. > > > BASIC HISTONET "NETIQUETTE" > It is most helpful to the list members if you post your responses to queries > to everyone on the list and not just as a personal reply to the person asking > the question. That way duplicate messages are minimized and we all learn from > each other's comments. > > Likewise, if you post a question and get a number of responses back directly > to you, it is helpful to everyone if you could send out a summary of the > replies you got to Histonet. > > Please avoid abbreviations unless they are explained in your message. For > example: immunohistochemistry (IHC). This list circulates to a wide variety of > individuals and what seems obvious to you may have no meaning on the other > side of the world. > > Please sign your letter and include your institution or affiliation and > location. Not all email systems have headers which identify the sender. > > Do use the subject line to indicate the topic of your message. > > DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. > > Please send questions and problems about the list directly to Linda Margraf at > LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 > subscribers on the list. Be careful when sending commands to the server to put > the command in the SUBJECT LINE and spell it correctly. > > Please do not send images as attachments with your message. We can now post > images at our web site (http://pathcuri1.swmed.edu). To have an image posted > send it to Herb Hagler at herb.hagler@email.swmed.edu. > > > > > es > > From tigersnake <@t> ecybermind.net Sun Dec 7 17:44:00 2003 From: tigersnake <@t> ecybermind.net (Paul Howard Lockwood) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) Message-ID: <200312072040.hB7Kemx06563@ginsberg.ecybermind.net> To All, Having read the ad for the temp position, I have to agree that it is an insult. They must be trying to get someone very desperate for a job, or they hope to hire someone just graduated from college, or a tech school, who doesn't know any better. Just thought I'd add my two copper coins worth. Paul Lockwood 12/7/03 8:11:08 AM, Marsha R Price wrote: >Surely it is a joke. They must not be trying to hire a registered >Histotech. >Marsha Price > >On Sat, 06 Dec 2003 13:57:51 -0500 Robert Schoonhoven > writes: >> This must be some kind of joke.. My teenage son makes more at the >> local mall. >> >> Robert Schoonhoven >> >> Geoff McAuliffe wrote: >> >> > $9.39 an hour for a responsible, skilled postion? It's an insult. >> AND >> > I'll bet the administration gripes about "how hard it is to get >> good >> > help these days". Our Housekeeping staff starts at more than that, >> and >> > they have no training. >> > >> > SDSU HISTO wrote: >> > >> >> LABORATORY TECHNICIAN - VETERINARY SCIENCE >> >> >> >> >> >> >> >> This is a full-time, temporary position to last approximately >> three >> >> (3) months. >> >> >> >> >> >> >> >> Salary Range N11: $9.39 per hour >> >> >> >> >> >> >> >> POSITION PURPOSE: To prepare histology and immunohistochemistry >> >> >> slides for pathologic examination by faculty and to assist with >> >> general laboratory maintenance such as filing microscope slides >> and >> >> blocks, cleaning equipment and loading the tissue processor or >> >> automated stainers. >> >> >> >> >> >> KNOWLEDGE, SKILLS AND ABILITIES: >> >> >> >> Knowledge of: >> >> >> >> ? methods, materials, equipment and techniques of >> laboratory >> >> testing, analysis and media preparation; >> >> >> >> ? basic principles, practices, and procedures of >> laboratory >> >> testing; >> >> >> >> ? laboratory equipment, procedures, operation, and >> >> terminology; and >> >> >> >> ? safe laboratory practices; >> >> >> >> >> >> >> >> Skill and ability to: >> >> >> >> ? perform basic microtome; >> >> >> >> ? prioritize work; >> >> >> >> ? follow detailed directions and instructions; >> >> >> >> ? use and maintain laboratory equipment; >> >> >> >> ? perform laboratory tests or analysis; >> >> >> >> ? accurately record data onto computer; and >> >> >> >> ? lift up to 50 lbs. >> >> >> >> >> >> >> >> COMMENTS: Some work with potentially noxious chemicals is >> performed >> >> in the laboratory under a class II fume hood. >> >> >> >> >> >> Deadline: Open Until Filled >> >> >> >> >> >> >> >> Submit SD Job Application Form to: SDSU >> Human >> >> Resources >> >> >> >> >> >> >> Box 2201 >> >> >> >> >> >> >> Brookings, SD 57007 >> >> >> >> >> >> >> (605) 688-4128 >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> > >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >________________________________________________________________ >The best thing to hit the internet in years - Juno SpeedBand! >Surf the web up to FIVE TIMES FASTER! >Only $14.95/ month - visit www.juno.com to sign up today! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Megan.Kear <@t> hunter.health.nsw.gov.au Sun Dec 7 19:12:33 2003 From: Megan.Kear <@t> hunter.health.nsw.gov.au (Megan Kear) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] collagen Message-ID: Hi , could someone help with a recommendation for staining "newly laid colagen",we think it is type v but not quite sure.Any suggestions would be of great help. Thank You Megan Kear HAPS Newcastle Australia From m_masri80 <@t> yahoo.com Sun Dec 7 19:11:31 2003 From: m_masri80 <@t> yahoo.com (mashita masri) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] unsubscribe Message-ID: <20031208011131.77484.qmail@web41209.mail.yahoo.com> --------------------------------- Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031207/49b504da/attachment.htm From histo_tek <@t> msn.com Sun Dec 7 21:10:03 2003 From: histo_tek <@t> msn.com (Pamela Wirth) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] HTL and Master's degree Message-ID: Have a review coming up this week, just wondering how many HTL's out there also have an MS degree, also with or without QIHC (awaiting results myself). Don't need to know names just looking for a ballpark figure by responses. Thanks Pam -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031207/9751030c/attachment.htm From ekaplan <@t> squ.edu.om Mon Dec 8 04:28:47 2003 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] water purification Message-ID: Good afternoon, I wonder if someone could tell me; What sort of conductivity should one obtain in water purification systems (deinoised water)for use in PCR? I would be much obliged if you could help out. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman From kemlo <@t> tiscali.co.uk Mon Dec 8 05:21:51 2003 From: kemlo <@t> tiscali.co.uk (Kemlo Rogerson) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Hardening very soft brains In-Reply-To: Message-ID: <003901c3bd7d$80daa400$b3062850@KEMLOS> Oh baby brains, I understand now. There was me thinking all sort of thoughts about sloppy brains and people I knew. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 01270 877625 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts:? ???????????? kemlo@tiscali.co.uk?or kemlo1@btinternet.com? Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. ? ? ? -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: 05 December 2003 16:27 To: Kemlo Rogerson; Geoff McAuliffe; Steve Machin UK Cc: Histonet Histonet Histonet Subject: RE: [Histonet] Hardening very soft brains Don't exaggerate Kemlo, we didn't use lead as a fixative. However we stained with lead haematoxylin (upside down) for APUD granules as they were then called, in carcinoids. BTW, for those not familiar with baby brains, they are like blancmange, or even runnier - horrid things to handle. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo@tiscali.co.uk] Sent: 05 December 2003 16:23 To: Marshall Terry Dr, Consultant Histopathologist; 'Geoff McAuliffe'; 'Steve Machin UK' Cc: 'Histonet Histonet Histonet' Subject: RE: [Histonet] Hardening very soft brains Yes I remember that with much pain; zinc, lead, you name it we fixed with it! If my memory serves me it made the nuclear stain rather dramatic too. Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Tel: 01270 877625 Mob: 07830 196072 Mobile E-Mail kemlorogerson@3mail.com FAX & Answer Phone 0871 242 8094 E-mail Accounts:? ???????????? kemlo@tiscali.co.uk?or kemlo1@btinternet.com? Disclaimer: The information contained in this message and/or any attachments(s) may be of a private and confidential nature, and is intended solely for the attention of the addressee. If you have received this message in error or feel you should not have been the intended recipient, please return it and any attachments to the sender immediately. All messages relating to this communication should then be deleted from your system. Unauthorised usage, copying, disclosure or alteration of this message and/or attachment(s) is strictly prohibited. Barking, Havering and Redbridge Hospitals NHS Trust will not be held responsible for any direct or indirect damages which may arise from alteration of this message or any attachment(s), by a third party or resulting from the transmission of a virus. ? ? ? -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: 05 December 2003 15:34 To: Geoff McAuliffe; Steve Machin UK Cc: Histonet Histonet Histonet Subject: RE: [Histonet] Hardening very soft brains Steve Machin UK wrote: >Could anyone help us with a problem we have in processing very soft >fetal brains? > >We currently fix in 20% formalin in buffer but the brains are so soft >after processing they stick to the wrapping paper. > >Any ideas on how we can harden them after fixation? I find zinc fixative totally brilliant for brain tissue, in both the hardening and morphologic aspects. You may not be able to keep the tissue on the slide, but heck, you can't have everything:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Mon Dec 8 09:55:40 2003 From: pathrm35 <@t> adelphia.net (Ron Martin) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) References: <3FD1025A.8040804@umdnj.edu> <3FD226AF.1050304@email.unc.edu> Message-ID: <003201c3bda3$bb15e8c0$ace23718@atlsfl.adelphia.net> >From my experience I can say that vet. pathology does not pay the same as human path. Also this is advertised as a technician position with no formal education or certification. I can also say from experience that many universities do not pay well at all. I'm sure that there is someone out there who cannot pass the HT exam, has no formal education and has little experience who will fulfill their requirements. You must also consider geographical location when considering salaries. Just my two cents worth. ----- Original Message ----- From: "Robert Schoonhoven" Cc: Sent: Saturday, December 06, 2003 10:57 AM Subject: Re: [Histonet] Temporary Histology Position Open (South Dakota State University) This must be some kind of joke.. My teenage son makes more at the local mall. Robert Schoonhoven Geoff McAuliffe wrote: > $9.39 an hour for a responsible, skilled postion? It's an insult. AND > I'll bet the administration gripes about "how hard it is to get good > help these days". Our Housekeeping staff starts at more than that, and > they have no training. > > SDSU HISTO wrote: > >> LABORATORY TECHNICIAN - VETERINARY SCIENCE >> >> >> >> This is a full-time, temporary position to last approximately three >> (3) months. >> >> >> >> Salary Range N11: $9.39 per hour >> >> >> >> POSITION PURPOSE: To prepare histology and immunohistochemistry >> slides for pathologic examination by faculty and to assist with >> general laboratory maintenance such as filing microscope slides and >> blocks, cleaning equipment and loading the tissue processor or >> automated stainers. >> >> >> KNOWLEDGE, SKILLS AND ABILITIES: >> >> Knowledge of: >> >> ? methods, materials, equipment and techniques of laboratory >> testing, analysis and media preparation; >> >> ? basic principles, practices, and procedures of laboratory >> testing; >> >> ? laboratory equipment, procedures, operation, and >> terminology; and >> >> ? safe laboratory practices; >> >> >> >> Skill and ability to: >> >> ? perform basic microtome; >> >> ? prioritize work; >> >> ? follow detailed directions and instructions; >> >> ? use and maintain laboratory equipment; >> >> ? perform laboratory tests or analysis; >> >> ? accurately record data onto computer; and >> >> ? lift up to 50 lbs. >> >> >> >> COMMENTS: Some work with potentially noxious chemicals is performed >> in the laboratory under a class II fume hood. >> >> >> Deadline: Open Until Filled >> >> >> >> Submit SD Job Application Form to: SDSU Human >> Resources >> >> >> Box 2201 >> >> >> Brookings, SD 57007 >> >> >> (605) 688-4128 >> >> >> >> >> >> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fmonson <@t> wcupa.edu Mon Dec 8 07:55:03 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] water purification Message-ID: Morning Evelyn, There should be NO conductivity and resistance, therefore, should be EXTREMELY high. For all molecular biology procedures that I can remember using, I redistilled deionized water in a scrupulously clean all glass still. Once I had a system, I used such water for all my histo- or cyto- preparations and methods, and reserved tap water for post-rinsing Schiff's reagent and final stabilization of H in H&E. With respect to deionizers, the ion trapping bed is generally specially chosen for the water that is to be processed, and thus, often does a more-than-adequate job. Cheers, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Evelyn Kaplan [mailto:ekaplan@squ.edu.om] Sent: Monday, December 08, 2003 5:29 AM To: Histonet Subject: [Histonet] water purification Good afternoon, I wonder if someone could tell me; What sort of conductivity should one obtain in water purification systems (deinoised water)for use in PCR? I would be much obliged if you could help out. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hadi83 <@t> comcast.net Mon Dec 8 08:18:36 2003 From: hadi83 <@t> comcast.net (Hadi Yaziji) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota State University) In-Reply-To: <003201c3bda3$bb15e8c0$ace23718@atlsfl.adelphia.net> References: <3FD1025A.8040804@umdnj.edu> <3FD226AF.1050304@email.unc.edu> <003201c3bda3$bb15e8c0$ace23718@atlsfl.adelphia.net> Message-ID: <69B9A46A-2989-11D8-9658-00039378E76A@comcast.net> I believe this is the largest histotechnology email list on record. It is interesting that few are justifying the race-to-the-bottom approach taken by the job ad. Someone who failed the HT exam and has no formal education and has little experience should be working at McDonald flipping burgers and not be sitting behind a microtome that he/she doesn't know how to use. Histotechs are as important as Medtechs, ICU nurses and other healthcare professionals who make decent money because the importance of their profession is more visible to the public than people in histology labs. I hope ads like that one won't be abundant. But it is probably the responsibility of the Histotechnology community (including NSH) to insure that the issue of pay is being monitored. There's no reason any licensed histotech should agree to such insulting job requirements. After all, there's more national shortage in histotech jobs than nursing jobs. This ad still sounds ironic to me, where the number of histotech schools that are currently operating keeps dropping. As pathologists (recently described as "elitists"), most of us feel that histotechs should be extremely well compensated in order for us to adequately carry out our daily practice. Hadi Yaziji, M.D. PhenoPath Laboratories On Dec 8, 2003, at 7:55 AM, Ron Martin wrote: > > From my experience I can say that vet. pathology does not pay the same > as > human path. Also this is advertised as a technician position with no > formal > education or certification. I can also say from experience that many > universities do not pay well at all. I'm sure that there is someone out > there who cannot pass the HT exam, has no formal education and has > little > experience who will fulfill their requirements. You must also consider > geographical location when considering salaries. Just my two cents > worth. > ----- Original Message ----- > From: "Robert Schoonhoven" > Cc: > Sent: Saturday, December 06, 2003 10:57 AM > Subject: Re: [Histonet] Temporary Histology Position Open (South Dakota > State University) > > > This must be some kind of joke.. My teenage son makes more at the > local mall. > > Robert Schoonhoven > > Geoff McAuliffe wrote: > >> $9.39 an hour for a responsible, skilled postion? It's an insult. AND >> I'll bet the administration gripes about "how hard it is to get good >> help these days". Our Housekeeping staff starts at more than that, and >> they have no training. >> >> SDSU HISTO wrote: >> >>> LABORATORY TECHNICIAN - VETERINARY SCIENCE >>> >>> >>> >>> This is a full-time, temporary position to last approximately three >>> (3) months. >>> >>> >>> >>> Salary Range N11: $9.39 per hour >>> >>> >>> >>> POSITION PURPOSE: To prepare histology and immunohistochemistry >>> slides for pathologic examination by faculty and to assist with >>> general laboratory maintenance such as filing microscope slides and >>> blocks, cleaning equipment and loading the tissue processor or >>> automated stainers. >>> >>> >>> KNOWLEDGE, SKILLS AND ABILITIES: >>> >>> Knowledge of: >>> >>> ? methods, materials, equipment and techniques of laboratory >>> testing, analysis and media preparation; >>> >>> ? basic principles, practices, and procedures of laboratory >>> testing; >>> >>> ? laboratory equipment, procedures, operation, and >>> terminology; and >>> >>> ? safe laboratory practices; >>> >>> >>> >>> Skill and ability to: >>> >>> ? perform basic microtome; >>> >>> ? prioritize work; >>> >>> ? follow detailed directions and instructions; >>> >>> ? use and maintain laboratory equipment; >>> >>> ? perform laboratory tests or analysis; >>> >>> ? accurately record data onto computer; and >>> >>> ? lift up to 50 lbs. >>> >>> >>> >>> COMMENTS: Some work with potentially noxious chemicals is performed >>> in the laboratory under a class II fume hood. >>> >>> >>> Deadline: Open Until Filled >>> >>> >>> >>> Submit SD Job Application Form to: SDSU Human >>> Resources >>> >>> >>> Box 2201 >>> >>> >>> Brookings, SD 57007 >>> >>> >>> (605) 688-4128 >>> >>> >>> >>> >>> >>> >>> >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mprice26 <@t> juno.com Mon Dec 8 09:56:02 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Histology Technician Shortage Message-ID: <20031208.075604.14426.163143@webmail22.nyc.untd.com> Hi histonetters, In the Dallas Morning News, (Sunday, Dec. 07, 2003) Page 3J. There is an interesting article about the National Crisis of the Shortage of Histology Technicians. It has nearly doubled from last year. The job vacancy rate is much higher than the Nurse Vacancy Rate. FYI Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From tpmorken <@t> labvision.com Mon Dec 8 10:35:29 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Goggling. verb....RE: Histology books and googling. Was Re: [Hist onet] Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CDE5@usca0082k03.rallansci.apogent.com> John wrote:<< Some months ago, a syndicated newspaper (print) columnist expounded in favour of a new verb, "to google," meaning to search the internet.>> John, Google is great, but it has become much more than that. Recently I asked my nephew to get his shoes on and he said "I have to google them." BTW, google "miserable failure" and click the "I'm feeling lucky" button to get a fantastic example of a "Google bomb." Tim Morken -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Friday, December 05, 2003 9:05 PM To: Christian Franci Cc: histonet@lists.utsouthwestern.edu Subject: Histology books and googling. Was Re: [Histonet] Christian Franci wrote: > > Can anyone suggest a good, basic but thorough, histology book? ----------------------------------------- You really should put _something_ in the Subject line! I've supplied a crazy long one for this reply. Histology books. I recommend Wheater's Functional Histology: A Text and Colour Atlas (Book with CD-ROM) by Barbara Young (Editor), John W. Heath (Editor), Churchill Livingstone. US$59.95 from amazon.com Junqueira's Basic Histology: Text & Atlas, 10th Edition (also with a CD) is also very good. Both books have lots of coloured pictures of sections stained with a variety of techniques in addition to haemalum & eosin. Wheater's has the descriptive text closely tied to the illustrations. The text of Junqueira is, perhaps, more thorough. The CDs with both books are rather poorly organized and troublesome to use. Among other things they have unlabelled versions of the illustrations that can be used in teaching lectures. I don't teach histology, so I don't know how valuable such a collection would be. My histology-teaching colleagues have their own collections of photomicrographs of 35mm slides, made from stained sections similar to those used in the lab classes. For a histology course without labs, taught by faculty members deprived of microscope, slides and camera, a CD of good photos may be a necessity. This already is The Shape of Things to Come in medical education. Why fiddle with the condenser and the fine focus when you can use your inkjet printer to make a copy of some expert's best picture? It is a frightening prospect that in the future histology may be taught by people who have not looked at sections with a microscope. Next, a brighter prospect! Googling. Isn't Google wonderful! While typing this reply I googled a two-word query for Wheater's histology, and the book's amazon.com page came up (at the top of a long list) in about 5 seconds, and that's by way of a telephone line modem connection from home. For quite specialized literature searches Google is often faster than PubMed or Web of Science, and it can quickly take you to the full text version of an article. (This is probably because my institution subscribes to the on-line versions of many journals. I doubt if it would work from a hotmail address! Nevertheless, abstracts of all biomedical papers are freely available to all by way of PubMed, which is rapidly and almost intelligently searched by Google.) Possibly some of the other big search engines are as good as Google for finding scientific literature. I've tried 2 or 3 others, and they were useless; worse than they were 2 years ago because clogged up with interuptions by advertisers. Some months ago, a syndicated newspaper (print) columnist expounded in favour of a new verb, "to google," meaning to search the internet. For what it's worth, I support this new verb, and place it along with "to hoover," which concisely means to push a vacuum cleaner over a carpet. Getting a bit off topic, unless there are some histonetters who cut and stain sections of carpets ... Perhaps that's enough. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trapani <@t> spot.colorado.edu Mon Dec 8 10:50:07 2003 From: trapani <@t> spot.colorado.edu (Josh Trapani) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] RE: cryosectioning adult fish teeth? In-Reply-To: References: Message-ID: Dear All, I would like to thank everyone who replied to my request last week for some advice on cryosectioning pharyngeal teeth of adult fish. If you are curious as to the responses, I had at least three people recommend using tape-transfer methods. These are new to me, and I'm starting to do some research on them. Someone else also recommended a modified freezing/cutting procedure; in particular, freezing the specimen slowly (little by little, that is) in a rectangular mold and then positioning the edge of the mold parallel to the blade (I had been doing quick - all at once - freezing in circular molds). I haven't tried this yet, but will within in a few days when I get back on the machine. Again, thank you for your help. Josh Trapani University of Colorado From tpmorken <@t> labvision.com Mon Dec 8 10:54:46 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota St ate University) Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CDE6@usca0082k03.rallansci.apogent.com> RE: pay for vet/academic lab techs. I don't begrudge the pay they offer. It has always been and always will be low at colleges because they have a pool of people willing to work for absolute minimum wage - the lowly undergrad. True, you can get a mall or temp job for that in many places (but probably not in South Dakota!). I still see many ads, even for EM techs, in this range. I once called into question the pay for an "entry level EM tech" at a very prestigious university, a position I assumed would require a solid theoretical background at the least (especially considering that the researchers probably know next to nothing about EM), and was told "... it's not rocket science; any undergrad can do it, so why pay more than they would make at the local mall?" This for a job in which a certain amount of skill was "..desired.." according to the ad. Last I heard, a mall job requires no skill at all, at least nothing requiring more than 10 minutes of instruction. I think this was pretty much in the old style of pathology in which strangers were dragged off the street and taught to cut and stain, and never did figure out exactly what they were doing. Tim Morken -----Original Message----- From: Paul Howard Lockwood [mailto:tigersnake@ecybermind.net] Sent: Sunday, December 07, 2003 3:44 PM To: Marsha R Price; rschoon@email.unc.edu Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] Temporary Histology Position Open (South Dakota State University) To All, Having read the ad for the temp position, I have to agree that it is an insult. They must be trying to get someone very desperate for a job, or they hope to hire someone just graduated from college, or a tech school, who doesn't know any better. Just thought I'd add my two copper coins worth. Paul Lockwood 12/7/03 8:11:08 AM, Marsha R Price wrote: >Surely it is a joke. They must not be trying to hire a registered >Histotech. >Marsha Price > >On Sat, 06 Dec 2003 13:57:51 -0500 Robert Schoonhoven > writes: >> This must be some kind of joke.. My teenage son makes more at the >> local mall. >> >> Robert Schoonhoven >> >> Geoff McAuliffe wrote: >> >> > $9.39 an hour for a responsible, skilled postion? It's an insult. >> AND >> > I'll bet the administration gripes about "how hard it is to get >> good >> > help these days". Our Housekeeping staff starts at more than that, >> and >> > they have no training. >> > >> > SDSU HISTO wrote: >> > >> >> LABORATORY TECHNICIAN - VETERINARY SCIENCE >> >> >> >> >> >> >> >> This is a full-time, temporary position to last approximately >> three >> >> (3) months. >> >> >> >> >> >> >> >> Salary Range N11: $9.39 per hour >> >> >> >> >> >> >> >> POSITION PURPOSE: To prepare histology and immunohistochemistry >> >> >> slides for pathologic examination by faculty and to assist with >> >> general laboratory maintenance such as filing microscope slides >> and >> >> blocks, cleaning equipment and loading the tissue processor or >> >> automated stainers. >> >> >> >> >> >> KNOWLEDGE, SKILLS AND ABILITIES: >> >> >> >> Knowledge of: >> >> >> >> * methods, materials, equipment and techniques of >> laboratory >> >> testing, analysis and media preparation; >> >> >> >> * basic principles, practices, and procedures of >> laboratory >> >> testing; >> >> >> >> * laboratory equipment, procedures, operation, and >> >> terminology; and >> >> >> >> * safe laboratory practices; >> >> >> >> >> >> >> >> Skill and ability to: >> >> >> >> * perform basic microtome; >> >> >> >> * prioritize work; >> >> >> >> * follow detailed directions and instructions; >> >> >> >> * use and maintain laboratory equipment; >> >> >> >> * perform laboratory tests or analysis; >> >> >> >> * accurately record data onto computer; and >> >> >> >> * lift up to 50 lbs. >> >> >> >> >> >> >> >> COMMENTS: Some work with potentially noxious chemicals is >> performed >> >> in the laboratory under a class II fume hood. >> >> >> >> >> >> Deadline: Open Until Filled >> >> >> >> >> >> >> >> Submit SD Job Application Form to: SDSU >> Human >> >> Resources >> >> >> >> >> >> >> Box 2201 >> >> >> >> >> >> >> Brookings, SD 57007 >> >> >> >> >> >> >> (605) 688-4128 >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> > >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >________________________________________________________________ >The best thing to hit the internet in years - Juno SpeedBand! >Surf the web up to FIVE TIMES FASTER! >Only $14.95/ month - visit www.juno.com to sign up today! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From StarkusL <@t> ummhc.org Mon Dec 8 11:01:26 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota St ate University) Message-ID: There is still that mentality going on in the Mohs field. Medical assistants are being taught how to cut and stain. Then, when something goes wrong, they have no idea how to handle it. Two years ago, I went to work for a prestigious hospital in Boston and discovered that the mohs tech there was nothing more than an MA who had been taught how to cut. When something happened with the stain the solution was to dump everything out, order new stain and start again. I kid you not. Consequently, there are some Mohs "techs" out there who are making approximately that amount of money. -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Monday, December 08, 2003 11:55 AM To: histonet@pathology.swmed.edu Subject: RE: [Histonet] Temporary Histology Position Open (South Dakota St ate University) RE: pay for vet/academic lab techs. I don't begrudge the pay they offer. It has always been and always will be low at colleges because they have a pool of people willing to work for absolute minimum wage - the lowly undergrad. True, you can get a mall or temp job for that in many places (but probably not in South Dakota!). I still see many ads, even for EM techs, in this range. I once called into question the pay for an "entry level EM tech" at a very prestigious university, a position I assumed would require a solid theoretical background at the least (especially considering that the researchers probably know next to nothing about EM), and was told "... it's not rocket science; any undergrad can do it, so why pay more than they would make at the local mall?" This for a job in which a certain amount of skill was "..desired.." according to the ad. Last I heard, a mall job requires no skill at all, at least nothing requiring more than 10 minutes of instruction. I think this was pretty much in the old style of pathology in which strangers were dragged off the street and taught to cut and stain, and never did figure out exactly what they were doing. Tim Morken -----Original Message----- From: Paul Howard Lockwood [mailto:tigersnake@ecybermind.net] Sent: Sunday, December 07, 2003 3:44 PM To: Marsha R Price; rschoon@email.unc.edu Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] Temporary Histology Position Open (South Dakota State University) To All, Having read the ad for the temp position, I have to agree that it is an insult. They must be trying to get someone very desperate for a job, or they hope to hire someone just graduated from college, or a tech school, who doesn't know any better. Just thought I'd add my two copper coins worth. Paul Lockwood 12/7/03 8:11:08 AM, Marsha R Price wrote: >Surely it is a joke. They must not be trying to hire a registered >Histotech. >Marsha Price > >On Sat, 06 Dec 2003 13:57:51 -0500 Robert Schoonhoven > writes: >> This must be some kind of joke.. My teenage son makes more at the >> local mall. >> >> Robert Schoonhoven >> >> Geoff McAuliffe wrote: >> >> > $9.39 an hour for a responsible, skilled postion? It's an insult. >> AND >> > I'll bet the administration gripes about "how hard it is to get >> good >> > help these days". Our Housekeeping staff starts at more than that, >> and >> > they have no training. >> > >> > SDSU HISTO wrote: >> > >> >> LABORATORY TECHNICIAN - VETERINARY SCIENCE >> >> >> >> >> >> >> >> This is a full-time, temporary position to last approximately >> three >> >> (3) months. >> >> >> >> >> >> >> >> Salary Range N11: $9.39 per hour >> >> >> >> >> >> >> >> POSITION PURPOSE: To prepare histology and immunohistochemistry >> >> >> slides for pathologic examination by faculty and to assist with >> >> general laboratory maintenance such as filing microscope slides >> and >> >> blocks, cleaning equipment and loading the tissue processor or >> >> automated stainers. >> >> >> >> >> >> KNOWLEDGE, SKILLS AND ABILITIES: >> >> >> >> Knowledge of: >> >> >> >> * methods, materials, equipment and techniques of >> laboratory >> >> testing, analysis and media preparation; >> >> >> >> * basic principles, practices, and procedures of >> laboratory >> >> testing; >> >> >> >> * laboratory equipment, procedures, operation, and >> >> terminology; and >> >> >> >> * safe laboratory practices; >> >> >> >> >> >> >> >> Skill and ability to: >> >> >> >> * perform basic microtome; >> >> >> >> * prioritize work; >> >> >> >> * follow detailed directions and instructions; >> >> >> >> * use and maintain laboratory equipment; >> >> >> >> * perform laboratory tests or analysis; >> >> >> >> * accurately record data onto computer; and >> >> >> >> * lift up to 50 lbs. >> >> >> >> >> >> >> >> COMMENTS: Some work with potentially noxious chemicals is >> performed >> >> in the laboratory under a class II fume hood. >> >> >> >> >> >> Deadline: Open Until Filled >> >> >> >> >> >> >> >> Submit SD Job Application Form to: SDSU >> Human >> >> Resources >> >> >> >> >> >> >> Box 2201 >> >> >> >> >> >> >> Brookings, SD 57007 >> >> >> >> >> >> >> (605) 688-4128 >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> > >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > >________________________________________________________________ >The best thing to hit the internet in years - Juno SpeedBand! >Surf the web up to FIVE TIMES FASTER! >Only $14.95/ month - visit www.juno.com to sign up today! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Mon Dec 8 11:08:41 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open (South Dakota St ate University) Message-ID: Can't resist my opinion. Rural America and for that matter the inner cities are a world apart. Here in Montana although many of us are somewhat well paid the pay scale is below most places and I imagine South Dakota is below Montana - just a guess. There is a pervasive mentality that if you can't survive you do not belong here. I see a far greater difference between the haves and have nots. In addition I have a daughter living and working in NYC that feels lucky to have a job in her profession[photography] at $6.00/hour just to get her foot in the door, and yes she is very well educated and certified etc! Low pay scales and the investment market means I will be working for a long time and happy if I can keep my job! Happy Holidays to all!!! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Robert Schoonhoven Sent: Saturday, December 06, 2003 11:58 AM Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Temporary Histology Position Open (South Dakota State University) This must be some kind of joke.. My teenage son makes more at the local mall. Robert Schoonhoven Geoff McAuliffe wrote: > $9.39 an hour for a responsible, skilled postion? It's an insult. AND > I'll bet the administration gripes about "how hard it is to get good > help these days". Our Housekeeping staff starts at more than that, and > they have no training. > > SDSU HISTO wrote: > >> LABORATORY TECHNICIAN - VETERINARY SCIENCE >> >> >> >> This is a full-time, temporary position to last approximately three >> (3) months. >> >> >> >> Salary Range N11: $9.39 per hour >> >> >> >> POSITION PURPOSE: To prepare histology and immunohistochemistry >> slides for pathologic examination by faculty and to assist with >> general laboratory maintenance such as filing microscope slides and >> blocks, cleaning equipment and loading the tissue processor or >> automated stainers. >> >> >> KNOWLEDGE, SKILLS AND ABILITIES: >> >> Knowledge of: >> >> ? methods, materials, equipment and techniques of laboratory >> testing, analysis and media preparation; >> >> ? basic principles, practices, and procedures of laboratory >> testing; >> >> ? laboratory equipment, procedures, operation, and >> terminology; and >> >> ? safe laboratory practices; >> >> >> >> Skill and ability to: >> >> ? perform basic microtome; >> >> ? prioritize work; >> >> ? follow detailed directions and instructions; >> >> ? use and maintain laboratory equipment; >> >> ? perform laboratory tests or analysis; >> >> ? accurately record data onto computer; and >> >> ? lift up to 50 lbs. >> >> >> >> COMMENTS: Some work with potentially noxious chemicals is performed >> in the laboratory under a class II fume hood. >> >> >> Deadline: Open Until Filled >> >> >> >> Submit SD Job Application Form to: SDSU Human >> Resources >> >> >> Box 2201 >> >> >> Brookings, SD 57007 >> >> >> (605) 688-4128 >> >> >> >> >> >> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Mon Dec 8 11:18:47 2003 From: TJasper <@t> smdc.org (Jasper, Thomas) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] SDSU HISTO Temp position Message-ID: <1DB04B57B04C5747B87C3B39F3E605AA23FD1C@harrier> I have waited a bit to comment as my initial reaction was one of disbelief. It is this type of nonsense that only worsens the vacancy rate the world of histology suffers from. I looked closely at the job description again to be sure I didn't misunderstand anything. The statement about preparing IHC for path examination is an indication to me that those expecting a qualified response have no respect for our profession. It would also seem that these same individuals are woefully lacking in knowledge of basic economics. I work in Minnesota and although we are not Brookings, S.D. we are in a relatively similar economic area as we are not on either coast or in some huge metropolitan area. I also have previous experience working in vet. pathology and 20 years ago people were being paid in this salary range (relative to region of course). I'm quite certain Ronald Reagan is no longer president even in Brookings and the comment about noxious chemicals hopefully doesn't apply to benzene. I think I can predict with some certainty that the full-time temporary position will expire before it is filled. That is of course unless someone wants to take a leave of absence from delivering pizzas (I used to do that too!) and then return to their permanent employment once SDSU is done using them. Thomas G. Jasper Anatomic Pathology Coordinator SMDC Duluth, MN 55805 (218) 786-4510 tjasper@smdc.org From brett_connolly <@t> merck.com Mon Dec 8 11:48:45 2003 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histology Position Open Message-ID: I think this one has been sufficiently beaten to death. Let's put the clubs away and move on. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com From asmith <@t> mail.barry.edu Mon Dec 8 12:25:33 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] water purification Message-ID: <494304423C63E246A5CF87A3AEEB577011B5B2@bumail01.barrynet.barry.edu> A reasonable resistance for highly purified water would be about 1 megaohm/cm. Thus the conductivity should be about 0.000001 mho . cm. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Evelyn Kaplan Sent: Monday, December 08, 2003 5:29 AM To: Histonet Subject: [Histonet] water purification Good afternoon, I wonder if someone could tell me; What sort of conductivity should one obtain in water purification systems (deinoised water)for use in PCR? I would be much obliged if you could help out. Regards, Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From bill501 <@t> mindspring.com Mon Dec 8 13:09:54 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Change from digest to individual emails In-Reply-To: References: Message-ID: How do I change from digest to individual emails on this list? TIA, Bill -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) http://www.druidry.org From bill501 <@t> mindspring.com Mon Dec 8 13:28:58 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Re: Stain for H.Pylori In-Reply-To: <1e6.14e8e8c4.2d04cc5e@aol.com> References: <1e6.14e8e8c4.2d04cc5e@aol.com> Message-ID: I think the most important thing with the non-IHC syains is good fixation of the mucin. We found that going from fomalin to Zn formalin years ago made a big difference. Otherwise the mucin gets stringy. and where strings cross can look like bacteria. With a good specimen plus a good Giemsa, I can do most antrum bxs with high dry. An occasional case requires oil, esp if there are a lot of other bacteria present coming from the oropharynx or if the preparation is suboptimal. I like the Alcian yellow better, but it is considerably more expensive for us to do. Bill -- ______________ Bill Blank, MD Heartland Lab, Inc From phyllitis <@t> hotmail.com Mon Dec 8 13:53:49 2003 From: phyllitis <@t> hotmail.com (MK) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] unsubscribe References: Message-ID: ----- Original Message ----- From: "HistoNet Server" To: "* *" Sent: Thursday, June 05, 2003 2:05 PM Subject: re: subscribe > Your address has been added to the addresses that comprise this Listserv > List. > Welcome to HISTONET. This is an electronic mailing list for the exchange of > information pertaining to histotechnology and related fields. > > PLEASE SAVE THIS MESSAGE. > It contains useful information about how to use the list and what to do if you > experience problems. It also includes some basic rules for email etiquette > (Netiquette) which will be helpful to those who are new to this form of > communication. > > WHAT IS A LISTSERVER? > A list server is a computer that runs software which will receive incoming > electronic mail (email) messages and reroute them automatically to everyone on > the subscriber list. Email uses the vast expanse of the Internet to allow > almost instantaneous communication between networked computers around the > world. Our system uses the LISTSTAR software from Quarterdeck Corporation > (California) and can currently send about 30 messages a minute. With the > present number of subscribers, we are processing about 10,000 outbound > messages a day. > > WHO SHOULD SUBSCRIBE? > Anyone interested in research or clinical applications of histology, > immunohistochemistry, in-situ hybridization pathology, and electron microscopy > may find Histonet informative and useful. Currently, there are more than 850 > subscribers from all over the world. Subscribers include hospital employees > from major urban centers and obscure remote locales, university researchers, > botanists and the employees of commercial laboratories, government agencies, > veterinary facilities and a wide variety of commercial industrial ventures. > > WHO RUNS HISTONET? > The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using > hardware and software owned by the University of Texas Southwestern Medical > School, Department of Pathology in Dallas, Texas. If you have any questions or > problems with Histonet please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us. > > HOW DOES THE LIST WORK? > This server, unlike many systems, uses ONLY ONE ADDRESS to send commands to > the computer and to post messages. The server will recognize commands sent in > the SUBJECT line of the message and only when they are spelled exactly as > listed below. Anything not identified as a command will be circulated to > EVERYONE on the list. > > The following is a list of commands the server recognizes: > > subscribe > Your address will be added to the list of subscribers. You will then be able > to send messages to this list that will be forwarded to all other list > subscribers. You will begin to receive all messages sent to the list by other > subscribers. > > subscribe digest > Your address will be added to the list of subscribers who receive a digest > instead of each forwarded message. A digest is a compilation of all the > messages received in a 24 hour period. It is sent to the digest subscribers > every night after midnight. Digest subscribers can post and respond to > messages the same as "real-time" subscribers. > > digests > A list of available digests will be returned to you. Histonet stores old > messages as daily digests for approximately three months. To read previous > messages, copy the list of available digests, mark the dates of interest and > return it to the server. > > unsubscribe > Your address will be removed from the list of subscribers. > You will no longer be able to send messages to the members > of the list. > > help > A list of the commands recognized by the server will be returned to you. > > WHAT ARE THE RULES? > You may post any questions you wish pertaining to histology, pathology, > in-situ hybridization, immunohistochemistry etc. Equipment and reagent > evaluations, laboratory management issues, government regulations, and job > opportunities are all appropriate topics. The University asks that we restrict > the use of its hardware and software to business purposes only (occasional > jokes do slip through but PLEASE use restraint). Vendors and those with > commercial interests in histology products are welcome contributors however, > we ask that blatant advertisements be avoided at all times. It is fine to > refer to product that your company produces if it is pertinent to a topic > being discussed on the list. Unsolicited advertisements are poorly tolerated > by the members and you will likely receive a number of negative comments if > you overstep the boundaries. Please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us if you are not sure about the appropriateness > if a message you wish to post. > > > BASIC HISTONET "NETIQUETTE" > It is most helpful to the list members if you post your responses to queries > to everyone on the list and not just as a personal reply to the person asking > the question. That way duplicate messages are minimized and we all learn from > each other's comments. > > Likewise, if you post a question and get a number of responses back directly > to you, it is helpful to everyone if you could send out a summary of the > replies you got to Histonet. > > Please avoid abbreviations unless they are explained in your message. For > example: immunohistochemistry (IHC). This list circulates to a wide variety of > individuals and what seems obvious to you may have no meaning on the other > side of the world. > > Please sign your letter and include your institution or affiliation and > location. Not all email systems have headers which identify the sender. > > Do use the subject line to indicate the topic of your message. > > DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. > > Please send questions and problems about the list directly to Linda Margraf at > LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 > subscribers on the list. Be careful when sending commands to the server to put > the command in the SUBJECT LINE and spell it correctly. > > Please do not send images as attachments with your message. We can now post > images at our web site (http://pathcuri1.swmed.edu). To have an image posted > send it to Herb Hagler at herb.hagler@email.swmed.edu. > > > > > es > > From TrogadisJ <@t> smh.toronto.on.ca Mon Dec 8 14:45:35 2003 From: TrogadisJ <@t> smh.toronto.on.ca (Judy Trogadis) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] FISH and IF Message-ID: Fellow histologists Can anyone advise me about a protocol for combined FISH (for mRNA) and IF (for protein)? Many of the references don't include fluorescence for mRNA detection but I would really like to use our confocal microscope. As a philosophical issue, apart from amplification of the expression (prehybridization) or of the signal (post hybridization) has in situ hybridization itself changed much in the last few years? In other words, how valid are references to "critical steps" from the early 90's? Because there seems to be more pure methods papers, carefully testing each step, from that time then currently. Thank you for any advice and I look forward to opinions. Judy Judy Trogadis Bio-Imaging Coordinator St. Michael's Hospital, 7Queen 30 Bond St. Toronto, ON M5B 1W8 Canada ph: 416-864-6060 x6337 pager: 416-685-9219 fax: 416-864-6043 trogadisj@smh.toronto.on.ca From lleach <@t> uic.edu Mon Dec 8 15:00:10 2003 From: lleach <@t> uic.edu (Lu Leach) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Collagen III antibody Message-ID: <5.1.1.5.2.20031208145623.00aa4638@tigger.uic.edu> Dear Netters, Does anyone have a suggestion for an antibody to Collagen III in FFPE tissue? I could really use some help in locating a good antibody. Thank you, Lu Leach University of Illinois at Chicago lleach@uic.edu 312-996-3869 From algranth <@t> u.arizona.edu Mon Dec 8 16:03:56 2003 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] need help finding a Luna Stain procedure Message-ID: <4.3.2.7.2.20031208143021.00cdeea8@algranth.inbox.email.arizona.edu> Happy Monday! I'm looking for the procedure to do a Luna stain. We have some skin samples probably with sun damage and we want to look at the grenz zone in them. A Luna stain for elastin was discussed in April, 03 on histonet but a search of the archives did not turn up the procedure. I found a Gomori Aldehyde Fuchsin/Trichrome by Lee Luna and Darryl Luna in an old HistoLogic but I'm not sure that this is the same stain. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From pruegg <@t> colobio.com Mon Dec 8 17:57:39 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] collagen In-Reply-To: Message-ID: Megan, I would first do a stain for proteoglycan, something like 0.2% Azure B ph 6 to see what is there. Just stain with this for 5 min., rinse in dih20 airdry and coverslip. This will demonstrate everything from collagen to cartilage to bone formation and is very simple. If you want to get to classifying the collagen type I recommend using the antibodies from U of Iowa Hybridoma Bank, they have all kinds of collagens. Collagen antibodies can be obtained from Developmental Studies Hybridoma Bank, Department of Biological Sciences, University of Iowa, 007 BBE, IowA City, IA 52242-1324 tele: 319-335-3826, fax 319-335-2077 email dshb@uiowa.edu Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Megan Kear Sent: Sunday, December 07, 2003 6:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] collagen Hi , could someone help with a recommendation for staining "newly laid colagen",we think it is type v but not quite sure.Any suggestions would be of great help. Thank You Megan Kear HAPS Newcastle Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Mon Dec 8 18:22:25 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Temporary Histo Position at SDSU-my comments In-Reply-To: Message-ID: I always said "I am not in it for the money". I love my work in histology and could have been wealthier doing something else. I may not be dollar rich but I have been blessed with richness by feeling like what I do makes a difference in the world. I sit here today with big snow coming down outside, (I should have left hours ago to get home safe and secure) yet I stay to finish some IHC because I know this will make a difference in this patients life. In 1976 I started working at the U of Colorado as a registered HT for $600. a month being happy to have the learning and giving experience. Best regards, Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Barbara Stancel Sent: Sunday, December 07, 2003 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temporary Histo Position at SDSU-my comments Dear SDSU Histo, I feel so for you and your plight to obtain pretrained histology help for a veterinary histology position, temporary or full time. Working in a university setting can be tough. Pay is crappy. Days can be long. And even though the description may say "histology position" they usually tack on " and other duties as assigned" which may include necropy duty and in some research areas, the heart wrenching job of helping with euthanasia. The perks may include good health/dental insurance, set hours, no weekend work, an avenue of new experiences, and possibly the opportunity to work with graduate students and veterinary professors. Some of them are wonderful and others wear their multitude of degrees as body armour. It provides them protection from the backlash of punishments of poor or misguided decisions. For the most part my experiences (in the trenches of the lowest paid position in the Pathology/Parasitology department in a university setting) were positive. I was fortunate to work with researchers who appreciated my work and were not above asking for my input on research projects. But in the 1970's and 80's, the general montra was "The pay is bad, but we can't do anything about that. Besides, we can always find a student's spouse who will be glad to get the money." And usually they were right. I agree with all those who have condemned the pay per hour. It is bad. But when the personnel and admistration say that is all they will pay......that's it. It was embarrassing to advertise my low paying positions, but what choices did I have. It can be disheartening to send a temp job notice to the HISTONET and then get "blasted" for advertising a pay scale you have no control over, but desparately need the help. Hopefully you can print out the many negative responses for your supervisor. Maybe even talk to local establishments and find out their pay scales for employees. Comparing to the local hospitals may not be beneficial since most vet universities refuse to be compared to human workers. Or how about contacting some of the temp agencies for lab personnel. See how much it would cost to hire a temp through an agency. Take this information to your supervisor or personnel department. I will be retiring in a few years. I will probably continue to stay involved in some way with lab work. At that time, I may even be tempted to travel and take temporary positions for the sake of seeing different areas of the country.......but not South Dakota in the winter!!!! Best of Luck, Histologically yours, Barbara in Athens, Georgia (Where our coldest days are in the 20's. Snow-days are may count in twos and threes per year, AND they call off schools and work!! I can't drive in it. I don't own snow tires. Tire chains tear up our roads. And I want to stay at home a day and enjoy the beauty of snow, even when it comes with ice and sleet and downed power lines.) ________________________________________________________ LABORATORY TECHNICIAN - VETERINARY SCIENCE - This is a full-time, temporary position to last approximately three (3) months. Salary Range N11: $9.39 per hour POSITION PURPOSE: To prepare histology and immunohistochemistry slides for pathologic examination by faculty and to assist with general laboratory maintenance such as filing microscope slides and blocks, cleaning equipment and loading the tissue processor or automated stainers. KNOWLEDGE, SKILLS AND ABILITIES: Knowledge of methods, materials, equipment and techniques of laboratory testing, analysis and media preparation; basic principles, practices, and procedures of laboratory testing laboratory equipment, procedures, operation, and terminology; and safe laboratory practices; Skill and ability to perform basic microtome; prioritize work; follow detailed directions and nstructions; use and maintain laboratory equipment; perform laboratory tests or analysis;accurately record data onto computer; and lift up to 50 lbs. _________________________________________________________________ Cell phone 'switch' rules are taking effect - find out more here. http://special.msn.com/msnbc/consumeradvocate.armx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WWmn916 <@t> aol.com Mon Dec 8 20:47:07 2003 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] COX stain for muscle Message-ID: <4295DC55.2F5D0B52.001A2AA1@aol.com> Hi everyone, Say would anyone have a good stain procedure for the COX stain for frozen muscle bx's? Thanks Deb King, HT Sacramento, CA From frouwke <@t> sci.kun.nl Tue Dec 9 03:09:09 2003 From: frouwke <@t> sci.kun.nl (Frouwke Kuijpers) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] using "antifreeze" for section storage Message-ID: <004001c3be34$1c627fe0$4a8aae83@sci.kun.nl> Hallo, A few month ago I had a question about storage in an antifreeze medium : 0,05 Sodium phosphate buffer, 30% ethylen glycol, 20% glycerol for storing 40 micron sections at - 20 C. Well, we stored the sections like this way and now we want to use them for staining. But: when we put the sections from the antifreeze in the PBS buffer it became thick and slimy. After a long washing in the PBS the slimy thing has gone but when we take the sections on the brush it "reacts" in the air, putting them back in the buffer it seems normal again. Can anyone tell me what this reaction can be? Will the sections still be good enough for in situ? What is the right treatment after the antifreeze storage, thorough washing in AD. f.e. ? Thank you Frouwke Kuijpers F.J.Kuijpers-Kwant Dept. Cellular Animal Physiology University of Nijmegen Toernooiveld 1 6525 ED Nijmegen frouwke@sci.kun.nl From cgfields <@t> lexhealth.org Tue Dec 9 07:41:39 2003 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Histology Technician Shortage Message-ID: THX Marsha, I had a tech turn in her resignation yesterday and it is going to be tough to find a tech for what we pay...it isn't $9... thank goodness. I thought the going rate for a Histo tech however should be at least $19 to start and that is low....and we pay lower than that!!! Any help I can get is appreciated. Carole Fields Lex. Med. Ctn W.Columbia, SC > -----Original Message----- > From: mprice26@juno.com [SMTP:mprice26@juno.com] > Sent: Monday, December 08, 2003 5:09 PM > To: Carole Fields > Subject: RE: [Histonet] Histology Technician Shortage > > > Carole, > I am going to scan and e-mail to everyone tomorrow. I couldn't pull up on > their website either. > > Marsha > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/dead92b9/attachment.htm From mprice26 <@t> juno.com Tue Dec 9 08:46:21 2003 From: mprice26 <@t> juno.com (Marsha Price) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Marsha Price has something for you... Message-ID: <1020730.1070981181539.JavaMail.resin@hera.belointeractive.com> An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/85ae433a/attachment.htm From livieira <@t> ualg.pt Tue Dec 9 08:47:00 2003 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Make of Toluidine blue solution Message-ID: <001e01c3be63$4d66ac40$2914100a@labhistologia> Helo Everyone, In our lab usually, we make the toluidine solution (for coloration of resin included tissue): 1g - Borax 1g - Toluidine Blue 100 - ml water, 1 minute - staining time And because, now, I have another protocol for make the solution, I want ask you about the possibility to use this solution, and if is it necessary change the staining time? New protocol: 0.2 g - Toluidine Blue 0.5 - Acetic acid Complete, with desionized water, to 100 ml Tanks in advance Lina Vieira -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/6bae5c41/attachment.htm From mprice26 <@t> juno.com Tue Dec 9 08:51:23 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Histo Tech Shortage Article Message-ID: <20031209.065206.5792.851147@webmail23.nyc.untd.com> Histonetters, I was able to pull up the article on the Dallas Morning News website this morning. I e-mailed to the list. If you did not get it you can go to: 1.www.dallasmorningnews.com 2. Click on Classified in the top right corner. 3. Click on Job Center. 4. Scroll down untill you see article titled "Histotechnologists aim to stir interest in field." On left side of page. 5. Click on that article and you can read or print article. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From mprice26 <@t> juno.com Tue Dec 9 09:04:39 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] {Histonet} Marsha Price Has Something For You Message-ID: <20031209.070532.5792.851371@webmail23.nyc.untd.com> Hi Histonetters, This is the article in the Sundays edition of the Dallas Morning News about the Histology Tech. Shortage I e-mailed the list about. Let me know if you received o.k. For those that did not want a copy. You can just hit your delete key. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From mcauliff <@t> umdnj.edu Tue Dec 9 09:26:51 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Make of Toluidine blue solution In-Reply-To: <001e01c3be63$4d66ac40$2914100a@labhistologia> References: <001e01c3be63$4d66ac40$2914100a@labhistologia> Message-ID: <3FD5E9BB.2090406@umdnj.edu> Hi Lina: The borax makes the stain alkaline so it will penetrate the epoxy. Acetic acid will make the stain acidic, I don't know how well it will penetrate the epoxy (probably not very well which is why people have been putting borax in their toluidine blue since 1961 or so). If you have a protocol that works, why change? Geoff Lina Vieira wrote: > Helo Everyone, > > In our lab usually, we make the toluidine solution (for coloration of > resin included tissue): > 1g - Borax > 1g - Toluidine Blue > 100 - ml water, > > 1 minute - staining time > > And because, now, I have another protocol for make the solution, I > want ask you about the possibility to use this solution, and if is it > necessary change the staining time? > > New protocol: > 0.2 g - Toluidine Blue > 0.5 - Acetic acid > Complete, with desionized water, to 100 ml > > > Tanks in advance > > Lina Vieira > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From ander093 <@t> gold.tc.umn.edu Tue Dec 9 10:24:33 2003 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Histo Tech Shortage Article In-Reply-To: <20031209.065206.5792.851147@webmail23.nyc.untd.com> Message-ID: <5.2.0.9.0.20031209102303.00a1d8a0@ander093.email.umn.edu> Thanks Marcia!!! I tryed to find it yesterday, but was unsuccessful. Thanks for sending it to the list. LuAnn At 02:51 PM 12/9/03 +0000, you wrote: >Histonetters, >I was able to pull up the article on the Dallas Morning News website this >morning. > >I e-mailed to the list. If you did not get it you can go to: >1.www.dallasmorningnews.com >2. Click on Classified in the top right corner. >3. Click on Job Center. >4. Scroll down untill you see article titled "Histotechnologists aim to >stir interest in field." On left side of page. >5. Click on that article and you can read or print article. > >Marsha Price > >________________________________________________________________ >The best thing to hit the internet in years - Juno SpeedBand! >Surf the web up to FIVE TIMES FASTER! >Only $14.95/ month - visit www.juno.com to sign up today! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Tue Dec 9 10:25:30 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] RE: purchase of warming block Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/1883c72b/attachment.htm From jkiernan <@t> uwo.ca Tue Dec 9 10:58:59 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Make of Toluidine blue solution References: <001e01c3be63$4d66ac40$2914100a@labhistologia> Message-ID: <3FD5FF53.37F5356C@uwo.ca> The first solution (with borax) is alkaline, so it will stain everything in the section. This is usually what you want for thin sections embedded in plastic. The second solution (with acetic acid) is acidic. If its pH is in the 3 to 4 range it will stain DNA, RNA and acidic carbohydrates (cartilage matrix, mast cells, some mucus etc). This is often what's wanted for paraffin or frozen sections. The choice between staining solutions is based on what you want to see. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ------------------------- > Lina Vieira wrote: > > Helo Everyone, > > In our lab usually, we make the toluidine solution > (for coloration of resin included tissue): > 1g - Borax > 1g - Toluidine Blue > 100 - ml water, > > 1 minute - staining time > > And because, now, I have another protocol for make > the solution, I want ask you about the possibility to > use this solution, and if is it necessary change the > staining time? > > New protocol: > 0.2 g - Toluidine Blue > 0.5 - Acetic acid > Complete, with desionized water, to 100 ml > > > Tanks in advance > > Lina Vieira > From scoop <@t> mail.nih.gov Tue Dec 9 11:29:05 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] PBS Message-ID: Does anyone know why you're supposed to make up 0.2M PBS for perfusion or fixation of slides (to make up neutral buffered formalin or paraformaldehyde) fresh? I understand why it's important to make up the formalin or paraformaldehyde fresh, but what could happen to the PBS (unless something grows in it). Is it really important to make it up fresh? Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From spoulos <@t> saa.ars.usda.gov Tue Dec 9 11:33:08 2003 From: spoulos <@t> saa.ars.usda.gov (Sylvia Poulos) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] unsubscribe Message-ID: Sylvia P. Poulos USDA-ARS-Animal Physiology Research Unit Athens, GA 30605 706-583-8279 706-542-0399 (fax) >>> Carole Fields 12/09/03 8:41 AM >>> THX Marsha, I had a tech turn in her resignation yesterday and it is going to be tough to find a tech for what we pay...it isn't $9... thank goodness. I thought the going rate for a Histo tech however should be at least $19 to start and that is low....and we pay lower than that!!! Any help I can get is appreciated. Carole Fields Lex. Med. Ctn W.Columbia, SC > -----Original Message----- > From: mprice26@juno.com [SMTP:mprice26@juno.com] > Sent: Monday, December 08, 2003 5:09 PM > To: Carole Fields > Subject: RE: [Histonet] Histology Technician Shortage > > > Carole, > I am going to scan and e-mail to everyone tomorrow. I couldn't pull up on > their website either. > > Marsha > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From scoop <@t> mail.nih.gov Tue Dec 9 13:23:34 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] mounting medium for thioflavin T Message-ID: Next question: does anyone know of a fluorescence free resinous (I suppose that means non-aqueous) mounting medium that can be used for Thioflavin T stained slides? I know Apathy's medium works, but is there anything I can buy that's not so messy? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From akwilliams75 <@t> hotmail.com Tue Dec 9 13:50:24 2003 From: akwilliams75 <@t> hotmail.com (kevin williams) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] AFB Positive Patient Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/ef865073/attachment.htm From fmonson <@t> wcupa.edu Tue Dec 9 15:04:49 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] PBS Message-ID: Afternoon Sharon, I routinely make up 10X PBS, autoclave it and store it at room temp to prevent recrystallization. I make up what I estimate that I will need for a month and keep it "for my use ONLY!!!!!" But then, I always check the osmolarity and pH of the final PBS prep, AND I generally filter it just before use. I am finicky, and this quirk may possibly explain why I am considered difficult but reproducible. [Sodium azide is also a good preservative, as long as you are not trying to preserve the life of the system immersed in the PBS.] Cheers, Fred Monson Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Sharon Cooperman [mailto:scoop@mail.nih.gov] Sent: Tuesday, December 09, 2003 12:29 PM To: Histonet Subject: [Histonet] PBS Does anyone know why you're supposed to make up 0.2M PBS for perfusion or fixation of slides (to make up neutral buffered formalin or paraformaldehyde) fresh? I understand why it's important to make up the formalin or paraformaldehyde fresh, but what could happen to the PBS (unless something grows in it). Is it really important to make it up fresh? Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joeamateur <@t> hotmail.com Tue Dec 9 15:11:30 2003 From: joeamateur <@t> hotmail.com (Joe Amateur) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Novice needs help with vacuum infiltration Message-ID: Hi all, I'm a very green, extremely inexperienced novice that has been kind of thrust into learning histology by chance. It's fascinating stuff and I'm eager to learn, but the only training I've been given is two textbooks and the Internet. Thus I need help. We're hand-processing soft (vascular) tissue for H&E in paraffin. We've got a vacuum infiltrator, but no idea how to incorporate it in our protocols (currently using 3 separate baths of molten paraffin at atmospheric pressure at 1 hour each). We know that the infiltration time under vacuum is shorter, but can anyone advise what would work best? Could we put the samples in the infiltrator directly out of xylene, or should we incubate the samples in paraffin first to clear out the xylene? Any help would be deeply, deeply appreciated. --Thanks in advance, Jack England _________________________________________________________________ Don’t worry if your Inbox will max out while you are enjoying the holidays. Get MSN Extra Storage! http://join.msn.com/?PAGE=features/es From leopold <@t> mnsi.net Tue Dec 9 15:30:26 2003 From: leopold <@t> mnsi.net (Derek and/or Lynda Leopold) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Steiner and Steiner method/H. pylori Message-ID: <3FD63EF2.1080107@mnsi.net> Hi list, Would anyone care to comment on the Steiner and Steiner (microwave) method for spirochetes? I susupect my lab's pathologists are interested in it for viewing H. pylori, yet in all the "HP" talk on this list I have yet to hear it mentioned. How does it compare from a tech safety standpoint? As a brand-new tech, I still do crazy things like read the safety warnings on ingredients, and that whole uranyl nitrate radioactive thing is sort of bothering me. We use no protective equipment when doing this stain (which the doctors still call "Warthin Starry", incidentally, but hey---they're making the big bucks....) I'll take anyone's 2 cents... Thanks, Lynda Leopold From pruegg <@t> colobio.com Tue Dec 9 16:11:42 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] IHC on cells on coverslips Message-ID: Please advise how to manage IHC staining for cells grown on coverslips. Patsy From AnthonyH <@t> chw.edu.au Tue Dec 9 16:13:13 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Steiner and Steiner method/H. pylori Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0F3@simba.kids> For HP, the Warthin Starry modifications seem to be the best (?gold standard). Unfortunately many of us find the stain complex and long. Not a good routine stain for most labs. You can replace the uranyl nitrate with Zinc Formalin, Lead nitrate and ferric alum Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Derek and/or Lynda Leopold [mailto:leopold@mnsi.net] Sent: Wednesday, 10 December 2003 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner method/H. pylori Hi list, Would anyone care to comment on the Steiner and Steiner (microwave) method for spirochetes? I susupect my lab's pathologists are interested in it for viewing H. pylori, yet in all the "HP" talk on this list I have yet to hear it mentioned. How does it compare from a tech safety standpoint? As a brand-new tech, I still do crazy things like read the safety warnings on ingredients, and that whole uranyl nitrate radioactive thing is sort of bothering me. We use no protective equipment when doing this stain (which the doctors still call "Warthin Starry", incidentally, but hey---they're making the big bucks....) I'll take anyone's 2 cents... Thanks, Lynda Leopold _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Dec 9 16:21:12 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] RE: [IHCRG] IHC on cells on coverslips Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0F4@simba.kids> Depending on the antibody. Most work after ethanol fixation, though some require air-drying-cold acetone treatment. You could attach a small paper clip to a corner of the coverslip to aid manipulation. Suggest fixation in 95% ethanol, 20min. Block endogenous enzyme (if required), rinse in buffer. You might consider marking the back of the coverslip with a marking pen at this stage (the ink will dissolve in the dehydrating solutions). This will aid in orientation. Proceed with immunohistochemistry. Remember to not let the coverslip dry out. Mount Coverslip with a slide. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, 10 December 2003 9:12 AM To: Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: [IHCRG] IHC on cells on coverslips Please advise how to manage IHC staining for cells grown on coverslips. Patsy ------------------------ Yahoo! Groups Sponsor ---------------------~--> Buy Ink Cartridges or Refill Kits for your HP, Epson, Canon or Lexmark Printer at MyInks.com. Free s/h on orders $50 or more to the US & Canada. http://www.c1tracking.com/l.asp?cid=5511 http://us.click.yahoo.com/mOAaAA/3exGAA/qnsNAA/asSolB/TM ---------------------------------------------------------------------~-> To unsubscribe from this group, send an email to: IHCRG-unsubscribe@egroups.com Your use of Yahoo! Groups is subject to http://docs.yahoo.com/info/terms/ ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From conniegrubaugh <@t> hotmail.com Tue Dec 9 16:34:04 2003 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Steiner and Steiner method/H. pylori Message-ID: I tried to use Zinc Formalin instead of uranyl nitrate and the bugs would not stain. Tried it a couple of times and the Doctors were not happy at all with the results. \\\\ We only use the Steiner when the Doctors think they see bugs but due to a lot of inflammation they need to be more specific. We use the Diff Quick stain for the daily routine. Connie Grubaugh Lab. Med Con Las Vegas Nv >From: Tony Henwood >To: histonet@lists.utsouthwestern.edu >CC: "'Derek and/or Lynda Leopold'" >Subject: RE: [Histonet] Steiner and Steiner method/H. pylori >Date: Wed, 10 Dec 2003 09:13:13 +1100 > >For HP, the Warthin Starry modifications seem to be the best (?gold >standard). >Unfortunately many of us find the stain complex and long. Not a good >routine >stain for most labs. >You can replace the uranyl nitrate with Zinc Formalin, Lead nitrate and >ferric alum > >Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) >Laboratory Manager >The Children's Hospital at Westmead, >Locked Bag 4001, Westmead, 2145, AUSTRALIA. >Tel: 612 9845 3306 >Fax: 612 9845 3318 > >http://www.histosearch.com/homepages/TonyHenwood/default.html >http://us.geocities.com/tonyhenwoodau/index.html > > >-----Original Message----- >From: Derek and/or Lynda Leopold [mailto:leopold@mnsi.net] >Sent: Wednesday, 10 December 2003 8:30 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Steiner and Steiner method/H. pylori > > >Hi list, >Would anyone care to comment on the Steiner and Steiner (microwave) >method for spirochetes? I susupect my lab's pathologists are interested >in it for viewing H. pylori, yet in all the "HP" talk on this list I >have yet to hear it mentioned. How does it compare from a tech safety >standpoint? As a brand-new tech, I still do crazy things like read the >safety warnings on ingredients, and that whole uranyl nitrate >radioactive thing is sort of bothering me. We use no protective >equipment when doing this stain (which the doctors still call "Warthin >Starry", incidentally, but hey---they're making the big bucks....) >I'll take anyone's 2 cents... >Thanks, >Lynda Leopold > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >********************************************************************** >This email and any files transmitted with it are confidential and >intended solely for the use of the individual or entity to whom they >are addressed. If you are not the intended recipient, please >delete it and notify the sender. > >Views expressed in this message and any attachments are those >of the individual sender, and are not necessarily the views of The >Children's Hospital at Westmead > >This footnote also confirms that this email message has been >virus scanned and although no computer viruses were detected, >the Childrens Hospital at Westmead accepts no liability for any >consequential damage resulting from email containing computer >viruses. >********************************************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Don’t worry if your Inbox will max out while you are enjoying the holidays. Get MSN Extra Storage! http://join.msn.com/?PAGE=features/es From SJones <@t> cvm.tamu.edu Tue Dec 9 16:35:29 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Novice needs help with vacuum infiltration Message-ID: Hi Joe, Oh, the heady days of processing by hand and Uri Gagarin. (a little Hunt for Red October lingo there). How many baths does your vacuum infiltrator have? I assume this is not an autoprocessor, but just for the paraffins only. You can put the samples in directly from xylene. Can you change the paraffin out with new baths if it only holds one bath? How long are you leaving the tissues in paraffin now? How big are the samples? Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Joe Amateur" 12/9/2003 3:11:30 PM >>> Hi all, I'm a very green, extremely inexperienced novice that has been kind of thrust into learning histology by chance. It's fascinating stuff and I'm eager to learn, but the only training I've been given is two textbooks and the Internet. Thus I need help. We're hand-processing soft (vascular) tissue for H&E in paraffin. We've got a vacuum infiltrator, but no idea how to incorporate it in our protocols (currently using 3 separate baths of molten paraffin at atmospheric pressure at 1 hour each). We know that the infiltration time under vacuum is shorter, but can anyone advise what would work best? Could we put the samples in the infiltrator directly out of xylene, or should we incubate the samples in paraffin first to clear out the xylene? Any help would be deeply, deeply appreciated. --Thanks in advance, Jack England _________________________________________________________________ Don't worry if your Inbox will max out while you are enjoying the holidays. Get MSN Extra Storage! http://join.msn.com/?PAGE=features/es _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From powell_sa <@t> Mercer.EDU Tue Dec 9 16:43:10 2003 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] IHC on cells on coverslips References: Message-ID: <001201c3bea5$d25d3170$a6f2acd1@powellsa1> Patsy, I have done IHC on coverslips using the batch method in short Coplin jars made for coverslips. I have seen them in several vendor catalogs. Also I purchased a stain set up from Thermo Electron, when it was Shandon Lipshaw, with 6 glass jars that holds about 8-10 oz in a stainless steel rack with hinged cover. This had a small slide rack that could hold the larger coverslips that may work as well. Shirley ----- Original Message ----- From: "Patsy Ruegg" To: "Histonet@Pathology. Swmed. Edu" Cc: "Ihcrg@Yahoogroups. Com" Sent: Tuesday, December 09, 2003 5:11 PM Subject: [Histonet] IHC on cells on coverslips > Please advise how to manage IHC staining for cells grown on coverslips. > Patsy > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mghoddusi <@t> cmri.usyd.edu.au Tue Dec 9 16:50:48 2003 From: mghoddusi <@t> cmri.usyd.edu.au (Majid Ghoddusi) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Novice needs help with vacuum infiltration Message-ID: Hi All, While on this subject, I would appreciate it if you could share your entire protocol for hand processing with me. Someone in our lab has just started doing it for his embryo/fetus/muscle samples and he is not having much fun :) Regards, Majid Ghoddusi ........................................................... Majid Ghoddusi DVM, PhD Senior Microscopist Muscle Development Unit Children's Medical Research Institute Locked Bag 23, Wentworthville NSW 2145 .............................................................. -----Original Message----- From: Sarah Jones [mailto:SJones@cvm.tamu.edu] Sent: Wednesday, 10 December 2003 9:35 AM To: joeamateur@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Novice needs help with vacuum infiltration Hi Joe, Oh, the heady days of processing by hand and Uri Gagarin. (a little Hunt for Red October lingo there). How many baths does your vacuum infiltrator have? I assume this is not an autoprocessor, but just for the paraffins only. You can put the samples in directly from xylene. Can you change the paraffin out with new baths if it only holds one bath? How long are you leaving the tissues in paraffin now? How big are the samples? Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 >>> "Joe Amateur" 12/9/2003 3:11:30 PM >>> Hi all, I'm a very green, extremely inexperienced novice that has been kind of thrust into learning histology by chance. It's fascinating stuff and I'm eager to learn, but the only training I've been given is two textbooks and the Internet. Thus I need help. We're hand-processing soft (vascular) tissue for H&E in paraffin. We've got a vacuum infiltrator, but no idea how to incorporate it in our protocols (currently using 3 separate baths of molten paraffin at atmospheric pressure at 1 hour each). We know that the infiltration time under vacuum is shorter, but can anyone advise what would work best? Could we put the samples in the infiltrator directly out of xylene, or should we incubate the samples in paraffin first to clear out the xylene? Any help would be deeply, deeply appreciated. --Thanks in advance, Jack England _________________________________________________________________ Don't worry if your Inbox will max out while you are enjoying the holidays. Get MSN Extra Storage! http://join.msn.com/?PAGE=features/es _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joeamateur <@t> hotmail.com Tue Dec 9 17:00:19 2003 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] More specific information from the rookie Message-ID: Hi again all, So far you've all provided a huge help already, but here's a little more info that may help. The paraffin that we're using is TBS PolyFin, mp 55 deg C. The samples that we're infiltrating are vascular tissue; tubular samples approx. 1cm long with a wall thickness of approx. 0.5-1mm. The books I've been studying from are: --Bancroft, J and Gamble, M ed. (2002): Theory and Practice of Histological Techniques, 5th ed. Churchill , ISBN: 0-443-06435-0 --Prophet, E. et. al, ed. (1994): Laboratory Methods in Histotechnology. American Registry of Pathology, ISBN 1-881041-00-X I'm kind of embarrassed to say it, but we don't have an oven (PI decided current equipment should work for now), so our 3 paraffin baths are in an incubator set at 55 C. I don't know whether having our paraffin baths at the melting point of the paraffin is a bad thing or a good thing, but my suspicion is that it is not good, so I'm lobbying for an oven. The vacuum infiltrator is one big tub set into our embedding center, so I think we'd have a hard time changing out the paraffin multiple times. I was toying with the idea of putting 3-4 small jars inside it and using those, since the samples are so small. Thanks again for all the help so far--you've been a lot friendlier to an obviously inexperienced (and undertrained) novice than I was expecting. Thank you so much!! --Regards, Jack England _________________________________________________________________ Get holiday tips for festive fun. http://special.msn.com/network/happyholidays.armx From RFail <@t> Charleston.net Tue Dec 9 16:41:00 2003 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Steiner and Steiner method/H. pylori In-Reply-To: <3FD63EF2.1080107@mnsi.net> Message-ID: <000801c3bea5$874c4630$9e11a6a5@rena> Lynda, The microwave method is fairly easy, we get consistently good results with our method. Rena Fail -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Derek and/or Lynda Leopold Sent: Tuesday, December 09, 2003 4:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner method/H. pylori Hi list, Would anyone care to comment on the Steiner and Steiner (microwave) method for spirochetes? I susupect my lab's pathologists are interested in it for viewing H. pylori, yet in all the "HP" talk on this list I have yet to hear it mentioned. How does it compare from a tech safety standpoint? As a brand-new tech, I still do crazy things like read the safety warnings on ingredients, and that whole uranyl nitrate radioactive thing is sort of bothering me. We use no protective equipment when doing this stain (which the doctors still call "Warthin Starry", incidentally, but hey---they're making the big bucks....) I'll take anyone's 2 cents... Thanks, Lynda Leopold _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joeamateur <@t> hotmail.com Tue Dec 9 17:34:20 2003 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Rookie's manual-prep/H&E protocol Message-ID: Hi Majid, Here's our protocol: Fix: Place tissue in 1xDPBS for a few hours at 4 degrees C. (From what I've read, I'm wary of how well this works, so advice would be welcomed ...I prefer 70%EtOH overnight) Dehydrate: 70% EtOH --1hr 95% EtOh --1hr 95% EtOh --1hr 3x 100% EtOH--1 hr each Clear: Xylene overnight Infiltration: 3 x TBS paraffin, 1 hour each at atmospheric pressure (this is one part I'm trying to refine) Embed, and allow to harden overnight. Section at 7um and apply to slides; allow to dry overnight on slide warmer. Rehydrate: Xylene 1: 3 min Xylene 2: 2 min Xylene/100%EtOH: 2 min 100% EtOH: 2 min 95% EtOH: 2 min 80% EtOH: 1 min 70% EtOH: 1 min 50% EtOH: 1 min di water: 3 min (per another thread, I consider water deionized at or above 18 megaohm-cm) Stain in Gill's Hematoxylin 2 for 2-4 min (we're still working on optimizing this one) Rinse: Running tap water: 1 min Tap water: 1 min di water: 3-5 min Counterstain in eosin (2.5%--5% diluted 1:2) with a quick dip. Dehydration: 95% EtOH: 2 min 95% EtOH: 2 min 100% EtOH: 2 min Xylene/100%EtOH: 2 min Xylene 2: 2 min Xylene 1: 3 min Apply mounting media to slide and cover. Let dry in fume hood for a few minutes before examining. Hope this helps! --Regards, Jack England _________________________________________________________________ Take advantage of our best MSN Dial-up offer of the year — six months @$9.95/month. Sign up now! http://join.msn.com/?page=dept/dialup From RFail <@t> Charleston.net Tue Dec 9 19:38:03 2003 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] AFB Positive Patient In-Reply-To: Message-ID: <000101c3bebe$41c4b520$a210a6a5@rena> In order to prevent cross- contamination, We don't even hydrate or dehydrate the acid fast controls and pt tissue through the same solutions. try google images for good pics.or the Nov 2000 issue of Histologic. Rena Fail -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of kevin williams Sent: Tuesday, December 09, 2003 2:50 PM To: histonet@pathology.swmed.edu Subject: [Histonet] AFB Positive Patient Would just like to thank the very kind person who gave me the AFB control that my Pathologist is lording over, we had an immuno suppressed patient biopsy and in one of the sections there is a great vacule with an abundance of AFB. We are now trying to work out if anything went wrong, has anyone come across any unusual situations where a false positive could be created? The control and tissue where on the same slide, but a good distance apart, the bacteria in the patient are in the same place on two consecutive sections. Also if you know of any sites with good pictures of AFB positive tissue I would be interested in looking at these. Yours faithfully: A. Kevin Williams _____ Don't worry if your Inbox will max out while you are enjoying the holidays. Get MSN Extra Storage! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/5ec9b704/attachment.htm From portera203 <@t> yahoo.com Tue Dec 9 22:03:20 2003 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] need help finding a Luna Stain procedure In-Reply-To: <4.3.2.7.2.20031208143021.00cdeea8@algranth.inbox.email.arizona.edu> Message-ID: <20031210040320.33849.qmail@web40912.mail.yahoo.com> Andi - We do a Luna's stain for eosinaphils in our lab. It is from the most recent armed forces publication. It involves using Biebrich Scarlet and Weigerts Hematoxylin. I am out of the office until Thursday. I would be happy to post something for you then or email you off list. Let me know. Andrea Grantham wrote: Happy Monday! I'm looking for the procedure to do a Luna stain. We have some skin samples probably with sun damage and we want to look at the grenz zone in them. A Luna stain for elastin was discussed in April, 03 on histonet but a search of the archives did not turn up the procedure. I found a Gomori Aldehyde Fuchsin/Trichrome by Lee Luna and Darryl Luna in an old HistoLogic but I'm not sure that this is the same stain. Thanks. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Amy S.Porter, HT(ASCP) Michigan State University Department of Physiology Division of Human Pathology College of Human Medicine portera203@yahoo.com --------------------------------- Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031209/5f5abd18/attachment.htm From louise_renton <@t> hotmail.com Wed Dec 10 02:08:09 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] AFB Positive Patient Message-ID: Kevin, Hi Kevin, I have seen a slide of lymph node from a patient with a severely immunocompromised state who has such a superabundance of AFB that the section was visibly pink to the naked eye. Needless to say, these sections were NOT used as controls for routine work. Interestingly, when PCR typing was performed, the bugs turned out to M avis and not MTb as initially suspected. This is completely anecdotal, but I have heard of various mycobacteriae lurking in tap water, that show up on sections washed in that water, causing "false positives". Has anyone else anything to offer on this possible urban legend? best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "kevin williams" To: histonet@pathology.swmed.edu Subject: [Histonet] AFB Positive Patient Date: Tue, 09 Dec 2003 19:50:24 +0000 _________________________________________________________________ Chat, flirt, play and share online with Messenger 6.1 - it's FREE! http://messenger.msn.co.za/ -------------- next part -------------- An embedded message was scrubbed... From: "kevin williams" Subject: [Histonet] AFB Positive Patient Date: Tue, 09 Dec 2003 19:50:24 +0000 Size: 4443 Url: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/00607d83/attachment.eml From nick.kirk3 <@t> btopenworld.com Wed Dec 10 02:40:15 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] AFB Positive Patient In-Reply-To: Message-ID: Louise I too have heard of the urban myth of Mycobacteria lurking in tap water, but personally have never actually seen it and we do a reasonable number of ZNs on a regular basis., nor do I know of anyone who can prove that they have actually seen it as well The point made about not using controls with an abundance of bacteria is well made, as the vast majority of positive cases will only have a few bacteria in them, so you need to hunt around a bit for them, so a control with a few scattered bacteria is more preferable. As for the point raised by another Histonetter about using different alcohols, well personally I think that is maybe going a bit too far. Mycobacterium, as far as I'm aware, don't thrive in alcohol so the risk of contamination is very small, plus if the sections are taken to water before staining, they are washed so I would have thought that would further reduce the risk of contamination to almost zero. We dewax all our special stains slides on our Leica ST5020 staining machine where the reagents are regularly changed, plus they are washed in running tap water. To my mind the biggest risk of contamination comes from the floating out water bath following sectioning. If the water isn't changed on a regular basis you can get all manner of "creatures" growing in them. If there is going to be any cross-contamination, that's where it will come from. Tot siens en geniet jou dag Louise Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of louise renton Sent: 10 December 2003 08:08 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AFB Positive Patient Kevin, Hi Kevin, I have seen a slide of lymph node from a patient with a severely immunocompromised state who has such a superabundance of AFB that the section was visibly pink to the naked eye. Needless to say, these sections were NOT used as controls for routine work. Interestingly, when PCR typing was performed, the bugs turned out to M avis and not MTb as initially suspected. This is completely anecdotal, but I have heard of various mycobacteriae lurking in tap water, that show up on sections washed in that water, causing "false positives". Has anyone else anything to offer on this possible urban legend? best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "kevin williams" To: histonet@pathology.swmed.edu Subject: [Histonet] AFB Positive Patient Date: Tue, 09 Dec 2003 19:50:24 +0000 _________________________________________________________________ Chat, flirt, play and share online with Messenger 6.1 - it's FREE! http://messenger.msn.co.za/ From t-sherman <@t> comcast.net Wed Dec 10 04:25:33 2003 From: t-sherman <@t> comcast.net (Todd Sherman) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Re: Change from digest to individual emails (Bill Blank) In-Reply-To: <20031209180001.17579.50652.Mailman@swlx167.swmed.edu> References: <20031209180001.17579.50652.Mailman@swlx167.swmed.edu> Message-ID: <3FD6F49D.4070701@comcast.net> -----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 Hello Bill, When you signed up, you should have received a notice of the methods for modifying your newsgroup settings. To review/modify your settings, browse to: http://lists.utsouthwestern.edu/mailman/options/histonet/name@domain.xyz where name = the email account name you use to receive the digest, and domain.xyz = your ISP domain name. Hope this helps, Todd histonet-request@lists.utsouthwestern.edu wrote: | | Today's Topics: | | 2. Change from digest to individual emails (Bill Blank) | --__--__-- | | Message: 2 | Date: Mon, 8 Dec 2003 13:09:54 -0600 | To: histonet@lists.utsouthwestern.edu | From: Bill Blank | Subject: [Histonet] Change from digest to individual emails | | How do I change from digest to individual emails on this list? | | | TIA, | | Bill | | --__--__-- -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.2.3 (MingW32) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org iD8DBQE/1vSKEmHXdrslGRcRAkhsAKDEdyIK94cyL545RFyii+P5CRKYCwCgu9CP LsO+iKUBbZhuPfm/4i4V/8E= =NqTx -----END PGP SIGNATURE----- From stevemachinuk <@t> yahoo.co.uk Wed Dec 10 04:39:07 2003 From: stevemachinuk <@t> yahoo.co.uk (=?iso-8859-1?q?Steve=20Machin=20UK?=) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Sialyl-Tn antibody Message-ID: <20031210103907.42131.qmail@web25110.mail.ukl.yahoo.com> Does anyone know where I could buy, in the UK, an antigen to Sialyl-Tn antigen for use with paraffin sections? ________________________________________________________________________ BT Yahoo! Broadband - Save ?80 when you order online today. Hurry! Offer ends 21st December 2003. The way the internet was meant to be. http://uk.rd.yahoo.com/evt=21064/*http://btyahoo.yahoo.co.uk From jqb7 <@t> cdc.gov Wed Dec 10 05:51:46 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Steiner and Steiner method/H. pylori Message-ID: Lynda: We do the Steiner and Steiner regularly. I used to have issues with our waste disposal group here about the uranyl nitrate until I showed them the product insert we received from PolyScientific, which is where we purchase the product. It listed all the things that were more radioactive than the uranyl nitrate solution such as a compass and watch battery. I would be glad to send you a copy if it would ease your mind. As far as the procedure goes, when I have time I use the waterbath method for the silver as I get less precipitate. I do use the microwave for the sensitization step. It's a nice, clean procedure when done carefully and gently. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Derek and/or Lynda Leopold Sent: Tuesday, December 09, 2003 4:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steiner and Steiner method/H. pylori Hi list, Would anyone care to comment on the Steiner and Steiner (microwave) method for spirochetes? I susupect my lab's pathologists are interested in it for viewing H. pylori, yet in all the "HP" talk on this list I have yet to hear it mentioned. How does it compare from a tech safety standpoint? As a brand-new tech, I still do crazy things like read the safety warnings on ingredients, and that whole uranyl nitrate radioactive thing is sort of bothering me. We use no protective equipment when doing this stain (which the doctors still call "Warthin Starry", incidentally, but hey---they're making the big bucks....) I'll take anyone's 2 cents... Thanks, Lynda Leopold _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adelaur <@t> nimr.mrc.ac.uk Wed Dec 10 13:53:05 2003 From: adelaur <@t> nimr.mrc.ac.uk (April DeLaurier) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Alcian yellow stain for cartilage Message-ID: Hi, Has anyone had any experience using alcian yellow as a stain for cartilage? Thanks, April -- April DeLaurier, PhD Division of Developmental Biology National Institute for Medical Research The Ridgeway, Mill Hill London NW7 1AA United Kingdom Tel: +44 208 959 3666 ext. 2095 Fax: +44 208 816 4477 E-mail: adelaur@nimr.mrc.ac.uk From Myri37 <@t> aol.com Wed Dec 10 06:05:16 2003 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] SEM for collagen fibers Message-ID: <11AFF294.0212AAEE.0005167B@aol.com> hello dear histonetters first many thanks for your last precious help I have few questions about identification of collagen fibers in resconstructed tissue Do you think that fixation of small tissue with glutarald?hyde for three days can avoid proper stainning of collagen fibers with trichrome stains, should i change the fixative to paraformald?hyde for instance or reduce time of fixation ? Do you know if it's possible to do SEM observations on sections which have been previously embedded in methylmetacrylate or paraffine ? the issue is as well to observe collagen fibers and their orientation i have no experience to doing that, does someone have a protocol ? thank you so much for anyhelp Myriam baali Natural implant 163, av de luminy marseille, france tel: 04 91 80 41 89 From tpschaer <@t> vet.upenn.edu Wed Dec 10 08:32:05 2003 From: tpschaer <@t> vet.upenn.edu (Tom Schaer) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] polymerization problems Message-ID: <00d401c3bf2a$62ab7000$38595b82@vet.upenn.edu> Dear Histonetters: I am going to try again, realizing that not many have vast experience with embedding large specimen with technovit 9100 yet. Maybe someone may be able to share some pointers for a non-profit lab? Essentially, we fail to successfully polymerize med fem ovine condyles in approx 100ml jars. Tried at -15C with no change in solution consistency for a week; then brought it up to -2C still no change over three days. Jars were "vacuumed" and set in refrigerator / freezer. I am at the of my rope of troubleshooting. Thanks for any pointers. Tom ------------------------------------------ Thomas P. Schaer, VMD Assist. Prof. BIOMED, Drexel University Comparative Orthopaedic Research & Tissue Engineering Department of Clinical Studies University of Pennsylvania New Bolton Center 382 West Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office) tel. 610-444-5800 (x6131 lab) fax. 610-925-8100 tpschaer@mail.vet.upenn.edu -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/d9d44cb5/attachment.htm From haldana <@t> unimoron.edu.ar Wed Dec 10 09:01:28 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] SEM for collagen fibers References: <11AFF294.0212AAEE.0005167B@aol.com> Message-ID: <000d01c3bf2e$7e6a4380$7504a8c0@um.edu> You have a lot of methods I send two: 1- Tissues was fixed by the immersion procedure (gluta or parafor). It was dehydrated in a graded series of ethanol, and fractured with frozen ethanol. The samples were dried by the critical point method with carbon dioxide. Samples were mounted on aluminum stubs, sputtercoated with gold, and examined in a Jeol JSM-15 scanning electron microscope. 2- Tissues were transferred to phosphate buffer (pH 7.4) for 2-3 d to remove excess of glutaraldehyde and soften, before dehydrating throught alcohols, clearing in amyl acetate and embedding. Tissues were sectioned at 5 ?m until some particualr feature (such as the gland duct) was reached. The final section was then stained with H-E to act as a reference and unsectioned tissue reprocessed for SEM. Wax was removed by trimming, by heating at 56?C and finally by tranferring to xylene at 37?C, receiving 4-5 changes of xylene over 24-36 h. Tissues were placed in 2 washes of absolute alcohol, critical point dired, mounted and coated as a usuall. (J. McGadey et al. J Anat (1992) 180. 127-136.) 3- we do the two techniques with excelllent results. Also we make a modification in the technique two. When I make the bloks in parafin I fracture the block with my hands or using the "floor shock" and then make the reprocessing to SEM. Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: To: Sent: Wednesday, December 10, 2003 9:05 AM Subject: [Histonet] SEM for collagen fibers > hello dear histonetters > first many thanks for your last precious help > I have few questions about identification of collagen fibers in resconstructed tissue > Do you think that fixation of small tissue with glutarald?hyde for three days can avoid proper stainning of collagen fibers with trichrome stains, should i change the fixative to paraformald?hyde for instance or reduce time of fixation ? > Do you know if it's possible to do SEM observations on sections which have been previously embedded in methylmetacrylate or paraffine ? > the issue is as well to observe collagen fibers and their orientation > i have no experience to doing that, does someone have a protocol ? > thank you so much for anyhelp > > > Myriam baali > Natural implant > 163, av de luminy > marseille, france > tel: 04 91 80 41 89 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ian.montgomery <@t> bio.gla.ac.uk Wed Dec 10 09:16:18 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] DAB kits. Message-ID: <6.0.0.22.2.20031210151034.02d15b70@udcf.gla.ac.uk> I find that my DAB kits are still at least 2/3 full when they reach their expiry date. Is it possible to aliquot the components, freeze then thaw when needed. Or, can I simply freeze the kit and thaw as needed. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From mcauliff <@t> umdnj.edu Wed Dec 10 09:38:29 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] IHC on cells on coverslips In-Reply-To: <001201c3bea5$d25d3170$a6f2acd1@powellsa1> References: <001201c3bea5$d25d3170$a6f2acd1@powellsa1> Message-ID: <3FD73DF5.9060605@umdnj.edu> The "short Coplin jars" are called Columbia staining jars. Protozologists use them to save on expensive reagents, at least they did when I was "growing up" in a Biology dept. Geoff Shirley Powell wrote: >Patsy, > >I have done IHC on coverslips using the batch method in short Coplin jars >made for coverslips. I have seen them in several vendor catalogs. Also I >purchased a stain set up from Thermo Electron, when it was Shandon Lipshaw, >with 6 glass jars that holds about 8-10 oz in a stainless steel rack with >hinged cover. This had a small slide rack that could hold the larger >coverslips that may work as well. > >Shirley > >----- Original Message ----- >From: "Patsy Ruegg" >To: "Histonet@Pathology. Swmed. Edu" >Cc: "Ihcrg@Yahoogroups. Com" >Sent: Tuesday, December 09, 2003 5:11 PM >Subject: [Histonet] IHC on cells on coverslips > > > > >>Please advise how to manage IHC staining for cells grown on coverslips. >>Patsy >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From ian.montgomery <@t> bio.gla.ac.uk Wed Dec 10 10:03:45 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] IHC on coverglasses. Message-ID: <6.0.0.22.2.20031210155502.02d12ac0@udcf.gla.ac.uk> Put a small spot of silicone grease in the middle of a glass slide. Drain the coverglass then touch against the grease, cells up, press gently then ring with a pen. Handle with care during the technique and press again if you think it's needed. When you're finished simply slip off and mount on a slide as usual. If aqueous I clean the excess grease off after the mountant has set. If you dehydrate the grease dissolves in the clearing agent. Vaseline works but silicone grease gives a better attachment during all the rinsing steps. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk From vbaker60 <@t> yahoo.com Wed Dec 10 10:20:57 2003 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Cox-2 antibody from Santa Cruz Message-ID: <20031210162057.8217.qmail@web12101.mail.yahoo.com> Hi, Is anyone currently using the Cox-2 (goat polyclonal)from Santa Cruz? Up until my last two runs I wasn't having any problems with it. My concentration is 1:1000, I'm using EDTA as HIER in a microwave (1200watt, two 3 minute cycles pl 7; boil/simmer is approximately 30 seconds each). I'm using a Vector universal ABC detection kit with Nova Red chromogen for visualization. The tissue is human cervical samples, and I've had good results from them in the past. I have replaced the antibody and the chromogen. The detection kit is only 3 months old. Any input would be very much appreciated. Vikki Baker Institute for Cancer Prevention Valhalla, NY 10595 __________________________________ Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing. http://photos.yahoo.com/ From clarkda <@t> ohsu.edu Wed Dec 10 10:53:16 2003 From: clarkda <@t> ohsu.edu (David Clark) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Gallayas Stain Message-ID: Hi, Does anyone have a good reliable method for the Gallayas Technique for Neurofibrillary Changes that they would be willing to share with me? Thank-you. David Clark Neuropathology Oregon Health & Science Univ. Portland, Or From clarkda <@t> ohsu.edu Wed Dec 10 10:59:45 2003 From: clarkda <@t> ohsu.edu (David Clark) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Immuno procedure for PrP with Hydrolytic Autoclaving Message-ID: Hello, I'm about to embark on a new adventure. I'm wanting to stain some CJD material using a PrP antibody. The procedure requires hydrolytic autoclaving. Has anybody had any experience with this procedure? Any information would be greatly appreciated. Thank-you. David Clark Neuropathology OHSU Portland, Or From Bonnie.P.Whitaker <@t> uth.tmc.edu Wed Dec 10 10:42:09 2003 From: Bonnie.P.Whitaker <@t> uth.tmc.edu (Bonnie P Whitaker) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Cox-2 antibody from Santa Cruz In-Reply-To: <20031210162057.8217.qmail@web12101.mail.yahoo.com> Message-ID: Are you getting any staining? (Please describe exactly what the problem is.) Thanks, Bonnie -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Victoria Baker Sent: Wednesday, December 10, 2003 10:21 AM To: HistoNet Server Subject: [Histonet] Cox-2 antibody from Santa Cruz Hi, Is anyone currently using the Cox-2 (goat polyclonal)from Santa Cruz? Up until my last two runs I wasn't having any problems with it. My concentration is 1:1000, I'm using EDTA as HIER in a microwave (1200watt, two 3 minute cycles pl 7; boil/simmer is approximately 30 seconds each). I'm using a Vector universal ABC detection kit with Nova Red chromogen for visualization. The tissue is human cervical samples, and I've had good results from them in the past. I have replaced the antibody and the chromogen. The detection kit is only 3 months old. Any input would be very much appreciated. Vikki Baker Institute for Cancer Prevention Valhalla, NY 10595 __________________________________ Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing. http://photos.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jfish <@t> gladstone.ucsf.edu Wed Dec 10 11:26:32 2003 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Gallayas Stain In-Reply-To: References: Message-ID: Dear David, I have used the protocol in Freida Carson's book, HISTOTECHNOLOGY: A SELF-INSTRUCTIONAL TEXT, 2nd edition. It worked very well! Jo Dee Fish ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 From siksik03 <@t> comcast.net Wed Dec 10 11:24:52 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Steiner and Steiner method/H. pylori In-Reply-To: <3FD63EF2.1080107@mnsi.net> References: <3FD63EF2.1080107@mnsi.net> Message-ID: Hi HistoNetters There certainly is a Wrathin Starry microwave method to demonstrate spirochetes, and it does not involve anything radioactive. Here is Joyce Moore's version, as it was taught to me: Warthin Starry Techniques for Spirochetes Fixation: Formalin Technique: Sections cut at 4 microns Control: Tissue containing Spirochetes Solutions: Acidulated WaterAcidulate 1 liter distilled water with 0.1 g citric acid until pH of 3.8-4.4 is reached. A pH of 4.0 is ideal for staining spirochetes. For demonstrating Donovan Bodies of granuloma inguinale, a pH of 3.6 is recommended. 2% Silver Nitrate Solution (developer)Silver nitrate C.P. crystals 0.5 gAcidulated water 25.0 ml 1% Silver Nitrate Solution (impregnation)Silver nitrate C.P. crystals 0.5 gAcidulated water 50.0 ml 0.5% Hydroquinone SolutionHydroquinone crystals, photographic quality 0.35 gAcidulated water 25.0 ml 5% Gelatin SolutionGelatin 1.5 gAcidulated water 25.0 ml Staining Procedure 1. Attach slides to automatic stainer, deparaffinize, hydrate to water. 2. Rinse in distilled water, 2 changes. 3. Place slides in 1% silver nitrate solution for 45 seconds in microwave. Let stand for 1 minute at room temperature. (Note: alternatively, in a laboratory microwave, heat at 80% power, 60 C for 5 minutes, no standing time required). 4. Preheat for 45 seconds in microwave in separate flasks: 2% silver nitrate, 5% gelatin, 0.15% hydroquinone. Preheat empty flask in microwave with these. 5. Mix developer solution in order given: Use warm empty flask: 2% silver nitrate 1.5 ml5% gelatin 3.75 ml0.15% hydroqinone 2.0 ml 6. When step 5 is completed, remove slides from silver solution. Do not rinse. Place slides horizontally on a slide rack and cover with developer. Allow sections to develop until they are light yellow to golden brown, approximately 1 minute or less. (Note: developing step can also be carried out in a laboratory microwave at 80% power set for 60 C for 20 seconds). 7. Rinse thoroughly in 50 ml tap water which is preheated to approximately 56 C in the microwave at 450W for 45 seconds. 8. Rinse in distilled water. 9. Attach to automatic stainer, dehydrate and clear. Mount in a xylene soluble mounting medium. Results: Spirochetes - blackBackground - pale yellow to light brown Adapted for the Jefferson Regional Medical Center Histo-Path Laboratory best regards, Steven Slap At 4:30 PM -0500 12/9/03, Derek and/or Lynda Leopold wrote: >Hi list, >Would anyone care to comment on the Steiner and Steiner (microwave) >method for spirochetes? I susupect my lab's pathologists are >interested in it for viewing H. pylori, yet in all the "HP" talk on >this list I have yet to hear it mentioned. How does it compare from >a tech safety standpoint? As a brand-new tech, I still do crazy >things like read the safety warnings on ingredients, and that whole >uranyl nitrate radioactive thing is sort of bothering me. We use no >protective equipment when doing this stain (which the doctors still >call "Warthin Starry", incidentally, but hey---they're making the >big bucks....) >I'll take anyone's 2 cents... >Thanks, >Lynda Leopold > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/c988ddfe/attachment.htm From jfish <@t> gladstone.ucsf.edu Wed Dec 10 11:31:05 2003 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] Gallayas Stain In-Reply-To: References: Message-ID: Sorry David and everyone, As soon as I hit the send button I realized my mistake! The Gallyas method I have used is in Bancroft, JD: THEORY AND PRACTICE OF HISTOLOGICAL TECHNIQUES, New York: Churchill Livingstone, 1996 . Please, don't everyone send me scolding emails at once, I was using my old memory as a reference, rather than actually taking down the book and looking it up. (At least I remember using this staining method!). Take care everyone and Happy Holidays! Jo Dee ********************************************************************** ********** Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 695-3720 Fax: (415) 285-5632 E-mail: jfish@gladstone.ucsf.edu Mailing address: Gladstone Institutes P.O. Box 419100 San Francisco, CA 94141-9100 From bryand <@t> netbistro.com Wed Dec 10 12:09:41 2003 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:22:19 2005 Subject: [Histonet] AFB organisms in tap water Message-ID: <008301c3bf48$c9eb56e0$9870c2cf@bryand> Mycobacteria lurking in taps is not an urban legend, it is real. I have had it happen. We traced some ZN positive bacteria to the crud (a technical term) on the inside of a vacuum trap at our special stains bench. The minerals in the water and, I presume, the materials in the air were enough to grow plates of the organisms. Fragments of these plates broke off and adhered to sections giving false positives. The organisms were thicker and longer than TB and strongly beaded, but who knows what others could look like. After that I regularly cleaned the inside of all the taps in the lab with brushes, and it never happened again. Bryan Llewellyn ----- Original Message ----- From: "louise renton" To: Sent: Wednesday, December 10, 2003 12:08 AM Subject: RE: [Histonet] AFB Positive Patient > Kevin, > Hi Kevin, > > I have seen a slide of lymph node from a patient with a severely > immunocompromised state who has such a superabundance of AFB that the > section was visibly pink to the naked eye. Needless to say, these sections > were NOT used as controls for routine work. Interestingly, when PCR typing > was performed, the bugs turned out to M avis and not MTb as initially > suspected. > > This is completely anecdotal, but I have heard of various mycobacteriae > lurking in tap water, that show up on sections washed in that water, causing > "false positives". Has anyone else anything to offer on this possible urban > legend? > > best regards > > > > Louise Renton > Bone Research Unit > MRC > Johannesburg > South Africa > Tel & fax +27 11 717 2298 > "Time flies like an arrow, fruit flies like a banana" > > > > > > ----Original Message Follows---- > From: "kevin williams" > To: histonet@pathology.swmed.edu > Subject: [Histonet] AFB Positive Patient > Date: Tue, 09 Dec 2003 19:50:24 +0000 > > _________________________________________________________________ > Chat, flirt, play and share online with Messenger 6.1 - it's FREE! > http://messenger.msn.co.za/ > From tflore <@t> lsuhsc.edu Wed Dec 10 12:35:17 2003 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Renal biopsy charges/procedures Message-ID: Martha, sorry, I have been on vacation and just did return. Please go to our WEB site at http://www.lsuhsc.edu/no/schools/ms/departments/pathology/pathist/dx_ home.html I am checking to see if our charges are in our web site and if not, we are placing them in there, but here they are for they time being. For a biopsy to be processed STAT the requisition form must be labeled STAT. Remember that the cut off time for STATS is 11:00am. Specimens that arrive before 2pm will have 24 hour turn around time. Specimens that arrive after 2pm will have a 48 hour turn around time. Immunofluorescent (IF); High Resolution Light Microscopy (HRLM); Transmission Electron Microscopy (TEM) Level IV and Level V HRLM only CPT Code 88349 (Routine) $400.00 CPT Code 88349 (STAT) $500.00 CPT Code 88349 (Unsatisfactory) $160.00 CPT Code 88346 (Routine) Each antibody is $50.00 Performed are: IgG, IgM, IgA, C3, C1q, (C4d for transplants on request), Fibrinogen, Kappa and Lambda. CPT Code 88346 (Unsatisfactory) $50.00 CPT Code 88348 (Routine) $800.00 Price includes HRLM, TEM and immunofluorescent studies. CPT Code 88348 (STAT )$1000.00 -----Original Message----- From: Martha Ward [mailto:mward@wfubmc.edu] Sent: Monday, December 01, 2003 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Renal biopsy charges/procedures We are currently looking at our charges and procedures for renal biopsies and my manager wanted me to see if anyone on the Histonet would be willing to share what procedures they do for renals (ie, special stains, immunos, EM, etc.), the usual turnaround time, and total charge (both professional and technical). Thank you in advance for any information. You may reply to me personally or post on the Histonet. Martha Ward Wake Forest University Baptist Medical Center -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/5fe10994/attachment.htm From Sue.Kapoor <@t> uhsi.org Wed Dec 10 13:10:25 2003 From: Sue.Kapoor <@t> uhsi.org (Kapoor, Sue) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Saving Cytology Wet Specimens, CAP Regulation Message-ID: <61E9F2400F53D5119CFC00508B44E33B019F5515@khmcexch.uhsi.org> Hi all, can anyone tell me what the required time is to keep wet cytology specimens...such as urines, thoracentesis fluid, etc. I can't find the requirement for CAP regulations Thanks in advance, Sue Kapoor, HT (ASCP) Histology Supervisor Kenosha Medical Center Kenosha, WI From tflore <@t> lsuhsc.edu Wed Dec 10 13:12:16 2003 From: tflore <@t> lsuhsc.edu (Flores, Teresa) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] STAT renal, liver and pancreas charges/cpt codes Message-ID: Martha, I forgot to mention that the STAT renal or liver or pancreas needle biopsies received before 11am are given a preliminary report via HRLM studies on the same day! Teresa Flores LSUHSC Pathology, EM Lab New Orleans, LA Martha, sorry, I have been on vacation and just did return. Please go to our WEB site at http://www.lsuhsc.edu/no/schools/ms/departments/pathology/pathist/dx_ home.html I am checking to see if our charges are in our web site and if not, we are placing them in there, but here they are for they time being. For a biopsy to be processed STAT the requisition form must be labeled STAT. Remember that the cut off time for STATS is 11:00am. Specimens that arrive before 2pm will have 24 hour turn around time. Specimens that arrive after 2pm will have a 48 hour turn around time. Immunofluorescent (IF); High Resolution Light Microscopy (HRLM); Transmission Electron Microscopy (TEM) Level IV and Level V HRLM only CPT Code 88349 (Routine) $400.00 CPT Code 88349 (STAT) $500.00 CPT Code 88349 (Unsatisfactory) $160.00 CPT Code 88346 (Routine) Each antibody is $50.00 Performed are: IgG, IgM, IgA, C3, C1q, (C4d for transplants on request), Fibrinogen, Kappa and Lambda. CPT Code 88346 (Unsatisfactory) $50.00 CPT Code 88348 (Routine) $800.00 Price includes HRLM, TEM and immunofluorescent studies. CPT Code 88348 (STAT )$1000.00 -----Original Message----- From: Martha Ward [mailto:] Sent: Monday, December 01, 2003 10:38 AM ToSubject: [Histonet] Renal biopsy charges/procedures We are currently looking at our charges and procedures for renal biopsies and my manager wanted me to see if anyone on the Histonet would be willing to share what procedures they do for renals (ie, special stains, immunos, EM, etc.), the usual turnaround time, and total charge (both professional and technical). Thank you in advance for any information. You may reply to me personally or post on the Histonet. Martha Ward Wake Forest University Baptist Medical Center -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/96923501/attachment.htm From pruegg <@t> colobio.com Wed Dec 10 13:55:20 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] polymerization problems In-Reply-To: <00d401c3bf2a$62ab7000$38595b82@vet.upenn.edu> Message-ID: Tom, It sounds like you don?t have your components insinc. You must have enough activator (usually benzoyl peroxide) to react with the initiator (usually nn-dimethyl aniline and polyethylene glycol). Polymerization requires that the proper chemistry, volume and temperature combination be used. Perhaps it is too cold for your chemical polymerization reaction to begin with the volume of mix you are using. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tom Schaer Sent: Wednesday, December 10, 2003 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] polymerization problems Dear Histonetters: I am going to try again, realizing that not many have vast experience with embedding large specimen with technovit 9100 yet. Maybe someone may be able to share some pointers for a non-profit lab? Essentially, we fail to successfully polymerize med fem ovine condyles in approx 100ml jars. Tried at -15C with no change in solution consistency for a week; then brought it up to -2C still no change over three days. Jars were "vacuumed" and set in refrigerator / freezer. I am at the of my rope of troubleshooting. Thanks for any pointers. Tom ------------------------------------------ Thomas P. Schaer, VMD Assist. Prof. BIOMED, Drexel University Comparative Orthopaedic Research & Tissue Engineering Department of Clinical Studies University of Pennsylvania New Bolton Center 382 West Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office) tel. 610-444-5800 (x6131 lab) fax. 610-925-8100 tpschaer@mail.vet.upenn.edu -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/e12ccdc7/attachment.htm From Sue.STURROCK <@t> austin.org.au Wed Dec 10 15:45:03 2003 From: Sue.STURROCK <@t> austin.org.au (STURROCK, Sue) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] BioCare Decloaker Message-ID: Hi Guys, We have recently purchased the BioCare Decloaker and have been impressed with our improved antigen retrieval results. Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6. However, it has given us some problems with spiking of high temperatures and subsequent destruction of tissue sections. Also nuclear detail seems to be reduced when using this instrument. Has anyone else out there had problems like this and can we expect anything else? Sue Sturrock Austin Health Melbourne, Australia ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- This email contains confidential information intended only for the person named above and may be subject to legal privilege. If you are not the intended recipient, any use, disclosure, copying or distribution of this transmission is prohibited. If you have received this message in error, please notify us immediately by return email and delete the original email and any attachments. Austin Health provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/92493205/attachment.htm From SJones <@t> cvm.tamu.edu Wed Dec 10 15:44:38 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Rotring ink for marking arteries and veins Message-ID: Hello Netters, I had a researcher come in and ask about Rotring ink. It was used in a procedure to inject and mark the arteries and veins with different colors. I know Polysciences has the Batson's Anatomical Corrosion Kit. I spoke to Pam Marcum at Polysciences and she said the Batson's is not Rotring ink. She thought Rotring ink may be a tattoo ink. Does anyone know what Rotring ink is, where it can be purchased, and how it would be diluted to inject into the vessels? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From Sue.STURROCK <@t> austin.org.au Wed Dec 10 15:55:16 2003 From: Sue.STURROCK <@t> austin.org.au (STURROCK, Sue) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Somatostatin Receptor 2 Message-ID: Hi Guys, I am looking for an antibody to Somatostatin Receptor 2 for immunohistochemistry on paraffin sections. Is anyone using a good one out there? If so can you supply details for purchase and use? Thanks. Sue Sturrock Austin Health Melbourne, Australia ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- This email contains confidential information intended only for the person named above and may be subject to legal privilege. If you are not the intended recipient, any use, disclosure, copying or distribution of this transmission is prohibited. If you have received this message in error, please notify us immediately by return email and delete the original email and any attachments. Austin Health provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/57fe21ee/attachment.htm From pruegg <@t> colobio.com Wed Dec 10 16:28:02 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Research Genetics Message-ID: Remember this company? I know they are not around anymore but is someone else selling the great stuff they used to sell, like the stable DAB? Patsy From nick.kirk3 <@t> btopenworld.com Wed Dec 10 16:27:47 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Rotring ink for marking arteries and veins In-Reply-To: Message-ID: Hi Sarah Rotring are a company that make all sorts of pens including calligraphy pens, fountain pens marker pens etc. They also manufacture all sorts of inks to go with those pens. Your best bet would probably be a local stationer or arts supply shop. I have used their India ink in the past for marking excision margins. It worked quite well. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Sarah Jones Sent: 10 December 2003 21:45 To: histonet@pathology.swmed.edu Subject: [Histonet] Rotring ink for marking arteries and veins Hello Netters, I had a researcher come in and ask about Rotring ink. It was used in a procedure to inject and mark the arteries and veins with different colors. I know Polysciences has the Batson's Anatomical Corrosion Kit. I spoke to Pam Marcum at Polysciences and she said the Batson's is not Rotring ink. She thought Rotring ink may be a tattoo ink. Does anyone know what Rotring ink is, where it can be purchased, and how it would be diluted to inject into the vessels? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Wed Dec 10 16:56:32 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Research Genetics Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CE0E@usca0082k03.rallansci.apogent.com> Research genetics was taken over by Invitrogen http://www.invitrogen.com Tim Morken -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, December 10, 2003 2:28 PM To: Histonet@Pathology. Swmed. Edu Subject: [Histonet] Research Genetics Remember this company? I know they are not around anymore but is someone else selling the great stuff they used to sell, like the stable DAB? Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Wed Dec 10 17:01:31 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Immuno procedure for PrP with Hydrolytic Autoclavi ng Message-ID: I have done a fair amount of this what would you like to know? c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: David Clark [mailto:clarkda@ohsu.edu] Sent: Wednesday, December 10, 2003 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno procedure for PrP with Hydrolytic Autoclaving Hello, I'm about to embark on a new adventure. I'm wanting to stain some CJD material using a PrP antibody. The procedure requires hydrolytic autoclaving. Has anybody had any experience with this procedure? Any information would be greatly appreciated. Thank-you. David Clark Neuropathology OHSU Portland, Or _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Wed Dec 10 17:29:02 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] BioCare Decloaker Message-ID: Sue, I have used this routinely for a little over a year. I do agree some of the nuclear detail is less than wonderful but since we also have an H&E usually cut serially it is not so problematic. I think the temperature range is not a tight as I would like but I have found it to be very helpful. I usually use citrate @pH 6 122C 20 minutes. Have used various buffers and even dilute HCl and not had a problem with tissue destruction or sections falling off slides. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: STURROCK, Sue [mailto:Sue.STURROCK@austin.org.au] Sent: Wednesday, December 10, 2003 2:45 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] BioCare Decloaker Hi Guys, We have recently purchased the BioCare Decloaker and have been impressed with our improved antigen retrieval results. Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6. However, it has given us some problems with spiking of high temperatures and subsequent destruction of tissue sections. Also nuclear detail seems to be reduced when using this instrument. Has anyone else out there had problems like this and can we expect anything else? Sue Sturrock Austin Health Melbourne, Australia ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- This email contains confidential information intended only for the person named above and may be subject to legal privilege. If you are not the intended recipient, any use, disclosure, copying or distribution of this transmission is prohibited. If you have received this message in error, please notify us immediately by return email and delete the original email and any attachments. Austin Health provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/d3ade9de/attachment.htm From cytch7 <@t> cox-internet.com Wed Dec 10 20:00:10 2003 From: cytch7 <@t> cox-internet.com (cytch7@cox-internet.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Saving Cytology Wet Specimens, CAP Regulation Message-ID: <20031211020010.FTDB13168.fe1@localhost> There is no "required" time to save the specimens. We save ours in the refrigerator for a week and then toss them out. However, before throwing them away we verify that the case has been signed out. We will not throw it out until it's been signed out. > Subject: [Histonet] Saving Cytology Wet Specimens, CAP Regulation > > Hi all, can anyone tell me what the required time is to keep wet cytology > specimens...such as urines, thoracentesis fluid, etc. > Valerie N. Biendara SCT(ASCP)IAC Cytology Supervisor NW Arkansas Pathology Assoc. From caroline.stott <@t> anatomy.otago.ac.nz Wed Dec 10 20:10:52 2003 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] DAB kits Message-ID: <5.2.1.1.0.20031211150740.00a8add0@anatomy.otago.ac.nz> We have a batch sitting in our -25 freezer ready to be defrosted for use. We aliquot the components in about 1.5ml amounts and freeze them. They seem to work fine. And, you dont have to do it very often, therefore cutting down on exposure. Caroline Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 From m_masri80 <@t> yahoo.com Wed Dec 10 20:04:51 2003 From: m_masri80 <@t> yahoo.com (mashita masri) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] unsubscribe Message-ID: <20031211020451.79941.qmail@web41203.mail.yahoo.com> --------------------------------- Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031210/1930a74e/attachment.htm From alust1 <@t> hotmail.com Wed Dec 10 21:16:38 2003 From: alust1 <@t> hotmail.com (Andrew Lust) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] working with Vectashield with propidium Iodide Message-ID: Dear histonetters Just wanted to know if there is anything I need to do when handling Vectashield with Propidium Iodide? Sometimes the Vectashield propidium iodide works and sometimes it doesn't. Can anyone tell me what I need to do step by step so that i can get consistent results with vectashield PI. do you allow the section to dry before placing the vectashield (PI) then placing the cover slip. Or do you wash then wipe around the section and then place Vectashield with PI on the tissue then coverslip? How sensitive is Vectashield PI to light. andrew _________________________________________________________________ Don’t worry if your Inbox will max out while you are enjoying the holidays. Get MSN Extra Storage! http://join.msn.com/?PAGE=features/es From jkiernan <@t> uwo.ca Wed Dec 10 23:20:20 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] working with Vectashield with propidium Iodide References: Message-ID: <3FD7FE94.5CEC42A6@uwo.ca> Why don't you ask Vectashield how you should use their products? If they won't help you, then you should buy your immunohistochemical products from another company. There are many. ------------------------------ Andrew Lust wrote: > Can anyone tell me what I need to do > step by step so that i can get consistent results > with vectashield PI. From MinHan.Tan <@t> vai.org Wed Dec 10 23:47:59 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Frozen sections and loss of nuclei? Message-ID: <74D0F0AB07F2E647A02D839ED79520F94A9DE8@VAIEXCH02.vai.org> Hi there Histonetters, I would like to seek your advice about a problem I am having with frozen sections. I am working on some frozen tissue dating back to about 1998, and performing IHC on parathyroid tissue with p27 antibody. I use the DAB-ABC method. I have noted that certain samples (and particular samples only) exhibit loss of nuclei in the central region (an amorphous blob of tissue extending to near the margins, about 70-80% of the specimen) (no staining with hematoxylin either). Any nuclei that stain with my IHC in that area are very faint, and there is no hematoxylin counterstained nuclei in the entire region. Nuclear staining seems to be fine near the margins - of course it can be argued that these are artifacts. Where there are folds of tissue, both nuclear and background staining is prominent. Other samples of the same type of tissue seem to stain OK throughout the tissue. No problems with paraffin-embedded tissue at all, the nucleus stains fine in every sample I have tried. It is unlikely to be due to underfixation, since I immerse my 5 micron unfixed slide in neutral buffered formalin for 30 min prior to IHC. I see it regardless of whether I perform HIER on my frozen sections or not. (yes, I do perform HIER on my frozen sections) I wonder if the following explanations are plausible: (a) tissue has degraded - but it would not make sense for degradation to take place from inside out. (b) sections may be too thin for some reason? (mine are 5 micron) - but this might explain why only the borders and folds stain. Thank you! Min-Han Tan Van Andel Research Institute This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/7170d0bd/attachment.htm From mghoddusi <@t> cmri.usyd.edu.au Thu Dec 11 00:05:12 2003 From: mghoddusi <@t> cmri.usyd.edu.au (Majid Ghoddusi) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] LacZ staining on blood smear Message-ID: Hi All, If you were to do LacZ staining on blood smears what would be your fixative of choice? any tips and tricks about the protocol would be much appreciated as I am clueless on this one! Regards, Majid ........................................................... Majid Ghoddusi DVM, PhD Senior Microscopist Muscle Development Unit Children's Medical Research Institute Locked Bag 23, Wentworthville NSW 2145 Tel: (02) 9687-2800 Fax:(02) 9687-2120 www.cmri.com.au .............................................................. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/850ec911/attachment.htm From ctsblack <@t> capeheart.uct.ac.za Thu Dec 11 00:57:44 2003 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Unsubscibe Message-ID: -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From c.m.vanderloos <@t> amc.uva.nl Thu Dec 11 01:52:25 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] RE: IHC on cells on coverslips Message-ID: Dear Patsy, Please keep in mind that ethanol fixation as suggested by Tony Henwood, is very good for the cellular morphology, but not really beneficial to many epitopes/antigens! The acetone-fixation only works well for antigens at the cell surface or structures that are at least tightly bound to anything. If you are dealing with an antigen that may leak out (cytokines, hormones, grow factors, or any other small peptide) you need a 4% PFA fixation. Because cells grown on coverslips do have an intact outer membrane (and are not leaking!) the antigen of interest gets nicely fixed inside the cell. You need to add 0.1% saponin to all your reagents and buffers (from endogenous PO block up to chromogen step) to open up the cell membrane getting your reagents in and out. Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, 10 December 2003 9:12 AM To: Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: [IHCRG] IHC on cells on coverslips Please advise how to manage IHC staining for cells grown on coverslips. Patsy From Nancy.Walker <@t> sanofi-synthelabo.com Thu Dec 11 05:56:02 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] blue d'alcian or whole PAS after ISH Message-ID: Help, I need a quick answer don't have time to try to figure things out by myself and most of all only have a few slides so I don't want to lose the results. Will Alcian Blue staining alone at pH 4 or Alcian Blue +PAS destroy my photographic emulsion coating? I used Alcian Bl + PAS technique long ago and it was wonderful for identifying the different compartments of the GI tract and identifying the cell types and which were secreting more. Now I have an orphan receptor (all we know is it is a putative chloride channel activated by chloride) that the mRNA is expressed in the secreting cells of the GI tract but in a differential way, I want to see if the mRNA level is a function of the level of secretion. or cell types etc. So can I do a bl Alcian / PAS or one or the other without destroying my emulsion and theryby lose my signal?????????? Has anyone tried to do either of these techniques after microscopic photographic emulsion???? or if not can you suggest another technique??? faithfully yours, Nancy Walker Molecular Biology Scientist Sanofi-Synthelbo Research B.P. 37 Lab?ge Innopole 31676 LABEGE CEDEX FRANCE nancy.walker@sanofi-synthelabo.com tel : (33)561004179? fax :(33)561004001 From StarkusL <@t> ummhc.org Thu Dec 11 06:46:27 2003 From: StarkusL <@t> ummhc.org (Starkus, Laurie) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Frozen sections and loss of nuclei? Message-ID: Skipped content of type multipart/alternative From tony.j.savage <@t> gsk.com Thu Dec 11 07:53:52 2003 From: tony.j.savage <@t> gsk.com (tony.j.savage@gsk.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #167 - 24 msgs Message-ID: Nancy, In response to message 23 (Vol1 #167 - 24), Why don't you stain with alcian blue / PAS before you apply your emulsion. Both alcian blue and PAS should survive subsequent development. I have used this technique in the past for some of my autoradiographs. You would obviously have to assess whether or not you are compromising your in situ signal. However I would have thought you would be on safe ground if you just stained with alcian blue for a couple of minutes. Regards, Tony Histopathology Group Asthma Biology Department. RIRP CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764117 fax. +44 (0)1438 764782 email. Tony.J.Savage@gsk.com mobile +44 07753609835 http://ukdiscovery.gsk.com/histopathology/default.htm From FreidaC <@t> aol.com Thu Dec 11 07:57:45 2003 From: FreidaC <@t> aol.com (FreidaC@aol.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] False positive AFB Message-ID: >From personal experience I do know that acid fast organisms are present in some tap water. We cultured them from water after some bad experiences with false positives. Wang reported that somewhere over 30% of water fountains showed the presence of acid fast organisms (non-pathogens, but who can tell the difference on a stained slide). This was done using the fluorescence technique. As a consequence, we filtered our water bath water through a Millipore filter after it was already passed through a charcoal filter and a deionizing column. We also used no tap water prior to the carbol-fuchsin and we ran a negative control (uterus - extra cuts from blocks as the routine H&Es were cut) from the same days workload with each batch of AFB slides. So take care with your AFB stains. Freida Carson From CCLYATT <@t> mail.mcg.edu Thu Dec 11 08:20:52 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] False positive AFB Message-ID: Freida, Would it not be wise to identify AFB contamination in one's water source? If contamination is present, I think it could be assumed that AFB contamination could also enter the specimen by fixation and processing in house made 10% formalin, alcohol dilutions, etc. I routinely culture our deionized water once a month. Not being a microbiologist, I'm not sure if I'm eliminating the possibility of AFB contamination by only routine culture. Do I need to request an AFB culture? Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu >>> 12/11/03 08:57AM >>> >From personal experience I do know that acid fast organisms are present in some tap water. We cultured them from water after some bad experiences with false positives. Wang reported that somewhere over 30% of water fountains showed the presence of acid fast organisms (non-pathogens, but who can tell the difference on a stained slide). This was done using the fluorescence technique. As a consequence, we filtered our water bath water through a Millipore filter after it was already passed through a charcoal filter and a deionizing column. We also used no tap water prior to the carbol-fuchsin and we ran a negative control (uterus - extra cuts from blocks as the routine H&Es were cut) from the same days workload with each batch of AFB slides. So take care with your AFB stains. Freida Carson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bacceil <@t> labcorp.com Tue Dec 9 18:44:59 2003 From: Bacceil <@t> labcorp.com (Laurel Baccei) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Histotechnologist Job Opportunity Message-ID: LabCorp, one of the nation's largest and most successful clinical laboratories is searching for a histotech to work in our San Diego laboratory. We are looking for someone with histotech experience- we are willing to train. We offer a full benefits plan including 18 paid days off per year, tuition reimbursement, a pension plan, 401k with employer match, medical, dental, vision, you name it... we have it. Please apply online at www.labcorp.com/careers or fax your resume to 858.546.3937 Thank you for your time, Laurel Baccei, HR Consultant 858.455.1221 Laurel Baccei, HR Consultant www.LabCorp.com 858.455.1221x3104 ----------------------------------------- IMPORTANT WARNING: This e-mail and any files transmitted with it may contain CONFIDENTIAL information, including PRIVATE AND CONFIDENTIAL HEALTH INFORMATION which is intended for the use of the person to whom it is addressed. If the reader of this e-mail/attachment is not the intended recipient, employee, or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, reproduction, reading, or copying of this information is STRICTLY PROHIBITED. If you have received this e-mail in error, please delete the related e-mail and all attachments and notify the sender immediately (reply e-mail) and the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From japoteete <@t> saintfrancis.com Thu Dec 11 09:22:39 2003 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] False positive AFB Message-ID: As an old Medical Technologist turned IHC Technologist, yes, it is necessary to order an acid fast culture. Those bugs won't grow on the routine culture media. They also require more time to grow, so don't expect the Micro folks to give you a report in just a few days. Jacquie Poteete MT(ASCP), Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com > -----Original Message----- > From: Claye Clyatt [SMTP:CCLYATT@mail.mcg.edu] > Sent: Thursday, December 11, 2003 8:21 AM > To: FreidaC@aol.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] False positive AFB > > Freida, > > Would it not be wise to identify AFB contamination in one's water > source? If contamination is present, I think it could be assumed that > AFB contamination could also enter the specimen by fixation and > processing in house made 10% formalin, alcohol dilutions, etc. I > routinely culture our deionized water once a month. Not being a > microbiologist, I'm not sure if I'm eliminating the possibility of AFB > contamination by only routine culture. Do I need to request an AFB > culture? > > Claye > > Claye Clyatt > Chief Histotechnologist > Department of Pathology > Room #BF119 > Medical College of Georgia > Augusta, Ga 30912 > office (706) 721-3630 > pager (706) 721-7243-1132 > e-mail: cclyatt@mail.mcg.edu > > > >>> 12/11/03 08:57AM >>> > > From personal experience I do know that acid fast organisms are present > in > some tap water. We cultured them from water after some bad experiences > with > false positives. Wang reported that somewhere over 30% of water > fountains showed > the presence of acid fast organisms (non-pathogens, but who can tell > the > difference on a stained slide). This was done using the fluorescence > technique. > As a consequence, we filtered our water bath water through a Millipore > filter > after it was already passed through a charcoal filter and a deionizing > column. > We also used no tap water prior to the carbol-fuchsin and we ran a > negative > control (uterus - extra cuts from blocks as the routine H&Es were cut) > from > the same days workload with each batch of AFB slides. So take care > with your > AFB stains. > > Freida Carson > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From Nancy.Walker <@t> sanofi-synthelabo.com Thu Dec 11 09:41:09 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] more PAS on ISH Message-ID: I started my alcian PAS protocole, the alcian blue (1g /100ml in 3% glacial acetic acid) doesn't penetrate the emulsion and color the tissue but eats it away the emulsion (the silver grain ISH signals with it). Are there any alternative alcian blue protocols???? thanks, Nancy From donna <@t> phxbio.com Thu Dec 11 10:00:58 2003 From: donna <@t> phxbio.com (Donna Brown) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Research Genetics In-Reply-To: References: Message-ID: <6.0.0.22.0.20031211095624.02816f50@phxbio.com> Patsy- Some of the old Research Genetics crew have begun a new company called Phoenix BioTechnologies (yes, we're still in Huntsville...). We manufacture all the IHC/ISH solutions that were previously available from RG and more. Please contact me and I'll be glad to help you. Best regards- Donna >Remember this company? I know they are not around anymore but is someone >else selling the great stuff they used to sell, like the stable DAB? >Patsy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Dec 11 10:02:28 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] blue d'alcian or whole PAS after ISH References: Message-ID: <3FD89514.D068B9F5@uwo.ca> An alcian blue solution should not damage your developed and fixed autoradiographic emulsion. The periodic acid step of PAS might dissolve the silver grains. When I did autoradiography (labelled proteins or DNA) I stained the sections before coating them with the emulsion. In your tissues that's probably not appropriate because it might extract the mRNA that you're interested in. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ___________________________ Nancy.Walker@sanofi-synthelabo.com wrote: > > Help, I need a quick answer don't have time to try to figure things out by > myself and most of all only have a few slides so I don't want to lose the > results. > > Will Alcian Blue staining alone at pH 4 or Alcian Blue +PAS destroy my > photographic emulsion coating? I used Alcian Bl + PAS technique long ago > and it was wonderful for identifying the different compartments of the GI > tract and identifying the cell types and which were secreting more. Now I > have an orphan receptor (all we know is it is a putative chloride channel > activated by chloride) that the mRNA is expressed in the secreting cells of > the GI tract but in a differential way, I want to see if the mRNA level is > a function of the level of secretion. or cell types etc. So can I do a bl > Alcian / PAS or one or the other without destroying my emulsion and theryby > lose my signal?????????? > > Has anyone tried to do either of these techniques after microscopic > photographic emulsion???? or if not can you suggest another technique??? > > faithfully yours, > Nancy Walker > Molecular Biology Scientist > > Sanofi-Synthelbo Research > B.P. 37 Lab?ge Innopole > 31676 LABEGE CEDEX FRANCE > > nancy.walker@sanofi-synthelabo.com > tel : (33)561004179 fax :(33)561004001 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Dec 11 10:10:34 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] more PAS on ISH References: Message-ID: <3FD896FA.2DCA7371@uwo.ca> Nancy.Walker@sanofi-synthelabo.com wrote: > I started my alcian PAS protocole, the alcian blue (1g /100ml in 3% glacial > acetic acid) doesn't penetrate the emulsion and color the tissue but eats > it away the emulsion (the silver grain ISH signals with it). Are there any > alternative alcian blue protocols???? That's surprising. Is the emulsion fixed and hardened? You need to use a combined fixer-hardener containing alum as well as Na2S2O3. We use the same general purpose Kodak fixer that's used for films and papers. Na2S2O3 alone will fix (remove unexposed silver halides) but will not harden the emulsion. Hardening is similar to fixing in the histological sense. The Al3+ ions from the alum cross-link gelatin molecules of the emulsion. The alcian blue solution you describe, in 3% acetic acid, should have a pH of 2.5. In your earlier email you said pH 4. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From donna <@t> phxbio.com Thu Dec 11 10:11:29 2003 From: donna <@t> phxbio.com (Donna Brown) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Research Genetics In-Reply-To: References: Message-ID: <6.0.0.22.0.20031211100437.027ffb90@phxbio.com> After Invitrogen acquired and closed Research Genetics in Huntsville, several of the RG crew began a new company called Phoenix BioTechnologies (yes, we're still in Huntsville...) to manufacture all the IHC/ISH solutions (I understand that Invitrogen has discontinued many of these solutions). We also provide DNA synthesis of multibiotin ISH probes. Please contact me and I'll be glad to help you. Best regards- Donna Donna Brown dbrown@phxbio.com http://www.phxbio.com Phoenix BioTechnologies, Inc. 1000 Meridian Street Huntsville, AL 35801 Toll-free 866-319-0900 FAX 256-319-0902 >Remember this company? I know they are not around anymore but is someone >else selling the great stuff they used to sell, like the stable DAB? >Patsy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/d9c691b2/attachment.htm From FreidaC <@t> aol.com Thu Dec 11 10:12:23 2003 From: FreidaC <@t> aol.com (FreidaC@aol.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] False positive AFB Message-ID: <14a.27fef8db.2d09f167@aol.com> You are correct that house made formalin and other reagents can contain AFB if the water is contaminated - that is one reason that we always used a negative control from the current day's workload. This is a check on fixation and processing as well as on the staining procedure. I would suggest that you repeatedly centrifuge 50 ml aliquots of tap water, decanting and centrifuging another 50 ml until you can see some sediment in the centrifuge tube. Take this to microbiology and have them culture for AFB. In researching this problem, we noted that AFB has also been reported in paraffin (the old Tissue Mat wafers) and growing in a 40 gal. formalin tank. It is a problem that is not taken into consideration often enough. Freida From mprice26 <@t> juno.com Thu Dec 11 10:14:40 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Body Lift for Morgue/Autopsy Room Message-ID: <20031211.081517.3579.1178157@webmail10.nyc.untd.com> Histonetters, Can anyone tell me where a good source is to buy a body lift. I have Mopecs' Catalog. Just wondering if there were any other companies that sell Morgue supplies. Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From livieira <@t> ualg.pt Thu Dec 11 10:36:01 2003 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Toluidine blue solution: Thanks for your help! Message-ID: <008701c3c004$dccb50a0$2914100a@labhistologia> I want to say thankyou, to John Kiernan and Geoff McAuliffe, for your helpful information about the solution of Toluidine Blue. Thanks again, Lina Vieira Universidade do Algarve Faro, Portugal -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/58c6c35d/attachment.htm From douglas_moore <@t> Brown.edu Thu Dec 11 11:57:07 2003 From: douglas_moore <@t> Brown.edu (Douglas_Moore) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Skeletal Histology Reference Books Message-ID: Can anyone recommend a good histology techniques manual that focuses on skeletal tissue (bone, cartilage, ligament, etc.) and techniques? I'm looking for something that describes the rationale for selecting different stains, gives examples, and gives recipes for how to make them. I'll certainly summarize and post all of the replies, Doug -- __________________________________________________ Douglas C. Moore, M.S. Assoc. Dir. Bioengineering Laboratory Sr. Research Associate, Department of Orthopaedics Brown Medical School/Rhode Island Hospital Bioengineering Laboratory CORO West, Suite 404 1 Hoppin Street Providence, RI 02903 Vox: 401.444.8904 Fax: 401.444.4418 email: douglas_moore@brown.edu From histobabs <@t> hotmail.com Thu Dec 11 12:09:37 2003 From: histobabs <@t> hotmail.com (Barbara Terrett) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] vasopressin Message-ID: Hi All, I was wondering what vasopressin antibody people are using for formalin fixed paraffin embedded tissue. Thanks Barb _________________________________________________________________ Protect your PC - get McAfee.com VirusScan Online http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From clarkda <@t> ohsu.edu Thu Dec 11 12:32:18 2003 From: clarkda <@t> ohsu.edu (David Clark) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Donation of Chemicals/Dyes Message-ID: Hello, A retired professor from the Dental School here in Portland, Or. has a huge array of chemicals that he would like to donate to a worthwhile cause. He has had these for sometime so I am not sure what kind of dates he has on these. Many, I am sure are outdated but are still sealed. If anyone is interested or know of anyone who may be interested please let me know. David Clark Neuropathology OHSU Portland, Or From peoshel <@t> wisc.edu Thu Dec 11 12:39:25 2003 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] archival fat storage Message-ID: Micro Mavens, I have a person who has stained macrophages with Oil Red O for fat, and now needs to make slides of them to be archived. The cells are on plastic coverslips, and will be fixed with formalin. Cultured cells, so no sectioning, etc. I have the usual references for aqueous mounting media, but I'm wondering about the archival bit. I have no problem, unfortunately, with archival storage of my personal fat, but making aqueous fat mounts on slides that are archival is a different matter. What is the best way to do this? (For room temperature storage.) Thanks. Phil -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From haldana <@t> unimoron.edu.ar Thu Dec 11 12:56:57 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Skeletal Histology Reference Books References: Message-ID: <006001c3c018$8f1b8780$7504a8c0@um.edu> Hi I have the wonderfull book the "Theory and Practice of Histological Techniques" (Bancroft and Stevens- Churchill livingstone editorial) it has a lot of techniques and simple explanations for connective tissue (Chap 8 and 16 (Chapter related to bone)). Also another wonderfull book is "The theory and Practice of Histotechnology of Sheenan and Hrapchak (Mosby editorial)It also has a great chapter dedicated to Connective and muscle fiber stains (Ch 10) and one perfect chapter dedicated to bone, also inclueded decalcification techniques and histomorphometry (Ch 6). Regrettably I dont know the Kiernan?s book By Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: "Douglas_Moore" To: Sent: Thursday, December 11, 2003 2:57 PM Subject: [Histonet] Skeletal Histology Reference Books > Can anyone recommend a good histology techniques manual that focuses > on skeletal tissue (bone, cartilage, ligament, etc.) and techniques? > I'm looking for something that describes the rationale for selecting > different stains, gives examples, and gives recipes for how to make > them. > > I'll certainly summarize and post all of the replies, > > Doug > > -- > __________________________________________________ > Douglas C. Moore, M.S. > Assoc. Dir. Bioengineering Laboratory > Sr. Research Associate, Department of Orthopaedics > Brown Medical School/Rhode Island Hospital > > Bioengineering Laboratory > CORO West, Suite 404 > 1 Hoppin Street > Providence, RI 02903 > Vox: 401.444.8904 > Fax: 401.444.4418 > email: douglas_moore@brown.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Barry.R.Rittman <@t> uth.tmc.edu Thu Dec 11 13:58:22 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] archival fat storage Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FB8E@UTHEVS3.mail.uthouston.edu> Phil Although oil red O often survives archiving it doesn't always. Have you considered seeing whether you can expose the tissue to osmium tetroxide vapor, could then process to permanent mountant and have better resolution? I see no reason that this would not work and might give you a more permanent preparation. Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Philip Oshel Sent: Thursday, December 11, 2003 12:39 PM To: Histonet@Pathology.swmed.edu Subject: [Histonet] archival fat storage Micro Mavens, I have a person who has stained macrophages with Oil Red O for fat, and now needs to make slides of them to be archived. The cells are on plastic coverslips, and will be fixed with formalin. Cultured cells, so no sectioning, etc. I have the usual references for aqueous mounting media, but I'm wondering about the archival bit. I have no problem, unfortunately, with archival storage of my personal fat, but making aqueous fat mounts on slides that are archival is a different matter. What is the best way to do this? (For room temperature storage.) Thanks. Phil -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Myri37 <@t> aol.com Thu Dec 11 14:17:16 2003 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] SEM collagen fibers Message-ID: <29.4d1632b1.2d0a2acc@aol.com> hello dear histonetters first many thanks for your last precious help I have few questions about identification of collagen fibers in resconstructed tissue Do you think that fixation of small tissue with glutarald?hyde for three days can avoid proper stainning of collagen fibers with trichrome stains, should i change the fixative to paraformald?hyde for instance or reduce time of fixation ? Do you know if it's possible to do SEM observations on sections which have been previously embedded in methylmetacrylate or paraffine ? the issue is as well to observe collagen fibers and their orientation i have no experience to doing that, does someone have a protocol ? thank you so much for anyhelp Myriam baali Natural implant 163, av de luminy marseille, france tel: 04 91 82 65 23 Fax: 04 91 82 65 21 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/c02aa4e0/attachment.htm From AnthonyH <@t> chw.edu.au Thu Dec 11 16:13:28 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] RE: IHC on cells on coverslips Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E0FE@simba.kids> Chris, "Not really beneficial to many antigens"? I could list at least 50 antibodies that work on ethanol fixed smears. Most of these are in routine use. eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA, Skeletal muscle myosin, Actin,etc etc. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: C.M. van der Loos [mailto:c.m.vanderloos@amc.uva.nl] Sent: Thursday, 11 December 2003 6:52 PM To: Histonet@pathology.swmed.edu Cc: pruegg@colobio.com; anthonyh@chw.edu.au Subject: RE: IHC on cells on coverslips Dear Patsy, Please keep in mind that ethanol fixation as suggested by Tony Henwood, is very good for the cellular morphology, but not really beneficial to many epitopes/antigens! The acetone-fixation only works well for antigens at the cell surface or structures that are at least tightly bound to anything. If you are dealing with an antigen that may leak out (cytokines, hormones, grow factors, or any other small peptide) you need a 4% PFA fixation. Because cells grown on coverslips do have an intact outer membrane (and are not leaking!) the antigen of interest gets nicely fixed inside the cell. You need to add 0.1% saponin to all your reagents and buffers (from endogenous PO block up to chromogen step) to open up the cell membrane getting your reagents in and out. Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands -----Original Message----- From: Patsy Ruegg [mailto:pruegg@colobio.com] Sent: Wednesday, 10 December 2003 9:12 AM To: Histonet@Pathology. Swmed. Edu Cc: Ihcrg@Yahoogroups. Com Subject: [IHCRG] IHC on cells on coverslips Please advise how to manage IHC staining for cells grown on coverslips. Patsy ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From joeamateur <@t> hotmail.com Thu Dec 11 17:33:11 2003 From: joeamateur <@t> hotmail.com (Jack England) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Summary of hand-processing/vacuum advice given to the Rookie Message-ID: Thanks so much to all for your advice; we used the infiltrator yesterday and are eager to see how everything pans out. In case someone else out there is facing a similar situation, here's the advice that came back (for those zen masters among you, if any of my information here is incorrect or needs clarification, by all means *please* correct me): --Consult a good textbook. Recommended in particular was Kiernan, J. (1999), Histological & Histochemical Methods, 3rd Ed, Arnold, ISBN: 0750649364. --Samples should be cleared for no more than 2 hours in room temp. xylene prior to infiltration, with the xylene changed out at least once, and all solution containers having volumes well in excess of the tissue being processed. --After coming out of xylene, go through at least one change of paraffin prior to infiltration (we're use three baths, then proceeding to the vacuum bath). Make sure that paraffin baths are of a fairly large volume relative to the tissue being infiltrated, so that the vast majority of xylene can be displaced with paraffin prior to heated vacuum. Also make sure that the paraffin used for infiltration is no greater than +2 degrees C of the melting point. --For the truly ambitious, vacuum can be applied to each of the multiple paraffin baths, provided that either the wax is changed between baths or there are multiple baths used. --Amount of vacuum to be applied is more art than science (as I'm learning are so many things about histology...*grin*). 40-200mm Hg seems to be acceptable, but trial and error seems to be the best way to find the sweet spot. Likewise, the amount of time under vacuum seems to be highly variable; we're using 1 hour under vacuum for our samples, but up to 4 hours was recommended as well. Again, trial and error is the way to go. --After infiltration, release vacuum SLOWLY so as not to a) gum up the needle valve in the infiltrator, or worse b) spray paraffin all over the lab. There was a lot more advice, and I can't tell you all how much I greatly appreciate you offering it. I was expecting a lot harsher response than I got ("take a class, buddy!" comes to mind), and I'm amazed by the support you've all provided. Hopefully down the line I'll be able to help out someone else the same way you've helped me. --Best Regards to all, Jack England _________________________________________________________________ Tired of slow downloads and busy signals? Get a high-speed Internet connection! Comparison-shop your local high-speed providers here. https://broadband.msn.com From boju <@t> ripnet.com Thu Dec 11 18:12:23 2003 From: boju <@t> ripnet.com (Judy Stead) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Unsubscribe Message-ID: <3FD907E7.000008.18347@Judy> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 285 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/61ca93f6/attachment.gif -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 494 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/61ca93f6/attachment-0001.gif -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 1431 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/61ca93f6/attachment.jpg From kwuny <@t> email.cs.nsw.gov.au Thu Dec 11 19:18:52 2003 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] L.A.B. Solution Message-ID: <000f01c3c04d$e7b4dab0$a6e04c98@lab0220.cs.nsw.gov.au> Hi Histonetters, Has anyone ever tried Polysciences' Liberate Antibody Binding (LAB) Solution? The product (www.polysciences.com Cat.No. 24310) is supposed to exposure the antigenic site of the FFPE tissue sections by incubating at room temperature for 5-20 minutes, eliminating the used of heating (HIER)!! This would give enormous changes the way we do immunostaining so far. Among few antibodies I tried so far, only LCA (CD45) and CD20 (L-26) were good enough and Progesterone receptor showed a weak staining. Regards Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au "the universe we observe has precisely the properties we should expect if there is, at bottom, no design, no purpose, no evil and no good, nothing but blind, pitiless indifference." "To err is human, to forgive is even more human" The information contained in this message is intended for the named addressee only, and is confidential to the sender and intended recipient. If you are not the named addressee please do not copy, distribute, take any action reliant on, or disclose anything in this E-mail message to any other person or organisation. If you have received this message in error please delete the email and notify me immediately. Views expressed in this message are those of the individual sender and are not necessarily the views of Central Sydney Area Health Service. From gagermeierjp <@t> upmc.edu Thu Dec 11 21:03:02 2003 From: gagermeierjp <@t> upmc.edu (Gagermeier, James) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Laser Microdissection of Lung Message-ID: <9122C182D4268F45BCB9E64A10B7331F024D114C@1upmc-msx7.isdip.upmc.edu> First time on the Histonet - These are questions that pertain to laser microdissection on lung tissue using a Leica AS LMD with the goal of performing microarray. If anyone can answer one, all of some of these questions I appreciate your input. 1) In staining tissues with nuclease free alcohols - Arcturus recommends that these alcohols be changed every 4 slides. I have noticed that using the alcohols for 10-12 slides does seem to lead to more difficult capture of the cells as they seem to adhere to the slides more tightly and do not drop into the caps. In an attempt to be economical, does anyone have recommendations for how many slides can be stained before changing alcohols/water is necessary? 2) I have ordered glass foiled slides to capture the cells, as opposed to glass slides, as I have been told that the foiled slides retain H2O less avidly - furthermore, it would seem that cutting through foil would naturally be easier than through the slides. 3) I have also read that if glass slides are used, that these too should be cleaned in DEPC-treated solution, autoclaved, and even RNAZapped. This seems a bit much - is this true? 4) In regards to the Leica system - is amplification of tissue almost universally necessary to obtain adequate amounts of RNA (lower limit .2 ug) to perform a Codelink platform microarray? Initially, I obtained a yield of about .3 ug from microdissected tissues. This yield was obtained from capturing a total area of 495,000 um2 of tissue from about 10 individual captured pieces of tissue (ranging in size from 9,000 - 180,000 um2). However, in subsequent sessions of microdissection, similar areas of tissue gave me a total of 40-120 nannograms of RNA - which will obviously need amplified. Should I expect to amplify most of the time? 5) In lung tissue, what is the minimum tissue area that needs to be captured ( if anyone wants to give me their thoughts) that is sufficient, then, to avoid amplification? 6) No number 6. Thanks, Jim Gagermeier -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/5cbe89b8/attachment.htm From Roasthare <@t> aol.com Thu Dec 11 22:26:33 2003 From: Roasthare <@t> aol.com (Roasthare@aol.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] need mohs histotech for Monterey, CA Message-ID: <57B3E9B0.670C7D11.028B0AE1@aol.com> A part-time Mohs histotech position is available in Monterey,CA. The position requires a minimum of 2 days weekly and may expand to four days weekly within a few months. Interested applicants should fax resumes to 831-372-0266. From RSRICHMOND <@t> aol.com Thu Dec 11 22:37:51 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Re: False positive AFB Message-ID: <1e6.1544fae1.2d0aa01f@aol.com> The acid-fast bacilli that come out of the water faucet are supposedly Mycobacterium gordoneae (gor-DOH-nee-ee, after microbiologist Mary Gordon) - a synonym is Myco. aquae (ACK-wee). They aren't pathogenic (well, maybe in situations of extremely compromised immune function). They stain with both light-microscopic and fluorescent techniques. The trick to recognizing them is that usually they're slightly out of the plane of the section, and in tissue sections they turn up at random rather than in places in tissues where a pathologist will expect to see them. Bob Richmond Samurai (SAM-you-rye) Pathologist (paTHOLLajist) Knoxville (NOCKSvil) TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031211/74b786e4/attachment.htm From lpwenk <@t> covad.net Fri Dec 12 04:17:01 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] HT salary Message-ID: <00c701c3c099$161c7980$8732fea9@hppav> Not to beat a dead horse . . . But now that we've told this person that their wage is unrealistic, I thought I'd let people know that the ASCP Board of Registry home page has the latest wage and vacancy surveys available on line. The survey was done in late 2002, but was just published in "Laboratory Medicine" in the fall of 2003. Please remember that your area/city on the country may be higher or lower (e.g., in the Detroit area, we are about $2/hour higher). No, it doesn't list universities separately. And I know from my student being hired, that universities do pay less, but often it is off-set by not having to pay to attend classes, or by getting great discounts on tuition fees. http://www.ascp.org/bor/center/center_research.asp (note: the "space" between center and research is an underline - center_research) In summary for HT - (Listed as the average beginning/middle/top salary per hour) Average of all HT jobs = $13.66/$16.61/$18.59 Hospital = 13.66/16.71/18.74 Reference/private lab = 13.34/15.50/18.00 <100 beds = 12.75/15.50/17.86 100-299 beds = 13.53/16.13/18.00 300-499 beds = 13.50/16.35/18.93 500+ beds = 14.05/17.85/19.68 Rural = 12.88/15.29/17/07 Suburb = 14.75/18.03/20.81 Small/medium city = 13.05/16.05/18.00 Large city = 15.00/18.00/19.91 Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lpwenk <@t> covad.net Fri Dec 12 04:21:46 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] ACE Message-ID: <00cf01c3c099$bfb14160$8732fea9@hppav> Need help with the ACE (acetylcholine esterase) procedure for Hirshsprung Disease. Two choices: 1. Can someone tell me a source of ethopropazine? We are no longer able to obtain it through Sigma/Aldrich. We did order it from another company, but now cannot get the stain to work. OR 2. Can someone share with me their procedure, which does not include this chemical. Thank you. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From ctsblack <@t> capeheart.uct.ac.za Fri Dec 12 04:34:10 2003 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] unsubscribe!! Message-ID: -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From louise_renton <@t> hotmail.com Fri Dec 12 04:58:47 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] polymerization problems Message-ID: Dear Tom, Well, this is my 2 cents (South African) worth: 1. I have, as yet, not been able to get the stuff to polymerise at cold temperatures. I have had very good sucess at RT, although with such large tissues, trial and error as well as judicious advice from fellow histonetters, it is recommended that the specimen is "layered" with embedding solution and allowed to polymerise over a few days. Otherwise unsightly and potentially bubbly and uncutable blocks result. 2. I have found that, because the 2 embedding solutions are so viscous that they must be well mixed before use. I generally stir mine for about 20 minutes on a magnetic stirrer before pouring into the mould. (do this in a fume hood) 3. Heraeus Kulzer have a very nice technical assistant in Germany who has been very helpful in the past. If all else fails get hold of her for further insights. She is Ulrike Leins at: Ulrike.Leins@t-online.de best Regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "Tom Schaer" To: Subject: [Histonet] polymerization problems Date: Wed, 10 Dec 2003 09:32:05 -0500 Dear Histonetters: I am going to try again, realizing that not many have vast experience with embedding large specimen with technovit 9100 yet. Maybe someone may be able to share some pointers for a non-profit lab? Essentially, we fail to successfully polymerize med fem ovine condyles in approx 100ml jars. Tried at -15C with no change in solution consistency for a week; then brought it up to -2C still no change over three days. Jars were "vacuumed" and set in refrigerator / freezer. I am at the of my rope of troubleshooting. Thanks for any pointers. Tom ------------------------------------------ Thomas P. Schaer, VMD Assist. Prof. BIOMED, Drexel University Comparative Orthopaedic Research & Tissue Engineering Department of Clinical Studies University of Pennsylvania New Bolton Center 382 West Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office) tel. 610-444-5800 (x6131 lab) fax. 610-925-8100 tpschaer@mail.vet.upenn.edu _________________________________________________________________ Accurate, relevant information a mouse-click away on MSN Search! http://search.msn.co.za/default.aspx From louise_renton <@t> hotmail.com Fri Dec 12 05:05:43 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Rotring ink for marking arteries and veins Message-ID: Rotring ink was extensively used for fine line drawing and by draughtsmen(women) before the advent of CAD/CAM systems. It is probably based on an indian ink formula*, but has some other sticky vehicle in it in it that would clog up the fine pens used if they were not cleaned properly. I used to use my sister's one for tracing drawings, and woe betide me if I gummed up the pens! *indian ink, originally a mixture of carbon black and shellac, is, if translated from the French, Chinese ink. They were probably the first to use it. Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "Sarah Jones" To: Subject: [Histonet] Rotring ink for marking arteries and veins Date: Wed, 10 Dec 2003 15:44:38 -0600 Hello Netters, I had a researcher come in and ask about Rotring ink. It was used in a procedure to inject and mark the arteries and veins with different colors. I know Polysciences has the Batson's Anatomical Corrosion Kit. I spoke to Pam Marcum at Polysciences and she said the Batson's is not Rotring ink. She thought Rotring ink may be a tattoo ink. Does anyone know what Rotring ink is, where it can be purchased, and how it would be diluted to inject into the vessels? Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Accurate, relevant information a mouse-click away on MSN Search! http://search.msn.co.za/default.aspx From louise_renton <@t> hotmail.com Fri Dec 12 05:24:45 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] BioCare Decloaker Message-ID: Realising that nothing in this world is perfect, am I the only one that has a small problem with Cynthia's statement: "I have used this routinely for a little over a year. I do agree some of the nuclear detail is less than wonderful but since we also have an H&E usually cut serially it is not so problematic. I think the temperature range is not a tight as I would like but I have found it to be very helpful. " Is it not incumbent on the technologists to get as morphologically accurate a section as possible, whether IHC or not, as an aide to diagnosis? What do the pathologists think? regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 ----- Hi Guys, We have recently purchased the BioCare Decloaker and have been impressed with our improved antigen retrieval results. Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6. However, it has given us some problems with spiking of high temperatures and subsequent destruction of tissue sections. Also nuclear detail seems to be reduced when using this instrument. Has anyone else out there had problems like this and can we expect anything else? Sue Sturrock Austin Health Melbourne, Australia ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- This email contains confidential information intended only for the person named above and may be subject to legal privilege. If you are not the intended recipient, any use, disclosure, copying or distribution of this transmission is prohibited. If you have received this message in error, please notify us immediately by return email and delete the original email and any attachments. Austin Health provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- _________________________________________________________________ Browse hundreds of hot categories on MSN Search! http://search.msn.co.za/default.aspx From Terry.Marshall <@t> rothgen.nhs.uk Fri Dec 12 06:04:16 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] BioCare Decloaker Message-ID: What do I think? Will you marry me:-)? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: louise renton [mailto:louise_renton@hotmail.com] Sent: 12 December 2003 11:25 To: cfavara@niaid.nih.gov; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BioCare Decloaker Realising that nothing in this world is perfect, am I the only one that has a small problem with Cynthia's statement: "I have used this routinely for a little over a year. I do agree some of the nuclear detail is less than wonderful but since we also have an H&E usually cut serially it is not so problematic. I think the temperature range is not a tight as I would like but I have found it to be very helpful. " Is it not incumbent on the technologists to get as morphologically accurate a section as possible, whether IHC or not, as an aide to diagnosis? What do the pathologists think? regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 ----- Hi Guys, We have recently purchased the BioCare Decloaker and have been impressed with our improved antigen retrieval results. Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6. However, it has given us some problems with spiking of high temperatures and subsequent destruction of tissue sections. Also nuclear detail seems to be reduced when using this instrument. Has anyone else out there had problems like this and can we expect anything else? Sue Sturrock Austin Health Melbourne, Australia ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- This email contains confidential information intended only for the person named above and may be subject to legal privilege. If you are not the intended recipient, any use, disclosure, copying or distribution of this transmission is prohibited. If you have received this message in error, please notify us immediately by return email and delete the original email and any attachments. Austin Health provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- _________________________________________________________________ Browse hundreds of hot categories on MSN Search! http://search.msn.co.za/default.aspx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alexander.nader <@t> wgkk.sozvers.at Fri Dec 12 06:36:00 2003 From: alexander.nader <@t> wgkk.sozvers.at (Nader, Alexander) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] RE: IHC on cells on coverslips Message-ID: <4FE843CBBAC1D211A8150008C70952DAAE7BDC@hk01nt05.hkh.wgkk.sozvers.at> > "Not really beneficial to many antigens"? I could list at least 50 > antibodies that work on ethanol fixed smears. Most of these > are in routine > use. > eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA, > Skeletal muscle myosin, Actin,etc etc. > S100-Antigen, GCFBP and some other ab's "leach out" with ethanol fixation, even fixation of trephines with Schaffer's solution (ethanol/formalin mixture) shows this unwanted and rather nasty effect. Alexander Nader MD Vienna, Austria From louise_renton <@t> hotmail.com Fri Dec 12 06:44:06 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] BioCare DecloakerOT Message-ID: as long as my husband has no objection : >). Remember, the price for bigamy is 2 mothers in law! Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" What do I think? Will you marry me:-)? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: louise renton [mailto:louise_renton@hotmail.com] Sent: 12 December 2003 11:25 To: cfavara@niaid.nih.gov; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BioCare Decloaker Realising that nothing in this world is perfect, am I the only one that has a small problem with Cynthia's statement: "I have used this routinely for a little over a year. I do agree some of the nuclear detail is less than wonderful but since we also have an H&E usually cut serially it is not so problematic. I think the temperature range is not a tight as I would like but I have found it to be very helpful. " Is it not incumbent on the technologists to get as morphologically accurate a section as possible, whether IHC or not, as an aide to diagnosis? What do the pathologists think? regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 ----- Hi Guys, We have recently purchased the BioCare Decloaker and have been impressed with our improved antigen retrieval results. Especially for antigens such as Thyroid Transcription Factor 1 and Cytokeratin cocktail 5 & 6. However, it has given us some problems with spiking of high temperatures and subsequent destruction of tissue sections. Also nuclear detail seems to be reduced when using this instrument. Has anyone else out there had problems like this and can we expect anything else? Sue Sturrock Austin Health Melbourne, Australia ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- This email contains confidential information intended only for the person named above and may be subject to legal privilege. If you are not the intended recipient, any use, disclosure, copying or distribution of this transmission is prohibited. If you have received this message in error, please notify us immediately by return email and delete the original email and any attachments. Austin Health provides no guarantee that this transmission is free of virus or that it has not been intercepted or altered. ---------------------------------------------------------------------------- ---------------------------------------------------------------------------- ------- _________________________________________________________________ Browse hundreds of hot categories on MSN Search! http://search.msn.co.za/default.aspx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Accurate, relevant information a mouse-click away on MSN Search! http://search.msn.co.za/default.aspx From histolog <@t> fcv.unl.edu.ar Fri Dec 12 08:13:02 2003 From: histolog <@t> fcv.unl.edu.ar (Lab Histologia) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Formaldehyde In-Reply-To: Message-ID: <002101c3c0ba$11e0c8a0$5a0cd2aa@unl.edu.ar> Hi All, I need to know if exists some method for to inactivate or neutralize formaldehyde. Thank you ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ From Barry.R.Rittman <@t> uth.tmc.edu Fri Dec 12 08:25:57 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] polymerization problems Message-ID: <566FB0B522443D43AF02D2ADBE35A6F06358DC@UTHEVS3.mail.uthouston.edu> Two comments First is that with mineralized tissues there will be a tendency for some regions with high mineral content to act as focal points for polymerization to start and consequently this often results in bubbles starting at that point. The second is that I have often wondered if thick solutions of methacrylate and other resins should be subjected to ultrasonic treatment before use. We used this method when mixing Epon and other resins for electron microscopy and it virtually eliminated bubble formation around tissues. Just a thought. Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Friday, December 12, 2003 4:59 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] polymerization problems Dear Tom, Well, this is my 2 cents (South African) worth: 1. I have, as yet, not been able to get the stuff to polymerise at cold temperatures. I have had very good sucess at RT, although with such large tissues, trial and error as well as judicious advice from fellow histonetters, it is recommended that the specimen is "layered" with embedding solution and allowed to polymerise over a few days. Otherwise unsightly and potentially bubbly and uncutable blocks result. 2. I have found that, because the 2 embedding solutions are so viscous that they must be well mixed before use. I generally stir mine for about 20 minutes on a magnetic stirrer before pouring into the mould. (do this in a fume hood) 3. Heraeus Kulzer have a very nice technical assistant in Germany who has been very helpful in the past. If all else fails get hold of her for further insights. She is Ulrike Leins at: Ulrike.Leins@t-online.de best Regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" ----Original Message Follows---- From: "Tom Schaer" To: Subject: [Histonet] polymerization problems Date: Wed, 10 Dec 2003 09:32:05 -0500 Dear Histonetters: I am going to try again, realizing that not many have vast experience with embedding large specimen with technovit 9100 yet. Maybe someone may be able to share some pointers for a non-profit lab? Essentially, we fail to successfully polymerize med fem ovine condyles in approx 100ml jars. Tried at -15C with no change in solution consistency for a week; then brought it up to -2C still no change over three days. Jars were "vacuumed" and set in refrigerator / freezer. I am at the of my rope of troubleshooting. Thanks for any pointers. Tom ------------------------------------------ Thomas P. Schaer, VMD Assist. Prof. BIOMED, Drexel University Comparative Orthopaedic Research & Tissue Engineering Department of Clinical Studies University of Pennsylvania New Bolton Center 382 West Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office) tel. 610-444-5800 (x6131 lab) fax. 610-925-8100 tpschaer@mail.vet.upenn.edu _________________________________________________________________ Accurate, relevant information a mouse-click away on MSN Search! http://search.msn.co.za/default.aspx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pam <@t> ategra.com Fri Dec 12 08:42:39 2003 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Histology openings Latest Update 12/12/03 Message-ID: Hi histonetters, Happy Holidays!!! Here is the latest update on opportunities with some of my best clients throughout the US who are seeking Histology Supervisors, Histo Technologists and Histo Technicians. These are positions as direct employees of our client. These are fulltime 40 hour per week positions. As a direct employee of one of our clients you will be provided with full benefits including Health Insurance, Vacation, Sick Pay, Relocation money and a lucrative sign-on bonus. I have supervisory, team lead and bench positions. These positions require HTL or HT certification or registry eligibility. Here are some of my HOTTEST Histology Supevisory positions: 1.Maine - Histology Supervisor 2.Upstate NY - Histology Supervisor 3.Boston, MA - Histology Supervisor Here are some of my HOTTEST Histo Tech bench positions: 1. Central Florida - Histo Tech 2. Southern Florida - Histo Tech 3. Central VA - Histo Tech 4. Northern, VA - Histo Tech 5. Southern, VA - Histo Tech 6. Pittsburgh, PA - Histo Tech 7. Baltimore area, MD - Histo Tech 8. Los Angeles area CA - Immunohistochemistry Tech 9. Oregon - Histo Tech 10. Michigan - Histo Tech 11. San Antonio area, TX - Histo Tech 12. Northern CA - Histo Tech 13.Illinois - Team Lead 14.Dayton, OH - Histo Tech If you are interested in these jobs, please CALL ME ASAP at 800 466 9919 x234. To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well. I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you. Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who do not have an email address, if you could pass my query & name on to them I'd be very grateful. I don't want to be a bother - I was told that you were a hands-on Histo Tech or a Lab Supervisor. If you are no longer working in a lab please send me an email and I will remove you from my list of people to contact. However, if you are interested in any of the jobs above, please call me. Thank You !! Pam - 800 466 9919 ext 234 --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing Learn More About Ategra: Ategra Systems Inc Specialists in Permanent & Contract Staffing VOICE: 407-671-5800 ext 234 TOLLFREE: 800-466-9919 ext 234 EMAIL: pam@ategra.com WEBSITE: --------------------------------------------------------- From bruyntjes <@t> voeding.tno.nl Fri Dec 12 08:55:18 2003 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] (no subject) Message-ID: <3B070848E7C2204F9DEB8BCFD767728001079C9D@ntexch1.voeding.tno.nl> Hi all Can someone tell me, the type of buffer you use in immunocytochemistry can it be killing for a successful staining? I mean it in the way that an antibody will react when PBS is used instead of TBS, and the other way around? Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From Nancy.Walker <@t> sanofi-synthelabo.com Fri Dec 12 09:25:26 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] =?iso-8859-1?Q?R=E9f=2E_=3A_[Histonet]_Skeletal_Histology_Reference_Books?= Message-ID: Is there anything that includes embryology? From charles.lawrie <@t> ndcls.ox.ac.uk Fri Dec 12 09:30:05 2003 From: charles.lawrie <@t> ndcls.ox.ac.uk (Charles Lawrie) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] arabinose antibody/lectin Message-ID: Dear all, Does anybody have idea where I could find an antibody (ideally) or lectin that is specific for arabinose that I could use for paraffin staining? many thanks in advance Charles Lawrie Dr. Charles H. Lawrie, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, LRF Immunodiagnostics Unit, Room 5501, Level 5, John Radcliffe Hospital, Oxford. OX3 9DU. Tel: (01865) 222197 e-mail: charles.lawrie@ndcls.ox.ac.uk From laurie.colbert <@t> huntingtonhospital.com Fri Dec 12 10:13:30 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Slides and cassettes printer/labeler Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C79@EXCHANGE1.huntingtonhospital.com> Hi Bruce, Did you ever get any feedback on cassette printers? I am interested in hearing what others have to say, too. I have checked out a couple of them. We tried the Shandon but I found it too hard to move around on the screen and get it to print what I wanted it to quickly. The accession numbers did not count up automatically so you had to manually enter the number each time. I was not able to batch-print cassettes. We tried the TBS Shurmark Plus and I absolutely loved the touch-screen and the ability to move around on the screen. Unfortunately, it took too long for the cassettes to print and two minutes for the ink to dry. That won't work for us. I had the brand new Sakura in this week, and I have mixed feelings. It is also cumbersome to move around on the screen but the print on the cassettes looks beautiful. Needless to say, I haven't fallen in love with any one labeler yet. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: Chung, Luong [mailto:lchung@ppmh.org] Sent: Tuesday, November 25, 2003 8:34 AM To: Histonet (E-mail) Subject: [Histonet] Slides and cassettes printer/labeler Histoneter, At our lab, we are looking into the slides and cassettes printer/labeler. Can anyone give me any advise or suggestion? I know that Sakura and Leica make them. Is there any other one? Pros and Cons between the two would be nice? Thanks in advance, Bruce Chung, MSM, CT(ASCP) Phoebe Putney Memorial Hospital Anatomic Pathology Manager HIPAA Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being sent to you may include PHI, after appropriate consent, acknowledgement, or authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose this patient information without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject us, or you, to penalties described in federal (HIPAA) and state law. If you, the reader of this message, are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. Thanks for your help. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Dec 12 10:46:53 2003 From: jkiernan <@t> uwo.ca (J. A. Kiernan) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] arabinose antibody/lectin References: Message-ID: <3FD9F0FD.C35238BA@uwo.ca> Arabinose is soluble in water and in alcohol (see Merck Index). How will you retain it in situ in paraffin sections? -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ------------------------- Charles Lawrie wrote: > > Dear all, > Does anybody have idea where I could find an antibody (ideally) or > lectin that is specific for arabinose that I could use for paraffin > staining? > > many thanks in advance > > Charles Lawrie > > Dr. Charles H. Lawrie, > Nuffield Department of Clinical Laboratory Sciences, > University of Oxford, > LRF Immunodiagnostics Unit, > Room 5501, Level 5, > John Radcliffe Hospital, > Oxford. OX3 9DU. > Tel: (01865) 222197 > e-mail: charles.lawrie@ndcls.ox.ac.uk > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Walker <@t> sanofi-synthelabo.com Fri Dec 12 10:47:02 2003 From: Nancy.Walker <@t> sanofi-synthelabo.com (Nancy.Walker@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] =?iso-8859-1?Q?R=E9f=2E_=3A_Re=3A_[Histonet]_more_PAS_on_ISH?= Message-ID: Yeah you're of course right.... I'm trying both the alcian blue (and the toluidine blue) so I had a little dyslexia. I raised the alcian blue conc. up to 5g/100ml but it still doesn't penetrate, (it didn't infact disolve the emulsion) but it still didn't stain so I did Toludine blue. And I guess I'll just have to start over and try what you suggested the Alcian/PAS after hybridization and before the emulsion. thanks so much, Nancy From Catherine.Goeden <@t> med.va.gov Fri Dec 12 11:04:30 2003 From: Catherine.Goeden <@t> med.va.gov (Goeden, Catherine) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] COX-2 antibody Message-ID: <6A6686E52FDAD5118CC30000F8034A25931825@VHASUXEXC1> Hello everyone in Histo-land and Happy Friday!! I am looking for a procedure using Biocare antibody for COX-2 and their detection kits. I am in need of the step by step instructions and have been having difficulty finding the procedure. Can anyone help? Thanks in advance From juan.gutierrez <@t> christushealth.org Fri Dec 12 11:49:25 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Formaldehyde Message-ID: Yes, there is at least two companies that sell powder neutralizers; Sakura and Surgipath are the first two that come to mind. I have used both with good results. Buena suerte, Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Lab Histologia [mailto:histolog@fcv.unl.edu.ar] Sent: Fri 12/12/2003 8:13 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Formaldehyde Hi All, I need to know if exists some method for to inactivate or neutralize formaldehyde. Thank you ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dobbin <@t> upei.ca Fri Dec 12 11:09:03 2003 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] COX-2 antibody In-Reply-To: <6A6686E52FDAD5118CC30000F8034A25931825@VHASUXEXC1> Message-ID: <3FD9CC00.9544.11D0C9D@localhost> Catherine, Just follow the directions included with the detection kit you are using. All you have to do is optimize the parameters surrounding the primary antibody, i.e. dilution, incubation temperature, incubation time and whether or not antigen retreival is necessary. So start with the manufacturers suggested dilution and titrate around that (eg if 1:200 is suggested for IHC, try 1:50, 1:100, 1:200, and 1:400). For incubation start with the simplest, 1 hr at room temp. If with these parameters you get no signal, try incubation overnight at 4 C (fridge). If still no signal, or siganal is too weak, try an antigen retreival method (assuming you are working with formalin fixed tissue??). For example, 20 mins. in a citrate buffer in a vegetable steamer. If after all this you still have no signal, I would try a different primary antibody, not all antibodies are created equal! I found that a polyclonal anti-COX-2 made to recognize human COX-2 worked well with my rat tissues whereas a polyclonal anti-COX-2 made to recognize canine COX-2 did not work. Now if after Ag retreival you have too much signal (ie signal on deletion control) than the problem might be endogenous biotin (retreival enhances or accentuates endogenous biotin expression). Use a biotin block after retreival but before detection kit (I apply mine before the primary antibody). I hope this wasn't too elementary for you. If I over-simplified the explanation or if I was way off base on what you were looking for, it would be due to the lack of details in your e-mail request about what you are working with or what you may have already tried. Have a nice weekend and good luck. Greg Date sent: Fri, 12 Dec 2003 11:04:30 -0600 From: "Goeden, Catherine" Subject: [Histonet] COX-2 antibody To: "'histonet@lists.utsouthwestern.edu'" > Hello everyone in Histo-land and Happy Friday!! > > I am looking for a procedure using Biocare antibody for COX-2 and their > detection kits. I am in need of the step by step instructions and have been > having difficulty finding the procedure. Can anyone help? Thanks in > advance > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Greg Dobbin Pathology Lab Atlantic Veterinary College, U.P.E.I. 550 University Ave. Charlottetown, P.E.I. Canada, C1A 4P3 Phone: (902)566-0744 Fax: (902)566-0851 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Making a living is getting; making a life is giving. From JosefaNava <@t> texashealth.org Fri Dec 12 13:24:16 2003 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Looking for CD4,CD8,EGFR.Collagen IV antibodies Message-ID: Hello Everyone, Can you tell me where I can find the following antibodies, CD4,CD8, Collagen IV and EGFR and what clone is the best , that will work with Ventana Nexes. I thank you so much. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From scoop <@t> mail.nih.gov Fri Dec 12 13:33:44 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] arabinose antibody/lectin In-Reply-To: References: Message-ID: I don't know if they have lectin specific for arabinose, but Vector labs has a bunch of lectins, including lectins coupled to HRP. Sharon >Dear all, >Does anybody have idea where I could find an antibody (ideally) or >lectin that is specific for arabinose that I could use for paraffin >staining? > >many thanks in advance > >Charles Lawrie > >Dr. Charles H. Lawrie, >Nuffield Department of Clinical Laboratory Sciences, >University of Oxford, >LRF Immunodiagnostics Unit, >Room 5501, Level 5, >John Radcliffe Hospital, >Oxford. OX3 9DU. >Tel: (01865) 222197 >e-mail: charles.lawrie@ndcls.ox.ac.uk > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From JWilkie <@t> stmarygj.com Fri Dec 12 14:30:49 2003 From: JWilkie <@t> stmarygj.com (Joey Wilkie) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] (no subject) Message-ID: <1689B20EEDC4D711B23400508BB814D5E7CAB6@smh-exchange.stmarygj.com> We are habing problems with brown precipitant on our slides after we have stained with H&E on our automated stainer. We do have a Tissue-Tek coverslipper. The pathologists say the the H&E staining is good. We called Sukura and they said that maybe our slides were not getting completely dehydrated. We have tried uping the times on our stainer, we are changing the alcohols almost after each rack and we are still getting brown precipitant. Our pathologist are saying that as they screen the slides that they can see the brown precipitant forming. When we coverslip by hand we are not getting the brown precipitant. We can use any and all suggestions that we can get Thanks, Joey Wilkie, HT St. Mary's Hospital Grand Junction, Colorado This electronic message and all contents contain information from St. Mary's Hospital which may be attorney-client privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender immediately and destroy the original message and all copies. Thank you." -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031212/e26c26b3/attachment.htm From mcauliff <@t> umdnj.edu Fri Dec 12 14:55:02 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] arabinose antibody/lectin In-Reply-To: References: Message-ID: <3FDA2B26.4000608@umdnj.edu> Talk to Vector Labs in Burlingame, CA. They are lectin experts. Geoff Charles Lawrie wrote: >Dear all, >Does anybody have idea where I could find an antibody (ideally) or >lectin that is specific for arabinose that I could use for paraffin >staining? > >many thanks in advance > >Charles Lawrie > >Dr. Charles H. Lawrie, >Nuffield Department of Clinical Laboratory Sciences, >University of Oxford, >LRF Immunodiagnostics Unit, >Room 5501, Level 5, >John Radcliffe Hospital, >Oxford. OX3 9DU. >Tel: (01865) 222197 >e-mail: charles.lawrie@ndcls.ox.ac.uk > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From funderwood <@t> mcohio.org Fri Dec 12 14:52:29 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] (no subject) Message-ID: Maybe you are not having enough xylene dispensed onto the slide during coverslipping. Fred >>> Joey Wilkie 12/12/03 03:30PM >>> We are habing problems with brown precipitant on our slides after we have stained with H&E on our automated stainer. We do have a Tissue-Tek coverslipper. The pathologists say the the H&E staining is good. We called Sukura and they said that maybe our slides were not getting completely dehydrated. We have tried uping the times on our stainer, we are changing the alcohols almost after each rack and we are still getting brown precipitant. Our pathologist are saying that as they screen the slides that they can see the brown precipitant forming. When we coverslip by hand we are not getting the brown precipitant. We can use any and all suggestions that we can get Thanks, Joey Wilkie, HT St. Mary's Hospital Grand Junction, Colorado This electronic message and all contents contain information from St. Mary's Hospital which may be attorney-client privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender immediately and destroy the original message and all copies. Thank you." From asmith <@t> mail.barry.edu Fri Dec 12 15:34:19 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] Formaldehyde Message-ID: <494304423C63E246A5CF87A3AEEB577011B5B4@bumail01.barrynet.barry.edu> I have been quite happy with VYTAC formalin neutralizer from Richard Allen Scientific (225 Parsons St., Kalamazoo, Michigan, USA). Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Lab Histologia Sent: Friday, December 12, 2003 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde Hi All, I need to know if exists some method for to inactivate or neutralize formaldehyde. Thank you ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From laurie.colbert <@t> huntingtonhospital.com Fri Dec 12 15:49:07 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] (no subject) Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C7B@EXCHANGE1.huntingtonhospital.com> The coverslipping film is not completely adhering to the slides because: 1) There's not enough xylene being dispensed onto the slides and the slides are drying out (it's better to dispense too much xylene than not enough) 2) The white roller that helps "push" the film down onto the slide is not in the "down" position 3) The tissue is too thick. We get this more often on decal slides than other slides. Laurie Colbert Pasadena, CA -----Original Message----- From: Joey Wilkie [mailto:JWilkie@stmarygj.com] Sent: Friday, December 12, 2003 12:31 PM To: 'HistoNet@pathology.swmed.edu' Subject: [Histonet] (no subject) We are habing problems with brown precipitant on our slides after we have stained with H&E on our automated stainer. We do have a Tissue-Tek coverslipper. The pathologists say the the H&E staining is good. We called Sukura and they said that maybe our slides were not getting completely dehydrated. We have tried uping the times on our stainer, we are changing the alcohols almost after each rack and we are still getting brown precipitant. Our pathologist are saying that as they screen the slides that they can see the brown precipitant forming. When we coverslip by hand we are not getting the brown precipitant. We can use any and all suggestions that we can get Thanks, Joey Wilkie, HT St. Mary's Hospital Grand Junction, Colorado This electronic message and all contents contain information from St. Mary's Hospital which may be attorney-client privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender immediately and destroy the original message and all copies. Thank you." -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031212/7f1adc4a/attachment.htm From weneng <@t> hotmail.com Fri Dec 12 16:37:07 2003 From: weneng <@t> hotmail.com (Wendy England) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] salary survey Message-ID: Hi histonetters: Could anyone send me the website address for salary survey please? Many thanks! Wendy _________________________________________________________________ Wonder if the latest virus has gotten to your computer? Find out. Run the FREE McAfee online computer scan! http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From scoop <@t> mail.nih.gov Fri Dec 12 16:49:38 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] cacodylate vs PBS Message-ID: Hi, histonetters. There is a debate in my lab regarding which buffer is best for IHC on FFPE tissue. I use 0.1M PBS made up the usual way (Sorensen's?) Other people in my lab use NaCacodylate. I have been told by various people that NaCacodylate is used for some EM purposes and doesn't preserve antigenicity or isn't the best choice for IHC for some other reason. Does anyone have info or an opinion on this to either set me straight or provide me with ammunition for the next round of arguing? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From scoop <@t> mail.nih.gov Fri Dec 12 16:53:13 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] fluoro-jade B Message-ID: Has anyone tried Fluoro-Jade and had it work? I've found web sites (including the manufacturer's) that show great pictures, but everyone I've ever talked to who tried it says it doesn't work (many more people say it doesn't work than say it does. I want to try it on my FFPE mouse brains, but I don't want to waste a lot of time if it's hopeless. Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From SimonSm <@t> vulcan.com Fri Dec 12 18:05:49 2003 From: SimonSm <@t> vulcan.com (Simon Smith (Temporary)) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] unsubscribe Message-ID: <48C7ABE775B5494CB235A04A0325D1310E28@darwin.corp.vnw.com> Simon Smith Research Associate III Allen Institute for Brain Science Phone: (206) 548 7021 Fax: (206) 548 7071 simonsm@vulcan.com www.brainatlas.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031212/4fe67286/attachment.htm From lpwenk <@t> covad.net Fri Dec 12 19:28:30 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:20 2005 Subject: [Histonet] salary survey References: Message-ID: <006f01c3c118$6af4a660$f728a544@hppav> http://www.ascp.org/bor/center/center_research.asp The "space" between center and research is an underline - center_research I can't figure out how to get the computer to let me write the address without the underline. Peggy A. Wenk ----- Original Message ----- From: "Wendy England" To: Sent: Friday, December 12, 2003 5:37 PM Subject: [Histonet] salary survey > Hi histonetters: > > Could anyone send me the website address for salary survey please? Many > thanks! > > Wendy > > _________________________________________________________________ > Wonder if the latest virus has gotten to your computer? Find out. Run the > FREE McAfee online computer scan! > http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CrochiereSteve <@t> aol.com Fri Dec 12 22:51:01 2003 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Job Opening in Central Mass. Message-ID: <71.38c875c7.2d0bf4b5@aol.com> Hi Histo Net, I have word of a sweet position in a smaller hospital in Southbridge, MA. You'd be working for 2 of the nicest pathologists I ever worked for. (I never should have left them, but got lured away by the dark side of pharm. which failed miserably, by the way) If you'd like a few details and contact information, please reply to this e-mail and I can set you up with an interview. Steven M. Crochiere, HT (ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center 299 Carew St. Springfield, MA 01104 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031212/3a0c4713/attachment.htm From jkiernan <@t> uwo.ca Fri Dec 12 23:30:33 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] fluoro-jade B References: Message-ID: <3FDAA3F9.66CCE012@uwo.ca> Dead cells are acidophilic (= they stain strongly with anionic dyes). That includes fluorescent anionic dyes such as the eosins and acid fuchsine. There's plenty of respectable peer-reviewed literature about all this, especially for dead neurons. The patents that refer to fluoro-jade describe it as something similar to eosin Do some googling! Look up the papers, and you will probably find that there is no need to buy an unidentified stain for dead neurons. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ----------------------------------------- Sharon Cooperman wrote: > > Has anyone tried Fluoro-Jade and had it work? I've found web sites > (including the manufacturer's) that show great pictures, but everyone > I've ever talked to who tried it says it doesn't work (many more > people say it doesn't work than say it does. I want to try it on my > FFPE mouse brains, but I don't want to waste a lot of time if it's > hopeless. > > Sharon > -- > Sharon Cooperman > NIH, NICHD, CBMB 301.435-7735 > Building 18T, room 101 301.402-0078 fax > Bethesda, MD 20892 ---------------------------------------------------------- From dteleis <@t> vet.upenn.edu Sat Dec 13 06:17:35 2003 From: dteleis <@t> vet.upenn.edu (Donna Teleis) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] L.A.B. Solution Message-ID: <3FD1A1D7@webmail.vet.upenn.edu> We used the LAB solution recently for IGF-1, and it worked well at 10 min, RT. Thetissue was fixed in formalin and embedded in both parafin and MMA. The MMA sections look like they may benefit from longer exposure, but we did have some staining. >===== Original Message From ===== >Hi Histonetters, >Has anyone ever tried Polysciences' Liberate Antibody Binding (LAB) >Solution? The product (www.polysciences.com Cat.No. 24310) is supposed to >exposure the antigenic site of the FFPE tissue sections by incubating at >room temperature for 5-20 minutes, eliminating the used of heating (HIER)!! >This would give enormous changes the way we do immunostaining so far. > >Among few antibodies I tried so far, only LCA (CD45) and CD20 (L-26) were >good enough and Progesterone receptor showed a weak staining. > > >Regards > > > >Young Kwun >Senior Hospital Scientist >Dept. of Anatomical Pathology >Concord Hospital >Concord NSW 2139 Australia >Tel)61-2-9767-6075 >Fax)61-2-9767-8427 >kwuny@email.cs.nsw.gov.au > >"the universe we observe has precisely the properties we should expect if >there is, at bottom, no design, no purpose, no evil and no good, nothing but >blind, pitiless indifference." >"To err is human, to forgive is even more human" > >The information contained in this message is intended for the named >addressee only, and is confidential to the sender and intended recipient. If >you are not the named addressee please do not copy, distribute, take any >action reliant on, or disclose anything in this E-mail message to any other >person or organisation. If you have received this message in error please >delete the email and notify me immediately. Views expressed in this message >are those of the individual sender and are not necessarily the views of >Central Sydney Area Health Service. > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Donna Teleis Research Specialist Department of Clinical Studies Equine Sports Medicine Building University of Pa., New Bolton Center phone: 610-925-6429 fax: 610-925-8131 From rschoon <@t> email.unc.edu Sat Dec 13 11:29:11 2003 From: rschoon <@t> email.unc.edu (rschoon@email.unc.edu) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] fluoro-jade B In-Reply-To: References: Message-ID: <1071336551.3fdb4c678da3a@webmail8.isis.unc.edu> Sharon, I did this stain when I worked at a private Tox-Path lab. about 6 months ago. It works fine following the directions given on the web site...however as with all stains a good positive control is critical. i don't have micrographs as this was done at my old part-time job and everything I did there, stays there. I also agree with John Kiernan it is a dye of unkown composition and is very expensive for what you get. Robert Schoonhoven Quoting Sharon Cooperman : > Has anyone tried Fluoro-Jade and had it work? I've found web sites > (including the manufacturer's) that show great pictures, but everyone > > I've ever talked to who tried it says it doesn't work (many more > people say it doesn't work than say it does. I want to try it on my > > FFPE mouse brains, but I don't want to waste a lot of time if it's > hopeless. > > Sharon > -- > Sharon Cooperman > NIH, NICHD, CBMB 301.435-7735 > Building 18T, room 101 301.402-0078 fax > Bethesda, MD 20892 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dave_laidley <@t> yahoo.ca Sat Dec 13 13:03:04 2003 From: dave_laidley <@t> yahoo.ca (David Laidley) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Divisions of hippocampus Message-ID: <20031213190304.92662.qmail@web20804.mail.yahoo.com> Could anyone recommend a website, paper or text that clearly show the divisions of the hippocampus in a rodent. Most specficially, I would like to know where the boundries between CA1 and subiculum exist in coronal sections. --------------------------------- Post your free ad now! Yahoo! Canada Personals -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031213/6c7e2750/attachment.htm From Rcartun <@t> harthosp.org Sun Dec 14 11:07:14 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] HCV testing Message-ID: Does anyone perform molecular testing for hepatitis C virus on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Cartun From caroline.stott <@t> anatomy.otago.ac.nz Sun Dec 14 14:52:12 2003 From: caroline.stott <@t> anatomy.otago.ac.nz (Caroline Stott) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Joey Wilkie (no subject) In-Reply-To: <1689B20EEDC4D711B23400508BB814D5E7CAB6@smh-exchange.stmary gj.com> Message-ID: <5.2.1.1.0.20031215094908.0258c030@anatomy.otago.ac.nz> Have you been filtering the haematoxylin before use? Also, what haematoxylin are you using? We use Harris haematoxylin and differentiate the slides in 0.5% acid/alcohol (conc HCL in 70% alc) for 10 seconds. These steps should eliminate any precipitate. Caroline At 09:30 13/12/03 +1300, you wrote: >We are habing problems with brown precipitant on our slides after we have >stained with H&E on our automated stainer. We do have a Tissue-Tek >coverslipper. The pathologists say the the H&E staining is good. We >called Sukura and they said that maybe our slides were not getting >completely dehydrated. We have tried uping the times on our stainer, we >are changing the alcohols almost after each rack and we are still getting >brown precipitant. Our pathologist are saying that as they screen the >slides that they can see the brown precipitant forming. When we coverslip >by hand we are not getting the brown precipitant. We can use any and all >suggestions that we can get > >Thanks, Joey Wilkie, HT >St. Mary's Hospital >Grand Junction, Colorado >This electronic message and all contents contain information from St. >Mary's Hospital which may be attorney-client privileged, confidential or >otherwise protected from disclosure. The information is intended to be >for the addressee only. If you are not the addressee, any disclosure, >copy, distribution or use of the contents of this message is >prohibited. If you have received this electronic message in error, please >notify the sender immediately and destroy the original message and all >copies. Thank you." Caroline Stott Histology Service Unit Medical School University of Otago Dunedin (03) 479 7152 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031215/33f18aca/attachment.htm From AnthonyH <@t> chw.edu.au Sun Dec 14 16:11:04 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] ACE Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E102@simba.kids> You could try this: Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html ACETYLCHOLINESTERASE Principle: The diagnosis of Hirschsprung's disease is by showing the absence of ganglion cells in the distal portion of the large intestine. Hirschsprung's disease may be divided into 3 main groups: The first and most common, comprises those patients with aganglionosis as far as the rectosigmoid junction (short segment disease). The second group of patients has aganglionosis extending beyond the rectosigmoid junction but not involving the small bowel (long segment disease). The third group which accounts for 2-14% of all Hirschsprung's disease patients have aganglionosis extending into the small bowel, sometimes as far as the duodenum or stomach (total colonic aganglionosis). In both long and short segment disease the appearance in the Acetylcholinesterase preparations will show an increase in nerve fibres present in the muscularis mucosae and this is accompanied by an absence of ganglion cells and an increase in nerve fibres and nerve trunks in the submucosa. This general picture varies with the age of the patient and many clinical factors need to be taken into consideration before a conclusion can be reached. Section Requirements: Cryostat sections (5-6 m) on poly-L-lysine slides Cut sections at 5m and stain with toluidine blue. If the appropriate level is obtained then cut 10 sections for the AChE stain. Cut and stain another tol blue at the end. (To be discussed with the Pathologist) Solutions: 1. 10% buffered formalin cooled to 4oC 2. Dilute Ammonium Sulphide (about 0.005%) 20% Ammonium sulphide 25 ul Tap water 50 ml 3. 0.1% Silver Nitrate 4. Incubation Medium A. Make up 648m/ (for 72 tubes) of medium A: Acetylthiocholine iodide 3640mg Sodium Acetate (0.06M) 3.719 g Acetic Acid (0.1M) 0.082ml Sodium Citrate (0.1M) 1.058 g Copper Sulphate (0.03M) 0.540 g Add distilled water up to 633.7ml OMPA (Tetraisopropyl Pyrophosphoramide) (0.004M) 0.020g Aliquot medium A Into 9ml tubes and store at -25oC 5. Incubation Medium B Make up 72ml of medium B (for 72 tubes): Potassium - ferricyanide 5mM (0.005M) 0.119g/72ml distilled water. Aliquot into 1ml tubes and store at -25? C. Incubation solution: After defrosting, mix one vial of both Incubation medium A and B. Method: 1. Air dry sections for 15 minutes 2. Fix sections in formol-saline for 10 minutes (at 4?C) 3. Wash well with distilled water 4. Incubate in substrate solution at 37?C for 60 minutes (mix A+B) 5. Wash briefly in distilled water 6. Treat with dilute ammonium sulphide for 30 seconds ( in fume hood) 7. Wash in tap water then rinse in distilled water 8. Treat with 0.1% silver nitrate for 1 minute at room temperature 9. Wash in distilled water 10. Counterstain with haematoxylin for 30 seconds 11. Rinse slides in water 12. Dip in blueing solution for 1 minute 13. Rinse quickly in distilled water 14. Dehydrate, clear and mount Results: Nerve fibres, ganglion cells, and other tissue containing acetylcholinesterase are stained dark brown to black. (Beware - erythrocyte membranes also contain endogenous acetylcholinesterase). References: Karnovsky and Roots, J. Histochemistry and Cytochemistry, 1964, Vol 12, p 219-221. Filipe and Lake, Histochemistry in Pathology, 2nd Ed, 1990, p463. -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@covad.net] Sent: Friday, 12 December 2003 9:22 PM To: Histonet Cc: Sharon Scalise Subject: [Histonet] ACE Need help with the ACE (acetylcholine esterase) procedure for Hirshsprung Disease. Two choices: 1. Can someone tell me a source of ethopropazine? We are no longer able to obtain it through Sigma/Aldrich. We did order it from another company, but now cannot get the stain to work. OR 2. Can someone share with me their procedure, which does not include this chemical. Thank you. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Dec 14 16:29:21 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Formaldehyde Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E103@simba.kids> John A. Kiernan posted this message to Histonet back in 1999. I kept a copy of it. Very usefull Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html John A. Kiernan writes: Here is a repeat of a message I sent to HistoNet about a year ago, replying to a related question. It relates only to formalin, and a one-line summary would be: Neutralize it with ammonia. There is a reference to a book about formaldehyde. Neutralization of formalin with ammonia ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 6CH2O + 4NH3 = C6H12N4 + 6H2O 6 * 30.03 4 * 17.03 = 180.18 = 68.12 140.19 grams 180 g CH2O are contained in 180/0.04 = 4500 ml of 4% (=0.04g/ml) solution (10% formalin) 68 g NH3 are conmtained in 68/0.27 = 252 ml of 27% (=0.27g/ml) solution (strong ammonia) Therefore 1000 ml of 10% formalin (= 4% formaldehyde) reacts with 56 ml of strong ammonia solution, generating 31 g of hexamine (in an approximately 3% solution). The MSDS for hexamine has all the usual dire warnings, relating to the solid. Various LD50s range from 250 to 9500 mg/kg. The sheet for hexamine mandelate (the salt used therapeutically as a urinary antiseptic) contains much less information, and doesn't have nearly so much in it. Hexamine and its salts are slowly hydrolysed, releasing formaldehyde, and this reaction, which is speeded up by acids, is probably the basis of the antiseptic properties. The proper name for hexamine is hexamethylenetetramine, and it's also commonly called methenamine. The Merck Index lists many other synonyms. For more information see Walker,JF (1964) Formaldehyde. 3rd ed. New York: Reinhold and London: Chapman & Hall. What I do is add about 50 ml of strong ammonia to a container with a specimen in a litre or so of formalin fixative, but the lid back on, give the pot a swirl, and wait for half an hour or so (the reaction is much faster, so the wait probably isn't really needed.) The smell of formalin is greatly reduced or replaced by a faint whiff of ammonia. John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 E-mail: kiernan@uwo.ca -----Original Message----- From: Lab Histologia [mailto:histolog@fcv.unl.edu.ar] Sent: Saturday, 13 December 2003 1:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde Hi All, I need to know if exists some method for to inactivate or neutralize formaldehyde. Thank you ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA Tel. (54)3496-420639 Fax. (54)3496-426304 http://fcv.unl.edu.ar/histolog/ http://fcv.unl.edu.ar/bioterio/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Dec 14 21:34:39 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] RE: IHC on cells on coverslips Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E105@simba.kids> Alexander, Yes, granted S100 is alcohol soluble, but for melanoma confirmation, one can use HMB-45 or Melan-A. Remember, sometimes one may only have one or two cell smears available in order to confirm a diagnosis (eg no material in the cell block) so one must resort to one of the available smears. In this instance, collecting evidence and expertise is important. We need to appreciate the usefulness and shortcommings of the antibodies we use. It does seem that there are more antibodies that work on ethanol fixed smears than those that do not. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Nader, Alexander [mailto:alexander.nader@wgkk.sozvers.at] Sent: Friday, 12 December 2003 11:36 PM To: Histology Net List Server (E-Mail) Subject: [Histonet] RE: IHC on cells on coverslips > "Not really beneficial to many antigens"? I could list at least 50 > antibodies that work on ethanol fixed smears. Most of these > are in routine > use. > eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA, > Skeletal muscle myosin, Actin,etc etc. > S100-Antigen, GCFBP and some other ab's "leach out" with ethanol fixation, even fixation of trephines with Schaffer's solution (ethanol/formalin mixture) shows this unwanted and rather nasty effect. Alexander Nader MD Vienna, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From zumbor <@t> EMAIL.CS.NSW.GOV.AU Sun Dec 14 21:35:46 2003 From: zumbor <@t> EMAIL.CS.NSW.GOV.AU (Rosalba) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] NeuN Message-ID: <00bb01c3c2bc$89080430$1e7b4c98@HistoLab> Hi, Has anyone had any experience with the NeuN antibody on formalin fixed paraffin tissue. Thanks Rosalba -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031215/cf704e59/attachment.htm From ronakorn_p <@t> yahoo.com Mon Dec 15 01:13:43 2003 From: ronakorn_p <@t> yahoo.com (Ronakorn Panjaphongse) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] The best staining method for new bone formation Message-ID: <20031215071343.66436.qmail@web41203.mail.yahoo.com> Hello everybody I would like to conduct the study to evaluate the action of Bone Morphogenetic Protein (BMP) antagonist in prevent new bone formation. But the well known H&E stain may be not good enough because both matrix and new bone formation ( osteoid ) is also red, and it is also very hard in quantitative evaluation by using computer program ,eg. NIH too. Does anybody has some advice in better staining method or other way in quantitative evaluation that able to differentiate the new bone formation from the peripheral matrix. Thank you very much for your answer in advance. Ronakorn Panjaphongse Graduate Student Graduate School of Biomedical Science Hiroshima University Japan. --------------------------------- Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031214/aedebf2c/attachment.htm From IKirbis <@t> onko-i.si Mon Dec 15 01:47:18 2003 From: IKirbis <@t> onko-i.si (Kirbis Srebotnik Irena) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] RE: IHC on cells on coverslips Message-ID: however, we got nice S-100 positive staining on many many Papanicolaou stained smears and cytospins. we use S-100 on Papanicolaou stained slides since 1990! Irena Kirbis Cytopathology dept -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: Monday, December 15, 2003 4:35 AM To: Histology Net List Server (E-Mail) Cc: 'Nader, Alexander' Subject: RE: [Histonet] RE: IHC on cells on coverslips Alexander, Yes, granted S100 is alcohol soluble, but for melanoma confirmation, one can use HMB-45 or Melan-A. Remember, sometimes one may only have one or two cell smears available in order to confirm a diagnosis (eg no material in the cell block) so one must resort to one of the available smears. In this instance, collecting evidence and expertise is important. We need to appreciate the usefulness and shortcommings of the antibodies we use. It does seem that there are more antibodies that work on ethanol fixed smears than those that do not. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Nader, Alexander [mailto:alexander.nader@wgkk.sozvers.at] Sent: Friday, 12 December 2003 11:36 PM To: Histology Net List Server (E-Mail) Subject: [Histonet] RE: IHC on cells on coverslips > "Not really beneficial to many antigens"? I could list at least 50 > antibodies that work on ethanol fixed smears. Most of these > are in routine > use. > eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA, > Skeletal muscle myosin, Actin,etc etc. > S100-Antigen, GCFBP and some other ab's "leach out" with ethanol fixation, even fixation of trephines with Schaffer's solution (ethanol/formalin mixture) shows this unwanted and rather nasty effect. Alexander Nader MD Vienna, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031215/08545659/attachment.htm From c.m.vanderloos <@t> amc.uva.nl Mon Dec 15 04:58:00 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] RE: PBS or TBS? Message-ID: <34e03d351118.35111834e03d@amc.uva.nl> Dear Joost, As far as I know there is no difference in using PBS or TBS as washing buffer. Certainly not in terms of "killing" as you wrote. IHC is a very tolerant technique in this respect! The only situation to be careful with is when using alkaline phosphatase as marker enzyme. The enzymatic activity of this enzyme is heavily inhibited by phosphate ions in PBS. In this case you have to rinse your slides 3 times with a non-phosphate containing buffer (Tris-buffer for example) before starting the AP visualization reaction. Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands ----- Original Message ----- >From "Bruijntjes, J.P." Date Fri, 12 Dec 2003 15:55:18 +0100 To Subject [Histonet] (no subject) Hi all Can someone tell me, the type of buffer you use in immunocytochemistry can it be killing for a successful staining? I mean it in the way that an antibody will react when PBS is used instead of TBS, and the other way around? Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands From Stephen.Eyres <@t> sanofi-synthelabo.com Mon Dec 15 06:07:57 2003 From: Stephen.Eyres <@t> sanofi-synthelabo.com (Stephen.Eyres@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] fluoro-jade B Message-ID: Hi Sharon, I've used FJB and it worked very well. Having read John Kiernan's eosin comments, tried out solution too. It is work but the results were not as clear. However, I did not try to optimise the eosin method used. Cheers Steve Sharon Cooperman To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] fluoro-jade B western.edu 12/12/2003 22:53 Has anyone tried Fluoro-Jade and had it work? I've found web sites (including the manufacturer's) that show great pictures, but everyone I've ever talked to who tried it says it doesn't work (many more people say it doesn't work than say it does. I want to try it on my FFPE mouse brains, but I don't want to waste a lot of time if it's hopeless. Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Mon Dec 15 07:46:25 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] NeuN Message-ID: Rosalba, We use Chemicon's NeuN at 1:200 after HIER in 1mM EDTA. If you need more info. feel free to contact me. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Clinical & Research Laboratory DM223-VA 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Rosalba [mailto:zumbor@EMAIL.CS.NSW.GOV.AU] Sent: Sunday, December 14, 2003 9:36 PM To: Histonet Subject: [Histonet] NeuN Hi, Has anyone had any experience with the NeuN antibody on formalin fixed paraffin tissue. Thanks Rosalba -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031215/c46aca60/attachment.htm From rashmi <@t> neuro.duke.edu Mon Dec 15 09:43:59 2003 From: rashmi <@t> neuro.duke.edu (Rashmi Chandra) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] mouse embryo section tears Message-ID: <5.2.1.1.0.20031215103325.00a91798@neuro.duke.edu> I am sectioning paraffin embedded mouse embryos (fixed in 4% paraformaldehyde) at 7 microns. The sections cut and ribbon fine but after i dry them on the slide, tears appear in the liver. These tears are not present when I first check the sections after lifting them from the water bath (temperature 37 C) onto the slide. I think the tears may result from differential drying of the various tissues, and I am looking for a way to prevent them. Thanks a lot for your advice! Rashmi __________________________ Rashmi Chandra, Ph.D. Dept. of Neurobiology Duke University Medical Center Durham, NC 27710 Tel: (919) 681-5449 From sladd <@t> hsc.usf.edu Mon Dec 15 10:16:59 2003 From: sladd <@t> hsc.usf.edu (Sharron Ladd) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] mouse embryo section tears In-Reply-To: <5.2.1.1.0.20031215103325.00a91798@neuro.duke.edu> References: <5.2.1.1.0.20031215103325.00a91798@neuro.duke.edu> Message-ID: <3FDDDE7B.60106@hsc.usf.edu> I just did a bunch of mouse embryos (and placentas). I asked the researcher to fix them in Bouins fixative ( for less than 24 hours) since I had read that this was the best (non-mercury) fixative for embryos. I also asked that the researcher start dehydration at 30% alcohol and give them to me in 70% alcohol. The embryos cut like butter. Bouins fix will lyse red blood cells and those red cells/blood might be what is causing you problems in the liver area? I wonder if what you are calling tears is actually "chatter"? Are you soaking the block in ice water before sectioning? This might alleviate your problem. I also cut mine at 7 microns but I didn't have to soak the block and I did not have any problems with chatter. Obviously, going back and changing the fixative is not an option, but maybe next time you could try Bouins? Sharron University of South Florida Rashmi Chandra wrote: > I am sectioning paraffin embedded mouse embryos (fixed in 4% > paraformaldehyde) at 7 microns. The sections cut and ribbon fine but > after i dry them on the slide, tears appear in the liver. These tears > are not present when I first check the sections after lifting them > from the water bath (temperature 37 C) onto the slide. I think the > tears may result from differential drying of the various tissues, and > I am looking for a way to prevent them. > > Thanks a lot for your advice! > > Rashmi > __________________________ > Rashmi Chandra, Ph.D. > Dept. of Neurobiology > Duke University Medical Center > Durham, NC 27710 > > Tel: (919) 681-5449 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcauliff <@t> umdnj.edu Mon Dec 15 10:27:07 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] cacodylate vs PBS In-Reply-To: References: Message-ID: <3FDDE0DB.2040307@umdnj.edu> Hi Sharon: Rather than believe other's opinions (unless they have references to back them up) the only real way to answer the question is to 'do the experiment' yourself. The best "ammunition" is real-world results in your lab with your antigens. Sodium cacodylate has an advantage as a buffer for EM fixation in that you can dissolve calcium ions in it (for improved membrane fixation). Calcium phosphate, on the other hand, is very insoluble (this fact does not seem to deter some folks from putting Ca ions in their phosphate-buffered fix, they happily filter out the precipitate and continue on their merry way). Cacolylate has arsenic in it and should be disposed of with much greater care than phosphate buffers. Cacodylate does seem to last longer before molds begin to grow in it. After enduring the rigors of paraffin processing, I would be surprised if the choice of buffer made much difference, but it might, depending on the antigen in question. Geoff Sharon Cooperman wrote: > Hi, histonetters. There is a debate in my lab regarding which buffer > is best for IHC on FFPE tissue. I use 0.1M PBS made up the usual way > (Sorensen's?) Other people in my lab use NaCacodylate. I have been > told by various people that NaCacodylate is used for some EM purposes > and doesn't preserve antigenicity or isn't the best choice for IHC for > some other reason. Does anyone have info or an opinion on this to > either set me straight or provide me with ammunition for the next > round of arguing? > > Thanks, > Sharon -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From georgecole <@t> ev1.net Mon Dec 15 11:01:26 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Disposing of formaldehyde Message-ID: <000a01c3c32d$142b1be0$054dbad0@hppav> Mary North---muscle and nerve lady at OHSU There was a question asked a short time ago about getting rid of formaldehyde. I know the lab there at OHSU has a jug full of something that reduces formaldehyde to safe stuff to discard. I would like to tell the folks asking about that what it is. georgecole@ev1.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031215/f6d256d8/attachment.htm From scoop <@t> mail.nih.gov Mon Dec 15 11:33:29 2003 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] cacodylate vs PBS In-Reply-To: <3FDDE0DB.2040307@umdnj.edu> References: <3FDDE0DB.2040307@umdnj.edu> Message-ID: Hi. Actually, I've tried both and my best IHC and tissue quality seems to be with PBS, but I think that may be because I started with NaCacodylate and switched to PBS later and my techniques got better. I'll try Cacodylate for the antibodies I use against membrane proteins to see if that works better than PBS. Thanks, Sharon >Hi Sharon: > > Rather than believe other's opinions (unless they have references >to back them up) the only real way to answer the question is to 'do >the experiment' yourself. The best "ammunition" is real-world >results in your lab with your antigens. Sodium cacodylate has an >advantage as a buffer for EM fixation in that you can dissolve >calcium ions in it (for improved membrane fixation). Calcium >phosphate, on the other hand, is very insoluble (this fact does not >seem to deter some folks from putting Ca ions in their >phosphate-buffered fix, they happily filter out the precipitate and >continue on their merry way). Cacolylate has arsenic in it and >should be disposed of with much greater care than phosphate buffers. >Cacodylate does seem to last longer before molds begin to grow in it. > After enduring the rigors of paraffin processing, I would be >surprised if the choice of buffer made much difference, but it >might, depending on the antigen in question. >Geoff > >Sharon Cooperman wrote: > >>Hi, histonetters. There is a debate in my lab regarding which >>buffer is best for IHC on FFPE tissue. I use 0.1M PBS made up the >>usual way (Sorensen's?) Other people in my lab use NaCacodylate. >>I have been told by various people that NaCacodylate is used for >>some EM purposes and doesn't preserve antigenicity or isn't the >>best choice for IHC for some other reason. Does anyone have info >>or an opinion on this to either set me straight or provide me with >>ammunition for the next round of arguing? >> >>Thanks, >>Sharon > > >-- >-- >********************************************** >Geoff McAuliffe, Ph.D. >Neuroscience and Cell Biology >Robert Wood Johnson Medical School >675 Hoes Lane, Piscataway, NJ 08854 >voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu >********************************************** -- Sharon Cooperman NIH, NICHD, CBMB 301.435-7735 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From Barry.R.Rittman <@t> uth.tmc.edu Mon Dec 15 11:53:08 2003 From: Barry.R.Rittman <@t> uth.tmc.edu (Barry R Rittman) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] The best staining method for new bone formation Message-ID: <566FB0B522443D43AF02D2ADBE35A6F077FB98@UTHEVS3.mail.uthouston.edu> Hi - happy holidays. I would recommend that you use a trichrome such as Masson's trichrome when osteoid is stained green and bone red - this should allow easy differentiation for image analysis. Not sure if you are already doing this but it is customary to not only count the thickness of osteoid but also the numbers of osteoblasts, bone lining cells and osteoclasts per unit length. Barry -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Ronakorn Panjaphongse Sent: Monday, December 15, 2003 1:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The best staining method for new bone formation Hello everybody I would like to conduct the study to evaluate the action of Bone Morphogenetic Protein (BMP) antagonist in prevent new bone formation. But the well known H&E stain may be not good enough because both matrix and new bone formation ( osteoid ) is also red, and it is also very hard in quantitative evaluation by using computer program ,eg. NIH too. Does anybody has some advice in better staining method or other way in quantitative evaluation that able to differentiate the new bone formation from the peripheral matrix. Thank you very much for your answer in advance. Ronakorn Panjaphongse Graduate Student Graduate School of Biomedical Science Hiroshima University Japan. _____ Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031215/c2de03d3/attachment.htm From NSEARCY <@t> swmail.sw.org Mon Dec 15 12:43:18 2003 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Mohs Tech Job Description Message-ID: <03Dec15.124351cst.86670@healthcare.sw.org> Does anyone have a job description that they would care to share? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Temple, Texas Division Manager, Anatomic Pathology 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita END:VCARD From kerney <@t> fas.harvard.edu Mon Dec 15 13:37:01 2003 From: kerney <@t> fas.harvard.edu (Ryan Kerney) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Best staining method for bone Message-ID: <0DF30439-2F36-11D8-94C1-000A95BBC3D8@fas.harvard.edu> Tries Mason's trichrome. Check the web. From gentras <@t> vetmed.auburn.edu Mon Dec 15 14:00:55 2003 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Gallyas Silver Technique Message-ID: <5.2.0.9.0.20031215135845.00a39860@mailhost.vetmed.auburn.edu> Hello, will someone familiar with Gallyas Silver Technique provide me with a protocol and pointers? Thanks! Atoska Atoska S. Gentry B.S., HT(ASCP) Research Assistant III Scott-Ritchey Research Center College of Veterinary Medicine Auburn University, AL 36849 Phone# (334)844-5579 Fax# (334)844-5850 From AnthonyH <@t> chw.edu.au Mon Dec 15 15:05:29 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] RE: IHC on cells on coverslips Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E107@simba.kids> Patti, No I don't. HIER is used to reverse the effects of formalin fixation. Since I rarely use formalin to fix my cytology smears (usually use 95% ethanol or Methacarn)I do not use HIER on these smears. Having said that, I suspect that there may be antigens out there that require HIER and this is not due to the formalin fixation effect. I have not come across these yet. The question arises how antibodies to these type of antigens were raised. Were the antigens heated (similarly to HIER) prior to immunisation? Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Tuesday, 16 December 2003 4:02 AM To: Tony Henwood Subject: Re: [Histonet] RE: IHC on cells on coverslips Tony, I have a quick question for you. When you are doing IHC on ethanol fixed smears - do you use any type of antigen retrieval? I would like to optimize our IHC on smears, and am looking for some info. Thanks. Patti Loykasek Phenopath Laboratories Seattle, WA > Chris, > > "Not really beneficial to many antigens"? I could list at least 50 > antibodies that work on ethanol fixed smears. Most of these are in routine > use. > eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA, > Skeletal muscle myosin, Actin,etc etc. > > Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > http://www.histosearch.com/homepages/TonyHenwood/default.html > http://us.geocities.com/tonyhenwoodau/index.html > > > -----Original Message----- > From: C.M. van der Loos [mailto:c.m.vanderloos@amc.uva.nl] > Sent: Thursday, 11 December 2003 6:52 PM > To: Histonet@pathology.swmed.edu > Cc: pruegg@colobio.com; anthonyh@chw.edu.au > Subject: RE: IHC on cells on coverslips > > > Dear Patsy, > Please keep in mind that ethanol fixation as suggested by Tony Henwood, is > very good for the cellular morphology, but not really beneficial to many > epitopes/antigens! The acetone-fixation only works well for antigens at the > cell surface or structures that are at least tightly bound to anything. If > you are dealing with an antigen that may leak out (cytokines, hormones, grow > factors, or any other small peptide) you need a 4% PFA fixation. Because > cells grown on coverslips do have an intact outer membrane (and are not > leaking!) the antigen of interest gets nicely fixed inside the cell. You > need to add 0.1% saponin to all your reagents and buffers (from endogenous > PO block up to chromogen step) to open up the cell membrane getting your > reagents in and out. > > Chris van der Loos > Dept. of Cardiovascular Pathology > Academical Medical Center > Amsterdam - The Netherlands > > -----Original Message----- > From: Patsy Ruegg [mailto:pruegg@colobio.com] > Sent: Wednesday, 10 December 2003 9:12 AM > To: Histonet@Pathology. Swmed. Edu > Cc: Ihcrg@Yahoogroups. Com > Subject: [IHCRG] IHC on cells on coverslips > > Please advise how to manage IHC staining for cells grown on coverslips. > Patsy > > > > > > > ********************************************************************** > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message and any attachments are those > of the individual sender, and are not necessarily the views of The > Children's Hospital at Westmead > > This footnote also confirms that this email message has been > virus scanned and although no computer viruses were detected, > the Childrens Hospital at Westmead accepts no liability for any > consequential damage resulting from email containing computer > viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly <@t> merck.com Mon Dec 15 15:15:38 2003 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] For Colleen Forrester Message-ID: Colleen, Found it....it's in PBS. Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (Whitehouse Station, New Jersey, USA), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD) that may be confidential, proprietary copyrighted and/or legally privileged, and is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please immediately return this by e-mail and then delete it. ------------------------------------------------------------------------------ From weneng <@t> hotmail.com Mon Dec 15 16:24:18 2003 From: weneng <@t> hotmail.com (Wendy England) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] salary survey Message-ID: Thanks to everyone who sent me the 2002 salary survey web address! I am working in industry and want to know the salary range in industry in San Francisco bay area and east coast. Could anybody tell me or send me a linkage please? Many thanks! Wendy _________________________________________________________________ Winterize your home with tips from MSN House & Home. http://special.msn.com/home/warmhome.armx From lpwenk <@t> covad.net Mon Dec 15 18:37:55 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Gallyas Silver Technique References: <5.2.0.9.0.20031215135845.00a39860@mailhost.vetmed.auburn.edu> Message-ID: <003c01c3c36c$dd57a9e0$8732fea9@hppav> Here's a link: http://www.nottingham.ac.uk/pathology/protocols/gallyas.html >From Nottingham, England - lab of John Bancroft Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Atoska S. Gentry" To: "Histonet" Sent: Monday, December 15, 2003 3:00 PM Subject: [Histonet] Gallyas Silver Technique > > Hello, will someone familiar with Gallyas Silver Technique provide me with > a protocol and pointers? Thanks! Atoska > > > Atoska S. Gentry B.S., HT(ASCP) > Research Assistant III > Scott-Ritchey Research Center > College of Veterinary Medicine > Auburn University, AL 36849 > Phone# (334)844-5579 Fax# (334)844-5850 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SJones <@t> cvm.tamu.edu Mon Dec 15 19:55:09 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] off topic joke about management Message-ID: I think all of us working stiffs will appreciate this. Subject: And then there was a plan! Chapter 1, verses 1-15:1. In the beginning was the Plan. 2. And then came the Assumptions. 3. And the Assumptions were without form. 4. And the Plan was without Substance. 5. And darkness was upon the face of the Workers. 6. And the Workers spoke among themselves saying, "It is a crock of shit and it stinks." 7. And the Workers went unto their Supervisors and said, "It is a crock of dung and we cannot live with the smell." 8. And the Supervisors went unto their Managers saying, "It is a container of organic waste, and it is very strong, such that none may abide by it." 9. And the Managers went unto their Directors, saying, "It is a vessel of fertilizer, and none may abide its strength." 10. And the Directors spoke among themselves, saying to one another, "It contains that which aids plant growth, and it is very strong." 11. And the directors went to the Vice Presidents, saying unto them, "It promotes growth, and it is very powerful." 12. And the Vice Presidents went to the President, saying unto him, "It has very powerful effects." 13. And the President looked upon the Plan and saw that it was good. 14. And the Plan became Policy. 15. And that is how shit happens. Amen... Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From foerster <@t> CH11.UKL.UNI-FREIBURG.DE Tue Dec 16 05:25:17 2003 From: foerster <@t> CH11.UKL.UNI-FREIBURG.DE (Foerster, Katharina ) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Evans Blue and immunhistochemistry Message-ID: <3FDEF9AF.17081.F02DB9@localhost> Dear Histonetters, I`m looking for information about the parallel use of Evans Blue (to identify the area at risk induced by coronary artery ligation) and TTC (to identify the infarct zone) with a following immuno staining for vessels (anti-smooth-muscle-actin-antibody and cd31). Additionally, I wish to use Haematoxylin-Eosin staining and Elastica-van-Gieson in the heart-tissue. Evans Blue and TTC are necessary for macroscopic infarct size assessment and not replacable in this project. But it will be possible to take the tissue samples before TTC staining, so only Evans Blue (4 - 5 ml 10% dye per vena jugularis of the rabbit) remains problematical. I found some information about Evans Blue and H & E staining and this combination seems to be possible. But I`m not sure if Evans Blue interferes with or covers any of the other stainings. If so, is there a possibility to wash out Evans Blue before immunhistochemistry (heart tissue will be fixed in 4% formaldehyde before embedded in paraffin)? Or is the microscopic tissue discolouration negligible because of albumin-binding? Thank you for your help. Katrin. Katharina F?rster University Hospital Freiburg Dept. Heart and vessel surgery 79106 Freiburg Germany e-mail: foerster@ch11.ukl.uni-freiburg.de From Myri37 <@t> aol.com Tue Dec 16 07:06:08 2003 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] (no subject) Message-ID: <1CCDA28E.70493460.0005167B@aol.com> hello everyone does anyone know RBS stainning ? i heard that it's a very good stainning for resin sections... Thank you for anyhelp in advance Myriam baali natural Implant marseille France From simoskevitz <@t> osteotech.com Tue Dec 16 07:58:08 2003 From: simoskevitz <@t> osteotech.com (simoskevitz@osteotech.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] MMA Embedding Message-ID: I am trying to embed a polymer in MMA. The protocol I am using has me dehydrating the samples at 4C and then putting it into MMA for 1 week at -20C. The next week I am supposed to add BPO to the MMA and also keep it at -20C. When I looked at it the BPO is crystalizing out. Is this okay? Does anyone else embed at such a low temperature? We usually do it at 37C in a water bath. They want these particular samples done this way. Any advice for success would be greatly appreciated. Thank you, Ricki Simoskevitz Osteotech Inc. From fmonson <@t> wcupa.edu Tue Dec 16 09:38:30 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:21 2005 Subject: FW: [Histonet] Evans Blue and immunhistochemistry Message-ID: Morning Katrin, Evans Blue is known for its stochiometric reaction with albuminoids. Thus, it can be used for tests in the following manner. 1. One can inject the dye, in which case it binds with protein and nicely demonstrates extravasation of blood, etc. 2. One can go to the trouble of purchasing rabbit albumin or isolating it from rabbit blood (3.5+g/100ml) and reacting the Evans Blue in vitro before reintroducing it as an indicator. Because of its binding characteristics, other than a bleach (I have no knowledge of any such use), there is little hope to remove the dye. I prior work with rabbits and extravasation, we have also used Indigocarmine which does not bind to the tissues and readily leaches out of the tissue. Other help Why Certain Dyes Are Useful for Localizing the Sentinel Lymph Node Tsopelas and Sutton J Nucl Med.2002; 43: 1377-1382. http://jnm.snmjournals.org/cgi/reprint/44/9/1540.pdf Evans Blue is included in some Ab kits as a counterstain, thus, it would appear that its presence would not affect Ab binding. This, however, should be tested by you to demonstrate that EB has no effect on Ab reactions. http://www.researchd.com/viralab/proac1p.htm http://www.bindingsite.co.uk/antisera.asp http://cls.umc.edu/COURSES/cls411/serrxn2.doc Rasio, E., Hampers, C. L., Soeldner, J. S. and Cahill, G. F (1967). Diffusion of glucose, insulin, inulin and Evans-blue protein into thoracic duct lymph of man. J. Clin. Invest 46, 903-910. I searched Google with to find the above. Hope this helps and Merry Christmas, Frederick C. Monson, PhD Center for Advanced Scientific Imaging Mail to Geology West Chester University of Pennsylvania Schmucker II Science Center, Room SS024 South Church Street and Rosedale Avenue West Chester, PA, 19383 Phone/FAX: 610-738-0437 eMail: fmonson@wcupa.edu CASI Page and Scheduling http://darwin.wcupa.edu/CASI/ -----Original Message----- From: Foerster, Katharina [mailto:foerster@CH11.UKL.UNI-FREIBURG.DE] Sent: Tuesday, December 16, 2003 6:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Evans Blue and immunhistochemistry Dear Histonetters, I`m looking for information about the parallel use of Evans Blue (to identify the area at risk induced by coronary artery ligation) and TTC (to identify the infarct zone) with a following immuno staining for vessels (anti-smooth-muscle-actin-antibody and cd31). Additionally, I wish to use Haematoxylin-Eosin staining and Elastica-van-Gieson in the heart-tissue. Evans Blue and TTC are necessary for macroscopic infarct size assessment and not replacable in this project. But it will be possible to take the tissue samples before TTC staining, so only Evans Blue (4 - 5 ml 10% dye per vena jugularis of the rabbit) remains problematical. I found some information about Evans Blue and H & E staining and this combination seems to be possible. But I`m not sure if Evans Blue interferes with or covers any of the other stainings. If so, is there a possibility to wash out Evans Blue before immunhistochemistry (heart tissue will be fixed in 4% formaldehyde before embedded in paraffin)? Or is the microscopic tissue discolouration negligible because of albumin-binding? Thank you for your help. Katrin. Katharina F?rster University Hospital Freiburg Dept. Heart and vessel surgery 79106 Freiburg Germany e-mail: foerster@ch11.ukl.uni-freiburg.de _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RHESSLER <@t> mail.mcg.edu Tue Dec 16 09:55:36 2003 From: RHESSLER <@t> mail.mcg.edu (Richard Hessler) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds Message-ID: Skipped content of type multipart/alternative-------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:internet:rhessler@mail.mcg.edu TEL;WORK:9575 ORG:;Anatomic Pathology EMAIL;WORK;PREF:RHESSLER@mail.mcg.edu N:Hessler;Richard ADR;DOM;WORK;PARCEL;POSTAL:;BAE2571C LABEL;DOM;WORK;PARCEL;POSTAL;ENCODING=QUOTED-PRINTABLE:internet:rhessler@mail.mcg.edu=0A= BAE2571C X-GWUSERID:RHESSLER END:VCARD From mark.lewis <@t> thermo.com Tue Dec 16 10:34:05 2003 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds Message-ID: Richard, You will get more cracking of the paraffin blocks occurring when using the stainless steel base molds. Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com "Richard Hessler" To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] Plastic Embedding Molds western.edu 12/16/2003 10:55 AM I would like advise about disposable plastic embedding molds. Was wondering if any clinical labs had experienced problems with delays and uneven tissue alignment due to static and slow heat transfer. Also, what advantages do these have over traditional steel other than you don't clean them? As steel basically has an unlimited lifespan, are the disposable molds really cost effective in a clinical laboratory. Thanks Richard B Hessler, MD Chief, Section of Anatomic Pathology Associate Professor of Pathology and Neurology The Medical College of Georgia(See attached file: Richard Hessler.vcf) -------------- next part -------------- A non-text attachment was scrubbed... Name: Richard Hessler.vcf Type: application/octet-stream Size: 347 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/b001cfe4/RichardHessler.obj From kspencer <@t> utmem.edu Tue Dec 16 11:18:53 2003 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Divisions of hippocampus In-Reply-To: <20031213190304.92662.qmail@web20804.mail.yahoo.com> Message-ID: You need to buy a rat brain atlas. -Kathleen On Saturday, December 13, 2003, at 01:03 PM, David Laidley wrote: > Could anyone recommend a website, paper or text that clearly show the > divisions of the hippocampus in a rodent. Most specficially, I would > like to know where the boundries between CA1 and subiculum?exist in > coronal sections. > > > > > Post your free ad now! Yahoo! Canada Personals -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: text/enriched Size: 519 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/a894d60c/attachment.bin From mcauliff <@t> umdnj.edu Tue Dec 16 11:32:24 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Divisions of hippocampus In-Reply-To: References: Message-ID: <3FDF41A8.50201@umdnj.edu> There is a mouse brain atlas site at Harvard U, and at some other institutions as well. I will look it up tomorrow, class all afternoon today. Geoff Kathleen Spencer wrote: > You need to buy a rat brain atlas. > > -Kathleen > On Saturday, December 13, 2003, at 01:03 PM, David Laidley wrote: > > Could anyone recommend a website, paper or text that clearly show > the divisions of the hippocampus in a rodent. Most specficially, I > would like to know where the boundries between CA1 and > subiculum exist in coronal sections. > > > > > > Post your free ad now! Yahoo! Canada Personals > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/b119c10f/attachment.htm From mcauliff <@t> umdnj.edu Tue Dec 16 11:49:41 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Divisions of hippocampus In-Reply-To: References: Message-ID: <3FDF45B5.7010302@umdnj.edu> Try theses sites: www.mbl.org www.brainmuseum.org www.hms.harvard.edu/research/brain/methods.htm Or spring for "The Mouse brain in stereotaxic coordinates" by Paxinos and Franklin. Geoff Kathleen Spencer wrote: > You need to buy a rat brain atlas. > > -Kathleen > On Saturday, December 13, 2003, at 01:03 PM, David Laidley wrote: > > Could anyone recommend a website, paper or text that clearly show > the divisions of the hippocampus in a rodent. Most specficially, I > would like to know where the boundries between CA1 and > subiculum exist in coronal sections. > > > > > > Post your free ad now! Yahoo! Canada Personals > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/4bd044b5/attachment.htm From tpmorken <@t> labvision.com Tue Dec 16 11:59:22 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CE3B@usca0082k03.rallansci.apogent.com> Dr. Hessler wrote: "...what advantages do these have over traditional steel other than you don't clean them? As steel basically has an unlimited lifespan, are the disposable molds really cost effective in a clinical laboratory." Plastic molds: Disadvantages: Very high cost (although they can be reused for a while - just don't store in the heated space of the embedding center or they get warped) Hard to orient fragments due to some kind of electrostatic forces that move the pieces around. Slow to cool - again hard to work with small fragments and skin when you want to orient them a certain way. (though some may see as an advantage) Not as flat on the face as metal, so more trimming - bad for very small specimens. Do not hold heat the way metal does, so cools faster on the sides when time is spent orienting tissue. I see many more mis-oriented tissues in plastic molds, and mis-oriented mold/cassette problems (block face is mis-oriented to the cassette back, which means the block face is at an angle in the microtome chuck...= more trimming). Advantages: The sides of the mold are more vertical than the metal mold so a bit easier to cut (smaller face, sharper edge) Easy to take off the block (metal molds are often difficult to remove) Metal: Advantages Very low cost, last forever Flat face Cool fast Disadvantage Sometimes difficult to remove - use mold release (but that does take time and requires cleaning the molds daily in xylene/alcohol - we put them in the tissue processor and run the clean cycle) Cracked blocks? I haven't seen that as a problem. It is mainly in large blocks. You can put them on a piece of paper over the cooling plate to avoid that. It seems the only real advantage of the plastic is the shorter time spent taking the mold off the block and avoiding the time it takes to clean the metal ones (about 15 min/day max) If I was paying the bills I wouldn't get the plastic - they are far more expensive and do not give as good quality face as the metal. But...I haven't done a cost analysis of the plastic molds vs time spent to clean metal molds and then soak in mold release every day. Tim Morken -----Original Message----- From: Richard Hessler [mailto:RHESSLER@mail.mcg.edu] Sent: Tuesday, December 16, 2003 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic Embedding Molds I would like advise about disposable plastic embedding molds. Was wondering if any clinical labs had experienced problems with delays and uneven tissue alignment due to static and slow heat transfer. Also, what advantages do these have over traditional steel other than you don't clean them? As steel basically has an unlimited lifespan, are the disposable molds really cost effective in a clinical laboratory. Thanks Richard B Hessler, MD Chief, Section of Anatomic Pathology Associate Professor of Pathology and Neurology The Medical College of Georgia -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/781f7a16/attachment.htm From jqb7 <@t> cdc.gov Tue Dec 16 12:14:14 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds Message-ID: We use both in our lab and I prefer the metal molds for the reasons Tim listed. However, Tim mentioned one drawback of metal molds being the time required daily to clean them. Honestly, I find that cleaning and treating once a year is plenty, if that often. When I get new molds I spray them with any spray-on mold release (not the kind you have to soak the molds in) and that is usually sufficient. When I treat some older ones that have been previously used I run them through the clean cycle of the VIP (I know that is a no-no but I don't do it often!) and then apply the spray. I do not embed daily but when I was working at a small local hospital years ago where I did embed daily I found cleaning/spraying twice a year was sufficient. So I don't think the time involved in cleaning/handling metal molds is an issue at all. Just my point of view! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Morken, Tim - Labvision Sent: Tuesday, December 16, 2003 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic Embedding Molds Dr. Hessler wrote: "...what advantages do these have over traditional steel other than you don't clean them? As steel basically has an unlimited lifespan, are the disposable molds really cost effective in a clinical laboratory." Plastic molds: Disadvantages: Very high cost (although they can be reused for a while - just don't store in the heated space of the embedding center or they get warped) Hard to orient fragments due to some kind of electrostatic forces that move the pieces around. Slow to cool - again hard to work with small fragments and skin when you want to orient them a certain way. (though some may see as an advantage) Not as flat on the face as metal, so more trimming - bad for very small specimens. Do not hold heat the way metal does, so cools faster on the sides when time is spent orienting tissue. I see many more mis-oriented tissues in plastic molds, and mis-oriented mold/cassette problems (block face is mis-oriented to the cassette back, which means the block face is at an angle in the microtome chuck...= more trimming). Advantages: The sides of the mold are more vertical than the metal mold so a bit easier to cut (smaller face, sharper edge) Easy to take off the block (metal molds are often difficult to remove) Metal: Advantages Very low cost, last forever Flat face Cool fast Disadvantage Sometimes difficult to remove - use mold release (but that does take time and requires cleaning the molds daily in xylene/alcohol - we put them in the tissue processor and run the clean cycle) Cracked blocks? I haven't seen that as a problem. It is mainly in large blocks. You can put them on a piece of paper over the cooling plate to avoid that. It seems the only real advantage of the plastic is the shorter time spent taking the mold off the block and avoiding the time it takes to clean the metal ones (about 15 min/day max) If I was paying the bills I wouldn't get the plastic - they are far more expensive and do not give as good quality face as the metal. But...I haven't done a cost analysis of the plastic molds vs time spent to clean metal molds and then soak in mold release every day. Tim Morken -----Original Message----- From: Richard Hessler [mailto:RHESSLER@mail.mcg.edu] Sent: Tuesday, December 16, 2003 7:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic Embedding Molds I would like advise about disposable plastic embedding molds. Was wondering if any clinical labs had experienced problems with delays and uneven tissue alignment due to static and slow heat transfer. Also, what advantages do these have over traditional steel other than you don't clean them? As steel basically has an unlimited lifespan, are the disposable molds really cost effective in a clinical laboratory. Thanks Richard B Hessler, MD Chief, Section of Anatomic Pathology Associate Professor of Pathology and Neurology The Medical College of Georgia -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/d7064aa4/attachment.htm From ander093 <@t> gold.tc.umn.edu Tue Dec 16 12:25:24 2003 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds In-Reply-To: Message-ID: <5.2.0.9.0.20031216122416.00a1cc60@ander093.email.umn.edu> I agree with Jeanne. I too, prefer the metal molds. I reuse the molds until they start to become "sticky", then I clean, dry and spray with mold release. I don't deep track of specifically how often I clean them (maybe 2-3 times per year), but it is definitely not necessary to clean them after every use. I just put them back into the heat chamber and reuse. LuAnn Anderson HT(ASCP) Neuropathology Lab University of Minnesota At 01:14 PM 12/16/03 -0500, Bartlett, Jeanine wrote: >"urn:schemas-microsoft-com:office:office" xmlns:w = >"urn:schemas-microsoft-com:office:word" xmlns:st1 = >"urn:schemas-microsoft-com:office:smarttags"> >We use both in our lab and I prefer the metal molds for the reasons Tim >listed. However, Tim mentioned one drawback of metal molds being the time >required daily to clean them. Honestly, I find that cleaning and treating >once a year is plenty, if that often. When I get new molds I spray them >with any spray-on mold release (not the kind you have to soak the molds >in) and that is usually sufficient. When I treat some older ones that >have been previously used I run them through the clean cycle of the VIP (I >know that is a no-no but I don't do it often!) and then apply the spray. I >do not embed daily but when I was working at a small local hospital years >ago where I did embed daily I found cleaning/spraying twice a year was >sufficient. So I don't think the time involved in cleaning/handling metal >molds is an issue at all. > >Just my point of view! > >Jeanine Bartlett, HT(ASCP) >Centers for Disease Control >Infectious Disease Pathology Activity >1600 Clifton Road, MS/G-32 >Atlanta, GA 30333 >-----Original Message----- >From: histonet-admin@lists.utsouthwestern.edu >[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Morken, Tim >- Labvision >Sent: Tuesday, December 16, 2003 12:59 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Plastic Embedding Molds > >Dr. Hessler wrote: "...what advantages do these have over traditional >steel other than you don't clean them? As steel basically has an unlimited >lifespan, are the disposable molds really cost effective in a clinical >laboratory." > > > >Plastic molds: > >Disadvantages: > >Very high cost (although they can be reused for a while - just don't store >in the heated space of the embedding center or they get warped) > >Hard to orient fragments due to some kind of electrostatic forces that >move the pieces around. > >Slow to cool - again hard to work with small fragments and skin when you >want to orient them a certain way. (though some may see as an advantage) > >Not as flat on the face as metal, so more trimming - bad for very small >specimens. > >Do not hold heat the way metal does, so cools faster on the sides when >time is spent orienting tissue. I see many more mis-oriented tissues in >plastic molds, and mis-oriented mold/cassette problems (block face is >mis-oriented to the cassette back, which means the block face is at an >angle in the microtome chuck...= more trimming). > > > >Advantages: > >The sides of the mold are more vertical than the metal mold so a bit >easier to cut (smaller face, sharper edge) > >Easy to take off the block (metal molds are often difficult to remove) > > > > > >Metal: > >Advantages > >Very low cost, last forever > >Flat face > >Cool fast > > > >Disadvantage > >Sometimes difficult to remove - use mold release (but that does take time >and requires cleaning the molds daily in xylene/alcohol - we put them in >the tissue processor and run the clean cycle) > >Cracked blocks? I haven't seen that as a problem. It is mainly in large >blocks. You can put them on a piece of paper over the cooling plate to >avoid that. > > > >It seems the only real advantage of the plastic is the shorter time spent >taking the mold off the block and avoiding the time it takes to clean the >metal ones (about 15 min/day max) > >If I was paying the bills I wouldn't get the plastic - they are far more >expensive and do not give as good quality face as the metal. > >But...I haven't done a cost analysis of the plastic molds vs time spent to >clean metal molds and then soak in mold release every day. > > > > > >Tim Morken > > > >-----Original Message----- >From: Richard Hessler [mailto:RHESSLER@mail.mcg.edu] >Sent: Tuesday, December 16, 2003 7:56 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Plastic Embedding Molds > > > >I would like advise about disposable plastic embedding molds. Was >wondering if any clinical labs had experienced problems with delays and >uneven tissue alignment due to static and slow heat transfer. Also, what >advantages do these have over traditional steel other than you don't clean >them? As steel basically has an unlimited lifespan, are the disposable >molds really cost effective in a clinical laboratory. > > > >Thanks > > > >Richard B Hessler, MD >Chief, Section of Anatomic Pathology >Associate Professor of Pathology and Neurology >The Medical College of Georgia From jkiernan <@t> uwo.ca Tue Dec 16 12:31:15 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Divisions of hippocampus References: Message-ID: <3FDF4F73.F428593B@uwo.ca> kspencer@utmem.edu wrote: > Could anyone recommend a website, paper or text > that clearly show the divisions of the hippocampus > in a rodent. Most specficially, I would like > to know where the boundries between CA1 and > subiculum exist in coronal sections. Swanson LW 1998 Brain Maps: Structure of the Rat Brain. 2nd revised edn. Amsterdam: Elsevier. ISBN 0444827854 Very detailed labelled templates of coronal sections. There's also a CD with the book. For photos, the rat atlas by Paxinos is probably the best. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From jmitchell <@t> neurology.wisc.edu Tue Dec 16 12:25:29 2003 From: jmitchell <@t> neurology.wisc.edu (Mitchell (Jean A.)) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Bench top film/paper processor Message-ID: <061D5F3CF2DFDF4EADF5611CC1218C4512A645@nrl-lorenz.neurology.wisc.edu> I am searching for a distributor of either a bench top or small free standing darkroom film processor. All of my previous contacts are either defunct or no longer carry this product line. Any recommendations would be appreciated. Thanks! Jean Mitchell, BS, HT University of Wisconsin Hospital & Clinics Department of Neurology Madison, WI From Stephen.J.Scholz <@t> osfhealthcare.org Tue Dec 16 13:25:29 2003 From: Stephen.J.Scholz <@t> osfhealthcare.org (Scholz, Stephen J.) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] HTL exam Message-ID: <7F1312711CA7474A89B3DF8BA0BA54D0F5F0A5@pmc-rfd-mx01.intranet.osfnet.org> Hello Histonet, I am writing this for a fellow employee who would like to ask the Histonet a question. What text or workbooks should one get in order to study for the HTL exam? Thanks in advance, Steve -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/256aa6a7/attachment.htm From Rcartun <@t> harthosp.org Tue Dec 16 14:02:25 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Flow Cytometry Message-ID: Is anyone familiar with a laboratory that provides technical services only for flow cytometry (meaning that the referring pathologist will interpret the results)? Thank you. Richard Cartun From MinHan.Tan <@t> vai.org Tue Dec 16 14:28:35 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Sensitivity and specificity Message-ID: <74D0F0AB07F2E647A02D839ED79520F97B9C5F@VAIEXCH02.vai.org> Hi, We are having a little discussion here which we are unable to resolve to the satisfaction of one and all - we have found conflicting (as there are wont to be) information on the Internet. Is the ABC/DAB method more sensitive or more specific than immunofluorescence (direct/indirect)? Thank you very much! Regards, Min-Han Tan This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/7342cea4/attachment.htm From jefthompson <@t> salud.unm.edu Tue Dec 16 15:41:11 2003 From: jefthompson <@t> salud.unm.edu (Jeffrey Thompson) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Neurotacs II and stereology Message-ID: Hello Histonetters, I am wondering if anyone has tested the 'upper limit' of section thickness for TUNEL staining of neural tissue. I would like to perform stereology on my samples and to that end have relatively thick sections but I don't want to risk faulty counts due to poor labeling. I'd appreciate any input. Thanks, Jeff Thompson From Histolady710 <@t> aol.com Tue Dec 16 15:47:19 2003 From: Histolady710 <@t> aol.com (Histolady710@aol.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] HT Practical Message-ID: <1cd.161ba50d.2d10d767@aol.com> Has anyone received results from the HT Practical that was due Sept. 26, 2003 ? I think these were to be graded Nov. 5-6. Thanks ! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/7fff767d/attachment.htm From kbradshaw <@t> lcpath.com Tue Dec 16 17:19:48 2003 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] HT Practical In-Reply-To: <1cd.161ba50d.2d10d767@aol.com> Message-ID: We called ASCP, due to the high volume of slides to evaluate, results the not be out until the end of this month. Kari Bradshaw, HT(ASCP) Laboratory Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 (360)425-5620 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Histolady710@aol.com Sent: Tuesday, December 16, 2003 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT Practical Has anyone received results from the HT Practical that was due Sept. 26, 2003 ? I think these were to be graded Nov. 5-6. Thanks ! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031216/d506b39d/attachment.htm From ccdub <@t> earthlink.net Tue Dec 16 19:17:15 2003 From: ccdub <@t> earthlink.net (Cindy DuBois) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Labmaster Recycler Message-ID: We have a Labmaster Recycler. We have been trying to contact Chemical Managment Services (the place we get our supplies for our recycler from). They have gone out of business. Is anyone else still using a Labmaster and have a supplier? We need a new gasket and new fume scrubbers for it. Thank you, Cindy DuBois HT, ASCP DELTA PATHOLOGY ASSOCIATES STOCKTON CA From anissachoi <@t> hotmail.com Tue Dec 16 21:57:21 2003 From: anissachoi <@t> hotmail.com (Anissa Choi) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Re : Kappa and Lambda Message-ID: Does anyone pressure cook the paraffin sections before immunostaining for Kappa and Lambda? Our regular protocols : no pretreatment for B5, Protease for buffered formalin. I have found that there are a lot more plasma cells get stained after pressure cooking but backround is heavy though. Thanks in advance for your input. Anissa Choi Burnaby Hospital Burnaby, B.C. Canada _________________________________________________________________ STOP MORE SPAM with the new MSN 8 and get 2 months FREE* http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From John.Auld <@t> whnt.nhs.uk Wed Dec 17 04:39:47 2003 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Glacial acetic acid for Cervical CytologyThin Preps Message-ID: Dear all We have recently become aware of the use of Glacial acetic acid on Gynae specimens which appear inadequate after the normal protocol. We have used the GAA treatment on a few inadequates and found a marked improvement in the cellularity. Would anyone like to share their experiences with us. Many thanks John Auld Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral United Kingdom Tel 0151 604 7025 From TMcNemar <@t> lmhealth.org Wed Dec 17 05:02:42 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Re : Kappa and Lambda Message-ID: <90092A4ED388D7119575006008F7112049CB50@NT_EXCHANGE> We use Dako's antibodies with a steamer. They are beautiful. We no longer use the pressure cooker. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: Anissa Choi [mailto:anissachoi@hotmail.com] Sent: Tuesday, December 16, 2003 10:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re : Kappa and Lambda Does anyone pressure cook the paraffin sections before immunostaining for Kappa and Lambda? Our regular protocols : no pretreatment for B5, Protease for buffered formalin. I have found that there are a lot more plasma cells get stained after pressure cooking but backround is heavy though. Thanks in advance for your input. Anissa Choi Burnaby Hospital Burnaby, B.C. Canada _________________________________________________________________ STOP MORE SPAM with the new MSN 8 and get 2 months FREE* http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn .com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mark.lewis <@t> thermo.com Wed Dec 17 07:42:55 2003 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds Message-ID: Hello everyone, Do positively charged slides interfere when performing FISH ? I'm sure that it may depend on what the slides are positively charged with. I've not done any FISH so that's why I'm asking. Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com From gudrun.lang <@t> aon.at Wed Dec 17 08:16:37 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] gomori methenamin silver Message-ID: <002401c3c4a8$627a5500$eeeea8c0@SERVER> Dear histonetters, I need help of those, who are familiar to Gomori Methenamin Silver staining. I have troubles to convince my colleages about the advantages of using a stock meth-silver-solution. (instead of fresh making each time) stock: 1:40 10%Silvernitrate : 3% Hexamethenamin (stable for few months); working: 1:10 Borax : stock My colleages think, that the stock solution soon becomes instable. And that there is a kind of sediment at the bottom of the bottle (more concentration of silver). Can anyone tell me about bad experience with older stock solutions? What happens, when the stock solution is older than two (or three,..) months? Is the result of the stain weaker or darker? Question 2: What is the the function of Hexamethenamin? I know it causes the high pH, but does it make any compound with the silvernitrate? Any help is welcome Best wishes from Austria Gudrun Lang Akh Linz, Austria -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/10c6c28c/attachment.htm From jqb7 <@t> cdc.gov Wed Dec 17 08:32:06 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] gomori methenamin silver Message-ID: We use the Grocott's Method. Stock Solution A: 0.21% Silver nitrate (2.1 grams silver nitrate with 1000 ml distilled water): make in chemically clean glassware and refrigerate Stock Solution B: Methenamine-borax (27.0 grams methenamine, 3.8 grams borax, 1000 ml distilled water): make in chemically clean glassware and refrigerate Both stock solutions are stable for up to at least 4 months. We use equal portions of each for our working solution. We do not have a problem with sediment and the staining is just as good at 4 months as it is earlier. We haven't tried it beyond that time period because we always have it used up by then. I think the methenamine gives the silver the alkaline properties needed for a proper reaction. Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, December 17, 2003 9:17 AM To: Histonetliste Subject: [Histonet] gomori methenamin silver Dear histonetters, I need help of those, who are familiar to Gomori Methenamin Silver staining. I have troubles to convince my colleages about the advantages of using a stock meth-silver-solution. (instead of fresh making each time) stock: 1:40 10%Silvernitrate : 3% Hexamethenamin (stable for few months); working: 1:10 Borax : stock My colleages think, that the stock solution soon becomes instable. And that there is a kind of sediment at the bottom of the bottle (more concentration of silver). Can anyone tell me about bad experience with older stock solutions? What happens, when the stock solution is older than two (or three,..) months? Is the result of the stain weaker or darker? Question 2: What is the the function of Hexamethenamin? I know it causes the high pH, but does it make any compound with the silvernitrate? Any help is welcome Best wishes from Austria Gudrun Lang Akh Linz, Austria -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/0f2af817/attachment.htm From funderwood <@t> mcohio.org Wed Dec 17 08:51:39 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] gomori methenamin silver Message-ID: I do get a sediment that forms in the stock solution after several months. I have used it and still get good results. However, I have started replacing the stock solution after 6 if it hasn't been used up by then. Fred >>> "Gudrun Lang" 12/17/03 09:16AM >>> Dear histonetters, I need help of those, who are familiar to Gomori Methenamin Silver staining. I have troubles to convince my colleages about the advantages of using a stock meth-silver-solution. (instead of fresh making each time) stock: 1:40 10%Silvernitrate : 3% Hexamethenamin (stable for few months); working: 1:10 Borax : stock My colleages think, that the stock solution soon becomes instable. And that there is a kind of sediment at the bottom of the bottle (more concentration of silver). Can anyone tell me about bad experience with older stock solutions? What happens, when the stock solution is older than two (or three,..) months? Is the result of the stain weaker or darker? Question 2: What is the the function of Hexamethenamin? I know it causes the high pH, but does it make any compound with the silvernitrate? Any help is welcome Best wishes from Austria Gudrun Lang Akh Linz, Austria From JNocito <@t> Pathreflab.com Wed Dec 17 09:54:02 2003 From: JNocito <@t> Pathreflab.com (Joe Nocito) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Glacial acetic acid for Cervical CytologyThin Preps In-Reply-To: Message-ID: John, When we receive bloody (no pun intended) gyn Thinpreps, we treat them with G.A.A. because it lyses the RBC's, which causes the cellular material to really stand out. The cyto techs say there is an increased cellularity. We haven't tried this on conventional pap smears though. Joe Nocito, BS, HT (ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, Texas -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of John Auld Sent: Wednesday, December 17, 2003 4:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glacial acetic acid for Cervical CytologyThin Preps Dear all We have recently become aware of the use of Glacial acetic acid on Gynae specimens which appear inadequate after the normal protocol. We have used the GAA treatment on a few inadequates and found a marked improvement in the cellularity. Would anyone like to share their experiences with us. Many thanks John Auld Dept of Histopathology and Clinical Cytology Arrowe Park Hospital Arrowe Park Road Upton Wirral United Kingdom Tel 0151 604 7025 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Dec 17 10:10:39 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] steamers Message-ID: All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/673a92f5/attachment.htm From kmerriam2003 <@t> yahoo.com Wed Dec 17 11:04:17 2003 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] steamers In-Reply-To: Message-ID: <20031217170417.65350.qmail@web10002.mail.yahoo.com> I just use a Black & Decker Flavor Scenter Handy Steamer. About 30 bucks at Walmart! Kim Merriam Novartis Cambridge, MA "Bartlett, Jeanine" wrote: All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 --------------------------------- Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/16cb95ff/attachment.htm From simoskevitz <@t> osteotech.com Wed Dec 17 11:15:36 2003 From: simoskevitz <@t> osteotech.com (simoskevitz@osteotech.com) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Immuno stain for RBC's Message-ID: Does any one have a good protocol for an immuno stain for red blood vessels/endothelial cells on bone samples that have been embedded in glycol methacrylate? Thank you for any help you can give me. Ricki Simoskevitz Osteotech Inc. From rfail <@t> toolkitmail.com Wed Dec 17 10:33:16 2003 From: rfail <@t> toolkitmail.com (rfail) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] steamers Message-ID: <3fe0854c.1b0.1950.1668231422@toolkitmail.com> > Black and decker Rena All: > > Can anyone recommend a good, laboratory-grade steamer for > antigen retrieval? > > Thanks! > Jeanine Bartlett, HT(ASCP) > Centers for Disease Control > Infectious Disease Pathology Activity > 1600 Clifton Road, MS/G-32 > Atlanta, GA 30333 > From carole00 <@t> bellsouth.net Wed Dec 17 11:23:53 2003 From: carole00 <@t> bellsouth.net (Carole Cleveland) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] HCV testing Message-ID: <001e01c3c4c2$8b74e820$6400a8c0@VAIO> To: Richard Cartun, I know that Esoterix, Inc.- Molecular Genetics Lab is one of the only esoteric testing labs that performs HPV testing on paraffin embedded blocks; they may be able to do HCV testing as well. Here is their contact information: Esoterix Molecular Genetics - President - Ronald C. McGlennen, M.D. Hours of Operation: Monday - Friday, 8:00 a.m. - 5:00 p.m. CT Saturday, 8:00 a.m. - 1:00 p.m. CT 7550 Market Place Drive Eden Prairie, MN 55344 (866) 4GENETIX (1.866.443.6384) toll free (952) 941-7335 phone (952) 941-7249 fax Hope you find what you're looking for. Regards, Carole Cleveland carole00@bellsouth.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/06178c90/attachment.htm From jkiernan <@t> uwo.ca Wed Dec 17 12:01:32 2003 From: jkiernan <@t> uwo.ca (J. A. Kiernan) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] gomori methenamin silver References: <002401c3c4a8$627a5500$eeeea8c0@SERVER> Message-ID: <3FE099FC.DC8E6671@uwo.ca> > Gudrun Lang wrote: > Question 2: > What is the the function of Hexamethenamin? I know it causes > the high pH, but does it make any compound with the > silvernitrate? Methenamine = hexamine = hexamethylenetetramine. It is the soluble solid product of the rapid chemical reaction of formaldehyde with ammonia. It is used therapeutically, as tablets (Urasal) to treat urinary tract infections, and as an antiseptic and antiperspirant cream, Dehydral. Yes, methenamine does form a compound with silver. It is a complex comparable to the silver diammine ion that is formed when enough ammonia is added to a silver nitrate solution to dissolve the brown precipitate (silver oxide) that is first formed. The methenamine-silver complex is reduced to silver metal by reducing groups in the tissue - most notably by aldehyde groups that have been formed by oxidation (eg by dichromate, permanganate or periodate) in an earlier stage of the staining procedure. The methenamine-silver solution does the same job as Schiff's reagent. The black colour of the end product facilitates the detection very small or very thin objects such as bacteria and fungal hyphae. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ From MinHan.Tan <@t> vai.org Wed Dec 17 12:13:01 2003 From: MinHan.Tan <@t> vai.org (Tan, MinHan) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] steamers Message-ID: <74D0F0AB07F2E647A02D839ED79520F97B9C6D@VAIEXCH02.vai.org> Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends to run out of water if not topped up - it takes about 15-20 min to bring the temp of citrate buffer in a Coplin jar up to 95 deg C. Min-han Tan -----Original Message----- From: Kim Merriam [mailto:kmerriam2003@yahoo.com] Sent: Wednesday, December 17, 2003 12:04 PM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] steamers I just use a Black & Decker Flavor Scenter Handy Steamer. About 30 bucks at Walmart! Kim Merriam Novartis Cambridge, MA "Bartlett, Jeanine" wrote: All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _____ Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/939e37c5/attachment.htm From Bonnie.P.Whitaker <@t> uth.tmc.edu Wed Dec 17 12:33:23 2003 From: Bonnie.P.Whitaker <@t> uth.tmc.edu (Bonnie P Whitaker) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] steamers In-Reply-To: <74D0F0AB07F2E647A02D839ED79520F97B9C6D@VAIEXCH02.vai.org> Message-ID: MessageI use the B&D too, and I just fill the steamer fuller than you are supposed to..... almost to the top, and it always has enough for preheating and the longest time I use (40 min). The other thing I do sometimes (more for speed than for the reason you mention) is to heat the buffer in the microwave for a bit first. Then the preheating time is less than 20 minutes. Bonnie Whitaker University of Texas Medical School at Houston 6431 Fannin Street MSB 2.231 Houston, Texas 77030 Phone 713.500.6792 Fax 713.500.0733 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan Sent: Wednesday, December 17, 2003 12:13 PM To: Kim Merriam; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] steamers Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends to run out of water if not topped up - it takes about 15-20 min to bring the temp of citrate buffer in a Coplin jar up to 95 deg C. Min-han Tan -----Original Message----- From: Kim Merriam [mailto:kmerriam2003@yahoo.com] Sent: Wednesday, December 17, 2003 12:04 PM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] steamers I just use a Black & Decker Flavor Scenter Handy Steamer. About 30 bucks at Walmart! Kim Merriam Novartis Cambridge, MA "Bartlett, Jeanine" wrote: All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 ---------------------------------------------------------------------------- Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing ---------------------------------------------------------------------------- -- This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. ---------------------------------------------------------------------------- -- -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/71971684/attachment.htm From gudrun.lang <@t> aon.at Wed Dec 17 12:48:19 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] gomori methenamin silver References: <002401c3c4a8$627a5500$eeeea8c0@SERVER> <3FE099FC.DC8E6671@uwo.ca> Message-ID: <004301c3c4ce$573bcdb0$eeeea8c0@SERVER> Dear John, thank you, for the answer. The origin of my question is, that a fresh histotech in my lab made a mistake with the Grocott for Pneumocystis. Her working solution was 5% Silvernitrat (80ml) plus Natriumtetraborat (8ml), but without any Hexamin. The result was, that the doctor was really enthusiastic about the good quality. Today she tried it again. Untill now I don't know the result. Do you think, Hexamin is really necessary for the proper reaction? And if yes, what has happend? Gudrun Lang ----- Original Message ----- From: "J. A. Kiernan" To: "Gudrun Lang" Cc: "Histonetliste" Sent: Wednesday, December 17, 2003 7:01 PM Subject: Re: [Histonet] gomori methenamin silver > > Gudrun Lang wrote: > > > Question 2: > > What is the the function of Hexamethenamin? I know it causes > > the high pH, but does it make any compound with the > > silvernitrate? > > Methenamine = hexamine = hexamethylenetetramine. > It is the soluble solid product of the rapid > chemical reaction of formaldehyde with ammonia. > It is used therapeutically, as tablets (Urasal) to > treat urinary tract infections, and as an antiseptic > and antiperspirant cream, Dehydral. > > Yes, methenamine does form a compound with > silver. It is a complex comparable to the silver > diammine ion that is formed when enough ammonia > is added to a silver nitrate solution to dissolve > the brown precipitate (silver oxide) that is > first formed. The methenamine-silver complex is > reduced to silver metal by reducing groups in > the tissue - most notably by aldehyde groups that > have been formed by oxidation (eg by dichromate, > permanganate or periodate) in an earlier stage of > the staining procedure. The methenamine-silver > solution does the same job as Schiff's reagent. > The black colour of the end product facilitates > the detection very small or very thin objects > such as bacteria and fungal hyphae. > -- > ------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan@uwo.ca > http://publish.uwo.ca/~jkiernan/ > > From kspencer <@t> utmem.edu Wed Dec 17 12:59:31 2003 From: kspencer <@t> utmem.edu (Kathleen Spencer) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] Plastic Embedding Molds In-Reply-To: Message-ID: <255E2DF8-30C3-11D8-85A3-000393967904@utmem.edu> I use Fisher brand charged slides for FISH. Works great. -Kathleen On Wednesday, December 17, 2003, at 07:42 AM, mark.lewis@thermo.com wrote: > > Hello everyone, > > Do positively charged slides interfere when performing FISH ? > > I'm sure that it may depend on what the slides are positively charged > with. > > I've not done any FISH so that's why I'm asking. > > > > Best regards, > > Mark > > Mark Lewis > Product Specialist > Anatomical Pathology > Clinical Diagnostics > Thermo Electron Corporation > (412) 747-4013 > (412) 788-1097 > E-mail: mark.lewis@thermo.com > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> colobio.com Wed Dec 17 13:31:45 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:21 2005 Subject: [Histonet] steamers In-Reply-To: <74D0F0AB07F2E647A02D839ED79520F97B9C6D@VAIEXCH02.vai.org> Message-ID: I use the steamer and heat the ar buffer in the mw to boiling before putting it in the steamer that way it only takes about 5 min. to preheat. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan Sent: Wednesday, December 17, 2003 11:13 AM To: Kim Merriam; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] steamers Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends to run out of water if not topped up - it takes about 15-20 min to bring the temp of citrate buffer in a Coplin jar up to 95 deg C. Min-han Tan -----Original Message----- From: Kim Merriam [mailto:kmerriam2003@yahoo.com] Sent: Wednesday, December 17, 2003 12:04 PM To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] steamers I just use a Black & Decker Flavor Scenter Handy Steamer. About 30 bucks at Walmart! Kim Merriam Novartis Cambridge, MA "Bartlett, Jeanine" wrote: All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 _____ Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing _____ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you. _____ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/b928ee1c/attachment.htm From jean-ross <@t> uiowa.edu Wed Dec 17 13:27:29 2003 From: jean-ross <@t> uiowa.edu (jean-ross@uiowa.edu) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] automatic slide stainer Message-ID: <1071689249.3fe0ae21c3027@webmail1.its.uiowa.edu> Hello everyone, We are looking at getting an automatic slide stainer in the lab and a used Sakura DRS-601 has become available. I am interested in hearing comments from any of you who have used this particular stainer. Is it reliable? Easy to use? How many slides can you stain per run? Good or bad experiences? Any comments would be welcome. Thanks in advance. Jean Ross Research Assistant Central Microscopy Research Facility University of Iowa From dmccaig <@t> ckha.on.ca Wed Dec 17 14:02:14 2003 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Fouchets for Bile Message-ID: <3E5A3F039F0BD8118B4700C00D002024042FFC@CKHA9> We are having difficulties getting a positive reaction for this stain. Can anyone provide me with a stain method, choice of controls and any special advice regarding this stain? Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) From subratab <@t> bdonline.com Wed Dec 17 14:38:29 2003 From: subratab <@t> bdonline.com (Subratab) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] bovine albumin and BSA; if replacable Message-ID: <200312172042.hBHKgCZ6004366@korotoa.bdonline.com> Dear All Please tell me if albumin, bovine (Sigma A-2153) can be used instead of bovine serum albumin (BSA) (Sigma B-4287) as diluent of antibody for IHC? What are the differences of the uses of albumin, bovine and BSA; and why ? Thanks in advance Subrata Biswas Dept of Nephrology UNICAMP, SP, Brazil From pruegg <@t> colobio.com Wed Dec 17 15:00:18 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Immuno stain for RBC's In-Reply-To: Message-ID: It can be difficult to do IHC on GMA embedded samples especially if extreme care has not been taken during fixation and processing and embedding (not to overheat). Plus since you cannot remove the plastic you must pass thru it and the IHC reagents contain such large molecules it can be difficult to get them thru the plastic. I know some people do IHC on GMA but in my experience it is very difficult and inconsistent. The recommended media for IHC would be methyl methacrylate which can be removed from the section before IHC staining. Good antibodies for vessel lining cells include cd31, vegf, desmin, factor VIII, cd34, thrombomudulin, and agglutinin. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of simoskevitz@osteotech.com Sent: Wednesday, December 17, 2003 10:16 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Immuno stain for RBC's Does any one have a good protocol for an immuno stain for red blood vessels/endothelial cells on bone samples that have been embedded in glycol methacrylate? Thank you for any help you can give me. Ricki Simoskevitz Osteotech Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From huffpw <@t> uleth.ca Wed Dec 17 15:02:53 2003 From: huffpw <@t> uleth.ca (Phillip Huff) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] bovine albumin and BSA; if replacable In-Reply-To: <200312172042.hBHKgCZ6004366@korotoa.bdonline.com> References: <200312172042.hBHKgCZ6004366@korotoa.bdonline.com> Message-ID: <1842.142.66.42.15.1071694973.squirrel@webmail.uleth.ca> Both of these products are an enriched form of bovine serum albumin. A-2153 is the most commonly used and, as far as I remember, the cheaper of the two. The lower cost is simply due to a lower degree of purity (96%). We use A-2153 for our enzyme assays as a carrier of acyl-CoA, and I have used it for IHC without any problems. B-4287 is also albumin from a bovine serum source but has been purified to the extreme. As noted below, it is recommended for Southerns (the blot, not people from the South ;)) The information below is from Sigma's website. I would recommend A-2153 because (i) its cheaper, and (ii) should suit your needs. As far as I am aware, BSA is commonly used for a number of biochemical applications because the source, bovine serum, is readily available in most beef eating countries. In addition, the work done on purifying these proteins was optimized by people investigating cattle, where they obtained their samples from slaughter houses. Take a look at the work done by Christian Anfinsen (ca. 1950's Boston) and his work determining the disulfide bonding nature of ribonuclease. He got all his samples from the slaughterhouse next door. A-2153 Albumin bovine serum (pH 7 minimum 96% (electrophoresis) Lyophilized powder) - Although it may be used as a carrier for hapten immunization in animals other than goats and sheep, it is more commonly used as a carrier for hapten in immunoassays. The small size of BSA, its abundance in serum, and the similarity between BSA and albumins from other species makes it less desirable for antibody production. B-4287 Albumin bovine serum (for molecular biology, powder) - A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications. Phil Dear All > Please tell me if albumin, bovine (Sigma A-2153) can be used instead of > bovine serum albumin (BSA) (Sigma B-4287) as diluent of antibody for IHC? > What are the differences of the uses of albumin, bovine and BSA; and why ? > > Thanks in advance > Subrata Biswas > Dept of Nephrology > UNICAMP, SP, Brazil > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mark.lewis <@t> thermo.com Wed Dec 17 15:35:04 2003 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Body Lift for Morgue/Autopsy Room Message-ID: Marsha Price asked Can anyone tell me where a good source is to buy a body lift. I have Mopecs' Catalog. Just wondering if there were any other companies that sell Morgue supplies. Yes, Thermo Electron ( formerly Thermo Shandon, Shandon, and Shandon Lipshaw ) sells morgue and autopsy supplies Call 1-800-245-6212 ext. 4013 Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com mprice26@juno.com Sent by: To: histonet@lists.utsouthwestern.edu histonet-admin@lists.utsouth cc: western.edu Subject: [Histonet] Body Lift for Morgue/Autopsy Room 12/11/2003 11:14 AM Histonetters, Can anyone tell me where a good source is to buy a body lift. I have Mopecs' Catalog. Just wondering if there were any other companies that sell Morgue supplies. Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Wed Dec 17 16:10:38 2003 From: jstaruk <@t> masshistology.com (Mass Histology Service) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Body Lift for Morgue/Autopsy Room In-Reply-To: Message-ID: There are lots of funeral home suppliers who carry that sort of equipment. Working in pathology, you must know some funeral directors who can help you out. Happy holidays everyone! Jim ____________________ James Staruk, HT(ASCP) Mass Histology Service A division of DVMLab, inc. www.masshistology.com -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of mark.lewis@thermo.com Sent: Wednesday, December 17, 2003 4:35 PM To: histonet@pathology.swmed.edu Subject: Re: [Histonet] Body Lift for Morgue/Autopsy Room Marsha Price asked Can anyone tell me where a good source is to buy a body lift. I have Mopecs' Catalog. Just wondering if there were any other companies that sell Morgue supplies. Yes, Thermo Electron ( formerly Thermo Shandon, Shandon, and Shandon Lipshaw ) sells morgue and autopsy supplies Call 1-800-245-6212 ext. 4013 Best regards, Mark Mark Lewis Product Specialist Anatomical Pathology Clinical Diagnostics Thermo Electron Corporation (412) 747-4013 (412) 788-1097 E-mail: mark.lewis@thermo.com mprice26@juno.com Sent by: To: histonet@lists.utsouthwestern.edu histonet-admin@lists.utsouth cc: western.edu Subject: [Histonet] Body Lift for Morgue/Autopsy Room 12/11/2003 11:14 AM Histonetters, Can anyone tell me where a good source is to buy a body lift. I have Mopecs' Catalog. Just wondering if there were any other companies that sell Morgue supplies. Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Dec 17 16:45:33 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] gomori methenamin silver References: <002401c3c4a8$627a5500$eeeea8c0@SERVER> <3FE099FC.DC8E6671@uwo.ca> <004301c3c4ce$573bcdb0$eeeea8c0@SERVER> Message-ID: <3FE0DC8D.FFD092AC@uwo.ca> If you mix the silver nitrate and sodium tetraborate as you describe, there will be a white precipitate of silver borate. It is possible to make a borate-buffered very dilute silver nitrate solution, as in the Holmes method (for axons in paraffin sections of CNS). The mechanism of that technique is quite different from that of Grocott's or any other methenamine (hexamine) silver method. If it stains Pneumocystis that's fine, but the mechanism will have to be different. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ ------------------------- Gudrun Lang wrote: > > Dear John, > thank you, for the answer. > > The origin of my question is, that a fresh histotech in my lab made a > mistake with the Grocott for Pneumocystis. Her working solution was 5% > Silvernitrat (80ml) plus Natriumtetraborat (8ml), but without any Hexamin. > The result was, that the doctor was really enthusiastic about the good > quality. > Today she tried it again. Untill now I don't know the result. > > Do you think, Hexamin is really necessary for the proper reaction? And if > yes, what has happend? > > Gudrun Lang > > ----- Original Message ----- > From: "J. A. Kiernan" > To: "Gudrun Lang" > Cc: "Histonetliste" > Sent: Wednesday, December 17, 2003 7:01 PM > Subject: Re: [Histonet] gomori methenamin silver > > > > Gudrun Lang wrote: > > > > > Question 2: > > > What is the the function of Hexamethenamin? I know it causes > > > the high pH, but does it make any compound with the > > > silvernitrate? > > > > Methenamine = hexamine = hexamethylenetetramine. > > It is the soluble solid product of the rapid > > chemical reaction of formaldehyde with ammonia. > > It is used therapeutically, as tablets (Urasal) to > > treat urinary tract infections, and as an antiseptic > > and antiperspirant cream, Dehydral. > > > > Yes, methenamine does form a compound with > > silver. It is a complex comparable to the silver > > diammine ion that is formed when enough ammonia > > is added to a silver nitrate solution to dissolve > > the brown precipitate (silver oxide) that is > > first formed. The methenamine-silver complex is > > reduced to silver metal by reducing groups in > > the tissue - most notably by aldehyde groups that > > have been formed by oxidation (eg by dichromate, > > permanganate or periodate) in an earlier stage of > > the staining procedure. The methenamine-silver > > solution does the same job as Schiff's reagent. > > The black colour of the end product facilitates > > the detection very small or very thin objects > > such as bacteria and fungal hyphae. > > -- > > ------------------------- > > John A. Kiernan > > Department of Anatomy and Cell Biology > > The University of Western Ontario > > London, Canada N6A 5C1 > > kiernan@uwo.ca > > http://publish.uwo.ca/~jkiernan/ > > > > From ploykasek <@t> phenopath.com Wed Dec 17 18:44:15 2003 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] steamers In-Reply-To: Message-ID: > I agree with Bonnie about preheating the HIER buffer ? quick & easy. Also, we > prefer the oval shaped steamer over the round one. The oval one holds more > water. We do fill it up to the brim! > > Patti Loykasek > PhenoPath Laboratories > Seattle, WA > > > I use the B&D too, and I just fill the steamer fuller than you are supposed > to..... almost to the top, and it always has enough for preheating and the > longest time I use (40 min). The other thing I do sometimes (more for speed > than for the reason you mention) is to heat the buffer in the microwave for a > bit first. Then the preheating time is less than 20 minutes. > Bonnie Whitaker > University of Texas Medical School at Houston > 6431 Fannin Street > MSB 2.231 > Houston, Texas 77030 > Phone 713.500.6792 > Fax 713.500.0733 >> >> -----Original Message----- >> From: histonet-admin@lists.utsouthwestern.edu >> [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tan, MinHan >> Sent: Wednesday, December 17, 2003 12:13 PM >> To: Kim Merriam; Bartlett, Jeanine; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] steamers >> >> Yes, we use that too. Only problem is that for long ARs (30-35 min), it tends >> to run out of water if not topped up - it takes about 15-20 min to bring the >> temp of citrate buffer in a Coplin jar up to 95 deg C. >> >> Min-han Tan >> >> >>> -----Original Message----- >>> From: Kim Merriam [mailto:kmerriam2003@yahoo.com] >>> Sent: Wednesday, December 17, 2003 12:04 PM >>> To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu >>> Subject: Re: [Histonet] steamers >>> >>> I just use a Black & Decker Flavor Scenter Handy Steamer. About 30 bucks at >>> Walmart! >>> >>> Kim Merriam >>> Novartis >>> Cambridge, MA >>> >>> "Bartlett, Jeanine" wrote: >>>> All: >>>> >>>> Can anyone recommend a good, laboratory-grade steamer for antigen >>>> retrieval? >>>> >>>> Thanks! >>>> Jeanine Bartlett, HT(ASCP) >>>> Centers for Disease Control >>>> Infectious Disease Pathology Activity >>>> 1600 Clifton Road, MS/G-32 >>>> Atlanta, GA 30333 >>> >>> Do you Yahoo!? >>> New Yahoo! Photos - easier uploading and sharing >>> >> com> >> >> This email message, including any attachments, is for the sole use of the >> intended recipient(s) and may contain confidential information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If you >> are not the intended recipient(s) please contact the sender by reply email >> and destroy all copies of the original message. Thank you. > > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031217/af9c53a9/attachment.htm From L.D.Ridley <@t> bham.ac.uk Thu Dec 18 03:24:36 2003 From: L.D.Ridley <@t> bham.ac.uk (LD Ridley) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Steamer Protocol hints Message-ID: <1071739476.c0ab7e00L.D.Ridley@bham.ac.uk> Hi all, Does anyone out there have any good tips they could share with me with regards to using steamers for antigen retrieval. We have just had one kindly donated. My plan is to place a container of cold citrate buffer with the slides in, into the steamer and heat for approximately 40 mins. (Allowing the temp of the citrate buffer to reach ~95 degress C after around 20 mins) Does anyone think a hot start would be preferential. i.e heat the citrate buffer first before adding the slides. Also what kind of retrieval solutions do people use, we make our retrieval solution up ourselves but does anybody use commercially availible ones. I know i'm going to have have a 'tinker' with this protocol to get optimal results.. Thanks in advance for your help Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 From Hedley.Glencross <@t> CMMC.nhs.uk Thu Dec 18 03:32:03 2003 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] senisitivity & specificity Message-ID: Hi Tan In cytology we use measures of sensitivity amnd specificity to monitor the screening programme. A sensitive test is one that identifies abnormal results and a specific test is one that identifies normal results. As to whether ICC is more sensitive or more specific than IF, I'm not sure. I know when I did histology many years ago, we stuck with IF as it was more sensitive due to the background with ICC, but that was a long time ago and methodology has changed in that time. My guess now would be that it may be very much dependent how well the technique is performed, but then is that not always the case! Merry Xmas Hedley Glencross Manchester Cytology Centre UK Message: 8 Date: Tue, 16 Dec 2003 15:28:35 -0500 From: "Tan, MinHan" To: Subject: [Histonet] Sensitivity and specificity This is a multi-part message in MIME format. ------_=_NextPart_001_01C3C413.2E01732E Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable Hi, =20 We are having a little discussion here which we are unable to resolve to the satisfaction of one and all - we have found conflicting (as there are wont to be) information on the Internet. =20 Is the ABC/DAB method more sensitive or more specific than immunofluorescence (direct/indirect)? Thank you very much! =20 Regards, Min-Han Tan From lpwenk <@t> covad.net Thu Dec 18 04:04:55 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] HTL exam References: <7F1312711CA7474A89B3DF8BA0BA54D0F5F0A5@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <008601c3c54e$6312c000$8732fea9@hppav> HTL examThe following is a link to the ASCP Board of Registry web page, listing the reading lists. Note: you need Adobe 5.0 or higher to read the page. http://www.ascp.org/bor/certification/reading/ This list is also sent to everyone who applies. No, you don't have to order all the books. My own personal minimum recommendation for HTL (for what it's worth: - any good atlas for tissue I - start with the Carson books to get the basics - then study the Bancroft/Gamble book for more details and unusual stains - get as many NSH Self-Assessment booklets to practice taking exams. (Suggestion to supervisors - buy these booklets for the lab. Use them to train new people, and use them to assess the cognitive (knowledge) competency of your histotechs (a CAP and JCAHO requirement).) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: Scholz, Stephen J. To: histonet@pathology.swmed.edu Sent: Tuesday, December 16, 2003 2:25 PM Subject: [Histonet] HTL exam Hello Histonet, I am writing this for a fellow employee who would like to ask the Histonet a question. What text or workbooks should one get in order to study for the HTL exam? Thanks in advance, Steve -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/43a6bfa0/attachment.htm From lpwenk <@t> covad.net Thu Dec 18 04:10:27 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Fouchets for Bile References: <3E5A3F039F0BD8118B4700C00D002024042FFC@CKHA9> Message-ID: <008d01c3c54f$291a8d00$8732fea9@hppav> Try this link http://www.nottingham.ac.uk/pathology/protocols/fouchet.html We make the trichloroacetic acid solution up fresh each time. Control would be a liver with bile plugs. Check some cirrhotic livers for these. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: "Diana McCaig" To: Sent: Wednesday, December 17, 2003 3:02 PM Subject: [Histonet] Fouchets for Bile > We are having difficulties getting a positive reaction for this stain. Can > anyone provide me with a stain method, choice of controls and any special > advice regarding this stain? > Diana McCaig, R.T. > Charge Tech, Histology > Chatham Kent Health Alliance > 519-352-6401 (3347) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From M.Bromley <@t> dgri.scot.nhs.uk Thu Dec 18 04:14:46 2003 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] paddle forceps Message-ID: <7325D637DFE2D211928800902733A7F303B949FA@DGAMTBDCEMS> Hi All I am having great difficulty getting a pair of paddle forceps, the flat mesh type, ideal for thin slicing breast. The type I am after (AB042) was on the Mopec website only a few weeks ago but has now disappeared, I have e-mailed Mopec without success. Surgipath and Medim in the UK were unable to help. Does anyone have an alternative? I would much appreciate any help. Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/078cd8c4/attachment.htm From RFail <@t> Charleston.net Thu Dec 18 04:33:27 2003 From: RFail <@t> Charleston.net (Rena Fail) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Steamer Protocol hints In-Reply-To: <1071739476.c0ab7e00L.D.Ridley@bham.ac.uk> Message-ID: <000801c3c552$6083daf0$b311a6a5@rena> I preheat the buffer while the slides are dehydrating The temp is usually at 99 at the end of 20 minutes. Fisher sells a digital thermometer with a probe that fits thru the holes in the lid of the steamer and placed in the coplin jar We use the retrieval solutions from DAKO Rena Fail -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of LD Ridley Sent: Thursday, December 18, 2003 4:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Steamer Protocol hints Hi all, Does anyone out there have any good tips they could share with me with regards to using steamers for antigen retrieval. We have just had one kindly donated. My plan is to place a container of cold citrate buffer with the slides in, into the steamer and heat for approximately 40 mins. (Allowing the temp of the citrate buffer to reach ~95 degress C after around 20 mins) Does anyone think a hot start would be preferential. i.e heat the citrate buffer first before adding the slides. Also what kind of retrieval solutions do people use, we make our retrieval solution up ourselves but does anybody use commercially availible ones. I know i'm going to have have a 'tinker' with this protocol to get optimal results.. Thanks in advance for your help Lee Lee Ridley Research Associate L.D.Ridley@bham.ac.uk Cancer Research UK Institute for Cancer Studies University of Birmingham Vincent Drive Edgbaston Birmingham B15 2TT UK +44 (0)121 414 4472 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Thu Dec 18 07:47:52 2003 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Body Lift for Morgue/Autopsy Room Message-ID: Triangle Scientific sells Jewett morgue equipment, which has lifts, and is very heavy duty . Tim Harris is the rep. in our area. They will send you a catalog. Who may email at www.tharris@trianglescientific.com. Their phone number is 1-800-846-8081 ex.12. Good luck, Carole Fields Lex.Med.Ctn. W.Columbia, SC > -----Original Message----- > From: mark.lewis@thermo.com [SMTP:mark.lewis@thermo.com] > Sent: Wednesday, December 17, 2003 4:35 PM > To: histonet@pathology.swmed.edu > Subject: Re: [Histonet] Body Lift for Morgue/Autopsy Room > > Marsha Price asked > Can anyone tell me where a good source is to buy a body lift. I have > Mopecs' Catalog. Just wondering if there were any other companies that > sell > Morgue supplies. > > > Yes, > > Thermo Electron ( formerly Thermo Shandon, Shandon, and Shandon Lipshaw ) > sells morgue and autopsy supplies > Call 1-800-245-6212 ext. 4013 > > Best regards, > > Mark > > Mark Lewis > Product Specialist > Anatomical Pathology > Clinical Diagnostics > Thermo Electron Corporation > (412) 747-4013 > (412) 788-1097 > E-mail: mark.lewis@thermo.com > > > > > > mprice26@juno.com > > Sent by: To: > histonet@lists.utsouthwestern.edu > histonet-admin@lists.utsouth cc: > > western.edu Subject: > [Histonet] Body Lift for Morgue/Autopsy Room > > > > > 12/11/2003 11:14 AM > > > > > > > > > > > Histonetters, > Can anyone tell me where a good source is to buy a body lift. I have > Mopecs' Catalog. Just wondering if there were any other companies that > sell > Morgue supplies. > > Thank you. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/da0afc28/attachment.htm From cfavara <@t> niaid.nih.gov Thu Dec 18 09:12:24 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] paddle forceps Message-ID: Try Fine Science Tools w ww.finescience.com c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Mike Bromley [mailto:M.Bromley@dgri.scot.nhs.uk] Sent: Thursday, December 18, 2003 3:15 AM To: Histonet (E-mail) Subject: [Histonet] paddle forceps Hi All I am having great difficulty getting a pair of paddle forceps, the flat mesh type, ideal for thin slicing breast. The type I am after (AB042) was on the Mopec website only a few weeks ago but has now disappeared, I have e-mailed Mopec without success. Surgipath and Medim in the UK were unable to help. Does anyone have an alternative? I would much appreciate any help. Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK This e-mail and any files transmitted with it are private and intended solely for the use of the individual or entity to whom they are addressed. If you have received this e-mail in error please return it to the address it came from telling them it is not for you and then delete it from your system. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/d498574d/attachment.htm From clarkda <@t> ohsu.edu Thu Dec 18 10:17:26 2003 From: clarkda <@t> ohsu.edu (David Clark) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Cryostat Message-ID: Hello, Can anyone recommend a good/great cryostat for Mohs. I'm looking for one that has lots of room to work in. I do not need a lot of bells and whistles just one that is comfortable to operate. If you have a reliable source for a used one that would be great also. Many thanks for any and all replies. Happy Holidays! David OHSU/Kaiser Portland, Oregon From juan.gutierrez <@t> christushealth.org Thu Dec 18 10:45:58 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Looking for CD4,CD8,EGFR.Collagen IV antibodies Message-ID: I'm not sure about the Nexes, but we use Ventana's CD4 and CD8 on our Benchmarks with good results. For Collagen IV we use a prep-kit and our primary is from DAKO cat.#M0785, clone CIV 22. We no longer offer EGFR. Hope this helps you. Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Nava, Josefa [mailto:JosefaNava@texashealth.org] Sent: Fri 12/12/2003 1:24 PM To: 'histonet@pathology.swmed.edu' Cc: Subject: [Histonet] Looking for CD4,CD8,EGFR.Collagen IV antibodies Hello Everyone, Can you tell me where I can find the following antibodies, CD4,CD8, Collagen IV and EGFR and what clone is the best , that will work with Ventana Nexes. I thank you so much. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Thu Dec 18 10:58:21 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Cryostat Message-ID: If you want lots of room, try the big Microm cryostats. I specially like the HM560 with the vacutome. Good luck on your search. Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: David Clark [mailto:clarkda@ohsu.edu] Sent: Thu 12/18/2003 10:17 AM To: Histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Cryostat Hello, Can anyone recommend a good/great cryostat for Mohs. I'm looking for one that has lots of room to work in. I do not need a lot of bells and whistles just one that is comfortable to operate. If you have a reliable source for a used one that would be great also. Many thanks for any and all replies. Happy Holidays! David OHSU/Kaiser Portland, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Dec 18 12:52:23 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Zeus Fixative Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C7E@EXCHANGE1.huntingtonhospital.com> Can someone tell me where I can order Zeus Fixative? Are there any large distributors that carry this? Laurie Colbert Huntington Hospital Pasadena CA From cgfields <@t> lexhealth.org Thu Dec 18 13:06:43 2003 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Zeus Message-ID: Zeus fixative...Carter-Wallace Wampole, P.O. Box 1001 Half Ave. Road, Cranberry, N.J. 085102. 1-800-257-9525 Zeus Fixative 0102 12/pkg $55.15 Zeus Wash Sol. 0103 120 ml. $37.62 Carole Fields Lex. Med. Ctn W. Columbia, SC _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/d9759a17/attachment.htm From subratab <@t> bdonline.com Thu Dec 18 13:45:52 2003 From: subratab <@t> bdonline.com (Subratab) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] bovine albumin and BSA; if replacable Message-ID: <200312181949.hBIJnk7l023827@korotoa.bdonline.com> An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/aa84e37c/attachment.htm From david.kinsley <@t> spcorp.com Thu Dec 18 15:32:41 2003 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: unsubscribe David Kinsley Schering-Plough Research Institute Kenilworth, NJ 07033 908-740-4669 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/d3aed0b4/attachment.htm From CTague <@t> ahs.llumc.edu Thu Dec 18 15:40:32 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] vvg ? Message-ID: do varicose veins work for elastic controls? Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From AnthonyH <@t> chw.edu.au Thu Dec 18 15:48:40 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] vvg ? Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E112@simba.kids> Curtis, I would recommend skin (especially a child or young adult) as an elastic control. The subdermis is rich in both course and fine fibres and allows good differentiation to be achieved. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: Tague, Curtis [mailto:CTague@ahs.llumc.edu] Sent: Friday, 19 December 2003 8:41 AM To: Histonet (E-mail) Subject: [Histonet] vvg ? do varicose veins work for elastic controls? Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From bills <@t> icpmr.wsahs.nsw.gov.au Thu Dec 18 15:57:20 2003 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Cryostat In-Reply-To: Message-ID: <000301c3c5b1$e9c80930$3187080a@wsahs.nsw.gov.au> David, We have a Shandon E660 which has the microtome outside the freezing chamber, it has just the head in the chamber leaving plenty of room around the chamber. All the best Bill Sinai Laboratory Manager Tissue Pathology, ICPMR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of David Clark Sent: Friday, 19 December 2003 3:17 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello, Can anyone recommend a good/great cryostat for Mohs. I'm looking for one that has lots of room to work in. I do not need a lot of bells and whistles just one that is comfortable to operate. If you have a reliable source for a used one that would be great also. Many thanks for any and all replies. Happy Holidays! David OHSU/Kaiser Portland, Oregon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From carole00 <@t> bellsouth.net Thu Dec 18 22:37:11 2003 From: carole00 <@t> bellsouth.net (Carole Cleveland) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Flow Cytometry Technical Only Services Message-ID: <001901c3c5e9$c68c9660$6600a8c0@CCleveland> Richard, In response to your Flow Cytometry question, I believe that US Labs provides a "Technical Only" testing component for interpretation of results by your own Pathologist. I had also heard that Esoterix Oncology in Brentwood, TN may provide this service as well, but that is unconfirmed. Carole Cleveland Cell: 321-223-8918 Fax: 954-385-8213 e-mail: carole00@bellsouth.net From: "Richard Cartun" -------------------------------------------------------------------------------- Is anyone familiar with a laboratory that provides technical services only for flow cytometry (meaning that the referring pathologist will interpret the results? -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/bb415596/attachment.htm From carole00 <@t> bellsouth.net Thu Dec 18 22:37:43 2003 From: carole00 <@t> bellsouth.net (Carole Cleveland) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Flow Cytometry Technical Only Services Message-ID: <001d01c3c5e9$d7fd9d40$6600a8c0@CCleveland> Richard, In response to your Flow Cytometry question, I believe that US Labs provides a "Technical Only" testing component for interpretation of results by your own Pathologist. I had also heard that Esoterix Oncology in Brentwood, TN may provide this service as well, but that is unconfirmed. Carole Cleveland e-mail: carole00@bellsouth.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031218/006303f7/attachment.htm From ames1 <@t> breathe.com Fri Dec 19 04:36:50 2003 From: ames1 <@t> breathe.com (ames1@breathe.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Acridine Orange Staining Message-ID: I have been trying out Acridine Orange to identify Micronuclei in Rat bone marrow. Unfortunately it hasn't worked. I was wondering if anyone has any experience with this stain and could suggest a workable method. Also any advice on how to view the slides i.e wavelength, block type (FITC, Texas red etc). My thanks to anyone who can help Amy Greenhalgh From am102 <@t> st-andrews.ac.uk Fri Dec 19 05:08:20 2003 From: am102 <@t> st-andrews.ac.uk (Anne-Sophie Martinez) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] proteins in vesicles staining Message-ID: <200312191059.KAA29929@bute.st-andrews.ac.uk> Hello everybody!!! I want to stain in IHC a protein (guanylin) which is supposed to be in intra-cellular vesicles in the epithelium. Could somebody let me know (1) if there is a protocol (and which one) to premeabilize these vesicles to allow the AB to fix on the protein inside? or (2) if something better does exist? Thank you very much. Merry Christmas. Anne-Sophie ---------------------------------------------- Dr Anne-Sophie Martinez School of Biology University of St. Andrews Bute Medical Buildings St. Andrews Fife, KY16 9TS UK. Tel + 44 (0) 1334 463546 Fax + 44 (0) 1334 463600 From Michelle.Rederick <@t> bayfront.org Fri Dec 19 09:24:55 2003 From: Michelle.Rederick <@t> bayfront.org (Rederick, Michelle) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Histotech position opening Message-ID: Hi histonetters! We have an opening for a histotechnician/histotechnologist at Bayfront Medical Center in beautiful St. Petersburg, FL. This is a full time day shift position working for the nicest group of pathologists around! Send me an e-mail if you would like more info. P.S. A common misconception among histotechs is that you need to take an exam to receive FL technician/technologist licensure...but you don't. You just have to provide proof of education, proof of ASCP registration, and pay a small fee. So please do not let this deter you from applying in Florida. Thanks!! Michelle Rederick HTL(ASCP)"This transmission may contain confidential health information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after it's stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and arrange for the return or destruction of these documents." From Ngilman <@t> nbhd.org Fri Dec 19 09:41:14 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Job Opportunity Message-ID: We will have a histotechnologist position opening January 1, 2004 here at Broward General Medical Center in beautiful and warm (just not today) Ft. Lauderdale, Florida. This is a full time, Monday-Friday position Working hours are 7AM-3:30 P, but we can be flexible if needed. We do lots of special stains and Immunoperoxidase procedures and will be getting into CISH in the very near future. If you or someone you know is interested, contact me at the following numbers. Thanks Noreen Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager **************************************** NORTH BROWARD HOSPITAL DISTRICT CONFIDENTIALITY NOTICE: This message and any included attachments are intended for the sole use of the individual or entity to which it is addressed. This message may contain information that is confidential and protected by federal and state law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply e-mail and then delete the original message and its attachments without reading or saving the attachments in any manner. Thank you. **************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/30b11523/attachment.htm From Michelle.Rederick <@t> bayfront.org Fri Dec 19 09:41:18 2003 From: Michelle.Rederick <@t> bayfront.org (Rederick, Michelle) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Pituitary Control Block Message-ID: Hi histonetters! I am in need of a pituitary control block. We are having a hard time obtaining this tissue and I need a good control for IHC. Any help would be greatly apptreciated. Many Thanks, Michelle Rederick HTL(ASCP) Bayfront Medical Center St. Petersburg, FL"This transmission may contain confidential health information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after it's stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and arrange for the return or destruction of these documents." From cgfields <@t> lexhealth.org Fri Dec 19 09:55:37 2003 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Job Opportunity Message-ID: Ditto: We have an opening for a histotechnician/histotechnologist at Lexington Medical Center in W.Columbia, SC. Short drive from Florida, Charleston, Hilton Head, Savannah, and Myrtle Beach. This is a full time day shift position working for a very nice group of pathologists. Send me an e-mail if you would like more info. You can go on line at www.lexmed.com and fill out an application and e-mail it to me at www.cgfields@lexhealth.org.... do not email it to Human Resources it seems to get lost. If you have a resume you may attach it also. We will be interviewing after the holidays. Thank you Carole Fields Lex. Med. Ctn. W.Columbia, SC _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/cb5f7d89/attachment.htm From david.kinsley <@t> spcorp.com Fri Dec 19 09:56:20 2003 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: unsubscribe David Kinsley Schering-Plough Research Institute Kenilworth, NJ 07033 908-740-4669 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/439ce52e/attachment.htm From Samuel.Jones2 <@t> med.va.gov Fri Dec 19 10:55:21 2003 From: Samuel.Jones2 <@t> med.va.gov (Jones, Samuel) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Vacancy-Dallas, Texas Message-ID: The VA Medical Center, Dallas, is seeking a certified Histology Technician/Technologist (ASCP) to fill a full time position within the Histology Section of the Anatomic Pathology Department. The successful applicant must have the ability to produce quality work in routine histology and special stains. The VAMC-Dallas is a full-service facility in the heart of the VA North Texas Health Care System . We have recently moved into a new $155 million clinical addition that includes state-of-the-art instrumentation and technology. As part of our team, you will have the opportunity to work with quality, advanced technology and equipment. Interested applicants should address question to Patrick Kunke, Chief Medical Technologist, Monday through Friday, at 214-857-0682. The VAMC-Dallas is an EEO Employer. Samuel E. Jones, MS, HT (ASCP) HTL, QIHC Supervisor Anatomic Pathology VA North Texas Healthcare System 4500 South Lancaster Road 113 Dallas, Texas 75216 Phone: 214-857-0659 Fax: 214-302-1457 E-mail: samuel.jones2@med.va.gov From siksik03 <@t> comcast.net Fri Dec 19 10:52:08 2003 From: siksik03 <@t> comcast.net (Steven E. Slap) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] steamers In-Reply-To: References: Message-ID: Jeanine & HistoNetters There is no such thing as a "laboratory-grade" steamer, if what you mean is a steamer made especially for use in laboratories. I'm sure that you will get recommendations from people of steamers that have been used successfully in laboratories, but this is by no means the same thing. best regards, Steven Slap >All: > >Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? > >Thanks! >Jeanine Bartlett, HT(ASCP) >Centers for Disease Control >Infectious Disease Pathology Activity >1600 Clifton Road, MS/G-32 >Atlanta, GA 30333 > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/3622f10f/attachment.htm From jqb7 <@t> cdc.gov Fri Dec 19 10:58:39 2003 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] steamers Message-ID: Thanks. I was thinking along the same lines as a laboratory microwave versus a home microwave. Definitely different than what you buy at Best Buy. We have a household steamer we use here, I was just hoping there was something a little better out there. -----Original Message----- From: Steven E. Slap [mailto:siksik03@comcast.net] Sent: Friday, December 19, 2003 11:52 AM To: Bartlett, Jeanine; HistoNet Server Subject: Re: [Histonet] steamers Jeanine & HistoNetters There is no such thing as a "laboratory-grade" steamer, if what you mean is a steamer made especially for use in laboratories. I'm sure that you will get recommendations from people of steamers that have been used successfully in laboratories, but this is by no means the same thing. best regards, Steven Slap All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/5ab54440/attachment.htm From i_stain <@t> yahoo.com Fri Dec 19 11:32:06 2003 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Looking for CD4,CD8,EGFR.Collagen IV antibodies Message-ID: <20031219173206.81845.qmail@web42006.mail.yahoo.com> Hi josie, We use CD4 (1F6, 1:40, HIER with EDTA), CD8 (1A5, HIER with EDTA) & EGFR (31G7, 1:40, Protease 1) from Zymed. We are not doing collagen IV, but Zymed also sell them. You can contact them to get more info. Scott -----Original Message----- From: Nava, Josefa [mailto:JosefaNava@texashealth.org] Sent: Fri 12/12/2003 1:24 PM To: 'histonet@pathology.swmed.edu' Cc: Subject: [Histonet] Looking for CD4,CD8,EGFR.Collagen IV antibodies Hello Everyone, Can you tell me where I can find the following antibodies, CD4,CD8, Collagen IV and EGFR and what clone is the best , that will work with Ventana Nexes. I thank you so much. Josie __________________________________ Do you Yahoo!? New Yahoo! Photos - easier uploading and sharing. http://photos.yahoo.com/ From georgecole <@t> ev1.net Fri Dec 19 12:33:18 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Negative response from the Journal of Neuropathology and Experimental Neurology Message-ID: <000001c3c65e$93548080$094dbad0@hppav> Histotechs; Most of you have noticed my offer of muscle and nerve packets offered AT NO CHARGE to all interested histotechs. It has been showing up on the Histonet since July Well, I was hoping to add similar offers to neuropathologists by way of the Journal of Neuropathology and Experimental Neurology. I got a most negative response from the Editor in Chief of that journal, Michael Noel Hart, who, apparently without reading the letter I sent or the packet material, said "I think you just want free advertisement for your product" I hit the ceiling and wrote a reply from there, but I haven't come in for a landing yet. Histotechs, you have given a warm reception to those packets---I've sent you 87 packets so far to 33 states and 14 countries outside the US at NO CHARGE. It seemed logical to include neuropathologists in the offer, because decisions were made in my lab by my very great boss, Dr. Anthony D'Agostino, neuropathologist, and I assumed things were much the same in all your labs. The packet was, again, offered AT NO CHARGE to the good doctors. May I assume that the neuropathologists in your lab have heard about the better methods. If it would do any good, I would be happy to send you histotechs copies, as needed, for the neuropathologists involved in your muscle and nerve procedures. They will surely be involved in any decisions about USING the methods contained in the packets. And folks, NOT using the methods can only lead to spurious No Pathological Diagnoses in some percentage of your muscle and nerve cases. This is not a matter of taste---it's a matter of necessity----searching, thoroughly, those tissues sent for histochemistry, which is NOT done by the "pip-of-muscle-on-a-cork" method in almost universal use. I know folks take some getting used to free offers---it seems almost weird, I guess to Dr. Hart---no pumping folks for their cash---so he just jumped at conclusions and landed on me. Well, I became addicted to the greater effort to search those tissues----I actually spent 2.5 years worth of unpaid overtime at Good Sam Hospital here, and left behind 39 weeks of unused vacation time in developing and doing those procedures contained in the packets. I hardly believe I actually did that----but I did----I was thinking of getting fitted for a plastic electric halo at the time, but my head started swelling every time I was fitted, so I just winged it, bare headed. You know my computer's correction function keeps changing histonet to hemstitch----well whenever I started writing anyone about my goody goody doings, that corrector kept changing the phrase "noble efforts" to the "dojngs of a jerk!!". Is there anybody out there who knows how to turn the Truth off on this danged HP keyboard? Anyway, if there is one or more neuropathologists involved in deciding what methods to use in your muscle and nerve lab, I will be very happy to send any packets needed for them to see. Incidently, a product of my green, green computer skills----when I made the first video DVD, I figured, as it would hold 80 minutes of sound, it might hold 40 minutes of sight AND sound> A sure enough., there was just a little clear band at the center of the CD, showing, I thought, that what I put on it just barely fit. So there I went sending all of you three DVD's to contain the video material. Well, somehow, I fouled up, recently, making a set of DVD';s to send and the whole video landed on ONE CD! I looked at it, and the circle in the center of the CD was about 2/3's if the way to the edge. The circle I had seen in the center of the CD was the RECORDED area. Now that just leaves leventy seven billion things I don't know about computers! So, if any of you have tromped upon any of the CD's I sent you with your spiky boots, I will send you one CD with the whole shebang on one CD. I know that pure thought transfer may be coming up, replacing keyboards and common computers ---but I don't really think I'll get one----I don't want anybody reading that stuff!! . georgecole@ev1,net computer? -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/edfda517/attachment.htm From DDittus787 <@t> aol.com Fri Dec 19 12:51:13 2003 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Negative response from the Journal of Neuropathology and Experimental Neurology Message-ID: <1296FAB4.22B453C5.0A1F969F@aol.com> george: i have received your most generous procedures and i am disheartened to hear that a scientific journal was so closed to free assistance, however take heart in knowing "no good deed goes unpunished", and i too at times due to offers have faced skepticism, prejudice and maybe even some petty jealousy, but please know i truly appreciate your work and kindness and remember: people are often unreasonable,illogical and self centered-forgive them if you are kind people may accuse you of ulterior motives-be kind anyway if you are successful you will win some false friends-and some true enemies-succeed anyway if you are honest people may cheat you-be honest anyway give the world the best you have and it may never be enough- give your best anyway......and thats what you are trying to do george so don't let them stop you. Dana From histobabs <@t> hotmail.com Fri Dec 19 13:09:25 2003 From: histobabs <@t> hotmail.com (Barbara Terrett) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] fibronectin Message-ID: Is anyone out there using fibronectin on ffpe tissues, and if so, what company are you getting it from and are you happy with it. The pathologist requesting it here is leaning towards the Dako monoclonal......is anyone using that? Thanks, Barb _________________________________________________________________ The new MSN 8: advanced junk mail protection and 2 months FREE* http://join.msn.com/?page=dept/bcomm&pgmarket=en-ca&RU=http%3a%2f%2fjoin.msn.com%2f%3fpage%3dmisc%2fspecialoffers%26pgmarket%3den-ca From EliMarGo <@t> aol.com Fri Dec 19 14:44:29 2003 From: EliMarGo <@t> aol.com (EliMarGo@aol.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] sectioning Message-ID: <0F118582.557E817B.0071FAF8@aol.com> Hello everyone, This is the first time I use histonet and I hope I do it right.:) I have a pretty general question and hope you can help me with it. Can anyone tell me what is the average number of slides one tech. can section per an 8 hour day? I was recently asked this question by my boss and truthfully I can produce 250+ slides for serial cuts, more if it is regular sectioning. The reason I am asking is because I am curious to know if this is the norm or if I have to work on my speed. I also have to add that I have only been doing this type of work for about 1.5 years now.Hope someone can give me the information I seek. Happy Holidays everyone!! Sincerely, Elisa Gonzalez Research Tech. II Doheny Eye Institute Pathology Department From pam <@t> ategra.com Fri Dec 19 14:31:35 2003 From: pam <@t> ategra.com (Pam Barker (extension 234)) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Seasons Greetings from Pam Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/1f4284b1/attachment.htm -------------- next part -------------- A non-text attachment was scrubbed... Name: seasons greetings 4.jpg Type: image/jpeg Size: 18423 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/1f4284b1/seasonsgreetings4.jpg From pruegg <@t> colobio.com Fri Dec 19 15:29:58 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Negative response from the Journal of Neuropathology and Experimental Neurology In-Reply-To: <000001c3c65e$93548080$094dbad0@hppav> Message-ID: George, I appreciate all your work and encourage you to submit your work for publication in the Journal of Histotechnology, I will see that you are offered all the assistance you may need. Sincerely, Patsy Ruegg Assistant Editor, JOH -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of George Cole Sent: Friday, December 19, 2003 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative response from the Journal of Neuropathology and Experimental Neurology Histotechs; Most of you have noticed my offer of muscle and nerve packets offered AT NO CHARGE to all interested histotechs. It has been showing up on the Histonet since July Well, I was hoping to add similar offers to neuropathologists by way of the Journal of Neuropathology and Experimental Neurology. I got a most negative response from the Editor in Chief of that journal, Michael Noel Hart, who, apparently without reading the letter I sent or the packet material, said ?I think you just want free advertisement for your product? I hit the ceiling and wrote a reply from there, but I haven?t come in for a landing yet. Histotechs, you have given a warm reception to those packets---I?ve sent you 87 packets so far to 33 states and 14 countries outside the US at NO CHARGE. It seemed logical to include neuropathologists in the offer, because decisions were made in my lab by my very great boss, Dr. Anthony D?Agostino, neuropathologist, and I assumed things were much the same in all your labs. The packet was, again, offered AT NO CHARGE to the good doctors. May I assume that the neuropathologists in your lab have heard about the better methods. If it would do any good, I would be happy to send you histotechs copies, as needed, for the neuropathologists involved in your muscle and nerve procedures. They will surely be involved in any decisions about USING the methods contained in the packets. And folks, NOT using the methods can only lead to spurious No Pathological Diagnoses in some percentage of your muscle and nerve cases. This is not a matter of taste---it?s a matter of necessity----searching, thoroughly, those tissues sent for histochemistry, which is NOT done by the ?pip-of-muscle-on-a-cork? method in almost universal use. I know folks take some getting used to free offers---it seems almost weird, I guess to Dr. Hart---no pumping folks for their cash---so he just jumped at conclusions and landed on me. Well, I became addicted to the greater effort to search those tissues----I actually spent 2.5 years worth of unpaid overtime at Good Sam Hospital here, and left behind 39 weeks of unused vacation time in developing and doing those procedures contained in the packets. I hardly believe I actually did that----but I did----I was thinking of getting fitted for a plastic electric halo at the time, but my head started swelling every time I was fitted, so I just winged it, bare headed. You know my computer?s correction function keeps changing histonet to hemstitch----well whenever I started writing anyone about my goody goody doings, that corrector kept changing the phrase ?noble efforts? to the ?dojngs of a jerk!!?. Is there anybody out there who knows how to turn the Truth off on this danged HP keyboard? Anyway, if there is one or more neuropathologists involved in deciding what methods to use in your muscle and nerve lab, I will be very happy to send any packets needed for them to see. Incidently, a product of my green, green computer skills----when I made the first video DVD, I figured, as it would hold 80 minutes of sound, it might hold 40 minutes of sight AND sound> A sure enough., there was just a little clear band at the center of the CD, showing, I thought, that what I put on it just barely fit. So there I went sending all of you three DVD?s to contain the video material. Well, somehow, I fouled up, recently, making a set of DVD?;s to send and the whole video landed on ONE CD! I looked at it, and the circle in the center of the CD was about 2/3?s if the way to the edge. The circle I had seen in the center of the CD was the RECORDED area. Now that just leaves leventy seven billion things I don?t know about computers! So, if any of you have tromped upon any of the CD?s I sent you with your spiky boots, I will send you one CD with the whole shebang on one CD. I know that pure thought transfer may be coming up, replacing keyboards and common computers ---but I don?t really think I?ll get one----I don?t want anybody reading that stuff!! . georgecole@ev1,net computer? -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/faec1fd2/attachment.htm From pruegg <@t> colobio.com Fri Dec 19 15:32:00 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] steamers In-Reply-To: Message-ID: I am sure a laboratory grade steamer is on the mind of some vendor, it may go the way of MW and pressure cooker. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Steven E. Slap Sent: Friday, December 19, 2003 9:52 AM To: Bartlett, Jeanine; HistoNet Server Subject: Re: [Histonet] steamers Jeanine & HistoNetters There is no such thing as a "laboratory-grade" steamer, if what you mean is a steamer made especially for use in laboratories. I'm sure that you will get recommendations from people of steamers that have been used successfully in laboratories, but this is by no means the same thing. best regards, Steven Slap All: Can anyone recommend a good, laboratory-grade steamer for antigen retrieval? Thanks! Jeanine Bartlett, HT(ASCP) Centers for Disease Control Infectious Disease Pathology Activity 1600 Clifton Road, MS/G-32 Atlanta, GA 30333 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/eafd84c1/attachment.htm From pruegg <@t> colobio.com Fri Dec 19 15:37:48 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] vvg ? In-Reply-To: Message-ID: Curtis, I do a lot of elastic fiber work on arteries, not sure the vein's would be so good. Lung tissue can show a lot of arteries with elastic fibers right next to veins that do not have the fibers so much. Patsy -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Tague, Curtis Sent: Thursday, December 18, 2003 2:41 PM To: Histonet (E-mail) Subject: [Histonet] vvg ? do varicose veins work for elastic controls? Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patowck <@t> nus.edu.sg Fri Dec 19 20:12:05 2003 From: patowck <@t> nus.edu.sg (Ow Cheok Kee) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: This email is confidential and may be privileged. If you are not the intended recipient, please delete it and notify us immediately; you should not copy or use it for any purpose, nor disclose its contents to any other person. Thank you." _____ Upgrade Your Email - Click here! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031220/69eaba2a/attachment.htm From georgecole <@t> ev1.net Fri Dec 19 20:26:11 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] PAS PROCEDURE FROM MUSCLE/NERVE PACKET Message-ID: <000001c3c6a0$a312a140$0a4dbad0@hppav> For Those Who Received Muscle & Nerve packets; Sorry, when I made up the packets, I used forms filled out for our last inspection by office staff. The typing was so much better than my own. All the ingredients were listed, but the actual PAS procedure was left out. The following procedure is done after the PAS reagents are prepared: This follows page 12: 1. Put slides in the fixative 10 minutes to one hour. 2. Rinse well in deionized water. 3. Place in freshly mixed 0.5% aqueous Periodic Acid 5 minutes. 4. Wash thoroughly in deionized water. 5. Place in Schiff's Reagent 15 minutes. 6. Rinse in three changes of acid rinse for two minutes each. 7. Wash in gently running water for a minute or two. Rinse with deionized water. Stain with Gill's III hematoxylin for 30 seconds to a minute. Blue and rinse with running tap water 15 minutes. Rinse in deionized water. 8 . Dehydrate with 95% and 100% alcohols. Clear in xylene and mount in Permount or equivalent. DIASTASE DIGESTION PROCEDURE: 1. Fix for 10 minutes. 2. Rinse well. 3. Digest in filtered Diastase solution for one hour at 37C. 4. Rinse well in deionized water. 5 Proceed with regular PAS routine from step 3 above. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031219/ec96b8d4/attachment.htm From tissuearray <@t> hotmail.com Sat Dec 20 00:49:29 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] sectioning Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031220/7696752f/attachment.htm From Linresearch <@t> aol.com Sat Dec 20 07:41:39 2003 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] VW Factor Message-ID: <33.42334acd.2d15ab93@aol.com> Hello, I need to work up FFPE & ZFPE canine tissues for VW Ab and VWPP(propeptide). If anyone with a protocol or any helpful hints is willing to share their information, I would appreciate it. Thanks, lin -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031220/31bf0229/attachment.htm From gudrun.lang <@t> aon.at Sat Dec 20 13:18:54 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] sectioning References: <0F118582.557E817B.0071FAF8@aol.com> Message-ID: <003801c3c72e$1bb781a0$eeeea8c0@SERVER> We use only slide microtomes for routine histology. I am working as a histotech for ten years. My average speed is 10 slides in 10-15 min (one cut on one slide); and 10 slides in 20-25 min (4 cuts on one slide, biopsies) We (2-3 persons) cut every day from 7 to 11. And each person does ca. 80-100 slides to manage the extent of one day. After this we are happy to stand up. I cannot believe, that anyone cuts eight hours a day without any troubles. (on body and mind) Gudrun Lang Linz, Austria ----- Original Message ----- From: To: Sent: Friday, December 19, 2003 9:44 PM Subject: [Histonet] sectioning > Hello everyone, > > This is the first time I use histonet and I hope I do it right.:) I have a pretty general question and hope you can help me with it. Can anyone tell me what is the average number of slides one tech. can section per an 8 hour day? > > I was recently asked this question by my boss and truthfully I can produce 250+ slides for serial cuts, more if it is regular sectioning. The reason I am asking is because I am curious to know if this is the norm or if I have to work on my speed. I also have to add that I have only been doing this type of work for about 1.5 years now.Hope someone can give me the information I seek. > > Happy Holidays everyone!! > > > Sincerely, > > Elisa Gonzalez > Research Tech. II > Doheny Eye Institute > Pathology Department > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histology.bc <@t> shaw.ca Sat Dec 20 23:03:46 2003 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Sectioning Message-ID: <3FE529B2.8070309@shaw.ca> I know you have had a couple of responses to your question already, but I thought I should throw my thoughts in too. Personally, I would hate to sit and just cut for eight houirs straight. Unless you have an ergonomically-designed workstation and chair, you are asking for all kinds of stress and strains to various body parts ... to say nothing about wrist and shoulder problems. Take breaks, divide your cutting into shorther sessions, or get someone else to help out. The actual number of sections cut at the end of an eight hour shift is one of those potentially meaningless statistics ... the number is dependent on so many variables: - Are you talking about one sections per block (ie 250 sections from 250 different blocks) or 250 sections from the same block? - Are the blocks already trimmed? - What kind of tissue is being sectioned? - How thick are the sections? - How many sections per slide? - Are your slides prelabelled? - Do you pick up your own sections? If the blocks are pre-trimmed, pre-chilled, and you are cutting one section per slide, I would consider an average of 2 blocks/slides per minute to be reasonable. Any additional work (trimming, loading up the cold tray, etc) will slow you down. If you are cutting multiple sections from blocks, a higher number of slides should be expected as the amount of trimming, securing blocks in the chuck, etc, is reduced. However, speed is not the critical factor. Quality is. High quality sections are essential. Poor quality sections will prevent the microscopist from seeing relevant detail. I taught histology for over 20 years, and one of my standard sermons about section cutting (and many other things) was "Get good first ... then get fast !!!" Don't be too concerned about your speed, ask your "boss" if he is happy with the quality of your sections. This is the critical question. Don't make a deliberate effort to try to cut fast, speed will come ... with practice. Rushing will only reduce quality and put your fingers at risk. Paul From gudrun.lang <@t> aon.at Sun Dec 21 03:21:25 2003 From: gudrun.lang <@t> aon.at (Gudrun Lang) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] sectioning References: <000001c3c73c$a5d15330$0b4dbad0@hppav> Message-ID: <001001c3c7a3$cec612f0$eeeea8c0@SERVER> George, In Austria and Germany it is common to use sliding microtomes. In our lab we use rotary microtoms only for making serial cuts of renal biopsies. Trimming and sectioning is really faster using sliding microtomes. Perhaps it demands more effort, because of moving the slide forwards and backwards fast, but with the modern microtomes it became much more easier. We cut tissue from smallest lung biopsies, bone marrow-biopsies to all routine surgical tissues. We are able to cut at 1 to 3 and more micrometer thickness. Trimming: 5-10 x with 30 um; then 2-3x with 3 um to get an even surface. Then take off a good cut with 3 um, put it on the water bath with a brush, mounting it on the slide and ready. You get allways single cuts and no ribbons. I have found a link, where you can look at a modern sliding microtom. Ours look a little big different. http://www.microm.de/microm%20homepage/microm_deutsch/html/hm_450_d.html I hope my English was good enough to explain and I would be glad if you visit our small city. Gudrun Lang Linz, Austria ----- Original Message ----- From: "George Cole" To: "'Gudrun Lang'" Sent: Saturday, December 20, 2003 10:02 PM Subject: RE: [Histonet] sectioning > Gudrun; > The sliding microtome you mention---the only sliding microtomes I came > across in my long career, were used for extremely difficult sectioning. > They were a bit awkward to use. Section thickness was always pretty > large. Would a rotary microtome, which seems to be standard in the labs > I've seen, be easier and faster to use? I never did regular daily > surgicals. I was always cutting muscles and nerves, with some kidneys > in the days when I did immunofluorescnce studies of them. I also cut > occasional liver, diaphragm, and brain tissues, but never on an all day > routine. It sounds like you work up a pretty good speed on the > mnicrotome you use, whatever it is. Nice to hear from you---I would like > to visit Linz and other Mozart territories. > georgecole@ev1.net > > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Saturday, December 20, 2003 11:19 AM > To: Histonetliste; EliMarGo@aol.com > Subject: Re: [Histonet] sectioning > > We use only slide microtomes for routine histology. I am working as a > histotech for ten years. > My average speed is 10 slides in 10-15 min (one cut on one slide); > and 10 slides in 20-25 min (4 cuts on one slide, biopsies) > We (2-3 persons) cut every day from 7 to 11. And each person does ca. > 80-100 > slides to manage the extent of one day. > After this we are happy to stand up. I cannot believe, that anyone cuts > eight hours a day without any troubles. (on body and mind) > > Gudrun Lang > Linz, Austria > > ----- Original Message ----- > From: > To: > Sent: Friday, December 19, 2003 9:44 PM > Subject: [Histonet] sectioning > > > > Hello everyone, > > > > This is the first time I use histonet and I hope I do it right.:) I > have a > pretty general question and hope you can help me with it. Can anyone > tell me > what is the average number of slides one tech. can section per an 8 hour > day? > > > > I was recently asked this question by my boss and truthfully I can > produce > 250+ slides for serial cuts, more if it is regular sectioning. The > reason I > am asking is because I am curious to know if this is the norm or if I > have > to work on my speed. I also have to add that I have only been doing this > type of work for about 1.5 years now.Hope someone can give me the > information I seek. > > > > Happy Holidays everyone!! > > > > > > Sincerely, > > > > Elisa Gonzalez > > Research Tech. II > > Doheny Eye Institute > > Pathology Department > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From EliMarGo <@t> aol.com Sun Dec 21 11:01:59 2003 From: EliMarGo <@t> aol.com (EliMarGo@aol.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Re: Sectioning Message-ID: <17b.2387cde9.2d172c07@aol.com> Thank you all for your responses. I agree with all of you in that sectioning for the 8 hour period is tiresome and hard on my back at times, this last fact being especially unnerving since I am only 26.:) I also appreciate all the advice and tips I have received in terms of quality vs. speed. This I will follow religiously and hope that my boss appreciates the difference. We at Doheny concentrate mainly on ocular biopsies i.e., globes, lagrimals, etc. So, it is difficult to try and increase speed, especially with the globes. Once again thank you all very much and if there is any other tips that you can share in the future please let me know. I will appreciate all and any help extended. Sincerely, Elisa Gonzalez Research Tech. II Doheny Eye Institute Pathology Department -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031221/a60757ed/attachment.htm From L.Driessen <@t> orthop.umcn.nl Mon Dec 22 00:03:53 2003 From: L.Driessen <@t> orthop.umcn.nl (Driessen, L.) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: <5B31B9F59E138A409E8F7C2183FF36F0040903@umcnet13.umcn.nl> -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: zondag 21 december 2003 19:00 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 1, Issue 181 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Sectioning (EliMarGo@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Sun, 21 Dec 2003 12:01:59 EST From: EliMarGo@aol.com Subject: [Histonet] Re: Sectioning To: bradbury.bc@shaw.ca, histonet@pathology.swmed.edu Message-ID: <17b.2387cde9.2d172c07@aol.com> Content-Type: text/plain; charset="us-ascii" Thank you all for your responses. I agree with all of you in that sectioning for the 8 hour period is tiresome and hard on my back at times, this last fact being especially unnerving since I am only 26.:) I also appreciate all the advice and tips I have received in terms of quality vs. speed. This I will follow religiously and hope that my boss appreciates the difference. We at Doheny concentrate mainly on ocular biopsies i.e., globes, lagrimals, etc. So, it is difficult to try and increase speed, especially with the globes. Once again thank you all very much and if there is any other tips that you can share in the future please let me know. I will appreciate all and any help extended. Sincerely, Elisa Gonzalez Research Tech. II Doheny Eye Institute Pathology Department -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031221/a60757ed/attachment-0001.htm ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 1, Issue 181 **************************************** From hborgeri <@t> wfubmc.edu Mon Dec 22 07:43:22 2003 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Unsubscribe Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8E9C8@EXCHVS2.medctr.ad.wfubmc.edu> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 3781 bytes Desc: image001.jpg Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031222/9cea3b7e/attachment.jpg -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 15492 bytes Desc: image002.gif Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031222/9cea3b7e/attachment.gif From Stephen.Eyres <@t> sanofi-synthelabo.com Mon Dec 22 08:06:33 2003 From: Stephen.Eyres <@t> sanofi-synthelabo.com (Stephen.Eyres@sanofi-synthelabo.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Qualifiaction of Analytical Instruments for use in the Pharmaceutical Industry Message-ID: Hi, Just wondered in histologists working in the Pharm industry had seen the above documented and had thoughts on issues with compliance. If you haven't seen it, let me know and I'll send a copy. Cheers Steve From louri_c <@t> hotmail.com Mon Dec 22 14:33:36 2003 From: louri_c <@t> hotmail.com (Louri Caldwell) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Cost Containment - Blades question Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031222/db7fdc04/attachment.htm From GrobeA <@t> saintpatrick.org Mon Dec 22 14:47:26 2003 From: GrobeA <@t> saintpatrick.org (Albert Grobe) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Looking for a used Cryostat Message-ID: Fellow Histonet Folks, I have been asked to find a used cryostat for a vascular research lab that is in the final phase of constuction. If you have one available to purchase, please feel free to send me an e-mail at: GrobeA@saintpatrick.org Please include make and model, and a price (if available) and I will pass on the information to the lab director. Thanks, Albert From hsvlle <@t> free.net.nz Mon Dec 22 14:43:49 2003 From: hsvlle <@t> free.net.nz (Lorraine Rolston) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Christmas wishes. Message-ID: <3FE75785.00000A.58839@default> Dear everyone in histoland. Best Wishes for a Very Merry Christmas and a Happy New Year from down-under New Zealand!!!!! God bless you all. Histonet is my favourite email. Cheers, Lorraine. From RossS <@t> BaylorHealth.edu Mon Dec 22 15:31:58 2003 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Cost Containment - Blades question Message-ID: Louri: My answers are below. "Have any of you used steel blades for paraffin sectioning instead of disposable blades?" I have never used the steel blades. Sharpening them is an art and takes time and if you don't have someone on staff who knows how to sharpen blades properly then no one will be getting good sections. "Is this practical - meaning how many blocks is one blade able to section before being sharpened, and what is the minimum cost involved in the sharpening process?" I don't believe it is practical. How many blocks you can cut depends on the type of tissue. One calcified area or staple will immediately make that area of the blade useless. As for cost. Blade sharpeners are not cheap, plus there is the time involved for the person doing the sharpening. "Is there any way to sharpen disposable blades?" Not that I know of. What is the average amount of blocks a technician is able to cut/blade - both using disposable and steel? Once again it depends on the tissue and the expected level of quality in the lab. As far as I am concerned slides should always be as close to perfect as possible. I have seen some "factory labs" who will dole out how many blades each tech gets for the day. Their slides look terrible and any Pathologist with a choice eventually look elsewhere for quality work. In my opinion quality needs to be the first priority. These are patients whose lives depend on an accurate diagnosis. They deserve no less than the best section possible. "What is your disposable blade of choice?" Accu Edge. Although I had some sample Shandon Blades recently that were good and I intend to give them some consideration as they are much cheaper than Accu Edge. Bottom Line. Unless you have a bunch of good old steel blades, a good sharpener, and a group of techs who are able to sharpen them properly then I would stick with disposables. It is much better to look at cheaper disposable blades to save money than to try to dictate how many blades each person can use. Any switch to a cheaper blade should involve the techs input. Many times blades are cheaper for a reason and they don't cut as well. Good luck. I know situations like this can be tough. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From pmarcum <@t> polysciences.com Mon Dec 22 15:36:25 2003 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Cost Containment - Blades question In-Reply-To: Message-ID: <001601c3c8d3$a6616040$4800a8c0@PMARCUM2K> Great Answer!! I have used steel blades and for some things in research they are very good however, each point you made in your summary is a consideration to be evaluated. I think we should all whether industry or clinical, supplier or end user take your last paragraph and remember the patient is our priority for everything. When I think I am losing sight of that fact I think of my daughter having surgery or other family member or friend and what I would want for them. That puts it in perspective 'cause when its personal only the best is good enough. Best Regards, Pamela A Marcum Histology/Microscopy Product Development Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Telephone: 800-523-2575???? Ext. 167 215-343-6484???? Ext. 167 Fax:? 800-343-3291 ???????? 215-343-0214 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Stapf, > Ross > Sent: Monday, December 22, 2003 4:32 PM > To: Louri Caldwell; histonet@pathology.swmed.edu > Subject: RE: [Histonet] Cost Containment - Blades question > > > > Louri: > My answers are below. > > "Have any of you used steel blades for paraffin sectioning instead of > disposable blades?" > I have never used the steel blades. Sharpening them is an art and takes > time and if you don't have someone on staff who knows how to sharpen > blades properly then no one will be getting good sections. > > "Is this practical - meaning how many blocks is one blade able to > section before being sharpened, and what is the minimum cost involved in > the sharpening process?" > I don't believe it is practical. How many blocks you can cut depends on > the type of tissue. One calcified area or staple will immediately make > that area of the blade useless. As for cost. Blade sharpeners are not > cheap, plus there is the time involved for the person doing the > sharpening. > > "Is there any way to sharpen disposable blades?" > Not that I know of. > > What is the average amount of blocks a technician is able to cut/blade - > both using disposable and steel? > Once again it depends on the tissue and the expected level of quality in > the lab. As far as I am concerned slides should always be as close to > perfect as possible. I have seen some "factory labs" who will dole out > how many blades each tech gets for the day. Their slides look terrible > and any Pathologist with a choice eventually look elsewhere for quality > work. In my opinion quality needs to be the first priority. These are > patients whose lives depend on an accurate diagnosis. They deserve no > less than the best section possible. > > "What is your disposable blade of choice?" > > Accu Edge. Although I had some sample Shandon Blades recently that were > good and I intend to give them some consideration as they are much > cheaper than Accu Edge. > > Bottom Line. Unless you have a bunch of good old steel blades, a good > sharpener, and a group of techs who are able to sharpen them properly > then I would stick with disposables. It is much better to look at > cheaper disposable blades to save money than to try to dictate how many > blades each person can use. Any switch to a cheaper blade should > involve the techs input. Many times blades are cheaper for a reason and > they don't cut as well. > > Good luck. I know situations like this can be tough. > > Ross M Stapf > Histopathology Manager > Baylor University Medical Center > 3500 Gaston Ave. > Dallas, TX 75246 > 214-820-2465 > 214-820-4110 fax > RossS@baylorhealth.edu > > > This e-mail, facsimile, or letter and any files or attachments > transmitted with it contains information that is confidential and > privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are > the intended recipient, further disclosures are prohibited > without proper authorization. If you are not the intended > recipient, any disclosure, copying, printing, or use of this > information is strictly prohibited and possibly a violation of > federal or state law and regulations. If you have received this > information in error, please notify Baylor Health Care System > immediately at 1-866-402-1661 or via e-mail at > privacy@baylorhealth.edu. Baylor Health Care System, its > subsidiaries, and affiliates hereby claim all applicable > privileges related to this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegg <@t> colobio.com Mon Dec 22 16:17:36 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Looking for a used Cryostat In-Reply-To: Message-ID: I just got a flyer from a company called Pathology Equipment Xchange that sells refurbished equipment, don't know them or endorse them just thought I would pass this on www.pathx.com 815-395-8311 inquiry@pathx.com Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Albert Grobe Sent: Monday, December 22, 2003 1:47 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Looking for a used Cryostat Fellow Histonet Folks, I have been asked to find a used cryostat for a vascular research lab that is in the final phase of constuction. If you have one available to purchase, please feel free to send me an e-mail at: GrobeA@saintpatrick.org Please include make and model, and a price (if available) and I will pass on the information to the lab director. Thanks, Albert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hborgeri <@t> wfubmc.edu Mon Dec 22 16:18:32 2003 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Unsubscribe Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8E9CC@EXCHVS2.medctr.ad.wfubmc.edu> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 3781 bytes Desc: image001.jpg Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031222/87148278/attachment.jpg -------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 15492 bytes Desc: image002.gif Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031222/87148278/attachment.gif From JpGar1109 <@t> cs.com Mon Dec 22 19:01:03 2003 From: JpGar1109 <@t> cs.com (JpGar1109@cs.com) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: <1d2.169833c4.2d18edcf@cs.com> In a message dated 12/22/03 1:02:44 AM Eastern Standard Time, L.Driessen@orthop.umcn.nl writes: << histonet@lists.utsouthwestern.edu >> From tpmorken <@t> labvision.com Mon Dec 22 19:14:54 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CE6A@usca0082k03.rallansci.apogent.com> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031222/ba0c9a18/attachment.htm From ekaplan <@t> squ.edu.om Mon Dec 22 22:29:55 2003 From: ekaplan <@t> squ.edu.om (Evelyn Kaplan) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] Christmas greetings Message-ID: Wishing the histonet a very merry Christmas and a happy New Year 2004 from here in the Gulf of Arabia. It is very special to have the backup of the histonet and long may it continue. Thanks to all who make it happen!! Hope you all have a peaceful and joyous time! Evelyn Kaplan, Dept of Pathology, College of Medicine and Health Sciences, Sultan Qaboos University, Oman From Mandy <@t> serotec.co.uk Tue Dec 23 02:32:17 2003 From: Mandy <@t> serotec.co.uk (Mandy Townsend) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: Mandy Townsend MSc Technical Services Supervisor Serotec Ltd 22 Bankside Station Approach Kidlington Oxfordshire OX5 1JE Tel: +44 1865 852736 Fax: +44 1865 852739 email: mandy@serotec.co.uk URL: www.serotec.com Serotec-Your first choice for antibodies! IMPORTANT NOTICE: This message and any attachments may be confidential. If this has been sent to you in error, please contact the sender as soon as possible. Serotec Ltd. Registered in England No.1604642 Registered Office: Boswell House, 1-5 Broad Street, Oxford, OX1 SAW. UK -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031223/974f24e4/attachment.htm From charles.lawrie <@t> ndcls.ox.ac.uk Tue Dec 23 03:15:52 2003 From: charles.lawrie <@t> ndcls.ox.ac.uk (Charles Lawrie) Date: Fri Sep 16 15:22:22 2005 Subject: [Histonet] unsubscribe Message-ID: [Histonet] unsubscribe Dr. Charles H. Lawrie, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, LRF Immunodiagnostics Unit, Room 5501, Level 5, John Radcliffe Hospital, Oxford. OX3 9DU. Tel: (01865) 222197 e-mail: charles.lawrie@ndcls.ox.ac.uk -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Lawrie, Charles N:Lawrie;Charles ORG:;Nuffield Department of Clinical Laboratory Sciences EMAIL;WORK;PREF;NGW:clawrie@gwmail.jr2.ox.ac.uk END:VCARD From M.Bromley <@t> dgri.scot.nhs.uk Tue Dec 23 05:22:07 2003 From: M.Bromley <@t> dgri.scot.nhs.uk (Mike Bromley) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Superfrost plus slides Message-ID: <7325D637DFE2D211928800902733A7F303B949FC@DGAMTBDCEMS> Hi All We have had a problem with a batch of Menzel Superfrost Plus slides (3451272). They have been made in a way that makes them more hydrophobic than usual. The problem may not have been noticed by others because it only occurs under certain circumstances, however the consequences can be extremely serious. The circumstances are: 1. Use of a "flatbed" stainer where the slides are held horizontally 2. Use of a bad batch of slides 3. Use of these slides for antibodies which do not require pressure cooking, this usually means enzyme treatment or no treatment (sometimes even when heat treated in a 95-99* water bath as in the Herceptest) Pressure cooking the slides seems to overcome the hydrophobic nature of the "bad" slides. Wash buffer usually spreads okay on the slides, but when the reagent is dripped onto the slide it fails to spread evenly. This is usually not a problem if the section is a single piece of tissue and it is hit by the drops however if the section is composed of small pieces then some of these can be missed. Hence your controls may be fine, because they are often a single piece of a reasonable size, but you may miss a critical piece of tissue. I advise everyone who uses a flatbed horizontal stainer for sections not requiring pressure cook antigen retrieval to watch the addition of the dropped reagents. This problem is due to batch variation and may be slight or extreme depending on the batch. Other users have noted problems with Superfrost Plus slides before (see histonet archives) We use Superfrost Plus slides because they are better than home made APES slides especially for our slides that are pressure cooked in high pH solution. All our reagents are from Dako and the Autostainer protocol is followed. Best Wishes Mike Bromley Chief Biomedical Scientist Pathology Dumfries & Galloway Royal Infirmary Scotland, UK > This e-mail and any files transmitted with it are private and intended > solely for the use of the individual or entity to whom they are addressed. > If you have received this e-mail in error please return it to the address > it > came from telling them it is not for you and then delete it from your > system. > > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031223/a440a065/attachment.htm From nick.kirk3 <@t> btopenworld.com Tue Dec 23 07:33:20 2003 From: nick.kirk3 <@t> btopenworld.com (nick.kirk3@btopenworld.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Superfrost plus slides Message-ID: <5026977.1072186400988.JavaMail.root@127.0.0.1> Hi Mike We've have a similar problem on occasions with Surgipath slides as well. We also have the DAKO Autostainer and use DAKO diluent and detection reagents. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England > from: Mike Bromley > date: Tue, 23 Dec 2003 11:22:07 > to: scoticc@bigfoot.com, dodson@liv.ac.uk, histonet@lists.utsouthwestern.edu, suzy.howard@dakocytomation.co.uk, katrina.murphy@dakocytomation.co.uk > subject: Re: [Histonet] Superfrost plus slides > > Hi All > > We have had a problem with a batch of Menzel Superfrost Plus slides > (3451272). They have been made in a way that makes them more hydrophobic > than usual. > > The problem may not have been noticed by others because it only occurs under > certain circumstances, however the consequences can be extremely serious. > > The circumstances are: > 1. Use of a "flatbed" stainer where the slides are held horizontally > 2. Use of a bad batch of slides > 3. Use of these slides for antibodies which do not require pressure > cooking, this usually means enzyme treatment or no treatment (sometimes even > when heat treated in a 95-99* water bath as in the Herceptest) Pressure > cooking the slides seems to overcome the hydrophobic nature of the "bad" > slides. > > Wash buffer usually spreads okay on the slides, but when the reagent is > dripped onto the slide it fails to spread evenly. This is usually not a > problem if the section is a single piece of tissue and it is hit by the > drops however if the section is composed of small pieces then some of these > can be missed. Hence your controls may be fine, because they are often a > single piece of a reasonable size, but you may miss a critical piece of > tissue. > > I advise everyone who uses a flatbed horizontal stainer for sections not > requiring pressure cook antigen retrieval to watch the addition of the > dropped reagents. This problem is due to batch variation and may be slight > or extreme depending on the batch. > > Other users have noted problems with Superfrost Plus slides before (see > histonet archives) We use Superfrost Plus slides because they are better > than home made APES slides especially for our slides that are pressure > cooked in high pH solution. > > All our reagents are from Dako and the Autostainer protocol is followed. > > Best Wishes > > Mike Bromley > > Chief Biomedical Scientist > Pathology > Dumfries & Galloway Royal Infirmary > Scotland, UK > > > This e-mail and any files transmitted with it are private and intended > > solely for the use of the individual or entity to whom they are addressed. > > If you have received this e-mail in error please return it to the address > > it > > came from telling them it is not for you and then delete it from your > > system. > > > > From rschoon <@t> email.unc.edu Tue Dec 23 07:33:24 2003 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Cost Containment - Blades question References: Message-ID: <3FE84424.7070002@email.unc.edu> Lauri, Having used both as when I started in Histology there were no disposable blades until the first one came out (Phil Picket's design), and having worked on the design and manufacture of a certain companies' blade when I worked in the corporate world my answers are as follows: 1- Have any of you used steel blades for paraffin sectioning instead of disposable blades? - Yes, but see #2 below 2 - Is this practical - meaning how many blocks is one blade able to section before being sharpened, and what is the minimum cost involved in the sharpening process? - Of course it is practical, they were used for decades prior to the advent of disposable blades. The number of blocks and slides that were able to be cut from either type of blade is dependent on the type of tissue, staples, and the technician. Quality should always come before quantity. As for cost the following figures come from memory of about 20 years ago when I ran a large hospital histology lab in MI. A minimum of 3 knives per tech at $250.00 (1 was always being sharpened). Autosharp IV or V knife sharpener at 4-5 K each, I had 3 in my lab. You could buy cheaper but you get what you pay for. Supplies for the sharpeners such as extra plates and dressing compound runs into the hundreds of dollars. Also the knives after time would still have to be sent out for reconditioning at about $100.00 a pop. Now the reason for the preceding is that I had a lab with 8 techs and 2-3 students and a large variance in hand sharpening skills. A good hand sharpened blade requires skill and time but comparatively little equipment cost. For all of the preceding there is a fair amount of tech time involved, now days better used for other things. 3 - Is there any way to sharpen disposable blades? - Let's not be ridiculous, there is a reason for the word "disposable". 4 - What is the average amount of blocks a technician is able to cut/blade - both using disposable and steel? - see #2 above 5 - The best disposable blade is the one that produces the best sections for YOU. You'll be able to find techs that will swear by any manufacturers' blade and they will all be right because that "blade " works for them. Robert Schoonhoven, From froyer <@t> bitstream.net Tue Dec 23 09:11:59 2003 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Looking for a used Cryostat In-Reply-To: References: Message-ID: <3FE85B3F.1010007@bitstream.net> Contact: Ford Royer, MT(ASCP) 27 years experience in Histology Equipment Sales. 12 years experience in Refurbished Equipment Sales & Service Analytical Instruments, llc 1200 Mendelssohn Ave. N., ste. 50 Minneapolis, MN 55427 (800) 565-1895, ext. 17 FAX: (952) 929-1895 Email: Albert Grobe wrote: >Fellow Histonet Folks, >I have been asked to find a used cryostat for a vascular research lab that is in the final phase of constuction. If you have one available to purchase, please feel free to send me an e-mail at: >GrobeA@saintpatrick.org > >Please include make and model, and a price (if available) and I will pass on the information to the lab director. > >Thanks, >Albert > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From froyer <@t> bitstream.net Tue Dec 23 09:12:11 2003 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Looking for a used Cryostat In-Reply-To: References: Message-ID: <3FE85B4B.2080703@bitstream.net> Contact: Ford Royer, MT(ASCP) 27 years experience in Histology Equipment Sales. 12 years experience in Refurbished Equipment Sales & Service Analytical Instruments, llc 1200 Mendelssohn Ave. N., ste. 50 Minneapolis, MN 55427 (800) 565-1895, ext. 17 FAX: (952) 929-1895 Email: Albert Grobe wrote: >Fellow Histonet Folks, >I have been asked to find a used cryostat for a vascular research lab that is in the final phase of constuction. If you have one available to purchase, please feel free to send me an e-mail at: >GrobeA@saintpatrick.org > >Please include make and model, and a price (if available) and I will pass on the information to the lab director. > >Thanks, >Albert > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From nmuvarak <@t> facstaff.wisc.edu Tue Dec 23 09:11:54 2003 From: nmuvarak <@t> facstaff.wisc.edu (NIDAL E MUVARAK) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Superfrost plus slides Message-ID: <101f1310516f.10516f101f13@wiscmail.wisc.edu> I've had problems with the last 100 slides or so I got from Fisher (the superfrost plus). Sections didn't even make it through fixation in aceton. I didn't have this problem before, just with the last box of slides I was using. I'm guessing it's just a bad batch. If there's a different reason for that, I'd be grateful if someone can provide some information on that. Thank you and happy holidays. Nidal E Muvarak Associate Research Specialist Vascular Tissue Biomechanics Laboratory Department of Biomedical Engineering University of Wisconsin-Madison 1550 Engineering Dr.; Rm. 2158 Madison, WI 53706-1609 Lab: (608) 265-8921; Office: (608) 265-4205; Home: (608) 256-7934; Cell: (608) 332-6068 http://vtb.bme.wisc.edu ----- Original Message ----- From: nick.kirk3@btopenworld.com Date: Tuesday, December 23, 2003 7:33 am Subject: Re: [Histonet] Superfrost plus slides > Hi Mike > > We've have a similar problem on occasions with Surgipath slides as > well. We also have the DAKO Autostainer and use DAKO diluent and > detection reagents. > > Nick Kirk > Histopathology > Hinchingbrooke Hospital > Huntingdon > England > > > from: Mike Bromley > > date: Tue, 23 Dec 2003 11:22:07 > > to: scoticc@bigfoot.com, dodson@liv.ac.uk, > histonet@lists.utsouthwestern.edu, suzy.howard@dakocytomation.co.uk, katrina.murphy@dakocytomation.co.uk > > subject: Re: [Histonet] Superfrost plus slides > > > > Hi All > > > > We have had a problem with a batch of Menzel Superfrost Plus slides > > (3451272). They have been made in a way that makes them more > hydrophobic> than usual. > > > > The problem may not have been noticed by others because it only > occurs under > > certain circumstances, however the consequences can be extremely > serious.> > > The circumstances are: > > 1. Use of a "flatbed" stainer where the slides are held horizontally > > 2. Use of a bad batch of slides > > 3. Use of these slides for antibodies which do not require pressure > > cooking, this usually means enzyme treatment or no treatment > (sometimes even > > when heat treated in a 95-99* water bath as in the Herceptest) > Pressure> cooking the slides seems to overcome the hydrophobic > nature of the "bad" > > slides. > > > > Wash buffer usually spreads okay on the slides, but when the > reagent is > > dripped onto the slide it fails to spread evenly. This is > usually not a > > problem if the section is a single piece of tissue and it is hit > by the > > drops however if the section is composed of small pieces then > some of these > > can be missed. Hence your controls may be fine, because they are > often a > > single piece of a reasonable size, but you may miss a critical > piece of > > tissue. > > > > I advise everyone who uses a flatbed horizontal stainer for > sections not > > requiring pressure cook antigen retrieval to watch the addition > of the > > dropped reagents. This problem is due to batch variation and may > be slight > > or extreme depending on the batch. > > > > Other users have noted problems with Superfrost Plus slides > before (see > > histonet archives) We use Superfrost Plus slides because they > are better > > than home made APES slides especially for our slides that are > pressure> cooked in high pH solution. > > > > All our reagents are from Dako and the Autostainer protocol is > followed.> > > Best Wishes > > > > Mike Bromley > > > > Chief Biomedical Scientist > > Pathology > > Dumfries & Galloway Royal Infirmary > > Scotland, UK > > > > > This e-mail and any files transmitted with it are private and > intended> > solely for the use of the individual or entity to whom > they are addressed. > > > If you have received this e-mail in error please return it to > the address > > > it > > > came from telling them it is not for you and then delete it > from your > > > system. > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From STapper <@t> slhduluth.com Tue Dec 23 09:44:37 2003 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] this is a test - please delete! Message-ID: <3BFBBD68413CB443A7125A63EC0ACD1D33A519@slhw2smail01.slhdomain.com> This is a test message. CONFIDENTIAL: The confidential information accompanying this transmission may contain protected health information and is legally privileged under state and federal law. This information is intended only for the use of the individual to which it is addressed. The recipient or person responsible for delivering this information is prohibited by law from disclosing this information without proper authorization to any other party, unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. No response indicates that the information was received by the appropriate authorized party. Warning: Although St. Luke's has taken reasonable precautions to ensure no viruses are present in this email, St. Luke's cannot accept responsibility for any loss or damage arising from the use of this email or attachments. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031223/d7108189/attachment.htm From livieira <@t> ualg.pt Tue Dec 23 10:47:51 2003 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Christmas wishes Message-ID: <003801c3c974$811656a0$2914100a@labhistologia> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/gif Size: 774 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031223/2ff4bc04/attachment.gif From BMolinari <@t> heart.thi.tmc.edu Tue Dec 23 11:19:39 2003 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] unsubscribe Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031223/6e3df5c4/attachment.htm From jdbickel <@t> statlab.com Tue Dec 23 12:54:24 2003 From: jdbickel <@t> statlab.com (John) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] unsubscribe Message-ID: <003f01c3c986$2eadd250$cc03a8c0@statlab.com> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 7798 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031223/a5286642/attachment.jpg From bills <@t> icpmr.wsahs.nsw.gov.au Tue Dec 23 14:50:26 2003 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Superfrost plus slides In-Reply-To: <5026977.1072186400988.JavaMail.root@wsahs.nsw.gov.au> Message-ID: <002a01c3c996$64fff800$3187080a@wsahs.nsw.gov.au> Merry Christmas to all from down under in Australia. We to have had problems but only with limited numbers of slides. We were told that there was "a" batch of Superfrost Slides which were suspect. I found out that nearly all Superfrost Slides are manufactured in one or two factories in Germany and packaged for the local distributors. The company claimed they knew nothing about problems with any of their slides, however there were two batches which gave problems one was # 3421272 and I believe the other also ended in 72. This happened about 3-4 months ago now and I have noticed that all the batches have expiry dates on them. >From correspondence on Histonet it appears that the problem or something like it has been going on for several months. But as is usual in Histotechnology everyone tries to solve the problem in-house without discussing it with anyone else. This is where I find Histonet invaluable. Bill Sinai Laboratory Manager Tissue Pathology, IMPAR Westmead NSW 2145 Australia Ph 02 9845 7774 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of nick.kirk3@btopenworld.com Sent: Wednesday, 24 December 2003 12:33 AM To: M.Bromley@dgri.scot.nhs.uk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Superfrost plus slides Hi Mike We've have a similar problem on occasions with Surgipath slides as well. We also have the DAKO Autostainer and use DAKO diluent and detection reagents. Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England > from: Mike Bromley > date: Tue, 23 Dec 2003 11:22:07 > to: scoticc@bigfoot.com, dodson@liv.ac.uk, histonet@lists.utsouthwestern.edu, suzy.howard@dakocytomation.co.uk, katrina.murphy@dakocytomation.co.uk > subject: Re: [Histonet] Superfrost plus slides > > Hi All > > We have had a problem with a batch of Menzel Superfrost Plus slides > (3451272). They have been made in a way that makes them more hydrophobic > than usual. > > The problem may not have been noticed by others because it only occurs under > certain circumstances, however the consequences can be extremely serious. > > The circumstances are: > 1. Use of a "flatbed" stainer where the slides are held horizontally > 2. Use of a bad batch of slides > 3. Use of these slides for antibodies which do not require pressure > cooking, this usually means enzyme treatment or no treatment (sometimes even > when heat treated in a 95-99* water bath as in the Herceptest) Pressure > cooking the slides seems to overcome the hydrophobic nature of the "bad" > slides. > > Wash buffer usually spreads okay on the slides, but when the reagent is > dripped onto the slide it fails to spread evenly. This is usually not a > problem if the section is a single piece of tissue and it is hit by the > drops however if the section is composed of small pieces then some of these > can be missed. Hence your controls may be fine, because they are often a > single piece of a reasonable size, but you may miss a critical piece of > tissue. > > I advise everyone who uses a flatbed horizontal stainer for sections not > requiring pressure cook antigen retrieval to watch the addition of the > dropped reagents. This problem is due to batch variation and may be slight > or extreme depending on the batch. > > Other users have noted problems with Superfrost Plus slides before (see > histonet archives) We use Superfrost Plus slides because they are better > than home made APES slides especially for our slides that are pressure > cooked in high pH solution. > > All our reagents are from Dako and the Autostainer protocol is followed. > > Best Wishes > > Mike Bromley > > Chief Biomedical Scientist > Pathology > Dumfries & Galloway Royal Infirmary > Scotland, UK > > > This e-mail and any files transmitted with it are private and intended > > solely for the use of the individual or entity to whom they are addressed. > > If you have received this e-mail in error please return it to the address > > it > > came from telling them it is not for you and then delete it from your > > system. > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From bills <@t> icpmr.wsahs.nsw.gov.au Tue Dec 23 14:54:16 2003 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Controls for COD Message-ID: <002b01c3c996$edcf9eb0$3187080a@wsahs.nsw.gov.au> Dear All, We have been looking for a control for C4D (Hyper Acute Rejection). In most cases the material we have is limited in volume and so we consistently run out of controls. Do any of you have suggestions what we could use as control material. So far it has been suggested tonsil may suffice but apparently the staining is inconsistent. Many thanks Bill Sinai Laboratory Manager Tissue Pathology, IMPAR Westmead NSW 2145 Australia Ph 02 9845 7774 __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From haldana <@t> unimoron.edu.ar Tue Dec 23 16:58:13 2003 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Hel with eyes References: Message-ID: <00b801c3c9a8$3e774b40$7504a8c0@um.edu> Dear Histoneters I need advaices to process rat eyes. I perfuse the animals with paraformaldehide in fosfate buffer and then I remove the lens because I need to incluede the eyes in paraffin. When I see the final sectios the retin is always detached from the tunica vascularis or media. Please help me Thank you very much. Best Wishes for a Very Merry Christmas and a Happy New Year from southamerica Argentina Visit Us is a cheap and safe country??? Patagonia, Iguazy falls, Buenos Aires night and city and the people are unforgattable... Dr. Hern?n J. Aldana Marcos Facultad de Medicina. Universidad de Mor?n Machado 914. B1708JPD. Buenos Aires. Argentina e-mail alternativo hernanjavier@yahoo.com web: http://hjaldanamarcos.bravepages.com http://histologia.bigthicketdirectory.net/main.html ----- Original Message ----- From: "Stapf, Ross" To: "Louri Caldwell" ; Sent: Monday, December 22, 2003 6:31 PM Subject: RE: [Histonet] Cost Containment - Blades question Louri: My answers are below. "Have any of you used steel blades for paraffin sectioning instead of disposable blades?" I have never used the steel blades. Sharpening them is an art and takes time and if you don't have someone on staff who knows how to sharpen blades properly then no one will be getting good sections. "Is this practical - meaning how many blocks is one blade able to section before being sharpened, and what is the minimum cost involved in the sharpening process?" I don't believe it is practical. How many blocks you can cut depends on the type of tissue. One calcified area or staple will immediately make that area of the blade useless. As for cost. Blade sharpeners are not cheap, plus there is the time involved for the person doing the sharpening. "Is there any way to sharpen disposable blades?" Not that I know of. What is the average amount of blocks a technician is able to cut/blade - both using disposable and steel? Once again it depends on the tissue and the expected level of quality in the lab. As far as I am concerned slides should always be as close to perfect as possible. I have seen some "factory labs" who will dole out how many blades each tech gets for the day. Their slides look terrible and any Pathologist with a choice eventually look elsewhere for quality work. In my opinion quality needs to be the first priority. These are patients whose lives depend on an accurate diagnosis. They deserve no less than the best section possible. "What is your disposable blade of choice?" Accu Edge. Although I had some sample Shandon Blades recently that were good and I intend to give them some consideration as they are much cheaper than Accu Edge. Bottom Line. Unless you have a bunch of good old steel blades, a good sharpener, and a group of techs who are able to sharpen them properly then I would stick with disposables. It is much better to look at cheaper disposable blades to save money than to try to dictate how many blades each person can use. Any switch to a cheaper blade should involve the techs input. Many times blades are cheaper for a reason and they don't cut as well. Good luck. I know situations like this can be tough. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tigersnake <@t> ecybermind.net Tue Dec 23 22:12:53 2003 From: tigersnake <@t> ecybermind.net (Paul Howard Lockwood) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Christmas wishes Message-ID: <200312240109.hBO19bW06083@ginsberg.ecybermind.net> To All, The best to you this Holiday. Sincerely, Paul Lockwood From whatisdog <@t> ksvcp.com Tue Dec 23 23:47:02 2003 From: whatisdog <@t> ksvcp.com (ulso) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] optimal fixative for cytology smear? Message-ID: <001801c3c9e1$5b9d35c0$1b3f2e93@ksvcper> Hi, histolanders! I am looking for adequate fixative appliable to cytology smear. In fact, I have tried acetone 100% 5 min so far, but repeatedly, I have found this fixative have poorly preserved the nuclear morphology, and somewhat dirty backgroud from celluar debris from fixation process. My topic is usually, CD antibodies such as CD3, 79a, 45RA, 1, 4, and 8 on canine lymph node or blood cells. Anyone out there recommend me any fixatives or tips on it? Literature based information would also be appreciated. Thank you and happy hollidays! Ul Soo Choi. DMV. graduate student Dept of clinical patholoy, CVM, Seoul Nat'l Univ. Seoul, Korea. From IKirbis <@t> onko-i.si Wed Dec 24 00:07:45 2003 From: IKirbis <@t> onko-i.si (IKIRBIS) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] optimal fixative for cytology smear? Message-ID: if you are asking about fixative for immuno on cytology slides I suggest you methanol, slides have to be fixed immediately in cold methanol and they should also stay in methanol, they shouldn't be dried, you can store them up to 1 month for majority of markers with one exception - tDt, you have to stain it next day hope this help, wish you happy holidays Irena Kirbis Cytopathology< department Ljubljana, Slovenia -----Original Message----- From: ulso [mailto:whatisdog@ksvcp.com] Sent: Wednesday, December 24, 2003 6:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] optimal fixative for cytology smear? Hi, histolanders! I am looking for adequate fixative appliable to cytology smear. In fact, I have tried acetone 100% 5 min so far, but repeatedly, I have found this fixative have poorly preserved the nuclear morphology, and somewhat dirty backgroud from celluar debris from fixation process. My topic is usually, CD antibodies such as CD3, 79a, 45RA, 1, 4, and 8 on canine lymph node or blood cells. Anyone out there recommend me any fixatives or tips on it? Literature based information would also be appreciated. Thank you and happy hollidays! Ul Soo Choi. DMV. graduate student Dept of clinical patholoy, CVM, Seoul Nat'l Univ. Seoul, Korea. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031224/2393d93d/attachment.htm From parakr <@t> dentistry.wits.ac.za Wed Dec 24 01:07:13 2003 From: parakr <@t> dentistry.wits.ac.za (Ruqayya Parak) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] (HISTONET) unscribe Message-ID: <007501c3c9ec$8eb50a40$2d318d92@wits.ac.za> Please unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031224/1cb60296/attachment.htm From parakr <@t> dentistry.wits.ac.za Wed Dec 24 01:09:50 2003 From: parakr <@t> dentistry.wits.ac.za (Ruqayya Parak) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Fw: (HISTONET) unsubscribe Message-ID: <008301c3c9ec$ec5eb380$2d318d92@wits.ac.za> ----- Original Message ----- From: Ruqayya Parak To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, December 24, 2003 9:07 AM Subject: (HISTONET) unscribe Please unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031224/a41d5138/attachment.htm From brokeponi <@t> yahoo.com Wed Dec 24 09:46:11 2003 From: brokeponi <@t> yahoo.com (Christine Baker) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Job opening in Crystal River Florida Message-ID: <20031224154611.35129.qmail@web60203.mail.yahoo.com> This is to let any interested person know that a position is available in the Histology department at Seven Rivers Regional Medical Center, In Crystal River Florida. This is a full time job . Interested persons should contact the human resources at( 352) 795-8418 Christine Baker Histology Supervisor Seven Rivers Regional Medical Center Crystal River Florida Brokeponi@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! Photos - Get your photo on the big screen in Times Square -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031224/0127754b/attachment.htm From hns3j <@t> virginia.edu Wed Dec 24 12:03:18 2003 From: hns3j <@t> virginia.edu (Hillary Simons) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Re: Histonet Digest, Vol 1, Issue 185 (Vacation) Message-ID: I will be out of the office from December 24, 2003 through January 2, 2004. I will respond to email when I return. Special Note: If you are dropping off protocols, they will not be picked up until January 2, 2004. All protocols received or picked up from the drop boxes by January 2, 2004 will be included in the January 14, 2004 meeting. Thanks, Hillary From histomjans <@t> yahoo.com Wed Dec 24 12:18:59 2003 From: histomjans <@t> yahoo.com (Melissa Jans) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] formalin neutralization (large quatities) Message-ID: <20031224181859.30743.qmail@web14914.mail.yahoo.com> Merry Christmas fellow histonetters!!! We currently neutralize our formalin in batches of ~75 gallons, about every 10 days. We use a product called Aldex, which has been working fine for us. However, due to recent price increases, I have been requested to find out if anyone has any suggestions for a neutralizer that may be cheaper (and easy) to use for such large quantities. FYI: Although it would be nice to use a formalin substitute, and not worry about formalin neutralization, this is not a direction we headed towards right now. Thank you! Melissa Jans University of Iowa Hospitals and Clinics --------------------------------- Do you Yahoo!? Yahoo! Photos - Get your photo on the big screen in Times Square -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031224/2a789c5a/attachment.htm From kerney <@t> fas.harvard.edu Wed Dec 24 13:03:05 2003 From: kerney <@t> fas.harvard.edu (Ryan Kerney) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] The right procesor for small sacle ISH Message-ID: <1072292585.3fe9e2e99b365@webmail.fas.harvard.edu> Hello everybody, The folks in my museum are looking into buying a tissue processor for paraffin embedding. We usually have relatively small sample sizes for processing, so I was thinking about one of these flow through processors. While I've used the larger Leica carousel processors for ISH samples before, I was wondering if there are any considerations I should keep in mind for a flow through system. Does anyone have any flow through systems they could recommend? Are these typically cheaper than the carousel type? Any and all comments will be appreciated. happy holidays, Ryan -- Ryan Kerney Department of Herpetology Museum of Comparative Zoology 26 Oxford St. Cambridge, MA 02138 Office: 617-496-4065 Lab: 617-496-4632 From pengbaowei <@t> 21cn.com Fri Dec 26 02:41:36 2003 From: pengbaowei <@t> 21cn.com (Baowei Peng) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] How to reduce background stain? Message-ID: <8v968773835897.06381@send1.inner-21cn.com> Hi,Histoneters I'm doing IHC on rat skeletal muscle. I got a heavy background= stain on all sections and control section without 1st AB. But= there is no stain on the control section without 2nd Ab. It looks like the 2nd Ab will react with the rat tissue. My first= Ab is derived from Mouse, and second Ab is anti-mouse. What could I do to reduce the background stain? =A1=A1=A1=A1=A1=A1 =09=09=09=09 =A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1Baowei Peng =A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1pengbaowei@21cn.com =A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A12003-12-26 From llczap <@t> bpthosp.org Fri Dec 26 10:15:03 2003 From: llczap <@t> bpthosp.org (Marron, Lori) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] p57 Message-ID: I am interested in running p57 in our lab and I was wondering if anyone could give me some info on what is the best company to use and the protocol for it? We use the Dako immunostainer and the Lsab2 kit. Thanks This message originates from Yale New Haven Health System. The information contained in this message may be privileged and confidential. If you are the intended recipient, you must maintain this message in a secure and confidential manner. If you are not the intended recipient, please notify the sender immediately and destroy this message. Thank you. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031226/d5806119/attachment.htm From amarusk1 <@t> FAIRVIEW.ORG Fri Dec 26 16:46:42 2003 From: amarusk1 <@t> FAIRVIEW.ORG (ANN MARUSKA) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] sentinel nodes Message-ID: Hi histonetters, I was wondering if any of you or your pathologists have tried the quick cytokeratin stain kit, such as the one made by Zymed, for frozen sections on sentinel nodes. If so, has it been a benefit? - and do you find it time consuming at all? Thanks for your help - and happy holidays to all! Ann Ann Maruska Fairview-University Medical Center Mpls. MN 55454 amarusk1@fairview.org 612-273-9119 The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material, including “protected health information.” If you are not the intended recipient, you are hereby notified that any review, retransmission, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please destroy and delete this message from any computer and contact us immediately by return e-mail. <<<>>> -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:ANN MARUSKA EMAIL;WORK;PREF;NGW:AMARUSK1@FAIRVIEW.ORG ORG:;LAB N:MARUSKA;ANN TEL;WORK:612-273-9119 END:VCARD From JCarpenter764 <@t> aol.com Sat Dec 27 07:19:36 2003 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] buying books Message-ID: <171.2810fbed.2d1ee0e8@aol.com> I recently have tried purchasing histotechnology examinations: the board of registry study guide from amazon....i put my order in november 9th and have not received the book. Does anyone know of somewhere else i could try and find this excellent book. thanks Jennell From amosbrooks <@t> earthlink.net Sat Dec 27 08:42:47 2003 From: amosbrooks <@t> earthlink.net (Amos Brooks) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Re: Histonet Digest, Vol 1, Issue 187 In-Reply-To: References: Message-ID: <6.0.0.22.0.20031227093128.029d4210@127.0.0.1> Hi, That really sounds like endogenous biotin. Try either a biotin block or a non biotin detection system. You can get the biotin block from several companies (I know DAKO carries it) there's also a home made blocking recipe using milk. Search the archives if you want to try the milk thing. It's been discussed here before. The non-biotin detection systems are also a good choice. You could use either alkaline phosphotase or one of the new systems that use a dextran polymer like Envision from DAKO (there are other companies that carry it but it's Saturday and my mind is currently on cartoons and cereal :-) ) Good luck, Amos Brooks At 01:00 PM 12/26/03, you wrote: >Hi,Histoneters >I'm doing IHC on rat skeletal muscle. I got a heavy background stain on >all sections and control section without 1st AB. But there is no stain on >the control section without 2nd Ab. >It looks like the 2nd Ab will react with the rat tissue. My first Ab is >derived from Mouse, and second Ab is anti-mouse. >What could I do to reduce the background stain? From JCarpenter764 <@t> aol.com Sun Dec 28 09:38:47 2003 From: JCarpenter764 <@t> aol.com (JCarpenter764@aol.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] open processors Message-ID: <8b.49463a.2d205307@aol.com> Can someone tell me the function of the vacuum on an open processor. I don't understand what purpose it serves or its function. thanks From bhewlett <@t> cogeco.ca Sun Dec 28 13:23:34 2003 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] open processors References: <8b.49463a.2d205307@aol.com> Message-ID: <000601c3cd78$1611a2d0$6400a8c0@bryanmainbox> Vacuum on an open processor is used only during infiltration with molten wax, it has no other function such as moving fluids which occurs in a closed type processor. Vacuum infiltration with molten wax dramatically reduces the time (and number of wax changes) necessary to remove the clearing solvent and hence reduces the time in hot wax, a good thing. Bryan ----- Original Message ----- From: To: Sent: Sunday, December 28, 2003 10:38 AM Subject: [Histonet] open processors > Can someone tell me the function of the vacuum on an open processor. I don't > understand what purpose it serves or its function. thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bamur <@t> alaska.net Sun Dec 28 05:48:15 2003 From: bamur <@t> alaska.net (Barbara Murray) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Filtering of H & E Message-ID: <000a01c3cd38$7bbdba00$0c8a70d1@d3b1l9> Greetings, I always filter my H & E before each use. I've noticed that this is not a common practice anymore. Does it matter if the H & E is filtered daily before each use? Thanks for your reply. Have a great day and a prosperous New Year! B.A. Murray, HT. (ASCP) The Alaska Native Medical Center Histology Dept. Anchorage, Alaska From mike.kirby <@t> nhls.ac.za Sun Dec 28 23:37:27 2003 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Test message - to see if my system is working Message-ID: <0FA1B7472216894D9B3381F87C71DE491DA608@nhlsmail.nhls.ac.za> From Myri37 <@t> aol.com Mon Dec 29 03:49:07 2003 From: Myri37 <@t> aol.com (Myri37@aol.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] preserving fixative glutarald =?iso-8859-1?q?=E9hyde?= Message-ID: <4FE025B9.761E22E6.0005167B@aol.com> Hello everyone i have prepared fixative since 4 months and kept it at 4?C Glutarald?hyde Grade I 1% paraformald?hyde 4% tampon cacodylat sodium 0.1 M pH 7.4 do you think i can use it ????? tha,k you very much for your help Myriam Baali Natural Implant From froyer <@t> bitstream.net Mon Dec 29 09:09:36 2003 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] open processors In-Reply-To: <000601c3cd78$1611a2d0$6400a8c0@bryanmainbox> References: <8b.49463a.2d205307@aol.com> <000601c3cd78$1611a2d0$6400a8c0@bryanmainbox> Message-ID: <3FF043B0.30904@bitstream.net> Vacuum also removes any trapped air bubbles on the cellular level. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instrumnets, llc Minneapolis, MN Bryan Hewlett wrote: >Vacuum on an open processor is used only during infiltration with molten >wax, it has no other function such as moving fluids which occurs in a closed >type processor. Vacuum infiltration with molten wax dramatically reduces the >time (and number of wax changes) necessary to remove the clearing solvent >and hence reduces the time in hot wax, a good thing. > >Bryan > > >----- Original Message ----- >From: >To: >Sent: Sunday, December 28, 2003 10:38 AM >Subject: [Histonet] open processors > > > > >>Can someone tell me the function of the vacuum on an open processor. I >> >> >don't > > >>understand what purpose it serves or its function. thanks >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From MAUGER <@t> email.chop.edu Mon Dec 29 09:35:47 2003 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] p57 Message-ID: Lori, Try the monoclonal from Lab Vision(Neomarkers) #MS-1062. It's called kip2 or P57. I use it at 1:150 after microwaving in 6 pH citrate buffer. I use the ABC method for staining, but LSAB should work well. Good luck, Joanne >>> "Marron, Lori" 12/26/03 11:15AM >>> I am interested in running p57 in our lab and I was wondering if anyone could give me some info on what is the best company to use and the protocol for it? We use the Dako immunostainer and the Lsab2 kit. Thanks This message originates from Yale New Haven Health System. The information contained in this message may be privileged and confidential. If you are the intended recipient, you must maintain this message in a secure and confidential manner. If you are not the intended recipient, please notify the sender immediately and destroy this message. Thank you. From MAUGER <@t> email.chop.edu Mon Dec 29 09:40:46 2003 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Re: Histonet Digest, Vol 1, Issue 187 Message-ID: What animal is your secondary made in? Try blocking in the normal serum of that animal for 10 minutes before applying primary ab. Jo >>> "Amos Brooks" 12/27/03 09:42AM >>> Hi, That really sounds like endogenous biotin. Try either a biotin block or a non biotin detection system. You can get the biotin block from several companies (I know DAKO carries it) there's also a home made blocking recipe using milk. Search the archives if you want to try the milk thing. It's been discussed here before. The non-biotin detection systems are also a good choice. You could use either alkaline phosphotase or one of the new systems that use a dextran polymer like Envision from DAKO (there are other companies that carry it but it's Saturday and my mind is currently on cartoons and cereal :-) ) Good luck, Amos Brooks At 01:00 PM 12/26/03, you wrote: >Hi,Histoneters >I'm doing IHC on rat skeletal muscle. I got a heavy background stain on >all sections and control section without 1st AB. But there is no stain on >the control section without 2nd Ab. >It looks like the 2nd Ab will react with the rat tissue. My first Ab is >derived from Mouse, and second Ab is anti-mouse. >What could I do to reduce the background stain? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ben.Shelkowsky <@t> chomp.org Mon Dec 29 09:47:14 2003 From: Ben.Shelkowsky <@t> chomp.org (Shelkowsky, Ben) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] open processors Message-ID: <384DA2BD670CE34DA8D3B60F4ED7157F4D60F5@exchsrvr.chomp.org> When we used to use the open processor, many , many years ago, we used a Tissue Tek vacuum infiltrator for 15 min. before we started embedding. 1. We removed the cassettes from the Technicon and transferred them into the vaccum apparatus. We turned on the vaccuum for 15 min. You can try it first on an experimental basis to see if it makes a difference. At the time we used it we felt we got better infiltration, in general of all the tissues. Have a Happy New Year! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ford Royer Sent: Mon 12/29/2003 7:09 AM To: Bryan Hewlett Cc: histonet@lists.utsouthwestern.edu; JCarpenter764@aol.com Subject: Re: [Histonet] open processors Vacuum also removes any trapped air bubbles on the cellular level. ~ Ford Ford M. Royer, MT(ASCP) Analytical Instrumnets, llc Minneapolis, MN Bryan Hewlett wrote: >Vacuum on an open processor is used only during infiltration with molten >wax, it has no other function such as moving fluids which occurs in a closed >type processor. Vacuum infiltration with molten wax dramatically reduces the >time (and number of wax changes) necessary to remove the clearing solvent >and hence reduces the time in hot wax, a good thing. > >Bryan > > >----- Original Message ----- >From: >To: >Sent: Sunday, December 28, 2003 10:38 AM >Subject: [Histonet] open processors > > > > >>Can someone tell me the function of the vacuum on an open processor. I >> >> >don't > > >>understand what purpose it serves or its function. thanks >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From BoozerKA <@t> pa1.ah.org Mon Dec 29 10:46:01 2003 From: BoozerKA <@t> pa1.ah.org (Kathleen Boozer) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Histology Job Message-ID: Adventist Medical Center Portland, OR 6-2:30 shift This is a peaceful, fast paced, (but not frantic), multi-tasked, small department. This shift will embed, cut, stain, IHC, and log in specimens helping the pathologist the last two hours of the shift. There are good benefits. From cycling <@t> another.com Mon Dec 29 11:30:42 2003 From: cycling <@t> another.com (cycling@another.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Filtering of H & E Message-ID: <3003478.1072719042259.JavaMail.root@172.16.100.50> With the strength of Alum in solution then the Haem may well crystalise a little each day, the eosin in alcoholic solution will dry around the edges, the crystals in haem are the ones that I was warned of when I was a lad. After five days the crud should be noticeable. Now there was a time when folk got tired of it all and reformulated the Haematoxylin, producing Gill's I think it was rather than Harris'. There was redundant/superfluous Alum, then there was not. So the answer depends upon what you are using. I was also taught that it was worth the bother to avoid bits of sections making a soup out of the solution and cross contaminating. All the best for the New Year Dave Edmondson Christie, Manchester UK -----Original Message----- >From : Barbara Murray To : histonet@lists.utsouthwestern.edu Date : 28 December 2003 11:48:15 Subject : [Histonet] Filtering of H & E Greetings, >I always filter my H & E before each use. I've noticed that this is not a common practice anymore. >Does it matter if the H & E is filtered daily before each use? >Thanks for your reply. >Have a great day and a prosperous New Year! > >B.A. Murray, HT. (ASCP) >The Alaska Native Medical Center >Histology Dept. >Anchorage, Alaska >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Personalised email by http://another.com From lpwenk <@t> covad.net Mon Dec 29 12:05:24 2003 From: lpwenk <@t> covad.net (Lee & Peggy Wenk) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] buying books References: <171.2810fbed.2d1ee0e8@aol.com> Message-ID: <000801c3ce36$5c5165a0$8732fea9@hppav> Order it directly from ASCP. If you are a ASCP member, you receive a 10% discount. https://www.ascp.org/500live/timssnet/products/tnt_products.cfm >From the ASCP webpage: Histotechnology: A Self-instructional Text 2nd Edition Price: $95.00 Author: Freida Carson, HT(ASCP), PhD Advances in histotechnology continue to expand the capabilities of the histopathology laboratory, but also require continual study by practitioners to remain current. The second edition of Histotechnology; A Self-Instructional Text presents all the latest advances as well as the fundamentals that are critical to achieving the highest quality work. Written as both reference and teaching tool Histotechnology; A Self-Instructional Text addresses the essentials of preparing histology specimens including staining and processing, laboratory safety issues, and immunohistochemistry in fourteen instructional units. Electron microscopy and enzyme histochemistry have been added to this edition to help histotechnologists preparing for the HTL(ASCP) certifying examination. Each unit presents the learning objectives of the section, explains and illustrates the technique, and then gives specific learning activities that will reinforce the information. Written as both a teaching tool and reference, the format also makes troubleshooting fast and easy. Histotechnology is an excellent learning tool for histologic technic students, and for residents and pathologists seeking to expand their understanding of the technology used in the histopathology laboratory. It is equally well suited as a desk reference for the more common special staining techniques and their results. Partial Contents Fixation; Processing; Instrumentation; Safety; Laboratory Mathematics and Solution Preparation; Nuclear and Cytoplasmic Staining; Carbohydrates and Amyloid; Connective and Muscle Tissue; Nerve; Microorganisms; Pigments, Minerals, and Cytoplasmic Granules; Immunohistochemistry; Enzyme Histochemistry; Electron Microscopy. Hardbound; 312 pages; 286 images; 6 tables; 47 figures; 1997 ISBN: 089189411X Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Saturday, December 27, 2003 8:19 AM Subject: [Histonet] buying books > I recently have tried purchasing histotechnology examinations: the board of > registry study guide from amazon....i put my order in november 9th and have not > received the book. Does anyone know of somewhere else i could try and find > this excellent book. thanks Jennell > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mprice26 <@t> juno.com Mon Dec 29 14:51:01 2003 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Minnesota Method for staining Bone Marrows' Message-ID: <20031229.125149.25056.1398144@webmail20.nyc.untd.com> Hi Histonetters, Hope everyone had a Merry Christmas and will have a Happy New Year. I need a copy of the Minnesota Method of staining Bone Marrows'. Would anyone happen to have one that they could e-mail to me or fax to me? Thank you. Marsha Price ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From pruegg <@t> colobio.com Mon Dec 29 16:45:30 2003 From: pruegg <@t> colobio.com (Patsy Ruegg) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] influenza A Message-ID: Looking for a lab that tests for "influenza A" in a paraffin tissue block, does that test exist? Thanks and Happy New Year! Patsy Ruegg From mcdonal1 <@t> ccf.org Mon Dec 29 17:13:30 2003 From: mcdonal1 <@t> ccf.org (Linda McDonald) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] CPT Code Message-ID: When doing Oil Red O stains, Gomori's Trichrome on Frzen muscle tissue or Pas on frozen muscle tissue, what CPT codes are folks using for this? Are they counted as an 88313 or would they be an 88314? Let me know what you all are doing with these. Thanks a lot and Happy New Year!!!!! LGMc From dw18 <@t> uchicago.edu Mon Dec 29 17:37:54 2003 From: dw18 <@t> uchicago.edu (David Anthony Wright) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] re: IHC background [Peng] - rat/Ms X-Rxn? Message-ID: <1072741074.3ff0bad2a07e3@webmail.uchicago.edu> Hello Baowei Peng (& Histonet) Amos Brooks has suggested the problem below might be endogenous biotin, with helpful suggestions what to do. I'd like to suggest something else if that doesn't work. A couple of questions- 1) Is your anti-mouse secondary a polyclonal serum (almost certainly) and, if so, is it not rat-adsorbed? 2) Is there any reason for there being an inflammatory reaction in your tissue? There is about a 5-10% overlap in crossreactivity between anti-mouse and anti- rat IgG antibodies (cf. goat & sheep), depending on how distant the host species is. I've used standard Vector biotinylated anti-mouse IgG secondary (BA2000) happily for IHC on rat brain for years with no background but got a huge background cross reaction in every animal when staining material 2 days after brain surgery when inflammation is extensive. I know it was X-reaction with native rat IgGs in the tissue because the problem vanished when I either i) added normal rat serum to the 2ndary incubation mix and so competed out the cross reacting moiety or ii) used Vector's rat-adsorbed secondary (BA2001). It's more expensive of course! good luck! -David Wright >Hi,Histoneters >I'm doing IHC on rat skeletal muscle. I got a heavy background stain on >all sections and control section without 1st AB. But there is no stain on >the control section without 2nd Ab. >It looks like the 2nd Ab will react with the rat tissue. My first Ab is >derived from Mouse, and second Ab is anti-mouse. >What could I do to reduce the background stain?-Baowei Peng -- David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ================================================ Does 2+2=5 for large values of 2? [YES!] From pengbaowei <@t> 21cn.com Mon Dec 29 19:52:48 2003 From: pengbaowei <@t> 21cn.com (Baowei Peng) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] How to educe back ground Message-ID: <2S968769905369.06076@send6.inner-21cn.com> Hi,all Thanks for your kindly responses. My second Ab is said to be a mAb in the description file from= manufacture. But do not say if it was absorbed by rat IgG,= problely not. David Wright is right. There is an inflammatory reaction as seen= by mononuclear cell infiltration and diapedesis in the tissue. So maybe add rat serum to blocking and antibody diluent reagent= will work. I will try this out. =09=09=09=09 =A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1Baowei Peng =A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1pengbaowei@21cn.com =A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A1=A12003-12-30 From tvedilago <@t> system1.net Tue Dec 30 08:00:50 2003 From: tvedilago <@t> system1.net (Tommy Vedilago) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Jobs in Histology Message-ID: Hello again Histonetters, We are still looking for highly qualified and energetic managers for several locations across the country. We need supervisors in Washington, D.C., Philadelphia, Maine, Houston, NYC, Tucson, AZ, Tampa, New Jersey, Nashville, and Brooklyn. We are also looking for techs in various locations and we are now accepting travelers who are tired of dealing with the large companies. Please feel free to contact me via email or at 866-797-8361. I hope all of you had a merry Christmas and have a happy New Year. Tommy Vedilago System 1 Search (864) 627-0012 (864) 627-0013 Fax From BMolinari <@t> heart.thi.tmc.edu Tue Dec 30 11:41:26 2003 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] COBr2 Message-ID: Hi histonetters, A researcher has given us a protocol he found from quite a while ago and it calls for a chemical COBr2. The chemical name is carbonyl bromide. We cannot find a supplier. Does anyone know about this chemical? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Houston, TX 77225 832-355-6524 832-355-6812 (fax) From kerney <@t> fas.harvard.edu Tue Dec 30 12:21:31 2003 From: kerney <@t> fas.harvard.edu (Ryan Kerney) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Reducing TSA background in ISH In-Reply-To: References: Message-ID: Hello out there, Does anyone have any experience with using the tyramide signal amplification in ISH? I am using it on connective tissue sections with Anti-DIG-POD --> DNP Amplification --> Anti-DNP-AP. There is a lot of background for all probe concentrations (1:1 through 1:20). I'm pretreating the tissues with H2O2. Should I use Levamisol as well? Should I block longer (using 1 hour)? Should I block before the Anti-DIG and Anti-DNP? Any suggestions will be very appreciated. -RK On Dec 30, 2003, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: buying books (Lee & Peggy Wenk) > 2. Minnesota Method for staining Bone Marrows' (mprice26@juno.com) > 3. influenza A (Patsy Ruegg) > 4. CPT Code (Linda McDonald) > 5. re: IHC background [Peng] - rat/Ms X-Rxn? (David Anthony Wright) > 6. How to educe back ground (Baowei Peng) > 7. Jobs in Histology (Tommy Vedilago) > 8. COBr2 (Molinari, Betsy) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 29 Dec 2003 13:05:24 -0500 > From: "Lee & Peggy Wenk" > Subject: Re: [Histonet] buying books > To: , > Message-ID: <000801c3ce36$5c5165a0$8732fea9@hppav> > Content-Type: text/plain; charset="iso-8859-1" > > Order it directly from ASCP. If you are a ASCP member, you receive a > 10% > discount. > > https://www.ascp.org/500live/timssnet/products/tnt_products.cfm > > >> From the ASCP webpage: > > Histotechnology: A Self-instructional Text 2nd Edition > > Price: $95.00 > > Author: Freida Carson, HT(ASCP), PhD > > Advances in histotechnology continue to expand the capabilities of the > histopathology laboratory, but also require continual study by > practitioners > to remain current. The second edition of Histotechnology; A > Self-Instructional Text presents all the latest advances as well as the > fundamentals that are critical to achieving the highest quality work. > Written as both reference and teaching tool Histotechnology; A > Self-Instructional Text addresses the essentials of preparing histology > specimens including staining and processing, laboratory safety issues, > and > immunohistochemistry in fourteen instructional units. Electron > microscopy > and enzyme histochemistry have been added to this edition to help > histotechnologists preparing for the HTL(ASCP) certifying examination. > Each > unit presents the learning objectives of the section, explains and > illustrates the technique, and then gives specific learning activities > that > will reinforce the information. Written as both a teaching tool and > reference, the format also makes troubleshooting fast and easy. > > Histotechnology is an excellent learning tool for histologic technic > students, and for residents and pathologists seeking to expand their > understanding of the technology used in the histopathology laboratory. > It is > equally well suited as a desk reference for the more common special > staining > techniques and their results. > > Partial Contents > Fixation; Processing; Instrumentation; Safety; Laboratory Mathematics > and > Solution Preparation; Nuclear and Cytoplasmic Staining; Carbohydrates > and > Amyloid; Connective and Muscle Tissue; Nerve; Microorganisms; Pigments, > Minerals, and Cytoplasmic Granules; Immunohistochemistry; Enzyme > Histochemistry; Electron Microscopy. > > Hardbound; 312 pages; 286 images; 6 tables; 47 figures; 1997 > ISBN: 089189411X > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > ----- Original Message ----- > From: > To: > Sent: Saturday, December 27, 2003 8:19 AM > Subject: [Histonet] buying books > > >> I recently have tried purchasing histotechnology examinations: the >> board > of >> registry study guide from amazon....i put my order in november 9th and > have not >> received the book. Does anyone know of somewhere else i could try and >> find >> this excellent book. thanks Jennell >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > ------------------------------ > > Message: 2 > Date: Mon, 29 Dec 2003 20:51:01 GMT > From: mprice26@juno.com > Subject: [Histonet] Minnesota Method for staining Bone Marrows' > To: histonet@lists.utsouthwestern.edu > Message-ID: <20031229.125149.25056.1398144@webmail20.nyc.untd.com> > Content-Type: text/plain > > > Hi Histonetters, > Hope everyone had a Merry Christmas and will have a Happy New Year. > > I need a copy of the Minnesota Method of staining Bone Marrows'. Would > anyone happen to have one that they could e-mail to me or fax to me? > > Thank you. > > Marsha Price > > ________________________________________________________________ > The best thing to hit the internet in years - Juno SpeedBand! > Surf the web up to FIVE TIMES FASTER! > Only $14.95/ month - visit www.juno.com to sign up today! > > > > ------------------------------ > > Message: 3 > Date: Mon, 29 Dec 2003 15:45:30 -0700 > From: "Patsy Ruegg" > Subject: [Histonet] influenza A > To: "Histonet@Pathology. Swmed. Edu" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Looking for a lab that tests for "influenza A" in a paraffin tissue > block, > does that test exist? > Thanks and Happy New Year! > Patsy Ruegg > > > > > ------------------------------ > > Message: 4 > Date: Mon, 29 Dec 2003 18:13:30 -0500 > From: "Linda McDonald" > Subject: [Histonet] CPT Code > To: histonet@lists.utsouthwestern.edu > Cc: Gwen Goss > Message-ID: > Content-Type: text/plain; charset=us-ascii > > When doing Oil Red O stains, Gomori's Trichrome on Frzen muscle tissue > or Pas on frozen muscle tissue, what CPT codes are folks using for > this? Are they counted as an 88313 or would they be an 88314? Let me > know what you all are doing with these. Thanks a lot and Happy New > Year!!!!! > LGMc > > > > > > ------------------------------ > > Message: 5 > Date: Mon, 29 Dec 2003 17:37:54 -0600 > From: David Anthony Wright > Subject: [Histonet] re: IHC background [Peng] - rat/Ms X-Rxn? > To: histonet@lists.utsouthwestern.edu > Message-ID: <1072741074.3ff0bad2a07e3@webmail.uchicago.edu> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Baowei Peng (& Histonet) > > Amos Brooks has suggested the problem below might be endogenous > biotin, with > helpful suggestions what to do. I'd like to suggest something else if > that > doesn't work. A couple of questions- > 1) Is your anti-mouse secondary a polyclonal serum (almost certainly) > and, if > so, is it not rat-adsorbed? > 2) Is there any reason for there being an inflammatory reaction in > your tissue? > > There is about a 5-10% overlap in crossreactivity between anti-mouse > and anti- > rat IgG antibodies (cf. goat & sheep), depending on how distant the > host > species is. I've used standard Vector biotinylated anti-mouse IgG > secondary > (BA2000) happily for IHC on rat brain for years with no background but > got a > huge background cross reaction in every animal when staining material > 2 days > after brain surgery when inflammation is extensive. I know it was > X-reaction > with native rat IgGs in the tissue because the problem vanished when I > either > i) added normal rat serum to the 2ndary incubation mix and so competed > out the > cross reacting moiety or ii) used Vector's rat-adsorbed secondary > (BA2001). > It's more expensive of course! > > good luck! > -David Wright >> Hi,Histoneters >> I'm doing IHC on rat skeletal muscle. I got a heavy background stain >> on >> all sections and control section without 1st AB. But there is no >> stain on >> the control section without 2nd Ab. >> It looks like the 2nd Ab will react with the rat tissue. My first Ab >> is >> derived from Mouse, and second Ab is anti-mouse. >> What could I do to reduce the background stain?-Baowei Peng > -- > David A. Wright, Ph.D. > University of Chicago > Section of Neurosurgery > ================================================ > Does 2+2=5 for large values of 2? [YES!] > > > > ------------------------------ > > Message: 6 > Date: Tue, 30 Dec 2003 9:52:48 +0800 > From: "Baowei Peng" > Subject: [Histonet] How to educe back ground > To: Histonet@lists.utsouthwestern.edu > > Message-ID: <2S968769905369.06076@send6.inner-21cn.com> > Content-Type: text/plain; charset="GB2312" > > Hi,all > Thanks for your kindly responses. > My second Ab is said to be a mAb in the description file from > manufacture. But do not say if it was absorbed by rat IgG, problely > not. > David Wright is right. There is an inflammatory reaction as seen by > mononuclear cell infiltration and diapedesis in the tissue. > So maybe add rat serum to blocking and antibody diluent reagent will > work. I will try this out. > > > > ????????????????Baowei Peng > ????????????????pengbaowei@21cn.com > ????????????????????2003-12-30 > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 30 Dec 2003 06:00:50 -0800 > From: "Tommy Vedilago" > Subject: [Histonet] Jobs in Histology > To: "Histonet (E-mail)" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hello again Histonetters, > We are still looking for highly qualified and energetic managers for > several locations across the country. We need supervisors in > Washington, D.C., Philadelphia, Maine, Houston, NYC, Tucson, AZ, > Tampa, New Jersey, Nashville, and Brooklyn. We are also looking for > techs in various locations and we are now accepting travelers who are > tired of dealing with the large companies. Please feel free to contact > me via email or at 866-797-8361. I hope all of you had a merry > Christmas and have a happy New Year. > > Tommy Vedilago > System 1 Search > (864) 627-0012 > (864) 627-0013 Fax > > > > ------------------------------ > > Message: 8 > Date: Tue, 30 Dec 2003 11:41:26 -0600 > From: "Molinari, Betsy" > Subject: [Histonet] COBr2 > To: > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hi histonetters, > A researcher has given us a protocol he found from quite a while ago > and it calls for a chemical COBr2. The chemical name is carbonyl > bromide. We cannot find a supplier. Does anyone know about this > chemical? > Thanks, > Betsy Molinari HT (ASCP) > Texas Heart Institute > Houston, TX 77225 > 832-355-6524 > 832-355-6812 (fax) > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 1, Issue 191 > **************************************** > From LuckG <@t> empirehealth.org Tue Dec 30 13:53:54 2003 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Polyclonal "Desmin" Message-ID: Hello, One of my pathologists is asking for a polyclonal Desmin antibody. I have found one but it is Rabbit Anti-Chicken is only applicable on frozen sections. I'm looking for a Rabbit Anti-human antibody that will work on FFPE tissues. Thanks Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 509.473.7077 luckg@empirehealth.org From mcauliff <@t> umdnj.edu Tue Dec 30 13:56:53 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] COBr2 In-Reply-To: References: Message-ID: <3FF1D885.3020106@umdnj.edu> Type "carbonyl bromide" into google and see the results. Seems it is an ingredient in fire extinguishers. Geoff Molinari, Betsy wrote: >Hi histonetters, > A researcher has given us a protocol he found from quite a while ago >and it calls for a chemical COBr2. The chemical name is carbonyl >bromide. We cannot find a supplier. Does anyone know about this >chemical? >Thanks, >Betsy Molinari HT (ASCP) >Texas Heart Institute >Houston, TX 77225 >832-355-6524 >832-355-6812 (fax) > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From juan.gutierrez <@t> christushealth.org Tue Dec 30 14:11:49 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Polyclonal "Desmin" Message-ID: Try Lab Vision. They have a rabitt anti-human desmin, polyclonal Ab. The cat# is RB-9014. It comes in purified or ready-to-use forms. I have not used it, but they claim it works on FFPE tissues. Their web address is www.labvision.com and the toll free # is 1-800-828-1628. Good luck. Juan -----Original Message----- From: Luck, Greg D. [mailto:LuckG@empirehealth.org] Sent: Tue 12/30/2003 1:53 PM To: 'HISTONET@LISTS.UTSOUTHWESTERN.EDU' Cc: Subject: [Histonet] Polyclonal "Desmin" Hello, One of my pathologists is asking for a polyclonal Desmin antibody. I have found one but it is Rabbit Anti-Chicken is only applicable on frozen sections. I'm looking for a Rabbit Anti-human antibody that will work on FFPE tissues. Thanks Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 509.473.7077 luckg@empirehealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BRIAN.CHELACK <@t> usask.ca Tue Dec 30 14:31:10 2003 From: BRIAN.CHELACK <@t> usask.ca (Brian Chelack) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Re: Influenza A Message-ID: <3FF1E08E.8061AAB0@sask.usask.ca> Hi Patsy; We have immunostained for Influenza A in porcine, equine, and have had some success with avian paraffin blocks. Our monoclonal antibody is purported to react with >50 isolates from H1 through H14. We've never stained for Influnza A on a human case, but only because we've never been asked. regards Brian Chelack Prairie Diagnostic Services Saskatoon, SK From asmith <@t> mail.barry.edu Tue Dec 30 14:32:27 2003 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] COBr2 Message-ID: <494304423C63E246A5CF87A3AEEB577011B5BE@bumail01.barrynet.barry.edu> Carbonyl bromide is not [repeat NOT] uses as an ingredient in fire extinguishers! Carbonyl bromide is an extremely toxic, low boiling liquid (boils at 140 degrees F). Some fire extinguishers contain Halon 1301 or Halon 1211 which can be converted to carbonyl bromide by very hot fires (temperatures over 1000 degrees F). This restricts their use to very small fires. Since carbonyl bromide is currently classified as a "war gas", it may be hard to buy. (Carbonyl bromide is chemically and toxicologically similar to phosgene, which the British used to great effect in WWI.) Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Tuesday, December 30, 2003 2:57 PM To: Molinari, Betsy Cc: histonet@pathology.swmed.edu Subject: Re: [Histonet] COBr2 Type "carbonyl bromide" into google and see the results. Seems it is an ingredient in fire extinguishers. Geoff Molinari, Betsy wrote: >Hi histonetters, > A researcher has given us a protocol he found from quite a while ago >and it calls for a chemical COBr2. The chemical name is carbonyl >bromide. We cannot find a supplier. Does anyone know about this >chemical? Thanks, >Betsy Molinari HT (ASCP) >Texas Heart Institute >Houston, TX 77225 >832-355-6524 >832-355-6812 (fax) > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From fmonson <@t> wcupa.edu Tue Dec 30 14:50:19 2003 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] COBr2 Message-ID: To get information on chemicals such as "Carbonic dihydride" you should become familiar with some, if not all, of the chemical safety databases that exist online. One of the best resources is at NIST. Use the CAS Number = 593-95-3 and this URL: http://chem.sis.nlm.nih.gov/chemidplus/cmplxqry.html Then choose the NIST WebBook from the frame on the left. BTW, the stuff is a corrosive byproduct of Halon 1211 (as well as other substances) when "1211" is used in fire fighting. I'd love to hear its use in histochemistry. There are some things for which a large BIG BROTHER is most useful. Cheers, Fred Monson West Chester University -----Original Message----- From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu] Sent: Tue 12/30/2003 12:41 PM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] COBr2 Hi histonetters, A researcher has given us a protocol he found from quite a while ago and it calls for a chemical COBr2. The chemical name is carbonyl bromide. We cannot find a supplier. Does anyone know about this chemical? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Houston, TX 77225 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rebecca.riesen <@t> dsilabs.com Tue Dec 30 15:53:32 2003 From: rebecca.riesen <@t> dsilabs.com (Riesen, Rebecca) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] tissue tek cryostat 4553 Message-ID: <05844092236E7749A1BBBCB1077C34A209DD3B@dsi-ex01.gateway.dom> I've got one for you all! We had a fire marshal come through the other day. We have a very small room in one of our satellite facilities where we do frozen sections. When he opened the door, he smelled xylene and alcohol fumes (imagine that!) He then proceeded to ask if this machine ( the tissue tek cryostat 4553) was made to be safely operated in an "explosive environment"? Well, I said I was sure it was ok for the level of fumes in the room (which has been tested to be within safety limits). He wants DOCUMENTATION. I called Sakura Finetek who says that they do not support this piece of equipment and Bayer had until 2000. But as far they know, no one is around to help out with real technical questions on these cryostats. The fire marshal wants something in writing that says something to the effect of.....Store flammable or explosive materials X feet away from this equipment. Or something of that nature. Help me if you can.. someone out there in histo land. THANKS!!!! From nklemme <@t> corp.sakuraus.com Tue Dec 30 18:20:21 2003 From: nklemme <@t> corp.sakuraus.com (Nancy Klemme) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] tissue tek cryostat 4553 In-Reply-To: <05844092236E7749A1BBBCB1077C34A209DD3B@dsi-ex01.gateway.dom> Message-ID: <003301c3cf33$e15dabc0$3b04100a@NancyK> Dear Rebecca, It is highly unlikely that any cryostats manufactured today (much less those manufactured 10 or more years ago) will be rated (and labeled) for use in an explosive environment. That is a rating that can be found on freezers and refrigerators that are specially designed for "storage of volatile materials or for use in fire/explosive hazardous areas." And those products will be clearly labeled for any user or inspection agent to see. I am sure that when Bayer manufactured the Tissue-Tek Cryostats no considerations were made for operating the unit in a chemically laden environment. There are several testing agencies that place their qualifying label of approval on the Sakura cryostat that is currently being manufactured. None are for an explosion proof rating. I'm sorry that this may not be the answer you were seeking. But I do send kind regards and wishes for a happy 2004. Nancy Klemme Mgr, Technical Support Sakura Finetek USA, Inc. 800-725-8723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Tuesday, December 30, 2003 1:54 PM To: histonet@pathology.swmed.edu Subject: [Histonet] tissue tek cryostat 4553 I've got one for you all! We had a fire marshal come through the other day. We have a very small room in one of our satellite facilities where we do frozen sections. When he opened the door, he smelled xylene and alcohol fumes (imagine that!) He then proceeded to ask if this machine ( the tissue tek cryostat 4553) was made to be safely operated in an "explosive environment"? Well, I said I was sure it was ok for the level of fumes in the room (which has been tested to be within safety limits). He wants DOCUMENTATION. I called Sakura Finetek who says that they do not support this piece of equipment and Bayer had until 2000. But as far they know, no one is around to help out with real technical questions on these cryostats. The fire marshal wants something in writing that says something to the effect of.....Store flammable or explosive materials X feet away from this equipment. Or something of that nature. Help me if you can.. someone out there in histo land. THANKS!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- [This E-mail scanned for viruses by Declude Virus] --- [This E-mail scanned for viruses by Declude Virus] From BMolinari <@t> heart.thi.tmc.edu Wed Dec 31 06:44:19 2003 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] COBr2 Message-ID: Thank you to all who answered my question. I did go to MSDS websites, but there was much there. Thanks to Mr. Monson, I did go to the NIST WebBook and found the most information and it is, as Mr. Monson and Dr. Smith pointed out, a by-product of Halon 1211. After presenting all this to the fellow he has changed his mind and decides that it is CoBr2 (cobalt bromine). I think we will send him back to the library. Frustrating. Thanks again. Happy New Year! Betsy Molinari Texas Heart Institute Houston, TX 77225 832-355-6524 832-355-6812 (fax) From BMolinari <@t> heart.thi.tmc.edu Wed Dec 31 09:18:47 2003 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] COBr2 Message-ID: Noel, Sorry for the confusion, initially I was talking about COBr2, then the researcher came back and stated that it may not be COBr2 but CoBr2. Yes, COBr2 is the byproduct of Halon 1211. Betsy -----Original Message----- From: Noel D. Clark [mailto:Noel.Clark@ORTHO.UAB.EDU] Sent: Wednesday, December 31, 2003 7:08 AM To: Molinari, Betsy Subject: RE: [Histonet] COBr2 I'm slightly confused about your email to the histonet. Initially, you wrote COBr2 (carbonyl bromide) and then in your reply you wrote CoBr2 (cobalt bromine). Yet your email sounds like cobalt bromine is the byproduct of Halon 1211. COBr2 (carbonyl bromide) is the byproduct of Halon, not cobalt bromide. Curious, noel -----Original Message----- From: Molinari, Betsy [mailto:BMolinari@heart.thi.tmc.edu] Sent: Wednesday, December 31, 2003 6:44 AM To: histonet@pathology.swmed.edu Subject: [Histonet] COBr2 Thank you to all who answered my question. I did go to MSDS websites, but there was much there. Thanks to Mr. Monson, I did go to the NIST WebBook and found the most information and it is, as Mr. Monson and Dr. Smith pointed out, a by-product of Halon 1211. After presenting all this to the fellow he has changed his mind and decides that it is CoBr2 (cobalt bromine). I think we will send him back to the library. Frustrating. Thanks again. Happy New Year! Betsy Molinari Texas Heart Institute Houston, TX 77225 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed Dec 31 10:18:30 2003 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] CPT Code Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB3F29@fh2k093.fhmis.net> We are using CPT code# 88314 for the stains you list and 88319 for the enzyme stains. -----Original Message----- From: Linda McDonald [mailto:mcdonal1@ccf.org] Sent: Monday, December 29, 2003 6:14 PM To: histonet@lists.utsouthwestern.edu Cc: Gwen Goss Subject: [Histonet] CPT Code When doing Oil Red O stains, Gomori's Trichrome on Frzen muscle tissue or Pas on frozen muscle tissue, what CPT codes are folks using for this? Are they counted as an 88313 or would they be an 88314? Let me know what you all are doing with these. Thanks a lot and Happy New Year!!!!! LGMc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From georgecole <@t> ev1.net Wed Dec 31 11:27:02 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] MSDS WEBSITES AND NIST OR HIST WEB BOOK Message-ID: <000001c3cfc3$4e8ece60$064dbad0@hppav> Histotechs; I saw the MSDS WEBSITES and the NIST, or, HIST WEB BOOK mentioned on the Histonet this morning. Are they websites similar to the Histonet? If so, how do you call them up?----I for one am interested in all the information I can get!! georgecole@ev1.net From tpmorken <@t> labvision.com Wed Dec 31 13:28:13 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] anti-mouse antibody info site Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CE89@usca0082k03.rallansci.apogent.com> Here is a good site for information about antibodies to be used on mouse tissue: NCI-Frederick, Laboratory Animal Sciences Program http://web.ncifcrf.gov/rtp/lasp/phl/immuno/ Tim Morken Lab Vision / NeoMarkers email: tpmorken@labvision.com www.labvision.com From sharon.willman <@t> bms.com Wed Dec 31 14:22:12 2003 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Fri Sep 16 15:22:23 2005 Subject: [Histonet] Histonet Caspase-3 and Mast Cells Message-ID: <3FF32FF4.2F22FED8@bms.com> Hi, I was wondering if Caspase 3 stains mast cells. If anyone has any information on this I would appreciate any help given. Thanks, Sharon Willman