[Histonet] Mast vs eosinophils

[rtarmen <@t> drizzle.com]

Hi everyone,

I need input on determining between mast and eosinophils. I am staining
formalin fixed paraffin embedded human scar tissue with acidified
tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells
that I believe are eosinophils, and I only want mast cells with intact
granulation.

Any suggestions?

_cheers,

Rebecca Armendariz
Harborview Medical Center
Univerisity of Washington, Seattle
rtarmen <@t> drizzle.com