[Histonet] Re: processing polymers

[Chan Wai Kam]

Hi Linda,

I'm from the experimental surgery unit of the dept. of Orthopaedic
Surgery and some of our projects right now use scaffolds(poly
carbolactone, poly-lactic and poly-glycolic) for the study of tissue
repair.  Our usual processing and paraffin embedding procedures
dissolves away the scaffold.  Would you know of any procedures for
paraffin sections we could use that would leave the scaffold intact so
that it can be viewed in slides.

Thanks
Julee Chan 
National University of Singapore
   

-----Original Message-----
From: Linda Jenkins [mailto:jlinda <@t> ces.clemson.edu] 
Sent: Thursday, August 28, 2003 2:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: processing polymers


Ian,
         You wrote:

         "Does anybody have any helpful hints for processing and
paraffin embedding polymers such as poly caprolactone, polyurethanes,
polylactide etc. Thanks, Ian Birchall, Melbourne."

         We do lots of embedding of polymers and have found it depends 
entirely on the polymer.  Some are light, heat, and chemically 
reactive.  Then you have to play "Merlin-the-Magician"!  Seriously, are
you 
sectioning beads, scaffolds or gels?  I have a PowerPoint handout I can 
send you if you would be interested?
         Linda

Linda Jenkins, HT
Clemson University
Dept. of Bioengineering
Clemson, SC 29634-0905
864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm


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