[Histonet] RE: frozen tissue

[C.M. van der Loos]

Hi Martha,
How about embedding the tissue in paraffin as usual and perform 
Immunogold-silver staining? The accuracy is as good as fluorescence and 
you also have the option of "epipolarization microscopy" using an 
epipolarization (or IGS block) in your fluorescence scope. The result 
certainly competes with immunofluorescence. Please have a look at 
www.aurion.nl for info about this rather unknown but very elegant IHC 
technique.

Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands

>From: Martha Ward [mailto:mward <@t> wfubmc.edu] 
>Sent: Fri 8/22/2003 7:58 AM 
>To: histonet <@t> pathology.swmed.edu 
>Cc: 
>Subject: [Histonet] frozen tissue
>
>I have a situation that I hope I can get some help with.  My 
>neuropahtologist had some nerve tissue in formalin for several weeks.  
>He gave the tissue to a resident, had him bisect the tissue and asked 
>him to order immunofluorescent studies (IgG, IgM).  He told the 
>resident to wash the tissue in water and OCT embedding media, freeze 
>and cut sections.  Has anyone ever tried this and if so how did things 
>turn out?  Thanks in advance for the help. 
>
>Martha Ward
>Wake Forest University Baptist Medical Center