[Histonet] frozen tissue

[Morken, Tim - Labvision]

The problems with using formalin-fixed tissue for frozen/fluorescent studies
is that 1) formalin-fixed tissue has high autofluoresence, and 2)
formalin-fixed frozen tissue does not stick to slides very well. It may be
that you can get acceptiable IgG and IgM staining with normal IHC
procedures. I've done that on kidney tissue in which the frozen material was
inadequate. The formalin tissue showed good staining and was acceptable for
diagnosis.

Tim Morken
Lab Vision / NeoMarkers



-----Original Message-----
From: Martha Ward [mailto:mward <@t> wfubmc.edu] 
Sent: Friday, August 22, 2003 5:58 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] frozen tissue

I have a situation that I hope I can get some help with.  My
neuropahtologist had some nerve tissue in formalin for several weeks.  He
gave the tissue to a resident, had him bisect the tissue and asked him to
order immunofluorescent studies (IgG, IgM).  He told the resident to wash
the tissue in water and OCT embedding media, freeze and cut sections.  Has
anyone ever tried this and if so how did things turn out?  Thanks in advance
for the help.  

Martha Ward
Wake Forest University Baptist Medical Center

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