[Histonet] cryostat decontamination

[Bill Sinai]

Laura,

We have a protocol for decontaminating all our Cryostats once per week.  We
have 2 Shandon 660E models which have the heating block which you fill with
40% formadlehyde then turn on the decontamination cycle.  This is performed
overnight Friday.  After this process the shavings are removed using tissue
damped with 70% ethanol.
The others have a formaldehyde "bomb" prepared and placed into the chamber,
then left sealed to defrost for 24 hours.  These are then cleaned using 70%
ethanol.

The same procedures are carried out if we have cut anything suspected of
being an infective agent, immediately after the case is finished.

We have the luxury of two cryostats available in our main laboratory.

All the best
Bill Sinai
Laboratory Manager
Tissue Pathology
ICPMR
P.O. Box 533
Wentworthville NSW 2145
pH 02 9845 7774
----- Original Message -----
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 21, 2003 5:03 AM
Subject: [Histonet] cryostat decontamination


How do others clean the cryostat after a known or suspected TB case?  Also,
do you decontaminate once a week with a tuberculocidal antiseptic?

Laurie Colbert
Huntington Memorial Hospital

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