[Histonet] 0.5 um MMA sections

[Gayle Callis]

If you are cutting this thin, the knife must be extremely sharp.  Neil Hand
cut 1 um PMMA aka MMA with a triangular glass knife and probably NOT a
large faced block.  It was more like sectioning for EM. You might get by
with a very sharp TC knife, otherwise a better knife edge may be necessary.  

If you are trying to do immunostaining, the MMA would have to be removed as
it is so hydrophobic, permeabilization methods will not work.  Removal of
MMA is easy compared to other plastics. 

What is the purpose of having sections this thin in the first place? 

 


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis <@t> montana.edu