[Histonet] GMS Precipitant

[Jeannie Heck]

The procedure that we use for our Gomori Methenamine Silver (GMS) stain comes directly from Frieda Carson's Histotechnology: A Self Instructional Text. The microwave procedure has worked well for us for many years but about 6 months ago we started getting a dark black precipitant on our slides and the inside of our Coplin jars. We have tried numerous different potential solutions, such as chemically cleaning our Coplin jars, using various types of deionized water (all Type 1), preparing fresh solutions and adjusting the temperature by decreasing and increasing the heating time. When we first encountered this problem it did not occur with the conventional method, but lately it has been occuring with both methods. Any help will be appreciated. Thank you.
 
Jeannie Heck HT (ASCP)


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