[Histonet] permeabilization for nuclear staining?

[jason madore]

I have a question regarding using detergents to permeabilize tissue for 
nuclear staining.
I suppose that fixing 5 minutes in acetone:EtOH (3:1) will allow access to 
the cytoplasm, but what about if you are also looking for rare nuclear 
staining (NF-KB)? I am thinking that the acetone will also open the nucleus 
but not sure if using a detergent may also be prudent? My guess is probably 
not but if anyone else has thoughts on this I would like to hear them.

ciao,

J,

Jason Madore
Institut du cancer de Montréal
Centre de recherché du CHUM
1560 Rue Sherbrooke est
Room Y-4609
Montréal, QC H2L 4M1 Canada

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