From STapper <@t> slhduluth.com Thu Aug 7 09:33:34 2003 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <3BFBBD68413CB443A7125A63EC0ACD1D33A48B@slhw2smail01.slhdomain.com> Sheila Tapper -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030807/20bfabdf/attachment.htm From jason_abrams <@t> compbio.com Mon Aug 11 10:18:51 2003 From: jason_abrams <@t> compbio.com (Jason Abrams) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Paigen gelatin procedure Message-ID: I need help with tissue being fragmented in bloc of paraffin, tissue that is in gelatin, will not cut properly thank you Nancy Pinard From jason_abrams <@t> compbio.com Mon Aug 11 10:19:36 2003 From: jason_abrams <@t> compbio.com (Jason Abrams) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Paigen gelatin procedure Message-ID: I need help with fragmented tissue when cutting in paraffin bloc tissue piece is all fragmented. can I have a procedure to compare to mine nancy_pinard@hotmail.com From hkhagler <@t> mac.com Thu Aug 14 10:18:47 2003 From: hkhagler <@t> mac.com (Herb Hagler) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] a test message Message-ID: <99C3577E-CE6A-11D7-B897-000A958F77CC@mac.com> This is a test message From juan.gutierrez <@t> christushealth.org Thu Aug 14 11:22:19 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] a test message Message-ID: Just how many histonets are there? I was subscribed to one that had about 40 messages per day, now I only get one every other week. Any comments? Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Health Care 333 N. Santa Rosa Ave. San Antonio, TX 78207 (210)704-2533 -----Original Message----- From: Herb Hagler [mailto:hkhagler@mac.com] Sent: Thu 8/14/2003 10:18 AM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] a test message This is a test message _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hagler.herb <@t> pathology.swmed.edu Sat Aug 16 12:15:29 2003 From: hagler.herb <@t> pathology.swmed.edu (Herb Hagler) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Welcome to the new list server from Herb Message-ID: <3C54E523-D00D-11D7-B4FA-000A958F77CC@pathology.swmed.edu> I hope everyone reads the message and understands it. Those that were getting the digest mode will have to log on to the website in the message, use your password which was included at the bottom of the message and chose the digest mode again. Good Luck and thanks for your patience with us. Herb From masonb <@t> ohsu.edu Sat Aug 16 14:29:07 2003 From: masonb <@t> ohsu.edu (Barbra Mason) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Histonet mailing list sign up Message-ID: Thank you masonb@ohsu.edu From hylanderl <@t> comcast.net Sat Aug 16 14:45:06 2003 From: hylanderl <@t> comcast.net (Linda Hylander) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe to digest Message-ID: <001201c3642e$e535fe20$6401a8c0@ne2.client2.attbi.com> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030816/7ad50b61/attachment.htm From vbaker60 <@t> yahoo.com Sat Aug 16 16:42:58 2003 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe Histonet Message-ID: <20030816214258.58292.qmail@web12104.mail.yahoo.com> vbaker60@yahoo.com __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software http://sitebuilder.yahoo.com From badesuyi <@t> hotmail.com Sat Aug 16 18:31:49 2003 From: badesuyi <@t> hotmail.com (Banjo Adesuyi) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] histonet mailing list signup Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030817/fd2374c6/attachment.htm From DMBCMP <@t> aol.com Sat Aug 16 18:38:56 2003 From: DMBCMP <@t> aol.com (DMBCMP@aol.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Subscribe Histonet Message-ID: <18c.1e82dd5e.2c701a90@aol.com> Thank you. Dannie From JMeade0710 <@t> aol.com Sat Aug 16 22:15:44 2003 From: JMeade0710 <@t> aol.com (JMeade0710@aol.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] log-on Message-ID: <11a.2714e09b.2c704d60@aol.com> Thanks Herb for all your hard work. JMeade0710@aol.com Jerry Meade -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030816/684c2a0f/attachment.htm From lbeitman63 <@t> sbcglobal.net Sat Aug 16 23:34:49 2003 From: lbeitman63 <@t> sbcglobal.net (Elizabeth Beitman) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (no subject) Message-ID: <20030817043449.99491.qmail@web80305.mail.yahoo.com> lbeitman63@sbcglobal.net From g-gduncan <@t> msn.com Sun Aug 17 05:27:15 2003 From: g-gduncan <@t> msn.com (Gary & Gina Duncan) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: From g-gduncan <@t> msn.com Sun Aug 17 05:32:12 2003 From: g-gduncan <@t> msn.com (Gary & Gina Duncan) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: From dmccaig <@t> ckha.on.ca Sun Aug 17 09:14:12 2003 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (no subject) Message-ID: Diana McCaig, R.T. Charge Tech, Histology Chatham Kent Health Alliance 519-352-6401 (3347) From koellinr <@t> amgen.com Sun Aug 17 09:27:04 2003 From: koellinr <@t> amgen.com (Koelling, Raymond) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Test Message-ID: Test Ray Koelling Amgen Corp. Seattle, WA From KMB1904 <@t> aol.com Sun Aug 17 09:32:18 2003 From: KMB1904 <@t> aol.com (KMB1904@aol.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] histonet Message-ID: <4c.20f01a56.2c70ebf2@aol.com> Just a test!! Kathy Bowden HT Nanticoke Memorial Hospital Seaford, De 19973 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030817/74d42d2f/attachment.htm From TomBuck <@t> metc.net Sun Aug 17 10:37:53 2003 From: TomBuck <@t> metc.net (Hawkeye Biomedical, LLC Tom Buck) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: <002c01c364d5$85e5afe0$6601a8c0@tom> Thanks for letting us subscribe! Tom Buck Member Manager Hawkeye Biomedical, LLC PO Box 165 Atlantic, IA 50022 877-423-9879 fax 712-243-4501 E-mail TomBuck@metc.net www.hawkeyebiomedical.com We are a histology equipment service vendor. From Dndsomi <@t> aol.com Sun Aug 17 10:37:57 2003 From: Dndsomi <@t> aol.com (Dndsomi@aol.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Test Message-ID: <12c.300f406d.2c70fb55@aol.com> D Dietz Morristown-Hamblen Healthcare System Morristown, TN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030817/83b6bc58/attachment.htm From Twmshisto <@t> wmconnect.com Sun Aug 17 10:49:38 2003 From: Twmshisto <@t> wmconnect.com (Twmshisto@wmconnect.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (no subject) Message-ID: <127.2f469884.2c70fe12@wmconnect.com> thanks -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030817/f12aa0b8/attachment.htm From georgecole <@t> ev1.net Sun Aug 17 13:26:36 2003 From: georgecole <@t> ev1.net (George Cole) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] all those muscle and nerve procedures packets Message-ID: <000001c364ed$18d73690$034dbad0@hppav> Hey out there! What's doing on the muscle and nerve front? I would be happy to hear from you folks out there that got the DVD's and pages. A wish from this old tech out to pasture---- I hope they are a help to you techs doing muscle and nerve biopsies. Let me a just drop a strong paragraph, then run----each and every one of those techniques contained in the packet, when I somehow found them as a new and better twist to a biopsy work move was usually awkward at first----the old inadequate move easier to do, more familiar. But practice can smooth new moves into practical do-able helps to application.----There's really is no defense for ignoring quality updates. The patient will be out there thinking he sent a piece of his body to you for your best efforts to find what's wrong with him, and the news he's probably praying for, may be hidden anywhere in that bit of his person. Any and all moves that will help conduct diligent searches for that hidden information should be put to work.----we know hunting through biopsy tissues can never be absolutely complete---but we can sure chase after that 100%. Besides, working on automatic pilot is a big fat bore!!! Dang! I know I should avoid soap boxes for smooth diplomatic cajoling----but FOOP!!! DA DAT TA DUUUHH C H A R G E !!! . -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030817/d9636d5d/attachment.htm From jluis.palazon <@t> icman.csic.es Sun Aug 17 13:39:14 2003 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] testing new server Message-ID: <20030817183914.3E2731E8E1@perceval.uca.es> testing the new address of the net From escott8 <@t> houston.rr.com Sun Aug 17 14:03:51 2003 From: escott8 <@t> houston.rr.com (Edward Scott) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Subscribe digest Message-ID: <000001c364f2$4c496ed0$cf03a718@thescotts> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030817/e9005dc1/attachment.htm From g-gduncan <@t> msn.com Sun Aug 17 14:29:40 2003 From: g-gduncan <@t> msn.com (Gary & Gina Duncan) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (no subject) Message-ID: test From KHays <@t> mbhs.org Sun Aug 17 17:29:30 2003 From: KHays <@t> mbhs.org (KHays@mbhs.org) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: Kathy Tedford-Hays HT (ASCP) Technical Specialist, Histology Dept (601)-968-3070 ext 7398 Baptist Medical Center 1225 North State Street Jackson, MS 39202 ----- Forwarded by Kathy Hays/BHS on 08/17/03 05:28 PM ----- "Hawkeye Biomedical, LLC Tom Buck" To: Sent by: cc: histonet-admin@lists.utsouth Fax to: western.edu Subject: [Histonet] subscribe digest 08/17/03 10:37 AM Thanks for letting us subscribe! Tom Buck Member Manager Hawkeye Biomedical, LLC PO Box 165 Atlantic, IA 50022 877-423-9879 fax 712-243-4501 E-mail TomBuck@metc.net www.hawkeyebiomedical.com We are a histology equipment service vendor. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this email is confidential and is intended for the recipient only. Do not forward this email without permission from its originator. From doscwk <@t> nus.edu.sg Sun Aug 17 19:59:39 2003 From: doscwk <@t> nus.edu.sg (Chan Wai Kam) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (Histonet) testing Message-ID: Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From willthon <@t> msn.com Sun Aug 17 21:16:03 2003 From: willthon <@t> msn.com (William Thoendel) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: _________________________________________________________________ Add photos to your e-mail with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=features/featuredemail From swong <@t> rigel.com Sun Aug 17 23:39:22 2003 From: swong <@t> rigel.com (Steve Wong) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] testing new server References: <20030817183914.3E2731E8E1@perceval.uca.es> Message-ID: <014c01c36542$b28148d0$1c02000a@SWONGTP> One more try... test? Please keep me on the list! I've learned a lot! Steve Steve Wong Associate Scientist Rigel Pharmaceuticals, Inc. 1180 Veterans Boulevard South San Francisco, CA 94080 (650)624-1354 office (650)269-1596 cell (650)624-1101 FAX swong@rigel.com ----- Original Message ----- From: "Jose Luis Palazon Fernandez" To: Sent: Sunday, August 17, 2003 11:39 AM Subject: [Histonet] testing new server > testing the new address of the net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hadi83 <@t> comcast.net Mon Aug 18 00:05:13 2003 From: hadi83 <@t> comcast.net (Hadi Yaziji) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <8CDF394F-D139-11D7-B112-00039378E76A@comcast.net> Test.. Hadi Yaziji, M.D. PhenoPath Laboratories From N.Lieuwes <@t> orthop.umcn.nl Mon Aug 18 03:14:38 2003 From: N.Lieuwes <@t> orthop.umcn.nl (Lieuwes, N.) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] FW: unsubscribe Message-ID: <5B31B9F59E138A409E8F7C2183FF36F017B5C9@umcnet13.umcn.nl> -----Oorspronkelijk bericht----- Van: Ramrattan, N. Verzonden: maandag 18 augustus 2003 10:13 Aan: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Onderwerp: unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/065f8549/attachment.htm From N.Lieuwes <@t> orthop.umcn.nl Mon Aug 18 03:14:38 2003 From: N.Lieuwes <@t> orthop.umcn.nl (Lieuwes, N.) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] FW: unsubscribe Message-ID: <5B31B9F59E138A409E8F7C2183FF36F017B5C9@umcnet13.umcn.nl> -----Oorspronkelijk bericht----- Van: Ramrattan, N. Verzonden: maandag 18 augustus 2003 10:13 Aan: histonet@pathology.swmed.edu; histonet@lists.utsouthwestern.edu Onderwerp: unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/065f8549/attachment-0001.htm From Hedley.Glencross <@t> CMMC.nhs.uk Mon Aug 18 03:59:54 2003 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: Cannot access website yet! Hedley Glencross From lpwenk <@t> mail.netquest.com Mon Aug 18 04:16:54 2003 From: lpwenk <@t> mail.netquest.com (Lee & Peggy Wenk) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <003101c36569$77f19b80$8732fea9@hppav> PAW From bruyntjes <@t> voeding.tno.nl Mon Aug 18 04:14:50 2003 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (no subject) Message-ID: <3B070848E7C2204F9DEB8BCFD76772800210D40C@ntexch1.voeding.tno.nl> Testje Joost From lpwenk <@t> mail.netquest.com Mon Aug 18 04:51:07 2003 From: lpwenk <@t> mail.netquest.com (Lee & Peggy Wenk) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Re: Question regarding HT exam References: <2F2517BC.3D356CF6.0004C8D1@aol.com> Message-ID: <004601c3656e$3f5d6880$8732fea9@hppav> No one will be "grandfathered in" to the HT exam, no matter how many years of experience they have, if they are applying through the high school route. The route of high school diploma plus 2 years full time histology experience (on-the-job-training (OJT)) will be discontinued as of Jan. 2005. However, please realize that the candidate has a little bit of additional time after that, IF the candidate's first attempt at taking the exam is before the end of Dec. 2004. Let's assume this scenario - The candidate first takes the exam in the summer of 2004 (July, Aug, Sept) and does not pass. The candidate has a total of 5 times of taking the exam. After 5 attempts, they cannot take the HT exam ever again. (Of course, they could go on and earn a BA or BS, and take the HTL.) Our imaginary person has taken the test once, so has 4 more attempts (5 - 1 = 4). They could try again in the fall 2004 (Oct, Nov, Dec.). If they do not pass, they still have 3 more attempts. These 3 more attempts CAN be taken after the Jan. 2005 deadline, with some restrictions. The other criteria is that, when a person signs up to take the exam, that "exam cycle" is good for five (5) years. Since this candidate signed up to first take the exam in the summer 2004, they have signed up for a cycle that ends spring 2009. So this candidate can take the exam 3 more times, up through spring (Apr, May, June) 2009. If you want to think of it this way, they are "grandfathered in" for being allowed to continue to take the exam. But ONLY if they FIRST signed up and TOOK the exam BEFORE the end of Dec. 2004. The key word is TOOK. If they do not pass by the 5th try, they cannot take the HT exam ever again, no matter what route. In other words, they cannot then earn an associate degree with 20 credit hours of bio/chem, and try taking the HT exam via that route. Now, another scenario. What if this candidate tried twice in 2004 to take the exam, and two more times in the cycle ending spring 2009. They still have 1 more try left (5 - 4 = 1), but their original 5 years is now up. When they go to sign up for the next 5 year cycle, they are NO LONGER be "grandfathered in" for being allowed to take the exam via the high school-OJT route. They are now under a new cycle, and thus are now under the new rules, which will say the candidate must qualify under either the associate degree-OJT route, or the completion of a NAACLS-accredited HT program route. So our imaginary HS-OJT candidate will NOT be allowed to take that last, 5th time, because they don't qualify under the new rules. Last variation. If a person has taken the exam twice in the distant past, such as in 1996 and 1999, did not pass, but today (2003), it is past the 5 year cycle (1996 + 5 years = 2001). They can sign up again for another 5 year cycle (2003 - 2008), but they only have 3 more attempts (5 tries allowed - 2 previous tries taken = 3 attempts left) in the new 5 year cycle. It is 5 attempts total, not 5 attempts each cycle. So, in summary - - Take the exam at least ONCE before the end of Dec. 2004. - If fail, take all 4 additional tries before the end of the 5 year cycle (date starting from when the person first took the HT exam). Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS Program Director, HT and HT Programs William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: To: Sent: Wednesday, August 13, 2003 10:39 AM Subject: Question regarding HT exam > Does anyone know if the High School diploma/2 years full time histology experience route that is needed to take the HT exam will be fully discontinued in January 2005 or will prospective candidates be "grandfathered in?" Thanks in advance for the information. > > Donna Barlow > RCH Labs > Raleigh, NC > From pedro.louro <@t> spcorp.com Mon Aug 18 06:14:09 2003 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <4508920F80C0D411B90200508BF9A9F4030A5083@LAFMSG30.us.schp.com> ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/3a9622b6/attachment.htm From Marjorie.Lehman <@t> unilever.com Mon Aug 18 06:20:55 2003 From: Marjorie.Lehman <@t> unilever.com (marjorie lehman) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Test Message-ID: From antje.marcantonio <@t> pharma.novartis.com Mon Aug 18 07:01:24 2003 From: antje.marcantonio <@t> pharma.novartis.com (antje.marcantonio@pharma.novartis.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/c7753eb2/attachment.htm From wasielewski.reinhard.von <@t> mh-hannover.de Mon Aug 18 07:17:30 2003 From: wasielewski.reinhard.von <@t> mh-hannover.de (wasielewski.reinhard.von@mh-hannover.de) Date: Fri Sep 16 15:21:44 2005 Subject: (Fwd) [Histonet] test Message-ID: <3F40DFFA.13744.16298FA@localhost> HELP ! I am flooded with histonet messages, without any reason. Please stop spaming me with histonet messages ! With best regards Reinhard. ------- Forwarded message follows ------- To: histonet@lists.utsouthwestern.edu From: antje.marcantonio@pharma.novartis.com Date sent: Mon, 18 Aug 2003 14:01:24 +0200 Subject: [Histonet] test ------- End of forwarded message ------- PD Dr. med. Reinhard von Wasielewski From andreah <@t> imclone.com Mon Aug 18 07:18:22 2003 From: andreah <@t> imclone.com (andreah@imclone.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: From JBurrill <@t> criver.com Mon Aug 18 07:24:14 2003 From: JBurrill <@t> criver.com (JBurrill@criver.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: Jason D. Burrill, B.A., HT(ASCP) Senior Supervisor, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 ph: 978-658-6000 ext 1652 fax: 978-988-8793 E-mail: jburrill@criver.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/9f67d64a/attachment.htm From la.sebree <@t> hosp.wisc.edu Mon Aug 18 08:08:58 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From Rita.Humphrey <@t> chsys.org Mon Aug 18 08:24:46 2003 From: Rita.Humphrey <@t> chsys.org (Rita Humphrey) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Alabama Society for Histotechnology Message-ID: Check out our web site Yahoo Alabama Society for Histotechnology From Michele.Ellender <@t> nrpb.org Mon Aug 18 08:26:55 2003 From: Michele.Ellender <@t> nrpb.org (Michele Ellender) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] RE: spam - test messages Message-ID: Can anyone explain why in addition to the daily digests which I do want I am now receiving "test messages", "unsubscribe" and "subscribe" ones as well - 39 messages since the 16th. Just a bit annoying! I'd be grateful if these "extra" messages could stop. Thanks. This e-mail transmission is strictly confidential and intended solely for the person or organisation to whom it is addressed. It may contain privileged and confidential information and if you are not the intended recipient, please do not copy, distribute or take any action in reliance on it. If you have received this e-mail in error, please notify the sender as soon as possible and delete the message. Please note that NRPB monitors incoming and outgoing e-mail for compliance with its Acceptable Use Policy. This will include scanning incoming e-mails to detect viruses and key-words and may in some circumstances result in the manual monitoring of the content of messages. National Radiological Protection Board E-mail: nrpb@nrpb.org Web site: www.nrpb.org -------------------------------------------- From Terry.Marshall <@t> rothgen.nhs.uk Mon Aug 18 08:33:08 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] RE: spam - test messages Message-ID: Yes, I can. It's because one page of explanation and an automatic transfer to the new system is beyond the limited capacity of these people. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk Can anyone explain why in addition to the daily digests which I do want I am now receiving "test messages", "unsubscribe" and "subscribe" ones as well - 39 messages since the 16th. Just a bit annoying! I'd be grateful if these "extra" messages could stop. Thanks. From Hector.Hernandez <@t> tdh.state.tx.us Mon Aug 18 08:36:18 2003 From: Hector.Hernandez <@t> tdh.state.tx.us (Hector Hernandez) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <2F2DADC278A8D511B03E009027991B5F014DE7A6@TDHEXTCID> From CCLYATT <@t> mail.mcg.edu Mon Aug 18 08:38:56 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: From funderwood <@t> mcohio.org Mon Aug 18 08:22:43 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: From gmacke <@t> shrinenet.org Mon Aug 18 08:50:40 2003 From: gmacke <@t> shrinenet.org (Macke, Gail) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From herbert <@t> niehs.nih.gov Mon Aug 18 08:51:05 2003 From: herbert <@t> niehs.nih.gov (Herbert, Ronald (NIH/NIEHS)) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Unsubscribe Message-ID: > Your address has been added to the addresses that comprise this Digest > List. > Welcome to HISTONET. This is an electronic mailing list for the exchange > of > information pertaining to histotechnology and related fields. > > PLEASE SAVE THIS MESSAGE. > It contains useful information about how to use the list and what to do if > you > experience problems. It also includes some basic rules for email etiquette > (Netiquette) which will be helpful to those who are new to this form of > communication. > > WHAT IS A LISTSERVER? > A list server is a computer that runs software which will receive incoming > electronic mail (email) messages and reroute them automatically to > everyone on > the subscriber list. Email uses the vast expanse of the Internet to allow > almost instantaneous communication between networked computers around the > world. Our system uses the LISTSTAR software from Quarterdeck Corporation > (California) and can currently send about 30 messages a minute. With the > present number of subscribers, we are processing about 10,000 outbound > messages a day. > > WHO SHOULD SUBSCRIBE? > Anyone interested in research or clinical applications of histology, > immunohistochemistry, in-situ hybridization pathology, and electron > microscopy > may find Histonet informative and useful. Currently, there are more than > 850 > subscribers from all over the world. Subscribers include hospital > employees > from major urban centers and obscure remote locales, university > researchers, > botanists and the employees of commercial laboratories, government > agencies, > veterinary facilities and a wide variety of commercial industrial > ventures. > > WHO RUNS HISTONET? > The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using > hardware and software owned by the University of Texas Southwestern > Medical > School, Department of Pathology in Dallas, Texas. If you have any > questions or > problems with Histonet please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us. > > HOW DOES THE LIST WORK? > This server, unlike many systems, uses ONLY ONE ADDRESS to send commands > to > the computer and to post messages. The server will recognize commands sent > in > the SUBJECT line of the message and only when they are spelled exactly as > listed below. Anything not identified as a command will be circulated to > EVERYONE on the list. > > The following is a list of commands the server recognizes: > > subscribe > Your address will be added to the list of subscribers. You will then be > able > to send messages to this list that will be forwarded to all other list > subscribers. You will begin to receive all messages sent to the list by > other > subscribers. > > subscribe digest > Your address will be added to the list of subscribers who receive a > digest > instead of each forwarded message. A digest is a compilation of all the > messages received in a 24 hour period. It is sent to the digest > subscribers > every night after midnight. Digest subscribers can post and respond to > messages the same as "real-time" subscribers. > > digests > A list of available digests will be returned to you. Histonet stores old > messages as daily digests for approximately three months. To read > previous > messages, copy the list of available digests, mark the dates of interest > and > return it to the server. > > unsubscribe > Your address will be removed from the list of subscribers. > You will no longer be able to send messages to the members > of the list. > > help > A list of the commands recognized by the server will be returned to > you. > > WHAT ARE THE RULES? > You may post any questions you wish pertaining to histology, pathology, > in-situ hybridization, immunohistochemistry etc. Equipment and reagent > evaluations, laboratory management issues, government regulations, and job > opportunities are all appropriate topics. The University asks that we > restrict > the use of its hardware and software to business purposes only (occasional > jokes do slip through but PLEASE use restraint). Vendors and those with > commercial interests in histology products are welcome contributors > however, > we ask that blatant advertisements be avoided at all times. It is fine to > refer to product that your company produces if it is pertinent to a topic > being discussed on the list. Unsolicited advertisements are poorly > tolerated > by the members and you will likely receive a number of negative comments > if > you overstep the boundaries. Please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us if you are not sure about the > appropriateness > if a message you wish to post. > > > BASIC HISTONET "NETIQUETTE" > It is most helpful to the list members if you post your responses to > queries > to everyone on the list and not just as a personal reply to the person > asking > the question. That way duplicate messages are minimized and we all learn > from > each other's comments. > > Likewise, if you post a question and get a number of responses back > directly > to you, it is helpful to everyone if you could send out a summary of the > replies you got to Histonet. > > Please avoid abbreviations unless they are explained in your message. For > example: immunohistochemistry (IHC). This list circulates to a wide > variety of > individuals and what seems obvious to you may have no meaning on the other > side of the world. > > Please sign your letter and include your institution or affiliation and > location. Not all email systems have headers which identify the sender. > > Do use the subject line to indicate the topic of your message. > > DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. > > Please send questions and problems about the list directly to Linda > Margraf at > LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 > subscribers on the list. Be careful when sending commands to the server to > put > the command in the SUBJECT LINE and spell it correctly. > > Please do not send images as attachments with your message. We can now > post > images at our web site (http://pathcuri1.swmed.edu). To have an image > posted > send it to Herb Hagler at herb.hagler@email.swmed.edu. > > > > > es > > From funderwood <@t> mcohio.org Mon Aug 18 08:53:41 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] puncture resistant gloves Message-ID: Hi all. Is anyone familiar with puncture resistant gloves. Feedback and vendor information would be greatly appreciated. Thank you. Fred Underwood Montgomery County Coroner Dayton, OH From cathy <@t> wasatchhisto.com Mon Aug 18 09:09:27 2003 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: <000a01c36592$57152520$4f693442@selfuggnbwd3wt> subscribe digest -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/84645302/attachment.htm From herbert <@t> niehs.nih.gov Mon Aug 18 09:13:49 2003 From: herbert <@t> niehs.nih.gov (Herbert, Ronald (NIH/NIEHS)) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] UNSUBSCRIBE Message-ID: > Your address has been added to the addresses that comprise this Digest > List. > Welcome to HISTONET. This is an electronic mailing list for the exchange > of > information pertaining to histotechnology and related fields. > > PLEASE SAVE THIS MESSAGE. > It contains useful information about how to use the list and what to do if > you > experience problems. It also includes some basic rules for email etiquette > (Netiquette) which will be helpful to those who are new to this form of > communication. > > WHAT IS A LISTSERVER? > A list server is a computer that runs software which will receive incoming > electronic mail (email) messages and reroute them automatically to > everyone on > the subscriber list. Email uses the vast expanse of the Internet to allow > almost instantaneous communication between networked computers around the > world. Our system uses the LISTSTAR software from Quarterdeck Corporation > (California) and can currently send about 30 messages a minute. With the > present number of subscribers, we are processing about 10,000 outbound > messages a day. > > WHO SHOULD SUBSCRIBE? > Anyone interested in research or clinical applications of histology, > immunohistochemistry, in-situ hybridization pathology, and electron > microscopy > may find Histonet informative and useful. Currently, there are more than > 850 > subscribers from all over the world. Subscribers include hospital > employees > from major urban centers and obscure remote locales, university > researchers, > botanists and the employees of commercial laboratories, government > agencies, > veterinary facilities and a wide variety of commercial industrial > ventures. > > WHO RUNS HISTONET? > The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using > hardware and software owned by the University of Texas Southwestern > Medical > School, Department of Pathology in Dallas, Texas. If you have any > questions or > problems with Histonet please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us. > > HOW DOES THE LIST WORK? > This server, unlike many systems, uses ONLY ONE ADDRESS to send commands > to > the computer and to post messages. The server will recognize commands sent > in > the SUBJECT line of the message and only when they are spelled exactly as > listed below. Anything not identified as a command will be circulated to > EVERYONE on the list. > > The following is a list of commands the server recognizes: > > subscribe > Your address will be added to the list of subscribers. You will then be > able > to send messages to this list that will be forwarded to all other list > subscribers. You will begin to receive all messages sent to the list by > other > subscribers. > > subscribe digest > Your address will be added to the list of subscribers who receive a > digest > instead of each forwarded message. A digest is a compilation of all the > messages received in a 24 hour period. It is sent to the digest > subscribers > every night after midnight. Digest subscribers can post and respond to > messages the same as "real-time" subscribers. > > digests > A list of available digests will be returned to you. Histonet stores old > messages as daily digests for approximately three months. To read > previous > messages, copy the list of available digests, mark the dates of interest > and > return it to the server. > > unsubscribe > Your address will be removed from the list of subscribers. > You will no longer be able to send messages to the members > of the list. > > help > A list of the commands recognized by the server will be returned to > you. > > WHAT ARE THE RULES? > You may post any questions you wish pertaining to histology, pathology, > in-situ hybridization, immunohistochemistry etc. Equipment and reagent > evaluations, laboratory management issues, government regulations, and job > opportunities are all appropriate topics. The University asks that we > restrict > the use of its hardware and software to business purposes only (occasional > jokes do slip through but PLEASE use restraint). Vendors and those with > commercial interests in histology products are welcome contributors > however, > we ask that blatant advertisements be avoided at all times. It is fine to > refer to product that your company produces if it is pertinent to a topic > being discussed on the list. Unsolicited advertisements are poorly > tolerated > by the members and you will likely receive a number of negative comments > if > you overstep the boundaries. Please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us if you are not sure about the > appropriateness > if a message you wish to post. > > > BASIC HISTONET "NETIQUETTE" > It is most helpful to the list members if you post your responses to > queries > to everyone on the list and not just as a personal reply to the person > asking > the question. That way duplicate messages are minimized and we all learn > from > each other's comments. > > Likewise, if you post a question and get a number of responses back > directly > to you, it is helpful to everyone if you could send out a summary of the > replies you got to Histonet. > > Please avoid abbreviations unless they are explained in your message. For > example: immunohistochemistry (IHC). This list circulates to a wide > variety of > individuals and what seems obvious to you may have no meaning on the other > side of the world. > > Please sign your letter and include your institution or affiliation and > location. Not all email systems have headers which identify the sender. > > Do use the subject line to indicate the topic of your message. > > DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. > > Please send questions and problems about the list directly to Linda > Margraf at > LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 > subscribers on the list. Be careful when sending commands to the server to > put > the command in the SUBJECT LINE and spell it correctly. > > Please do not send images as attachments with your message. We can now > post > images at our web site (http://pathcuri1.swmed.edu). To have an image > posted > send it to Herb Hagler at herb.hagler@email.swmed.edu. > > > > > es > > From mcauliff <@t> umdnj.edu Mon Aug 18 09:18:10 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Re: stealth ads on Histonet?? References: Message-ID: <3F40E022.70603@umdnj.edu> Is it just me or does this sound like an advertisement Vision Biosystems? I wonder if Dr. Elliot Grammon (or is his last name Osterhaus?) has any financial interest in this company? If so, a disclaimer to that effect is warrented. Geoff Elliott Grammon wrote: >Dear Histonetters, > >I found out something very interesting while travelling last week. Apparently, NOVOCASTRA LABORATORIES was purchased in June of last year by VISION BIOSYSTEMS. > >This may not be a big surprise to some, but I also found out that Vision BioSystems has a new large facility just South of Boston, MA where they have been sending out Novocastra antibodies since early 2003. They are building their stock, from what I hear, and many of the more common primaries are delivered overnight anywhere in the Country ! > >Wow! no more waiting for 2 weeks for an antibody as has been the case of late with some Distributors! > >I thought this particularly useful for those on the East Coast who use Novocastra products and don't like the wait they may take coming from California. > >Maybe worth looking into ? www.vision-bio.com > >Good Science ! > >Elliott Osterhaus MD PhD > > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From biovetlab <@t> biovet.se Mon Aug 18 09:12:55 2003 From: biovetlab <@t> biovet.se (Lab BioVet AB) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <009c01c36592$d34a5340$0d64a8c0@telia.com> biovetlab@biovet.se From mward <@t> wfubmc.edu Mon Aug 18 09:20:35 2003 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] test Message-ID: <61135F0455D33347B5AAE209B903A3040326226D@EXCHVS2.medctr.ad.wfubmc.edu> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/aa5a180a/attachment.htm From mike.kirby <@t> nhls.ac.za Mon Aug 18 09:23:08 2003 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Test message from Africa Message-ID: <8431849C2766444FB55D5A373CA3CFDE1D46AD@nhlsmail.nhls.ac.za> From Penguindeb <@t> aol.com Mon Aug 18 09:29:24 2003 From: Penguindeb <@t> aol.com (Penguindeb@aol.com) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] subscribe digest Message-ID: <35640428.09F91EF2.0C4CB7B1@aol.com> Deborah McGee HT,HTL (ASCP) Histology Supervisor Harvard Vanguard Medical Associates Boston,MA 617-421-2308 From kosmicdog <@t> hotmail.com Mon Aug 18 09:34:06 2003 From: kosmicdog <@t> hotmail.com (jason madore) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] permeabilization for nuclear staining? Message-ID: I have a question regarding using detergents to permeabilize tissue for nuclear staining. I suppose that fixing 5 minutes in acetone:EtOH (3:1) will allow access to the cytoplasm, but what about if you are also looking for rare nuclear staining (NF-KB)? I am thinking that the acetone will also open the nucleus but not sure if using a detergent may also be prudent? My guess is probably not but if anyone else has thoughts on this I would like to hear them. ciao, J, Jason Madore Institut du cancer de Montréal Centre de recherché du CHUM 1560 Rue Sherbrooke est Room Y-4609 Montréal, QC H2L 4M1 Canada _________________________________________________________________ Add photos to your e-mail with MSN 8. Get 2 months FREE*. http://join.msn.com/?page=features/featuredemail From N.Lieuwes <@t> orthop.umcn.nl Mon Aug 18 09:58:02 2003 From: N.Lieuwes <@t> orthop.umcn.nl (Lieuwes, N.) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] UNSUBSCRIBE Message-ID: <5B31B9F59E138A409E8F7C2183FF36F017B5CB@umcnet13.umcn.nl> -----Oorspronkelijk bericht----- Van: Herbert, Ronald (NIH/NIEHS) [mailto:herbert@niehs.nih.gov] Verzonden: maandag 18 augustus 2003 16:14 Aan: 'HistoNet@Pathology.swmed.edu' Onderwerp: [Histonet] UNSUBSCRIBE > Your address has been added to the addresses that comprise this Digest > List. > Welcome to HISTONET. This is an electronic mailing list for the exchange > of > information pertaining to histotechnology and related fields. > > PLEASE SAVE THIS MESSAGE. > It contains useful information about how to use the list and what to do if > you > experience problems. It also includes some basic rules for email etiquette > (Netiquette) which will be helpful to those who are new to this form of > communication. > > WHAT IS A LISTSERVER? > A list server is a computer that runs software which will receive incoming > electronic mail (email) messages and reroute them automatically to > everyone on > the subscriber list. Email uses the vast expanse of the Internet to allow > almost instantaneous communication between networked computers around the > world. Our system uses the LISTSTAR software from Quarterdeck Corporation > (California) and can currently send about 30 messages a minute. With the > present number of subscribers, we are processing about 10,000 outbound > messages a day. > > WHO SHOULD SUBSCRIBE? > Anyone interested in research or clinical applications of histology, > immunohistochemistry, in-situ hybridization pathology, and electron > microscopy > may find Histonet informative and useful. Currently, there are more than > 850 > subscribers from all over the world. Subscribers include hospital > employees > from major urban centers and obscure remote locales, university > researchers, > botanists and the employees of commercial laboratories, government > agencies, > veterinary facilities and a wide variety of commercial industrial > ventures. > > WHO RUNS HISTONET? > The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using > hardware and software owned by the University of Texas Southwestern > Medical > School, Department of Pathology in Dallas, Texas. If you have any > questions or > problems with Histonet please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us. > > HOW DOES THE LIST WORK? > This server, unlike many systems, uses ONLY ONE ADDRESS to send commands > to > the computer and to post messages. The server will recognize commands sent > in > the SUBJECT line of the message and only when they are spelled exactly as > listed below. Anything not identified as a command will be circulated to > EVERYONE on the list. > > The following is a list of commands the server recognizes: > > subscribe > Your address will be added to the list of subscribers. You will then be > able > to send messages to this list that will be forwarded to all other list > subscribers. You will begin to receive all messages sent to the list by > other > subscribers. > > subscribe digest > Your address will be added to the list of subscribers who receive a > digest > instead of each forwarded message. A digest is a compilation of all the > messages received in a 24 hour period. It is sent to the digest > subscribers > every night after midnight. Digest subscribers can post and respond to > messages the same as "real-time" subscribers. > > digests > A list of available digests will be returned to you. Histonet stores old > messages as daily digests for approximately three months. To read > previous > messages, copy the list of available digests, mark the dates of interest > and > return it to the server. > > unsubscribe > Your address will be removed from the list of subscribers. > You will no longer be able to send messages to the members > of the list. > > help > A list of the commands recognized by the server will be returned to > you. > > WHAT ARE THE RULES? > You may post any questions you wish pertaining to histology, pathology, > in-situ hybridization, immunohistochemistry etc. Equipment and reagent > evaluations, laboratory management issues, government regulations, and job > opportunities are all appropriate topics. The University asks that we > restrict > the use of its hardware and software to business purposes only (occasional > jokes do slip through but PLEASE use restraint). Vendors and those with > commercial interests in histology products are welcome contributors > however, > we ask that blatant advertisements be avoided at all times. It is fine to > refer to product that your company produces if it is pertinent to a topic > being discussed on the list. Unsolicited advertisements are poorly > tolerated > by the members and you will likely receive a number of negative comments > if > you overstep the boundaries. Please contact Linda Margraf at > LMargraf@childmed.dallas.tx.us if you are not sure about the > appropriateness > if a message you wish to post. > > > BASIC HISTONET "NETIQUETTE" > It is most helpful to the list members if you post your responses to > queries > to everyone on the list and not just as a personal reply to the person > asking > the question. That way duplicate messages are minimized and we all learn > from > each other's comments. > > Likewise, if you post a question and get a number of responses back > directly > to you, it is helpful to everyone if you could send out a summary of the > replies you got to Histonet. > > Please avoid abbreviations unless they are explained in your message. For > example: immunohistochemistry (IHC). This list circulates to a wide > variety of > individuals and what seems obvious to you may have no meaning on the other > side of the world. > > Please sign your letter and include your institution or affiliation and > location. Not all email systems have headers which identify the sender. > > Do use the subject line to indicate the topic of your message. > > DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. > > Please send questions and problems about the list directly to Linda > Margraf at > LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 > subscribers on the list. Be careful when sending commands to the server to > put > the command in the SUBJECT LINE and spell it correctly. > > Please do not send images as attachments with your message. We can now > post > images at our web site (http://pathcuri1.swmed.edu). To have an image > posted > send it to Herb Hagler at herb.hagler@email.swmed.edu. > > > > > es > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LMARGRAF <@t> childmed.dallas.tx.us Mon Aug 18 10:08:43 2003 From: LMARGRAF <@t> childmed.dallas.tx.us (LINDA MARGRAF MD) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: From russther <@t> med.umich.edu Mon Aug 18 10:31:04 2003 From: russther <@t> med.umich.edu (Theresa Russell) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: From Scott.DeVore <@t> dakocytomation.com Mon Aug 18 10:31:14 2003 From: Scott.DeVore <@t> dakocytomation.com (Scott DeVore) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] unsubscribe Message-ID: <349C15307203B445A6B06AA6BA3D425F027BC8E0@EXCHANGE> thanks -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/839c668d/attachment.htm From NoraBRL <@t> aol.com Mon Aug 18 10:40:31 2003 From: NoraBRL <@t> aol.com (NoraBRL@aol.com) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] subscribe digest Message-ID: <137.23cce694.2c724d6f@aol.com> From powell_sa <@t> Mercer.EDU Mon Aug 18 11:21:01 2003 From: powell_sa <@t> Mercer.EDU (Shirley Powell) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] test Message-ID: <001701c365a4$b6e10cf0$a6f2acd1@powellsa1> powell_sa@mercer.edu -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/d6b6d200/attachment.htm From essmancw <@t> BATTELLE.ORG Mon Aug 18 11:31:28 2003 From: essmancw <@t> BATTELLE.ORG (Essman-Wood, Connie) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] SUBSCRIBE DIGEST Message-ID: Connie Essman-Wood Battelle Columbus Operations Pathology Coordinator Phone (614) 424-6522 Fax (614) 424-7441 essmancw@battelle.org From jmaldena <@t> MACNEAL.COM Mon Aug 18 11:33:29 2003 From: jmaldena <@t> MACNEAL.COM (Maldenas, Judy) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Histonet Unsubscribe Message-ID: Judith A. Maldenas, HT (ASCP) Supervisor, Anatomic Pathology Genesis Clinical Laboratory 3231 S Euclid Berwyn, IL 60402 Phone 708-783-0192 Fax 708-783-3794 -------------------------------------------------------------------------------------------------------------------- This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of MacNeal. From algranth <@t> u.arizona.edu Mon Aug 18 11:40:47 2003 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: <4.3.2.7.2.20030818094038.01df6c70@algranth.inbox.email.arizona.edu> ALG ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Robert.Lott <@t> bhsala.com Mon Aug 18 11:47:02 2003 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Deadline for the HT exam Message-ID: <35B6C610DD1DD311B1FA0008C791400407F2FFF2@gobexchm3.bhsala.com> Peggy is exactly correct in describing all the senarios and 5 years cycles applicable to the end of Route #3(high school and two years of experience)in January 2005! You must have applied and sat for the exam at least once. The only thing I will add to her remarks is that the deadline for that application is October 6th, 2004. To qualify you must have your 2 years of full time experience(more than 30 hrs. per week is what the BOR considers "full time"), apply and pay for the HT exam on or prior to the October 6th, 2004 registration deadline. Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388/205-592-5646 (fax) robert.lott@bhsala.com From STapper <@t> slhduluth.com Mon Aug 18 11:48:26 2003 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Ventana Benchmark-HPV users Message-ID: <3BFBBD68413CB443A7125A63EC0ACD1D33A499@slhw2smail01.slhdomain.com> I am wondering if anyone out there could help me with some information my pathologist is looking for. If you are performing HPV testing on the Benchmark, are you putting some sort of disclaimer on your report, since the probe is not IVD? Did you correlate the test to Digene, or did you send the test to a lab that was performing HPV on the Benchmark for your correlations, or both? Thank you! Sheila Tapper HT(ASCP) St. Luke's Hospital -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/975507c3/attachment.htm From STapper <@t> slhduluth.com Mon Aug 18 11:50:18 2003 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Meditech users Message-ID: <3BFBBD68413CB443A7125A63EC0ACD1D33A49A@slhw2smail01.slhdomain.com> Hi - We are currently implementing the Meditech computer system. I am interested in talking with anyone who has experience with building the Histology portion. We are experiencing great difficulty in trying to streamline the entry of specimens with tests attached to the specimens. For example: a liver biopsy with an iron, trichrome and retic stain attached. We would like to have the tests attached to the specimen, and be able to print labels for the slides as well. Can anyone out there help me find the answer? Thanks! Sheila Tapper St. Luke's Hospital Duluth, MN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/a1aab9c8/attachment.htm From silviap <@t> ualberta.ca Mon Aug 18 12:09:35 2003 From: silviap <@t> ualberta.ca (silviap) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] unsuscribe Message-ID: <3F42559F@webmail.ualberta.ca> From ALMINDLS <@t> tuhs.temple.edu Mon Aug 18 12:07:55 2003 From: ALMINDLS <@t> tuhs.temple.edu (Alminde, Lea S) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] test Message-ID: <6A12448FED2ED3119FDB0008C7912CC004CD570E@tuhsms1.tuhis.temple.edu> Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email almindls@tuhs.temple.edu From garygill <@t> dcla.com Mon Aug 18 12:23:17 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Ventana Benchmark-HPV users Message-ID: See No. 4, Bullet 3 in CDRH Guidance for Industry, Analyte Specific Reagents; Small Entity Compliance Guidance. Available at: http://www.fda.gov/cdrh/oivd/guidance/1205.html Gary Gill -----Original Message----- From: Tapper, Sheila [mailto:STapper@slhduluth.com] Sent: Monday, August 18, 2003 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark-HPV users I am wondering if anyone out there could help me with some information my pathologist is looking for. If you are performing HPV testing on the Benchmark, are you putting some sort of disclaimer on your report, since the probe is not IVD? Did you correlate the test to Digene, or did you send the test to a lab that was performing HPV on the Benchmark for your correlations, or both? Thank you! Sheila Tapper HT(ASCP) St. Luke's Hospital -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/08d6bd89/attachment.htm From lesley <@t> vancouverbc.net Mon Aug 18 12:31:47 2003 From: lesley <@t> vancouverbc.net (Lesley Weston) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Re: stealth ads on Histonet?? In-Reply-To: <3F40E022.70603@umdnj.edu> Message-ID: It looked like that to me, certainly, but it's only once (so far) so I didn't worry about it. Lesley Weston. on 18/08/2003 7:18 AM, Geoff McAuliffe at mcauliff@umdnj.edu wrote: > Is it just me or does this sound like an advertisement Vision > Biosystems? I wonder if Dr. Elliot Grammon (or is his last name > Osterhaus?) has any financial interest in this company? If so, a > disclaimer to that effect is warrented. > > Geoff > > Elliott Grammon wrote: > >> Dear Histonetters, >> >> I found out something very interesting while travelling last week. >> Apparently, NOVOCASTRA LABORATORIES was purchased in June of last year by >> VISION BIOSYSTEMS. >> >> This may not be a big surprise to some, but I also found out that Vision >> BioSystems has a new large facility just South of Boston, MA where they have >> been sending out Novocastra antibodies since early 2003. They are building >> their stock, from what I hear, and many of the more common primaries are >> delivered overnight anywhere in the Country ! >> >> Wow! no more waiting for 2 weeks for an antibody as has been the case of late >> with some Distributors! >> >> I thought this particularly useful for those on the East Coast who use >> Novocastra products and don't like the wait they may take coming from >> California. >> >> Maybe worth looking into ? www.vision-bio.com >> >> Good Science ! >> >> Elliott Osterhaus MD PhD >> >> >> >> >> From Stephens.Kimberly <@t> mayo.edu Mon Aug 18 12:36:36 2003 From: Stephens.Kimberly <@t> mayo.edu (Stephens, Kimberly A.) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: <6333378CE48ED211AB5600A0C9EBFDA40B968D6D@excsrv23.mayo.edu> stephens.kimberly@mayo.edu From jluis.palazon <@t> icman.csic.es Mon Aug 18 12:41:37 2003 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] sudan black B Message-ID: <20030818174137.F38DC1EFC0@perceval.uca.es> Dear List members. I need your advice I stained some slides with sudan black B. I used 4 slides per specimen and I did (delipidized, sudan black B, Bromide-sudan black, and bromide-acetone-sudan black). I have noticed that the slides were clearly black after staining, the bromide treated the most black, but after 1-2 weeks, they are turning green in color. Is the color of sudan black B stained slides not permanent? must the slides be read inmediately? or do this change in color alter the results if I read some recently stained slides and other stained some weeks ago?. Is it possible (adequate) to put the coverlips out and re-stain the slides? I would appreciate some advice from you. Thanks in advance José Luis From TMcNemar <@t> lmhealth.org Mon Aug 18 12:44:06 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Meditech users Message-ID: <90092A4ED388D7119575006008F7112049CB27@NT_EXCHANGE> Sheila, I don't know what version you're working with but we have 4.9 and I may be wrong but I don't think you can link procedures to a specific tissue mnemonic. The only way that I know of is to create a new Medical Term for those specimens. At the Term Type prompt, enter TIS (tissue) then on the second screen, you can enter default procedures. You would then enter this as a procedure and it will explode the attached procedures. I have used it for bone marrows. I know it's not quite what you're looking for but entering one procedure is a little better than entering three. As for labels, we don't use them so I can't help you there. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: Tapper, Sheila [mailto:STapper@slhduluth.com] Sent: Monday, August 18, 2003 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech users Hi - We are currently implementing the Meditech computer system. I am interested in talking with anyone who has experience with building the Histology portion. We are experiencing great difficulty in trying to streamline the entry of specimens with tests attached to the specimens. For example: a liver biopsy with an iron, trichrome and retic stain attached. We would like to have the tests attached to the specimen, and be able to print labels for the slides as well. Can anyone out there help me find the answer? Thanks! Sheila Tapper St. Luke's Hospital Duluth, MN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/8f50137f/attachment.htm From turnerd <@t> musc.edu Mon Aug 18 12:52:27 2003 From: turnerd <@t> musc.edu (Debi Turner) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: <1061229147.3f41125b3240f@webmail.musc.edu> turnerd@musc.edu From SJones <@t> cvm.tamu.edu Mon Aug 18 13:00:17 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] cutting MMA at 0.5 microns Message-ID: I'm attempting to cut horse testis embedded in MMA at 0.5 microns. The problem I'm running into is terrible folds in the section. I've tried a dry knife and a wet knife without any luck. I'm using MMA kit 14520 from EMS. Any help would be appreciated. Thanks, Sarah Sarah Jones HT(ASCP) Dept. of Vet. Anatomy & Public Health Histology Lab Texas A&M University College Station, TX 77843-4458 phone: 979-845-3177 fax: 979-458-3499 From SHAKUN.ASWANI <@t> ROCHE.COM Mon Aug 18 13:01:18 2003 From: SHAKUN.ASWANI <@t> ROCHE.COM (Aswani, Shakun {In-V~Palo Alto}) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: <153B66DC1BD39449B2A3A0381FE14DB807CF90@rplmsem1.nala.roche.com> Unsubscribe please Thank you -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/8958c2ea/attachment.htm From huffpw <@t> uleth.ca Mon Aug 18 13:40:44 2003 From: huffpw <@t> uleth.ca (Phillip Huff) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] cutting MMA at 0.5 microns In-Reply-To: References: Message-ID: <2093.142.66.42.91.1061232044.squirrel@webmail.uleth.ca> Just out of curiosity, why do you need your sections so thin? Perhaps you could cut the sections a bit thicker, and use permeabolization methods or other methodologies for your staining. BTW, what methodologies will you be employing on your sections? Phil I'm attempting to cut horse testis embedded in MMA at 0.5 microns. The > problem I'm running into is terrible folds in the section. I've tried a > dry knife and a wet knife without any luck. I'm using MMA kit 14520 > from EMS. Any help would be appreciated. Thanks, Sarah > > Sarah Jones HT(ASCP) > Dept. of Vet. Anatomy & Public Health > Histology Lab > Texas A&M University > College Station, TX 77843-4458 > phone: 979-845-3177 > fax: 979-458-3499 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Mon Aug 18 13:41:57 2003 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Test Message-ID: Richard W. Cartun, Ph.D. Director, Immunopathology Co-Director, Histology Hartford Hospital 80 Seymour Street Hartford, CT 06102 From jtsonger <@t> vt.edu Mon Aug 18 13:50:58 2003 From: jtsonger <@t> vt.edu (Jill Songer) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Chronic wasting disease Message-ID: <5.0.0.25.2.20030818144920.01ecd0a0@pop.vt.edu> Received a suspected case of chronic wasting disease. First one I've had to deal with. Could some kind soul help me out with the regulations/precautions of processing this tissue? Thanks for your help. Jill Songer HT (ASCP) Histopathology Lab Veterinary Medical Teaching Hospital Virginia Tech From RBARNHART <@t> summithealth.org Mon Aug 18 14:13:50 2003 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Meditech users Message-ID: I built the pathology portion of our Meditech Client Server system. There are two ways I have handled this: 1. Have specific specimen types. For example have a liver specimen type that will order the gross level charge and any special stains. The real benifit to this is that you can also attach lab results to the specimen type, as long as the lab is linked to the pathology module. I have all hepatitis and liver function lab results attached to the specimen. This way the pathologist can review the lab results as he is signing out the case. 2. Have a procedure that includes any special stain that is for a liver biopsy. Our sister hospital does a panel of stains for all thier liver biopsies. So I built a profile that includes all the stains and tied the profile to the tissue. This way when they ordered the tissue all the stains were ordered at the same time. With the labels. I was not involved with this part as much. My understanding on our system at least, we can control how many rows of labels we get (each of our rows contain six labels). It is easier for us to reprint labels as needed. I hope this helps. Let me know how else I can help, you can contact me at work or my home email. Becky Barnhart rbarnhart@summithealth.org (work) tbarnhart@innernet.net (home) >>> "Tapper, Sheila" 08/18/03 12:50PM >>> Hi - We are currently implementing the Meditech computer system. I am interested in talking with anyone who has experience with building the Histology portion. We are experiencing great difficulty in trying to streamline the entry of specimens with tests attached to the specimens. For example: a liver biopsy with an iron, trichrome and retic stain attached. We would like to have the tests attached to the specimen, and be able to print labels for the slides as well. Can anyone out there help me find the answer? Thanks! Sheila Tapper St. Luke's Hospital Duluth, MN From hymclab <@t> hyhc.com Mon Aug 18 14:02:49 2003 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Chronic wasting disease Message-ID: <529F3A73499ED611AA9D00A0C9558E4E43EEE8@hyhcexchange.hyhc.local> Jill, You might want to contact Sue Ubl. She set up the Chronic Wasting Disease lab at the Vet division of U of WI. Here is her email: Sue.ubl@wvdl.wisc.edu. Hope this helps, Dawn Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI -----Original Message----- From: Jill Songer [mailto:jtsonger@vt.edu] Sent: Monday, August 18, 2003 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chronic wasting disease Received a suspected case of chronic wasting disease. First one I've had to deal with. Could some kind soul help me out with the regulations/precautions of processing this tissue? Thanks for your help. Jill Songer HT (ASCP) Histopathology Lab Veterinary Medical Teaching Hospital Virginia Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaburns <@t> med.unc.edu Mon Aug 18 14:26:59 2003 From: kaburns <@t> med.unc.edu (Kim Burns) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Subscribe digest Message-ID: <000001c365be$b1dd2e40$0b2e1398@peds.med.unc.edu> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/e9dda0e0/attachment.htm From jeannie_heck <@t> yahoo.com Mon Aug 18 14:27:06 2003 From: jeannie_heck <@t> yahoo.com (Jeannie Heck) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Iron leaching out during decal Message-ID: <20030818192706.31478.qmail@web41605.mail.yahoo.com> Please help! I am working on my HTL Practical test and I am required to do a Prussian Blue iron stain on a 1.0 cm piece of bone. My problem is that everything that I use to decal my bone (so that it cuts nicely) leaches out all of the iron. HCL decal solutions were obviously too powerful (tried them anyways) so I used a milder selection that included acetic acid (after fixing w/ formaldehyde), and a combination of formic acid and formaldehyde (to fix and decal in the same step). I am leary to get Zenker's solution (which was recommended by Frieda Carson's Histology: A Self Instructional Text) because if it doesn't work, then I am left with the expensive and arduous task of disposing of the mercury. Does Zenker's really fix and decal effectively to preserve the iron AND allow me to cut a nice section? What about Zinc Chloride to replace the mercury in Zenker's? Any suggestions would be greatly appreciated, I really need some help. Thank you! Bethany J. Krafels --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/450c6628/attachment.htm From LBlack <@t> carilion.com Mon Aug 18 14:28:33 2003 From: LBlack <@t> carilion.com (Lisa Black) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Test( Message-ID: Lisa Black, B.S., HT(ASCP) Histology Supervisor Carilion Consolidated Laboratory 933 S. Jefferson Street Roanoke, VA 24016 LBLACK@CARILION.COM From SJones <@t> cvm.tamu.edu Mon Aug 18 14:31:35 2003 From: SJones <@t> cvm.tamu.edu (Sarah Jones) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] cutting MMA at 0.5 microns Message-ID: This is for a researcher who is doing apoptag staining. (PMID: 9922213) The reference was for staining in GMA and I thought he might get better results using MMA since the methacrylate can be dissolved away. I think the reason he needs them so thin is to do the cell identification. Sarah >>> Phillip Huff 08/18/03 01:40PM >>> Just out of curiosity, why do you need your sections so thin? Perhaps you could cut the sections a bit thicker, and use permeabolization methods or other methodologies for your staining. BTW, what methodologies will you be employing on your sections? Phil I'm attempting to cut horse testis embedded in MMA at 0.5 microns. The > problem I'm running into is terrible folds in the section. I've tried a > dry knife and a wet knife without any luck. I'm using MMA kit 14520 > from EMS. Any help would be appreciated. Thanks, Sarah > > Sarah Jones HT(ASCP) > Dept. of Vet. Anatomy & Public Health > Histology Lab > Texas A&M University > College Station, TX 77843-4458 > phone: 979-845-3177 > fax: 979-458-3499 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DELONG_CYNTHIA_A <@t> LILLY.COM Mon Aug 18 14:34:04 2003 From: DELONG_CYNTHIA_A <@t> LILLY.COM (DELONG_CYNTHIA_A@LILLY.COM) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Test Message-ID: Cynthia A DeLong LRL - Corporate Center DC0533 - 48B/3042 3055 E Merrill St Indianapolis, IN 46225 phone: 317-276-7635 fax: 317-277-6146 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/a9b92f92/attachment.htm From e.golder-novoselsky <@t> biogenex.com Mon Aug 18 14:41:08 2003 From: e.golder-novoselsky <@t> biogenex.com (Elina Golder-Novoselsky) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] unsubscribe Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/eba29ccd/attachment.htm From jeannie_heck <@t> yahoo.com Mon Aug 18 14:47:38 2003 From: jeannie_heck <@t> yahoo.com (Jeannie Heck) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] GMS Precipitant Message-ID: <20030818194738.11702.qmail@web41608.mail.yahoo.com> The procedure that we use for our Gomori Methenamine Silver (GMS) stain comes directly from Frieda Carson's Histotechnology: A Self Instructional Text. The microwave procedure has worked well for us for many years but about 6 months ago we started getting a dark black precipitant on our slides and the inside of our Coplin jars. We have tried numerous different potential solutions, such as chemically cleaning our Coplin jars, using various types of deionized water (all Type 1), preparing fresh solutions and adjusting the temperature by decreasing and increasing the heating time. When we first encountered this problem it did not occur with the conventional method, but lately it has been occuring with both methods. Any help will be appreciated. Thank you. Jeannie Heck HT (ASCP) --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/06cafd9f/attachment.htm From gcallis <@t> montana.edu Mon Aug 18 14:57:23 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] 0.5 um MMA sections Message-ID: <3.0.6.32.20030818135723.00b611d0@gemini.msu.montana.edu> If you are cutting this thin, the knife must be extremely sharp. Neil Hand cut 1 um PMMA aka MMA with a triangular glass knife and probably NOT a large faced block. It was more like sectioning for EM. You might get by with a very sharp TC knife, otherwise a better knife edge may be necessary. If you are trying to do immunostaining, the MMA would have to be removed as it is so hydrophobic, permeabilization methods will not work. Removal of MMA is easy compared to other plastics. What is the purpose of having sections this thin in the first place? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu From alportbury <@t> yahoo.com Mon Aug 18 15:01:07 2003 From: alportbury <@t> yahoo.com (andrea portbury) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] subscribe digest Message-ID: <20030818200107.6444.qmail@web14806.mail.yahoo.com> --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/e144e886/attachment.htm From gcallis <@t> montana.edu Mon Aug 18 15:04:43 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Iron leaching out during decal In-Reply-To: <20030818192706.31478.qmail@web41605.mail.yahoo.com> Message-ID: <3.0.6.32.20030818140443.00b611d0@gemini.msu.montana.edu> Jeannie, This has been discussed on Histonet in the past, so do search also. If you are using a heavy duty iron positive control, i.e. liver with lots of iron, you may confuse the issue. We used to decalcify with formic acid or HCL and were very careful not to overexpose the bone marrow to acid. We retained iron but not at the high level you see with a positive tissue control, bone marrow cells containing iron tend to be subtle and fewer in number, but iron is still there - you have to look carefully to find it unless you have a case that shows high iron stores in cells. Do't use EDTA, it chelates iron! At 12:27 PM 8/18/2003 -0700, you wrote: >Please help! I am working on my HTL Practical test and I am required to do >a Prussian Blue iron stain on a 1.0 cm piece of bone. My problem is that >everything that I use to decal my bone (so that it cuts nicely) leaches out >all of the iron. HCL decal solutions were obviously too powerful (tried >them anyways) so I used a milder selection that included acetic acid (after >fixing w/ formaldehyde), and a combination of formic acid and formaldehyde >(to fix and decal in the same step). I am leary to get Zenker's solution >(which was recommended by Frieda Carson's Histology: A Self Instructional >Text Chloride to replace the mercury in Zenker's? Any suggestions would be >greatly appreciated, I really need some help. Thank you! Bethany J. >Krafels Do you Yahoo!? > Yahoo! SiteBuilder - Free, easy-to-use web site design software Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu From gcallis <@t> montana.edu Mon Aug 18 15:11:51 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] GMS Precipitant In-Reply-To: <20030818194738.11702.qmail@web41608.mail.yahoo.com> Message-ID: <3.0.6.32.20030818141151.00b611d0@gemini.msu.montana.edu> We always made up fresh STOCK solution of Methenamine silver when this occured - be sure to check you stock solution even if it is a long way from expiration day. If it has any cloudiness or you see deposition on side of stock bottle, make up new. If the stock solution is bad, your newly made up working solution will be bad too. We also store our silver nitrate in the refrigerator. At 12:47 PM 8/18/2003 -0700, you wrote: > Gomori Methenamine Silver (GMS) stain comes directly from Frieda Carson's >Histotechnology: A Self Instructional Text our Coplin jars. We have tried >numerous different potential solutions, such as chemically cleaning our >Coplin jars, using various types of deionized water (all Type 1), preparing >fresh solutions and adjusting the temperature by decreasing and increasing >the heating time. When we first encountered this problem it did not occur >with the conventional method, but lately it has been occuring with both >methods. Any help will be appreciated. Thank you. Jeannie Heck HT (ASCP) >Do you Yahoo!? > Yahoo! SiteBuilder - Free, easy-to-use web site design software Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu From sschu <@t> Arctur.com Mon Aug 18 15:17:01 2003 From: sschu <@t> Arctur.com (Shirley S. Chu) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Subscribe digest Message-ID: From vbaker60 <@t> yahoo.com Mon Aug 18 15:19:47 2003 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] B+ fixative Message-ID: <20030818201947.37940.qmail@web12106.mail.yahoo.com> Hi Everyone, Does anyone out there use a B-5 fixative subsitute called B+?? I need to know what they are using in place of the mercuric chloride if possible. I'm not getting much feedback from the company. Thanks, Vikki Baker Institute for Cancer Prevention Valhalla, NY 10595 __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software http://sitebuilder.yahoo.com From Wanda.Smith <@t> HCAhealthcare.com Mon Aug 18 15:23:32 2003 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Test Message-ID: <8784A3EE34199644AF60DBE517F5B0A6025DBBE7@louex04.lou.medcity.net> > Wanda G. Smith, HTL/HT(ASCP) > Pathology Supervisor > Trident Medical Center Laboratory Services > *9330 Medical Plaza Drive, Charleston, SC 29406 > *843-797-4586 *fax 843-797-4296 > *wanda.smith@hcahealthcare.com > > > From Jenny-Oblander <@t> omrf.ouhsc.edu Mon Aug 18 15:41:06 2003 From: Jenny-Oblander <@t> omrf.ouhsc.edu (Jenny Oblander) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Test Message-ID: J.Oblander, HT (A.S.C.P.) Comparative Medicine Oklahoma Medical Research Foundation MS#32 825 NE 13th St. Okc,Ok 73104 jenny-oblander@omrf.ouhsc.edu 405-271-7083 From David.Muskett <@t> btopenworld.com Mon Aug 18 15:48:26 2003 From: David.Muskett <@t> btopenworld.com (David Muskett) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Training for Staff Message-ID: Dear Histonetters I am currently looking to revamp the training programme in my department. Does anyone have any material or old exam questions they are willing to share with me. David Muskett Liverpool, UK From Cathy.Crumpton <@t> tuality.org Mon Aug 18 16:03:11 2003 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Re: Decal Leaching Iron Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/7b89aa3f/attachment.htm From LMARGRAF <@t> childmed.dallas.tx.us Mon Aug 18 16:06:15 2003 From: LMARGRAF <@t> childmed.dallas.tx.us (LINDA MARGRAF MD) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] New Histonet Listserver IMPORTANT INFORMATION Message-ID: Dear Histonetters: As you have all likely noticed we have changed the Histonet server to a new and actually better program (our University made changes to the computers which made this change manditory). Herb Hagler diligently transfered everyone from the old list to the new one. PLEASE NOTE THE IMPORTANT CHANGE: In the new system, to make changes to your subscription you now need to go to the website address that was in the intro message that was sent to you this morning. YOU CAN NO LONGER PUT A COMMAND IN THE SUBJECT OF A MESSAGE TO MAKE A CHANGE TO YOUR SUBSCRIPTION. At the website you can switch to the digest mode. There are other useful features including an archive, ways to stop mail for vacations etc. The digest mode will be more user friendly and readable. Unfortunately, the web site was down this am but it is back up now so for those of you trying to change to digest, please try now or later today. Give it a few days or so for everything to get settled then let me know if you are having problems. Sorry about the inconvenience. Linda M Histonet administrator -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/a0748a71/attachment.htm From nick.kirk3 <@t> btopenworld.com Mon Aug 18 16:09:57 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Training for Staff In-Reply-To: Message-ID: David Is this a training programme for HPC State Registration? If so, I have produced a 100+ page training manual to be used in conjunction with the Logbook. It's broken down into sections (microtomy, stains, processing etc) and is basically a question and answer book where the trainee can write down answers or draw diagrams to help with their technical training. It does need a bit of updating in places, but your welcome to a copy if you would like one and you can plagiarise it to your hearts content (or throw it in the bin). Let me know and I'll email you a copy. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of David Muskett Sent: 18 August 2003 21:48 To: Histonet Subject: [Histonet] Training for Staff Dear Histonetters I am currently looking to revamp the training programme in my department. Does anyone have any material or old exam questions they are willing to share with me. David Muskett Liverpool, UK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From e.golder-novoselsky <@t> biogenex.com Mon Aug 18 16:27:35 2003 From: e.golder-novoselsky <@t> biogenex.com (Elina Golder-Novoselsky) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] unsubscribe Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/25347960/attachment.htm From lpstirpe <@t> yahoo.com Mon Aug 18 18:51:41 2003 From: lpstirpe <@t> yahoo.com (Linda Stirpe) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Test Message-ID: <20030818235141.37736.qmail@web40310.mail.yahoo.com> __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software http://sitebuilder.yahoo.com From ctanck <@t> juno.com Mon Aug 18 20:01:57 2003 From: ctanck <@t> juno.com (Carol J Tanck) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] test Message-ID: <20030818.210158.-665573.0.ctanck@juno.com> ________________________________________________________________ The best thing to hit the internet in years - Juno SpeedBand! Surf the web up to FIVE TIMES FASTER! Only $14.95/ month - visit www.juno.com to sign up today! From WWmn916 <@t> aol.com Mon Aug 18 20:25:57 2003 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Fwd: Hale's Colloidal Iron Message-ID: Skipped content of type multipart/alternative-------------- next part -------------- An embedded message was scrubbed... From: WWmn916@aol.com Subject: Hale's Colloidal Iron Date: Sat, 16 Aug 2003 11:50:36 EDT Size: 2682 Url: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/753dd903/attachment.eml From portera203 <@t> yahoo.com Mon Aug 18 20:27:22 2003 From: portera203 <@t> yahoo.com (Amy Porter) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Just another Test Message-ID: <20030819012722.6459.qmail@web40911.mail.yahoo.com> Just getting used to everything new .... Amy S.Porter, HT(ASCP) Michigan State University Department of Physiology Division of Human Pathology College of Human Medicine portera203@yahoo.com --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030818/90aaba49/attachment.htm From RSRICHMOND <@t> aol.com Mon Aug 18 22:16:51 2003 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Re: Iron leaching out during decal Message-ID: <68.33b23d98.2c72f0a3@aol.com> Bethany J. Krafels describes difficulty in >>HTL Practical test and I am required to do a Prussian Blue iron stain on a 1.0 cm piece of bone.<< I presume that you're allowed to use cancellous bone (the relatively soft bone of the interior of the vertebral bones, with red marrow) since that's what's normally stained for iron. This specimen needs to be obtained at autopsy, from a patient with presumably normal iron stores (the pathologist doing the autopsy should know this), slabbed out of a lumbar vertebral body with a hand saw, about 2 mm thick (this would take me a couple of minutes to do, and is something I'd probably do anyway). The slab should be fixed overnight (or a little longer) in neutral buffered formalin. Most of the standard decalcifying agents should decalcify it in the course of a working day, or overnight; check it, and don't leave it in the decalcifier any longer than needed. Take it from there like any other piece of tissue. If it doesn't work the first time, try it again with another case. Zenker's fixative is the queen of battle for marrow morphology, but is not an ideal fixative for hemosiderin iron, because it's acid enough to leach some of it out. Neutral buffered formalin is exactly the fixative you need. Bob Richmond Samurai Pathologist Knoxville TN From bruyntjes <@t> voeding.tno.nl Tue Aug 19 01:59:17 2003 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] subscribe digest Message-ID: <3B070848E7C2204F9DEB8BCFD76772800210D40F@ntexch1.voeding.tno.nl> Joost Bruijntjes TNO Nutrition and Food Research PO Box 360 3700 AJ Zeist The Netherlands From Hedley.Glencross <@t> CMMC.nhs.uk Tue Aug 19 02:13:56 2003 From: Hedley.Glencross <@t> CMMC.nhs.uk (Glencross Hedley (RW3) CM&MC Manchester) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] histonet Message-ID: Hi Linda/Herb After some unsuccesful attempts to change my subscriptions via the website (I see it was down for sometime), I'm now running digest mode. As you indicate its much more user friendly, and I do not have to trawl through all the messages anymore. Thanks Hedley Glencross From elizabeta.mukaetova-ladinska <@t> ncl.ac.uk Tue Aug 19 10:38:40 2003 From: elizabeta.mukaetova-ladinska <@t> ncl.ac.uk (Elizabeta Mukaetova-Ladinska) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] unsubscribe Message-ID: <352F86C0-D25B-11D7-8C65-000A959F0044@ncl.ac.uk> From lpwenk <@t> mail.netquest.com Tue Aug 19 03:50:23 2003 From: lpwenk <@t> mail.netquest.com (Lee & Peggy Wenk) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Iron leaching out during decal References: <20030818192706.31478.qmail@web41605.mail.yahoo.com> Message-ID: <00c201c3662e$ede5e060$8732fea9@hppav> 1. Make certain that it is hematopoetic bone. That is, bone with marrow (RBC, WBC, etc.). Vertebrae, rib, or sternum are fine. But NOT adult femur heads, or bones from arms or legs from amputations from adults. These are all fat and connective tissue. No iron stores in these bones in adults. 2. Use a mild decal for minimal time. We like to use FASC (formic acid, sodium citrate) for 2-3 days. Stronger acids tend to leach out the iron stores. If using hydrochloric or nitric acids, use 5% for a few hours to overnight, depending upon the size (see #3). 3. Use minimum size. The larger the size you gross, the longer it has to be in decal, and the more iron that gets leached out. The HTL exam asks for bone 1 cm long. There is no requirement for width. So at needle core biopsy would be acceptable, as long as there is cortex and hematopoetic marrow. 4. Realize that not all adults have large amounts of iron stores. If you are getting it from an autopsy from someone who has been sick a long time, or who has been undergoing treatment for cancer, they may have very little to no iron stores. Try several different sources. 5. Make certain the formalin is OK. Acidic formalin will also leech out the iron, so use newly made, buffered formalin. And fix for minimal time. If the bone is being stored in formalin for a length of time (weeks to months), the formalin can go acidic also. Just some hints we've learned along the way. Peggy A. Wenk, HTL(ASCP)SLS School of Histologic Technicians and Histotechnologists William Beaumont Hospital Royal Oak, MI ----- Original Message ----- From: Jeannie Heck To: Histonet Sent: Monday, August 18, 2003 3:27 PM Subject: [Histonet] Iron leaching out during decal Please help! I am working on my HTL Practical test and I am required to do a Prussian Blue iron stain on a 1.0 cm piece of bone. My problem is that everything that I use to decal my bone (so that it cuts nicely) leaches out all of the iron. HCL decal solutions were obviously too powerful (tried them anyways) so I used a milder selection that included acetic acid (after fixing w/ formaldehyde), and a combination of formic acid and formaldehyde (to fix and decal in the same step). I am leary to get Zenker's solution (which was recommended by Frieda Carson's Histology: A Self Instructional Text) because if it doesn't work, then I am left with the expensive and arduous task of disposing of the mercury. Does Zenker's really fix and decal effectively to preserve the iron AND allow me to cut a nice section? What about Zinc Chloride to replace the mercury in Zenker's? Any suggestions would be greatly appreciated, I really need some help. Thank you! Bethany J. Krafels ------------------------------------------------------------------------------ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/59d69940/attachment.htm From lpwenk <@t> mail.netquest.com Tue Aug 19 03:59:52 2003 From: lpwenk <@t> mail.netquest.com (Lee & Peggy Wenk) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] GMS Precipitant References: <20030818194738.11702.qmail@web41608.mail.yahoo.com> Message-ID: <00cd01c36630$40cdd340$8732fea9@hppav> Any chance you got in a new batch of silver nitrate about 6 months ago? This has happened to us before. In both instances, it ended up being a poor grade of silver nitrate. In one case, the was the fault of the company (poor QC). We returned it to the company and they gave us another batch/lot number, which worked fine. Another time, our hospital purchasing department tried to save money by switching to a cheaper grade of silver nitrate, which also precipitated all the time, too. In the long run, it didn't save money and in fact cost us more (see next paragraph). In the meantime, make up a double batch (fresh). Place in two different coplin jars. Heat the slide in one coplin jar with silver. When that silver solutions starts turning blackish, pull the slide out and place it in the other coplin jar of silver solution that has been sitting on the counter. Now heat the second container of silver solution with the slide in it. By the time that silver starts precipitating, your slide/fungus should be about the right shade of gray/black. (Now you see why the poor grade of silver ended up costing us more money in the long run. We had to make two coplin jars of silver solution, instead of one.) Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: Jeannie Heck To: Histonet Sent: Monday, August 18, 2003 3:47 PM Subject: [Histonet] GMS Precipitant The procedure that we use for our Gomori Methenamine Silver (GMS) stain comes directly from Frieda Carson's Histotechnology: A Self Instructional Text. The microwave procedure has worked well for us for many years but about 6 months ago we started getting a dark black precipitant on our slides and the inside of our Coplin jars. We have tried numerous different potential solutions, such as chemically cleaning our Coplin jars, using various types of deionized water (all Type 1), preparing fresh solutions and adjusting the temperature by decreasing and increasing the heating time. When we first encountered this problem it did not occur with the conventional method, but lately it has been occuring with both methods. Any help will be appreciated. Thank you. Jeannie Heck HT (ASCP) ------------------------------------------------------------------------------ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/1eb3bb50/attachment.htm From Diane.Gladney <@t> se.amedd.army.mil Tue Aug 19 04:51:03 2003 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] B+ fixative Message-ID: <9D41AB7C56F8304F98537ABD87B249F6626112@dasmthgbz001.amedd.army.mil> Vikki, We use B+ fixative for our lymph nodes. It does a wonderful job and our pathologists are very pleased with it. So far, this has been the best B-5 substitute that we have ever used. I highly recommend it. No more having to remove mercury pigment!!! Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: Victoria Baker [mailto:vbaker60@yahoo.com] Sent: Monday, August 18, 2003 4:20 PM To: HistoNet Server Subject: [Histonet] B+ fixative Hi Everyone, Does anyone out there use a B-5 fixative subsitute called B+?? I need to know what they are using in place of the mercuric chloride if possible. I'm not getting much feedback from the company. Thanks, Vikki Baker Institute for Cancer Prevention Valhalla, NY 10595 __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software http://sitebuilder.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Tue Aug 19 05:08:42 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Eosinophils. Message-ID: <5.2.1.1.2.20030819110652.00a02a90@udcf.gla.ac.uk> IHC markers for mouse eosinophils, suggestions, suppliers and advice welcome. Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/0a681922/attachment.htm From Tbarnhart <@t> primecare.org Tue Aug 19 06:39:54 2003 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] A big thank you Message-ID: <1779904B5E82D511914C00D0B79333922053F1@exchangent> Linda, Herb and anyone else who has helped with this list server, I would just like to say thank you for all your hard work with the Histonet. This has become a very valuable tool for the histology community and has helped the field tremendously. We are all forever in your debt. Again, THANK YOU! Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND tbarnhart@primecare.org Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From hmcleod <@t> chempath.uct.ac.za Tue Aug 19 07:01:02 2003 From: hmcleod <@t> chempath.uct.ac.za (Mcleod) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] test Message-ID: <3F42117E.616F5A2D@chempath.uct.ac.za> testing From rstapf <@t> adventisthealthcare.com Tue Aug 19 07:06:56 2003 From: rstapf <@t> adventisthealthcare.com (Ross Stapf) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Histology Supervisor openning Message-ID: Hello everyone: I announced my resignation as supervisor here at Washington Adventist Hospital yesterday. I will be leaving to become the Histology Manager at Baylor University Medical Center. Therefore my current position will be open. The successful candidate will supervise a great group of histotechs. Many exciting plans are in the works to bring in new technologies. We currently do about 15,000 to 16,000 slides a month and about 2500 immuno's. We have 2 Dako stainers (and are in negotiations for a third), automated coverslipper, automated stainer, etc. A person in histology is being trained to be the interim supervisor and will be able to help with the transition for the new supervisor. This is a great group of people to work with, The techs, the Pathologists, and management have all been very supportive to me. I would not be leaving, but I received an offer I could not refuse. Please Send resume's to Dr Nicolas Cacciabeve. His email address is CC'ed to this email. They can also be faxed to the fax number below. If you need more information, please let me know. Ross Stapf Histology Supervisor Washington Adventist Hospital Takoma Park MD 301-891-6102 (office) 301-891-5141 (fax) -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/786c997d/attachment.htm From d.gregg <@t> juno.com Tue Aug 19 07:41:31 2003 From: d.gregg <@t> juno.com (Douglas A Gregg) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] subscribe digest Message-ID: <20030819.084132.1820.1.d.gregg@juno.com> From ctilton <@t> eriesci.com Tue Aug 19 07:43:58 2003 From: ctilton <@t> eriesci.com (Tilton, Cindy - Erie) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] subscribe digest Message-ID: <0377F16E0F96D411A0B000B0D049A97E02633617@usnh003nt02.eriesci.com> ********************************************************************** This email message, and any files transmitted with it, contain confidential and/or legally privileged information and are intended solely for the person(s) to whom this email message is addressed. Any review, transmission, disclosure, copying, or other action taken or not taken with respect to this email message, or the files transmitted with it, by any person other than the person(s) to whom this email message is addressed is prohibited. If you have received this email message in error, please notify the sender immediately by telephone or email and destroy all versions of the original message without making a copy. Please be advised that Erie Scientific Company reserves the right to monitor, access, and review all messages, data, and images transmitted through our email system. By using our email system, you consent to this monitoring. Thank you. From cdemarinis <@t> SARATOGACARE.ORG Tue Aug 19 08:08:05 2003 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis,Carolyn) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] Attach lab results to specimen type Message-ID: <2FCF6059F121BA41A672C5012BB113AA2A5293@mercury.sarahosp.org> In response to Becky Barnhart's email on Meditech Client Server system: how do you attach hepatitis and liver function lab results to the specimen type "liver"? We have Meditech MAGIC and I see no way in the "specimen type" dictionary to do this. Thanks. CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From arme <@t> optonline.net Tue Aug 19 08:18:51 2003 From: arme <@t> optonline.net (Mark Sofferman) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] unsubscribe In-Reply-To: Message-ID: -----Original Message----- From: HistoNet Server [mailto:histonet@pathology.swmed.edu] Sent: Tuesday, August 05, 2003 11:15 AM To: HistoNet Server Subject: Daily Digest ---------------------------------------------------------------------- Date: 4 Aug 2003 03:23:18 -0500 From: Chris van der Loos Subject: RE: more info again, End PO block Dear Becky, There is a new endogenous blocking solution from Dako called "Dual endogenous enzyme block" (S2003). It will kill both endogenous peroxidase and alkaline phosphatase activities in one 10 min incubation step prior to your immunosteps. As this stuff is new my experience is only limited. So far it seems very effective without damaging the tissue epitopes. Furthermore, as Gayle Callis already wrote, methanol + 0.3% peroxide (20 min, RT) is very effective to block endogenous peroxidase activity in FFPE sections. Because those sections have been trough alcohols several times during embedding and dewaxing, the methanol has no additional damaging effect. Be aware that methanol can be very damaging to many tissue epitopes when immunostaining cryostat sections! I have also tried to prolong the incubation time of a lesser effective endogenous peroxidase blocking method as you wrote in your first mail, but it didn't help. Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands At 11:47 AM 8/1/2003 -0400, you wrote: >I'm sorry about not giving enough information. I have had several >responses asking for more details. So, I'll try again! > >I am performing IHC staining. I am using a DAKO autostainer with >the LSAB detection system. I use DAB for my chromagen. My negative >mouse and negative rabbit serum slides have quite specific staining >in the granlocytic series of WBC's(No matter what pretreatment - >I apply no pretreatment, Proteinase K, or use Microwave at pH 6 and >10). Microwave pretreatment is the worst, but I get the staining >most of the time. Polysegmented neutrophils seem to be the main >culprit. Most of the times my Docs can read through this, but they >are requesting my experimentation to find a "cure" for the situation, >so they can honestly say, "no staining was seen in the negative >control". One of the suggestions I have received is to do an Avidin/Biotin >Block. I will give this a try, but if there are any other ideas out >there: DON'T hold back! THANKS AGAIN! ---------------------------------------------------------------------- Date: 4 Aug 2003 05:05:13 -0500 From: "louise renton" Subject: Re: coated slides Elmer's Dear all I have heard a lot about Elmer's Glue, which I gather is some type of household vinyl based adhesive. We don't get this specific brand, but similar ones, used specifically for woodwork or crafts are available. So...my question is this: How is it used? Neat, Diluted, in the waterbath??? PLease advise as I have some deadful whole paw sections that I am having difficuly "sticking" to the slide. BTW, I have tried chrome alum, but get horrible background so my thanks go to Gayle for suggesting placing them in NBF. I will try that too. Best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" - ----At 08:48 a.m. 01/08/2003 -0400, you wrote: >Hi, >I am having problems with tissue staying on my slides after drying >overnight >in 37C oven and then one more night in a 60C oven. The tissue is chicken >leg knee joints. They are formalin fixed, EDTA decaled, and paraffin >embedded samples. I think that coated slides may help. Does anyone have a >simple protocol for making coated slides to help this tissue stick? >Thanks in advance for the help. > >Loralee Gehan >University of Rochester > Loralle: You can use a vinyl glue like Elmer#180#s, it works fine in cases like you are describiyng. The adhesion properties are at least the same that charged slides when you are treating samples like cartilage and bone. Good luck,Carlos. _________________________________________________________________ Unsatisfied with being single? Try MSN Personals http://www.msn.co.za/personals/ ---------------------------------------------------------------------- Date: 4 Aug 2003 10:01:46 -0500 From: Gayle Callis Subject: Caveat about Elmer's glue Elmers glue has been used in the past, but some labs reported contamination (bacterial, whatever) when using it - and if I remember correctly, there have been publications/technical hints about this problem. That was enough for our lab to abandon Elmers glue in lieu of in house prepared chrome subbing solutions or (way back then) advent of poly l lysine or silane coatings. We use Elmers glue for a short time, but disliked its white opacity. I was under the impression Elmers was a by product of the dairy industry, since Bordens (of Elsie the Cow fame) manufactured this glue for school children and household use. Personally, I would rather purchase commercial(Surgipath) or make up chrome gelatin solution or some other slide coating solution for histology purposes and leave Elmers to the school children and households. When poly l lysine coating methods arrived on the scene, we abandoned Elmers glue entirely or did the chrome gelatin subbing for the very difficult, large decalcified sheep tibia, dog tibia and femur section. If you want to reduce background and are using 275 bloom gelatin, go to 100 bloom, larger molecule collagen often gives more H&E background staining than smaller molecule (100 bloom) gelatin. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu ---------------------------------------------------------------------- Date: 4 Aug 2003 10:21:54 -0500 From: "DiCarlo, Margaret" Subject: RE: coated slides Elmer's Louise, Fred told me about Titebond II which is an adhesive found in any local hardware store. Just dilute it to make a 5% solution. I drop a few drops on my slide using a disposable pipet and spread all over using kimwipe and air dry it right before use and I have been having great results with it. There is no background staining from using it. I hope you try this. Good luck. Peggy DiCarlo HT(ASCP) Orthopedics Bone Lab Buffalo General Hospital - -----Original Message----- From: louise renton [mailto:louise_renton@hotmail.com] Sent: Monday, August 04, 2003 06:03 To: histonet@pathology.swmed.edu Subject: Re: coated slides Elmer's Dear all I have heard a lot about Elmer's Glue, which I gather is some type of household vinyl based adhesive. We don't get this specific brand, but similar ones, used specifically for woodwork or crafts are available. So...my question is this: How is it used? Neat, Diluted, in the waterbath??? PLease advise as I have some deadful whole paw sections that I am having difficuly "sticking" to the slide. BTW, I have tried chrome alum, but get horrible background so my thanks go to Gayle for suggesting placing them in NBF. I will try that too. Best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" - ----At 08:48 a.m. 01/08/2003 -0400, you wrote: >Hi, >I am having problems with tissue staying on my slides after drying >overnight >in 37C oven and then one more night in a 60C oven. The tissue is chicken >leg knee joints. They are formalin fixed, EDTA decaled, and paraffin >embedded samples. I think that coated slides may help. Does anyone have a >simple protocol for making coated slides to help this tissue stick? >Thanks in advance for the help. > >Loralee Gehan >University of Rochester > Loralle: You can use a vinyl glue like Elmer#180#s, it works fine in cases like you are describiyng. The adhesion properties are at least the same that charged slides when you are treating samples like cartilage and bone. Good luck,Carlos. _________________________________________________________________ Unsatisfied with being single? Try MSN Personals http://www.msn.co.za/personals/ CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. ---------------------------------------------------------------------- Date: 4 Aug 2003 10:47:21 -0500 From: Nick_Madary@hgsi.com Subject: Antibody for Anthrax Spores? I am sure this has been discussed before but are there any commercially available antibodies for anthrax spores? ******************* NOTE ******************* There may be important message content contained in the following MIME Information. ******************************************** - ------------------ MIME Information follows ------------------ This is a multipart message in MIME format. - --=_alternative 0056194085256D78_= Content-Type: text/plain; charset="us-ascii" <<<<<< See above "Message Body" >>>>>> - --=_alternative 0056194085256D78_= Content-Type: text/html; charset="us-ascii"
I am sure this has been discussed before but are there any commercially available antibodies for anthrax spores? - --=_alternative 0056194085256D78_=-- ---------------------------------------------------------------------- Date: 4 Aug 2003 11:14:19 -0500 From: "Smith, Allen" Subject: RE: coated slides Elmer's Since Elmer's is made by Borden's (a dairy company), it is probably casein. Allen A. Smith, Ph.D. Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161-6695 - -----Original Message----- From: louise renton [mailto:louise_renton@hotmail.com] Sent: Monday, August 04, 2003 6:03 AM To: histonet@pathology.swmed.edu Subject: Re: coated slides Elmer's Dear all I have heard a lot about Elmer's Glue, which I gather is some type of household vinyl based adhesive. We don't get this specific brand, but similar ones, used specifically for woodwork or crafts are available. So...my question is this: How is it used? Neat, Diluted, in the waterbath??? PLease advise as I have some deadful whole paw sections that I am having difficuly "sticking" to the slide. BTW, I have tried chrome alum, but get horrible background so my thanks go to Gayle for suggesting placing them in NBF. I will try that too. Best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" - ----At 08:48 a.m. 01/08/2003 -0400, you wrote: >Hi, >I am having problems with tissue staying on my slides after drying >overnight >in 37C oven and then one more night in a 60C oven. The tissue is chicken >leg knee joints. They are formalin fixed, EDTA decaled, and paraffin >embedded samples. I think that coated slides may help. Does anyone have a >simple protocol for making coated slides to help this tissue stick? >Thanks in advance for the help. > >Loralee Gehan >University of Rochester > Loralle: You can use a vinyl glue like Elmer#180#s, it works fine in cases like you are describiyng. The adhesion properties are at least the same that charged slides when you are treating samples like cartilage and bone. Good luck,Carlos. _________________________________________________________________ Unsatisfied with being single? Try MSN Personals http://www.msn.co.za/personals/ The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ---------------------------------------------------------------------- Date: 4 Aug 2003 11:57:39 -0500 From: "Cathrine Dreanno" Subject: RDO-gold and ISH Hi, I would like to decalcify my samples and do some in situ hybridization. I read that RDO is not suitable for ISH (Alers et al., 1999, 47; 703-709, JHC). They have a new product called "RDO-gold". They say the "nucleic acid are altered". Does anyone have any experience of this product ? Thank you for advices , Catherine ---------------------------------------------------------------------- Date: 4 Aug 2003 12:20:35 -0500 From: Margaret_McKinney@brown.edu Subject: flash freezing, brain, and isopentane I have a quick question regarding flash freezing of rat brain in freezing isopentane. Does anyone have an opinion on flash-freezing by simply dropping the brain into the isopentane versus placing it in a container with OCT or similar media and then placing this into the isopentane? I have been running into problems with both micro and macro cracking of the tissue. Thanks, Margaret McKinney. Margaret McKinney Brown University Medical School 97 Waterman St. Providence, RI 02912 ---------------------------------------------------------------------- Date: 4 Aug 2003 12:48:52 -0500 From: Judi Ford Subject: Gallyas Stain on Brain Hi, I've just finished doing the Gallyas stain for tangles and have found that no matter how gentle I am with the slides tissue falls off the slide during the stain. I thought about using + slides but heard that brain tissue and plus slides don't work due to +/- charges working against each other. Any suggestions? Also, what could I use as a control for tangles? I'm working with mouse tissue. Thanks for your help. Judi Ford Histotechnologist Jackson Laboratory, ME ---------------------------------------------------------------------- Date: 4 Aug 2003 12:49:14 -0500 From: "Dawson, Glen" Subject: MIB-1 (Ki-67) VENDOR All, I am looking for the MIB-1 (Ki-67) antibody that used to be sold by Immunotech (Cat. #IM0505). Unless I'm mistaken, Immunotech is no longer around but this antibody is/was the best. Any leads on who may actually have this particular antibody or who may have bought out immunotech would be much appreciated. Thanx in Advance, Glen A. Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI ---------------------------------------------------------------------- Date: 4 Aug 2003 12:49:41 -0500 From: Nick_Madary@hgsi.com Subject: Re: flash freezing, brain, and isopentane Snap freeze the whole brain as it is almost its own "oct" type matrix. You wouls still want to use the oct as a method for sticking the brain onto the chuck or block holder. Margaret_McKinney@brown.edu 08/04/03 12:16 PM To: histonet@pathology.swmed.edu cc: Subject: flash freezing, brain, and isopentane I have a quick question regarding flash freezing of rat brain in freezing isopentane. Does anyone have an opinion on flash-freezing by simply dropping the brain into the isopentane versus placing it in a container with OCT or similar media and then placing this into the isopentane? I have been running into problems with both micro and macro cracking of the tissue. Thanks, Margaret McKinney. Margaret McKinney Brown University Medical School 97 Waterman St. Providence, RI 02912 ******************* NOTE ******************* There may be important message content contained in the following MIME Information. ******************************************** - ------------------ MIME Information follows ------------------ This is a multipart message in MIME format. - --=_alternative 005E974405256D78_= Content-Type: text/plain; charset="us-ascii" <<<<<< See above "Message Body" >>>>>> - --=_alternative 005E974405256D78_= Content-Type: text/html; charset="us-ascii"
Snap freeze the whole brain as it is almost its own "oct" type matrix. You wouls still want to use the oct as a method for sticking the brain onto the chuck or block holder.


Margaret_McKinney@brown.edu

08/04/03 12:16 PM

       
        To:        histonet@pathology.swmed.edu
        cc:        
        Subject:        flash freezing, brain, and isopentane



I have a quick question regarding flash freezing of rat brain in freezing isopentane. Does anyone have an opinion on flash-freezing by simply dropping the brain into the isopentane versus placing it in a container with OCT or similar media and then placing this into the isopentane? I have been running into problems with both micro and macro cracking of the tissue.

Thanks,
Margaret McKinney.

Margaret McKinney
Brown University Medical School
97 Waterman St.
Providence, RI 02912

- --=_alternative 005E974405256D78_=-- ---------------------------------------------------------------------- Date: 4 Aug 2003 13:58:50 -0500 From: "Jocelyn Torcolini" Subject: RE: unsubscribe ---------------------------------------------------------------------- Date: 4 Aug 2003 14:00:12 -0500 From: "Bryan Hewlett" Subject: Re: MIB-1 (Ki-67) VENDOR Glen, This clone is available from DakoCytomation. Bryan - ----- Original Message ----- From: "Dawson, Glen" To: Sent: Monday, August 04, 2003 1:37 PM Subject: MIB-1 (Ki-67) VENDOR > > All, > > I am looking for the MIB-1 (Ki-67) antibody that used to be sold by > Immunotech (Cat. #IM0505). Unless I'm mistaken, Immunotech is no longer > around but this antibody is/was the best. Any leads on who may actually > have this particular antibody or who may have bought out immunotech would be > much appreciated. > > Thanx in Advance, > > Glen A. Dawson BS, HT & QIHC (ASCP) > Lead IHC Technologist > Milwaukee, WI > > ---------------------------------------------------------------------- Date: 4 Aug 2003 14:01:01 -0500 From: "Yaskovich, Ruth A (NIH/NIDCR)" Subject: RE: Gallyas Stain on Brain I cut mouse brain all the time on plus slides from statlabs with no problem with tissue falling off. Ruth Yaskovich - -----Original Message----- From: Judi Ford [mailto:jlf@jax.org] Sent: Monday, August 04, 2003 1:34 PM To: histonet@pathology.swmed.edu Subject: Gallyas Stain on Brain Hi, I've just finished doing the Gallyas stain for tangles and have found that no matter how gentle I am with the slides tissue falls off the slide during the stain. I thought about using + slides but heard that brain tissue and plus slides don't work due to +/- charges working against each other. Any suggestions? Also, what could I use as a control for tangles? I'm working with mouse tissue. Thanks for your help. Judi Ford Histotechnologist Jackson Laboratory, ME ---------------------------------------------------------------------- Date: 4 Aug 2003 14:01:43 -0500 From: "Wright, Barbara (DNAX)" Subject: RE: MIB-1 (Ki-67) VENDOR Glen, I belive you can get Immunotech products through Becton Dickinson. DAKO's Ki-67 MIB-1 antibody works great too. Hope that helps Barb Wright - -----Original Message----- From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com] Sent: Monday, August 04, 2003 10:38 AM To: histonet@pathology.swmed.edu Subject: MIB-1 (Ki-67) VENDOR All, I am looking for the MIB-1 (Ki-67) antibody that used to be sold by Immunotech (Cat. #IM0505). Unless I'm mistaken, Immunotech is no longer around but this antibody is/was the best. Any leads on who may actually have this particular antibody or who may have bought out immunotech would be much appreciated. Thanx in Advance, Glen A. Dawson BS, HT & QIHC (ASCP) Lead IHC Technologist Milwaukee, WI ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. Here are the messages received yesterday! From ian.montgomery <@t> bio.gla.ac.uk Tue Aug 19 08:23:14 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:21:45 2005 Subject: Fwd: RE: [Histonet] Eosinophils. Message-ID: <5.2.1.1.2.20030819142004.00a0ce70@udcf.gla.ac.uk> Richard, Prefer Carbol Chromotrope or Sirius Red, as do imaging systems. A washed out 5% eosin again does the trick but in this instance I need a spot of IHC. Ian. >Subject: RE: [Histonet] Eosinophils. >Date: Tue, 19 Aug 2003 12:19:07 +0100 >X-MS-Has-Attach: >X-MS-TNEF-Correlator: >Thread-Topic: [Histonet] Eosinophils. >Thread-Index: AcNmOkolOJV3big+RGS08bg/MgnWiwACTuOg >From: "Edwards, R.E." >To: "Ian Montgomery" > > >-----Original Message----- >From: Ian Montgomery [mailto:ian.montgomery@bio.gla.ac.uk] >Sent: 19 August 2003 11:09 >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Eosinophils. > > IHC markers for mouse eosinophils, suggestions, suppliers and > advice welcome. >Ian. > A good H@E???????????????.............. > >Richard Edwards > >MRC TOX UNIT.................LEICESTER................U.K.......... > >Dr. Ian Montgomery, >Histotechnology, >Graham Kerr Building, >Institute of Biomedical & Life Sciences, >University of Glasgow, >Glasgow, >G12 8QQ. >Tel: 0141 339 8855 >Office: 4652 >Lab: 6644. >Pager: 07625 702883 >e-mail: ian.montgomery@bio.gla.ac.uk Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/9f7c32ac/attachment.htm From mlm11 <@t> cornell.edu Tue Aug 19 09:17:57 2003 From: mlm11 <@t> cornell.edu (Mary Lou) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] looking for Alex...... Message-ID: <5.2.1.1.2.20030819101538.02f065d8@postoffice9.mail.cornell.edu> Hi All, Could Alex Molinich contact me please? Thanks, Mary Lou Norman College of Veterinary Medicine Cornell Ithaca From RitaR <@t> lexhealth.org Tue Aug 19 09:24:48 2003 From: RitaR <@t> lexhealth.org (Rita Riddle) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] subscribe Message-ID: _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. 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Thank you, Elina Golder-Novoselsky, Ph.D. -----Original Message----- From: Barnhart, Tammy [mailto:Tbarnhart@primecare.org] Sent: Tuesday, August 19, 2003 4:40 AM To: Histonet (E-mail) Subject: [Histonet] A big thank you Linda, Herb and anyone else who has helped with this list server, I would just like to say thank you for all your hard work with the Histonet. This has become a very valuable tool for the histology community and has helped the field tremendously. We are all forever in your debt. Again, THANK YOU! Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND tbarnhart@primecare.org Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Tue Aug 19 10:53:27 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] New Histonet Listserver IMPORTANT INFORMATION Message-ID: I think both of you are fabulous to keep all of us informed. 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SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/ef28a455/attachment.htm From pontoonsoon <@t> yahoo.com Tue Aug 19 11:19:13 2003 From: pontoonsoon <@t> yahoo.com (Jennifer Rodgers) Date: Fri Sep 16 15:21:45 2005 Subject: [Histonet] (no subject) Message-ID: <20030819161913.28348.qmail@web14506.mail.yahoo.com> Subscibe Jennifer Rodgers --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/f020df40/attachment.htm From akwilliams75 <@t> hotmail.com Tue Aug 19 11:21:28 2003 From: akwilliams75 <@t> hotmail.com (kevin williams) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Borg Decloker, pH 9.5 and options. Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/04dd2438/attachment.htm From brett_connolly <@t> merck.com Tue Aug 19 11:21:33 2003 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] delete Message-ID: <58E3A5F2EFDB224F95D4C62DDDCCA2BE050360D9@uswpmx08.merck.com> Brett M. Connolly, Ph.D. Merck & Co.,Inc. MRL, Imaging Research WP26A-3000 PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-652-2075 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (Whitehouse Station, New Jersey, USA), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD) that may be confidential, proprietary copyrighted and/or legally privileged, and is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please immediately return this by e-mail and then delete it. ------------------------------------------------------------------------------ From cathy <@t> wasatchhisto.com Tue Aug 19 11:37:26 2003 From: cathy <@t> wasatchhisto.com (Cathy Mayton) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Histonet Message-ID: <005601c36670$2d964920$f5693442@selfuggnbwd3wt> Dear Herb or Linda, I guessed I missed something in the past few days. I was subscribed to the digest and have been recently bombarded my histonet emails. I unsubscribed but continue to get bombarded. I just read an email from Linda stating there were some changes made and she referred to a website but did not include it. Can you give me the new information at your convenience? I apologize for being a problem. Take care, Cathy ********************************************************** "Quality Histology with a Personal Touch" A GLP Compliant Laboratory Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 www.wasatchhisto.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/e9afc9bd/attachment.htm From carl.hobbs <@t> kcl.ac.uk Tue Aug 19 11:38:44 2003 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: <006201c36670$5c20ce50$e8345c9f@Carlos> From ryapark <@t> hotmail.com Tue Aug 19 11:46:02 2003 From: ryapark <@t> hotmail.com (ryan park) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: _________________________________________________________________ Help protect your PC: Get a free online virus scan at McAfee.com. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From AMakowsk <@t> med.miami.edu Tue Aug 19 11:54:00 2003 From: AMakowsk <@t> med.miami.edu (Makowski, Anna Lena) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe Message-ID: <3132EBE854CF5B4AA15F9BD2F6781C8D060115EC@medex09.med.miami.edu> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/910c1d8b/attachment.htm From teresajharris <@t> msn.com Tue Aug 19 11:44:45 2003 From: teresajharris <@t> msn.com (teresajharris@msn.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] test Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/5daa8995/attachment.htm From nick.kirk3 <@t> btopenworld.com Tue Aug 19 12:33:41 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:21:46 2005 Subject: FW: [Histonet] Training for Staff Message-ID: Dear Histonetters Due to the rather overwhelming response for copies of my little old training manual (over 2 dozen in 2 days), I have decided that rather than send all those who requested a copy of the old version, it would be more sensible for me to send everyone a copy of the updated version which I am currently working on. Therefore if everyone can wait a week (or maybe a bit longer) I will be more than happy to distribute the all new shiny version in a short while. I just hope I haven't built it up to much and when you get it it's an anticlimax. Meanwhile I shall keep on adding to the list of those who want a copy. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: Nick Kirk [mailto:nick.kirk3@btopenworld.com] Sent: 18 August 2003 22:10 To: David Muskett; Histonet Subject: RE: [Histonet] Training for Staff David Is this a training programme for HPC State Registration? If so, I have produced a 100+ page training manual to be used in conjunction with the Logbook. It's broken down into sections (microtomy, stains, processing etc) and is basically a question and answer book where the trainee can write down answers or draw diagrams to help with their technical training. It does need a bit of updating in places, but your welcome to a copy if you would like one and you can plagiarise it to your hearts content (or throw it in the bin). Let me know and I'll email you a copy. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of David Muskett Sent: 18 August 2003 21:48 To: Histonet Subject: [Histonet] Training for Staff Dear Histonetters I am currently looking to revamp the training programme in my department. Does anyone have any material or old exam questions they are willing to share with me. David Muskett Liverpool, UK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NoraBRL <@t> aol.com Tue Aug 19 12:49:44 2003 From: NoraBRL <@t> aol.com (NoraBRL@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: <29.4660fb5e.2c73bd38@aol.com> From MDiCarlo <@t> KaleidaHealth.Org Tue Aug 19 13:14:41 2003 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] reagent alcohol Message-ID: Histonetters, I have both 95% and absolute ethyl alcohol. A resident gave me some reagent absolute alcohol to use for his project. I need to know if I can interchange reagent with ethanol for processing and staining. Any information will be greatly appreciated. Thanks, Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Hospital CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From tnguyen <@t> mspca.org Tue Aug 19 13:19:06 2003 From: tnguyen <@t> mspca.org (Nguyen, Thao) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe Message-ID: <653D62F4815DD611BD3E00A0C9D178C13DE587@boston.mspca.org> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/fcac3ee5/attachment.htm From garygill <@t> dcla.com Tue Aug 19 13:47:33 2003 From: garygill <@t> dcla.com (Gary Gill) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] reagent alcohol Message-ID: Yes. Reagent alcohol is 90% ethanol, 5% IPA, and 5% methanol. Tissues and staining don't notice the difference. Gary Gill -----Original Message----- From: DiCarlo, Margaret [mailto:MDiCarlo@KaleidaHealth.Org] Sent: Tuesday, August 19, 2003 1:15 PM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] reagent alcohol Histonetters, I have both 95% and absolute ethyl alcohol. A resident gave me some reagent absolute alcohol to use for his project. I need to know if I can interchange reagent with ethanol for processing and staining. Any information will be greatly appreciated. Thanks, Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Hospital CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sharon.willman <@t> bms.com Tue Aug 19 13:52:53 2003 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] catalase Message-ID: <3F427205.33D3F9EC@bms.com> Hi, I was wondering if anyone has a immunohistochemistry staining method for catalase. Any help would be most appreciated. Thanks, Sharon Willman From nick.kirk3 <@t> btopenworld.com Tue Aug 19 13:56:12 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] reagent alcohol In-Reply-To: Message-ID: Peggy We stopped using absolute ethanol many years ago as the extra duty (tax) you had to pay on it made it very expensive for the volumes we used here in the UK. We don't even use a graduated series of alcohols on our processing machines anymore, just straight from Formalin to Industrial methylated spirits (IMS) which is around 99+% ethanol with a bit of methanol in it (means you can't make gin out of it but we all have our sacrifices to bear). We use this on all our routine processing and have never had any problems at all. I know a lot of the labs in the UK use the same protocol. The tissues then go straight into Xylene to clear and then wax. We don't do a lot of bone work, but the femoral heads and marrow trephines we process on it never seem to suffer. Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of DiCarlo, Margaret Sent: 19 August 2003 19:15 To: 'histonet@pathology.swmed.edu' Subject: [Histonet] reagent alcohol Histonetters, I have both 95% and absolute ethyl alcohol. A resident gave me some reagent absolute alcohol to use for his project. I need to know if I can interchange reagent with ethanol for processing and staining. Any information will be greatly appreciated. Thanks, Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Hospital CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lizchlipala <@t> premierhistology.com Tue Aug 19 13:56:49 2003 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Beth Sheppard Message-ID: <000001c36683$a8847db0$74d48a80@LIZ> Looking for Beth Sheppard, if anyone has her e-mail address that would be great Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/edc60e1e/attachment.htm From hborgeri <@t> wfubmc.edu Tue Aug 19 14:04:43 2003 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Beth Sheppard Message-ID: <9AEEF1FB6254224AA355ED285F84916503F8E86A@EXCHVS2.medctr.ad.wfubmc.edu> Beth's e-mail address is sheppard@wfubmc.edu Hermina -----Original Message----- From: Elizabeth Chlipala [mailto:lizchlipala@premierhistology.com] Sent: Tuesday, August 19, 2003 2:57 PM To: 'HistoNet Server' Subject: [Histonet] Beth Sheppard Looking for Beth Sheppard, if anyone has her e-mail address that would be great Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/1adfd12b/attachment.htm From jhatcher <@t> mricordova.com Tue Aug 19 14:05:15 2003 From: jhatcher <@t> mricordova.com (Josh Hatcher) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Unsubscribe Message-ID: <00f701c36684$d35e6180$6529010a@jhatcher> Hey, I hope everything is well with you all. I'm already subscribed to the daily digest, so if you could unsubscribe me for every posting, I'd appreciate it. Thanks. Sincerely, Josh Hatcher Management Recruiters of Cordova, Inc. JHatcher@mricordova.com http://www.MRICordova.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/5b57b9c7/attachment.htm From NoraBRL <@t> aol.com Tue Aug 19 15:51:34 2003 From: NoraBRL <@t> aol.com (NoraBRL@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: <68.33b9f199.2c73e7d6@aol.com> From nick.kirk3 <@t> btopenworld.com Tue Aug 19 15:52:24 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Borg Decloker, pH 9.5 and options. In-Reply-To: Message-ID: Kevin Try looking at this .pdf file for info. http://www.biogenex.com/2003catalog/ca2003sec1.pdf Nick Kirk Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of kevin williams Sent: 19 August 2003 17:21 To: histonet@pathology.swmed.edu Subject: [Histonet] Borg Decloker, pH 9.5 and options. Dear All: I am trying to find a way to make or to procure from someone else a buffer for antigen retreval, we steam. Thanks for your help: Yours faithfully, A. Kevin Williams ---------------------------------------------------------------------------- -- Get MSN 8 and help protect your children with advanced parental controls. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/95c5465e/attachment.htm From laurie.colbert <@t> huntingtonhospital.com Tue Aug 19 16:32:28 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Cryostat Decontamination Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C35@EXCHANGE1.huntingtonhospital.com> According the the NCCLS Publication "Clinical Laboratory Safety; Approved Guidelines" dated Sept. 1996: 1) "Cryostats should be decontaminated frequntly with 70% alcohol after removal off tissue debris." 2) "The cryostat should be defrosted and decontaminated with a tuberculocidal antiseptic at least once a week. If a suspected tissue contains mycobacteria, immediate decontamination should be done with a tuberculocidal antiseptic." Do you all follow both of these requirements? I have always done #1 but not #2. Laurie Colbert Huntington Memorial Hospital Pasadena, CA From gradice <@t> richmond.edu Tue Aug 19 15:33:01 2003 From: gradice <@t> richmond.edu (Gary Radice) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: <54208C08-D284-11D7-9DC8-000393BC23F4@richmond.edu> From WWmn916 <@t> aol.com Tue Aug 19 17:04:02 2003 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Hale's Colloidal Iron Message-ID: <117.27c91b0e.2c73f8d2@aol.com> Greetings Robert, Thankyou so very much for your thorough, complete and prompt response. I'm only sorry I didn't notice such valuable information for the Hales colloidal fe back in May when you posted. But, what a relief now!!!! (It's been one of those unanswered and nagging things that I came up empty handed even after looking through any and all histology books that I have access to.) Today is a good day! Sincerely, Deb King, HT Sacramento, CA P.S. Ellen.....I can't wait to share the information with you too! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/356de8c3/attachment.htm From Marysia33 <@t> aol.com Tue Aug 19 18:10:56 2003 From: Marysia33 <@t> aol.com (Marysia33@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: That movie Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 499 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/a260f157/attachment.bin From CTague <@t> ahs.llumc.edu Tue Aug 19 16:20:41 2003 From: CTague <@t> ahs.llumc.edu (Tague, Curtis) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] manual immunos Message-ID: I read something a while ago about a manual immunostainer or incubator made by a company out of Logan Utah (I think) but can't find the message or the company on a search. It was a slick little machine and I would like to take a look at it again, just for fun. If you are familiar with this product or company, please reply. Thanks. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From JGordon <@t> cellmarque.com Tue Aug 19 16:21:38 2003 From: JGordon <@t> cellmarque.com (Jeff Gordon) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Borg Decloker, pH 9.5 and options. Message-ID: Kevin, I'm not sure which lab you work in, but a popular option may be our Trilogy solution. This EDTA based solution is used by many labs as a universal solution to unmask all their antibodies. It gives high-pH quality unmasking for tougher antibodies like Cyclin D1, ALK-1, TdT, etc....but it isn't high pH so it is less harsh on the tissue than some higher pH solutions. We run all of the antibodies that we offer (with the exception of adenovirus and Her2) with Trilogy antigen retrieval and get optimal staining consistently. Trilogy allows for deparaffinization within the solution while doing HIER, but it doesn't have to be used that way if that is not preferred. It can be used simply as an epitope retrieval solution, just like you would use regular citrate solution. And with no expiration date, no refrigeration, and no diluting needed, it is a very efficient reagent. Add to that that when you purchase the 5 gallon volume (sent in 5 one gallon jugs) it costs less than $80 A GALLON, making it one of, if not THE, most economical reagents on the market. It works in clinical and research settings and has been published in several papers, including from such respected companies as Genentech. We invite you to evaluate it for your IHC needs. If you would like to try it, please e-mail me and I will send you a free sample so that you can see for yourself. BTW, if you want to optimize your staining for some nuclear staining antigens and some antigens that are very sensitive to fixation, like CD10, CD4, CD5, Cyclin D1, TdT, TTF-1, ER, PR, Inhibin, WT1, etc., you many want to consider switching to a pressure cooker, whose high heat tends to really bring out the tougher epitopes to retrieve. We have found that it is fast (15 minutes with no cooldown necessary) and consistent (since it is a closed system with constant 120 degrees centigrade temperature throughout heat retrieval). We have many clients that use Trilogy in the pressure cooker (and many that use it in the steamer as well) for all of their antibodies, even the ones they don't purchase from Cell Marque. If you have any questions, please contact me via e-mail or via phone at 1-800-665-7284, Ext. 12. Jeff Gordon Director of Technical Sales and Marketing Cell Marque Corp. http://www.cellmarque.com -----Original Message----- From: kevin williams [mailto:akwilliams75@hotmail.com] Sent: Tuesday, August 19, 2003 11:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Borg Decloker, pH 9.5 and options. Dear All: I am trying to find a way to make or to procure from someone else a buffer for antigen retreval, we steam. Thanks for your help: Yours faithfully, A. Kevin Williams _____ Get MSN 8 and help protect your children with advanced parental controls. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/b44479ac/attachment.htm From willthon <@t> msn.com Tue Aug 19 17:59:03 2003 From: willthon <@t> msn.com (William Thoendel) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/b4e00809/attachment.htm From willthon <@t> msn.com Tue Aug 19 17:59:26 2003 From: willthon <@t> msn.com (William Thoendel) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe digest Message-ID: -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/8af9a288/attachment.htm From cfarm <@t> sio.midco.net Tue Aug 19 18:58:59 2003 From: cfarm <@t> sio.midco.net (celia) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: <000801c366ad$dc78b760$0100a8c0@youru10ixi0anw> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030819/7a1eed85/attachment.htm From dennijc <@t> vetmed.auburn.edu Wed Aug 20 01:40:58 2003 From: dennijc <@t> vetmed.auburn.edu (dennijc@vetmed.auburn.edu) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Thank you! Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 508 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/97818b5f/attachment.bin From anette.milton <@t> iekf.au.dk Wed Aug 20 00:56:10 2003 From: anette.milton <@t> iekf.au.dk (Anette Milton) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe Message-ID: <002b01c366df$c2dadf90$ae96030a@Fjorgyn> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/24352340/attachment.htm From Marysia33 <@t> aol.com Wed Aug 20 05:27:08 2003 From: Marysia33 <@t> aol.com (Marysia33@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Re: My details Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 494 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/8872b664/attachment.bin From HACKERLAB <@t> aol.com Wed Aug 20 08:04:48 2003 From: HACKERLAB <@t> aol.com (HACKERLAB@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Wicked screensaver Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 496 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/8a103d10/attachment.bin From ccrowder <@t> mail.vetmed.lsu.edu Wed Aug 20 07:19:03 2003 From: ccrowder <@t> mail.vetmed.lsu.edu (Cheryl Crowder) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Subscribe digest Message-ID: Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA ?70803 225-578-9734 FAX: ?225-578-9720 From info <@t> instrumedics.com Wed Aug 20 08:22:46 2003 From: info <@t> instrumedics.com (info@instrumedics.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Approved Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 500 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/c39478c0/attachment.bin From gmacke <@t> shrinenet.org Wed Aug 20 07:28:34 2003 From: gmacke <@t> shrinenet.org (Macke, Gail) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Virus Message-ID: Dear all, Our fire wall has remove three different email from Histonet with viruses in them. Said something about the Thank you virus has been removed from this email. One is from Hackerlabs and two from a Mary both at AOL.com. Just thought I'd let everyone know to fore warn you if your firewall doesn't catch them. All three messages had the "(Histonet)" on them. Gail Macke,HTL Shriners Burns Hospital CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From pmarcum <@t> polysciences.com Wed Aug 20 07:43:40 2003 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Virus In-Reply-To: Message-ID: <002601c36718$aeb35450$4800a8c0@PMARCUM2K> I had a whole collection of them this morning on my incoming mail. I'm not sure where it started but several topics were in the subject line and included mail from several companies, and a number of individuals all through HistoNet. At lest my opportunities to save third world dictators is only coming from other sources. Some days I have up to fifteen of those along with my regular mail. I love HistoNet and the EM server except on these kinds of days. Everyone at HistoNet and the EM Server tries so very hard to avoid these things and it just impossible to do with some of these unauthorized people who only use a name to get on line and send mail. Individuals are much harder to deal with in e-mail. Pam Marcum > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Macke, Gail > Sent: Wednesday, August 20, 2003 8:29 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Virus > > > Dear all, > Our fire wall has remove three different email from Histonet with > viruses in them. Said something about the Thank you virus has been removed > from this email. One is from Hackerlabs and two from a Mary both at > AOL.com. Just thought I'd let everyone know to fore warn you if your > firewall doesn't catch them. All three messages had the "(Histonet)" on > them. > Gail Macke,HTL > Shriners Burns Hospital > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipients. If you are not the intended recipient, (or > authorized to receive for the recipient) you are hereby notified that you > have received this communication in error and that any review, disclosure, > dissemination, distribution or copying of it or its contents is > prohibited. > If you have received this communication in error, please destroy > all copies > of this communication and any attachments and contact the sender by reply > e-mail or telephone (813) 281-0300. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From REEVEML <@t> shands.ufl.edu Wed Aug 20 09:03:51 2003 From: REEVEML <@t> shands.ufl.edu (REEVEML@shands.ufl.edu) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Your details Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 496 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/5d61c655/attachment.bin From latecor <@t> montevideo.com.uy Wed Aug 20 09:42:32 2003 From: latecor <@t> montevideo.com.uy (latecor@montevideo.com.uy) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Approved Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 504 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/9899a4d7/attachment.bin From kgrobert <@t> rci.rutgers.edu Wed Aug 20 09:03:09 2003 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Marine histology Message-ID: <3F437F9D.F1103550@rci.rutgers.edu> Good morning Histonetters- I just got a seahorse from one of our university vets-he wants it processed & stained with H& E. It was fixed in Formalde-fresh buffered formalin for nine days, and it needs to be decalcified. Would anybody happen to know how long it would take a seahorse to decalcify? I've done fish before, but not seahorses. Any advice on processing would be welcome as well. (Yes, I know about the book Dr. Callis wrote on animal tissue processing; it hasn't gotten here yet! :o) Thanks for all your help- Kathleen Roberts Principal Lab Technician Molecular Pathology Core Facility Dept of Pharmacology & Toxicology Ernest Mario School of Pharmacy Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 From HornHV <@t> archildrens.org Wed Aug 20 09:02:56 2003 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Virus Message-ID: Me tooo..................AND they are saying they are coming from me!!! I never sent them!!! Hazel Horn, HT/HTL (ASCP) Histology Supervisor Arkansas Children's Hospital Phone - 501.364.4240 Fax - 501.364.3912 -----Original Message----- From: Pamela Marcum [mailto:pmarcum@polysciences.com] Sent: Wednesday, August 20, 2003 7:44 AM To: Macke, Gail; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Virus I had a whole collection of them this morning on my incoming mail. I'm not sure where it started but several topics were in the subject line and included mail from several companies, and a number of individuals all through HistoNet. At lest my opportunities to save third world dictators is only coming from other sources. Some days I have up to fifteen of those along with my regular mail. I love HistoNet and the EM server except on these kinds of days. Everyone at HistoNet and the EM Server tries so very hard to avoid these things and it just impossible to do with some of these unauthorized people who only use a name to get on line and send mail. Individuals are much harder to deal with in e-mail. Pam Marcum > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Macke, > Gail > Sent: Wednesday, August 20, 2003 8:29 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Virus > > > Dear all, > Our fire wall has remove three different email from Histonet with > viruses in them. Said something about the Thank you virus has been > removed from this email. One is from Hackerlabs and two from a Mary > both at AOL.com. Just thought I'd let everyone know to fore warn you > if your firewall doesn't catch them. All three messages had the > "(Histonet)" on them. > Gail Macke,HTL > Shriners Burns Hospital > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments > may contain confidential and privileged information for the use of the > designated recipients. If you are not the intended recipient, (or > authorized to receive for the recipient) you are hereby notified that > you have received this communication in error and that any review, > disclosure, dissemination, distribution or copying of it or its > contents is prohibited. If you have received this communication in > error, please destroy all copies > of this communication and any attachments and contact the sender by reply > e-mail or telephone (813) 281-0300. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital From icool48202 <@t> yahoo.com Wed Aug 20 09:07:41 2003 From: icool48202 <@t> yahoo.com (sanjay rakhade) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe Message-ID: <20030820140741.46313.qmail@web14701.mail.yahoo.com> --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/17b6420f/attachment.htm From bill501 <@t> mindspring.com Wed Aug 20 10:13:57 2003 From: bill501 <@t> mindspring.com (bill501@mindspring.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: Your application Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 500 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/6c452fdd/attachment.bin From jcox90 <@t> yahoo.com Wed Aug 20 09:17:11 2003 From: jcox90 <@t> yahoo.com (jill cox) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Attachments Message-ID: <20030820141711.25980.qmail@web40903.mail.yahoo.com> Hello, I am getting nothing but attachments with no message!! Is anyone else receiving this? Jill Cox HT (ASCP) Seattle Histology Lab --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/0d6baf3e/attachment.htm From SADAMS <@t> HCMHCARES.ORG Wed Aug 20 09:38:44 2003 From: SADAMS <@t> HCMHCARES.ORG (Adams, Sharon K.) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] RE: unsubscribe Message-ID: <684DF8CDE1FB6A428499DA65D753D12224AC52@jupiter.hcmh.org> -----Original Message----- From: HistoNet Server [mailto:histonet@pathology.swmed.edu] Sent: Tuesday, August 12, 2003 15:15 To: Adams, Sharon K. Subject: re: subscribe Your address has been added to the addresses that comprise this Listserv List. Welcome to HISTONET. This is an electronic mailing list for the exchange of information pertaining to histotechnology and related fields. PLEASE SAVE THIS MESSAGE. It contains useful information about how to use the list and what to do if you experience problems. It also includes some basic rules for email etiquette (Netiquette) which will be helpful to those who are new to this form of communication. WHAT IS A LISTSERVER? A list server is a computer that runs software which will receive incoming electronic mail (email) messages and reroute them automatically to everyone on the subscriber list. Email uses the vast expanse of the Internet to allow almost instantaneous communication between networked computers around the world. Our system uses the LISTSTAR software from Quarterdeck Corporation (California) and can currently send about 30 messages a minute. With the present number of subscribers, we are processing about 10,000 outbound messages a day. WHO SHOULD SUBSCRIBE? Anyone interested in research or clinical applications of histology, immunohistochemistry, in-situ hybridization pathology, and electron microscopy may find Histonet informative and useful. Currently, there are more than 850 subscribers from all over the world. Subscribers include hospital employees from major urban centers and obscure remote locales, university researchers, botanists and the employees of commercial laboratories, government agencies, veterinary facilities and a wide variety of commercial industrial ventures. WHO RUNS HISTONET? The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using hardware and software owned by the University of Texas Southwestern Medical School, Department of Pathology in Dallas, Texas. If you have any questions or problems with Histonet please contact Linda Margraf at LMargraf@childmed.dallas.tx.us. HOW DOES THE LIST WORK? This server, unlike many systems, uses ONLY ONE ADDRESS to send commands to the computer and to post messages. The server will recognize commands sent in the SUBJECT line of the message and only when they are spelled exactly as listed below. Anything not identified as a command will be circulated to EVERYONE on the list. The following is a list of commands the server recognizes: subscribe Your address will be added to the list of subscribers. You will then be able to send messages to this list that will be forwarded to all other list subscribers. You will begin to receive all messages sent to the list by other subscribers. subscribe digest Your address will be added to the list of subscribers who receive a digest instead of each forwarded message. A digest is a compilation of all the messages received in a 24 hour period. It is sent to the digest subscribers every night after midnight. Digest subscribers can post and respond to messages the same as "real-time" subscribers. digests A list of available digests will be returned to you. Histonet stores old messages as daily digests for approximately three months. To read previous messages, copy the list of available digests, mark the dates of interest and return it to the server. unsubscribe Your address will be removed from the list of subscribers. You will no longer be able to send messages to the members of the list. help A list of the commands recognized by the server will be returned to you. WHAT ARE THE RULES? You may post any questions you wish pertaining to histology, pathology, in-situ hybridization, immunohistochemistry etc. Equipment and reagent evaluations, laboratory management issues, government regulations, and job opportunities are all appropriate topics. The University asks that we restrict the use of its hardware and software to business purposes only (occasional jokes do slip through but PLEASE use restraint). Vendors and those with commercial interests in histology products are welcome contributors however, we ask that blatant advertisements be avoided at all times. It is fine to refer to product that your company produces if it is pertinent to a topic being discussed on the list. Unsolicited advertisements are poorly tolerated by the members and you will likely receive a number of negative comments if you overstep the boundaries. Please contact Linda Margraf at LMargraf@childmed.dallas.tx.us if you are not sure about the appropriateness if a message you wish to post. BASIC HISTONET "NETIQUETTE" It is most helpful to the list members if you post your responses to queries to everyone on the list and not just as a personal reply to the person asking the question. That way duplicate messages are minimized and we all learn from each other's comments. Likewise, if you post a question and get a number of responses back directly to you, it is helpful to everyone if you could send out a summary of the replies you got to Histonet. Please avoid abbreviations unless they are explained in your message. For example: immunohistochemistry (IHC). This list circulates to a wide variety of individuals and what seems obvious to you may have no meaning on the other side of the world. Please sign your letter and include your institution or affiliation and location. Not all email systems have headers which identify the sender. Do use the subject line to indicate the topic of your message. DON'T USE ONLY CAPITAL LETTERS -it is considered shouting. Please send questions and problems about the list directly to Linda Margraf at LMargraf@childmed.dallas.tx.us and don't circulate them to the >850 subscribers on the list. Be careful when sending commands to the server to put the command in the SUBJECT LINE and spell it correctly. Please do not send images as attachments with your message. We can now post images at our web site (http://pathcuri1.swmed.edu). To have an image posted send it to Herb Hagler at herb.hagler@email.swmed.edu. es From Marysia33 <@t> aol.com Wed Aug 20 10:45:38 2003 From: Marysia33 <@t> aol.com (Marysia33@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Re: That movie Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 496 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/96ba9bba/attachment.bin From kgrobert <@t> rci.rutgers.edu Wed Aug 20 10:05:16 2003 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Marine histology References: <3F437F9D.F1103550@rci.rutgers.edu> Message-ID: <3F438E2B.4BBAC417@rci.rutgers.edu> Never mind-it turned out that this is a baby seahorse, quite small (I originally thought it was one of the big ones, split into several cassettes)-he won't take long at all to decalcify, and we figure that we can process it the same way as we do the rest of our samples. Thanks anyway- Kathleen Kathleen Roberts wrote: > Good morning Histonetters- > > I just got a seahorse from one of our university vets-he wants it > processed & stained with H& E. It was fixed in Formalde-fresh buffered > formalin for nine days, and it needs to be decalcified. Would anybody > happen to know how long it would take a seahorse to decalcify? I've > done fish before, but not seahorses. Any advice on processing would be > welcome as well. > > (Yes, I know about the book Dr. Callis wrote on animal tissue > processing; it hasn't gotten here yet! :o) > > Thanks for all your help- > Kathleen Roberts > Principal Lab Technician > Molecular Pathology Core Facility > Dept of Pharmacology & Toxicology > Ernest Mario School of Pharmacy > Rutgers University > 41 B Gordon Rd > Piscataway, NJ 08854 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Aug 20 09:53:55 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Virus In-Reply-To: <002601c36718$aeb35450$4800a8c0@PMARCUM2K> References: <002601c36718$aeb35450$4800a8c0@PMARCUM2K> Message-ID: It is of course more complex than this. Most of these recent viruses and worms harvest email addresses from your personal PC and use them to get into listserves. Today I got a bunch of bounces and notices from Yahoo groups and Histonet that it had removed a virus coming from my email address. These are as annoying as Spam. As a Mac user I can't spread the virus, but they can make them appear to come from me. Bill At 8:43 AM -0400 8/20/03, Pamela Marcum wrote: >HistoNet and the EM Server tries so very >hard to avoid these things and it just impossible to do with some of these >unauthorized people who only use a name to get on line and send mail. >Individuals are much harder to deal with in e-mail. Pam Marcum -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) From lsb <@t> jax.org Wed Aug 20 09:55:30 2003 From: lsb <@t> jax.org (Lesley S. Bechtold) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Reference Labs Message-ID: <5.1.0.14.0.20030820105420.00b11640@aretha.jax.org> Hi Everyone, I'm looking for names of Histology Reference Labs. The only one I'm familiar with is Idexx. If anyone knows of any, I'd like to hear. Thanks! Lesley Lesley S. Bechtold Supervisor, Biological Imaging The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6191 From Terry.Marshall <@t> rothgen.nhs.uk Wed Aug 20 09:58:56 2003 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] New virus - appears to be "sobig" Message-ID: Virus Profile Virus Information Name: W32/Sobig.f@MM Risk Assessment - Home Users: High - Corporate Users: Medium Date Discovered: 8/18/2003 Date Added: 8/18/2003 Origin: Unknown Length: approx 72,568 Bytes Type: Virus SubType: Internet Worm DAT Required: 4287 Quick Links Virus Characteristics Indications of Infection Method of Infection Removal Instructions Aliases Buy or Update New Users Get Protected Now: Buy VirusScan Online Update VirusScan Online Virus Characteristics This detection is for a new variant of W32/Sobig. In common with previous variants, the worm is written in MSVC, and bears the following characteristics: propagates via email, constructing outgoing messages with its own SMTP engine propagates over network shares (not confirmed in testing yet) Note: The worm carries garbage data appended to end of file, so exact filesize and file checksum may vary. Installation The worm copies itself onto the victim machine as WINPPR32.EXE into %Windir%, for example: C:\WINNT\WINPPR32.EXE A configuration file is also dropped to %Windir%: C:\WINNT\WINSTT32.DAT The following Registry keys are added to hook system startup: HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\Windows\CurrentVersion\Run "TrayX" = %Windir%\WINPPR32.EXE /sinc HKEY_CURRENT_USER\SOFTWARE\Microsoft\Windows\CurrentVersion\Run "TrayX" = %Windir%\WINPPR32.EXE /sinc Mail Propagation The worm mails itself to email addresses harvested from the victim machine, using its own SMTP engine to construct outgoing messages. Target email addresses are harvested from files with the following extensions: DBX HLP MHT WAB EML TXT HTM HTML Outgoing messages are constructed as follows: Subject: Your details Thank you! Re: Thank you! Re: Details Re: Re: My details Re: Approved Re: Your application Re: Wicked screensaver Re: That movie Attachment: your_document.pif document_all.pif thank_you.pif <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< the one we got(Terry) your_details.pif details.pif document_9446.pif application.pif wicked_scr.scr movie0045.pif Body: See the attached file for details Please see the attached file for details The "From:" address may be spoofed with an address extracted from the victim machine. Therefore the perceived sender is most likely not a pointer to the infected user. Contacting Remote NTP Servers The worm contains a list of IP addresses for remote NTP servers, to which it sends NTP packets (destination port 123). Self-Termination In common with previous W32/Sobig variants, this variant contains a date triggered self-termination routine. If the date is September 10th 2003 or later, the worm will no longer propagate. Indications of Infection Existence of the WINPPR32.EXE file in %WinDir% Existence of the Registry hooks detailed above Unexpected NTP traffic to remote servers Method of Infection This worm propagates via email (contains its own SMTP engine) and over network shares. Removal Instructions DAT Files Detection is included in the 4287 DAT files. In addition to the DAT version requirements for detection, the specified engine version (or greater) must also be used. Alternatively, the following EXTRA.DAT packages are available (HOW TO use an EXTRA.DAT).. EXTRA.DAT - should be extracted to the same directory where CLEAN.DAT, NAMES.DAT, and SCAN.DAT are (typically C:\Program Files\Common Files\Network Associates\VirusScan Engine\4.0.xx) or SUPER EXTRA.DAT - EXTRA.DAT self installer Stand Alone Remover Stinger has been updated to include detection/removal of this threat. Manual Removal Instructions To remove this virus "by hand", follow these steps: - Win9x/ME - Reboot the system into Safe Mode (hit the F8 key as soon as the Starting Windows text is displayed, choose Safe Mode. - WinNT/2K/XP - Terminate the process WINPPR32.EXE Delete the following files from your WINDOWS directory (typically c:\windows or c:\winnt) WINPPR32.EXE WINSTT32.DAT Edit the registry Delete the "TrayX" value from HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\ Windows\CurrentVersion\Run HKEY_CURRENT_USERS\SOFTWARE\Microsoft\ Windows\CurrentVersion\Run Additional Windows ME/XP removal considerations Aliases W32.Sobig.F@mm (NAV), WORM_SOBIG.F (Trend) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From Diane.Gladney <@t> se.amedd.army.mil Wed Aug 20 10:07:21 2003 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Reference Labs Message-ID: <9D41AB7C56F8304F98537ABD87B249F6626117@dasmthgbz001.amedd.army.mil> Quest Diagnostics has a histology department. We have inquired about their services in case we must need to use them when I am on leave or our pathologist is on a long vacation or at a conference. I suggest looking on the internet for the lab nearest you. We are already using Quest for ER/PR analysis and Her-2-Neu. Hope this helps. Diane Diane C. Gladney, HT (ASCP) Histology /Cytology Supervisor Moncrief Army Community Hospital P.O. BOX 484 4500 Stuart Ave. FT. Jackson, SC 29207 (803) 751-2530 DSN 734-2530 EMAIL: diane.gladney@se.amedd.army.mil OR dcgx1@aol.com -----Original Message----- From: Lesley S. Bechtold [mailto:lsb@jax.org] Sent: Wednesday, August 20, 2003 10:56 AM To: histonet@Pathology.swmed.edu Subject: [Histonet] Reference Labs Hi Everyone, I'm looking for names of Histology Reference Labs. The only one I'm familiar with is Idexx. If anyone knows of any, I'd like to hear. Thanks! Lesley Lesley S. Bechtold Supervisor, Biological Imaging The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6191 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Aug 20 10:18:55 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] New virus - appears to be "sobig" Message-ID: I've received 100 of these from histonet with the subject line just as you describe! Thanks for the information. Jacqueline M. O'Connor HT(ASCP) Abbott Laboratories Global Pharmaceutical Research and Development Discovery Chemotheraputics 847.938.4919 Fax 847.938.3266 "Marshall Terry Dr, Consultant Histopathologist" Sent by: histonet-admin@lists.utsouthwestern.edu 08/20/2003 09:58 AM To: "Adams, Sharon K." , "HistoNet Server" cc: Subject: [Histonet] New virus - appears to be "sobig" Virus Profile Virus Information Name: W32/Sobig.f@MM Risk Assessment - Home Users: High - Corporate Users: Medium Date Discovered: 8/18/2003 Date Added: 8/18/2003 Origin: Unknown Length: approx 72,568 Bytes Type: Virus SubType: Internet Worm DAT Required: 4287 Quick Links Virus Characteristics Indications of Infection Method of Infection Removal Instructions Aliases Buy or Update New Users Get Protected Now: Buy VirusScan Online Update VirusScan Online Virus Characteristics This detection is for a new variant of W32/Sobig. In common with previous variants, the worm is written in MSVC, and bears the following characteristics: propagates via email, constructing outgoing messages with its own SMTP engine propagates over network shares (not confirmed in testing yet) Note: The worm carries garbage data appended to end of file, so exact filesize and file checksum may vary. Installation The worm copies itself onto the victim machine as WINPPR32.EXE into %Windir%, for example: C:\WINNT\WINPPR32.EXE A configuration file is also dropped to %Windir%: C:\WINNT\WINSTT32.DAT The following Registry keys are added to hook system startup: HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\Windows\CurrentVersion\Run "TrayX" = %Windir%\WINPPR32.EXE /sinc HKEY_CURRENT_USER\SOFTWARE\Microsoft\Windows\CurrentVersion\Run "TrayX" = %Windir%\WINPPR32.EXE /sinc Mail Propagation The worm mails itself to email addresses harvested from the victim machine, using its own SMTP engine to construct outgoing messages. Target email addresses are harvested from files with the following extensions: DBX HLP MHT WAB EML TXT HTM HTML Outgoing messages are constructed as follows: Subject: Your details Thank you! Re: Thank you! Re: Details Re: Re: My details Re: Approved Re: Your application Re: Wicked screensaver Re: That movie Attachment: your_document.pif document_all.pif thank_you.pif <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< the one we got(Terry) your_details.pif details.pif document_9446.pif application.pif wicked_scr.scr movie0045.pif Body: See the attached file for details Please see the attached file for details The "From:" address may be spoofed with an address extracted from the victim machine. Therefore the perceived sender is most likely not a pointer to the infected user. Contacting Remote NTP Servers The worm contains a list of IP addresses for remote NTP servers, to which it sends NTP packets (destination port 123). Self-Termination In common with previous W32/Sobig variants, this variant contains a date triggered self-termination routine. If the date is September 10th 2003 or later, the worm will no longer propagate. Indications of Infection Existence of the WINPPR32.EXE file in %WinDir% Existence of the Registry hooks detailed above Unexpected NTP traffic to remote servers Method of Infection This worm propagates via email (contains its own SMTP engine) and over network shares. Removal Instructions DAT Files Detection is included in the 4287 DAT files. In addition to the DAT version requirements for detection, the specified engine version (or greater) must also be used. Alternatively, the following EXTRA.DAT packages are available (HOW TO use an EXTRA.DAT).. EXTRA.DAT - should be extracted to the same directory where CLEAN.DAT, NAMES.DAT, and SCAN.DAT are (typically C:\Program Files\Common Files\Network Associates\VirusScan Engine\4.0.xx) or SUPER EXTRA.DAT - EXTRA.DAT self installer Stand Alone Remover Stinger has been updated to include detection/removal of this threat. Manual Removal Instructions To remove this virus "by hand", follow these steps: - Win9x/ME - Reboot the system into Safe Mode (hit the F8 key as soon as the Starting Windows text is displayed, choose Safe Mode. - WinNT/2K/XP - Terminate the process WINPPR32.EXE Delete the following files from your WINDOWS directory (typically c:\windows or c:\winnt) WINPPR32.EXE WINSTT32.DAT Edit the registry Delete the "TrayX" value from HKEY_LOCAL_MACHINE\SOFTWARE\Microsoft\ Windows\CurrentVersion\Run HKEY_CURRENT_USERS\SOFTWARE\Microsoft\ Windows\CurrentVersion\Run Additional Windows ME/XP removal considerations Aliases W32.Sobig.F@mm (NAV), WORM_SOBIG.F (Trend) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/8d5fb66a/attachment.htm From peoshel <@t> wisc.edu Wed Aug 20 10:31:16 2003 From: peoshel <@t> wisc.edu (Philip Oshel) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Attachments In-Reply-To: <20030820141711.25980.qmail@web40903.mail.yahoo.com> References: <20030820141711.25980.qmail@web40903.mail.yahoo.com> Message-ID: Someone's -- or more than one person's -- computer is infected with the latest virus and is sending the stuff, mostly spoofing email addresses from the computer's address book, so the mail looks like it's from a legitimate source. My address has been spoofed this way. Delete all the attachments *without opening them* and the emails. Some worms and viruses can use header vulnerabilities in MIME-compliant programs like Outlook, Outlook Express, Eudora, etc.. Run a virus program on your computer to make sure that you're not infected. Also, if you're using Windows on your computer, check with your IT people to make sure you've got the latest patch to fix yet another of MS's security flaws (self-inflicted, most of them). It might have come through your Yahoo account -- web-based email accounts are easier targets for viruses and worms than most other accounts. Phil Hello, I am getting nothing but attachments with no message!! Is anyone else receiving this? Jill Cox HT (ASCP) Seattle Histology Lab Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -- Philip Oshel Supervisor, BBPIC microscopy facility Department of Animal Sciences University of Wisconsin 1675 Observatory Drive Madison, WI 53706 - 1284 voice: (608) 263-4162 fax: (608) 262-5157 (dept. fax) From Linke_Noelle <@t> Allergan.com Wed Aug 20 10:57:54 2003 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] catalase & peroxisome proliferator receptor Message-ID: <805C4A052A253B4A8E4948B13F54D12704D176@IRMAIL102.irvine.allergan.com> Is anyone using these antibodies on formalin fixed paraffin embedded tissues? If so, can you recommend vendor(s)? Thank you!! Noelle Linke, BS, HTL(ASCP) Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/095c6868/attachment.htm From conmac <@t> cc.usu.edu Wed Aug 20 11:37:01 2003 From: conmac <@t> cc.usu.edu (Connie McManus) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] test In-Reply-To: Message-ID: <003201c36739$48b31ac0$4a737b81@usu.edu> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/5f042247/attachment.htm From gcallis <@t> montana.edu Wed Aug 20 11:57:10 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Scytek manual immunostainer from Logan Utah Message-ID: <3.0.6.32.20030820105710.00b78728@gemini.msu.montana.edu> The device is called Slidemaster. It holds 20 slides, can be put into an incubator, refrigerator, or on a rotator for more agitation, and it can be stacked. We cover with a black towel for immunofluorescent staining (something that cannot be done on an open to room light automated stainer!) We have 7 of them in our lab, and have set up 5 at a time to stain 100 slides manually (yes, possible!), finishing after lunch, one person doing the work. Go Scytek website for more information on the device. It costs approx $400 but considering the price of automation, cheap! and they take up very little space. The joy is absolute versatility to use whatever reagents one desires and in research situation has proven to be a workhorse incubation chamber in our lab. Suddenly, IHC is very popular when done in my lab using Slidemaster (also called the "Flat Thing"! If this is a sales pitch, No! I get no commission from Scytek, just a satisfied customer speaking up. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu From ander093 <@t> gold.tc.umn.edu Wed Aug 20 12:22:43 2003 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] test....sorry In-Reply-To: <3.0.6.32.20030820105710.00b78728@gemini.msu.montana.edu> Message-ID: <5.2.0.9.0.20030820122212.009f67e0@ander093.email.umn.edu> From alust1 <@t> hotmail.com Wed Aug 20 12:35:16 2003 From: alust1 <@t> hotmail.com (Andrew Lust) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] tissues for a positive control for collagen type III Message-ID: Does anybody have any ideas of tissues that I could section that collagen type III? thanks again Andrew _________________________________________________________________ Protect your PC - get McAfee.com VirusScan Online http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From jlinda <@t> ces.clemson.edu Wed Aug 20 13:03:16 2003 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Worst week for computer virus! Message-ID: <5.1.0.14.2.20030820135314.00a96130@mailhost.ces.clemson.edu> Dear HistoNetters, Having spent all morning eradicating the "So Big" virus and "Stinging" my PC to clean it, I just wanted to share some info with you. DO NOT BLAME THE HISTONET OR YOUR COLLEAGUES FOR THE VIRUS. Stupid me...I thought a vendor had sent me a new movie of their equipment and tried to open the movie! Bad mistake:-( Anyhow, here is the web address of a site that should be helpful: http://antivirus.about.com/library/weekly/aa081903a.htm Part of the article is as follows: Tuesday, August 19, 2003: A new SoBig variant has sprung forth, sending huge volumes of infected email to thousands of unlucky recipients. Like its predecessor, SoBig.E, SoBig.F sifts through files on infected users' drives to obtain email addresses which in turn are sent the worm. SoBig.F also has the same insidious twist found with SoBig.E. The worm sends itself as if "From" one of the addresses found on the infected users' system. This not only leaves an innocent party dealing with angry email accusing them of sending the worm, but they also must contend with "out of office" replies and undeliverable notifications for email they never sent. Sorin Dudea, Head of Virus Research at BitDefender says he has never seen such fast spreading in such short time: I have colleagues in the commercial team that have already received thousands of infected e-mails and they just keep receiving them", Sorin concluded. In his technical paper, Sobig.e - Evolution of the Worm, Joe analyzed the workings of the various variants of SoBig, noting the worm's primary goal was "to create a massive network of anonymous proxy servers for the purpose of spam." He further speculates, "This is likely a financial endeavor for the author alone or perhaps in concert with a gang of criminals, supporting themselves through spamming, identity theft and bank fraud. Joe attributes this newest release of SoBig.F to the Blaster worm, noting, "the Blaster worm caused many people to install antivirus software which detected and removed previous Sobig infections as well. This probably severely diminished the number of proxy servers installed by the Sobig worm, forcing the author to re-release to keep the numbers up." Hope this is helpful! Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From laurie.colbert <@t> huntingtonhospital.com Wed Aug 20 14:03:49 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] cryostat decontamination Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628001C5BB5E@EXCHANGE1.huntingtonhospital.com> How do others clean the cryostat after a known or suspected TB case? Also, do you decontaminate once a week with a tuberculocidal antiseptic? Laurie Colbert Huntington Memorial Hospital From DRG <@t> Stowers-Institute.org Wed Aug 20 14:26:48 2003 From: DRG <@t> Stowers-Institute.org (Grant, Debra) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] gelatin/sucrose for cryo Message-ID: Hi all, Has anyone prepared mouse embryo for cryo sectioning in gelatin/15%sucrose? All answers in this area would be greatly appreciated. Thanks in advance! Debby Grant Research Technician II Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 drg@stowers-institute.org From plucas <@t> biopath.org Wed Aug 20 14:44:02 2003 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] test Message-ID: <019201c36753$685763d0$8a176b43@new001> test -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/e43df8be/attachment.htm From KHays <@t> mbhs.org Wed Aug 20 15:20:23 2003 From: KHays <@t> mbhs.org (KHays@mbhs.org) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] rapid oil red o stain Message-ID: Kathy Tedford-Hays HT (ASCP) Technical Specialist, Histology Dept (601)-968-3070 ext 7398 Baptist Medical Center 1225 North State Street Jackson, MS 39202 anyone in histoland know of a rapid oil red o stain on frozen section and paraffin sections? The information contained in this email is confidential and is intended for the recipient only. Do not forward this email without permission from its originator. From ThisisAnn <@t> aol.com Wed Aug 20 15:27:30 2003 From: ThisisAnn <@t> aol.com (ThisisAnn@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] mail Message-ID: <36.466aacf5.2c7533b2@aol.com> Why am I getting all of this histonet mail, please make it stop! Thank you -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/cb0d759e/attachment.htm From gliuygao <@t> hotmail.com Wed Aug 20 15:39:13 2003 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] humidity Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/96c157a3/attachment.htm From drelliott0 <@t> lycos.com Wed Aug 20 15:29:37 2003 From: drelliott0 <@t> lycos.com (Elliott Grammon) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Antibody Processing Message-ID: Dear Histonetters, I have had numerous inquiries as to whether orders for Novocastra antibodies must now be placed directly with Novocastra in the UK since my last message. The answer is "NO!" The distributor for Novocastra antibodies in the USA and Canada is VISION BioSystems (Norwell, MA). After all, they bought Novocastra Labs in 2002 - what better place to get NCL antibodies? I will be entering them into our system as a vendor for future purchases. I hope this answers many of your questions. Elliott G. Osterhaus MD PhD ____________________________________________________________ Get advanced SPAM filtering on Webmail or POP Mail ... Get Lycos Mail! http://login.mail.lycos.com/r/referral?aid=27005 From dellav <@t> musc.edu Wed Aug 20 16:10:41 2003 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Marine histology Message-ID: I recommend that you contact Marie Wright at the Gulf Coast Research Laboratories of the Univ of Mississippi. She has extensive experience with crustaceans. marie.wright@usm.edu Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> Kathleen Roberts 08/20/03 10:03AM >>> Good morning Histonetters- I just got a seahorse from one of our university vets-he wants it processed & stained with H& E. It was fixed in Formalde-fresh buffered formalin for nine days, and it needs to be decalcified. Would anybody happen to know how long it would take a seahorse to decalcify? I've done fish before, but not seahorses. Any advice on processing would be welcome as well. (Yes, I know about the book Dr. Callis wrote on animal tissue processing; it hasn't gotten here yet! :o) Thanks for all your help- Kathleen Roberts Principal Lab Technician Molecular Pathology Core Facility Dept of Pharmacology & Toxicology Ernest Mario School of Pharmacy Rutgers University 41 B Gordon Rd Piscataway, NJ 08854 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bills <@t> icpmr.wsahs.nsw.gov.au Wed Aug 20 16:56:33 2003 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] cryostat decontamination References: <0BE6ADFAE4E7E04496BF21ABD346628001C5BB5E@EXCHANGE1.huntingtonhospital.com> Message-ID: <004101c36765$ebf0f1e0$7a6e080a@wsahs.nsw.gov.au> Laura, We have a protocol for decontaminating all our Cryostats once per week. We have 2 Shandon 660E models which have the heating block which you fill with 40% formadlehyde then turn on the decontamination cycle. This is performed overnight Friday. After this process the shavings are removed using tissue damped with 70% ethanol. The others have a formaldehyde "bomb" prepared and placed into the chamber, then left sealed to defrost for 24 hours. These are then cleaned using 70% ethanol. The same procedures are carried out if we have cut anything suspected of being an infective agent, immediately after the case is finished. We have the luxury of two cryostats available in our main laboratory. All the best Bill Sinai Laboratory Manager Tissue Pathology ICPMR P.O. Box 533 Wentworthville NSW 2145 pH 02 9845 7774 ----- Original Message ----- From: "Laurie Colbert" To: Sent: Thursday, August 21, 2003 5:03 AM Subject: [Histonet] cryostat decontamination How do others clean the cryostat after a known or suspected TB case? Also, do you decontaminate once a week with a tuberculocidal antiseptic? Laurie Colbert Huntington Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From laurie.colbert <@t> huntingtonhospital.com Wed Aug 20 17:53:22 2003 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] humidity Message-ID: <0BE6ADFAE4E7E04496BF21ABD34662801D0C39@EXCHANGE1.huntingtonhospital.com> I use xylene recycling pads from Creative Waste on my H&E stainer. There are different size pads you can order to fit the containers of different stainers. The pads contains little beads (which I assume are some sort of dessicant) that remove stain, alcohol, and water from the xylene. We change the xylenes once a week (whereas we were changing them daily previously). I was told we could go much longer without changing, but I'm being conservative for now. We will probably increase the time between changes. I've been using these pads for about a month and we've had no problems. I am not associated with Creative Waste in any way, and if you would like to contact them their number is (888) 795-8300. They are located in Oregon. Laurie Colbert Huntington Memorial Hospital Pasadena, CA -----Original Message----- From: yan gao [mailto:gliuygao@hotmail.com] Sent: Wednesday, August 20, 2003 1:39 PM To: histonet@pathology.swmed.edu Subject: [Histonet] humidity A few weeks a ago, Histonet has been discussed humidity cause smear or bloody eosin stain. Somebody suggested leaving a mesh or stone in alcohol and xylene can avoid the bloody eosin stain. Can anyone tell me again where can I get this mesh? Thanks a lot. Yan Gao Toxikon _____ Get MSN 8 and enjoy automatic e-mail virus protection. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/27308944/attachment.htm From akwilliams75 <@t> hotmail.com Wed Aug 20 19:50:11 2003 From: akwilliams75 <@t> hotmail.com (kevin williams) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] High Functioning Autistic Histologists Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/bea392a9/attachment.htm From ihctech2000 <@t> yahoo.com Wed Aug 20 21:24:47 2003 From: ihctech2000 <@t> yahoo.com (Sun Zhon) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] autofluorescence in frozen tissues Message-ID: <20030821022447.79774.qmail@web80703.mail.yahoo.com> Dear Histonetters, Can anyone tell me, in general what kind of frozen tissues autofluorescence? How do I get rid of the autofluorescence before I do my IF? Thank you very much for your help. Sun --------------------------------- Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030820/1a7ab948/attachment.htm From malcolm_gillham <@t> health.qld.gov.au Wed Aug 20 21:49:28 2003 From: malcolm_gillham <@t> health.qld.gov.au (Malcolm Gillham) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsuscribe Message-ID: From jluis.palazon <@t> icman.csic.es Thu Aug 21 02:59:07 2003 From: jluis.palazon <@t> icman.csic.es (Jose Luis Palazon Fernandez) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] sudan black B Message-ID: <20030821075907.BCBC1213F0@perceval.uca.es> Dear List members. I need your advice I stained some slides with sudan black B. I used 4 slides per specimen and I did (delipidized, sudan black B, Bromide-sudan black, and bromide-acetone-sudan black). I have noticed that the slides were clearly black after staining, the bromide treated the most black, but after 1-2 weeks, they are turning green in color. Is the color of sudan black B stained slides not permanent? must the slides be read inmediately? or do this change in color alter the results if I read some recently stained slides and other stained some weeks ago?. Is it possible (adequate) to put the coverlips out and re-stain the slides? I would appreciate some advice from you. Thanks in advance José Luis From t.hacker <@t> har.mrc.ac.uk Thu Aug 21 06:09:02 2003 From: t.hacker <@t> har.mrc.ac.uk (Terry Hacker) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] unsubscribe Message-ID: <001001c367d4$a0c09850$d8f1f682@A383R28DELL360> Terry Hacker Head of Histology and Electron Microscopy Services Medical Research Council Harwell Didcot Oxfordshire OX11 ORD Tel: 01235 841128 Fax: 01235 841200 e-mail t.hacker@har.mrc.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/dcd15e98/attachment.htm From Histopatty <@t> aol.com Thu Aug 21 07:04:38 2003 From: Histopatty <@t> aol.com (Histopatty@aol.com) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] subscribe digest Message-ID: <1ca.f8f4d9e.2c760f56@aol.com> subscribe digest -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/9a84ad45/attachment.htm From louise_renton <@t> hotmail.com Thu Aug 21 08:02:58 2003 From: louise_renton <@t> hotmail.com (louise renton) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Calcium stain Message-ID: Dear All, Is it possible/feasible to do either a Von Kossa or an Alizarin red stain on PMMA sections. If so, which is the more sensitive? I am, of course, open to suggestions of other stains excluding IHC. Best regards Louise Renton Bone Research Unit MRC Johannesburg South Africa Tel & fax +27 11 717 2298 "Time flies like an arrow, fruit flies like a banana" _________________________________________________________________ Rain coat or t-shirt? Find out with MSN Weather http://www.msn.co.za/weather/ From pmarcum <@t> polysciences.com Thu Aug 21 07:53:52 2003 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:21:46 2005 Subject: [Histonet] Virus Message-ID: <002001c367e3$45d68670$4800a8c0@PMARCUM2K> Good Morning, I'm not sure who is infected at this point however, I just got 46 e-mails and all of the mail from Histonet was related to "the movie, details or some other subject line" that was part of the worm thing. I am afraid to open anything from HistoNet at this point unless the title is related to a subject having to do with Histology directly. I am so sorry you are all trying so hard to get this fixed with the new server and what we really need right now is to know where these are coming from and how to stop it. We have run virus checks here and we are not the source. Should we unsubscribe and then re-subscribe to see if that helps? Pam Marcum > -----Original Message----- > From: Pamela Marcum [mailto:pmarcum@polysciences.com] > Sent: Wednesday, August 20, 2003 8:44 AM > To: Macke, Gail; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Virus > > > I had a whole collection of them this morning on my incoming > mail. I'm not sure where it started but several topics were in > the subject line and included mail from several companies, and a > number of individuals all through HistoNet. At lest my > opportunities to save third world dictators is only coming from > other sources. Some days I have up to fifteen of those along > with my regular mail. I love HistoNet and the EM server except > on these kinds of days. Everyone at HistoNet and the EM Server > tries so very hard to avoid these things and it just impossible > to do with some of these unauthorized people who only use a name > to get on line and send mail. Individuals are much harder to > deal with in e-mail. Pam Marcum > > > -----Original Message----- > > From: histonet-admin@lists.utsouthwestern.edu > > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Macke, Gail > > Sent: Wednesday, August 20, 2003 8:29 AM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] Virus > > > > > > Dear all, > > Our fire wall has remove three different email from Histonet with > > viruses in them. Said something about the Thank you virus has > been removed > > from this email. One is from Hackerlabs and two from a Mary both at > > AOL.com. Just thought I'd let everyone know to fore warn you if your > > firewall doesn't catch them. All three messages had the "(Histonet)" on > > them. > > Gail Macke,HTL > > Shriners Burns Hospital > > CONFIDENTIALITY NOTICE: This e-mail communication and any > attachments may > > contain confidential and privileged information for the use of the > > designated recipients. If you are not the intended recipient, (or > > authorized to receive for the recipient) you are hereby > notified that you > > have received this communication in error and that any review, > disclosure, > > dissemination, distribution or copying of it or its contents is > > prohibited. > > If you have received this communication in error, please destroy > > all copies > > of this communication and any attachments and contact the > sender by reply > > e-mail or telephone (813) 281-0300. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From pmarcum <@t> polysciences.com Thu Aug 21 08:50:30 2003 From: pmarcum <@t> polysciences.com (Pamela Marcum) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Virus In-Reply-To: <002001c367e3$45d68670$4800a8c0@PMARCUM2K> Message-ID: <002f01c367eb$2f284690$4800a8c0@PMARCUM2K> Dear all on Histonet, I received an e-mail from Gayle Macke and I owe her and anyone who thought I was saying she was to blame this explanation. I was afraid to open yet another avenue for this by sending a new e-mail through HistoNet. I in no way thought you were blaming me or anyone else on HistoNet. We were all hit the same way. Please accept my apology if it made you feel you were to blame. I am getting e-mails from everyone on the this and several other professional list who got hit. Pam Marcum > -----Original Message----- > From: histonet-admin@lists.utsouthwestern.edu > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Pamela > Marcum > Sent: Thursday, August 21, 2003 8:54 AM > To: Pamela Marcum; Macke, Gail; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Virus > > > Good Morning, > > I'm not sure who is infected at this point however, I just got 46 e-mails > and all of the mail from Histonet was related to "the movie, > details or some > other subject line" that was part of the worm thing. I am afraid to open > anything from HistoNet at this point unless the title is related to a > subject having to do with Histology directly. > > I am so sorry you are all trying so hard to get this fixed with the new > server and what we really need right now is to know where these are coming > from and how to stop it. We have run virus checks here and we are not the > source. Should we unsubscribe and then re-subscribe to see if that helps? > > Pam Marcum > > > -----Original Message----- > > From: Pamela Marcum [mailto:pmarcum@polysciences.com] > > Sent: Wednesday, August 20, 2003 8:44 AM > > To: Macke, Gail; 'histonet@lists.utsouthwestern.edu' > > Subject: RE: [Histonet] Virus > > > > > > I had a whole collection of them this morning on my incoming > > mail. I'm not sure where it started but several topics were in > > the subject line and included mail from several companies, and a > > number of individuals all through HistoNet. At lest my > > opportunities to save third world dictators is only coming from > > other sources. Some days I have up to fifteen of those along > > with my regular mail. I love HistoNet and the EM server except > > on these kinds of days. Everyone at HistoNet and the EM Server > > tries so very hard to avoid these things and it just impossible > > to do with some of these unauthorized people who only use a name > > to get on line and send mail. Individuals are much harder to > > deal with in e-mail. Pam Marcum > > > > > -----Original Message----- > > > From: histonet-admin@lists.utsouthwestern.edu > > > [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of > Macke, Gail > > > Sent: Wednesday, August 20, 2003 8:29 AM > > > To: 'histonet@lists.utsouthwestern.edu' > > > Subject: [Histonet] Virus > > > > > > > > > Dear all, > > > Our fire wall has remove three different email from Histonet with > > > viruses in them. Said something about the Thank you virus has > > been removed > > > from this email. One is from Hackerlabs and two from a Mary both at > > > AOL.com. Just thought I'd let everyone know to fore warn you if your > > > firewall doesn't catch them. All three messages had the > "(Histonet)" on > > > them. > > > Gail Macke,HTL > > > Shriners Burns Hospital > > > CONFIDENTIALITY NOTICE: This e-mail communication and any > > attachments may > > > contain confidential and privileged information for the use of the > > > designated recipients. If you are not the intended recipient, (or > > > authorized to receive for the recipient) you are hereby > > notified that you > > > have received this communication in error and that any review, > > disclosure, > > > dissemination, distribution or copying of it or its contents is > > > prohibited. > > > If you have received this communication in error, please destroy > > > all copies > > > of this communication and any attachments and contact the > > sender by reply > > > e-mail or telephone (813) 281-0300. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Ngilman <@t> nbhd.org Thu Aug 21 09:11:00 2003 From: Ngilman <@t> nbhd.org (Noreen Gilman) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] unsubscribe Message-ID: Noreen Gilman, BS, HT (ASCP) QIHC Histopathology Supervisor Broward General Medical Center Ft. Lauderdale, FL 33316 954.355.4358 Phone 954.355.4139 Fax 954.387.0213 Pager -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/ae79edea/attachment.htm From fcs <@t> uevora.pt Thu Aug 21 09:28:14 2003 From: fcs <@t> uevora.pt (Fernando Capela e Silva) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] unsubscribe References: Message-ID: <002701c367f0$755dcf40$d6d988c1@uevora.pt> [Histonet] unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/ea8bc9c2/attachment.htm From mandy_annis <@t> usgs.gov Thu Aug 21 09:43:47 2003 From: mandy_annis <@t> usgs.gov (Mandy Annis) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Shandon Excelsior Tissue Processor Message-ID: We are interested in purchasing a Shandon Thermo -Shandon Excelsior Tissue Processor. Upon searching the archives I could not find any comments on this machine. Does anyone have any experiences that they could share regarding this machine? Thanks in advance! Mandy ********************************************************************** Mandy L. Annis Columbia Environmental Research Center 4200 New Haven RD Columbia, MO 65201 Phone:(573)-441-2940 Fax:(573)-876-1896 e-mail:mandy_annis@usgs.gov From cwscouten <@t> myneurolab.com Thu Aug 21 10:21:46 2003 From: cwscouten <@t> myneurolab.com (Charles W. Scouten, Ph.D.) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] cryostat decontamination Message-ID: With the Vibratome 7500 Cryostat, decontamination involves pressing a button and doing something else for 3.5 hours, after which the instrument is thoroughly decontaminated, again cold, and ready to cut tissue. Before the next decontamination cycle, empty the waste bin down the sink, and refill a bottle of water and a bottle of Perascope decontamination fluid. That's all. For more information on how this is accomplished, see the following link: http://www.myneurolab.com/mnl/mnlsite/ViewProduct.asp?idproduct=475102&catdesc=Histology+Equipment&subcatdesc=Cryostats&idsubcategory=182 Charles W.? Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300? FAX? 314 522 0377 cwscouten@myneurolab.com www.myneurolab.com -----Original Message----- From: Bill Sinai [mailto:bills@icpmr.wsahs.nsw.gov.au] Sent: Wednesday, August 20, 2003 4:57 PM To: Laurie Colbert; histonet Subject: Re: [Histonet] cryostat decontamination Laura, We have a protocol for decontaminating all our Cryostats once per week. We have 2 Shandon 660E models which have the heating block which you fill with 40% formadlehyde then turn on the decontamination cycle. This is performed overnight Friday. After this process the shavings are removed using tissue damped with 70% ethanol. The others have a formaldehyde "bomb" prepared and placed into the chamber, then left sealed to defrost for 24 hours. These are then cleaned using 70% ethanol. The same procedures are carried out if we have cut anything suspected of being an infective agent, immediately after the case is finished. We have the luxury of two cryostats available in our main laboratory. All the best Bill Sinai Laboratory Manager Tissue Pathology ICPMR P.O. Box 533 Wentworthville NSW 2145 pH 02 9845 7774 ----- Original Message ----- From: "Laurie Colbert" To: Sent: Thursday, August 21, 2003 5:03 AM Subject: [Histonet] cryostat decontamination How do others clean the cryostat after a known or suspected TB case? Also, do you decontaminate once a week with a tuberculocidal antiseptic? Laurie Colbert Huntington Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgibbon <@t> qltinc.com Thu Aug 21 10:38:18 2003 From: kgibbon <@t> qltinc.com (kgibbon@qltinc.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] storing digital images Message-ID: Hi All, This is a question aimed at those who store digital images in their patient records. Is their a standard set by the ASCP or any other institution that gives recommendations on the format for storing digital images in patient records?? Do the images have to survive in the same way that the patient record has to be kept? I am looking to a standard that can be used to archive images for a long period of time so any advice would be greatly appreciated thanks Kevin Gibbon QLT Inc. This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. From ander093 <@t> gold.tc.umn.edu Thu Aug 21 10:54:12 2003 From: ander093 <@t> gold.tc.umn.edu (LuAnn Anderson) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Background on virus Message-ID: <5.2.0.9.0.20030821105231.009fa100@ander093.email.umn.edu> From CNN news: SoBig Virus Is So Worrying for Computer Users By Bernhard Warner LONDON (Reuters) - A new computer virus feared to be the most potent ever spread like wildfire Thursday, sending e-mail networks crashing and frazzling technicians already overstretched by a plague of computer bugs. The SoBig virus spreads when unsuspecting computer users open file attachments in e-mails that contain such familiar headings as "Thank you," "Re: Details" or "Re: approved." Once the file is opened, SoBig, which first appeared Monday, scours the computer for e-mail addresses, checking in Word documents, Internet logs and e-mail inboxes. Designed like mass-mailing spam programs, it then sends scores of messages to the addresses it has collected. MessageLabs, a British-based Internet security firm, said one in 17 e-mails sent around the world since Monday had been affected by SoBig. MessageLabs' chief information analyst Paul Wood said it was feared that it could increase global e-mail traffic by as much as 60 percent, slowing the Internet to a crawl. "It's unprecedented in our history. We stopped over one million (infections) in the first day," he said. "It's a pretty frightening statistic. And the next incarnation could be even worse." Technicians have been scrambling for the past week to fend off the most concentrated digital onslaught ever seen. From bjhighto <@t> utmb.edu Thu Aug 21 11:05:49 2003 From: bjhighto <@t> utmb.edu (Hightower, Barbara J.) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] unsubcribe Message-ID: <2456B984836CD611B61500D0B79E87E003518410@exchange2> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/f6416fab/attachment.htm From lizchlipala <@t> premierhistology.com Thu Aug 21 11:19:39 2003 From: lizchlipala <@t> premierhistology.com (Elizabeth Chlipala) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] PASM problem Message-ID: <000d01c36800$087eeb10$74d48a80@LIZ> Hello All We have been working on the Jones stain for kidney basement membranes. We have our internal control specimen which is a human specimen. The other specimens we are staining are rat kidneys. When we stain the slides, the human kidney comes out great, very good staining of the basement membranes, very slight nuclear staining with no precipitate. The rat kidneys don't look as nice, there is good staining of the basement membrane, but the nuclear staining is more predominant and there is intense granular staining in the cytoplasm, which sometimes looks like a precipitate. Does anyone have any suggestions? Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP) Premier Histology Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 lizchlipala@premierhistology.com www.premierhistology.com Ship to Address: Premier Histology Laboratory University of Colorado MCBD, Room A3B40 Boulder, Colorado 80309 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/1ea03a0e/attachment.htm From tpmorken <@t> labvision.com Thu Aug 21 11:43:17 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] storing digital images Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C411117C@usca0082k03.rallansci.apogent.com> Kevin, There is a standard for medical imaging, but it has not been adopted by any anatomic pathology LIS vendors as yet. It is used in radiology right now. So the answer for you now is, no there is no standard for pathology. Here is the standards homepage: http://medical.nema.org/ Here is more info from a coming conference http://apiii.upmc.edu/programs/focus.html#digital API Working Group Session - Digital Imaging Standards in Pathology Wednesday, October 8, 2003 11:30 a.m. - 1 p.m. Marriott City Center, Conference Room B Limited to 24 participants It is now the 20th anniversary of DICOM (Digital Image Communications in Medicine), which has become the lingua franca for Teleradiology. This standard has supported an explosive growth in the utilization of digital imaging and electronic information interchange technologies in routine clinical practice of diagnostic radiology. While an extension to the DICOM standard for pathology has been in place since 1998, no APLIS vendor to date has implemented the Visible Light supplement for production-oriented exchange of diagnostic pathology images. This session provides salient background as to why such an implementation of a DICOM-compliant imaging system / server in your practice may be desirable. This session begins with an introduction to the DICOM standard with emphasis on the Visible Light Image Object Definition (IOD) and how it can be used in the setting of pathology. The steps needed to take a standard jpeg image and annotate it to become a valid DICOM object will be reviewed. Also, current developments in the DICOM Visible Light Working Group will be presented as a segue into a general discussion, which will focus upon ways of encouraging pathologists and especially APLIS vendors to implement and support DICOM-compatible imaging solutions. The session is intended to result in the formation of a Working Group, which will encourage the use of DICOM within pathology by authoring a technical report and possibly developing a toolkit for creating DICOM objects from pathologic images. Tim Morken Product Development Lab Vision / NeoMarkers 47790 Westinghouse Dr Fremont, CA 94539 PH: 510-991-2840 www.labvision.com -----Original Message----- From: kgibbon@qltinc.com [mailto:kgibbon@qltinc.com] Sent: Thursday, August 21, 2003 8:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] storing digital images Hi All, This is a question aimed at those who store digital images in their patient records. Is their a standard set by the ASCP or any other institution that gives recommendations on the format for storing digital images in patient records?? Do the images have to survive in the same way that the patient record has to be kept? I am looking to a standard that can be used to archive images for a long period of time so any advice would be greatly appreciated thanks Kevin Gibbon QLT Inc. This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From healthqs <@t> yahoo.co.uk Thu Aug 21 11:46:44 2003 From: healthqs <@t> yahoo.co.uk (=?iso-8859-1?q?cha=20kai-whitewind?=) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Histonet] unsubcribe In-Reply-To: <2456B984836CD611B61500D0B79E87E003518410@exchange2> Message-ID: <20030821164644.6052.qmail@web14701.mail.yahoo.com> --------------------------------- Want to chat instantly with your online friends??Get the FREE Yahoo!Messenger -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030821/ff0d6b28/attachment.htm From CCLYATT <@t> mail.mcg.edu Thu Aug 21 14:42:13 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Job Descriptions/Evaluations Message-ID: Histonetters, My department is looking at reorganizing our job titles. We are looking to provide a career ladder for technicians. If you can share your job descriptions, evaluation criteria and title structure, I would be very appreciative. Thanks, Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu From AnthonyH <@t> chw.edu.au Thu Aug 21 20:03:49 2003 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] storing digital images Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E00E@simba.kids> We archive our images to CD-Rs. We batch the cases (eg 4 months worth)and burn 2 copies. These CD's will then be copied in 4 years time in case of deterioration of the originals. After burning, a sample of the images are viwed to ensure data integrity. This seems the cheapest method of ensuring data integrity and easy access. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html -----Original Message----- From: kgibbon@qltinc.com [mailto:kgibbon@qltinc.com] Sent: Friday, 22 August 2003 1:38 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] storing digital images Hi All, This is a question aimed at those who store digital images in their patient records. Is their a standard set by the ASCP or any other institution that gives recommendations on the format for storing digital images in patient records?? Do the images have to survive in the same way that the patient record has to be kept? I am looking to a standard that can be used to archive images for a long period of time so any advice would be greatly appreciated thanks Kevin Gibbon QLT Inc. This electronic transmission (including any and all attachments) is intended solely for the use of the individual or entity to whom it is addressed and may contain information that is privileged and/or confidential. If you are not the intended recipient of this electronic transmission, you are hereby notified that any disclosure, copying or distribution, or the taking of any action in reliance upon the contents of this electronic transmission, is strictly prohibited, and you are further requested to purge this electronic transmission and all copies thereof from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mward <@t> wfubmc.edu Fri Aug 22 07:58:23 2003 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] frozen tissue Message-ID: <61135F0455D33347B5AAE209B903A304F7BDDF@EXCHVS2.medctr.ad.wfubmc.edu> I have a situation that I hope I can get some help with. My neuropahtologist had some nerve tissue in formalin for several weeks. He gave the tissue to a resident, had him bisect the tissue and asked him to order immunofluorescent studies (IgG, IgM). He told the resident to wash the tissue in water and OCT embedding media, freeze and cut sections. Has anyone ever tried this and if so how did things turn out? Thanks in advance for the help. Martha Ward Wake Forest University Baptist Medical Center From janis-rodgers <@t> uiowa.edu Fri Aug 22 08:53:09 2003 From: janis-rodgers <@t> uiowa.edu (Rodgers, Janis) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] (no subject) Message-ID: <5D03ED7B9391D4119D9B0008C76B7B2402D45EF3@uihc-mail1.uihc.uiowa.edu> unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030822/50b98630/attachment.htm From juan.gutierrez <@t> christushealth.org Fri Aug 22 09:19:59 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] frozen tissue Message-ID: Yes, and it doesn't work. That's why we use transport media. The only time we got it to work was when the tissue had been in formalin for only a few minutes. Good luck! Juan -----Original Message----- From: Martha Ward [mailto:mward@wfubmc.edu] Sent: Fri 8/22/2003 7:58 AM To: histonet@pathology.swmed.edu Cc: Subject: [Histonet] frozen tissue I have a situation that I hope I can get some help with. My neuropahtologist had some nerve tissue in formalin for several weeks. He gave the tissue to a resident, had him bisect the tissue and asked him to order immunofluorescent studies (IgG, IgM). He told the resident to wash the tissue in water and OCT embedding media, freeze and cut sections. Has anyone ever tried this and if so how did things turn out? Thanks in advance for the help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Fri Aug 22 09:17:32 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] manual immunos Message-ID: Have you looked at the sequenza system by thermo-electron(formerly shandon). I have had good luck with this in the past. www.thermo.com/shandon Fred -----Original Message----- From: "Tague, Curtis" Sent: Tuesday, August 19, 2003 5:21 PM To: "Histonet (E-mail)" Subject: [Histonet] manual immunos I read something a while ago about a manual immunostainer or incubator made by a company out of Logan Utah (I think) but can't find the message or the company on a search. It was a slick little machine and I would like to take a look at it again, just for fun. If you are familiar with this product or company, please reply. Thanks. Confidentiality Note: The preceding e mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Fri Aug 22 10:23:01 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] frozen tissue Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C4111187@usca0082k03.rallansci.apogent.com> The problems with using formalin-fixed tissue for frozen/fluorescent studies is that 1) formalin-fixed tissue has high autofluoresence, and 2) formalin-fixed frozen tissue does not stick to slides very well. It may be that you can get acceptiable IgG and IgM staining with normal IHC procedures. I've done that on kidney tissue in which the frozen material was inadequate. The formalin tissue showed good staining and was acceptable for diagnosis. Tim Morken Lab Vision / NeoMarkers -----Original Message----- From: Martha Ward [mailto:mward@wfubmc.edu] Sent: Friday, August 22, 2003 5:58 AM To: histonet@pathology.swmed.edu Subject: [Histonet] frozen tissue I have a situation that I hope I can get some help with. My neuropahtologist had some nerve tissue in formalin for several weeks. He gave the tissue to a resident, had him bisect the tissue and asked him to order immunofluorescent studies (IgG, IgM). He told the resident to wash the tissue in water and OCT embedding media, freeze and cut sections. Has anyone ever tried this and if so how did things turn out? Thanks in advance for the help. Martha Ward Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Fri Aug 22 10:46:24 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Job Descriptions/Evaluations Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C4111188@usca0082k03.rallansci.apogent.com> Claye, that is an interesting subject; If you get a lot of private replies it would be interesting for the rest of us to see how other labs handle that. >From my experience, the more levels of responsibility you have the easier it is to have a "career ladder." Many labs have just bench tech, and supervisor (or "team leader" these days). If you can breakout all the various levels of technical expertise you can build in several levels. It is easier in a large/complex lab than a small/non-complex lab however. I don't think people should "move up" just because they occupy a chair for a given number of years. They have to take on more responsibility either in procedures or supervision. Even passing a certification does not necessarily mean they deserve a different title - they still have to take on the responsibility. Some basic ideas Entry level- routine sectioning, routine stains and easy special stains, gross assist, general grunt work. Bench tech - add more difficult cutting/higher production level cutting, special stains, frozen sections. Senior tech - add specialized cutting, advanced specials, simple grossing, assist with procedure development and writing, Teach procedures, methodology, theory to others Special procedures tech - add muscle enzyme stains, immunochemistry, more advanced grossing, assist with procedure development and writing, Teach procedures, methodology, theory to others Asst supervisor - add supervise several other techs at any level in an area (ie sectioning, specials, immunos), assist with inventory, budgeting, cost-account, oversee procedure development and writing, Teach procedures, methodology, theory to others Supervisor - Ride herd and take grief for everything Tim Morken -----Original Message----- From: Claye Clyatt [mailto:CCLYATT@mail.mcg.edu] Sent: Thursday, August 21, 2003 12:42 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Job Descriptions/Evaluations Histonetters, My department is looking at reorganizing our job titles. We are looking to provide a career ladder for technicians. If you can share your job descriptions, evaluation criteria and title structure, I would be very appreciative. Thanks, Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CWilsonCPI <@t> ameripath.com Fri Aug 22 15:23:43 2003 From: CWilsonCPI <@t> ameripath.com (CWilsonCPI@ameripath.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] reprocessing tissue on a slide? Message-ID: has anyone ever heard of taking a slide back to formalin, and then reprocessing????? Help, this is all the tissue I have left on a small GI biopsy that was poorly processed.... Thanks, Carol Wilson Lab Supervisor AmeriPath Cleveland CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message From mjane <@t> uab.edu Fri Aug 22 15:38:22 2003 From: mjane <@t> uab.edu (Jane Hosmer) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Histonet(Unsubscribe) Message-ID: <5.2.1.1.0.20030822153705.00ac2e10@norm.dpo.uab.edu> Please take me off!!!!!!! From hho <@t> neurology.bsd.uchicago.edu Fri Aug 22 15:50:23 2003 From: hho <@t> neurology.bsd.uchicago.edu (hho@neurology.bsd.uchicago.edu) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Tissue Processing Protocol for Paraffin sectioning Message-ID: Dear Histonetters, Does anybody have a good set of protocol for tissue processing I can try it out? eg. Mice Brain, spinal cord.. muscle. If do please reply or e-mail me please! =) Hanson Ho From LMARGRAF <@t> childmed.dallas.tx.us Fri Aug 22 16:03:09 2003 From: LMARGRAF <@t> childmed.dallas.tx.us (LINDA MARGRAF MD) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] IMPORTANT HISTONET INFORMATION Message-ID: Dear Histonetters: Here is some more information about the new server we are using for Histonet. To access information about your subscription or to change something go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet and follow the instructions.(You will need to scroll down to the Histonet subscriber area). Once you put in your email address in the correct field it will take you to a page that shows what functions are selected for your address and you can change to digest mode etc. You will need your password to make changes. You received your password in the email Herb sent Saturday but if you lost it you can request it again from the server (also at this address). The server will email the password to you again. SPECIAL NOTE TO DIGEST SUBSCRIBERS: Herb spent many hours switching people who used to be on the digest back to the digest yesterday so you won't need to make that change yourself now. The number of messages with "subscribe digest" in the subject line indicated to us people are still trying to learn the new system. NOTE TO PEOPLE WHO WANT TO GET OFF THE LIST: You will need to go to the above email address and unsubscribe. You will need your password to get off the list. If you have problems or your address has changed enough since you first subscribed that the server doesn't recognize you let me know. TO SEND MESSAGES TO CIRCULATE TO THE LIST: Send the email messages you wish to send to the subscribers to: histonet@lists.utsouthwestern.edu Please do not send subscribe, unsubscribe requests to this address NOTE ABOUT VIRUSES: Several nasty viruses are now circulating around the internet and clearly some subscribers have some. The server is designed to clear out viruses from the email before it circulates to the list so it is unlikely you will get a virus from a Histonet message. However, always be cautious about attachments and mail from sources you don't recognize. Thanks for being patient as we all get used to the new server. In the long run it will be a better system. Linda M Histonet administrator From jschuma1 <@t> FAIRVIEW.ORG Fri Aug 22 11:01:44 2003 From: jschuma1 <@t> FAIRVIEW.ORG (JENNIFER SCHUMACHER) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Job Descriptions/Evaluations Message-ID: Please post responses to the list serve. We are also talking about doing this, especially to develop a difference for HT vs. HTL. Thanks. Jennifer >>> "Claye Clyatt" 08/21/03 02:42PM >>> Histonetters, My department is looking at reorganizing our job titles. We are looking to provide a career ladder for technicians. If you can share your job descriptions, evaluation criteria and title structure, I would be very appreciative. Thanks, Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030822/ec58d3b1/attachment.htm From Milton.Gomez <@t> msnyuhealth.org Fri Aug 22 16:53:02 2003 From: Milton.Gomez <@t> msnyuhealth.org (Gomez, Milton) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] IMPORTANT HISTONET INFORMATION Message-ID: <56187BC57751AA4EB5E81528D2D4972A0E40A2@excebw2k17.msnyuhealth.org> Dear Dr. Margraf: Are any of these Histonet replies being archived somewhere? For example, if I want to check a particular question and answer that may be useful to me, would I be able to find it? Thank you, Milton A. Gomez -----Original Message----- From: LINDA MARGRAF MD [mailto:LMARGRAF@childmed.dallas.tx.us] Sent: Friday, August 22, 2003 5:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IMPORTANT HISTONET INFORMATION Dear Histonetters: Here is some more information about the new server we are using for Histonet. To access information about your subscription or to change something go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet and follow the instructions.(You will need to scroll down to the Histonet subscriber area). Once you put in your email address in the correct field it will take you to a page that shows what functions are selected for your address and you can change to digest mode etc. You will need your password to make changes. You received your password in the email Herb sent Saturday but if you lost it you can request it again from the server (also at this address). The server will email the password to you again. SPECIAL NOTE TO DIGEST SUBSCRIBERS: Herb spent many hours switching people who used to be on the digest back to the digest yesterday so you won't need to make that change yourself now. The number of messages with "subscribe digest" in the subject line indicated to us people are still trying to learn the new system. NOTE TO PEOPLE WHO WANT TO GET OFF THE LIST: You will need to go to the above email address and unsubscribe. You will need your password to get off the list. If you have problems or your address has changed enough since you first subscribed that the server doesn't recognize you let me know. TO SEND MESSAGES TO CIRCULATE TO THE LIST: Send the email messages you wish to send to the subscribers to: histonet@lists.utsouthwestern.edu Please do not send subscribe, unsubscribe requests to this address NOTE ABOUT VIRUSES: Several nasty viruses are now circulating around the internet and clearly some subscribers have some. The server is designed to clear out viruses from the email before it circulates to the list so it is unlikely you will get a virus from a Histonet message. However, always be cautious about attachments and mail from sources you don't recognize. Thanks for being patient as we all get used to the new server. In the long run it will be a better system. Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfarm <@t> sio.midco.net Fri Aug 22 17:28:58 2003 From: cfarm <@t> sio.midco.net (celia) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] warthin starry stain Message-ID: <000001c36976$8a5bdf40$0100a8c0@youru10ixi0anw> I am having some trouble with our Warthin-Starry stain. The peripheri of the gastric biopsy is not getting stained. The rest of the specimen is well stained. I am not sure if I need to lengthen the impregnation time or the developer time. I have never had this happen before. Any suggestions would be greatly appreciated. Celia, HT Sioux Valley Hospital -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030822/a86a2b2e/attachment.htm From lpwenk <@t> mail.netquest.com Sat Aug 23 08:44:13 2003 From: lpwenk <@t> mail.netquest.com (Lee & Peggy Wenk) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] warthin starry stain References: <000001c36976$8a5bdf40$0100a8c0@youru10ixi0anw> Message-ID: <005601c3697c$a3e71f00$8732fea9@hppav> It's probably because the doctor/nurse is letting the biopsy dry out before they place it in formalin. Even setting a minute on a pad will do this. We've seen this also with kidney biopsies. No way to correct it after the fact. No way to make the W/S stain work. The tissue HAS to be placed in the fixative IMMEDIATELY. You might try the Giemsa or Diff-Quik stain. Sometimes they will still stain the H. pylorii after this drying out. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ----- Original Message ----- From: celia To: histonet@pathology.swmed.edu Sent: Friday, August 22, 2003 6:28 PM Subject: [Histonet] warthin starry stain I am having some trouble with our Warthin-Starry stain. The peripheri of the gastric biopsy is not getting stained. The rest of the specimen is well stained. I am not sure if I need to lengthen the impregnation time or the developer time. I have never had this happen before. Any suggestions would be greatly appreciated. Celia, HT Sioux Valley Hospital -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030823/3b326923/attachment.htm From vbaker60 <@t> yahoo.com Sat Aug 23 08:52:37 2003 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] warthin starry stain In-Reply-To: <000001c36976$8a5bdf40$0100a8c0@youru10ixi0anw> Message-ID: <20030823135237.17822.qmail@web12108.mail.yahoo.com> Celia, How does your positive control look? Have you changed anything in your protocol for processing or staining? Have you checked the pH of your DI water before acidulation and then after? Warthin Starry is ultra sensitive, one small change can alter your results. It could be anything from a change in temperature in processing to not having the tissue sufficiently cleared or hydrated. If you have spirochetes in your positive control and not a lot of precipitate meaning it's clean) it could be that your processing may have something to do with it. If the processor gets too hot during the dehydration phase it sort of "burns" the tissue. Also are you using control material from an outside source or one that has been processed in your lab? Warthin Starry is among the more sensitive silver stains and it has well earned the second name it was given "Worthless & Sorry" for a very good reason. As a last resort, do a Giemsa it will give you the same results with half the grief. Vikki Baker Institute for Cancer Prevention Valhalla, New York __________________________________ Do you Yahoo!? Yahoo! SiteBuilder - Free, easy-to-use web site design software http://sitebuilder.yahoo.com From smcassar <@t> waldonet.net.mt Sun Aug 24 07:38:00 2003 From: smcassar <@t> waldonet.net.mt (Sharon Cassar) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] (no subject) Message-ID: <05ce01c36a3c$8ee41480$3b65ccc2@default> Unsubscribe -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030824/15a75d28/attachment.htm From badesuyi <@t> hotmail.com Sun Aug 24 09:39:11 2003 From: badesuyi <@t> hotmail.com (Banjo Adesuyi) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] HTL(ASCP) PRACTICAL TEST Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030824/c5f4c461/attachment.htm From badesuyi <@t> hotmail.com Sun Aug 24 09:39:25 2003 From: badesuyi <@t> hotmail.com (Banjo Adesuyi) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] HTL(ASCP) PRACTICAL TEST Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030824/9e668735/attachment.htm From rmaddox <@t> umich.edu Sun Aug 24 09:53:54 2003 From: rmaddox <@t> umich.edu (Rachel D Maddox) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Saff O/ Fast Green In-Reply-To: Message-ID: Hi. I'm looking for some advice/ information on using saffranin O and fast green for staining sections that contain both articular cartilage and bone tissue. The saffranin O stains for glycosamino glycans (GAGs), which are present in the cartilage, while the fast green stains for collagen, making the bone appear green-brown-grey. All the information I can find simply indicates that fast green stains for collagen, but does not indicate whether or not is specific for collagen I. I am wondering whether there is such specificity and if not, what prevents the fast green from staining the abundant type II collagen in the cartilage? Any information would be of great help. Thanks, From JGordon <@t> cellmarque.com Sun Aug 24 18:14:53 2003 From: JGordon <@t> cellmarque.com (JGordon@cellmarque.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Re: Your application Message-ID: A non-text attachment was scrubbed... Name: not available Type: multipart/mixed Size: 214 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030824/6116c899/attachment.bin From anissachoi <@t> hotmail.com Sun Aug 24 20:18:24 2003 From: anissachoi <@t> hotmail.com (Anissa Choi) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Re: Haematoxylin in Ventana Immuno-stainer Message-ID: I am experimenting to use home made haematoxylin in the Ventana stainer . I have tried Mayer's Haematoxylin and Carazzi's Haematoxylin. The results are unsatisfactory as there are precipitates on both the slides and the slides tray inside the stainer at the end of the run. I think the temperature inside the machine may have caused this problem. I wonder is there something I can add to the Hx to make it work. Any inputs will be greatly appreciated. Thanks in advance. Anissa Choi Burnaby Hospital Canada _________________________________________________________________ Help STOP SPAM with the new MSN 8 and get 2 months FREE* http://join.msn.com/?page=features/junkmail From c.m.vanderloos <@t> amc.uva.nl Mon Aug 25 01:40:26 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] RE: frozen tissue Message-ID: <8bb6cf8bf001.8bf0018bb6cf@amc.uva.nl> Hi Martha, How about embedding the tissue in paraffin as usual and perform Immunogold-silver staining? The accuracy is as good as fluorescence and you also have the option of "epipolarization microscopy" using an epipolarization (or IGS block) in your fluorescence scope. The result certainly competes with immunofluorescence. Please have a look at www.aurion.nl for info about this rather unknown but very elegant IHC technique. Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands >From: Martha Ward [mailto:mward@wfubmc.edu] >Sent: Fri 8/22/2003 7:58 AM >To: histonet@pathology.swmed.edu >Cc: >Subject: [Histonet] frozen tissue > >I have a situation that I hope I can get some help with. My >neuropahtologist had some nerve tissue in formalin for several weeks. >He gave the tissue to a resident, had him bisect the tissue and asked >him to order immunofluorescent studies (IgG, IgM). He told the >resident to wash the tissue in water and OCT embedding media, freeze >and cut sections. Has anyone ever tried this and if so how did things >turn out? Thanks in advance for the help. > >Martha Ward >Wake Forest University Baptist Medical Center From c.m.vanderloos <@t> amc.uva.nl Mon Aug 25 01:48:26 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] RE: frozen tissue Message-ID: <8c20c28bd98d.8bd98d8c20c2@amc.uva.nl> Hi Martha, How about embedding the tissue in paraffin as usual and perform Immunogold-silver staining? The accuracy is as good as fluorescence and you also have the option of "epipolarization microscopy" using an epipolarization (or IGS block) in your fluorescence scope. The result certainly competes with immunofluorescence. Please have a look at www.aurion.nl for info about this rather unknown but very elegant IHC technique. Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center Amsterdam - The Netherlands >From: Martha Ward [mailto:mward@wfubmc.edu] >Sent: Fri 8/22/2003 7:58 AM >To: histonet@pathology.swmed.edu >Cc: >Subject: [Histonet] frozen tissue > >I have a situation that I hope I can get some help with. My >neuropahtologist had some nerve tissue in formalin for several weeks. >He gave the tissue to a resident, had him bisect the tissue and asked >him to order immunofluorescent studies (IgG, IgM). He told the >resident to wash the tissue in water and OCT embedding media, freeze >and cut sections. Has anyone ever tried this and if so how did things >turn out? Thanks in advance for the help. > >Martha Ward >Wake Forest University Baptist Medical Center From mghoddusi <@t> cmri.usyd.edu.au Mon Aug 25 02:21:26 2003 From: mghoddusi <@t> cmri.usyd.edu.au (Majid Ghoddusi) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] IHC- using IgM vs. IgG Message-ID: Hello dear histonetters Just a very basic question for IHC experts: if your primary antibody is a mouse IgM, by using a secondary anti-mouse IgG are you running any risk of getting artefactual signals? Thanks very much in advance for any help and advice including pointing to a reference that might discuss this in detail. ........................................................... Majid Ghoddusi Children's Medical Research Institute Locked Bag 23, Wentworthville NSW 2145 Tel: (02) 9687-2800 Fax:(02) 9687-2120 ............................................................. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030825/b79034d3/attachment.htm From Lizbeth_Kelly <@t> hgsi.com Mon Aug 25 05:48:07 2003 From: Lizbeth_Kelly <@t> hgsi.com (Lizbeth_Kelly@hgsi.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Histonet unsubscribe Message-ID: Please unsubscribe until further notice. Thank you, Lizbeth Kelly, HT (ASCP), QIHC Human Genome Sciences 240-314-4400 X2325 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030825/45dc2dbd/attachment.htm From hmcleod <@t> chempath.uct.ac.za Mon Aug 25 08:34:54 2003 From: hmcleod <@t> chempath.uct.ac.za (Mcleod) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] cyclin-D1 Message-ID: <3F4A107E.B7E1540E@chempath.uct.ac.za> Dear All What is the preferred AR method for Cyclin-D1's? We have the Dako (cat no M7155) clone DCS-6 antibody and while it works well as a marker for the cell cycle we have not had any success with Mantle Cell Lymphomas. We have tried the "standard" AR methods i.e. HIER at pH6 and pH.8 in a pressure cooker at full power for 2 mins. as well as protease on our Ventana Nexes system. Any help and suggestions very welcome. Thanks a stack in advance Heather. From cmconway <@t> usgs.gov Mon Aug 25 08:45:10 2003 From: cmconway <@t> usgs.gov (Carla M Conway) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] thymus differentiating help Message-ID: __________________ Hello, I have some cells in thymus sections that are positively stained by IHC, but I would like to identify them further. Could anyone suggest a stain that would differentiate between lymphocytes, macrophages and/or epithelial cells (stellated or elongated)? Thanks very much, Carla Conway Western Fisheries Research Center 6505 NE 65th St Seattle, WA 98115 ph: (206) 526-6282 ext. 242 fax: (206) 526-6654 From la.sebree <@t> hosp.wisc.edu Mon Aug 25 09:12:36 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] cyclin-D1 Message-ID: Hi Heather, Our protocol for Cyclin D1 (Neomarkers #MS-210-P, clone DCS-6) on our Ventana Benchmark is: CC1, P3/4", 1:50 for a 32" incubation. On our Ventana NexES our protocol is: 2" HIER in Biocare's decloaking chamber in Biocare's Nuclear Decloaking buffer, P3/8", 1:50 for a 24" incubation, Ventana amplification. Hope this helps; if you have any further questions, feel free to contact us. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Mcleod [mailto:hmcleod@chempath.uct.ac.za] Sent: Monday, August 25, 2003 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cyclin-D1 Dear All What is the preferred AR method for Cyclin-D1's? We have the Dako (cat no M7155) clone DCS-6 antibody and while it works well as a marker for the cell cycle we have not had any success with Mantle Cell Lymphomas. We have tried the "standard" AR methods i.e. HIER at pH6 and pH.8 in a pressure cooker at full power for 2 mins. as well as protease on our Ventana Nexes system. Any help and suggestions very welcome. Thanks a stack in advance Heather. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Mon Aug 25 10:37:49 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] IHC- using IgM vs. IgG Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C4111190@usca0082k03.rallansci.apogent.com> Majid asks: "if your primary antibody is a mouse IgM, by using a secondary anti-mouse IgG are you running any risk of getting artefactual signals?" Yes, your results will be negative. The anti-IgG will not detect the IgM primary antibody. Tim Morken Lab Vision / NeoMarkers Fremont, CA www.labvision.com -----Original Message----- From: Majid Ghoddusi [mailto:mghoddusi@cmri.usyd.edu.au] Sent: Monday, August 25, 2003 12:21 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHC- using IgM vs. IgG Hello dear histonetters Just a very basic question for IHC experts: if your primary antibody is a mouse IgM, by using a secondary anti-mouse IgG are you running any risk of getting artefactual signals? Thanks very much in advance for any help and advice including pointing to a reference that might discuss this in detail. ........................................................... Majid Ghoddusi Children's Medical Research Institute Locked Bag 23, Wentworthville NSW 2145 Tel: (02) 9687-2800 Fax:(02) 9687-2120 ............................................................. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030825/db715ae7/attachment.htm From SBarnes <@t> elch.org Mon Aug 25 11:39:08 2003 From: SBarnes <@t> elch.org (Sue Barnes) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Meditech users Message-ID: <3F74CB2B769A5843A0C746D3F13256FD1279E7@ELCH2> Procedures can be added to specimen types. We have mneumonics for each type of tissue we see. Attached to this is each procedure that is normally done. It defaults in and can be deleted if necessary. We also have views set up with the specimen type that will pull in results from the clinical and micro lab. This can be viewed by the pathologist while screening the slides. -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Monday, August 18, 2003 1:44 PM To: 'Tapper, Sheila'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Meditech users Sheila, I don't know what version you're working with but we have 4.9 and I may be wrong but I don't think you can link procedures to a specific tissue mnemonic. The only way that I know of is to create a new Medical Term for those specimens. At the Term Type prompt, enter TIS (tissue) then on the second screen, you can enter default procedures. You would then enter this as a procedure and it will explode the attached procedures. I have used it for bone marrows. I know it's not quite what you're looking for but entering one procedure is a little better than entering three. As for labels, we don't use them so I can't help you there. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: Tapper, Sheila [mailto:STapper@slhduluth.com] Sent: Monday, August 18, 2003 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech users Hi - We are currently implementing the Meditech computer system. I am interested in talking with anyone who has experience with building the Histology portion. We are experiencing great difficulty in trying to streamline the entry of specimens with tests attached to the specimens. For example: a liver biopsy with an iron, trichrome and retic stain attached. We would like to have the tests attached to the specimen, and be able to print labels for the slides as well. Can anyone out there help me find the answer? Thanks! Sheila Tapper St. Luke's Hospital Duluth, MN -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030825/5d3dbe25/attachment.htm From cfranci <@t> rigel.com Mon Aug 25 12:30:55 2003 From: cfranci <@t> rigel.com (Christian Franci) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Formalin blues Message-ID: I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris From nick.kirk3 <@t> btopenworld.com Mon Aug 25 13:19:11 2003 From: nick.kirk3 <@t> btopenworld.com (Nick Kirk) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Formalin blues In-Reply-To: Message-ID: Novocastra do one that works on formalin fixed paraffin processed tissue. Go to http://www.novocastra.co.uk/data/cam/Ig-super/cd311a10.pdf for further details Nick Kirk Head BMS Histopathology Hinchingbrooke Hospital Huntingdon England -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Christian Franci Sent: 25 August 2003 18:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin blues I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From romi <@t> stanford.edu Mon Aug 25 14:14:23 2003 From: romi <@t> stanford.edu (Romy Thomas) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Suggestions for the best anti-mouse Hedgehog Antibod(ies) Message-ID: Dear Wnt Pathway people, Can anybody refer me to a good company for hedgehog (Desert, Sonic, Indian) against mouse (primary & secondary OR fluorescent) for bone tissue purposes? Many thanks in advance Romy From wecare <@t> qualityhistology.com Mon Aug 25 14:52:11 2003 From: wecare <@t> qualityhistology.com (wecare@qualityhistology.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Inputs for new IHC Stainer design Message-ID: <003501c36b42$607379e0$0efea8c0@internetconnect.net> Dear Histonetters, We design and manufacture medical products. We are looking for your inputs in designing a new IHC stainer. Could you please let us know what you like the most about your IHC stainer? If you have a wish list of features that we should also incorporate in the new design, please include the list in your response. We thank you for your assistance in advance. Preyas Shah www.QualityHistology.com RUSHABH Instruments, LLC 1750A Costner Drive Warrington, PA 18976 Phone: 215-491-0081 Fax: 215-491-0080 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030825/76ff7b5e/attachment.htm From wecare <@t> qualityhistology.com Mon Aug 25 14:50:32 2003 From: wecare <@t> qualityhistology.com (wecare@qualityhistology.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Recommendation for Service organizations Message-ID: <002601c36b42$257d7b60$0efea8c0@internetconnect.net> Dear Histonetters, We design and manufacture medical products. We just introduced our new Tissue Embedding Center and are about to introduce H & E Slide Stainer in the market. Could you please recommend your instrument service organizations and contact person? We welcome both positive and negative feedback to ensure that we select proper organizations to provide the best support to you for our products. We thank you for your assistance in advance. Preyas Shah www.QualityHistology.com RUSHABH Instruments, LLC 1750A Costner Drive Warrington, PA 18976 Phone: 215-491-0081 Fax: 215-491-0080 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030825/e3c7e162/attachment.htm From dmccaig <@t> ckha.on.ca Mon Aug 25 15:17:16 2003 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Canadian workload Message-ID: Does anyone have an excel spreadsheet they use to tabulate monthly units for Canadian workload (manually) that they would be willing to share? Thanking you in advance. Diana From dmccaig <@t> ckha.on.ca Mon Aug 25 15:18:50 2003 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] paraffin block storage boxes Message-ID: Does anyone know where I can access cardboard boxes for block storage. I used them years ago and they were about 30 inches long and held about 6 rows of blocks with thin cardboard dividers. Thanking you in advance Diana From Dboyd <@t> swmedctr.com Mon Aug 25 15:34:47 2003 From: Dboyd <@t> swmedctr.com (Julian-Boyd, Dawn) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] NEW PROCESSORS Message-ID: HI! IS ANYONE USING THE NEW (SAKURA) TISSUE TEK CONTINUOUS RAPID PROCESSOR OR THE AUTO TEC EMBEDDING SYSTEM? I'M LOOKING FOR ANY INFO ON THESE TWO ITEMS. THANKS IN ADVANCE. From arme <@t> optonline.net Tue Aug 26 02:13:17 2003 From: arme <@t> optonline.net (Mark Sofferman) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] For sale In-Reply-To: <20030825170001.25651.11124.Mailman@swlx167.swmed.edu> Message-ID: Jung Frigocut 2800N - must know this morning if you want it. Grossing Station - Jewett - very nice condition. ARME - 201-833-1550 arme@optinline.net From Mandy <@t> serotec.co.uk Tue Aug 26 04:25:28 2003 From: Mandy <@t> serotec.co.uk (Mandy Townsend) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Formalin blues Message-ID: Serotec are able to supply an antibody against human CD31 that is suitable for IHC on paraffin sections, requiring HIER with Citrate, clone number 1A10. This is also available in a smaller sample size for evaluation. Please contact me if you require any further information. Mandy Mandy Townsend MSc Technical Services Supervisor Serotec Ltd 22 Bankside Station Approach Kidlington Oxfordshire OX5 1JE Tel: +44 1865 852736 Fax: +44 1865 852739 email: mandy@serotec.co.uk URL: www.serotec.com Serotec-Your first choice for antibodies! IMPORTANT NOTICE: This message and any attachments may be confidential. If this has been sent to you in error, please contact the sender as soon as possible. Serotec Ltd. Registered in England No.1604642 Registered Office: Boswell House, 1-5 Broad Street, Oxford, OX1 SAW. UK -----Original Message----- From: Christian Franci [mailto:cfranci@rigel.com] Sent: Monday, August 25, 2003 6:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin blues I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ This e-mail has been scanned for all viruses by Star Internet. The service is powered by MessageLabs. For more information on a proactive anti-virus service working around the clock, around the globe, visit: http://www.star.net.uk ________________________________________________________________________ From ASelf <@t> gmhsc.com Tue Aug 26 04:42:20 2003 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] test Message-ID: Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From funderwood <@t> mcohio.org Tue Aug 26 05:56:37 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] paraffin block storage boxes Message-ID: Diana, Fisher Scientific sells those and the cardboard slide files. 1.800.766.7000 www.fishersci.com Fred -----Original Message----- From: Diana McCaig Sent: Monday, August 25, 2003 4:19 PM To: "'histonet@lists.utsouthwestern.edu'" Subject: [Histonet] paraffin block storage boxes Does anyone know where I can access cardboard boxes for block storage. I used them years ago and they were about 30 inches long and held about 6 rows of blocks with thin cardboard dividers. Thanking you in advance Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Tue Aug 26 06:36:45 2003 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] EPO Message-ID: <5.2.1.1.2.20030826123348.00a15510@udcf.gla.ac.uk> Eosinophil peroxidase. EPO (L-20) and (S-20) although recommended for WB and Elisa (Santa Cruz Biotechnology) has anyone used them for IHC? Ian. Dr. Ian Montgomery, Histotechnology, Graham Kerr Building, Institute of Biomedical & Life Sciences, University of Glasgow, Glasgow, G12 8QQ. Tel: 0141 339 8855 Office: 4652 Lab: 6644. Pager: 07625 702883 e-mail: ian.montgomery@bio.gla.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/f32c7761/attachment.htm From Lynne.Bell <@t> hitchcock.org Tue Aug 26 07:43:58 2003 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] paraffin block storage boxes Message-ID: Diana, I know this sounds crazy, but, for the past 10 years we have been using pizza boxes from Pizza Hut for our paraffin block storage. You can fit eleven rows across. They are made of strong cardboard. We get them free from Pizza Hut - we just ask for 10 at a time - and we are sure to frequent their restaurant as payment for their generosity. Lynne A. Bell, HT (ASCP) Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4122 From c.m.vanderloos <@t> amc.uva.nl Tue Aug 26 08:11:32 2003 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] RE: IHC- using IgM vs. IgG Message-ID: <9e44c79e0f9d.9e0f9d9e44c7@amc.uva.nl> Dear Majid, To my knowledge secondary antibodies aren't just anti-IgG specific rather than "anti-immunoglobulins" specific. This includes that whenever a vendor uses the term "anti-immunoglobulin" with respect to a secondary antibody reagent (no matter the label) there should be a specificity against the heavy + light chain of all immunoglobulin isotypes IgG, IgM, etc. of the indicated species. Unless stated by the vendor that a secondary antibody is for example anti-IgG heavy chain specific. In those cases there will be no reaction with a mouse IgM primary antibody. So far I failed in finding some literature references about this. Somebody else perhaps? I would like to have it too.... Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center H0-120 Meibergdreef 9 NL-1105 AZ Amsterdam - The Netherlands >From: Majid Ghoddusi >To: "'histonet@lists.utsouthwestern.edu'" >Date: Mon, 25 Aug 2003 17:21:26 +1000 >Subject: [Histonet] IHC- using IgM vs. IgG > >Hello dear histonetters > >Just a very basic question for IHC experts: >if your primary antibody is a mouse IgM, by using a secondary anti- >mouse IgG are you running any risk of getting artefactual signals? >Thanks very much in advance for any help and advice including pointing >to a reference that might discuss this in detail. > >Majid Ghoddusi >Children's Medical Research Institute >Locked Bag 23, Wentworthville NSW 2145 From TMcNemar <@t> lmhealth.org Tue Aug 26 08:23:58 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Sentinel nodes... Message-ID: <90092A4ED388D7119575006008F7112049CB29@NT_EXCHANGE> Hello all, I apologize upfront because I know that this has probably been discussed a great deal but I can't seem to get any information out of the archives..... What precautions do you take in Histology/Pathology for sentinel node biopsies? Any concerns for pregnancy? The information that I have is years old but it says that: Specimens should be transported in lead lined container Biopsies should remain in the lead lined container for least 8 hours (large specimens longer) Like I said, this is old and I'm sure out of date. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/e032124a/attachment.htm From dskaggs <@t> mindspring.com Tue Aug 26 08:28:00 2003 From: dskaggs <@t> mindspring.com (Don Skaggs) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter Message-ID: <3F4B6060.5000107@mindspring.com> Hi, Histonetters! I'm Don Skaggs. You might remember me from such Histology News articles as "How to Not Get Asphyxiated" and "Honey, I Shrunk the Biopsy". Anyway, I have another question from a lab and wanted to give an intelligent answer, so I thought I'd ask you guys: They are looking for a stain for Helicobacter; they have tried a Wright-Giemsa, a Diff-Quick (Isn't that sort of the same thing?) and something else called a "Little Quicker", but to no avail. Any Ideas? Thanks in advance, Don Skaggs Histology News A Newsletter of Histology Information, Innovations and Shameless Promotion From Marilyn.Wadsworth <@t> uvm.edu Tue Aug 26 08:31:30 2003 From: Marilyn.Wadsworth <@t> uvm.edu (Wadsworth, Marilyn P) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] processing mouse lung for cryostat sectioning Message-ID: <92FA977E2471094E8A2B699738A0C8090AD183@med04.med.uvm> I am looking for information relating to the preparation of inflated mouse lung to be used for cryostat sectioning. Currently, the protocol being used uses PBS to inflate the lungs which are subsequently tied off and embedded in TBS or OCT and snap frozen in 2-methylbutane cooled in liquid nitrogen. These lung samples need to be unfixed and are being sectioned at 8 microns with disposable knives at a cryostat temperature of -32C. Any warmer and the TBS or OCT sections, but the lung doesn't. I am suspecting that the PBS inflation may be playing into this - has anyone inflated with agarose, sucrose (or something else) and sectioned successfully? Other ideas? With appreciation... Marilyn Wadsworth Marilyn Wadsworth Microscopy Imaging Center University of Vermont From rbruggeman <@t> psu.edu Tue Aug 26 08:55:06 2003 From: rbruggeman <@t> psu.edu (Richard Bruggeman) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Looking for Roommate for NSH Convention Message-ID: I will be attending this year's NSH convention in Louisville and was wondering if anyone would like to split the cost of a room. If interested, email me directly with the dates you will be in town. Non-smokers only please. Richard "Trey" Bruggeman Milton S. Hershey Medical Center Penn State College of Medicine Department of Pathology, H179 500 University Drive Hershey, PA 17033 (717) 531-1044 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/c6e59158/attachment.htm From la.sebree <@t> hosp.wisc.edu Tue Aug 26 08:57:02 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter Message-ID: I queried our histology section and was told they use Alcian yellow. They used to use Steiner but it got too expensive. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Don Skaggs [mailto:dskaggs@mindspring.com] Sent: Tuesday, August 26, 2003 8:28 AM To: HistoNet Server Subject: [Histonet] Staining for Helicobacter Hi, Histonetters! I'm Don Skaggs. You might remember me from such Histology News articles as "How to Not Get Asphyxiated" and "Honey, I Shrunk the Biopsy". Anyway, I have another question from a lab and wanted to give an intelligent answer, so I thought I'd ask you guys: They are looking for a stain for Helicobacter; they have tried a Wright-Giemsa, a Diff-Quick (Isn't that sort of the same thing?) and something else called a "Little Quicker", but to no avail. Any Ideas? Thanks in advance, Don Skaggs Histology News A Newsletter of Histology Information, Innovations and Shameless Promotion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue Aug 26 09:04:58 2003 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter Message-ID: <90092A4ED388D7119575006008F7112049CB2A@NT_EXCHANGE> Don, We used to to do Warthin Starry. Tride Giemsa, Diff-Quick, and one or two others. We have been doing H. Pylori by IHC for a few years now. So much better. Tom Mc Nemar HT(ASCP) Histology Supervisor Licking Memorial Hospital Newark, Ohio -----Original Message----- From: Don Skaggs [mailto:dskaggs@mindspring.com] Sent: Tuesday, August 26, 2003 9:28 AM To: HistoNet Server Subject: [Histonet] Staining for Helicobacter Hi, Histonetters! I'm Don Skaggs. You might remember me from such Histology News articles as "How to Not Get Asphyxiated" and "Honey, I Shrunk the Biopsy". Anyway, I have another question from a lab and wanted to give an intelligent answer, so I thought I'd ask you guys: They are looking for a stain for Helicobacter; they have tried a Wright-Giemsa, a Diff-Quick (Isn't that sort of the same thing?) and something else called a "Little Quicker", but to no avail. Any Ideas? Thanks in advance, Don Skaggs Histology News A Newsletter of Histology Information, Innovations and Shameless Promotion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmaddox <@t> umich.edu Tue Aug 26 09:20:44 2003 From: rmaddox <@t> umich.edu (Rachel D Maddox) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] unsubscribe Message-ID: <000501c36bdd$3ced1260$709cd38d@DTRRACHEL> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/b0ec94dd/attachment.htm From AMakowsk <@t> med.miami.edu Tue Aug 26 09:24:15 2003 From: AMakowsk <@t> med.miami.edu (Makowski, Anna Lena) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] unsubscribe Message-ID: <3132EBE854CF5B4AA15F9BD2F6781C8D06011605@medex09.med.miami.edu> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/0b0a1228/attachment.htm From philip.bergin <@t> microbio.gu.se Tue Aug 26 09:30:32 2003 From: philip.bergin <@t> microbio.gu.se (Phil Bergin) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter In-Reply-To: <3F4B6060.5000107@mindspring.com> Message-ID: <000501c36bde$9b6ed6b0$a660f182@philb> We use Periodic acid Schiff with toludine blue- works great with helicobacter felis (pylori too I guess). Phil ----------------------------------------------------------------- Philip Bergin G?teborg University Department of Medical Microbiology and Immunology Box 435, SE-405 30 G?teborg, Sweden Phone: +46-31-3424471 Fax: +46-31-826976 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Don Skaggs Sent: den 26 augusti 2003 15:28 To: HistoNet Server Subject: [Histonet] Staining for Helicobacter Hi, Histonetters! I'm Don Skaggs. You might remember me from such Histology News articles as "How to Not Get Asphyxiated" and "Honey, I Shrunk the Biopsy". Anyway, I have another question from a lab and wanted to give an intelligent answer, so I thought I'd ask you guys: They are looking for a stain for Helicobacter; they have tried a Wright-Giemsa, a Diff-Quick (Isn't that sort of the same thing?) and something else called a "Little Quicker", but to no avail. Any Ideas? Thanks in advance, Don Skaggs Histology News A Newsletter of Histology Information, Innovations and Shameless Promotion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mike_LaFriniere <@t> memorial.org Tue Aug 26 09:30:24 2003 From: Mike_LaFriniere <@t> memorial.org (LaFriniere, Mike) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter Message-ID: Hi Don, There are many test for Helicobacter, the two you mentioned as well as the alcian yellow, Stiener, and I'm sure others. However, I had read many people demonstrating higher sensitivity using immunohistochemistry methods with for identification of Helicobacter. Our laboratory wanted to prove this theory. After staining over 100 GI biopsies in our laboratory with Giemsa, Stiener and Immunohistochemistry simultaneously to demonstrate which would be best for the patients we diagnose, our results demonstrated by a far margin that the immunohistochemistry was the method demonstrating the highest sensitivity. In short we were definitely missing staining of Helicobacter using non immunohistochemistry methods. We also found that the antibody through Cell Marque best fit our expectations. One of my concerns was this is definitely a higher cost of staining, however if it was my biopsy or one of my family members, I would want the best method availble to confirm a diagnosis, and not having a disease process possibly missed by low cost less sensitive testing methods. Regards, Michael LaFriniere PA, HT(ASCP) NSH Region III Director Pathology Manager Memorial Hospital Chattanooga TN -----Original Message----- From: Don Skaggs [mailto:dskaggs@mindspring.com] Sent: Tuesday, August 26, 2003 9:28 AM To: HistoNet Server Subject: [Histonet] Staining for Helicobacter Hi, Histonetters! I'm Don Skaggs. You might remember me from such Histology News articles as "How to Not Get Asphyxiated" and "Honey, I Shrunk the Biopsy". Anyway, I have another question from a lab and wanted to give an intelligent answer, so I thought I'd ask you guys: They are looking for a stain for Helicobacter; they have tried a Wright-Giemsa, a Diff-Quick (Isn't that sort of the same thing?) and something else called a "Little Quicker", but to no avail. Any Ideas? Thanks in advance, Don Skaggs Histology News A Newsletter of Histology Information, Innovations and Shameless Promotion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Tue Aug 26 09:57:07 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter In-Reply-To: References: Message-ID: At 10:30 AM -0400 8/26/03, LaFriniere, Mike wrote: >One of my concerns was this >is definitely a higher cost of staining, however if it was my biopsy or one >of my family members, I would want the best method availble to confirm a >diagnosis, and not having a disease process possibly missed by low cost less >sensitive testing methods. If it were me or my family and the clinical context were appropriate, I would want treatment whether the little buggers were demonstrated or not. Personally, I think IHC is overkill for H. pylori and also think the pattern of inflammation more important than actually demonstrating the bugs. BB -- ______________ Bill Blank, MD Heartland Lab, Inc From ASelf <@t> gmhsc.com Tue Aug 26 10:19:44 2003 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] extra-departmental charges Message-ID: Histonetters, How are those of you that do extra-departmental consultations handling them, as far as, charging, accessioning - I guess maybe the whole nine yards? We don't normally do consultations from other hospitals, but here lately we have been getting quite a few. Any in-put on this would be appreciated.... Thanks, Amy Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From la.sebree <@t> hosp.wisc.edu Tue Aug 26 10:35:53 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] extra-departmental charges Message-ID: Amy, We accession them with our own number and any stains done are billed just like an in-house case. A surgical pathology report is issued. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Amy Self [mailto:ASelf@gmhsc.com] Sent: Tuesday, August 26, 2003 10:20 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] extra-departmental charges Histonetters, How are those of you that do extra-departmental consultations handling them, as far as, charging, accessioning - I guess maybe the whole nine yards? We don't normally do consultations from other hospitals, but here lately we have been getting quite a few. Any in-put on this would be appreciated.... Thanks, Amy Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Tue Aug 26 11:03:02 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] RE: IHC- using IgM vs. IgG Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C41111A8@usca0082k03.rallansci.apogent.com> Chris, Because the vast majority of primary antibodies used are IgG, most labeled secondary antibody kits are developed to detect IgG (for instance, the Lab Vision kits note that they detect "IgG's). Unless whole serum is used as a detection reagent there are not going to be significant amounts of antibodies against other Ig fractions. Unfortunately, the ambiguous (and historical) term "immunoglobulins" is often used to describe the IgG fraction rather than being more specific. Tim Morken Product Development Lab Vision / NeoMarkers 47790 Westinghouse Dr Fremont, CA 94539 PH: 510-991-2840 www.labvision.com -----Original Message----- From: C.M. van der Loos [mailto:c.m.vanderloos@amc.uva.nl] Sent: Tuesday, August 26, 2003 6:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC- using IgM vs. IgG Dear Majid, To my knowledge secondary antibodies aren't just anti-IgG specific rather than "anti-immunoglobulins" specific. This includes that whenever a vendor uses the term "anti-immunoglobulin" with respect to a secondary antibody reagent (no matter the label) there should be a specificity against the heavy + light chain of all immunoglobulin isotypes IgG, IgM, etc. of the indicated species. Unless stated by the vendor that a secondary antibody is for example anti-IgG heavy chain specific. In those cases there will be no reaction with a mouse IgM primary antibody. So far I failed in finding some literature references about this. Somebody else perhaps? I would like to have it too.... Chris van der Loos Dept. of Cardiovascular Pathology Academical Medical Center H0-120 Meibergdreef 9 NL-1105 AZ Amsterdam - The Netherlands >From: Majid Ghoddusi >To: "'histonet@lists.utsouthwestern.edu'" >Date: Mon, 25 Aug 2003 17:21:26 +1000 >Subject: [Histonet] IHC- using IgM vs. IgG > >Hello dear histonetters > >Just a very basic question for IHC experts: >if your primary antibody is a mouse IgM, by using a secondary anti- >mouse IgG are you running any risk of getting artefactual signals? >Thanks very much in advance for any help and advice including pointing >to a reference that might discuss this in detail. > >Majid Ghoddusi >Children's Medical Research Institute >Locked Bag 23, Wentworthville NSW 2145 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pedro.louro <@t> spcorp.com Tue Aug 26 11:09:56 2003 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] eye fixatives? Message-ID: <4508920F80C0D411B90200508BF9A9F4030A508C@LAFMSG30.us.schp.com> I'm looking for any feedback on fixation of eyes (small and large animal). More specifically: a fixative that would work well for Histology and EM. Any info. on: * Glutaraldehyde * Karnovsky's Fixative * McDowell - Trump's Fixative or any other fixative would be greatly appreciated. Thanks in advance, Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/ed7b3588/attachment.htm From Cathy.Crumpton <@t> tuality.org Tue Aug 26 12:31:03 2003 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Re: Histonet digest, Vol 1 #25 - 30 msgs Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/4ca699bb/attachment.htm From gcallis <@t> montana.edu Tue Aug 26 13:19:33 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Cryomicrotomy on mouse lung - long reply Message-ID: <3.0.6.32.20030826121933.00b4b828@gemini.msu.montana.edu> You wrote: I am looking for information relating to the preparation of inflated mouse lung to be used for cryostat sectioning. Currently, the protocol being used uses PBS to inflate the lungs which are subsequently tied off and embedded in TBS or OCT and snap frozen in 2-methylbutane cooled in liquid nitrogen. These lung samples need to be unfixed and are being sectioned at 8 microns with disposable knives at a cryostat temperature of -32C. Any warmer and the TBS or OCT sections, but the lung doesn't. I am suspecting that the PBS inflation may be playing into this - has anyone inflated with agarose, sucrose (or something else) and sectioned successfully? Other ideas? With appreciation... Marilyn Wadsworth Marilyn, You cannot inflate fresh murine lung with aqueous solutions, you end up cutting ice. Sucrose cryoprotection is commonly used for NBF prefixed lungs - we use a simple OCT inflation of lung. Fill a 3 ml syringe with 2 1/2 mls OCT, on a dulled 18 guage needle - a diameter that is perfect size for mouse trachea. Dull needle with a fingernail file that has grinding sandpaper, we buy these that have 4 grits, are blue and pink in color at local WalMart. You can leave a bit of bevel on needle - inserts easier with bevel facing up, and you can see it. We reuse these needles rather than reinventing the wheel. Euthanize mouse appropriately, using blunt/sharp scissors, open abdominal cavity, and severe major blood vessels located next to spinal column to bleed out mouse. It is nicer to put a PBS dampened gauze to soak up blood. Detach liver, and open chest cavity carefully with blunt end of scissors under ribs and do it cut lung. Do NOT remove heart at this time. Open rib cage just about the trachea, do NOT cut the trachea, but you must cut through bone above heart. Trachea is the route for inflating lung with OCT. Trachea is exposed, and free from surrounding fascia without cutting trachea. This can be done with a blunt probe or forceps that DOES NOT HAVE A SHARP TIP, run it under trachea to free it from surrounding muscle, etc. You can raise the trachea using applicator sticks or a blunt probe, even fine eye forceps, mouse should be pinned firmly at nose, tail and legs (we use syringe needles for pinning). Using an extremely fine tipped scissors (we buy German steel cuticle scissors from Target or WalMart, they are SUPERB and CHEAP with finer tips than vendors sell!), you cut a tiny "V" shaped cut in TOP of trachea. If you cut across it will retract into lung area and you cannot capture it. Have a small lightweight fine tipped mosquito hemostat forceps (SP?) ready for final dissection. Carefully, keep OCT filled syringe with dull needle flat, insert into v shaped cut, watch it slide into trachea lumen with BEVEL SIDE UP, you can push it towards lung but not too far, then fill lung slowly with OCT. Use a gentle touch, and do not force the needle or syringe plunger. You do not want to push needle through tracheal wall or into lung. If you use more than 2 1/2 mls OCT, you will blow the alveoli to bits, terrible sections. To remove needle, slowly pull it out and quickly clamp trachea with mosquito hemostat forceps just below tiny cut, keeps OCT from backwash, maintains OCT in lung. Gently lift and using fine tip scissors finish dissecting lung out, at this point, you can remove heart (know where it is attached to not nick the filled lung). Embed lung in a Tissue Tek Cryomold with OCT, large size mold, and snap freeze with dry ice/isopentane slurry. WE have had no success with any other cryomold - Tissue Tek has thinner plastic excellent for snap freezing. Holding one long tab with forceps, lower bottom of block into top of dry ice/isopentane (make sure the dry ice level is high in a plastic sample cup, work in a hood. Watch the bottom begin to freeze, then let mold/tissue/OCT sink into slurry. It takes approx 10 seconds to freeze. Liq Nitrogen cooled isopentane will crack OCT/block, too cold. Remove and let isopentane evaporate, mount block on chuck, and cut at -20C, colder is not necessary with OCT filled lung, in fact, creates crunchy sections if TOO cold. We cut at 5 um, no problems and have perfect sections on whole OCT filled lung. We use disposable high profile blades, Accuedge are a preference, and brush technic - antiroll device resides in a drawer. To get away from isopentane, float a plastic petri dish on liquid nitrogen using a platform in N2 to support dish, but do not let N2 go into dish, it must float on N2 and surround dish. Set embedded lung in dish and let it freeze, no artifact, perfect sections. If you need help with snap freezing setup, I will discuss that privately. We trim plastic edges from 3 sides of cryomold so we can freeze faster and store blocks in 50 ml centrifuge tubes at -80C. There are other snap freezing methods that work, but liquid N2/isopentane requires too much recooling when you have 20 mice lined up for dissection, etc. We never use this method for a large experimental tissue collection. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu From Bonnie.P.Whitaker <@t> uth.tmc.edu Tue Aug 26 13:28:30 2003 From: Bonnie.P.Whitaker <@t> uth.tmc.edu (Bonnie P Whitaker) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter In-Reply-To: Message-ID: Hi Everyone! Let my add my $0.02 worth. In my lab we routinely run IHC using automation, and doing a handful of slides more or less won't make much difference in terms of tech time. We don't have any automation for specials and we're stretched pretty thin some days. If someone has to make up reagents and do a special for H. pylori, we really feel that with all things considered, the immuno is the easiest, cheapest to run (if you look at the cost per test vs. what we charge per test) and the most sensitive. For us that's a win/win/win situation! Bonnie Whitaker Technical Director, Histology Laboratory Department of Pathology and Laboratory Medicine UT Med School 6431 Fannin MSB 2.231 Houston, TX 77030 713-500-6792 -----Original Message----- From: histonet-admin@lists.utsouthwestern.edu [mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of LaFriniere, Mike Sent: Tuesday, August 26, 2003 9:30 AM To: 'dskaggs@mindspring.com'; HistoNet Server Subject: RE: [Histonet] Staining for Helicobacter Hi Don, There are many test for Helicobacter, the two you mentioned as well as the alcian yellow, Stiener, and I'm sure others. However, I had read many people demonstrating higher sensitivity using immunohistochemistry methods with for identification of Helicobacter. Our laboratory wanted to prove this theory. After staining over 100 GI biopsies in our laboratory with Giemsa, Stiener and Immunohistochemistry simultaneously to demonstrate which would be best for the patients we diagnose, our results demonstrated by a far margin that the immunohistochemistry was the method demonstrating the highest sensitivity. In short we were definitely missing staining of Helicobacter using non immunohistochemistry methods. We also found that the antibody through Cell Marque best fit our expectations. One of my concerns was this is definitely a higher cost of staining, however if it was my biopsy or one of my family members, I would want the best method availble to confirm a diagnosis, and not having a disease process possibly missed by low cost less sensitive testing methods. Regards, Michael LaFriniere PA, HT(ASCP) NSH Region III Director Pathology Manager Memorial Hospital Chattanooga TN -----Original Message----- From: Don Skaggs [mailto:dskaggs@mindspring.com] Sent: Tuesday, August 26, 2003 9:28 AM To: HistoNet Server Subject: [Histonet] Staining for Helicobacter Hi, Histonetters! I'm Don Skaggs. You might remember me from such Histology News articles as "How to Not Get Asphyxiated" and "Honey, I Shrunk the Biopsy". Anyway, I have another question from a lab and wanted to give an intelligent answer, so I thought I'd ask you guys: They are looking for a stain for Helicobacter; they have tried a Wright-Giemsa, a Diff-Quick (Isn't that sort of the same thing?) and something else called a "Little Quicker", but to no avail. Any Ideas? Thanks in advance, Don Skaggs Histology News A Newsletter of Histology Information, Innovations and Shameless Promotion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lindachristensen520 <@t> msn.com Tue Aug 26 14:41:54 2003 From: lindachristensen520 <@t> msn.com (Linda Christensen) Date: Fri Sep 16 15:21:47 2005 Subject: [histonet] unsubscribe Message-ID: Linda Charles Christensen _________________________________________________________________ MSN 8: Get 6 months for $9.95/month. http://join.msn.com/?page=dept/dialup From GREYTRUNK <@t> aol.com Tue Aug 26 15:05:26 2003 From: GREYTRUNK <@t> aol.com (GREYTRUNK@aol.com) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter Message-ID: <109.26e51130.2c7d1786@aol.com> There is an H Pylori stain that takes 2 minutes. It is called thiazine. Roxanne -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/499ed820/attachment.htm From bill501 <@t> mindspring.com Tue Aug 26 15:24:09 2003 From: bill501 <@t> mindspring.com (Bill Blank) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Staining for Helicobacter In-Reply-To: References: Message-ID: At 1:28 PM -0500 8/26/03, Bonnie P Whitaker wrote: >we >really feel that with all things considered, the immuno is the easiest, >cheapest to run (if you look at the cost per test vs. what we charge per >test) and the most sensitive. For us that's a win/win/win situation! What is the reimbursement by Medicare and medicaid for an IHC vs an Alcian yellow, eg. -- _____________________________ Bill Blank http://kernunnos.com (Celtic studies and numismatics) From gcallis <@t> montana.edu Tue Aug 26 17:32:13 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:47 2005 Subject: [Histonet] Cryomicrotomy on mouse lung - long reply In-Reply-To: <001801c36c1a$ae835d20$a485080a@wsahs.nsw.gov.au> References: <3.0.6.32.20030826121933.00b4b828@gemini.msu.montana.edu> Message-ID: <3.0.6.32.20030826163213.00b4d160@gemini.msu.montana.edu> Bill, Yes, we have diluted OCT with PBS 1:1, but found it was not necessary. Lung filled nicely plus diluted OCT is more aqueous, looks funny at times when frozen as water concentration increases. If it was any more dilute, sectioning was poorer. We had good sectioning with 1:1 diluted OCT, but it also tends to diffuse out of lung - I had to work quickly. We don't use TBS embedding media (if it isn't broken, don't fix it attitude) and are finding some brands don't do the job as well as OCT, but that is a preference plus application usage (filling small intestine lumen with OCT is also done after rinsing feces out with PBS, sort of the acid test of cryoembedding media for us). People really need to try different cryomedias, note plural. We discovered some do not hold tissue as well resulting in sections compressed or cryoembedding media pulling away from villi inside murine intestine. It pays to try them all just to see if one works better with rodents, animal tissues. Many of these media are developed and tested in clinical/human situations and then the "mousie" types come along and do something entirely different, testing cryoembedding media to their limits. This doesn't mean a product is inferior, just not best for us. I have tried Shandons colored media in lung and it was so neat to see a colored goo inflate the lung i.e. green or blue, theirs also worked well with lung. Not tried Shandon's with intestine though. So advice is if you get poor sections with one cryomedia, try others - vendors graciously send samples. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) email: gcallis@montana.edu At 07:40 AM 8/27/2003 +1000, you wrote: > >Gayle, > >Have you tried diluting the TBS or OCT mountant? > >Bill Sinai >Laboratory Manager >Tissue Pathology >ICPMR >P.O. Box 533 >Wentworthville NSW 2145 >Ph 02 9845 7774 >----- Original Message ----- >From: "Gayle Callis" >To: >Sent: Wednesday, August 27, 2003 4:19 AM >Subject: [Histonet] Cryomicrotomy on mouse lung - long reply > > >> You wrote: >> >> I am looking for information relating to the preparation of inflated mouse >> lung to be used for cryostat sectioning. Currently, the protocol being >used >> uses PBS to inflate the lungs which are subsequently tied off and embedded >> in TBS or OCT and snap frozen in 2-methylbutane cooled in liquid nitrogen. >> These lung samples need to be unfixed and are being sectioned at 8 microns >> with disposable knives at a cryostat temperature of -32C. Any warmer and >> the TBS or OCT sections, but the lung doesn't. I am suspecting that the >PBS >> inflation may be playing into this - has anyone inflated with agarose, >> sucrose (or something else) and sectioned successfully? Other ideas? With >> appreciation... >> >> Marilyn Wadsworth >> >> Marilyn, >> >> You cannot inflate fresh murine lung with aqueous solutions, you end up >> cutting ice. Sucrose cryoprotection is commonly used for NBF prefixed >> lungs - we use a simple OCT inflation of lung. >> >> Fill a 3 ml syringe with 2 1/2 mls OCT, on a dulled 18 guage needle - a >> diameter that is perfect size for mouse trachea. Dull needle with a >> fingernail file that has grinding sandpaper, we buy these that have 4 >> grits, are blue and pink in color at local WalMart. You can leave a bit >of >> bevel on needle - inserts easier with bevel facing up, and you can see it. >> We reuse these needles rather than reinventing the wheel. >> >> Euthanize mouse appropriately, using blunt/sharp scissors, open abdominal >> cavity, and severe major blood vessels located next to spinal column to >> bleed out mouse. It is nicer to put a PBS dampened gauze to soak up >blood. >> Detach liver, and open chest cavity carefully with blunt end of scissors >> under ribs and do it cut lung. Do NOT remove heart at this time. Open rib >> cage just about the trachea, do NOT cut the trachea, but you must cut >> through bone above heart. Trachea is the route for inflating lung with >OCT. >> Trachea is exposed, and free from surrounding fascia without cutting >> trachea. This can be done with a blunt probe or forceps that DOES NOT >HAVE >> A SHARP TIP, run it under trachea to free it from surrounding muscle, etc. >> >> You can raise the trachea using applicator sticks or a blunt probe, even >> fine eye forceps, mouse should be pinned firmly at nose, tail and legs >(we >> use syringe needles for pinning). Using an extremely fine tipped scissors >> (we buy German steel cuticle scissors from Target or WalMart, they are >> SUPERB and CHEAP with finer tips than vendors sell!), you cut a tiny "V" >> shaped cut in TOP of trachea. If you cut across it will retract into lung >> area and you cannot capture it. Have a small lightweight fine tipped >> mosquito hemostat forceps (SP?) ready for final dissection. >> >> Carefully, keep OCT filled syringe with dull needle flat, insert into v >> shaped cut, watch it slide into trachea lumen with BEVEL SIDE UP, you can >> push it towards lung but not too far, then fill lung slowly with OCT. Use >> a gentle touch, and do not force the needle or syringe plunger. You do >not >> want to push needle through tracheal wall or into lung. If you use more >> than 2 1/2 mls OCT, you will blow the alveoli to bits, terrible sections. >> To remove needle, slowly pull it out and quickly clamp trachea with >> mosquito hemostat forceps just below tiny cut, keeps OCT from backwash, >> maintains OCT in lung. Gently lift and using fine tip scissors finish >> dissecting lung out, at this point, you can remove heart (know where it is >> attached to not nick the filled lung). >> >> Embed lung in a Tissue Tek Cryomold with OCT, large size mold, and snap >> freeze with dry ice/isopentane slurry. WE have had no success with any >> other cryomold - Tissue Tek has thinner plastic excellent for snap >> freezing. Holding one long tab with forceps, lower bottom of block into >top >> of dry ice/isopentane (make sure the dry ice level is high in a plastic >> sample cup, work in a hood. Watch the bottom begin to freeze, then let >> mold/tissue/OCT sink into slurry. It takes approx 10 seconds to freeze. >> Liq Nitrogen cooled isopentane will crack OCT/block, too cold. Remove and >> let isopentane evaporate, mount block on chuck, and cut at -20C, colder is >> not necessary with OCT filled lung, in fact, creates crunchy sections if >> TOO cold. We cut at 5 um, no problems and have perfect sections on whole >> OCT filled lung. We use disposable high profile blades, Accuedge are a >> preference, and brush technic - antiroll device resides in a drawer. >> >> To get away from isopentane, float a plastic petri dish on liquid nitrogen >> using a platform in N2 to support dish, but do not let N2 go into dish, >it >> must float on N2 and surround dish. Set embedded lung in dish and let it >> freeze, no artifact, perfect sections. >> >> If you need help with snap freezing setup, I will discuss that privately. >> We trim plastic edges from 3 sides of cryomold so we can freeze faster and >> store blocks in 50 ml centrifuge tubes at -80C. >> >> There are other snap freezing methods that work, but liquid N2/isopentane >> requires too much recooling when you have 20 mice lined up for dissection, >> etc. We never use this method for a large experimental tissue collection. >> >> >> >> >> >> >> >> >> >> >> >> Gayle Callis >> MT,HT,HTL(ASCP) >> Research Histopathology Supervisor >> Veterinary Molecular Biology - Marsh Lab >> Montana State University - Bozeman >> S. 19th and Lincoln St >> Bozeman MT 59717-3610 >> >> 406 994-6367 (lab with voice mail) >> 406 994-4303 (FAX) >> >> email: gcallis@montana.edu >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >__________________________________________________________________ > >This electronic message and any attachments may be confidential. If you >are not the intended recipient of this message would you please delete the >message and any attachments and advise the sender. Western Sydney >Area Health Services (WSAHS) uses virus scanning software but excludes >any liability for viruses contained in any email or attachment. > >This email may contain privileged and confidential information intended >only for the use of the addressees named above. If you are not the >intended recipient of this email, you are hereby notified that any use, >dissemination, distribution, or reproduction of this email is prohibited. If >you have received this email in error, please notify WSAHS >immediately. > >Any views expressed in this email are those of the individual sender >except where the sender expressly and with authority states them >to be the views of WSAHS. > > > From scooper17 <@t> cfl.rr.com Tue Aug 26 17:34:55 2003 From: scooper17 <@t> cfl.rr.com (Sherrie) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubcribe Message-ID: <011701c36c22$46b34ef0$4c7e2341@SHERRIE> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/3507cfe1/attachment.htm From IBirchall <@t> groupwise.swin.edu.au Tue Aug 26 18:24:21 2003 From: IBirchall <@t> groupwise.swin.edu.au (Ian Birchall) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] processing polymers Message-ID: Does anybody have any helpful hints for processing and paraffin embedding polymers such as poly caprolactone, polyurethanes, polylactide etc. Thanks, Ian Birchall, Melbourne. From pdelvent <@t> wyoming.com Tue Aug 26 19:34:52 2003 From: pdelvent <@t> wyoming.com (Priscilla Delventhal) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] H pylori staining Message-ID: We have been using the #2 stain (blue) from the Diff-Quick stain and tweaked it just a bit. We stain for 5 minutes in the stain and rinse in water. Then 3 short dips in 80% Reagent Alcohol and dehydrate, clear and coverslip. Although they don't stand out and wave a flag, they are recognizable and the reaction to the bug is still an important factor as to whether they are going to cause ulcers. Just my 2 cents-----Priscilla in Central Wyoming. Just one month to retirement then I can do some more traveling. From billions <@t> public1.sz.js.cn Tue Aug 26 19:40:38 2003 From: billions <@t> public1.sz.js.cn (Sinoera Tech) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Re: Alcian Blue Alcian Yellow Alcian Green References: Message-ID: <008b01c36c33$f2721300$2e00a8c0@df> Dear Sirs, We have Alcian Blue, Alcian Yellow and Alcian Green available. Kind Regards. - Minggeng Wang, Ph.D / President SUZHOU SINOERA CHEM CO., LTD. Fax: +86 512 68224995 Tel: +86 512 68246939 From Histolady710 <@t> aol.com Tue Aug 26 20:00:01 2003 From: Histolady710 <@t> aol.com (Histolady710@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] HT Exam Message-ID: I'm studying for the HT exam. Which study material should I concentrate on most - Board of Registry Study Guide, NSH Self-Assessment Examination Publications or Histotechnology, A Self-Assessment Workbook by Frieda Carson? Thanks for your responses ! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030826/44d907d0/attachment.htm From bills <@t> icpmr.wsahs.nsw.gov.au Wed Aug 27 01:19:57 2003 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Ventana Benchmark Message-ID: <008d01c36c63$3cdd3d00$a485080a@wsahs.nsw.gov.au> Hi All, Has anyone out there using the Ventana Benchmark had trouble with the antibody not being dispensed onto the slides? Also have you ahd a similar problem with haematoxylin being missed on some slides? This appears to have developed recently and we are almost certain that it is neither the dispensers nor the reagents. We think the slide carousel may be getting out of sinc with the dispenser carousel, although we don't know how. Many thanks for any replies. Bill Sinai Laboratory Manager Tissue Pathology ICPMR P.O. Box 533 Wentworthville NSW 2145 Ph 02 9845 7774 __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. From Marjorie.Lehman <@t> unilever.com Wed Aug 27 06:38:31 2003 From: Marjorie.Lehman <@t> unilever.com (marjorie lehman) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Histologist position Message-ID: Dear Netters, This has been my job for 42 years, so if you want any more information feel free to contact me. Marjorie.Lehman@unilever.com HISTOLOGIST Edgewater, New Jersey, UNITED STATES SUMMARY: A detail oriented histologist needed for a full time position at Unilever Research and Development in Edgewater, NJ. Degree/Qualification Preferred: BS Majors/Subjects Preferred: Biology Job Description: Unilever Research and Development has an immediate opening for a Histologist to Join our team in Edgewater, NJ. Primary responsibilities will include tissue trimming, processing, freezing, microtome and cryostat sectioning, basic and immuno histochemical staining and imaging (regular and confocal). Expertise in using, maintaining and troubleshooting laboratory equipment including confocal microscope is required. The candidate will provide training in histology to other users and assist in determining appropriate methods of fixation, processing and staining for various research projects. The individual should be able to use image-processing applications for data analysis, should assist in the development of new histochemical assays, prepare technical reports and make scientific presentations. The individual will be responsible for ordering and maintaining an inventory of supplies. Experience in biological electron microscopy (tissue preparation and sectioning) would be a plus. Contact: Manoj.Misra@unilever.com or Fax 201-840-8291 From Jackie.O'Connor <@t> abbott.com Wed Aug 27 07:28:28 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure Message-ID: Fellow histonetters: I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years - so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance. Jackie O'Connor -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/3e058f60/attachment.htm From cfavara <@t> niaid.nih.gov Wed Aug 27 08:34:00 2003 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID)) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure Message-ID: I had this happen repeatedly and eventually found out the rotator was bad on the processor so be aware of further problems. The rotator on my processor would rotate at will and plunk in any basket. To rescue the tissue I would just place back in the solution it was in before suspended in air then proceed with the processing. I would mostly with mouse tissue and samples were readable but we elected to repeat our studies as the morphology was less than perfect and cutting was a nightmare. Not hopeless just difficult! C Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 -----Original Message----- From: Jackie.O'Connor@abbott.com [mailto:Jackie.O'Connor@abbott.com] Sent: Wednesday, August 27, 2003 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor failure Fellow histonetters: I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years - so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance. Jackie O'Connor -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/6109e950/attachment.htm From Snobird75 <@t> aol.com Wed Aug 27 08:59:04 2003 From: Snobird75 <@t> aol.com (Snobird75@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] subscribe digest Message-ID: <37501472.22568A74.02923F89@aol.com> From la.sebree <@t> hosp.wisc.edu Wed Aug 27 09:21:13 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Ventana Benchmark Message-ID: Never had this happen unless someone inadvertently rotated the reagent wheel while the run was under way. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Bill Sinai [mailto:bills@icpmr.wsahs.nsw.gov.au] Sent: Wednesday, August 27, 2003 1:20 AM To: histonet Subject: [Histonet] Ventana Benchmark Hi All, Has anyone out there using the Ventana Benchmark had trouble with the antibody not being dispensed onto the slides? Also have you ahd a similar problem with haematoxylin being missed on some slides? This appears to have developed recently and we are almost certain that it is neither the dispensers nor the reagents. We think the slide carousel may be getting out of sinc with the dispenser carousel, although we don't know how. Many thanks for any replies. Bill Sinai Laboratory Manager Tissue Pathology ICPMR P.O. Box 533 Wentworthville NSW 2145 Ph 02 9845 7774 __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Wed Aug 27 09:22:52 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Ventana Benchmark Message-ID: Yes, it's happened to me twice. As a matter of fact, the service rep. is on his way here right now. The problem seems to be the belt that moves the carousel. Once the belt was replaced on my other benchmark the problem went away, so I'm hoping for the same results on this one. Good luck! Juan C. Gutierrez, HT(ASCP) Histology Supervisor Christus Santa Rosa Healthcare (210)704-2533 -----Original Message----- From: Bill Sinai [mailto:bills@icpmr.wsahs.nsw.gov.au] Sent: Wed 8/27/2003 1:19 AM To: histonet Cc: Subject: [Histonet] Ventana Benchmark Hi All, Has anyone out there using the Ventana Benchmark had trouble with the antibody not being dispensed onto the slides? Also have you ahd a similar problem with haematoxylin being missed on some slides? This appears to have developed recently and we are almost certain that it is neither the dispensers nor the reagents. We think the slide carousel may be getting out of sinc with the dispenser carousel, although we don't know how. Many thanks for any replies. Bill Sinai Laboratory Manager Tissue Pathology ICPMR P.O. Box 533 Wentworthville NSW 2145 Ph 02 9845 7774 __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Western Sydney Area Health Services (WSAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify WSAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of WSAHS. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Wed Aug 27 09:53:55 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Rb-1 IHC staining laboratories Message-ID: Hi histonetters, A pathologist at our institution is looking for a reference lab that does IHC staining for Rb-1. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From STEGTM <@t> samcstl.org Wed Aug 27 10:19:16 2003 From: STEGTM <@t> samcstl.org (Therersa Stegall) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Fe controls?! Message-ID: Hello! If anyone out here could help with some tissue/blocks for Fe controls, we would certainly appreciate it. Seems we've nearly run out of control blocks, and all the available tissue I can lay my hands on it autolyzed! If you can help, please reply, and I'll send you the details..... Peace, Terre From ckbyrne <@t> exelixis.com Wed Aug 27 10:25:16 2003 From: ckbyrne <@t> exelixis.com (Carrie Kyle-Byrne) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] p53 to recommend? Message-ID: <001a01c36caf$6b394da0$860c1dac@ckbyrnewkst> hi all, just wondering what everyone's fav p53 aby is.....i'm looking for one that hits both wild-type and mutant? i've just tried clone PAb122 and i'm not happy with it. thanks for you input..... Carrie Kyle-Byrne, BHS, HT(ASCP) Assoc. Research Scientist II Antibody Core Lab Signal Transduction Research Exelixis, Inc. 170 Harbor Way P.O. Box 511 South San Francisco CA 94083-0511 USA Phone: (1 650) 837-8023 Fax: (1 650) 837-7220 ________________________________________________________________ This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From rstapf <@t> adventisthealthcare.com Wed Aug 27 10:35:24 2003 From: rstapf <@t> adventisthealthcare.com (Ross Stapf) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] p53 to recommend? Message-ID: We use Dako's P53. CAT # M7001. Clone DO-7. Ross Stapf Histology Supervisor Washington Adventist Hospital Takoma Park MD -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/3d5b8af4/attachment.htm From ASelf <@t> gmhsc.com Wed Aug 27 10:56:01 2003 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] microwave procedure for mucin Message-ID: Hello All !!!! Does anyone out there is histoland have a microwave staining procedure for Mayer's Mucicarmine that they would share with me? Thanks, Amy Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Patty.Lott <@t> ORTHO.UAB.EDU Wed Aug 27 11:01:01 2003 From: Patty.Lott <@t> ORTHO.UAB.EDU (Patty Lott) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] solochrome cyanine Message-ID: <85F6C7A1330E794DB8540AFD001CC77E026DF8@rosco.ortho.uab.edu> Does anyone know where to order solochrome cyanine? Patty Lott, Laboratory Supervisor Orthopaedic Research Laboratory Center for Metabolic Bone Disease Laboratory University of Alabama at Birmingham LHRB B37 0007 1919 7th Ave. South Birmingham, AL 35294 (205) 934-2007 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/b2c8b0ba/attachment.htm From LuckG <@t> empirehealth.org Wed Aug 27 11:31:44 2003 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Rb-1 IHC staining laboratories Message-ID: Linda, My recommendation would be Dr. Allen Gown's lab (ie. Phenopath) in Seattle. Contact Phyllis Davie, Clinical Lab Manager, through their website (phenopath.com) or Patty Loykasek, Lead IHC tech (ploykasek@phenopath.com). Good hunting, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 509.473.7077 luckg@empirehealth.org -----Original Message----- From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu] Sent: Wednesday, August 27, 2003 7:54 AM To: Histonet Subject: [Histonet] Rb-1 IHC staining laboratories Hi histonetters, A pathologist at our institution is looking for a reference lab that does IHC staining for Rb-1. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From i_stain <@t> yahoo.com Wed Aug 27 12:13:16 2003 From: i_stain <@t> yahoo.com (Scott Mordue) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] immunostainer-DAB waste Message-ID: <20030827171316.58825.qmail@web42005.mail.yahoo.com> dear all, Is it worth buying an immunostainer that separates out DAB waste? Would like to get opinions as we are looking at our options. thanks Scott CSU __________________________________ Do you Yahoo!? The New Yahoo! Search - Faster. Easier. Bingo. http://search.yahoo.com From swong <@t> rigel.com Wed Aug 27 12:14:04 2003 From: swong <@t> rigel.com (Steve Wong) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Finding a used cryotostat in california Message-ID: <016101c36cbe$9db0edb0$9904000a@SWONGTP> Does anyone have a good suggestion for finding used equipment for sectioning in northern CA? I am mainly interested in setting up for making frozen sections and maybe Formalin blocks as well. Thanks in advance, Steve -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/4ab6a90a/attachment.htm From bryand <@t> netbistro.com Wed Aug 27 12:31:39 2003 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] solochrome cyanine References: <85F6C7A1330E794DB8540AFD001CC77E026DF8@rosco.ortho.uab.edu> Message-ID: <002201c36cc1$13b62d20$9f70c2cf@bryand> It can be obtained from Sigma under the name eriochrome cyanine RC, CI 43820, product # E 2502. Bryan Llewellyn ----- Original Message ----- From: Patty Lott To: 'histonet@lists.utsouthwestern.edu' Sent: Wednesday, August 27, 2003 9:01 AM Subject: [Histonet] solochrome cyanine Does anyone know where to order solochrome cyanine? Patty Lott, Laboratory Supervisor Orthopaedic Research Laboratory Center for Metabolic Bone Disease Laboratory University of Alabama at Birmingham LHRB B37 0007 1919 7th Ave. South Birmingham, AL 35294 (205) 934-2007 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/6105d5c6/attachment.htm From LuckG <@t> empirehealth.org Wed Aug 27 12:39:03 2003 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] immunostainer-DAB waste Message-ID: Scott, We have a DAKO immunostainer which does this and is a distinct advantage. By doing so it reduces the amount of waste that must be disposed of by haz-mat measures thus minimizing disposal costs. It also simplifies compliance and makes separating and shipping the materials for disposal (for us it's xylene "still" waste, paraffin from the tissue processors and DAB) a simple semi-annual affair. Greg Luck Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 509.473.7077 luckg@empirehealth.org -----Original Message----- From: Scott Mordue [mailto:i_stain@yahoo.com] Sent: Wednesday, August 27, 2003 10:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] immunostainer-DAB waste dear all, Is it worth buying an immunostainer that separates out DAB waste? Would like to get opinions as we are looking at our options. thanks Scott CSU __________________________________ Do you Yahoo!? The New Yahoo! Search - Faster. Easier. Bingo. http://search.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Wed Aug 27 12:45:45 2003 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] immunostainer-DAB waste Message-ID: We have Ventana immunostainers and our safety department determined that the amount of DAB in the waste was so miniscule and diluted as to be safe to dispose of down the drain. Linda A. Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-2472 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Scott Mordue [mailto:i_stain@yahoo.com] Sent: Wednesday, August 27, 2003 12:13 PM To: histonet@pathology.swmed.edu Subject: [Histonet] immunostainer-DAB waste dear all, Is it worth buying an immunostainer that separates out DAB waste? Would like to get opinions as we are looking at our options. thanks Scott CSU __________________________________ Do you Yahoo!? The New Yahoo! Search - Faster. Easier. Bingo. http://search.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrae <@t> u.washington.edu Wed Aug 27 12:47:51 2003 From: andrae <@t> u.washington.edu (A. Erickson) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Egg yolk embedding of brain Message-ID: I am trying to locate a copy of the article "Egg Yolk Embedding of Whole Primate Brain Segments" Simmons, Donna M. Journal of Histotechnology, 2:2 June, 1979 pp.62-64. If anyone can help, i will greatly appreciate it. Regards, Andra Erickson Research Technologist, UW WaNPRC, Seattle, Wa From funderwood <@t> mcohio.org Wed Aug 27 12:59:01 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure Message-ID: Hi Jackie. I would recommend rehydrating them in normal saline instead of water. Make certain that the samples are adequately rehydrated before processing again. Fred -----Original Message----- From: Sent: Wednesday, August 27, 2003 8:28 AM To: Subject: [Histonet] Processor failure Fellow histonetters: I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance. Jackie O'Connor From funderwood <@t> mcohio.org Wed Aug 27 12:59:01 2003 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure Message-ID: Hi Jackie. I would recommend rehydrating them in normal saline instead of water. Make certain that the samples are adequately rehydrated before processing again. Fred -----Original Message----- From: Sent: Wednesday, August 27, 2003 8:28 AM To: Subject: [Histonet] Processor failure Fellow histonetters: I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance. Jackie O'Connor From CCLYATT <@t> mail.mcg.edu Wed Aug 27 12:57:36 2003 From: CCLYATT <@t> mail.mcg.edu (Claye Clyatt) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Job Descriptions Message-ID: Per request, I'm posting the only response I've received so for sharing of job descriptions/evaluation forms. Go to uthscsa.edu/hr You will find the info there. I'm sure we could all benefit by sharing this information. If there is anyone else who can add information to this list, many of us would be greatly appreciative. Managing people is the most difficult part of my job. I'm sure there are many people out there with better skills/experience/ideas that can help us all in this area of our jobs. Thanks, Claye Claye Clyatt Chief Histotechnologist Department of Pathology Room #BF119 Medical College of Georgia Augusta, Ga 30912 office (706) 721-3630 pager (706) 721-7243-1132 e-mail: cclyatt@mail.mcg.edu From jlinda <@t> ces.clemson.edu Wed Aug 27 13:07:14 2003 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Re: processing polymers Message-ID: <5.1.0.14.2.20030827135858.05759280@mailhost.ces.clemson.edu> Ian, You wrote: "Does anybody have any helpful hints for processing and paraffin embedding polymers such as poly caprolactone, polyurethanes, polylactide etc. Thanks, Ian Birchall, Melbourne." We do lots of embedding of polymers and have found it depends entirely on the polymer. Some are light, heat, and chemically reactive. Then you have to play "Merlin-the-Magician"! Seriously, are you sectioning beads, scaffolds or gels? I have a PowerPoint handout I can send you if you would be interested? Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From Cathie.Crukley <@t> lhsc.on.ca Wed Aug 27 13:07:18 2003 From: Cathie.Crukley <@t> lhsc.on.ca (Cathie Crukley) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] solochrome cyanine Message-ID: Will this stain still be referred to as a solochrome cyanine stain or should it be referred to as a eriochrome cyanine stain? >>> "Bryan Llewellyn" 08/27/03 01:31PM >>> It can be obtained from Sigma under the name eriochrome cyanine RC, CI 43820, product # E 2502. Bryan Llewellyn ----- Original Message ----- From: Patty Lott To: 'histonet@lists.utsouthwestern.edu' Sent: Wednesday, August 27, 2003 9:01 AM Subject: [Histonet] solochrome cyanine Does anyone know where to order solochrome cyanine? Patty Lott, Laboratory Supervisor Orthopaedic Research Laboratory Center for Metabolic Bone Disease Laboratory University of Alabama at Birmingham LHRB B37 0007 1919 7th Ave. South Birmingham, AL 35294 (205) 934-2007 From mcauliff <@t> umdnj.edu Wed Aug 27 13:38:35 2003 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Egg yolk embedding of brain In-Reply-To: References: Message-ID: <3F4CFAAB.2000000@umdnj.edu> You might also check out: Adoff, L.M. 1981. Egg yolk embedding for frozen whole brain sections. Stain Technology 56(2):125-126. and/or Gurusinghe, C.J. and D. Ehrlich. 1986. Gelatin embedding of central nervous system tissues improves the quality of vibratome sections. Stain Technology 61:324-326. The journal Stain Technology is now known as Biotechnique and Histochemistry. A. Erickson wrote: >I am trying to locate a copy of the article "Egg Yolk Embedding of Whole >Primate Brain Segments" Simmons, Donna M. Journal of Histotechnology, 2:2 >June, 1979 pp.62-64. If anyone can help, i will greatly appreciate it. >Regards, Andra Erickson Research Technologist, UW WaNPRC, Seattle, Wa > > > > Geoff -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/ff689838/attachment.htm From gcallis <@t> montana.edu Wed Aug 27 14:30:33 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] solochrome cyanine In-Reply-To: Message-ID: <3.0.6.32.20030827133033.00b4dea8@gemini.msu.montana.edu> Solochrome cyanine R, and what are you using it for, myelin stain? If so, John Kiernan has publication on this dyes use that is outstanding for either myelin or other purposes. Possibly he is looking in and will give the reference. If not, I will try an locate my copy, moving a lab at this point, everything is buried. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology - Marsh Lab Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 At 02:07 PM 8/27/2003 -0400, you wrote: >Will this stain still be referred to as a solochrome cyanine stain or >should it be referred to as a eriochrome cyanine stain? > >>>> "Bryan Llewellyn" 08/27/03 01:31PM >>> >It can be obtained from Sigma under the name eriochrome cyanine RC, CI >43820, product # E 2502. > >Bryan Llewellyn > ----- Original Message ----- > From: Patty Lott > To: 'histonet@lists.utsouthwestern.edu' > Sent: Wednesday, August 27, 2003 9:01 AM > Subject: [Histonet] solochrome cyanine > > > Does anyone know where to order solochrome cyanine? > > > > Patty Lott, Laboratory Supervisor > > Orthopaedic Research Laboratory > > Center for Metabolic Bone Disease Laboratory > > University of Alabama at Birmingham > > LHRB B37 0007 > > 1919 7th Ave. South > > Birmingham, AL 35294 > > (205) 934-2007 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From micro <@t> superlink.net Wed Aug 27 14:35:41 2003 From: micro <@t> superlink.net (Markus Meyenhofer) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Re: Finding a used cryotostat in california In-Reply-To: <016101c36cbe$9db0edb0$9904000a@SWONGTP> References: <016101c36cbe$9db0edb0$9904000a@SWONGTP> Message-ID: <20030827193541.28636.qmail@mail1.superlink.net> We sell reconditioned Cryostats, Histo-, EM- and Lab Equipment. Please contact me direct for a list and prices. Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 micro@superlink.net Steve Wong writes: > Does anyone have a good suggestion for finding used equipment for sectioning in northern CA? > I am mainly interested in setting up for making frozen sections and maybe Formalin blocks as well. > Thanks in advance, > Steve From tissuearray <@t> hotmail.com Wed Aug 27 14:39:41 2003 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Tissue Array Instruction Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030827/01d7853f/attachment.htm From tvedilago <@t> system1.net Wed Aug 27 14:45:40 2003 From: tvedilago <@t> system1.net (Tommy Vedilago) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Histotechs Wanted Message-ID: We are currently searching for certified, top quality Histotechs for a growing laboratory in Dallas, TX. They are offering excellent pay and benefits but, more importantly, a great place to work with a family of coworkers. They are willing to relocate and are interested in qualified techs from anywhere in the country. I will be happy to discuss this and other open positions so, please feel free to call me at 866-797-8361 or to send your resume for consideration. Tommy Vedilago System 1 Search (864) 627-0012 (864) 627-0013 Fax From jkiernan <@t> uwo.ca Wed Aug 27 15:36:03 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] solochrome cyanine References: Message-ID: <3F4D1633.8E8C118@uwo.ca> Sigma-Aldrich sell it under the name eriochrome cyanine R or Mordant blue 3. The dye has several synonyms. Make sure the Colour Index number is 43820 and you'll have the right stuff. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ __________________________________________________- > ----- Original Message ----- > From: Patty Lott > To: 'histonet@lists.utsouthwestern.edu' > Sent: Wednesday, August 27, 2003 9:01 AM > Subject: [Histonet] solochrome cyanine > > Does anyone know where to order solochrome cyanine? > > Patty Lott, Laboratory Supervisor > > Orthopaedic Research Laboratory > > Center for Metabolic Bone Disease Laboratory > > University of Alabama at Birmingham > > LHRB B37 0007 > > 1919 7th Ave. South > > Birmingham, AL 35294 > > (205) 934-2007 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Wed Aug 27 15:39:10 2003 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure Message-ID: Jackie, I think I've got an easier solution for you. pack an overnight bag and start driving toward Charleston (you won't need much in the way of clothing here) we'll find a place for you in the lab here and those little BBs will be someone else's headache. by the time they realize you're gone you'll already be far enough away that you won't have to hear the crying and whining. thought you should at least consider ALL of your options. Hey sometimes I find myself fantasizing about running away but then I realize I am already here in Charleston and where else would I go??? Vinnie >>> 08/27/03 08:28AM >>> Fellow histonetters: I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years - so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance. Jackie O'Connor From Amy30histo <@t> cs.com Wed Aug 27 17:44:59 2003 From: Amy30histo <@t> cs.com (Amy30histo@cs.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] test Message-ID: From Jonsorger <@t> aol.com Wed Aug 27 19:51:16 2003 From: Jonsorger <@t> aol.com (Jonsorger@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] most popular IHC antibodies Message-ID: <55837348.26B22F92.025E6E5D@aol.com> I am looking to find out the 'top ten' antibodies that are used for IHC. Does anybody know of a source for such information? I think I know which ones are widely used but would love some real data or a citation. Thanks, Jon From jstaruk <@t> masshistology.com Wed Aug 27 20:07:29 2003 From: jstaruk <@t> masshistology.com (Mass Histology Service) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Rhode Island help wanted (FT & PT) In-Reply-To: <20030827193541.28636.qmail@mail1.superlink.net> Message-ID: We're looking for a full-timer and part-timer to join our rapidly growing histopathology department. This histology lab is as diverse as histology labs come, processing veterinarian, biotechnical, museum, marine, human and research specimens. Everything from artificial hearts to whale hearts are processed here. Never a dull day. GLP, immunohistochemistry and frozen section experience will be very helpful. Supervisory capacity in the near future for the full-timer. Blue-jeans and scrub-top is the recommended dress code but perfection is mandatory. Very flexible hours. Thanks Jim ____________________________ James E. Staruk, HT(ASCP) Mass Histology Service (A division of DVMLab, Inc.) www.masshistology.com From Rohan.Walker <@t> newcastle.edu.au Wed Aug 27 20:23:41 2003 From: Rohan.Walker <@t> newcastle.edu.au (Rohan Walker) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubcribe Message-ID: Rohan Walker Doctoral Student Laboratory of Neuroimmunology University of Newcastle Callghan, N.S.W. Australia. ------------------------------------------------ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/b14bdbfa/attachment.htm From Rohan.Walker <@t> newcastle.edu.au Wed Aug 27 22:22:13 2003 From: Rohan.Walker <@t> newcastle.edu.au (Rohan Walker) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: Rohan Walker Doctoral Student Laboratory of Neuroimmunology University of Newcastle Callghan, N.S.W. Australia. ------------------------------------------------ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/7b6a0540/attachment.htm From Jason.PALMER <@t> svhm.org.au Thu Aug 28 00:21:41 2003 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] RE: formalin blues / mouse CD31 IHC Message-ID: Hi all. I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me). Much obliged! Jason Palmer Bernard O'Brien Institute of Microsurgery Melbourne, Australia Date: Mon, 25 Aug 2003 10:30:55 -0700 From: Christian Franci To: Subject: [Histonet] Formalin blues I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris -------------- next part -------------- A non-text attachment was scrubbed... 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From doscwk <@t> nus.edu.sg Thu Aug 28 00:43:40 2003 From: doscwk <@t> nus.edu.sg (Chan Wai Kam) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Re: processing polymers Message-ID: Hi Linda, I'm from the experimental surgery unit of the dept. of Orthopaedic Surgery and some of our projects right now use scaffolds(poly carbolactone, poly-lactic and poly-glycolic) for the study of tissue repair. Our usual processing and paraffin embedding procedures dissolves away the scaffold. Would you know of any procedures for paraffin sections we could use that would leave the scaffold intact so that it can be viewed in slides. Thanks Julee Chan National University of Singapore -----Original Message----- From: Linda Jenkins [mailto:jlinda@ces.clemson.edu] Sent: Thursday, August 28, 2003 2:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: processing polymers Ian, You wrote: "Does anybody have any helpful hints for processing and paraffin embedding polymers such as poly caprolactone, polyurethanes, polylactide etc. Thanks, Ian Birchall, Melbourne." We do lots of embedding of polymers and have found it depends entirely on the polymer. Some are light, heat, and chemically reactive. Then you have to play "Merlin-the-Magician"! Seriously, are you sectioning beads, scaffolds or gels? I have a PowerPoint handout I can send you if you would be interested? Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From t.hacker <@t> har.mrc.ac.uk Thu Aug 28 02:40:49 2003 From: t.hacker <@t> har.mrc.ac.uk (Terry Hacker) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: <001d01c36d37$b3265ad0$e0f1f682@A383R28DELL360> Terry Hacker Head of Histology and Electron Microscopy Services Medical Research Council Harwell Didcot Oxfordshire OX11 ORD Tel: 01235 841128 Fax: 01235 841200 e-mail t.hacker@har.mrc.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/c4e3dad5/attachment.htm From przemko <@t> med.kuleuven.ac.be Thu Aug 28 02:55:20 2003 From: przemko <@t> med.kuleuven.ac.be (przemko) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubsribe Message-ID: <3F4DB568.7000703@med.kuleuven.ac.be> -- Przemko Tylzanowski Ph.D. LSD & Joint O & N University of Leuven Herestraat 49 3000 Leuven Belgium phone: (32-16)34-61-96 fax : (32-16)34-62-00 From Rohan.Walker <@t> newcastle.edu.au Thu Aug 28 02:54:25 2003 From: Rohan.Walker <@t> newcastle.edu.au (Rohan Walker) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: Rohan Walker Doctoral Student Laboratory of Neuroimmunology University of Newcastle Callghan, N.S.W. Australia. ------------------------------------------------ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/2814cf0d/attachment.htm From angela.mcnabola.b <@t> bayer.com Thu Aug 28 05:41:01 2003 From: angela.mcnabola.b <@t> bayer.com (Angela McNabola) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] RE: formalin blues / mouse CD31 IHC Message-ID: Hi, We, too, in the past have had our issues with CD31 on FFPE tissue (mouse xenographs) to be specific. Thanks to a great technician in Tennessee that I met at a DAKO training course (don't know if she is out there), shared a protocol with me which works wonderfully!!!! We use the Santa Cruz goat (NOT the rabbit) polyclonal PECAM-1 Cat # sc-1506. We do everything on DAKO's immunostainer. Basically here goes, we target retrieval in Dako TRS for approx 20/20, avidin-biotin block (Vector, 10 min each) then we use Vector's goat elite kit. We have found that the anitbody diluted at 1:750 works best for us (The person that helped me recommended 1:400) and we do a 1 hour incubation at RT (or two 30 minute steps on the stainer.) If you need anymor specifics, please feel free to contact me directly. Hope this helps! Angela McNabola MS, HT(ASCP)SLS Bayer Helathcare 400 Morgan Lane West Haven, CT 06516 203-812-5001 "PALMER Jason (SVHM)" To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] RE: formalin blues / mouse CD31 IHC western.edu 08/28/2003 01:21 AM Hi all. I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me). Much obliged! Jason Palmer Bernard O'Brien Institute of Microsurgery Melbourne, Australia Date: Mon, 25 Aug 2003 10:30:55 -0700 From: Christian Franci To: Subject: [Histonet] Formalin blues I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris (See attached file: winmail.dat) (See attached file: InterScan_Disclaimer.txt) -------------- next part -------------- A non-text attachment was scrubbed... Name: winmail.dat Type: application/octet-stream Size: 5274 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/386e9416/winmail.obj -------------- next part -------------- A non-text attachment was scrubbed... Name: InterScan_Disclaimer.txt Type: application/octet-stream Size: 391 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/386e9416/InterScan_Disclaimer.obj From angela.mcnabola.b <@t> bayer.com Thu Aug 28 05:53:17 2003 From: angela.mcnabola.b <@t> bayer.com (Angela McNabola) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] RE: formalin blues / mouse CD31 IHC Message-ID: Sorry....one more important step....we 3% hydrgen peroxide block (made fresh) for 5 miunutes right after antigen retrieval. Angela McNabola To: 08/28/2003 06:41 cc: histonet@lists.utsouthwestern.edu, histonet-admin@lists.utsouthwestern.edu AM Subject: Re: [Histonet] RE: formalin blues / mouse CD31 IHC(Document link: Angela McNabola) Hi, We, too, in the past have had our issues with CD31 on FFPE tissue (mouse xenographs) to be specific. Thanks to a great technician in Tennessee that I met at a DAKO training course (don't know if she is out there), shared a protocol with me which works wonderfully!!!! We use the Santa Cruz goat (NOT the rabbit) polyclonal PECAM-1 Cat # sc-1506. We do everything on DAKO's immunostainer. Basically here goes, we target retrieval in Dako TRS for approx 20/20, avidin-biotin block (Vector, 10 min each) then we use Vector's goat elite kit. We have found that the anitbody diluted at 1:750 works best for us (The person that helped me recommended 1:400) and we do a 1 hour incubation at RT (or two 30 minute steps on the stainer.) If you need anymor specifics, please feel free to contact me directly. Hope this helps! Angela McNabola MS, HT(ASCP)SLS Bayer Helathcare 400 Morgan Lane West Haven, CT 06516 203-812-5001 "PALMER Jason (SVHM)" To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] RE: formalin blues / mouse CD31 IHC western.edu 08/28/2003 01:21 AM Hi all. I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me). Much obliged! Jason Palmer Bernard O'Brien Institute of Microsurgery Melbourne, Australia Date: Mon, 25 Aug 2003 10:30:55 -0700 From: Christian Franci To: Subject: [Histonet] Formalin blues I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris (See attached file: winmail.dat) (See attached file: InterScan_Disclaimer.txt) -------------- next part -------------- A non-text attachment was scrubbed... Name: winmail.dat Type: application/octet-stream Size: 5274 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/0b112c2c/winmail.obj -------------- next part -------------- A non-text attachment was scrubbed... Name: InterScan_Disclaimer.txt Type: application/octet-stream Size: 391 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/0b112c2c/InterScan_Disclaimer.obj From Jackie.O'Connor <@t> abbott.com Thu Aug 28 06:36:30 2003 From: Jackie.O'Connor <@t> abbott.com (Jackie.O'Connor@abbott.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure Message-ID: Sounds great - however I'm writing this from my laptop on a direct flight from Chicago to Bora Bora. I've brought the BB's along with me to soak in South Pacific seawater (saline). Thanks for the offer, however. Charleston will sound a lot more enticing around January in Chicago. Jackie "Vinnie Della Speranza" 08/27/2003 03:39 PM To: , cc: Subject: Re: [Histonet] Processor failure Jackie, I think I've got an easier solution for you. pack an overnight bag and start driving toward Charleston (you won't need much in the way of clothing here) we'll find a place for you in the lab here and those little BBs will be someone else's headache. by the time they realize you're gone you'll already be far enough away that you won't have to hear the crying and whining. thought you should at least consider ALL of your options. Hey sometimes I find myself fantasizing about running away but then I realize I am already here in Charleston and where else would I go??? Vinnie >>> 08/27/03 08:28AM >>> Fellow histonetters: I haven't done this in years. I came in this a.m. to find the tissues on my processor suspended in air over the first absolute alcohol station. Apparently, the machine, a Shandon Citadel 2000, was moved too close to a wall and stuck as it tried to rotate. I have about 100 xenografts to rescue. Right now they all look and feel like bb's. My plan is to rehydrate them and reprocess them, but I have my doubts as to the quality of the morphology and any subsequent IHC. As I said, I haven't screwed up a basket of tissue in years and years - so I would appreciate any new ideas on possibly saving these tumors. Thanks in advance. Jackie O'Connor -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/6dc06809/attachment.htm From jlinda <@t> ces.clemson.edu Thu Aug 28 08:03:40 2003 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Re: processing polymers Message-ID: <5.1.0.14.2.20030828085429.00a98308@mailhost.ces.clemson.edu> Julee, You asked: "I'm from the experimental surgery unit of the dept. of Orthopaedic Surgery and some of our projects right now use scaffolds(poly carbolactone, poly-lactic and poly-glycolic) for the study of tissue repair. Our usual processing and paraffin embedding procedures dissolves away the scaffold. Would you know of any procedures for paraffin sections we could use that would leave the scaffold intact so that it can be viewed in slides." Actually, I do. It is quite common for the scaffold to dissolve in routine paraffin techniques. We have found that doing a frozen section OR processing and embedding in glycol methacrylate(depending on the polymer) works well. I will send you a copy of my PowerPoint presentation. I prefer to do a pilot study on each polymer to determine what works best. I do paraffin, frozen, glycol and methyl methacrylate processing and embedding on each type of polymer...usually one will work just fine. Regards, Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo/histo.htm From juan.gutierrez <@t> christushealth.org Thu Aug 28 08:18:13 2003 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] most popular IHC antibodies Message-ID: Not long ago I got such a survey from Feedbackstat.com. You might want to contact them about it. Good luck. Juan C. Gutierrez, HT(ASCP) -----Original Message----- From: Jonsorger@aol.com [mailto:Jonsorger@aol.com] Sent: Wed 8/27/2003 7:51 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] most popular IHC antibodies I am looking to find out the 'top ten' antibodies that are used for IHC. Does anybody know of a source for such information? I think I know which ones are widely used but would love some real data or a citation. Thanks, Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmt175 <@t> psu.edu Thu Aug 28 08:25:03 2003 From: jmt175 <@t> psu.edu (Jocelyn Torcolini) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: <002301c36d67$ca6399d0$e555ba92@Zavata> -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/8fda4d0e/attachment.htm From gcallis <@t> montana.edu Thu Aug 28 08:59:52 2003 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] RE: formalin blues / mouse CD31 IHC In-Reply-To: Message-ID: <3.0.6.32.20030828075952.00b63ea0@gemini.msu.montana.edu> We use frozen sections avoiding FFPE entirely. Check Histonet Archives on at Histosearch website. There have been messages about many times in the past. Check out this answer from Barb Wright. **************************************************************************** ***** Re: Immunos on Mouse tissue From: Barbara Wright If you are using the DAKO CD31 antibody - it will not react with mouse endothelium. Pharmigen CD31 (MEC13.3) works well on FFPE with Vectastain Elite- ABC followed by NEN TSA-kit. And our Collagen IV is from a polyclonal from Chemicon. Both work well on FFPE mouse tissues. Barb Wright Donella.Stillings@UCHSC.edu wrote: > I am in need of some help? I am trying to do immunos on mouse tissue. I > currently use the Dako LSAB+ kit and I know that some of my antibodies are > monoclonal. I have tried the Dako ARK kit and have not had any luck. I don't > know what I may have done wrong. I also have tried the Dako Envision > polyclonal kit. I have changed my antibodies from mono to poly or goat. I > have tried different retrieval methods. What can I do now? Any help would be > appreciated. The two antibodies I am working with mostly is the Cd31 (goat > polyclonal and Rat anti mouse) and Collagen 4 (monoclonal and polyclonal). > Thanks > Donella Stillings ---------------------------------------------------------------------------- --- > Hi all. > > > I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me). > > Much obliged! > > Jason Palmer > Bernard O'Brien Institute of Microsurgery > Melbourne, Australia > > > Date: Mon, 25 Aug 2003 10:30:55 -0700 > From: Christian Franci > To: > Subject: [Histonet] Formalin blues > > I'm studying neo-vascularization. > It seems that the only antibodies to CD31 (murine and Human) that I can find > commercially (BD Bio and Chemicon) do not work at all for formalin fixed, > paraffin imbedded tissue sections. > > Does anyone out there know of any anti CD31 Ab's that DO work in formalin > fixed tissues? > > Also, does anyone know of any Ab that I could use as a positive control (for > both mouse and human tissues) that works well in formalin-fixed samples? > > Lastly, does anyone have a better fixation method to formalin (compatible > with paraffin imbedding) which may allow me to use the above mentioned > commercial Ab's? > > Thanks for your help! > Cheers, > Chris > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman S. 19th and Lincoln St Bozeman MT 59717-3610 406 994-6367 (lab with voice mail) 406 994-4303 (FAX) From angela.mcnabola.b <@t> bayer.com Thu Aug 28 09:20:02 2003 From: angela.mcnabola.b <@t> bayer.com (Angela McNabola) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] RE: formalin blues / mouse CD31 IHC Message-ID: ----- Forwarded by Angela McNabola/WESTH/PH/US/BAYER on 08/28/2003 10:19 AM ----- Angela McNabola To: 08/28/2003 06:41 cc: histonet@lists.utsouthwestern.edu, histonet-admin@lists.utsouthwestern.edu AM Subject: Re: [Histonet] RE: formalin blues / mouse CD31 IHC(Document link: Angela McNabola) Hi, We, too, in the past have had our issues with CD31 on FFPE tissue (mouse xenographs) to be specific. Thanks to a great technician in Tennessee that I met at a DAKO training course (don't know if she is out there), shared a protocol with me which works wonderfully!!!! We use the Santa Cruz goat (NOT the rabbit) polyclonal PECAM-1 Cat # sc-1506. We do everything on DAKO's immunostainer. Basically here goes, we target retrieval in Dako TRS for approx 20/20, avidin-biotin block (Vector, 10 min each) then we use Vector's goat elite kit. We have found that the anitbody diluted at 1:750 works best for us (The person that helped me recommended 1:400) and we do a 1 hour incubation at RT (or two 30 minute steps on the stainer.) If you need anymor specifics, please feel free to contact me directly. Hope this helps! Angela McNabola MS, HT(ASCP)SLS Bayer Helathcare 400 Morgan Lane West Haven, CT 06516 203-812-5001 "PALMER Jason (SVHM)" To: Sent by: cc: histonet-admin@lists.utsouth Subject: [Histonet] RE: formalin blues / mouse CD31 IHC western.edu 08/28/2003 01:21 AM Hi all. I'm also trying to get an anti mouse CD31 to work on FFPE sections (CBL 1337 from Cymbus / Chemicon). Has anybody had success of any sort with this antibody and type of tissue, and if so, what's your secret? (By the the way, Dako anti human CD31 (M 0823) works just fine on human FFPE tissues for me). Much obliged! Jason Palmer Bernard O'Brien Institute of Microsurgery Melbourne, Australia Date: Mon, 25 Aug 2003 10:30:55 -0700 From: Christian Franci To: Subject: [Histonet] Formalin blues I'm studying neo-vascularization. It seems that the only antibodies to CD31 (murine and Human) that I can find commercially (BD Bio and Chemicon) do not work at all for formalin fixed, paraffin imbedded tissue sections. Does anyone out there know of any anti CD31 Ab's that DO work in formalin fixed tissues? Also, does anyone know of any Ab that I could use as a positive control (for both mouse and human tissues) that works well in formalin-fixed samples? Lastly, does anyone have a better fixation method to formalin (compatible with paraffin imbedding) which may allow me to use the above mentioned commercial Ab's? Thanks for your help! Cheers, Chris (See attached file: winmail.dat) (See attached file: InterScan_Disclaimer.txt) -------------- next part -------------- A non-text attachment was scrubbed... Name: winmail.dat Type: application/octet-stream Size: 5274 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/0e9225fd/winmail.obj -------------- next part -------------- A non-text attachment was scrubbed... Name: InterScan_Disclaimer.txt Type: application/octet-stream Size: 391 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/0e9225fd/InterScan_Disclaimer.obj From algranth <@t> u.arizona.edu Thu Aug 28 10:32:24 2003 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Processor failure In-Reply-To: Message-ID: <4.3.2.7.2.20030828081853.00c74d50@algranth.inbox.email.arizona.edu> Jackie, I hope you are having luck rehydrating the BB's. Just a thought and I don't have the procedure at hand but some time ago there was an article (or maybe workshop) that included a way to hydrate old museum specimens and parts of mummies. Maybe somebody knows about this? There is also something called Ruffer's solution or Zimmerman's that is a combination of 50 parts water, 30 parts absolute alcohol and 20 parts of a 5% Aqueous Sodium Carbonate. Page 33, Freida Carson's book, 2nd edition. Good luck!! Send us a e-postcard from Bora Bora. Andi Grantham At 06:36 AM 8/28/2003 -0500, you wrote: >Sounds great - however I'm writing this from my laptop on a direct flight >from Chicago to Bora Bora. I've brought the BB's along with me to soak in >South Pacific seawater (saline). Thanks for the offer, >however. Charleston will sound a lot more enticing around January in Chicago. > >Jackie > > >"Vinnie Della Speranza" > >08/27/2003 03:39 PM > > To: , > > cc: > Subject: Re: [Histonet] Processor failure > > >Jackie, >I think I've got an easier solution for you. >pack an overnight bag and start driving toward Charleston (you won't need >much in the way of clothing here) we'll find a place for you in the lab >here and those little BBs will be someone else's headache. by the time >they realize you're gone you'll already be far enough away that you won't >have to hear the crying and whining. >thought you should at least consider ALL of your options. > >Hey sometimes I find myself fantasizing about running away but then I >realize I am already here in Charleston and where else would I go??? > >Vinnie > > > > >>> 08/27/03 08:28AM >>> >Fellow histonetters: >I haven't done this in years. I came in this a.m. to find the tissues on >my processor suspended in air over the first absolute alcohol station. >Apparently, the machine, a Shandon Citadel 2000, was moved too close to a >wall and stuck as it tried to rotate. I have about 100 xenografts to >rescue. Right now they all look and feel like bb's. My plan is to >rehydrate them and reprocess them, but I have my doubts as to the quality >of the morphology and any subsequent IHC. As I said, I haven't screwed up >a basket of tissue in years and years - so I would appreciate any new >ideas on possibly saving these tumors. Thanks in advance. > >Jackie O'Connor > > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Robert.Lott <@t> bhsala.com Thu Aug 28 12:01:47 2003 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] HT Readiness Teleconference Question... Message-ID: <35B6C610DD1DD311B1FA0008C791400407F2FFFC@gobexchm3.bhsala.com> Hi Everyone, I'm sorry to bother everyone with this.... but after the I completed the NSH teleconference last week on HT Exam Readiness (on August 20th), I received a question (via e-mail) from a lady in Cape Girardeau, MO. I have tried to answer her question with a response... but keep getting one of those "dreaded" System Admin... Undeliverable messages... the e-mail address does not exist! I have carefully checked that I correctly replied and even copied and pasted the address...but to no avail. If anyone is on the list from there... or even nearby, please get in touch. Thanks! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 (fax) robert.lott@bhsala.com From cquinlan <@t> wlgore.com Thu Aug 28 12:45:28 2003 From: cquinlan <@t> wlgore.com (Christie M Quinlan) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] D-dimer antibody in FFPE tissues Message-ID: Hi all, I was wondering if anyone has had any success using a D-dimer antibody against FFPE tissues. If so, I would love to know your source and method. Thanks, Christie Quinlan W.L. Gore & Assoc. 928-864-3938 From EEARLE <@t> phxmem.com Thu Aug 28 14:41:29 2003 From: EEARLE <@t> phxmem.com (Elizabeth Earle) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Birmingham! Message-ID: <20B916789A4FD411AFEE0008C7916D1C05745E@PMH_EXCHANGE> Anybody working at University of Alabama at Birmingham? My mother will be having surgery there, and I have a lot of questions about where to stay, etc. And of course who are the best doctors? Unbiased opinions, of course! Also, I'd love to visit all the histo labs possible while I'm there. Thanks Elizabeth Earle From histosci <@t> shentel.net Thu Aug 28 15:24:47 2003 From: histosci <@t> shentel.net (HSRL) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] In need of RNAse free sectioning Message-ID: <000401c36da2$72214b60$0200a8c0@HSRLMAIN> Dear Netters, Is there anyone contract labs out there interested in the following study: Frozen sectioning of human liver samples (in an RNAse free environment). According to the researcher, the tissues may be infected with Hepatitis B and C. For more information, please contact Betty Conde at 301.846-7533. Thanks for your help. Tom Galati Laboratory Director HSRL- A GLP Compliant Laboratory 137 South Main Street Woodstock, Virginia 22664 (540)459-8211 Fax: (540)459-8217 tomgalati@hsrl.org www.hsrl.org -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/d1a43300/attachment.htm From mark.lewis <@t> thermo.com Thu Aug 28 15:33:39 2003 From: mark.lewis <@t> thermo.com (mark.lewis@thermo.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Microtomy on wood Message-ID: Does anyone have experiencing in performing microtomy on wood ? If so, would you share the specifics with me ? Thanks ! Best regards, Mark From JLE <@t> rice.willmar.mn.us Thu Aug 28 15:31:19 2003 From: JLE <@t> rice.willmar.mn.us (Jennifer Englin) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Weak progesterone staining, 1A6, Ventana Message-ID: Has anyone noticed any weakening in their progesterone staining with the 1A6 antibody from Ventana? We have been getting good results with this antibody and our standard procedure until recently, when it has become very weak. Fixation has not changed much, although we have tried to regulate this more tightly. Our procedure is the same for both the Nexes and ES: Antigen retrieval- Dako Target: 100 degrees C in a steamer for 20 min and cool down 20 min Our protocol is: Antibody (predilute) 32 min Amplifier A/B Block i-view detection kit We have tried retrieval with several different times- zero, 10 min, 20 min, and 40 min. 40 min works best but is still very weak. Any suggestions would be very welcome. Jennifer Englin, HT(ASCP),PA Rice Memorial Hospital Willmar, MN From algranth <@t> u.arizona.edu Thu Aug 28 17:01:37 2003 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Microtomy on wood In-Reply-To: Message-ID: <4.3.2.7.2.20030828145213.02005a60@algranth.inbox.email.arizona.edu> Mark, Steve Ruzin has a book on plants, Plant Microtechnique and Microscopy, which has a lot of info on wood from fixation to sectioning and staining and including how to soften it up. Also there is another book - Plant Cell Biology edited by Chris Hawes and Beatrice Satiat-Jeunemaitre, where you might find some helpful information. I have never had the pleasure of processing and cutting wood but I'm working on a project with seeds and they are also very hard. I have just completed the processing and microtomy awaits! Good luck! Andi Grantham At 04:33 PM 8/28/2003 -0400, you wrote: >Does anyone have experiencing in performing microtomy on wood ? >If so, would you share the specifics with me ? > > >Thanks ! > > > >Best regards, > >Mark > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From YDHoffman <@t> aol.com Thu Aug 28 17:42:24 2003 From: YDHoffman <@t> aol.com (YDHoffman@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubribe Message-ID: <1cf.1009794a.2c7fdf50@aol.com> at current address: YDHoffman@aol.com subcribe: spencer2748@yahoo.com Thank you in advance, Yvonne Hoffman -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/c5754dd4/attachment.htm From andersen <@t> ohsu.edu Thu Aug 28 18:42:15 2003 From: andersen <@t> ohsu.edu (Donald Andersen) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: From peggy <@t> nsh.org Thu Aug 28 19:43:46 2003 From: peggy <@t> nsh.org (Peggy Micciche) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] NSH S/C Reminder Message-ID: <000301c36dc6$9bc161c0$7300000a@histo2> To all Histonetters who plan to attend the NSH Annual S/C in Louisville, Ky, October 18-23, 2003 RE: HOUSING The deadline for Housing for the Symposium/Convention is September 17th. Rooms cannot be guaranteed at the convention rate after this deadline. A housing form can be found in the S/C Program book, on-line at: www.nsh.org, or call 301-262-6221 and a form can be faxed to you. Thank you, NSH Convention Committee From DiaWingate <@t> aol.com Thu Aug 28 21:28:30 2003 From: DiaWingate <@t> aol.com (DiaWingate@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: Fwd: [Histonet] unsubscribe Message-ID: <1a9.194cc5c6.2c80144e@aol.com> -------------- next part -------------- An embedded message was scrubbed... From: "Jocelyn Torcolini" Subject: [Histonet] unsubscribe Date: Thu, 28 Aug 2003 09:25:03 -0400 Size: 6618 Url: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/fddf2d61/attachment.eml From DiaWingate <@t> aol.com Thu Aug 28 21:28:58 2003 From: DiaWingate <@t> aol.com (DiaWingate@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: <181.1fbd0953.2c80146a@aol.com> Skipped content of type multipart/alternative-------------- next part -------------- A non-text attachment was scrubbed... Name: not available Type: image/jpeg Size: 5263 bytes Desc: not available Url : http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030828/bfc2c856/attachment.jpg From jkiernan <@t> uwo.ca Fri Aug 29 00:01:43 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Microtomy on wood References: <4.3.2.7.2.20030828145213.02005a60@algranth.inbox.email.arizona.edu> Message-ID: <3F4EDE37.8E6DB2C5@uwo.ca> Ruzin's big-page paperback book Plant Microtechnique ... is very good. There is also an older, less expensive hardback from Univ of Iowa Press by Graeme Berlyn, who is now a Professor of Forestry at Yale. Go to http://www.yale.edu/forestry/media/pollution.html Berlyn's textbook provides a step-by-step approach to intelligent staining of sections of parts of plants. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ _____________________- Andrea Grantham wrote: > > Mark, > Steve Ruzin has a book on plants, Plant Microtechnique and Microscopy, > which has a lot of info on wood from fixation to sectioning and staining > and including how to soften it up. Also there is another book - Plant Cell > Biology edited by Chris Hawes and Beatrice Satiat-Jeunemaitre, where you > might find some helpful information. I have never had the pleasure of > processing and cutting wood but I'm working on a project with seeds and > they are also very hard. I have just completed the processing and microtomy > awaits! > Good luck! > Andi Grantham > > At 04:33 PM 8/28/2003 -0400, you wrote: > > >Does anyone have experiencing in performing microtomy on wood ? > >If so, would you share the specifics with me ? > > > > > >Thanks ! > > > > > > > >Best regards, > > > >Mark > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From t.hacker <@t> har.mrc.ac.uk Fri Aug 29 04:34:08 2003 From: t.hacker <@t> har.mrc.ac.uk (Terry Hacker) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] unsubscribe Message-ID: <001c01c36e10$b266a7b0$e0f1f682@A383R28DELL360> Terry Hacker Head of Histology and Electron Microscopy Services Medical Research Council Harwell Didcot Oxfordshire OX11 ORD Tel: 01235 841128 Fax: 01235 841200 e-mail t.hacker@har.mrc.ac.uk -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030829/e45f6b66/attachment.htm From ree3 <@t> leicester.ac.uk Fri Aug 29 06:01:26 2003 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] smear fixation for immunostaining Message-ID: Two ??s, which is the fixative of choice for cell preparations??, the cells(human bladder after purification) will be smeared onto APES coated slides, and then fixed, (ideally if we get enough cell we will make a pellet and process it) and secondly a much easier question, how long is a piece of string? Many thanks Richard Edwards MRC TOXICOLOGY UNIT LEICESTER....U.K......... From stancelb <@t> msn.com Fri Aug 29 14:32:11 2003 From: stancelb <@t> msn.com (Barbara Stancel) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] job and position descriptions Message-ID: Claye, Here another site that may have usable information: http://jobsearch.usajobs.opm.gov/jobsearch.asp?q=Pathology+Technician&re=0&sort=rv&tm=&FedEmp=N&vw=d&brd=3876&ss=0&FedPub=Y Histologically yours, Barbara Barbara H. Stancel _________________________________________________________________ Enter for your chance to IM with Bon Jovi, Seal, Bow Wow, or Mary J Blige using MSN Messenger http://entertainment.msn.com/imastar From Robert.Lott <@t> bhsala.com Fri Aug 29 14:33:05 2003 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Alpha-amylase for PAS w/digestion Message-ID: <35B6C610DD1DD311B1FA0008C791400407E143A5@gobexchm3.bhsala.com> For those of you that use alpha-amylase... "exactly" what kind of reagent do you order for this procedure? Thanks! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Baptist Health System 800 Montclair Road Birmingham, AL 35213 205-592-5388|205-592-5646 (fax) robert.lott@bhsala.com From MTitford <@t> aol.com Fri Aug 29 14:50:51 2003 From: MTitford <@t> aol.com (MTitford@aol.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Alpha Amylase Message-ID: <0C35FE6D.254D1F8A.00762DB1@aol.com> Robert Lotts asks about amylase: We use Sigma Alpha amylase Cat # A-3176.(0.125% at 37 degrees centigrade for 30 minutes). I zap it in the microwave to get it up to temperature Mike Titford USA Pathology Mobile AL 36617 USA From subratab <@t> bdonline.com Fri Aug 29 15:06:42 2003 From: subratab <@t> bdonline.com (Subratab) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] counting positive cells in IHC slides Message-ID: <200308292009.h7TK9hQt012468@korotoa.bdonline.com> Dear All, I am sorry to bother you with a very silly question. I am new in the field of IHC. I have stained rat renal tissue slides for macrophage (ED1) with DAKO EnVision detection system. Now I have to count number of macrophages (ED1 positive cells) per 100 glomeruli. My problem is that the all positive stained cells are not similar to look; some cells are typical cell-like with bright red color, but other cells are a bit different in shape or size or color. So I am in problem in identifying true positive cells. I like to ask you if there is any systematic way to identify true positive cells from false staining area or artefact. ED1 antibody binds with cytoplasmic lysosomal membrane-associated antigen. I treated my slides with 0.3% triton x100 to break the cell membranes for better penetration of the antibody. So I think that the shape of the positive stained cells may be changed and all of them may not look typical cell-like structure. Am I correct? Can you please explain in some detail about the change of shape/size/color of the positive stained cells in IHC slides after staining. Particularly when the antigen is cytoplasmic. Please let me know if there is any website discussing this issue. Thanks in advance Dr Subrata Biswas, MD PhD student, Nephrology Div of Internal Med, FCM, University of Campinas, SP, Brazil. From tpmorken <@t> labvision.com Fri Aug 29 15:10:16 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Alpha Amylase Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CB0E@usca0082k03.rallansci.apogent.com> I used that also, and made it up in aliquots which were frozen for future use. That made the method faster than making up fresh. Tim Morken Lab Vision www.labvision.com -----Original Message----- From: MTitford@aol.com [mailto:MTitford@aol.com] Sent: Friday, August 29, 2003 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Alpha Amylase Robert Lotts asks about amylase: We use Sigma Alpha amylase Cat # A-3176.(0.125% at 37 degrees centigrade for 30 minutes). I zap it in the microwave to get it up to temperature Mike Titford USA Pathology Mobile AL 36617 USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rtarmen <@t> drizzle.com Fri Aug 29 15:26:38 2003 From: rtarmen <@t> drizzle.com (rtarmen@drizzle.com) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Mast vs eosinophils Message-ID: <50762.140.142.125.83.1062188798.squirrel@drizzlemail.drizzle.com> Hi everyone, I need input on determining between mast and eosinophils. I am staining formalin fixed paraffin embedded human scar tissue with acidified tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells that I believe are eosinophils, and I only want mast cells with intact granulation. Any suggestions? _cheers, Rebecca Armendariz Harborview Medical Center Univerisity of Washington, Seattle rtarmen@drizzle.com From bryand <@t> netbistro.com Fri Aug 29 16:05:00 2003 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Mast vs eosinophils References: <50762.140.142.125.83.1062188798.squirrel@drizzlemail.drizzle.com> Message-ID: <002701c36e71$37b25620$ab70c2cf@bryand> Go to http://members.pgonline/~bryand/StainsFile/stain/cell/aldtolblue.htm. This method should do what you want. Bryan Llewellyn ----- Original Message ----- From: To: Sent: Friday, August 29, 2003 1:26 PM Subject: [Histonet] Mast vs eosinophils > Hi everyone, > > I need input on determining between mast and eosinophils. I am staining > formalin fixed paraffin embedded human scar tissue with acidified > tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells > that I believe are eosinophils, and I only want mast cells with intact > granulation. > > Any suggestions? > > _cheers, > > Rebecca Armendariz > Harborview Medical Center > Univerisity of Washington, Seattle > rtarmen@drizzle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bryand <@t> netbistro.com Fri Aug 29 16:12:11 2003 From: bryand <@t> netbistro.com (Bryan Llewellyn) Date: Fri Sep 16 15:21:48 2005 Subject: [Histonet] Mast vs eosinophils References: <50762.140.142.125.83.1062188798.squirrel@drizzlemail.drizzle.com> Message-ID: <003801c36e72$3937ef40$ab70c2cf@bryand> Sorry, I left out part of the URL. The one below should work. http://members.pgonline.com/~bryand/StainsFile/stain/cell/aldtolblue.htm Bryan Llewellyn ----- Original Message ----- From: To: Sent: Friday, August 29, 2003 1:26 PM Subject: [Histonet] Mast vs eosinophils > Hi everyone, > > I need input on determining between mast and eosinophils. I am staining > formalin fixed paraffin embedded human scar tissue with acidified > tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells > that I believe are eosinophils, and I only want mast cells with intact > granulation. > > Any suggestions? > > _cheers, > > Rebecca Armendariz > Harborview Medical Center > Univerisity of Washington, Seattle > rtarmen@drizzle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shar <@t> u.washington.edu Fri Aug 29 16:38:39 2003 From: shar <@t> u.washington.edu (Sharon McLaughlin) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] standard for H&E staining and special stains Message-ID: Is there a standard list out there for how many minutes it takes to do an H&E stain and also the special staining? We are trying to get a standard set up for our lab for billing purposes and for work load credits. Also is there a list of what stains go into which grouping? I know there is a grouping with 1 through 5. We also need to know a standard for cutting blocks. How many minutes it should take for cutting. Thanks for your input. Sharon McLaughlin Neuropathology Harborview Medical Center e From tpmorken <@t> labvision.com Fri Aug 29 16:56:31 2003 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] standard for H&E staining and special stains Message-ID: <8CD0CDC791DCC343ABB1F15298E4F3C417CB11@usca0082k03.rallansci.apogent.com> Sharon, there used to be a CAP comparison survey that had a workload standard that assigned minutes to each procedure. However, it became so complicated in trying to account for everything that it became unworkable. It is not used officially anymore, but some labs may use the old numbers. I think you will be better off to set your own workload times - they vary so much between labs that others times won't necessarily mean anything unless they detail exactly how each procedure is done. Tim Morken -----Original Message----- From: Sharon McLaughlin [mailto:shar@u.washington.edu] Sent: Friday, August 29, 2003 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] standard for H&E staining and special stains Is there a standard list out there for how many minutes it takes to do an H&E stain and also the special staining? We are trying to get a standard set up for our lab for billing purposes and for work load credits. Also is there a list of what stains go into which grouping? I know there is a grouping with 1 through 5. We also need to know a standard for cutting blocks. How many minutes it should take for cutting. Thanks for your input. Sharon McLaughlin Neuropathology Harborview Medical Center e _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Aug 29 23:43:32 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] Re: Mast cells vs eosinophils (LONG) References: <50762.140.142.125.83.1062188798.squirrel@drizzlemail.drizzle.com> Message-ID: <3F502B74.3D6E2946@uwo.ca> Expect a lot of replies from Histonet! Here's mine for what it's worth. Toluidine blue at pH 4 will not stain eosinophil granules - only their nuclei. Mast cell granules will stain strongly, and if you do the technique carefully they will be metachromatic (red) even in the permanently mounted preparation. With less than perfect technique the mast cell granules will be purple, or even dark blue. Nuclei of all cells will also be blue if you stain at pH 4. Cytoplasmic RNA and mucus will also stain strongly, but they may not be present at high concentration in scar tissue. (But what organ? and what sort of scar?) If you stain at a lower pH (such as 1) cell nuclei will not stain and mast cell granules may be the only things visible in the section. You could counterstain everything else pink with a dilute solution of a red anionic dye such as eosin Y. Eosinophils granules will be stained; they'll be the only eosinophilic objects if you use an alkaline solution (pH>8)of the anionic dye. I've done a lot of mast cell staining. For quantitative evaluation of intact vs degranulating cells in rat skin and respiratory tissues my recommendation is toluidine blue pH 4 because (a) the light blue background allows you to see if even a single red granule has been expelled from the cell, and (b) the presence of the blue nucleus shows that the section passes roughly through the centre of the cell. In quantitative work it seems wise to disregard fragments of mast cells (little foci of metachromatic granules) that do not have nuclei. If you are not worried about mast cells that have discharged a few granules, and simply want to count mast cells that are largely intact, or to see generally whether mast cells are present or absent, toluidine blue pH 4 is not the best stain. You won't want to scan large areas of section at high magnification. Instead you should stain the mast cells with alcian blue at pH 1 or 2.5. Use pH 1 if the tissue contains mucus or matrix glycuronoglycans that stain at pH 2.5 and cause confusion. For a background, stain the cell nuclei red. Mayer's brazalum (like haemalum, but made with brazilin instead of haematoxylin) does this very well. This advice is based on my work with mast cells of rats and several other species (including man). Mast cell granules, especially after being ejected into the extracellular fluid, vary in their solubility and fixability. (Have I invented a new word fixability?) Dog and guinea-pig mast cell granules may be easily lost during fixation. In rats they hang around as extracellular dots for many hours. I think humans are rat-like in this way, but don't trust my memory. You should check this up. It's all in Hans Selye's big book, "The Mast Cells," Washington: Butterworths, 1965. Your email indicates that you don't want to know about eosinophils, so I'll refrain from telling you how to stain them selectively. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ _____________________________________ rtarmen@drizzle.com wrote: > > Hi everyone, > > I need input on determining between mast and eosinophils. I am staining > formalin fixed paraffin embedded human scar tissue with acidified > tol-blue-0 (ph 4). I keep getting increased numbers of violet marked cells > that I believe are eosinophils, and I only want mast cells with intact > granulation. > > Any suggestions? > > _cheers, > > Rebecca Armendariz > Harborview Medical Center > Univerisity of Washington, Seattle > rtarmen@drizzle.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Aug 30 00:04:24 2003 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] counting positive cells in IHC slides References: <200308292009.h7TK9hQt012468@korotoa.bdonline.com> Message-ID: <3F503058.589924E@uwo.ca> Your question is not silly. You have 2 problems: 1. Identification of true macrophages. 2. Quantitation of macrophages numbers per renal glomerulus. I cannot help with Problem 1. Your supervisor should look at your slides and show you how to recognize false-positive objects. Problem 2 is more profound. It assumes that you can correctly identify a macrophage. Renal glomeruli are much bigger than macrophages, and the thickness of the sections will influence the apparent abundance of macrophages. In a thick section you will see more macrophages per glomerulus than in a thin section. You need to consult with a statistician BEFORE DOING ANY QUANTITATIVE WORK in this field. If you don't formally consult your institution's experts your work will be rejected by scientific journals, and nobody will ever know what you saw. -- ------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan/ __________________________ Subratab wrote: > > Dear All, > I am sorry to bother you with a very silly question. I am new in the field > of IHC. > I have stained rat renal tissue slides for macrophage (ED1) with DAKO > EnVision detection system. Now I have to count number of macrophages (ED1 > positive cells) per 100 glomeruli. My problem is that the all positive > stained cells are not similar to look; some cells are typical cell-like with > bright red color, but other cells are a bit different in shape or size or > color. So I am in problem in identifying true positive cells. I like to ask > you if there is any systematic way to identify true positive cells from > false staining area or artefact. > ED1 antibody binds with cytoplasmic lysosomal membrane-associated antigen. I > treated my slides with 0.3% triton x100 to break the cell membranes for > better penetration of the antibody. So I think that the shape of the > positive stained cells may be changed and all of them may not look typical > cell-like structure. Am I correct? Can you please explain in some detail > about the change of shape/size/color of the positive stained cells in IHC > slides after staining. Particularly when the antigen is cytoplasmic. Please > let me know if there is any website discussing this issue. Thanks in advance > Dr Subrata Biswas, MD > PhD student, Nephrology Div of Internal Med, > FCM, University of Campinas, SP, Brazil. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akwilliams75 <@t> hotmail.com Sun Aug 31 13:14:45 2003 From: akwilliams75 <@t> hotmail.com (kevin williams) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] Orcein Variation for EVG to detect Elastosis Perforans Serpiginosum Message-ID: An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030831/1a588701/attachment.htm From Linresearch <@t> aol.com Sun Aug 31 16:01:48 2003 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] Nos Abs Message-ID: <4b.3326e80e.2c83bc3c@aol.com> Hello, Does anyone with experience using eNos. iNos and nNOS on FFPE rodent tissue care to share their protocols and AB source/s? Lin -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030831/9492853c/attachment.htm From C.Gorrie <@t> unsw.edu.au Sun Aug 31 19:15:58 2003 From: C.Gorrie <@t> unsw.edu.au (Cathy Gorrie) Date: Fri Sep 16 15:21:49 2005 Subject: [Histonet] counting positive cells in IHC slides In-Reply-To: <200308292009.h7TK9hQt012468@korotoa.bdonline.com> References: <200308292009.h7TK9hQt012468@korotoa.bdonline.com> Message-ID: Dear Subrata, I agree entirely with J.Kiernan's reply. However there are a few steps you may want to take to assist in identifying what your antibody is staining. You don't mention whether if not you used control sections as well. I would suggest a series of at least three. 1. Test tissue with primary omitted. This will show any staining by the cross reactivity by the secondary antibody, any background staining and/or auto fluorescence. Ideally this control should appear NEGATIVE 2. Test tissue with secondary omitted. This will show any autofluorescence in the tissue. Ideally this control should appear NEGATIVE 3. Tissue known to be positive for macrophages, previously identified by other means. This will confirm specificity of your antibody (ED1). Ideally this control should appear POSITIVE for macrophages only These controls will help you establish the range of normal staining patterns for you macrophages and for your tissue. Treat your control sections exactly the same as your test sections except for the omission of antibody. The only thing staining positive should be your macrophages. If there is something else coming up positive you need to be able to identify and/or distinguish between it and macrophages. Cheers, Cathy --------------------------------------------------- Catherine Gorrie School of Medical Sciences University of New South Wales Sydney, N.S.W. 2052 Phone: 61-2-9385 2462 Fax : 61-2-9385 8016 e-mail: c.gorrie@unsw.edu.au 30/8/03, Subratab wrote: >Dear All, >I am sorry to bother you with a very silly question. I am new in the field >of IHC. >I have stained rat renal tissue slides for macrophage (ED1) with DAKO >EnVision detection system. Now I have to count number of macrophages (ED1 >positive cells) per 100 glomeruli. My problem is that the all positive >stained cells are not similar to look; some cells are typical cell-like with >bright red color, but other cells are a bit different in shape or size or >color. So I am in problem in identifying true positive cells. I like to ask >you if there is any systematic way to identify true positive cells from >false staining area or artefact. >ED1 antibody binds with cytoplasmic lysosomal membrane-associated antigen. I >treated my slides with 0.3% triton x100 to break the cell membranes for >better penetration of the antibody. So I think that the shape of the >positive stained cells may be changed and all of them may not look typical >cell-like structure. Am I correct? Can you please explain in some detail >about the change of shape/size/color of the positive stained cells in IHC >slides after staining. Particularly when the antigen is cytoplasmic. Please >let me know if there is any website discussing this issue. Thanks in advance >Dr Subrata Biswas, MD >PhD student, Nephrology Div of Internal Med, >FCM, University of Campinas, SP, Brazil. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Dear --