From kbatts77 <@t> yahoo.com Thu Apr 24 12:20:06 2003 From: kbatts77 <@t> yahoo.com (kenny batts) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] Cryostat section preservation Message-ID: <20030424172006.3557.qmail@web14008.mail.yahoo.com> Hello This is my first time to post, so forgive me in advance.... Would anyone like to give me advice on cryostat preservation? We are cutting 20-30um sections of mouse brains which have either been perfused and postfixed or just perfused. They have been cryoprotected in 20% sucrose for some days, then frozen quickly in -80C 2-methylbutane while in a cryomold with OCT. My question is: What is the best way to handle these sections once they have been cut? We have been told to let them dry for about a day then box them up with some dessicant, put into a ziplock and then into -20 or -80 C freezer. This procedure has been suggested for immunohistochemical staining. For histochemical staining it has been suggested that we simply store the sections in the refrigerator. Is this correct? Do we need to keep slides in the freezer with dessicant? Any help would be greatly appreciated! Kenny Batts Research Tech UT Health Science Center Memphis, TN __________________________________________________ Do you Yahoo!? The New Yahoo! Search - Faster. Easier. Bingo http://search.yahoo.com From Kathy.Smith <@t> dakocytomation.com Tue Apr 29 08:43:01 2003 From: Kathy.Smith <@t> dakocytomation.com (Kathy Smith) Date: Fri Sep 16 15:21:44 2005 Subject: [Histonet] (no subject) Message-ID: <0DCB9F85A742544FBDF4873622E14170082A33@CYTO1> confirm 306719 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20030429/542b1c67/attachment.htm